pycom290g00240

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0006581
Physical Location & Seq
Reverse (-)
162779 .. 163679
901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom290g00240.1

Sequence Viewer

Length: 846 bp
ATGTTTAAAGAGGGTAGTAACATCAATGCAAGCAACAAGGCTGGAGAGAGCCCACTTGACATTGCAGAAAAATATGGGAGTCCAGAACTTGTGGCCGTGTTAAAGGAAGCAGGGGCCACCAATTCTAAAGACCAAGGAAAACCTGCAAATCCAGCCAAGCAGCTGAAGCAGACTGTGAGTGACATAAAGCACGATGTCCAGTCCCAACTTCAACAGACCCGCCAAACTGGTGCCAGAGTCCAGAATATTGCAAAAAAGTTGAAAAAGCTCCACATTAGCGGCCTCAACAATGCTATAAACTCTGCCACTGTTGTTGCCGTTCTGATTGCCACTGTTGCTTTTGCAGCCATCTTCACTATTCCTGGCGAGTATGTTGAGGACGAAACACCAGGGCTTACGCTTGGACAAGCGCACATAGCAAATAATGCCGCTTTCATCATCTTTTTTGTGTTTGACAGCCTGGCACTCTTCATCTCCCTGGCTGTTGTTGTAGTCCAGACATCTGTGGTTGTGATTGAGCAGAAGGCAAAAAAGCAGCTTGTATTTGTGATCAACAAGCTCATGTGGCTAGCTTGCCTCTTCATTTCGATTGCTTTTATTTCTCTGACGTATGTGGTGGTGGGCACGCACTCGAGGTGGCTTGCTGTGTACGCAACAGTTATAGGCAGCACAATCATGCTTACTACCATTGGCTCCATGTGCTATTGTATCATTTTACATAGGATGGAAGAGTGCAAAATGAGGAACATTCGGAGAGCTGAGAGTAAGTCTCGTTCATTCTCTATGTCTATGGCATCAGAACACGAAGTTTTGAACAGCGAGTATAAGCGGATGTATGCGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

282

Amino Acids

30.82

Weight (kDa)

9.28

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 94 - 206 7.5e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 230
AciI CCGC 4 cut(s) 220, 279, 429, 829
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 1 cut(s) 650
AgsI TTSAA 3 cut(s) 212, 262, 814
AjnI CCWGG 4 cut(s) 361, 388, 459, 477
AluBI AGCT 6 cut(s) 163, 268, 538, 559, 572, 758
AluI AGCT 6 cut(s) 163, 268, 538, 559, 572, 758
Alw26I GTCTC 1 cut(s) 774
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 3 cut(s) 93, 114, 280
ApeKI GCWGC 4 cut(s) 160, 344, 535, 666
AspLEI GCGC 2 cut(s) 412, 841
AspS9I GGNCC 1 cut(s) 114
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 631
BaeGI GKGCMC 1 cut(s) 626
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 4 cut(s) 172, 356, 547, 678
BccI CCATC 2 cut(s) 356, 718
BceAI ACGGC 2 cut(s) 80, 302
BciT130I CCWGG 4 cut(s) 363, 390, 461, 479
BclI TGATCA 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 774
BfaI CTAG 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 151
BisI GCNGC 6 cut(s) 161, 280, 345, 429, 536, 667
BlsI GCNGC 6 cut(s) 162, 281, 346, 430, 537, 668
Bme1390I CCNGG 4 cut(s) 363, 390, 461, 479
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 3 cut(s) 115, 232, 694
BmrFI CCNGG 4 cut(s) 363, 390, 461, 479
BmsI GCATC 1 cut(s) 803
BmtI GCTAGC 1 cut(s) 572
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BpmI CTGGAG 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 133, 389, 477
Bse1I ACTGG 2 cut(s) 199, 232
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 4 cut(s) 363, 390, 461, 479
BseDI CCNNGG 3 cut(s) 133, 389, 477
BseGI GGATG 2 cut(s) 729, 837
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 2 cut(s) 199, 232
BseSI GKGCMC 1 cut(s) 626
BseXI GCAGC 4 cut(s) 172, 356, 547, 678
BshFI GGCC 3 cut(s) 95, 116, 282
BshNI GGYRCC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 774
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 3 cut(s) 95, 116, 282
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 2 cut(s) 53, 626
Bsp143I GATC 1 cut(s) 549
BspACI CCGC 4 cut(s) 220, 279, 429, 829
BspANI GGCC 3 cut(s) 95, 116, 282
BspCNI CTCAG 1 cut(s) 751
BspLI GGNNCC 3 cut(s) 115, 232, 694
BspMI ACCTGC 1 cut(s) 151
BspOI GCTAGC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 2 cut(s) 199, 232
BssECI CCNNGG 3 cut(s) 133, 389, 477
BssMI GATC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 4 cut(s) 363, 390, 461, 479
Bst4CI ACNGT 4 cut(s) 175, 310, 334, 658
Bst6I CTCTTC 3 cut(s) 473, 584, 723
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 5 cut(s) 31, 570, 574, 626, 642
BstDEI CTNAG 1 cut(s) 759
BstF5I GGATG 2 cut(s) 729, 837
BstHHI GCGC 2 cut(s) 412, 841
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 1 cut(s) 774
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 9 cut(s) 152, 166, 335, 344, 416, 425, 565, 650, 699
BstNI CCWGG 4 cut(s) 363, 390, 461, 479
BstSCI CCNGG 4 cut(s) 361, 388, 459, 477
BstSLI GKGCMC 1 cut(s) 626
BstV1I GCAGC 4 cut(s) 172, 356, 547, 678
BsuRI GGCC 3 cut(s) 95, 116, 282
BtsCI GGATG 2 cut(s) 729, 837
BtsIMutI CAGTG 2 cut(s) 306, 330
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 5 cut(s) 31, 570, 574, 626, 642
CfoI GCGC 2 cut(s) 412, 841
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 649
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 3 cut(s) 562, 676, 697
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 1 cut(s) 759
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
DraI TTTAAA 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 3 cut(s) 473, 584, 723
EarI CTCTTC 3 cut(s) 473, 584, 723
Eco130I CCWWGG 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 631
EcoRII CCWGG 4 cut(s) 361, 388, 459, 477
EcoT14I CCWWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 3 cut(s) 565, 679, 700
FaqI GGGAC 1 cut(s) 187
FatI CATG 3 cut(s) 561, 675, 696
FauI CCCGC 1 cut(s) 227
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 6 cut(s) 161, 280, 345, 429, 536, 667
FokI GGATG 1 cut(s) 736
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 6 cut(s) 161, 280, 345, 429, 536, 667
FspBI CTAG 1 cut(s) 569
GlaI GCGC 2 cut(s) 411, 840
GluI GCNGC 6 cut(s) 161, 280, 345, 429, 536, 667
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 3 cut(s) 95, 116, 282
HhaI GCGC 2 cut(s) 412, 841
Hin1II CATG 3 cut(s) 565, 679, 700
Hin6I GCGC 2 cut(s) 410, 839
HinP1I GCGC 2 cut(s) 410, 839
HinfI GANTC 2 cut(s) 79, 237
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 324, 606, 753, 799
Hpy188III TCNNGA 3 cut(s) 83, 241, 496
Hpy8I GTNNAC 1 cut(s) 649
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 4 cut(s) 175, 310, 334, 658
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 6 cut(s) 29, 65, 146, 251, 344, 735
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 166, 335, 344, 416, 425, 565, 650, 699
HpyF3I CTNAG 1 cut(s) 759
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 3 cut(s) 565, 679, 700
HspAI GCGC 2 cut(s) 410, 839
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 1 cut(s) 549
LmnI GCTCC 2 cut(s) 273, 698
Lsp1109I GCAGC 4 cut(s) 172, 356, 547, 678
LweI GCATC 1 cut(s) 803
MaeI CTAG 1 cut(s) 569
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 17, 179
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 4 cut(s) 343, 460, 571, 740
MhlI GDGCHC 2 cut(s) 53, 626
MluCI AATT 1 cut(s) 121
MlyI GAGTC 2 cut(s) 88, 246
MnlI CCTC 6 cut(s) 4, 293, 370, 587, 627, 735
MseI TTAA 3 cut(s) 6, 101, 844
MslI CAYNNNNRTG 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 163
MspR9I CCNGG 4 cut(s) 363, 390, 461, 479
MvaI CCWGG 4 cut(s) 363, 390, 461, 479
MwoI GCNNNNNNNGC 9 cut(s) 152, 166, 335, 344, 416, 425, 565, 650, 699
NdeII GATC 1 cut(s) 549
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 3 cut(s) 565, 679, 700
NlaIV GGNNCC 3 cut(s) 115, 232, 694
NmuCI GTSAC 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 631
PkrI GCNGC 6 cut(s) 162, 281, 346, 430, 537, 668
PleI GAGTC 2 cut(s) 87, 245
PpsI GAGTC 2 cut(s) 87, 245
Psp6I CCWGG 4 cut(s) 361, 388, 459, 477
PspGI CCWGG 4 cut(s) 361, 388, 459, 477
PspN4I GGNNCC 3 cut(s) 115, 232, 694
PspPI GGNCC 1 cut(s) 114
PspXI VCTCGAGB 1 cut(s) 631
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
PvuII CAGCTG 1 cut(s) 163
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
RseI CAYNNNNRTG 1 cut(s) 674
SaqAI TTAA 3 cut(s) 6, 101, 844
SatI GCNGC 6 cut(s) 161, 280, 345, 429, 536, 667
Sau3AI GATC 1 cut(s) 549
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 2 cut(s) 88, 246
ScrFI CCNGG 4 cut(s) 363, 390, 461, 479
SduI GDGCHC 2 cut(s) 53, 626
SetI ASST 9 cut(s) 145, 165, 270, 540, 561, 574, 611, 638, 760
SfaNI GCATC 1 cut(s) 803
Sfr274I CTCGAG 1 cut(s) 631
SlaI CTCGAG 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 1 cut(s) 121
SsiI CCGC 4 cut(s) 220, 279, 429, 829
SspI AATATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 569
StyD4I CCNGG 4 cut(s) 361, 388, 459, 477
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 4 cut(s) 175, 310, 334, 658
TaiI ACGT 1 cut(s) 611
TaqI TCGA 2 cut(s) 587, 632
TasI AATT 1 cut(s) 121
TauI GCSGC 2 cut(s) 282, 431
Tru1I TTAA 3 cut(s) 6, 101, 844
Tru9I TTAA 3 cut(s) 6, 101, 844
TscAI CASTG 2 cut(s) 313, 337
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 4 cut(s) 160, 344, 535, 666
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 4 cut(s) 424, 460, 571, 765
TspRI CASTG 2 cut(s) 313, 337
XhoI CTCGAG 1 cut(s) 631
XspI CTAG 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.