AT2G33585

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009396
Physical Location & Seq
Reverse (-)
14223170 .. 14224167
998 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33585.2

Sequence Viewer

Length: 582 bp
ATGGTAAAACAATGTTCTCCGGCCCACAATATATTTGAGCGGGTCTCCAAATGTTACGTGGAGAAACAACAGCCGCATGATGTGTCAATCCCAAACATCAGATTAACCGTCTCGGCGACCGGTTTAGCCTTTGGCAGTGTTCACCAAGCAAATGCGAATCAAAATCAAATCGCCTGCTTTTACTTTTCTTGCAATTTCAGTTCCAAATCGAATCATCATCAAAGTCAAGCAATTGTCAGAGAAGAGATCCAGAAGAACCTTAGACCAGTTCGTATAGTTGGGCATCAATCAATGGCTAGCAGCTGGAGAAGATCAATTGGAAACGTGAGATCTTTCATCGGTAATTCCATGGGAGGTCTTAGAGGTGGTCAGAGTGCCGCTTCTTGGGTCGTCGCCGGAACTATTGCTTACTTTCTCTGGATTAAGCCAGAACAAGATCTTAAAAAGGAACAAGAGGCTAGGGCAGCTCTTGCAATGGCTGATACTAATCAATACGTTGAGAAGAGGAAACCAATCGCTGACCCTCAGGTTACTGGTTTGATATATGGAAACAAGAACGGAACTGATAAGTCAGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.42

Weight (kDa)

9.48

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 40
AciI CCGC 3 cut(s) 40, 74, 378
AclWI GGATC 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 384
AgeI ACCGGT 1 cut(s) 119
AluBI AGCT 2 cut(s) 303, 467
AluI AGCT 2 cut(s) 303, 467
Alw26I GTCTC 2 cut(s) 49, 115
AlwI GGATC 1 cut(s) 241
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 2 cut(s) 300, 464
AsiGI ACCGGT 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 134
AsuNHI GCTAGC 1 cut(s) 296
AxyI CCTNAGG 1 cut(s) 525
BbvI GCAGC 2 cut(s) 312, 476
BcoDI GTCTC 2 cut(s) 49, 115
BfaI CTAG 2 cut(s) 297, 459
BglII AGATCT 2 cut(s) 329, 436
BisI GCNGC 4 cut(s) 74, 301, 378, 465
BlsI GCNGC 4 cut(s) 75, 302, 379, 466
BmgT120I GGNCC 1 cut(s) 22
BmsI GCATC 1 cut(s) 292
BmtI GCTAGC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 348
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 2 cut(s) 266, 538
Bse21I CCTNAGG 1 cut(s) 525
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 486
BseDI CCNNGG 1 cut(s) 348
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 2 cut(s) 266, 538
BseXI GCAGC 2 cut(s) 312, 476
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 23
BshTI ACCGGT 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 120
BsiSI CCGG 3 cut(s) 20, 120, 396
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 2 cut(s) 49, 115
BsmBI CGTCTC 1 cut(s) 115
BsnI GGCC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 49
Bsp143I GATC 4 cut(s) 246, 311, 329, 436
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 3 cut(s) 40, 74, 378
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 538
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 1 cut(s) 241
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 2 cut(s) 266, 538
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 4 cut(s) 246, 311, 329, 436
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 2 cut(s) 237, 497
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 175, 298
BstDEI CTNAG 3 cut(s) 260, 359, 525
BstDSI CCRYGG 1 cut(s) 348
BstKTI GATC 4 cut(s) 249, 314, 332, 439
BstMAI GTCTC 2 cut(s) 49, 115
BstMBI GATC 4 cut(s) 246, 311, 329, 436
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 464, 470
BstV1I GCAGC 2 cut(s) 312, 476
BstX2I RGATCY 3 cut(s) 246, 329, 436
BstYI RGATCY 3 cut(s) 246, 329, 436
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 348
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 175, 298
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 22
CspAI ACCGGT 1 cut(s) 119
CviAII CATG 2 cut(s) 77, 349
CviJI RGCY 9 cut(s) 23, 73, 128, 296, 303, 427, 458, 467, 479
CviKI_1 RGCY 9 cut(s) 23, 73, 128, 296, 303, 427, 458, 467, 479
DdeI CTNAG 3 cut(s) 260, 359, 525
DpnI GATC 4 cut(s) 248, 313, 331, 438
DpnII GATC 4 cut(s) 246, 311, 329, 436
Eam1104I CTCTTC 2 cut(s) 237, 497
EarI CTCTTC 2 cut(s) 237, 497
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 49
Eco81I CCTNAGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 2 cut(s) 80, 352
FaiI YATR 6 cut(s) 32, 78, 275, 350, 544, 546
FatI CATG 2 cut(s) 76, 348
FauI CCCGC 1 cut(s) 33
Fnu4HI GCNGC 4 cut(s) 74, 301, 378, 465
Fsp4HI GCNGC 4 cut(s) 74, 301, 378, 465
FspBI CTAG 2 cut(s) 297, 459
GluI GCNGC 4 cut(s) 74, 301, 378, 465
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 23
HapII CCGG 3 cut(s) 20, 120, 396
Hin1II CATG 2 cut(s) 80, 352
HinfI GANTC 2 cut(s) 157, 211
HpaII CCGG 3 cut(s) 20, 120, 396
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 4 cut(s) 101, 239, 372, 574
Hpy188III TCNNGA 2 cut(s) 250, 418
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 395
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 3 cut(s) 57, 324, 495
HpyCH4V TGCA 2 cut(s) 192, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 470
HpyF3I CTNAG 3 cut(s) 260, 359, 525
HpySE526I ACGT 3 cut(s) 57, 324, 495
Hsp92II CATG 2 cut(s) 80, 352
Kzo9I GATC 4 cut(s) 246, 311, 329, 436
Lsp1109I GCAGC 2 cut(s) 312, 476
LweI GCATC 1 cut(s) 292
MaeI CTAG 2 cut(s) 297, 459
MaeII ACGT 3 cut(s) 57, 324, 495
MaeIII GTNAC 2 cut(s) 53, 529
MalI GATC 4 cut(s) 248, 313, 331, 438
MbiI CCGCTC 1 cut(s) 40
MboI GATC 4 cut(s) 246, 311, 329, 436
MboII GAAGA 4 cut(s) 254, 265, 321, 514
MfeI CAATTG 2 cut(s) 231, 315
MflI RGATCY 3 cut(s) 246, 329, 436
MluCI AATT 4 cut(s) 193, 231, 315, 343
MnlI CCTC 5 cut(s) 347, 356, 448, 498, 534
MseI TTAA 3 cut(s) 104, 423, 441
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 3 cut(s) 20, 120, 396
MunI CAATTG 2 cut(s) 231, 315
MwoI GCNNNNNNNGC 2 cut(s) 464, 470
NcoI CCATGG 1 cut(s) 348
NdeII GATC 4 cut(s) 246, 311, 329, 436
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 2 cut(s) 80, 352
NmeAIII GCCGAG 1 cut(s) 92
PfeI GAWTC 2 cut(s) 157, 211
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 4 cut(s) 75, 302, 379, 466
Ppu21I YACGTR 1 cut(s) 58
PspPI GGNCC 1 cut(s) 22
PsuI RGATCY 3 cut(s) 246, 329, 436
PvuII CAGCTG 1 cut(s) 303
SaqAI TTAA 3 cut(s) 104, 423, 441
SatI GCNGC 4 cut(s) 74, 301, 378, 465
Sau3AI GATC 4 cut(s) 246, 311, 329, 436
Sau96I GGNCC 1 cut(s) 22
SetI ASST 9 cut(s) 60, 261, 305, 327, 358, 367, 469, 498, 531
SfaNI GCATC 1 cut(s) 292
Sse9I AATT 4 cut(s) 193, 231, 315, 343
SsiI CCGC 3 cut(s) 40, 74, 378
SspMI CTAG 2 cut(s) 297, 459
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 3 cut(s) 60, 327, 498
TaqI TCGA 1 cut(s) 209
TasI AATT 4 cut(s) 193, 231, 315, 343
TauI GCSGC 2 cut(s) 76, 380
TfiI GAWTC 2 cut(s) 157, 211
Tru1I TTAA 3 cut(s) 104, 423, 441
Tru9I TTAA 3 cut(s) 104, 423, 441
TscAI CASTG 1 cut(s) 142
TseI GCWGC 2 cut(s) 300, 464
TspDTI ATGAA 1 cut(s) 325
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XspI CTAG 2 cut(s) 297, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.