Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2660 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 717 |
| Acc16I | TGCGCA | 1 cut(s) | 951 |
| Acc36I | ACCTGC | 1 cut(s) | 2210 |
| AccB1I | GGYRCC | 2 cut(s) | 1157, 1931 |
| AccBSI | CCGCTC | 2 cut(s) | 816, 2017 |
| AccII | CGCG | 2 cut(s) | 159, 847 |
| AclI | AACGTT | 1 cut(s) | 2247 |
| AclWI | GGATC | 2 cut(s) | 1553, 1566 |
| AcoI | YGGCCR | 1 cut(s) | 2296 |
| AcsI | RAATTY | 2 cut(s) | 370, 1175 |
| AcuI | CTGAAG | 3 cut(s) | 90, 1584, 1735 |
| AcyI | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| AdeI | CACNNNGTG | 1 cut(s) | 521 |
| AfaI | GTAC | 7 cut(s) | 54, 431, 459, 930, 1255, 1970, 2398 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 212, 889, 1168, 1487, 2305, 2609, 2610 |
| AflII | CTTAAG | 1 cut(s) | 1682 |
| AgeI | ACCGGT | 1 cut(s) | 973 |
| AhlI | ACTAGT | 2 cut(s) | 2426, 2453 |
| AjnI | CCWGG | 7 cut(s) | 548, 1036, 1092, 1249, 1578, 1639, 2644 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1658, 1690, 1862, 1894 |
| AleI | CACNNNNGTG | 1 cut(s) | 2429 |
| Alw21I | GWGCWC | 2 cut(s) | 591, 696 |
| AlwI | GGATC | 2 cut(s) | 1553, 1566 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1585, 1793 |
| Ama87I | CYCGRG | 1 cut(s) | 1837 |
| ApaI | GGGCCC | 1 cut(s) | 2368 |
| ApoI | RAATTY | 2 cut(s) | 370, 1175 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2039, 2071 |
| AseI | ATTAAT | 1 cut(s) | 1629 |
| AsiGI | ACCGGT | 1 cut(s) | 973 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1179 |
| AspA2I | CCTAGG | 1 cut(s) | 2552 |
| AspLEI | GCGC | 8 cut(s) | 159, 244, 348, 849, 864, 952, 1160, 1716 |
| AsuC2I | CCSGG | 6 cut(s) | 381, 797, 1321, 1838, 1839, 2036 |
| AsuHPI | GGTGA | 4 cut(s) | 20, 411, 946, 2605 |
| AsuNHI | GCTAGC | 2 cut(s) | 238, 1010 |
| AvaI | CYCGRG | 1 cut(s) | 1837 |
| AvaII | GGWCC | 6 cut(s) | 490, 1267, 1334, 1601, 1643, 2210 |
| AvrII | CCTAGG | 1 cut(s) | 2552 |
| BaeGI | GKGCMC | 2 cut(s) | 2368, 2604 |
| BamHI | GGATCC | 1 cut(s) | 1558 |
| BanI | GGYRCC | 2 cut(s) | 1157, 1931 |
| BanII | GRGCYC | 1 cut(s) | 2368 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 180, 212 |
| BbsI | GAAGAC | 1 cut(s) | 1706 |
| Bbv12I | GWGCWC | 2 cut(s) | 591, 696 |
| BccI | CCATC | 5 cut(s) | 110, 1325, 1463, 1538, 2484 |
| BceAI | ACGGC | 1 cut(s) | 837 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1286, 1320 |
| BciT130I | CCWGG | 7 cut(s) | 550, 1038, 1094, 1251, 1580, 1641, 2646 |
| BciVI | GTATCC | 1 cut(s) | 910 |
| BclI | TGATCA | 4 cut(s) | 396, 622, 1072, 1719 |
| BcnI | CCSGG | 6 cut(s) | 381, 797, 1321, 1838, 1839, 2036 |
| BcuI | ACTAGT | 2 cut(s) | 2426, 2453 |
| BfmI | CTRYAG | 2 cut(s) | 1271, 1586 |
| BfoI | RGCGCY | 1 cut(s) | 1161 |
| BfrI | CTTAAG | 1 cut(s) | 1682 |
| BfuAI | ACCTGC | 1 cut(s) | 2210 |
| BfuI | GTATCC | 1 cut(s) | 910 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1410 |
| BglII | AGATCT | 1 cut(s) | 563 |
| BlnI | CCTAGG | 1 cut(s) | 2552 |
| Bme18I | GGWCC | 6 cut(s) | 490, 1267, 1334, 1601, 1643, 2210 |
| BmeT110I | CYCGRG | 1 cut(s) | 1837 |
| BmrI | ACTGGG | 2 cut(s) | 83, 1602 |
| BmsI | GCATC | 3 cut(s) | 883, 1606, 2400 |
| BmtI | GCTAGC | 2 cut(s) | 242, 1014 |
| BmuI | ACTGGG | 2 cut(s) | 83, 1602 |
| BpiI | GAAGAC | 1 cut(s) | 1706 |
| BpmI | CTGGAG | 3 cut(s) | 1082, 1164, 2082 |
| Bpu10I | CCTNAGC | 1 cut(s) | 666 |
| BpuMI | CCSGG | 6 cut(s) | 381, 797, 1321, 1838, 1839, 2036 |
| BsaAI | YACGTR | 1 cut(s) | 1918 |
| BsaBI | GATNNNNATC | 1 cut(s) | 600 |
| BsaHI | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| BsaJI | CCNNGG | 8 cut(s) | 380, 796, 1639, 1836, 1837, 2206, 2552, 2644 |
| BsaWI | WCCGGW | 1 cut(s) | 973 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 212, 889, 1168, 1487, 2305, 2609, 2610 |
| Bse118I | RCCGGY | 3 cut(s) | 973, 1264, 1367 |
| Bse1I | ACTGG | 7 cut(s) | 78, 546, 689, 918, 1184, 1597, 1927 |
| Bse3DI | GCAATG | 3 cut(s) | 1755, 1830, 2398 |
| Bse8I | GATNNNNATC | 1 cut(s) | 600 |
| BseBI | CCWGG | 7 cut(s) | 550, 1038, 1094, 1251, 1580, 1641, 2646 |
| BseDI | CCNNGG | 8 cut(s) | 380, 796, 1639, 1836, 1837, 2206, 2552, 2644 |
| BseGI | GGATG | 3 cut(s) | 121, 874, 2476 |
| BseJI | GATNNNNATC | 1 cut(s) | 600 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 212, 889, 1168, 1487, 2305, 2609, 2610 |
| BseMI | GCAATG | 3 cut(s) | 1755, 1830, 2398 |
| BseNI | ACTGG | 7 cut(s) | 78, 546, 689, 918, 1184, 1597, 1927 |
| BseRI | GAGGAG | 2 cut(s) | 158, 741 |
| BseSI | GKGCMC | 2 cut(s) | 2368, 2604 |
| BseYI | CCCAGC | 3 cut(s) | 1694, 2107, 2367 |
| BsgI | GTGCAG | 1 cut(s) | 810 |
| Bsh1236I | CGCG | 2 cut(s) | 159, 847 |
| Bsh1285I | CGRYCG | 1 cut(s) | 845 |
| BshNI | GGYRCC | 2 cut(s) | 1157, 1931 |
| BshTI | ACCGGT | 1 cut(s) | 973 |
| BsiEI | CGRYCG | 1 cut(s) | 845 |
| BsiHKAI | GWGCWC | 2 cut(s) | 591, 696 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1837 |
| BsiSI | CCGG | 9 cut(s) | 380, 796, 974, 1265, 1320, 1337, 1368, 1838, 2036 |
| BslFI | GGGAC | 6 cut(s) | 503, 665, 813, 1016, 1854, 2102 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 212, 889, 1168, 1487, 2305, 2609, 2610 |
| BsmFI | GGGAC | 6 cut(s) | 503, 665, 813, 1016, 1854, 2102 |
| BsoBI | CYCGRG | 1 cut(s) | 1837 |
| Bsp120I | GGGCCC | 1 cut(s) | 2364 |
| Bsp1286I | GDGCHC | 4 cut(s) | 591, 696, 2368, 2604 |
| BspFNI | CGCG | 2 cut(s) | 159, 847 |
| BspHI | TCATGA | 1 cut(s) | 1899 |
| BspMAI | CTGCAG | 1 cut(s) | 1590 |
| BspMI | ACCTGC | 1 cut(s) | 2210 |
| BspOI | GCTAGC | 2 cut(s) | 242, 1014 |
| BspPI | GGATC | 2 cut(s) | 1553, 1566 |
| BspQI | GCTCTTC | 2 cut(s) | 1021, 1116 |
| BspT107I | GGYRCC | 2 cut(s) | 1157, 1931 |
| BspTI | CTTAAG | 1 cut(s) | 1682 |
| BsrBI | CCGCTC | 2 cut(s) | 816, 2017 |
| BsrDI | GCAATG | 3 cut(s) | 1755, 1830, 2398 |
| BsrFI | RCCGGY | 3 cut(s) | 973, 1264, 1367 |
| BsrI | ACTGG | 7 cut(s) | 78, 546, 689, 918, 1184, 1597, 1927 |
| BssAI | RCCGGY | 3 cut(s) | 973, 1264, 1367 |
| BssECI | CCNNGG | 8 cut(s) | 380, 796, 1639, 1836, 1837, 2206, 2552, 2644 |
| BssNI | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| BssT1I | CCWWGG | 2 cut(s) | 2206, 2552 |
| Bst2UI | CCWGG | 7 cut(s) | 550, 1038, 1094, 1251, 1580, 1641, 2646 |
| Bst4CI | ACNGT | 5 cut(s) | 8, 130, 871, 1951, 2593 |
| Bst6I | CTCTTC | 4 cut(s) | 1021, 1116, 1161, 2487 |
| BstACI | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| BstAFI | CTTAAG | 1 cut(s) | 1682 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 250 |
| BstBAI | YACGTR | 1 cut(s) | 1918 |
| BstDEI | CTNAG | 3 cut(s) | 666, 1080, 1445 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1166 |
| BstF5I | GGATG | 3 cut(s) | 121, 874, 2476 |
| BstFNI | CGCG | 2 cut(s) | 159, 847 |
| BstH2I | RGCGCY | 1 cut(s) | 1161 |
| BstHHI | GCGC | 8 cut(s) | 159, 244, 348, 849, 864, 952, 1160, 1716 |
| BstMCI | CGRYCG | 1 cut(s) | 845 |
| BstNI | CCWGG | 7 cut(s) | 550, 1038, 1094, 1251, 1580, 1641, 2646 |
| BstNSI | RCATGY | 1 cut(s) | 1430 |
| BstSFI | CTRYAG | 2 cut(s) | 1271, 1586 |
| BstSLI | GKGCMC | 2 cut(s) | 2368, 2604 |
| BstUI | CGCG | 2 cut(s) | 159, 847 |
| BstV2I | GAAGAC | 1 cut(s) | 1706 |
| BstX2I | RGATCY | 2 cut(s) | 563, 1558 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2294 |
| BstYI | RGATCY | 2 cut(s) | 563, 1558 |
| BsuI | GTATCC | 1 cut(s) | 910 |
| BtsCI | GGATG | 3 cut(s) | 121, 874, 2476 |
| BtsI | GCAGTG | 1 cut(s) | 2268 |
| BtsIMutI | CAGTG | 7 cut(s) | 71, 696, 876, 911, 1730, 1947, 2268 |
| BveI | ACCTGC | 1 cut(s) | 2210 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1585, 1793 |
| CciI | TCATGA | 1 cut(s) | 1899 |
| CfoI | GCGC | 8 cut(s) | 159, 244, 348, 849, 864, 952, 1160, 1716 |
| Cfr10I | RCCGGY | 3 cut(s) | 973, 1264, 1367 |
| Cfr9I | CCCGGG | 1 cut(s) | 1837 |
| CseI | GACGC | 5 cut(s) | 109, 554, 662, 810, 1799 |
| CsiI | ACCWGGT | 2 cut(s) | 1036, 1249 |
| Csp6I | GTAC | 7 cut(s) | 53, 430, 458, 929, 1254, 1969, 2397 |
| CspAI | ACCGGT | 1 cut(s) | 973 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1304, 1339 |
| CviAII | CATG | 9 cut(s) | 233, 313, 1298, 1427, 1440, 1900, 2172, 2224, 2492 |
| CviQI | GTAC | 7 cut(s) | 53, 430, 458, 929, 1254, 1969, 2397 |
| DdeI | CTNAG | 3 cut(s) | 666, 1080, 1445 |
| DinI | GGCGCC | 1 cut(s) | 1159 |
| DraI | TTTAAA | 1 cut(s) | 375 |
| DraIII | CACNNNGTG | 1 cut(s) | 521 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 717 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 717 |
| EaeI | YGGCCR | 1 cut(s) | 2296 |
| Eam1104I | CTCTTC | 4 cut(s) | 1021, 1116, 1161, 2487 |
| EarI | CTCTTC | 4 cut(s) | 1021, 1116, 1161, 2487 |
| EciI | GGCGGA | 1 cut(s) | 897 |
| Eco130I | CCWWGG | 2 cut(s) | 2206, 2552 |
| Eco24I | GRGCYC | 1 cut(s) | 2368 |
| Eco47I | GGWCC | 6 cut(s) | 490, 1267, 1334, 1601, 1643, 2210 |
| Eco57I | CTGAAG | 3 cut(s) | 90, 1584, 1735 |
| Eco88I | CYCGRG | 1 cut(s) | 1837 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1166 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1582, 1643, 2210, 2364 |
| EcoRII | CCWGG | 7 cut(s) | 548, 1036, 1092, 1249, 1578, 1639, 2644 |
| EcoT14I | CCWWGG | 2 cut(s) | 2206, 2552 |
| EcoT38I | GRGCYC | 1 cut(s) | 2368 |
| EgeI | GGCGCC | 1 cut(s) | 1159 |
| EheI | GGCGCC | 1 cut(s) | 1159 |
| ErhI | CCWWGG | 2 cut(s) | 2206, 2552 |
| FaeI | CATG | 9 cut(s) | 236, 316, 1301, 1430, 1443, 1903, 2175, 2227, 2495 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1862, 1894 |
| FaqI | GGGAC | 6 cut(s) | 503, 665, 813, 1016, 1854, 2102 |
| FatI | CATG | 9 cut(s) | 232, 312, 1297, 1426, 1439, 1899, 2171, 2223, 2491 |
| FauI | CCCGC | 3 cut(s) | 821, 886, 2127 |
| FbaI | TGATCA | 4 cut(s) | 396, 622, 1072, 1719 |
| FokI | GGATG | 3 cut(s) | 128, 861, 2463 |
| FriOI | GRGCYC | 1 cut(s) | 2368 |
| FspI | TGCGCA | 1 cut(s) | 951 |
| GlaI | GCGC | 8 cut(s) | 158, 243, 347, 848, 863, 951, 1159, 1715 |
| GsaI | CCCAGC | 3 cut(s) | 1698, 2111, 2371 |
| GsuI | CTGGAG | 3 cut(s) | 1082, 1164, 2082 |
| HaeII | RGCGCY | 1 cut(s) | 1161 |
| HapII | CCGG | 9 cut(s) | 380, 796, 974, 1265, 1320, 1337, 1368, 1838, 2036 |
| HgaI | GACGC | 5 cut(s) | 109, 554, 662, 810, 1799 |
| HhaI | GCGC | 8 cut(s) | 159, 244, 348, 849, 864, 952, 1160, 1716 |
| Hin1I | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| Hin1II | CATG | 9 cut(s) | 236, 316, 1301, 1430, 1443, 1903, 2175, 2227, 2495 |
| Hin6I | GCGC | 8 cut(s) | 157, 242, 346, 847, 862, 950, 1158, 1714 |
| HinP1I | GCGC | 8 cut(s) | 157, 242, 346, 847, 862, 950, 1158, 1714 |
| HincII | GTYRAC | 3 cut(s) | 991, 2131, 2572 |
| HindII | GTYRAC | 3 cut(s) | 991, 2131, 2572 |
| HindIII | AAGCTT | 2 cut(s) | 1208, 1679 |
| HinfI | GANTC | 7 cut(s) | 67, 680, 764, 779, 899, 1303, 2496 |
| HpaI | GTTAAC | 1 cut(s) | 991 |
| HpaII | CCGG | 9 cut(s) | 380, 796, 974, 1265, 1320, 1337, 1368, 1838, 2036 |
| HphI | GGTGA | 4 cut(s) | 20, 411, 946, 2605 |
| Hpy166II | GTNNAC | 5 cut(s) | 710, 793, 991, 2131, 2572 |
| Hpy188I | TCNGA | 5 cut(s) | 210, 810, 1225, 1615, 2331 |
| Hpy188III | TCNNGA | 5 cut(s) | 1143, 1562, 1900, 1964, 2099 |
| Hpy8I | GTNNAC | 5 cut(s) | 710, 793, 991, 2131, 2572 |
| Hpy99I | CGWCG | 3 cut(s) | 125, 430, 1742 |
| HpyAV | CCTTC | 3 cut(s) | 114, 1718, 2349 |
| HpyCH4III | ACNGT | 5 cut(s) | 8, 130, 871, 1951, 2593 |
| HpyCH4IV | ACGT | 7 cut(s) | 413, 428, 460, 999, 1917, 2115, 2247 |
| HpyF3I | CTNAG | 3 cut(s) | 666, 1080, 1445 |
| HpySE526I | ACGT | 7 cut(s) | 413, 428, 460, 999, 1917, 2115, 2247 |
| Hsp92I | GRCGYC | 3 cut(s) | 120, 546, 1158 |
| Hsp92II | CATG | 9 cut(s) | 236, 316, 1301, 1430, 1443, 1903, 2175, 2227, 2495 |
| HspAI | GCGC | 8 cut(s) | 157, 242, 346, 847, 862, 950, 1158, 1714 |
| KasI | GGCGCC | 1 cut(s) | 1157 |
| Ksp22I | TGATCA | 4 cut(s) | 396, 622, 1072, 1719 |
| KspAI | GTTAAC | 1 cut(s) | 991 |
| LguI | GCTCTTC | 2 cut(s) | 1021, 1116 |
| LmnI | GCTCC | 4 cut(s) | 145, 821, 910, 2022 |
| LweI | GCATC | 3 cut(s) | 883, 1606, 2400 |
| MabI | ACCWGGT | 2 cut(s) | 1036, 1249 |
| MaeII | ACGT | 7 cut(s) | 413, 428, 460, 999, 1917, 2115, 2247 |
| MaeIII | GTNAC | 4 cut(s) | 124, 658, 719, 1918 |
| MbiI | CCGCTC | 2 cut(s) | 816, 2017 |
| MflI | RGATCY | 2 cut(s) | 563, 1558 |
| MhlI | GDGCHC | 4 cut(s) | 591, 696, 2368, 2604 |
| Mly113I | GGCGCC | 1 cut(s) | 1158 |
| MlyI | GAGTC | 2 cut(s) | 689, 908 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1179 |
| MseI | TTAA | 9 cut(s) | 374, 990, 1469, 1629, 1683, 1803, 2090, 2352, 2421 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2429, 2490 |
| MspA1I | CMGCKG | 3 cut(s) | 841, 1088, 2371 |
| MspCI | CTTAAG | 1 cut(s) | 1682 |
| MspI | CCGG | 9 cut(s) | 380, 796, 974, 1265, 1320, 1337, 1368, 1838, 2036 |
| MvaI | CCWGG | 7 cut(s) | 550, 1038, 1094, 1251, 1580, 1641, 2646 |
| MvnI | CGCG | 2 cut(s) | 159, 847 |
| NarI | GGCGCC | 1 cut(s) | 1158 |
| NciI | CCSGG | 6 cut(s) | 381, 797, 1321, 1838, 1839, 2036 |
| NheI | GCTAGC | 2 cut(s) | 238, 1010 |
| NlaIII | CATG | 9 cut(s) | 236, 316, 1301, 1430, 1443, 1903, 2175, 2227, 2495 |
| NmuCI | GTSAC | 3 cut(s) | 124, 719, 1918 |
| NsbI | TGCGCA | 1 cut(s) | 951 |
| NspI | RCATGY | 1 cut(s) | 1430 |
| OliI | CACNNNNGTG | 1 cut(s) | 2429 |
| PaeI | GCATGC | 1 cut(s) | 1430 |
| PagI | TCATGA | 1 cut(s) | 1899 |
| PciSI | GCTCTTC | 2 cut(s) | 1021, 1116 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1179 |
| PfeI | GAWTC | 5 cut(s) | 67, 764, 779, 1303, 2496 |
| PinAI | ACCGGT | 1 cut(s) | 973 |
| PleI | GAGTC | 2 cut(s) | 688, 907 |
| PluTI | GGCGCC | 1 cut(s) | 1161 |
| PpsI | GAGTC | 2 cut(s) | 688, 907 |
| Ppu21I | YACGTR | 1 cut(s) | 1918 |
| PpuMI | RGGWCCY | 2 cut(s) | 1643, 2210 |
| PshBI | ATTAAT | 1 cut(s) | 1629 |
| PsiI | TTATAA | 1 cut(s) | 2660 |
| Psp1406I | AACGTT | 1 cut(s) | 2247 |
| Psp5II | RGGWCCY | 2 cut(s) | 1643, 2210 |
| Psp6I | CCWGG | 7 cut(s) | 548, 1036, 1092, 1249, 1578, 1639, 2644 |
| PspFI | CCCAGC | 3 cut(s) | 1694, 2107, 2367 |
| PspGI | CCWGG | 7 cut(s) | 548, 1036, 1092, 1249, 1578, 1639, 2644 |
| PspOMI | GGGCCC | 1 cut(s) | 2364 |
| PspPPI | RGGWCCY | 2 cut(s) | 1643, 2210 |
| PstI | CTGCAG | 1 cut(s) | 1590 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1585, 1793 |
| PsuI | RGATCY | 2 cut(s) | 563, 1558 |
| PvuII | CAGCTG | 2 cut(s) | 1088, 2371 |
| RsaI | GTAC | 7 cut(s) | 54, 431, 459, 930, 1255, 1970, 2398 |
| RsaNI | GTAC | 7 cut(s) | 53, 430, 458, 929, 1254, 1969, 2397 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2429, 2490 |
| SapI | GCTCTTC | 2 cut(s) | 1021, 1116 |
| SaqAI | TTAA | 9 cut(s) | 374, 990, 1469, 1629, 1683, 1803, 2090, 2352, 2421 |
| SchI | GAGTC | 2 cut(s) | 689, 908 |
| SduI | GDGCHC | 4 cut(s) | 591, 696, 2368, 2604 |
| SexAI | ACCWGGT | 2 cut(s) | 1036, 1249 |
| SfaNI | GCATC | 3 cut(s) | 883, 1606, 2400 |
| SfcI | CTRYAG | 2 cut(s) | 1271, 1586 |
| SfoI | GGCGCC | 1 cut(s) | 1159 |
| SinI | GGWCC | 6 cut(s) | 490, 1267, 1334, 1601, 1643, 2210 |
| SmaI | CCCGGG | 1 cut(s) | 1839 |
| SmiI | ATTTAAAT | 1 cut(s) | 375 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2429, 2490 |
| SmlI | CTYRAG | 1 cut(s) | 1682 |
| SmoI | CTYRAG | 1 cut(s) | 1682 |
| SpeI | ACTAGT | 2 cut(s) | 2426, 2453 |
| SphI | GCATGC | 1 cut(s) | 1430 |
| SspDI | GGCGCC | 1 cut(s) | 1157 |
| StyI | CCWWGG | 2 cut(s) | 2206, 2552 |
| SwaI | ATTTAAAT | 1 cut(s) | 375 |
| TaaI | ACNGT | 5 cut(s) | 8, 130, 871, 1951, 2593 |
| TaiI | ACGT | 7 cut(s) | 416, 431, 463, 1002, 1920, 2118, 2250 |
| TaqI | TCGA | 4 cut(s) | 275, 425, 1306, 1782 |
| TatI | WGTACW | 2 cut(s) | 52, 928 |
| TauI | GCSGC | 3 cut(s) | 162, 256, 852 |
| TfiI | GAWTC | 5 cut(s) | 67, 764, 779, 1303, 2496 |
| Tru1I | TTAA | 9 cut(s) | 374, 990, 1469, 1629, 1683, 1803, 2090, 2352, 2421 |
| Tru9I | TTAA | 9 cut(s) | 374, 990, 1469, 1629, 1683, 1803, 2090, 2352, 2421 |
| TscAI | CASTG | 7 cut(s) | 78, 696, 876, 918, 1737, 1954, 2275 |
| TseFI | GTSAC | 3 cut(s) | 124, 719, 1918 |
| Tsp45I | GTSAC | 3 cut(s) | 124, 719, 1918 |
| TspDTI | ATGAA | 8 cut(s) | 17, 301, 777, 792, 1220, 1428, 2474, 2480 |
| TspGWI | ACGGA | 3 cut(s) | 844, 1018, 2406 |
| TspMI | CCCGGG | 1 cut(s) | 1837 |
| TspRI | CASTG | 7 cut(s) | 78, 696, 876, 918, 1737, 1954, 2275 |
| Vha464I | CTTAAG | 1 cut(s) | 1682 |
| VpaK11BI | GGWCC | 6 cut(s) | 490, 1267, 1334, 1601, 1643, 2210 |
| VspI | ATTAAT | 1 cut(s) | 1629 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1166 |
| XapI | RAATTY | 2 cut(s) | 370, 1175 |
| XbaI | TCTAGA | 1 cut(s) | 1963 |
| XceI | RCATGY | 1 cut(s) | 1430 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 565, 2213 |
| XmaI | CCCGGG | 1 cut(s) | 1837 |
| XmaJI | CCTAGG | 1 cut(s) | 2552 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1179 |