MD14G1088100.v1.1

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006581
Physical Location & Seq
Reverse (-)
11028631 .. 11030769
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1088100.v1.1.491

Sequence Viewer

Length: 831 bp
ATGACTGTTGATCGCGCAAACACCACGGCATTGCACATCGCTGCAGCACAGGGACACACTGAAGTAGTAAGTTTTCTTTTGGAGACTGGTTGCAGCTTGATGTCCATAGCAAGAAGCAATGGGAAAACTGCCTTGCATTCTGCAGCAGGGAAGGGGCATTTGGAGGTGGTCCAGGCCCTCTTGAGCAAAGATACCGGATCTGCAATAAGAAAAGATAGTAATGGCCAGACAGCTCTCCATATGGCAGTCAAGGGACAGAATGTCGAATTGGTGGATGAGTTAGCGAAGGCAAATCCTTCGTTGATAAACGAGGTAGACAACAAGGGAAACACTGCCTTGCACGTGGCAACCGGGAAGGGTCGAGCTCAGATTGTTCGAACGCTGTTAAGTTATAAGGGAGTGGCGAAAACCATCATTAACAGATCTGGAGAATCTATTTTTGACACTGCTGAGAGATCTGGGCACTCAAAGATTGCCAACATTCTAGAAGAGCATGGAATCCGTGCAGTTTTTTCAAACTACAATTTGTGCAATATGTGCAATTTTTTTCAAACTGCAATCTGTGATTTTTTTCATACTGGAATCTGTGTAAACTGTAAACTGGAATCTGTGCAGTTTTTTCAAACTGCAATCTGTGTAGCAAACATTTTTAAACAGCAAAAAAAAAACAAAAAAAAGACCTGTGTACCAGACCAAACCTGCTCGTTTCAACCGGGCCGAGTAAGGTCCGGTTCCTATTTTCGGAATTGTAATGCCCGGCCCGTCCCCCCAGGTCTCCTCTGGTCCGGTCCCTTTAAAAATGGGCCCAACCCAGCCCGTGCCCAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

277

Amino Acids

29.83

Weight (kDa)

9.43

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 64 1.7e-14 Ankyrin repeats (3 copies)
Ank_KRIT1 PF24521 7 - 87 5.7e-07 KRIT1 ankyrin-repeats domain
Ank_4 PF13637 11 - 61 1e-09 Ankyrin repeats (many copies)
Ank_4 PF13637 45 - 91 3.4e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 68 - 116 3.9e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 71 - 134 3e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 103 - 168 1.7e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 707
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 15
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 223
AcuI CTGAAG 1 cut(s) 81
AcvI CACGTG 1 cut(s) 343
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 1 cut(s) 741
AgsI TTSAA 4 cut(s) 516, 551, 623, 710
AhdI GACNNNNNGTC 1 cut(s) 260
AjnI CCWGG 2 cut(s) 171, 769
AjuI GAANNNNNNNTTGG 4 cut(s) 143, 175, 251, 283
AluBI AGCT 3 cut(s) 96, 233, 365
AluI AGCT 3 cut(s) 96, 233, 365
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 2 cut(s) 77, 779
AlwI GGATC 1 cut(s) 205
AoxI GGCC 5 cut(s) 174, 223, 715, 758, 803
ApaI GGGCCC 1 cut(s) 807
ApeKI GCWGC 4 cut(s) 41, 44, 93, 143
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 9 cut(s) 169, 175, 715, 726, 759, 783, 788, 803, 804
AsuC2I CCSGG 3 cut(s) 352, 714, 757
AsuII TTCGAA 1 cut(s) 376
AvaII GGWCC 4 cut(s) 169, 726, 783, 788
BaeGI GKGCMC 3 cut(s) 465, 807, 823
BaeI ACNNNNGTAYC 2 cut(s) 669, 702
BalI TGGCCA 1 cut(s) 225
BanII GRGCYC 2 cut(s) 367, 807
BbrPI CACGTG 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 4 cut(s) 28, 56, 105, 155
BccI CCATC 1 cut(s) 419
BceAI ACGGC 1 cut(s) 42
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 2 cut(s) 173, 771
BcnI CCSGG 3 cut(s) 352, 714, 757
BcoDI GTCTC 2 cut(s) 77, 779
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 2 cut(s) 42, 141
BfuAI ACCTGC 1 cut(s) 707
BglII AGATCT 2 cut(s) 422, 455
BisI GCNGC 4 cut(s) 42, 45, 94, 144
BlsI GCNGC 4 cut(s) 43, 46, 95, 145
Bme1390I CCNGG 5 cut(s) 173, 352, 714, 757, 771
Bme18I GGWCC 4 cut(s) 169, 726, 783, 788
BmeRI GACNNNNNGTC 1 cut(s) 260
BmgT120I GGNCC 9 cut(s) 169, 175, 715, 726, 759, 783, 788, 803, 804
BmiI GGNNCC 3 cut(s) 733, 790, 805
BmrFI CCNGG 5 cut(s) 173, 352, 714, 757, 771
BpmI CTGGAG 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 202
BpuMI CCSGG 3 cut(s) 352, 714, 757
BsaAI YACGTR 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 24, 769
BsaWI WCCGGW 3 cut(s) 194, 728, 785
Bsc4I CCNNNNNNNGG 1 cut(s) 741
Bse1I ACTGG 3 cut(s) 91, 583, 606
Bse3DI GCAATG 2 cut(s) 29, 124
BseBI CCWGG 2 cut(s) 173, 771
BseDI CCNNGG 2 cut(s) 24, 769
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 741
BseMI GCAATG 2 cut(s) 29, 124
BseMII CTCAG 2 cut(s) 380, 441
BseNI ACTGG 3 cut(s) 91, 583, 606
BseRI GAGGAG 1 cut(s) 767
BseSI GKGCMC 3 cut(s) 465, 807, 823
BseXI GCAGC 4 cut(s) 28, 56, 105, 155
BseYI CCCAGC 2 cut(s) 811, 822
BsgI GTGCAG 2 cut(s) 525, 632
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 5 cut(s) 176, 225, 717, 760, 805
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 6 cut(s) 195, 351, 713, 729, 757, 786
BslFI GGGAC 4 cut(s) 66, 267, 749, 774
BslI CCNNNNNNNGG 1 cut(s) 741
BsmAI GTCTC 2 cut(s) 77, 779
BsmFI GGGAC 4 cut(s) 66, 267, 749, 774
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 5 cut(s) 176, 225, 717, 760, 805
Bso31I GGTCTC 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 376
Bsp120I GGGCCC 1 cut(s) 803
Bsp1286I GDGCHC 4 cut(s) 367, 465, 807, 823
Bsp143I GATC 4 cut(s) 10, 197, 422, 455
BspANI GGCC 5 cut(s) 176, 225, 717, 760, 805
BspCNI CTCAG 2 cut(s) 379, 442
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 3 cut(s) 733, 790, 805
BspMAI CTGCAG 2 cut(s) 46, 145
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 779
BsrDI GCAATG 2 cut(s) 29, 124
BsrI ACTGG 3 cut(s) 91, 583, 606
BssECI CCNNGG 2 cut(s) 24, 769
BssMI GATC 4 cut(s) 10, 197, 422, 455
Bst2UI CCWGG 2 cut(s) 173, 771
Bst4CI ACNGT 2 cut(s) 7, 596
Bst6I CTCTTC 1 cut(s) 483
BstAPI GCANNNNNTGC 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 343
BstBI TTCGAA 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 366, 450
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 4 cut(s) 13, 200, 425, 458
BstMAI GTCTC 2 cut(s) 77, 779
BstMBI GATC 4 cut(s) 10, 197, 422, 455
BstMWI GCNNNNNNNGC 1 cut(s) 537
BstNI CCWGG 2 cut(s) 173, 771
BstSCI CCNGG 5 cut(s) 171, 350, 712, 755, 769
BstSFI CTRYAG 2 cut(s) 42, 141
BstSLI GKGCMC 3 cut(s) 465, 807, 823
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 4 cut(s) 28, 56, 105, 155
BstX2I RGATCY 3 cut(s) 197, 422, 455
BstYI RGATCY 3 cut(s) 197, 422, 455
BsuRI GGCC 5 cut(s) 176, 225, 717, 760, 805
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 22
BtsCI GGATG 1 cut(s) 280
BtsI GCAGTG 2 cut(s) 330, 444
BtsIMutI CAGTG 3 cut(s) 57, 330, 444
BveI ACCTGC 1 cut(s) 707
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 9 cut(s) 169, 175, 715, 726, 759, 783, 788, 803, 804
Csp6I GTAC 1 cut(s) 686
CviAII CATG 1 cut(s) 494
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 366, 450
DpnI GATC 4 cut(s) 12, 199, 424, 457
DpnII GATC 4 cut(s) 10, 197, 422, 455
DraI TTTAAA 2 cut(s) 652, 796
DriI GACNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 483
Eam1105I GACNNNNNGTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 365
Eco24I GRGCYC 2 cut(s) 367, 807
Eco31I GGTCTC 1 cut(s) 779
Eco47I GGWCC 4 cut(s) 169, 726, 783, 788
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 81
Eco72I CACGTG 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 171, 769
EcoT38I GRGCYC 2 cut(s) 367, 807
FaeI CATG 1 cut(s) 497
FaiI YATR 7 cut(s) 107, 240, 242, 393, 495, 536, 576
FaqI GGGAC 4 cut(s) 66, 267, 749, 774
FatI CATG 1 cut(s) 493
FauNDI CATATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 42, 45, 94, 144
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 2 cut(s) 367, 807
Fsp4HI GCNGC 4 cut(s) 42, 45, 94, 144
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 16
GluI GCNGC 4 cut(s) 42, 45, 94, 144
GsaI CCCAGC 2 cut(s) 815, 826
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 5 cut(s) 176, 225, 717, 760, 805
HapII CCGG 6 cut(s) 195, 351, 713, 729, 757, 786
HhaI GCGC 1 cut(s) 17
Hin1II CATG 1 cut(s) 497
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 4 cut(s) 431, 498, 582, 605
HpaII CCGG 6 cut(s) 195, 351, 713, 729, 757, 786
Hpy166II GTNNAC 4 cut(s) 316, 592, 599, 686
Hpy188I TCNGA 2 cut(s) 369, 744
Hpy188III TCNNGA 3 cut(s) 181, 426, 485
Hpy8I GTNNAC 4 cut(s) 316, 592, 599, 686
HpyAV CCTTC 4 cut(s) 145, 280, 306, 349
HpyCH4III ACNGT 2 cut(s) 7, 596
HpyCH4IV ACGT 1 cut(s) 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 537
HpyF3I CTNAG 2 cut(s) 366, 450
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 1 cut(s) 497
HspAI GCGC 1 cut(s) 15
Kzo9I GATC 4 cut(s) 10, 197, 422, 455
LguI GCTCTTC 1 cut(s) 483
Lsp1109I GCAGC 4 cut(s) 28, 56, 105, 155
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 342
MalI GATC 4 cut(s) 12, 199, 424, 457
MboI GATC 4 cut(s) 10, 197, 422, 455
MboII GAAGA 1 cut(s) 500
MflI RGATCY 3 cut(s) 197, 422, 455
MhlI GDGCHC 4 cut(s) 367, 465, 807, 823
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 4 cut(s) 266, 523, 541, 745
MluNI TGGCCA 1 cut(s) 225
MnlI CCTC 4 cut(s) 157, 188, 304, 788
Mox20I TGGCCA 1 cut(s) 225
MscI TGGCCA 1 cut(s) 225
MseI TTAA 4 cut(s) 386, 417, 651, 795
Msp20I TGGCCA 1 cut(s) 225
MspI CCGG 6 cut(s) 195, 351, 713, 729, 757, 786
MspR9I CCNGG 5 cut(s) 173, 352, 714, 757, 771
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 2 cut(s) 173, 771
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 537
NciI CCSGG 3 cut(s) 352, 714, 757
NdeI CATATG 1 cut(s) 240
NdeII GATC 4 cut(s) 10, 197, 422, 455
NlaIII CATG 1 cut(s) 497
NlaIV GGNNCC 3 cut(s) 733, 790, 805
NmeAIII GCCGAG 1 cut(s) 743
NspV TTCGAA 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 483
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 4 cut(s) 431, 498, 582, 605
PkrI GCNGC 4 cut(s) 43, 46, 95, 145
PmaCI CACGTG 1 cut(s) 343
PmlI CACGTG 1 cut(s) 343
Ppu21I YACGTR 1 cut(s) 343
PsiI TTATAA 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 171, 769
PspCI CACGTG 1 cut(s) 343
PspFI CCCAGC 2 cut(s) 811, 822
PspGI CCWGG 2 cut(s) 171, 769
PspN4I GGNNCC 3 cut(s) 733, 790, 805
PspOMI GGGCCC 1 cut(s) 803
PspPI GGNCC 9 cut(s) 169, 175, 715, 726, 759, 783, 788, 803, 804
PstI CTGCAG 2 cut(s) 46, 145
PsuI RGATCY 3 cut(s) 197, 422, 455
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
SacI GAGCTC 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 4 cut(s) 386, 417, 651, 795
SatI GCNGC 4 cut(s) 42, 45, 94, 144
Sau3AI GATC 4 cut(s) 10, 197, 422, 455
Sau96I GGNCC 9 cut(s) 169, 175, 715, 726, 759, 783, 788, 803, 804
ScrFI CCNGG 5 cut(s) 173, 352, 714, 757, 771
SduI GDGCHC 4 cut(s) 367, 465, 807, 823
SfcI CTRYAG 2 cut(s) 42, 141
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 4 cut(s) 169, 726, 783, 788
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 4 cut(s) 266, 523, 541, 745
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 5 cut(s) 171, 350, 712, 755, 769
TaaI ACNGT 2 cut(s) 7, 596
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 264, 361, 376
TasI AATT 4 cut(s) 266, 523, 541, 745
TfiI GAWTC 4 cut(s) 431, 498, 582, 605
Tru1I TTAA 4 cut(s) 386, 417, 651, 795
Tru9I TTAA 4 cut(s) 386, 417, 651, 795
TscAI CASTG 3 cut(s) 64, 337, 451
TseI GCWGC 4 cut(s) 41, 44, 93, 143
TspDTI ATGAA 1 cut(s) 563
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 3 cut(s) 64, 337, 451
VpaK11BI GGWCC 4 cut(s) 169, 726, 783, 788
XbaI TCTAGA 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.