pycom290g00260

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0006581
Physical Location & Seq
Reverse (-)
164798 .. 165298
501 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom290g00260.1

Sequence Viewer

Length: 501 bp
ATGGAGAGGCAGAGCAGTTTTCAGGCTGCAAGGATGGAAAAACTACCTAGTTTCAGTGGGGCAATTGAGCAGCAGTCAAGTTCTCGAGGGGTGATGGAGAAACAGAAGAGTTTTCGTGGGTTTATGGAGAAGCAGAAGAGTTTTCGGATGGTTATGGAGAAGCAGCTGAGCTTTATTGGTGGCGAACGGAGGAGGAGCAAGGAGTCACCGGGTAAAAGAGGTGACTCTCAACTCCATTTGGCGGCTCGAGCAGGGAATTTAGTTAGAGTCAGAGAAATTCTTCAGAACTGTAACAGCAATGAGTCAATTGGTTTATTGTCAAAGACAAACCAGGAGGGAGAGACCCCCCTCTATGCCTCAGCGGAGAATGGGCATGCTGTGGTTGTTGGGGAAATGTTGAAGCATATGGACCTTCAAACTGCATCAATTGCCGCCAGAAATGGCTACGATCCATTCCACATTGCTGTAAGACAGGGCCATCTTGGTAAGATAGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

167

Amino Acids

18.48

Weight (kDa)

9.93

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 78 - 161 9.5e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 242, 362, 432
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 2 cut(s) 256, 276
AcuI CTGAAG 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 2 cut(s) 400, 416
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 2 cut(s) 166, 171
AluI AGCT 2 cut(s) 166, 171
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 443
Ama87I CYCGRG 2 cut(s) 84, 246
AoxI GGCC 1 cut(s) 475
ApeKI GCWGC 3 cut(s) 26, 70, 163
ApoI RAATTY 2 cut(s) 256, 276
Asp700I GAANNNNTTC 1 cut(s) 279
AspS9I GGNCC 2 cut(s) 409, 475
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 103, 198, 233
AvaI CYCGRG 2 cut(s) 84, 246
AvaII GGWCC 1 cut(s) 409
BbvCI CCTCAGC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 13, 82, 175
BccI CCATC 4 cut(s) 28, 88, 142, 486
BciT130I CCWGG 1 cut(s) 332
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 1 cut(s) 48
BisI GCNGC 5 cut(s) 27, 71, 164, 243, 432
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 5 cut(s) 28, 72, 165, 244, 433
Bme1390I CCNGG 2 cut(s) 210, 332
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 2 cut(s) 84, 246
BmgT120I GGNCC 2 cut(s) 409, 475
BmrFI CCNGG 2 cut(s) 210, 332
BmsI GCATC 1 cut(s) 431
Bpu10I CCTNAGC 1 cut(s) 358
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 335
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse3DI GCAATG 2 cut(s) 304, 459
BseBI CCWGG 1 cut(s) 332
BseGI GGATG 2 cut(s) 39, 153
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 2 cut(s) 304, 459
BseMII CTCAG 2 cut(s) 158, 372
BseRI GAGGAG 2 cut(s) 205, 208
BseXI GCAGC 3 cut(s) 13, 82, 175
BshFI GGCC 1 cut(s) 477
BsiHKCI CYCGRG 2 cut(s) 84, 246
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 335
BsnI GGCC 1 cut(s) 477
Bso31I GGTCTC 1 cut(s) 335
BsoBI CYCGRG 2 cut(s) 84, 246
Bsp143I GATC 1 cut(s) 448
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 3 cut(s) 242, 362, 432
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 2 cut(s) 159, 371
BspPI GGATC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 304, 459
BssMI GATC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 101, 131
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 167, 358
BstF5I GGATG 2 cut(s) 39, 153
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 2 cut(s) 248, 428
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 208, 330
BstV1I GCAGC 3 cut(s) 13, 82, 175
BsuRI GGCC 1 cut(s) 477
BtsCI GGATG 2 cut(s) 39, 153
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 375
Cfr13I GGNCC 2 cut(s) 409, 475
CviAII CATG 1 cut(s) 374
CviJI RGCY 6 cut(s) 26, 166, 171, 245, 444, 477
CviKI_1 RGCY 6 cut(s) 26, 166, 171, 245, 444, 477
DdeI CTNAG 2 cut(s) 167, 358
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
Eam1104I CTCTTC 2 cut(s) 101, 131
EarI CTCTTC 2 cut(s) 101, 131
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 2 cut(s) 84, 246
EcoRII CCWGG 1 cut(s) 330
FaeI CATG 1 cut(s) 377
FaiI YATR 7 cut(s) 125, 155, 354, 375, 405, 407, 499
FatI CATG 1 cut(s) 373
FauNDI CATATG 1 cut(s) 405
Fnu4HI GCNGC 5 cut(s) 27, 71, 164, 243, 432
FokI GGATG 2 cut(s) 46, 160
Fsp4HI GCNGC 5 cut(s) 27, 71, 164, 243, 432
FspBI CTAG 1 cut(s) 48
GluI GCNGC 5 cut(s) 27, 71, 164, 243, 432
HaeIII GGCC 1 cut(s) 477
HapII CCGG 1 cut(s) 209
Hin1II CATG 1 cut(s) 377
HinfI GANTC 4 cut(s) 203, 224, 267, 302
HpaII CCGG 1 cut(s) 209
HphI GGTGA 3 cut(s) 103, 198, 233
Hpy188I TCNGA 3 cut(s) 147, 272, 285
Hpy188III TCNNGA 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 29, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 428
HpyF3I CTNAG 2 cut(s) 167, 358
Hsp92II CATG 1 cut(s) 377
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 1 cut(s) 195
LpnPI CCDG 7 cut(s) 8, 222, 237, 317, 344, 448, 458
Lsp1109I GCAGC 3 cut(s) 13, 82, 175
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 3 cut(s) 204, 221, 290
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 3 cut(s) 118, 148, 272
MfeI CAATTG 3 cut(s) 63, 306, 426
MluCI AATT 5 cut(s) 63, 256, 276, 306, 426
MlyI GAGTC 4 cut(s) 212, 218, 276, 311
MnlI CCTC 7 cut(s) 80, 183, 186, 212, 328, 359, 367
MroXI GAANNNNTTC 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 166, 362
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 210, 332
MunI CAATTG 3 cut(s) 63, 306, 426
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 248, 428
NciI CCSGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 405
NdeII GATC 1 cut(s) 448
NlaIII CATG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 204, 221
NspI RCATGY 1 cut(s) 377
PaeI GCATGC 1 cut(s) 377
PaeR7I CTCGAG 2 cut(s) 84, 246
PdmI GAANNNNTTC 1 cut(s) 279
PkrI GCNGC 5 cut(s) 28, 72, 165, 244, 433
PleI GAGTC 4 cut(s) 211, 218, 275, 310
PpsI GAGTC 4 cut(s) 211, 218, 275, 310
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspPI GGNCC 2 cut(s) 409, 475
PspXI VCTCGAGB 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 166
SatI GCNGC 5 cut(s) 27, 71, 164, 243, 432
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 2 cut(s) 409, 475
SchI GAGTC 4 cut(s) 212, 218, 276, 311
ScrFI CCNGG 2 cut(s) 210, 332
SetI ASST 5 cut(s) 49, 168, 173, 223, 414
SfaNI GCATC 1 cut(s) 431
Sfr274I CTCGAG 2 cut(s) 84, 246
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 2 cut(s) 84, 246
SmlI CTYRAG 2 cut(s) 84, 246
SmoI CTYRAG 2 cut(s) 84, 246
SphI GCATGC 1 cut(s) 377
Sse9I AATT 5 cut(s) 63, 256, 276, 306, 426
SsiI CCGC 3 cut(s) 242, 362, 432
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 208, 330
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 85, 247
TasI AATT 5 cut(s) 63, 256, 276, 306, 426
TauI GCSGC 2 cut(s) 245, 434
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 2 cut(s) 204, 221
TseI GCWGC 3 cut(s) 26, 70, 163
Tsp45I GTSAC 2 cut(s) 204, 221
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 2 cut(s) 256, 276
XceI RCATGY 1 cut(s) 377
XhoI CTCGAG 2 cut(s) 84, 246
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.