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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 809, 841 |
| AciI | CCGC | 1 cut(s) | 739 |
| AclWI | GGATC | 2 cut(s) | 535, 855 |
| AcoI | YGGCCR | 1 cut(s) | 897 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AgsI | TTSAA | 3 cut(s) | 137, 185, 383 |
| AhlI | ACTAGT | 1 cut(s) | 83 |
| AjnI | CCWGG | 2 cut(s) | 174, 458 |
| AleI | CACNNNNGTG | 1 cut(s) | 281 |
| AluBI | AGCT | 4 cut(s) | 140, 308, 316, 907 |
| AluI | AGCT | 4 cut(s) | 140, 308, 316, 907 |
| Alw21I | GWGCWC | 1 cut(s) | 568 |
| Alw26I | GTCTC | 3 cut(s) | 547, 707, 827 |
| AlwI | GGATC | 2 cut(s) | 535, 855 |
| AoxI | GGCC | 1 cut(s) | 897 |
| ApeKI | GCWGC | 2 cut(s) | 260, 305 |
| AseI | ATTAAT | 1 cut(s) | 108 |
| AspS9I | GGNCC | 2 cut(s) | 456, 771 |
| AsuHPI | GGTGA | 3 cut(s) | 64, 198, 504 |
| AvaII | GGWCC | 2 cut(s) | 456, 771 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 433, 465 |
| BbsI | GAAGAC | 4 cut(s) | 114, 608, 688, 807 |
| Bbv12I | GWGCWC | 1 cut(s) | 568 |
| BbvI | GCAGC | 2 cut(s) | 247, 317 |
| BccI | CCATC | 4 cut(s) | 392, 518, 669, 876 |
| BceAI | ACGGC | 2 cut(s) | 732, 794 |
| BciT130I | CCWGG | 2 cut(s) | 176, 460 |
| BcoDI | GTCTC | 3 cut(s) | 547, 707, 827 |
| BcuI | ACTAGT | 1 cut(s) | 83 |
| BfaI | CTAG | 4 cut(s) | 69, 84, 435, 855 |
| BfmI | CTRYAG | 1 cut(s) | 261 |
| BisI | GCNGC | 2 cut(s) | 261, 306 |
| BlsI | GCNGC | 2 cut(s) | 262, 307 |
| Bme1390I | CCNGG | 2 cut(s) | 176, 460 |
| Bme18I | GGWCC | 2 cut(s) | 456, 771 |
| BmgT120I | GGNCC | 2 cut(s) | 456, 771 |
| BmiI | GGNNCC | 3 cut(s) | 772, 808, 894 |
| BmrFI | CCNGG | 2 cut(s) | 176, 460 |
| BmsI | GCATC | 1 cut(s) | 662 |
| BpiI | GAAGAC | 4 cut(s) | 114, 608, 688, 807 |
| BpmI | CTGGAG | 1 cut(s) | 754 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 3 cut(s) | 174, 346, 766 |
| BsaWI | WCCGGW | 1 cut(s) | 911 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 733, 763 |
| Bse118I | RCCGGY | 1 cut(s) | 895 |
| Bse1I | ACTGG | 1 cut(s) | 737 |
| BseBI | CCWGG | 2 cut(s) | 176, 460 |
| BseDI | CCNNGG | 3 cut(s) | 174, 346, 766 |
| BseGI | GGATG | 2 cut(s) | 529, 653 |
| BseMII | CTCAG | 2 cut(s) | 90, 192 |
| BseNI | ACTGG | 1 cut(s) | 737 |
| BseRI | GAGGAG | 2 cut(s) | 428, 497 |
| BseXI | GCAGC | 2 cut(s) | 247, 317 |
| BshFI | GGCC | 1 cut(s) | 899 |
| BsiHKAI | GWGCWC | 1 cut(s) | 568 |
| BsiSI | CCGG | 2 cut(s) | 896, 912 |
| BslFI | GGGAC | 1 cut(s) | 784 |
| BsmAI | GTCTC | 3 cut(s) | 547, 707, 827 |
| BsmBI | CGTCTC | 2 cut(s) | 547, 707 |
| BsmFI | GGGAC | 1 cut(s) | 784 |
| BsnI | GGCC | 1 cut(s) | 899 |
| Bsp1286I | GDGCHC | 1 cut(s) | 568 |
| Bsp143I | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| Bsp19I | CCATGG | 1 cut(s) | 346 |
| BspACI | CCGC | 1 cut(s) | 739 |
| BspANI | GGCC | 1 cut(s) | 899 |
| BspCNI | CTCAG | 2 cut(s) | 91, 193 |
| BspLI | GGNNCC | 3 cut(s) | 772, 808, 894 |
| BspMAI | CTGCAG | 1 cut(s) | 265 |
| BspPI | GGATC | 2 cut(s) | 535, 855 |
| BspQI | GCTCTTC | 1 cut(s) | 556 |
| BsrFI | RCCGGY | 1 cut(s) | 895 |
| BsrI | ACTGG | 1 cut(s) | 737 |
| BssAI | RCCGGY | 1 cut(s) | 895 |
| BssECI | CCNNGG | 3 cut(s) | 174, 346, 766 |
| BssMI | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 1 cut(s) | 346 |
| Bst2UI | CCWGG | 2 cut(s) | 176, 460 |
| Bst6I | CTCTTC | 2 cut(s) | 474, 556 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstC8I | GCNNGC | 2 cut(s) | 258, 897 |
| BstDEI | CTNAG | 4 cut(s) | 99, 201, 746, 903 |
| BstDSI | CCRYGG | 1 cut(s) | 346 |
| BstF5I | GGATG | 2 cut(s) | 529, 653 |
| BstKTI | GATC | 8 cut(s) | 504, 530, 551, 591, 631, 671, 863, 885 |
| BstMAI | GTCTC | 3 cut(s) | 547, 707, 827 |
| BstMBI | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| BstNI | CCWGG | 2 cut(s) | 176, 460 |
| BstSCI | CCNGG | 2 cut(s) | 174, 458 |
| BstSFI | CTRYAG | 1 cut(s) | 261 |
| BstV1I | GCAGC | 2 cut(s) | 247, 317 |
| BstV2I | GAAGAC | 4 cut(s) | 114, 608, 688, 807 |
| BstX2I | RGATCY | 1 cut(s) | 860 |
| BstYI | RGATCY | 1 cut(s) | 860 |
| BsuRI | GGCC | 1 cut(s) | 899 |
| BtgI | CCRYGG | 1 cut(s) | 346 |
| BtsCI | GGATG | 2 cut(s) | 529, 653 |
| BtsIMutI | CAGTG | 1 cut(s) | 791 |
| Cac8I | GCNNGC | 2 cut(s) | 258, 897 |
| Cfr10I | RCCGGY | 1 cut(s) | 895 |
| Cfr13I | GGNCC | 2 cut(s) | 456, 771 |
| CseI | GACGC | 3 cut(s) | 562, 602, 682 |
| CviAII | CATG | 4 cut(s) | 126, 347, 410, 453 |
| DdeI | CTNAG | 4 cut(s) | 99, 201, 746, 903 |
| DpnI | GATC | 8 cut(s) | 503, 529, 550, 590, 630, 670, 862, 884 |
| DpnII | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 809, 841 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 809, 841 |
| EaeI | YGGCCR | 1 cut(s) | 897 |
| Eam1104I | CTCTTC | 2 cut(s) | 474, 556 |
| EarI | CTCTTC | 2 cut(s) | 474, 556 |
| EciI | GGCGGA | 1 cut(s) | 754 |
| Eco130I | CCWWGG | 1 cut(s) | 346 |
| Eco47I | GGWCC | 2 cut(s) | 456, 771 |
| EcoO109I | RGGNCCY | 1 cut(s) | 771 |
| EcoRII | CCWGG | 2 cut(s) | 174, 458 |
| EcoT14I | CCWWGG | 1 cut(s) | 346 |
| ErhI | CCWWGG | 1 cut(s) | 346 |
| Esp3I | CGTCTC | 2 cut(s) | 547, 707 |
| FaeI | CATG | 4 cut(s) | 129, 350, 413, 456 |
| FaqI | GGGAC | 1 cut(s) | 784 |
| FatI | CATG | 4 cut(s) | 125, 346, 409, 452 |
| FauNDI | CATATG | 1 cut(s) | 293 |
| Fnu4HI | GCNGC | 2 cut(s) | 261, 306 |
| FokI | GGATG | 2 cut(s) | 536, 640 |
| Fsp4HI | GCNGC | 2 cut(s) | 261, 306 |
| FspBI | CTAG | 4 cut(s) | 69, 84, 435, 855 |
| GluI | GCNGC | 2 cut(s) | 261, 306 |
| GsuI | CTGGAG | 1 cut(s) | 754 |
| HaeIII | GGCC | 1 cut(s) | 899 |
| HapII | CCGG | 2 cut(s) | 896, 912 |
| HgaI | GACGC | 3 cut(s) | 562, 602, 682 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 4 cut(s) | 129, 350, 413, 456 |
| HincII | GTYRAC | 2 cut(s) | 223, 763 |
| HindII | GTYRAC | 2 cut(s) | 223, 763 |
| HindIII | AAGCTT | 1 cut(s) | 905 |
| HinfI | GANTC | 2 cut(s) | 203, 937 |
| HpaII | CCGG | 2 cut(s) | 896, 912 |
| HphI | GGTGA | 3 cut(s) | 64, 198, 504 |
| Hpy166II | GTNNAC | 5 cut(s) | 223, 534, 763, 814, 846 |
| Hpy188I | TCNGA | 1 cut(s) | 730 |
| Hpy188III | TCNNGA | 8 cut(s) | 69, 505, 552, 592, 632, 672, 712, 886 |
| Hpy8I | GTNNAC | 5 cut(s) | 223, 534, 763, 814, 846 |
| Hpy99I | CGWCG | 1 cut(s) | 10 |
| HpyAV | CCTTC | 2 cut(s) | 49, 715 |
| HpyCH4IV | ACGT | 1 cut(s) | 278 |
| HpyCH4V | TGCA | 2 cut(s) | 263, 332 |
| HpyF3I | CTNAG | 4 cut(s) | 99, 201, 746, 903 |
| HpySE526I | ACGT | 1 cut(s) | 278 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 4 cut(s) | 129, 350, 413, 456 |
| KroI | GCCGGC | 1 cut(s) | 895 |
| KroNI | GCCGGC | 1 cut(s) | 897 |
| Kzo9I | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| LguI | GCTCTTC | 1 cut(s) | 556 |
| LmnI | GCTCC | 3 cut(s) | 145, 806, 892 |
| Lsp1109I | GCAGC | 2 cut(s) | 247, 317 |
| LweI | GCATC | 1 cut(s) | 662 |
| MaeI | CTAG | 4 cut(s) | 69, 84, 435, 855 |
| MaeII | ACGT | 1 cut(s) | 278 |
| MaeIII | GTNAC | 7 cut(s) | 79, 204, 231, 274, 425, 440, 934 |
| MalI | GATC | 8 cut(s) | 503, 529, 550, 590, 630, 670, 862, 884 |
| MboI | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| MboII | GAAGA | 8 cut(s) | 114, 311, 365, 491, 573, 613, 693, 812 |
| MflI | RGATCY | 1 cut(s) | 860 |
| MhlI | GDGCHC | 1 cut(s) | 568 |
| MluCI | AATT | 3 cut(s) | 105, 213, 327 |
| MlyI | GAGTC | 2 cut(s) | 212, 931 |
| MroNI | GCCGGC | 1 cut(s) | 895 |
| MseI | TTAA | 1 cut(s) | 108 |
| MslI | CAYNNNNRTG | 1 cut(s) | 281 |
| MspI | CCGG | 2 cut(s) | 896, 912 |
| MspR9I | CCNGG | 2 cut(s) | 176, 460 |
| MvaI | CCWGG | 2 cut(s) | 176, 460 |
| NaeI | GCCGGC | 1 cut(s) | 897 |
| NcoI | CCATGG | 1 cut(s) | 346 |
| NdeI | CATATG | 1 cut(s) | 293 |
| NdeII | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| NgoMIV | GCCGGC | 1 cut(s) | 895 |
| NlaIII | CATG | 4 cut(s) | 129, 350, 413, 456 |
| NlaIV | GGNNCC | 3 cut(s) | 772, 808, 894 |
| NmuCI | GTSAC | 4 cut(s) | 204, 274, 425, 934 |
| OliI | CACNNNNGTG | 1 cut(s) | 281 |
| PciSI | GCTCTTC | 1 cut(s) | 556 |
| PdiI | GCCGGC | 1 cut(s) | 897 |
| PfoI | TCCNGGA | 1 cut(s) | 458 |
| PkrI | GCNGC | 2 cut(s) | 262, 307 |
| PleI | GAGTC | 2 cut(s) | 211, 931 |
| PpsI | GAGTC | 2 cut(s) | 211, 931 |
| PpuMI | RGGWCCY | 1 cut(s) | 771 |
| PshBI | ATTAAT | 1 cut(s) | 108 |
| Psp5II | RGGWCCY | 1 cut(s) | 771 |
| Psp6I | CCWGG | 2 cut(s) | 174, 458 |
| PspGI | CCWGG | 2 cut(s) | 174, 458 |
| PspN4I | GGNNCC | 3 cut(s) | 772, 808, 894 |
| PspPI | GGNCC | 2 cut(s) | 456, 771 |
| PspPPI | RGGWCCY | 1 cut(s) | 771 |
| PstI | CTGCAG | 1 cut(s) | 265 |
| PsuI | RGATCY | 1 cut(s) | 860 |
| RseI | CAYNNNNRTG | 1 cut(s) | 281 |
| SapI | GCTCTTC | 1 cut(s) | 556 |
| SaqAI | TTAA | 1 cut(s) | 108 |
| SatI | GCNGC | 2 cut(s) | 261, 306 |
| Sau3AI | GATC | 8 cut(s) | 501, 527, 548, 588, 628, 668, 860, 882 |
| Sau96I | GGNCC | 2 cut(s) | 456, 771 |
| SchI | GAGTC | 2 cut(s) | 212, 931 |
| ScrFI | CCNGG | 2 cut(s) | 176, 460 |
| SduI | GDGCHC | 1 cut(s) | 568 |
| SfaNI | GCATC | 1 cut(s) | 662 |
| SfcI | CTRYAG | 1 cut(s) | 261 |
| SinI | GGWCC | 2 cut(s) | 456, 771 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 281 |
| SpeI | ACTAGT | 1 cut(s) | 83 |
| Sse9I | AATT | 3 cut(s) | 105, 213, 327 |
| SsiI | CCGC | 1 cut(s) | 739 |
| SspMI | CTAG | 4 cut(s) | 69, 84, 435, 855 |
| StyD4I | CCNGG | 2 cut(s) | 174, 458 |
| StyI | CCWWGG | 1 cut(s) | 346 |
| TaiI | ACGT | 1 cut(s) | 281 |
| TaqI | TCGA | 4 cut(s) | 165, 551, 671, 711 |
| TaqII | GACCGA | 1 cut(s) | 780 |
| TasI | AATT | 3 cut(s) | 105, 213, 327 |
| Tru1I | TTAA | 1 cut(s) | 108 |
| Tru9I | TTAA | 1 cut(s) | 108 |
| TscAI | CASTG | 1 cut(s) | 798 |
| TseFI | GTSAC | 4 cut(s) | 204, 274, 425, 934 |
| TseI | GCWGC | 2 cut(s) | 260, 305 |
| Tsp45I | GTSAC | 4 cut(s) | 204, 274, 425, 934 |
| TspDTI | ATGAA | 4 cut(s) | 54, 114, 372, 441 |
| TspGWI | ACGGA | 5 cut(s) | 571, 611, 819, 853, 905 |
| TspRI | CASTG | 1 cut(s) | 798 |
| VpaK11BI | GGWCC | 2 cut(s) | 456, 771 |
| VspI | ATTAAT | 1 cut(s) | 108 |
| XbaI | TCTAGA | 1 cut(s) | 68 |
| XspI | CTAG | 4 cut(s) | 69, 84, 435, 855 |