Rorug07G0086800

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000044
Physical Location & Seq
Forward (+)
6808041 .. 6812058
4018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0086800.1

Sequence Viewer

Length: 1890 bp
ATGGCTAGGGACTATGGTGGTGGATCACCAAAGCACCACCAGGTTGAATCCAAGAAGAAGAGGCTGACTATGATCTTTGGGGTTAGTGCACTTTGCATAGTATGTTACTGCTTAGGAGCTTGGCAAACCACTTCTGCTCCCGTCAGTCGGTCTGAACTCTACAACAGGGTTGGTTGCGATGAAGTTTCTAGCCAAATGAAGACCTCCAATGCCTCATCGGAGTCTGTTGCACCTCACTTGGATTTCGAAAGCCATCACCAAGTGGATATCAACAAATCCGAGGCAGTCCAGAAGTTCCCACCGTGTGCCATGACGTACAGTGAGTACACTCCTTGCCAGGATCCGAACAGGGGGAGAAAATTCGACAGGAAAATGTTGAAGTACAGAGAGCGGCATTGCCCCACTAAAGAAGAACAACTTCTTTGCTTGATACCGGCTCCACCAAAGTACAAGACTCCTTTTATGTGGCCTCAGAGCCGGGATTTCGCCTGGTATGACAACATTCCGCATAGGGAGCTTAGCATTGAGAAAGCTGTTCAGAATTGGATTCAAGTTGAGGGTGACCGGTTTAGATTCCCTGGTGGAGGTACCATGTTTCCAAAAGGAGCTGATGCTTATATAGATGACATTAATGCGCTCATTCCTCTTACTAAGGGTCAAATCAGAACTGCAATTGACACAGGCTGTGGTGTTGCAAGTTGGGGTGCTTACTTGTTGAAGAGGAATATCTTGACAATGTCTTTTGCTCCAAGAGATACGCATGCAGCACAGGTCCAGTTCGCATTGGAGAGAGGAGTTCCTGCTATGATTGGTGTCATGGGTTCAAAAAGGCTTCCCTACCCGGCCAGGGCTTTCGATATGGCTCACTGTTCCCGCTGCTTGATACCGTGGCAGAATAATGATGGTCTGTATCTAATTGAAGTGGACAGAGTTCTAAGGCCGGGCGCTTACTGGATTCTTTCCGGGCCCCCTATTAACTGGAAAAAACACTGGAGAGGATGGGAGAGAACCCAAGAAGACTTGAAACAAGAGCAAGATGCTATTGAGGCTGTTGCGAAGAGCTTGTGCTGGAAGAAGGTGATTCAGAAGAATGATCTTGCTATCTGGAAAAAGCCTCTCAATCACATTGAATGCATCAGAAGCAGGAATGTTGTTAAGACACCGCATCTATGCAAATCAGACAATCCAGATATGGCTTGGGACAGAAATATGGAAACTTGCATTACACCTCTACCAGAGGCAAACAATCCGAATGACGTTGCTGGTGGGGCATTGGAGAAGTGGCCTGAGCGTGCATTTGCCATCCCACCTAGAATCAGCAGTGGTTCAATACCAGGAATCACCCCGGAGAAACTTAAAGAGGATAATGATCTGTGGAAGCAGAGGATGGAACGTTACAAGCGGATTATACCAATTTCCAAAGGAAGGTATAGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCTGCAGCGTCGAAATATCCAGTCTGGGTTATGAACACAGTTCCTGCCAATACAAACCAGGACACTCTTGGTGTGATTTATGAAAGGGGTTTCATTGGTGCCTACCAGGATTGGTGTGAAGCATTTTCGACATACCCCAGAACTTATGATCTCATCCATGCTGGCGGTGTCTTCAGTATATATCAGGACAGGTGTGACATCACATACATATTGCTGGAAATGGATAGAATTCTGAGGCCAGAAGGAACCGTTATATTTAGGGATACTGTGGAGATACTTACGAAGATAAAGGCCATTACAGATGGGATGAGGTGGAAGAGTCAAATTATGGATCATGAGAGTGGACCTTTTAATCCAGAGAAAATTCTAGTTGCTGTCAAAACCTACTGGACTGGTGAAGCTAATAAGCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

629

Amino Acids

71.73

Weight (kDa)

8.83

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 108 - 618 4.5e-226 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07760 AT5G28823
fragaria_vesca FvH4_1g13572 FvH4_2g03281 FvH4_2g04691 FvH4_2g08332 FvH4_2g08333 FvH4_2g15313 FvH4_2g26101 FvH4_2g29541 FvH4_3g06911 FvH4_3g34351 FvH4_3g43733 FvH4_4g02432 FvH4_4g05601 FvH4_4g08213 FvH4_4g09961 FvH4_4g10131 FvH4_4g20001 FvH4_4g20002 FvH4_5g01051 FvH4_5g01401 FvH4_5g27943 FvH4_6g09220 FvH4_6g17383 FvH4_6g35491 FvH4_6g46421 FvH4_7g04151 FvH4_7g13962 FvH4_7g18611 FvH4_7g19491 FvH4_7g19511 FvH4_7g26562
malus_domestica MD06G1221600.v1.1
pyrus_communis pycom10g21150
rosa_chinensis RchiOBHm_Chr1g0337571 RchiOBHm_Chr2g0168771 RchiOBHm_Chr3g0453631 RchiOBHm_Chr6g0278581
rosa_laevigata RLG00000001899 RLG00000001925 RLG00000002026 RLG00000003410 RLG00000003767 RLG00000004304 RLG00000004473 RLG00000004677 RLG00000006856 RLG00000007994 RLG00000009888 RLG00000011627 RLG00000012174 RLG00000012217 RLG00000013118 RLG00000013736 RLG00000014194 RLG00000014251 RLG00000015011 RLG00000015057 RLG00000015235 RLG00000017416 RLG00000018052 RLG00000018537 RLG00000019968 RLG00000020096 RLG00000020263 RLG00000022606 RLG00000023119 RLG00000028111 RLG00000029686 RLG00000030126 RLG00000031124 RLG00000031236 RLG00000032766 RLG00000033171 RLG00000033548 RLG00000033987 RLG00000034062 RLG00000035115 RLG00000035863
rosa_multiflora Rmu_co8036712.1_g000001 Rmu_co8080248.1_g000001 Rmu_co8208334.1_g000001 Rmu_co8253451.1_g000001 Rmu_co8393241.1_g000001 Rmu_co8522225.1_g000005 Rmu_sc0000060.1_g000030 Rmu_sc0000060.1_g000031 Rmu_sc0000060.1_g000032 Rmu_sc0000090.1_g000008 Rmu_sc0000149.1_g000051 Rmu_sc0000149.1_g000058 Rmu_sc0000309.1_g000050 Rmu_sc0000370.1_g000022 Rmu_sc0000370.1_g000023 Rmu_sc0000370.1_g000024 Rmu_sc0000418.1_g000018 Rmu_sc0000427.1_g000014 Rmu_sc0000427.1_g000015 Rmu_sc0000434.1_g000008 Rmu_sc0000487.1_g000038 Rmu_sc0000487.1_g000039 Rmu_sc0000569.1_g000029 Rmu_sc0000580.1_g000097 Rmu_sc0000595.1_g000068 Rmu_sc0000607.1_g000027 Rmu_sc0000738.1_g000004 Rmu_sc0000758.1_g000001 Rmu_sc0000758.1_g000002 Rmu_sc0000870.1_g000019 Rmu_sc0000924.1_g000040 Rmu_sc0000924.1_g000041 Rmu_sc0000999.1_g000037 Rmu_sc0001001.1_g000001 Rmu_sc0001010.1_g000080 Rmu_sc0001075.1_g000041 Rmu_sc0001097.1_g000016 Rmu_sc0001173.1_g000020 Rmu_sc0001258.1_g000010 Rmu_sc0001425.1_g000031 Rmu_sc0001493.1_g000020 Rmu_sc0001493.1_g000021 Rmu_sc0001644.1_g000024 Rmu_sc0001648.1_g000039 Rmu_sc0001662.1_g000002 Rmu_sc0001849.1_g000046 Rmu_sc0001851.1_g000038 Rmu_sc0001861.1_g000006 Rmu_sc0001899.1_g000008 Rmu_sc0002231.1_g000023 Rmu_sc0002393.1_g000012 Rmu_sc0002515.1_g000010 Rmu_sc0002551.1_g000013 Rmu_sc0002680.1_g000029 Rmu_sc0002700.1_g000030 Rmu_sc0002700.1_g000031 Rmu_sc0002709.1_g000035 Rmu_sc0002779.1_g000017 Rmu_sc0003267.1_g000040 Rmu_sc0003306.1_g000009 Rmu_sc0003338.1_g000014 Rmu_sc0003522.1_g000038 Rmu_sc0003522.1_g000039 Rmu_sc0003653.1_g000031 Rmu_sc0003901.1_g000003 Rmu_sc0003920.1_g000009 Rmu_sc0004014.1_g000009 Rmu_sc0004016.1_g000013 Rmu_sc0004031.1_g000002 Rmu_sc0004106.1_g000001 Rmu_sc0004432.1_g000004 Rmu_sc0004481.1_g000020 Rmu_sc0004874.1_g000005 Rmu_sc0004928.1_g000005 Rmu_sc0005065.1_g000053 Rmu_sc0005091.1_g000005 Rmu_sc0005217.1_g000015 Rmu_sc0005242.1_g000002 Rmu_sc0005300.1_g000011 Rmu_sc0005400.1_g000004 Rmu_sc0005486.1_g000007 Rmu_sc0005527.1_g000015 Rmu_sc0006059.1_g000021 Rmu_sc0006087.1_g000004 Rmu_sc0006184.1_g000006 Rmu_sc0006184.1_g000007 Rmu_sc0006578.1_g000006 Rmu_sc0006730.1_g000010 Rmu_sc0006736.1_g000030 Rmu_sc0006736.1_g000033 Rmu_sc0007333.1_g000018 Rmu_sc0007856.1_g000010 Rmu_sc0008231.1_g000003 Rmu_sc0009862.1_g000012 Rmu_sc0010547.1_g000001 Rmu_sc0010547.1_g000003 Rmu_sc0010632.1_g000012 Rmu_sc0010747.1_g000008 Rmu_sc0011075.1_g000009 Rmu_sc0011565.1_g000004 Rmu_sc0011641.1_g000003 Rmu_sc0013102.1_g000013 Rmu_sc0013160.1_g000006 Rmu_sc0014846.1_g000004 Rmu_sc0017037.1_g000003 Rmu_sc0018203.1_g000001 Rmu_sc0018204.1_g000001 Rmu_sc0018348.1_g000001 Rmu_sc0019354.1_g000002 Rmu_sc0021630.1_g000002 Rmu_sc0022987.1_g000002 Rmu_sc0024469.1_g000005 Rmu_sc0033519.1_g000001 Rmu_sc0033519.1_g000002 Rmu_sc0033519.1_g000003 Rmu_sc0042775.1_g000001 Rmu_ssc0000110.1_g000003 Rmu_ssc0000110.1_g000004 Rmu_ssc0000126.1_g000068 Rmu_ssc0000200.1_g000007 Rmu_ssc0000295.1_g000003 Rmu_ssc0000295.1_g000004 Rmu_ssc0000299.1_g000010 Rmu_ssc0000308.1_g000024 Rmu_ssc0000339.1_g000006 Rmu_ssc0000385.1_g000012 Rmu_ssc0000409.1_g000004 Rmu_ssc0000421.1_g000004
rosa_roxburghii Rroxscaffold_156G00440360 Rroxscaffold_166G00440800 Rroxscaffold_1G00039040 Rroxscaffold_1G00039050 Rroxscaffold_1G00073950 Rroxscaffold_2G00091710 Rroxscaffold_2G00120050 Rroxscaffold_2G00122590 Rroxscaffold_3G00219700 Rroxscaffold_3G00264140 Rroxscaffold_4G00290600 Rroxscaffold_4G00323610 Rroxscaffold_4G00328460 Rroxscaffold_5G00343440 Rroxscaffold_5G00343570 Rroxscaffold_5G00378540 Rroxscaffold_5G00385090 Rroxscaffold_7G00169130 Rroxscaffold_7G00216420
rosa_rugosa Rorug01G0065800 Rorug01G0073500 Rorug01G0091700 Rorug01G0096500 Rorug01G0204700.1 Rorug01G0249100 Rorug01G0333800 Rorug01G0333900 Rorug01G0339500 Rorug01G0431300 Rorug02G0085400 Rorug02G0117500 Rorug02G0130900 Rorug02G0178800 Rorug02G0226700 Rorug02G0336300 Rorug02G0338700 Rorug02G0351400 Rorug02G0459900 Rorug03G0187000 Rorug03G0223700 Rorug03G0291300 Rorug03G0291300 Rorug03G0311100 Rorug03G0316900 Rorug03G0346000 Rorug04G0014500 Rorug04G0014600 Rorug04G0124100 Rorug04G0136500 Rorug04G0302000 Rorug04G0338100 Rorug04G0338200 Rorug04G0413100 Rorug05G0182200 Rorug05G0226500 Rorug05G0273300 Rorug05G0292200 Rorug05G0292200 Rorug05G0392500 Rorug05G0394500 Rorug05G0394600 Rorug05G0505600.1 Rorug06G0351400 Rorug06G0393400 Rorug06G0393600 Rorug06G0470000 Rorug07G0063100 Rorug07G0086800 Rorug07G0109200 Rorug07G0236100
rosa_samantha Rh1AG010800 Rh1AG011100 Rh1AG064900 Rh1AG088000 Rh1AG207100 Rh1AG262000 Rh1AG300800 Rh1BG043000 Rh1BG144600 Rh1BG180700 Rh1BG180800 Rh1BG328800 Rh1BG399400 Rh1CG041400 Rh1CG047300 Rh1CG379700 Rh1DG036500 Rh1DG083000 Rh1DG210700 Rh1DG210800 Rh1DG231100 Rh2AG007900 Rh2AG055500 Rh2AG339100 Rh2AG368100 Rh2BG243300 Rh2BG258900 Rh2BG292000 Rh2BG333500 Rh2BG387300 Rh2BG612800 Rh2BG619100 Rh2CG026500 Rh2CG163600 Rh2CG535700 Rh2DG186400 Rh2DG235000 Rh2DG314500 Rh2DG388500 Rh2DG391400 Rh2DG496900 Rh2DG526700 Rh2DG570700 Rh3AG330700 Rh3BG266900 Rh3CG236000 Rh3CG340400 Rh3DG090000 Rh3DG278400 Rh3DG297600 Rh3DG362600 Rh4AG001300 Rh4AG023300 Rh4AG097100 Rh4AG427000 Rh4BG029400 Rh4BG032400 Rh4BG086000 Rh4BG249200 Rh4CG001900 Rh4CG040600 Rh4CG093000 Rh4CG106300 Rh4CG113200 Rh4CG113900 Rh4DG002300 Rh4DG144400 Rh4DG234200 Rh5AG043700 Rh5AG084900 Rh5AG161500 Rh5AG409000 Rh5AG457200 Rh5AG457900 Rh5AG473700 Rh5AG520600 Rh5BG042100 Rh5BG124100 Rh5BG323500 Rh5BG417200 Rh5BG443700 Rh5BG451500 Rh5BG464900 Rh5BG470400 Rh5BG522500 Rh5CG068800 Rh5CG178300 Rh5CG231000 Rh5CG258600 Rh5CG394500 Rh5CG473700 Rh5DG164700 Rh5DG235400 Rh5DG261300 Rh5DG304900 Rh5DG334500 Rh5DG424300 Rh5DG458400 Rh5DG478600 Rh5DG552300 Rh5DG552700 Rh6AG037000 Rh6AG042100 Rh6AG255500 Rh6AG283400 Rh6AG291800 Rh6BG049600 Rh6BG080700 Rh6BG114600 Rh6BG128900 Rh6BG352500 Rh6CG052400 Rh6CG112900 Rh6CG113000 Rh6CG131300 Rh6CG157700 Rh6CG243200 Rh6CG254100 Rh6CG275700 Rh6CG299700 Rh6CG309100 Rh6CG317600 Rh6CG342600 Rh6CG471900 Rh6DG038200 Rh6DG102300 Rh6DG114400 Rh6DG225900 Rh6DG292900 Rh6DG320700 Rh6DG512800 Rh7AG217700 Rh7AG313900 Rh7AG446000 Rh7AG472600 Rh7BG223100 Rh7BG349900 Rh7BG352000 Rh7CG160200 Rh7CG233900 Rh7CG242700 Rh7CG273900 Rh7CG306400 Rh7CG322200 Rh7DG053800 Rh7DG077800 Rh7DG227700 Rh7DG404800
rosa_wichuraiana Rw0G003990 Rw2G022110 Rw2G024890 Rw6G005700 Rw7G008770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 2 cut(s) 587, 1571
AccBSI CCGCTC 1 cut(s) 391
AciI CCGC 6 cut(s) 391, 506, 874, 1163, 1402, 1638
AclI AACGTT 1 cut(s) 1393
AclWI GGATC 4 cut(s) 31, 335, 348, 1812
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 3 cut(s) 359, 1701, 1836
AcuI CTGAAG 1 cut(s) 1630
AdeI CACNNNGTG 2 cut(s) 262, 305
AfaI GTAC 5 cut(s) 317, 326, 383, 449, 589
AfiI CCNNNNNNNGG 5 cut(s) 147, 350, 584, 847, 1425
AgeI ACCGGT 1 cut(s) 564
AgsI TTSAA 9 cut(s) 47, 379, 551, 718, 825, 920, 1024, 1130, 1329
AjnI CCWGG 8 cut(s) 39, 336, 488, 577, 845, 1333, 1530, 1578
AluBI AGCT 7 cut(s) 119, 517, 533, 608, 1062, 1874, 1887
AluI AGCT 7 cut(s) 119, 517, 533, 608, 1062, 1874, 1887
Alw21I GWGCWC 1 cut(s) 91
Alw44I GTGCAC 1 cut(s) 87
AlwI GGATC 4 cut(s) 31, 335, 348, 1812
AlwNI CAGNNNCTG 1 cut(s) 1517
AoxI GGCC 7 cut(s) 467, 843, 938, 965, 1283, 1709, 1764
ApaI GGGCCC 1 cut(s) 969
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 5 cut(s) 764, 876, 1472, 1475, 1478
ApoI RAATTY 3 cut(s) 359, 1701, 1836
AseI ATTAAT 1 cut(s) 630
AsiGI ACCGGT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 417
Asp718I GGTACC 1 cut(s) 587
AspLEI GCGC 2 cut(s) 637, 947
AspS9I GGNCC 4 cut(s) 772, 965, 966, 1817
AsuC2I CCSGG 5 cut(s) 479, 842, 942, 964, 1346
AsuHPI GGTGA 6 cut(s) 18, 248, 572, 1090, 1333, 1880
AsuII TTCGAA 1 cut(s) 246
AvaII GGWCC 2 cut(s) 772, 1817
BaeGI GKGCMC 2 cut(s) 91, 969
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 587, 1571
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 3 cut(s) 206, 1023, 1636
Bbv12I GWGCWC 1 cut(s) 91
BbvI GCAGC 5 cut(s) 776, 863, 1459, 1462, 1490
BccI CCATC 7 cut(s) 261, 896, 993, 1310, 1381, 1435, 1769
BciT130I CCWGG 8 cut(s) 41, 338, 490, 579, 847, 1335, 1532, 1580
BciVI GTATCC 1 cut(s) 1729
BcnI CCSGG 5 cut(s) 479, 842, 942, 964, 1346
BfaI CTAG 5 cut(s) 6, 189, 1311, 1841, 1888
BfmI CTRYAG 1 cut(s) 1476
BfoI RGCGCY 1 cut(s) 948
BfuI GTATCC 1 cut(s) 1729
BisI GCNGC 6 cut(s) 392, 765, 877, 1473, 1476, 1479
BlpI GCTNAGC 1 cut(s) 518
BlsI GCNGC 6 cut(s) 393, 766, 878, 1474, 1477, 1480
Bme18I GGWCC 2 cut(s) 772, 1817
BmgT120I GGNCC 4 cut(s) 772, 965, 966, 1817
BmiI GGNNCC 7 cut(s) 342, 438, 589, 967, 968, 1573, 1720
BmsI GCATC 4 cut(s) 601, 1027, 1143, 1174
BpiI GAAGAC 3 cut(s) 206, 1023, 1636
BpmI CTGGAG 1 cut(s) 1012
Bpu10I CCTNAGC 2 cut(s) 112, 1287
Bpu1102I GCTNAGC 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 246
BpuMI CCSGG 5 cut(s) 479, 842, 942, 964, 1346
BsaBI GATNNNNATC 1 cut(s) 1368
BsaJI CCNNGG 6 cut(s) 279, 577, 846, 887, 1344, 1459
BsaWI WCCGGW 1 cut(s) 564
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 350, 584, 847, 1425
Bse118I RCCGGY 2 cut(s) 433, 564
Bse1I ACTGG 7 cut(s) 775, 956, 983, 995, 1493, 1865, 1870
Bse3DI GCAATG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 1368
BseBI CCWGG 8 cut(s) 41, 338, 490, 579, 847, 1335, 1532, 1580
BseDI CCNNGG 6 cut(s) 279, 577, 846, 887, 1344, 1459
BseGI GGATG 5 cut(s) 1004, 1302, 1392, 1626, 1785
BseJI GATNNNNATC 1 cut(s) 1368
BseLI CCNNNNNNNGG 5 cut(s) 147, 350, 584, 847, 1425
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 3 cut(s) 485, 1278, 1697
BseNI ACTGG 7 cut(s) 775, 956, 983, 995, 1493, 1865, 1870
BseRI GAGGAG 1 cut(s) 807
BseSI GKGCMC 2 cut(s) 91, 969
BseXI GCAGC 5 cut(s) 776, 863, 1459, 1462, 1490
BshFI GGCC 7 cut(s) 469, 845, 940, 967, 1285, 1711, 1766
BshNI GGYRCC 2 cut(s) 587, 1571
BshTI ACCGGT 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 7 cut(s) 434, 478, 565, 842, 941, 963, 1346
BslFI GGGAC 2 cut(s) 23, 1214
BslI CCNNNNNNNGG 5 cut(s) 147, 350, 584, 847, 1425
BsmFI GGGAC 2 cut(s) 23, 1214
BsmI GAATGC 2 cut(s) 1136, 1456
BsnI GGCC 7 cut(s) 469, 845, 940, 967, 1285, 1711, 1766
Bsp119I TTCGAA 1 cut(s) 246
Bsp120I GGGCCC 1 cut(s) 965
Bsp1286I GDGCHC 2 cut(s) 91, 969
Bsp143I GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
Bsp1720I GCTNAGC 1 cut(s) 518
BspACI CCGC 6 cut(s) 391, 506, 874, 1163, 1402, 1638
BspANI GGCC 7 cut(s) 469, 845, 940, 967, 1285, 1711, 1766
BspCNI CTCAG 3 cut(s) 484, 1279, 1698
BspHI TCATGA 1 cut(s) 1807
BspLI GGNNCC 7 cut(s) 342, 438, 589, 967, 968, 1573, 1720
BspMAI CTGCAG 1 cut(s) 1480
BspPI GGATC 4 cut(s) 31, 335, 348, 1812
BspQI GCTCTTC 1 cut(s) 1052
BspT104I TTCGAA 1 cut(s) 246
BspT107I GGYRCC 2 cut(s) 587, 1571
BsrBI CCGCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 2 cut(s) 433, 564
BsrI ACTGG 7 cut(s) 775, 956, 983, 995, 1493, 1865, 1870
BssAI RCCGGY 2 cut(s) 433, 564
BssECI CCNNGG 6 cut(s) 279, 577, 846, 887, 1344, 1459
BssMI GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
Bst2UI CCWGG 8 cut(s) 41, 338, 490, 579, 847, 1335, 1532, 1580
Bst4CI ACNGT 7 cut(s) 303, 320, 869, 888, 1513, 1723, 1741
Bst6I CTCTTC 4 cut(s) 53, 713, 1052, 1784
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 762, 1293, 1636
BstDEI CTNAG 7 cut(s) 112, 471, 518, 651, 935, 1287, 1706
BstDSI CCRYGG 1 cut(s) 887
BstEII GGTNACC 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 1004, 1302, 1392, 1626, 1785
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 2 cut(s) 637, 947
BstKTI GATC 7 cut(s) 26, 75, 343, 1096, 1372, 1624, 1807
BstMBI GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
BstMWI GCNNNNNNNGC 5 cut(s) 514, 1046, 1140, 1268, 1478
BstNI CCWGG 8 cut(s) 41, 338, 490, 579, 847, 1335, 1532, 1580
BstNSI RCATGY 1 cut(s) 764
BstPI GGTNACC 1 cut(s) 560
BstSFI CTRYAG 1 cut(s) 1476
BstSLI GKGCMC 2 cut(s) 91, 969
BstV1I GCAGC 5 cut(s) 776, 863, 1459, 1462, 1490
BstV2I GAAGAC 3 cut(s) 206, 1023, 1636
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuI GTATCC 1 cut(s) 1729
BsuRI GGCC 7 cut(s) 469, 845, 940, 967, 1285, 1711, 1766
BtgI CCRYGG 1 cut(s) 887
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 5 cut(s) 1004, 1302, 1392, 1626, 1785
BtsI GCAGTG 1 cut(s) 1327
BtsIMutI CAGTG 4 cut(s) 325, 865, 988, 1327
Cac8I GCNNGC 3 cut(s) 762, 1293, 1636
CaiI CAGNNNCTG 1 cut(s) 1517
CciI TCATGA 1 cut(s) 1807
CfoI GCGC 2 cut(s) 637, 947
Cfr10I RCCGGY 2 cut(s) 433, 564
Cfr13I GGNCC 4 cut(s) 772, 965, 966, 1817
CseI GACGC 1 cut(s) 1470
CsiI ACCWGGT 1 cut(s) 39
Csp6I GTAC 5 cut(s) 316, 325, 382, 448, 588
CspAI ACCGGT 1 cut(s) 564
CviAII CATG 7 cut(s) 310, 592, 761, 817, 1447, 1631, 1808
CviQI GTAC 5 cut(s) 316, 325, 382, 448, 588
DdeI CTNAG 7 cut(s) 112, 471, 518, 651, 935, 1287, 1706
DpnI GATC 7 cut(s) 25, 74, 342, 1095, 1371, 1623, 1806
DpnII GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
DraIII CACNNNGTG 2 cut(s) 262, 305
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 4 cut(s) 53, 713, 1052, 1784
EarI CTCTTC 4 cut(s) 53, 713, 1052, 1784
Eco24I GRGCYC 1 cut(s) 969
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 772, 1817
Eco57I CTGAAG 1 cut(s) 1630
Eco91I GGTNACC 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 966
EcoO65I GGTNACC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 1701
EcoRII CCWGG 8 cut(s) 39, 336, 488, 577, 845, 1333, 1530, 1578
EcoRV GATATC 1 cut(s) 268
EcoT22I ATGCAT 1 cut(s) 1136
EcoT38I GRGCYC 1 cut(s) 969
FaeI CATG 7 cut(s) 313, 595, 764, 820, 1450, 1634, 1811
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 2 cut(s) 23, 1214
FatI CATG 7 cut(s) 309, 591, 760, 816, 1446, 1630, 1807
FauI CCCGC 1 cut(s) 881
Fnu4HI GCNGC 6 cut(s) 392, 765, 877, 1473, 1476, 1479
FokI GGATG 5 cut(s) 1011, 1289, 1399, 1613, 1792
FriOI GRGCYC 1 cut(s) 969
Fsp4HI GCNGC 6 cut(s) 392, 765, 877, 1473, 1476, 1479
FspBI CTAG 5 cut(s) 6, 189, 1311, 1841, 1888
GlaI GCGC 2 cut(s) 636, 946
GluI GCNGC 6 cut(s) 392, 765, 877, 1473, 1476, 1479
GsuI CTGGAG 1 cut(s) 1012
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 7 cut(s) 469, 845, 940, 967, 1285, 1711, 1766
HapII CCGG 7 cut(s) 434, 478, 565, 842, 941, 963, 1346
HgaI GACGC 1 cut(s) 1470
HhaI GCGC 2 cut(s) 637, 947
Hin1II CATG 7 cut(s) 313, 595, 764, 820, 1450, 1634, 1811
Hin6I GCGC 2 cut(s) 635, 945
HinP1I GCGC 2 cut(s) 635, 945
HpaII CCGG 7 cut(s) 434, 478, 565, 842, 941, 963, 1346
HphI GGTGA 6 cut(s) 18, 248, 572, 1090, 1333, 1880
Hpy166II GTNNAC 4 cut(s) 89, 327, 925, 1817
Hpy188III TCNNGA 7 cut(s) 289, 730, 1105, 1187, 1658, 1808, 1829
Hpy8I GTNNAC 4 cut(s) 89, 327, 925, 1817
Hpy99I CGWCG 1 cut(s) 1486
HpyAV CCTTC 3 cut(s) 1069, 1419, 1709
HpyCH4III ACNGT 7 cut(s) 303, 320, 869, 888, 1513, 1723, 1741
HpyCH4IV ACGT 3 cut(s) 314, 1257, 1393
HpyF10VI GCNNNNNNNGC 5 cut(s) 514, 1046, 1140, 1268, 1478
HpyF3I CTNAG 7 cut(s) 112, 471, 518, 651, 935, 1287, 1706
HpySE526I ACGT 3 cut(s) 314, 1257, 1393
Hsp92II CATG 7 cut(s) 313, 595, 764, 820, 1450, 1634, 1811
HspAI GCGC 2 cut(s) 635, 945
KpnI GGTACC 1 cut(s) 591
Kzo9I GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
LguI GCTCTTC 1 cut(s) 1052
LmnI GCTCC 6 cut(s) 116, 142, 442, 514, 605, 751
Lsp1109I GCAGC 5 cut(s) 776, 863, 1459, 1462, 1490
LweI GCATC 4 cut(s) 601, 1027, 1143, 1174
MabI ACCWGGT 1 cut(s) 39
MaeI CTAG 5 cut(s) 6, 189, 1311, 1841, 1888
MaeII ACGT 3 cut(s) 314, 1257, 1393
MaeIII GTNAC 4 cut(s) 104, 560, 1394, 1667
MalI GATC 7 cut(s) 25, 74, 342, 1095, 1371, 1623, 1806
MbiI CCGCTC 1 cut(s) 391
MboI GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 91, 969
MluCI AATT 8 cut(s) 359, 541, 672, 915, 1413, 1701, 1797, 1836
MlyI GAGTC 3 cut(s) 230, 448, 1801
Mph1103I ATGCAT 1 cut(s) 1136
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 5 cut(s) 630, 975, 1155, 1356, 1824
MslI CAYNNNNRTG 1 cut(s) 1812
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 7 cut(s) 434, 478, 565, 842, 941, 963, 1346
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 2 cut(s) 1136, 1456
MvaI CCWGG 8 cut(s) 41, 338, 490, 579, 847, 1335, 1532, 1580
MwoI GCNNNNNNNGC 5 cut(s) 514, 1046, 1140, 1268, 1478
NciI CCSGG 5 cut(s) 479, 842, 942, 964, 1346
NdeII GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
NlaIII CATG 7 cut(s) 313, 595, 764, 820, 1450, 1634, 1811
NlaIV GGNNCC 7 cut(s) 342, 438, 589, 967, 968, 1573, 1720
NmuCI GTSAC 2 cut(s) 560, 1667
NsiI ATGCAT 1 cut(s) 1136
NspI RCATGY 1 cut(s) 764
NspV TTCGAA 1 cut(s) 246
PaeI GCATGC 1 cut(s) 764
PagI TCATGA 1 cut(s) 1807
PciSI GCTCTTC 1 cut(s) 1052
PctI GAATGC 2 cut(s) 1136, 1456
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 7 cut(s) 47, 547, 573, 955, 1081, 1314, 1338
PflFI GACNNNGTC 1 cut(s) 736
PinAI ACCGGT 1 cut(s) 564
PkrI GCNGC 6 cut(s) 393, 766, 878, 1474, 1477, 1480
PleI GAGTC 3 cut(s) 229, 448, 1800
PpsI GAGTC 3 cut(s) 229, 448, 1800
PshBI ATTAAT 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 1393
Psp6I CCWGG 8 cut(s) 39, 336, 488, 577, 845, 1333, 1530, 1578
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 8 cut(s) 39, 336, 488, 577, 845, 1333, 1530, 1578
PspN4I GGNNCC 7 cut(s) 342, 438, 589, 967, 968, 1573, 1720
PspOMI GGGCCC 1 cut(s) 965
PspPI GGNCC 4 cut(s) 772, 965, 966, 1817
PstI CTGCAG 1 cut(s) 1480
PstNI CAGNNNCTG 1 cut(s) 1517
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 736
RsaI GTAC 5 cut(s) 317, 326, 383, 449, 589
RsaNI GTAC 5 cut(s) 316, 325, 382, 448, 588
RseI CAYNNNNRTG 1 cut(s) 1812
SapI GCTCTTC 1 cut(s) 1052
SaqAI TTAA 5 cut(s) 630, 975, 1155, 1356, 1824
SatI GCNGC 6 cut(s) 392, 765, 877, 1473, 1476, 1479
Sau3AI GATC 7 cut(s) 23, 72, 340, 1093, 1369, 1621, 1804
Sau96I GGNCC 4 cut(s) 772, 965, 966, 1817
SchI GAGTC 3 cut(s) 230, 448, 1801
SduI GDGCHC 2 cut(s) 91, 969
SexAI ACCWGGT 1 cut(s) 39
SfaNI GCATC 4 cut(s) 601, 1027, 1143, 1174
SfcI CTRYAG 1 cut(s) 1476
SfuI TTCGAA 1 cut(s) 246
SinI GGWCC 2 cut(s) 772, 1817
SmiMI CAYNNNNRTG 1 cut(s) 1812
SphI GCATGC 1 cut(s) 764
Sse9I AATT 8 cut(s) 359, 541, 672, 915, 1413, 1701, 1797, 1836
SsiI CCGC 6 cut(s) 391, 506, 874, 1163, 1402, 1638
SspMI CTAG 5 cut(s) 6, 189, 1311, 1841, 1888
TaaI ACNGT 7 cut(s) 303, 320, 869, 888, 1513, 1723, 1741
TaiI ACGT 3 cut(s) 317, 1260, 1396
TaqI TCGA 5 cut(s) 246, 363, 855, 1484, 1601
TaqII GACCGA 1 cut(s) 138
TasI AATT 8 cut(s) 359, 541, 672, 915, 1413, 1701, 1797, 1836
TatI WGTACW 3 cut(s) 324, 381, 447
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 7 cut(s) 47, 547, 573, 955, 1081, 1314, 1338
Tru1I TTAA 5 cut(s) 630, 975, 1155, 1356, 1824
Tru9I TTAA 5 cut(s) 630, 975, 1155, 1356, 1824
TscAI CASTG 4 cut(s) 325, 872, 995, 1327
TseFI GTSAC 2 cut(s) 560, 1667
TseI GCWGC 5 cut(s) 764, 876, 1472, 1475, 1478
Tsp45I GTSAC 2 cut(s) 560, 1667
TspDTI ATGAA 6 cut(s) 195, 212, 1463, 1520, 1555, 1569
TspRI CASTG 4 cut(s) 325, 872, 995, 1327
Tth111I GACNNNGTC 1 cut(s) 736
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 772, 1817
VspI ATTAAT 1 cut(s) 630
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 3 cut(s) 359, 1701, 1836
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 2 cut(s) 1194, 1538
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 5 cut(s) 6, 189, 1311, 1841, 1888
Zsp2I ATGCAT 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.