AT2G35660

Squalene epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012148
Physical Location & Seq
Forward (+)
14988437 .. 14990426
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35660.1

Sequence Viewer

Length: 1320 bp
ATGTCTGTTCTGCTTCCGATGTATCCTCTCCAATCCTTGGCCAGAAGCAAGAGATTGTTTCCGATTCGAACCAGATCAAAACCGGTTTGTTTAGCACTGACCAATGCTCAAACAAACGGTGGTGACCAAGAGGAGAAAGTAGTGATTGTTGGCGCCGGGATTGGCGGACTTGCCACTGCCGTTTCACTTCACAGGCTTGGAATCCGGTCGGTGGTGTTGGAGCAGGCAGAGTCGCTTCGTACTGGTGGAACATCACTGACGCTATTCAAGAATGGGTGGCGCGTTCTTGACGCCATTTCCGTCGGGCCTCAACTCCGAACGCAGTTTCTTGAAATCGAAGGGATGGTAGTGAAGAAGGAAGATGGAAGAGAGCTTCGTTCTTTCAAATTCAAAGACGATGATCAAAGCCAAGAAGTTCGGGCCGTGGAGAGGCGAGTACTCTTGGAAACACTTGCAAGCCAGCTTCCTCCACAAACCATTCGGTTTTCCTCAAAACTGGAAAGTATACAAAGCAATGCCAATGGTGACACTCTCCTTCAACTTGGAGATGGAACCCGGTTGCTTGCACAGATTGTTATTGGTTGTGACGGTATCCGGTCTAAGGTTGCAACTTGGATGGGGTTCAGTGAGCCGAAATATGTTGGCCATTGCGCTTACCGTGGTCTTGGGTTCTATCCAAATGGACAGCCATTTCAGAAAAAGGTGAACTACATATACGGTAAAGGGATTAGAGCTGGATATGTACCTGTATCTACAACAAAAGTCTACTGGTTCATCTGTTTCAACAGCCCATCTCTAGGGCCCAAAATTACGGATCCAGCGATCCTAAAAAAACAAGCCAAAGAACTGGTTAGTACATGGCCAGAAGATCTGCAAAACCTCATCGATTTAACACCTGATGAAACTATCAGCAGAACTCCTCTAGTAGACCGGTGGCTATGGCCTGGCATTGCTCCTCCAGCATCAAAAGGACGAGTGGTTCTTGTTGGAGATGCTTGGCACCCAATGACTCCAAATCTTGGACAAGGTGCTTGTTGTGCCTTGGAAGATTCAGTAGTTCTGGCAAATAAACTTGCCAATGCAATAAATGGAGGAACCGAATCAATTGAAGTAGCAATGGAATCATACGGGAGTGAGAGATGGTCTCGAGCATTCCCGTTGACAGTACGTGCAAACCTAGTTGGCGCACTTCTGCAATGGGAGAATCCTCTAGTGTGTTCCATTAGAAACAATATTGTCATACCAAAGTTACTGAGGCTTGGACCAATGCTTGAACACACAAACTTTGAGTGTGAACCTCTCTTTGTTTCTAAAAGCTGA

Protein Analysis

439

Amino Acids

48.31

Weight (kDa)

9.1

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 46 - 384 1.7e-26 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35660 AT2G35660 AT2G35660
fragaria_vesca FvH4_5g20080
malus_domestica MD06G1049200.v1.1
prunus_persica Prupe.5G059700_v2.0.a1 Prupe.5G059700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204941
rosa_laevigata RLG00000003420
rosa_multiflora Rmu_co8273435.1_g000001 Rmu_sc0002180.1_g000002
rosa_roxburghii Rroxscaffold_3G00252650
rosa_rugosa Rorug07G0086700 Rorug07G0086800
rosa_samantha Rh7AG217400 Rh7BG214300 Rh7CG230700 Rh7DG225000
rosa_wichuraiana Rw7G018860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 152, 999
AccB7I CCANNNNNTGG 2 cut(s) 37, 1019
AccI GTMKAC 3 cut(s) 505, 765, 927
AccII CGCG 1 cut(s) 282
AciI CCGC 1 cut(s) 165
AclWI GGATC 3 cut(s) 809, 817, 822
AcoI YGGCCR 3 cut(s) 39, 643, 860
AcsI RAATTY 1 cut(s) 386
AcyI GRCGYC 2 cut(s) 153, 291
AfaI GTAC 5 cut(s) 241, 438, 744, 856, 1167
AfiI CCNNNNNNNGG 6 cut(s) 37, 211, 429, 601, 797, 1019
AgeI ACCGGT 2 cut(s) 82, 930
AgsI TTSAA 8 cut(s) 268, 332, 385, 391, 539, 784, 1109, 1274
AjnI CCWGG 1 cut(s) 943
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 4 cut(s) 373, 463, 734, 1317
AluI AGCT 4 cut(s) 373, 463, 734, 1317
Alw26I GTCTC 1 cut(s) 1149
AlwI GGATC 3 cut(s) 809, 817, 822
Ama87I CYCGRG 1 cut(s) 1146
AoxI GGCC 7 cut(s) 39, 305, 420, 643, 800, 860, 941
ApaI GGGCCC 1 cut(s) 804
ApoI RAATTY 1 cut(s) 386
AsiGI ACCGGT 2 cut(s) 82, 930
AspLEI GCGC 4 cut(s) 155, 282, 653, 1187
AspS9I GGNCC 5 cut(s) 305, 420, 800, 801, 1262
AsuC2I CCSGG 2 cut(s) 157, 556
AsuHPI GGTGA 3 cut(s) 134, 536, 715
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 1146
AvaII GGWCC 1 cut(s) 1262
BaeGI GKGCMC 1 cut(s) 804
BalI TGGCCA 3 cut(s) 41, 645, 862
BamHI GGATCC 1 cut(s) 814
BanI GGYRCC 2 cut(s) 152, 999
BanII GRGCYC 1 cut(s) 804
BccI CCATC 6 cut(s) 337, 356, 542, 610, 799, 1134
BceAI ACGGC 2 cut(s) 164, 407
BciT130I CCWGG 1 cut(s) 945
BciVI GTATCC 2 cut(s) 33, 602
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 2 cut(s) 157, 556
BcoDI GTCTC 1 cut(s) 1149
BfaI CTAG 4 cut(s) 797, 923, 1178, 1211
BfoI RGCGCY 1 cut(s) 156
BfuI GTATCC 2 cut(s) 33, 602
BglII AGATCT 1 cut(s) 868
BmcAI AGTACT 1 cut(s) 438
Bme1390I CCNGG 3 cut(s) 157, 556, 945
Bme18I GGWCC 1 cut(s) 1262
BmeT110I CYCGRG 1 cut(s) 1146
BmgT120I GGNCC 5 cut(s) 305, 420, 800, 801, 1262
BmiI GGNNCC 6 cut(s) 154, 553, 802, 816, 1001, 1096
BmrFI CCNGG 3 cut(s) 157, 556, 945
BmsI GCATC 2 cut(s) 971, 982
BplI GAGNNNNNCTC 2 cut(s) 1129, 1161
BpmI CTGGAG 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 67
BpuMI CCSGG 2 cut(s) 157, 556
Bsa29I ATCGAT 1 cut(s) 885
BsaAI YACGTR 1 cut(s) 1169
BsaHI GRCGYC 2 cut(s) 153, 291
BsaI GGTCTC 1 cut(s) 1149
BsaJI CCNNGG 4 cut(s) 36, 423, 658, 1041
BsaWI WCCGGW 4 cut(s) 82, 204, 594, 930
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 211, 429, 601, 797, 1019
Bse118I RCCGGY 2 cut(s) 82, 930
Bse1I ACTGG 4 cut(s) 247, 501, 773, 852
Bse3DI GCAATG 5 cut(s) 520, 646, 948, 1122, 1202
BseBI CCWGG 1 cut(s) 945
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 4 cut(s) 36, 423, 658, 1041
BseGI GGATG 2 cut(s) 348, 621
BseLI CCNNNNNNNGG 6 cut(s) 37, 211, 429, 601, 797, 1019
BseMI GCAATG 5 cut(s) 520, 646, 948, 1122, 1202
BseMII CTCAG 1 cut(s) 1244
BseNI ACTGG 4 cut(s) 247, 501, 773, 852
BseRI GAGGAG 3 cut(s) 146, 909, 945
BseSI GKGCMC 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 7 cut(s) 41, 307, 422, 645, 802, 862, 943
BshNI GGYRCC 2 cut(s) 152, 999
BshTI ACCGGT 2 cut(s) 82, 930
BshVI ATCGAT 1 cut(s) 885
BsiEI CGRYCG 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 1146
BsiSI CCGG 6 cut(s) 83, 156, 205, 556, 595, 931
BslI CCNNNNNNNGG 6 cut(s) 37, 211, 429, 601, 797, 1019
BsmAI GTCTC 1 cut(s) 1149
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 7 cut(s) 41, 307, 422, 645, 802, 862, 943
Bso31I GGTCTC 1 cut(s) 1149
BsoBI CYCGRG 1 cut(s) 1146
Bsp119I TTCGAA 1 cut(s) 67
Bsp120I GGGCCC 1 cut(s) 800
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 5 cut(s) 74, 400, 814, 822, 868
BspACI CCGC 1 cut(s) 165
BspANI GGCC 7 cut(s) 41, 307, 422, 645, 802, 862, 943
BspCNI CTCAG 1 cut(s) 1245
BspDI ATCGAT 1 cut(s) 885
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 6 cut(s) 154, 553, 802, 816, 1001, 1096
BspPI GGATC 3 cut(s) 809, 817, 822
BspT104I TTCGAA 1 cut(s) 67
BspT107I GGYRCC 2 cut(s) 152, 999
BspTNI GGTCTC 1 cut(s) 1149
BsrDI GCAATG 5 cut(s) 520, 646, 948, 1122, 1202
BsrFI RCCGGY 2 cut(s) 82, 930
BsrI ACTGG 4 cut(s) 247, 501, 773, 852
BssAI RCCGGY 2 cut(s) 82, 930
BssECI CCNNGG 4 cut(s) 36, 423, 658, 1041
BssMI GATC 5 cut(s) 74, 400, 814, 822, 868
BssNAI GTATAC 1 cut(s) 506
BssNI GRCGYC 2 cut(s) 153, 291
BssT1I CCWWGG 2 cut(s) 36, 1041
Bst1107I GTATAC 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 5 cut(s) 119, 590, 659, 719, 1165
Bst6I CTCTTC 1 cut(s) 361
BstACI GRCGYC 2 cut(s) 153, 291
BstBAI YACGTR 1 cut(s) 1169
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 4 cut(s) 225, 457, 461, 564
BstDEI CTNAG 2 cut(s) 600, 1253
BstDSI CCRYGG 2 cut(s) 423, 658
BstEII GGTNACC 1 cut(s) 122
BstF5I GGATG 2 cut(s) 348, 621
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 4 cut(s) 155, 282, 653, 1187
BstKTI GATC 5 cut(s) 77, 403, 817, 825, 871
BstMAI GTCTC 1 cut(s) 1149
BstMBI GATC 5 cut(s) 74, 400, 814, 822, 868
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 2 cut(s) 959, 1037
BstNI CCWGG 1 cut(s) 945
BstPI GGTNACC 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 155, 554, 943
BstSLI GKGCMC 1 cut(s) 804
BstUI CGCG 1 cut(s) 282
BstX2I RGATCY 2 cut(s) 814, 868
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 2 cut(s) 814, 868
BstZ17I GTATAC 1 cut(s) 506
Bsu15I ATCGAT 1 cut(s) 885
BsuI GTATCC 2 cut(s) 33, 602
BsuRI GGCC 7 cut(s) 41, 307, 422, 645, 802, 862, 943
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 2 cut(s) 423, 658
BtsCI GGATG 2 cut(s) 348, 621
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 4 cut(s) 95, 174, 254, 631
Cac8I GCNNGC 4 cut(s) 225, 457, 461, 564
CfoI GCGC 4 cut(s) 155, 282, 653, 1187
Cfr10I RCCGGY 2 cut(s) 82, 930
Cfr13I GGNCC 5 cut(s) 305, 420, 800, 801, 1262
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 2 cut(s) 268, 299
Csp6I GTAC 5 cut(s) 240, 437, 743, 855, 1166
CspAI ACCGGT 2 cut(s) 82, 930
CviAII CATG 1 cut(s) 858
CviQI GTAC 5 cut(s) 240, 437, 743, 855, 1166
DdeI CTNAG 2 cut(s) 600, 1253
DinI GGCGCC 1 cut(s) 154
DpnI GATC 5 cut(s) 76, 402, 816, 824, 870
DpnII GATC 5 cut(s) 74, 400, 814, 822, 868
EaeI YGGCCR 3 cut(s) 39, 643, 860
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 36, 1041
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 1149
Eco47I GGWCC 1 cut(s) 1262
Eco88I CYCGRG 1 cut(s) 1146
Eco91I GGTNACC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 800
EcoO65I GGTNACC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 943
EcoT14I CCWWGG 2 cut(s) 36, 1041
EcoT38I GRGCYC 1 cut(s) 804
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 36, 1041
FaeI CATG 1 cut(s) 861
FaiI YATR 9 cut(s) 506, 639, 713, 715, 741, 859, 940, 1126, 1241
FatI CATG 1 cut(s) 857
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 3 cut(s) 505, 765, 927
FokI GGATG 2 cut(s) 355, 628
FriOI GRGCYC 1 cut(s) 804
FspBI CTAG 4 cut(s) 797, 923, 1178, 1211
GlaI GCGC 4 cut(s) 154, 281, 652, 1186
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 7 cut(s) 41, 307, 422, 645, 802, 862, 943
HapII CCGG 6 cut(s) 83, 156, 205, 556, 595, 931
HgaI GACGC 2 cut(s) 268, 299
HhaI GCGC 4 cut(s) 155, 282, 653, 1187
Hin1I GRCGYC 2 cut(s) 153, 291
Hin1II CATG 1 cut(s) 861
Hin6I GCGC 4 cut(s) 153, 280, 651, 1185
HinP1I GCGC 4 cut(s) 153, 280, 651, 1185
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HinfI GANTC 8 cut(s) 64, 201, 230, 1009, 1049, 1100, 1121, 1204
HpaII CCGG 6 cut(s) 83, 156, 205, 556, 595, 931
HphI GGTGA 3 cut(s) 134, 536, 715
Hpy166II GTNNAC 6 cut(s) 506, 706, 766, 928, 1161, 1295
Hpy188I TCNGA 4 cut(s) 18, 63, 317, 696
Hpy188III TCNNGA 4 cut(s) 268, 287, 329, 1146
Hpy8I GTNNAC 6 cut(s) 506, 706, 766, 928, 1161, 1295
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 3 cut(s) 332, 349, 545
HpyCH4III ACNGT 5 cut(s) 119, 590, 659, 719, 1165
HpyCH4IV ACGT 1 cut(s) 1168
HpyCH4V TGCA 7 cut(s) 455, 566, 608, 874, 1082, 1172, 1195
HpyF10VI GCNNNNNNNGC 2 cut(s) 959, 1037
HpyF3I CTNAG 2 cut(s) 600, 1253
HpySE526I ACGT 1 cut(s) 1168
Hsp92I GRCGYC 2 cut(s) 153, 291
Hsp92II CATG 1 cut(s) 861
HspAI GCGC 4 cut(s) 153, 280, 651, 1185
KasI GGCGCC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 74, 400, 814, 822, 868
LmnI GCTCC 2 cut(s) 220, 958
LweI GCATC 2 cut(s) 971, 982
MaeI CTAG 4 cut(s) 797, 923, 1178, 1211
MaeII ACGT 1 cut(s) 1168
MaeIII GTNAC 4 cut(s) 122, 524, 584, 1248
MalI GATC 5 cut(s) 76, 402, 816, 824, 870
MboI GATC 5 cut(s) 74, 400, 814, 822, 868
MboII GAAGA 5 cut(s) 364, 371, 378, 878, 1058
MfeI CAATTG 1 cut(s) 1104
MflI RGATCY 2 cut(s) 814, 868
MhlI GDGCHC 1 cut(s) 804
MlsI TGGCCA 3 cut(s) 41, 645, 862
MluCI AATT 3 cut(s) 386, 807, 1104
MluNI TGGCCA 3 cut(s) 41, 645, 862
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 2 cut(s) 239, 1003
MmeI TCCRAC 2 cut(s) 198, 967
Mox20I TGGCCA 3 cut(s) 41, 645, 862
MscI TGGCCA 3 cut(s) 41, 645, 862
MseI TTAA 1 cut(s) 890
Msp20I TGGCCA 3 cut(s) 41, 645, 862
MspI CCGG 6 cut(s) 83, 156, 205, 556, 595, 931
MspR9I CCNGG 3 cut(s) 157, 556, 945
MunI CAATTG 1 cut(s) 1104
Mva1269I GAATGC 1 cut(s) 1151
MvaI CCWGG 1 cut(s) 945
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 959, 1037
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 2 cut(s) 157, 556
NdeII GATC 5 cut(s) 74, 400, 814, 822, 868
NlaIII CATG 1 cut(s) 861
NlaIV GGNNCC 6 cut(s) 154, 553, 802, 816, 1001, 1096
NmuCI GTSAC 3 cut(s) 122, 524, 584
NspV TTCGAA 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 818
PctI GAATGC 1 cut(s) 1151
PfeI GAWTC 6 cut(s) 64, 201, 1049, 1100, 1121, 1204
PflMI CCANNNNNTGG 2 cut(s) 37, 1019
PinAI ACCGGT 2 cut(s) 82, 930
PleI GAGTC 2 cut(s) 238, 1003
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 2 cut(s) 238, 1003
Ppu21I YACGTR 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 943
PspEI GGTNACC 1 cut(s) 122
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 6 cut(s) 154, 553, 802, 816, 1001, 1096
PspOMI GGGCCC 1 cut(s) 800
PspPI GGNCC 5 cut(s) 305, 420, 800, 801, 1262
PsrI GAACNNNNNNTAC 2 cut(s) 698, 730
PsuI RGATCY 2 cut(s) 814, 868
RsaI GTAC 5 cut(s) 241, 438, 744, 856, 1167
RsaNI GTAC 5 cut(s) 240, 437, 743, 855, 1166
SaqAI TTAA 1 cut(s) 890
Sau3AI GATC 5 cut(s) 74, 400, 814, 822, 868
Sau96I GGNCC 5 cut(s) 305, 420, 800, 801, 1262
ScaI AGTACT 1 cut(s) 438
SchI GAGTC 2 cut(s) 239, 1003
ScrFI CCNGG 3 cut(s) 157, 556, 945
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 2 cut(s) 971, 982
SfoI GGCGCC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 1146
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 1 cut(s) 1262
SlaI CTCGAG 1 cut(s) 1146
SmlI CTYRAG 1 cut(s) 1146
SmoI CTYRAG 1 cut(s) 1146
Sse9I AATT 3 cut(s) 386, 807, 1104
SsiI CCGC 1 cut(s) 165
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 1 cut(s) 1234
SspMI CTAG 4 cut(s) 797, 923, 1178, 1211
StyD4I CCNGG 3 cut(s) 155, 554, 943
StyI CCWWGG 2 cut(s) 36, 1041
TaaI ACNGT 5 cut(s) 119, 590, 659, 719, 1165
TaiI ACGT 1 cut(s) 1171
TaqI TCGA 4 cut(s) 67, 336, 885, 1147
TasI AATT 3 cut(s) 386, 807, 1104
TatI WGTACW 2 cut(s) 436, 854
TfiI GAWTC 6 cut(s) 64, 201, 1049, 1100, 1121, 1204
Tru1I TTAA 1 cut(s) 890
Tru9I TTAA 1 cut(s) 890
TscAI CASTG 4 cut(s) 102, 181, 261, 631
TseFI GTSAC 3 cut(s) 122, 524, 584
Tsp45I GTSAC 3 cut(s) 122, 524, 584
TspDTI ATGAA 2 cut(s) 763, 915
TspGWI ACGGA 2 cut(s) 289, 827
TspRI CASTG 4 cut(s) 102, 181, 261, 631
Van91I CCANNNNNTGG 2 cut(s) 37, 1019
VpaK11BI GGWCC 1 cut(s) 1262
XapI RAATTY 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 1146
XmiI GTMKAC 3 cut(s) 505, 765, 927
XspI CTAG 4 cut(s) 797, 923, 1178, 1211
ZrmI AGTACT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.