Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 74, 170 |
| AclWI | GGATC | 1 cut(s) | 115 |
| AcoI | YGGCCR | 1 cut(s) | 286 |
| AfaI | GTAC | 1 cut(s) | 449 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 274 |
| AflIII | ACRYGT | 1 cut(s) | 463 |
| AgeI | ACCGGT | 1 cut(s) | 85 |
| AjnI | CCWGG | 1 cut(s) | 165 |
| AluBI | AGCT | 3 cut(s) | 299, 521, 566 |
| AluI | AGCT | 3 cut(s) | 299, 521, 566 |
| Alw26I | GTCTC | 2 cut(s) | 99, 426 |
| AlwI | GGATC | 1 cut(s) | 115 |
| AoxI | GGCC | 2 cut(s) | 54, 286 |
| AsiGI | ACCGGT | 1 cut(s) | 85 |
| AspS9I | GGNCC | 1 cut(s) | 55 |
| AsuHPI | GGTGA | 2 cut(s) | 36, 407 |
| BalI | TGGCCA | 1 cut(s) | 288 |
| BbsI | GAAGAC | 1 cut(s) | 200 |
| BccI | CCATC | 3 cut(s) | 118, 122, 403 |
| BciT130I | CCWGG | 1 cut(s) | 167 |
| BciVI | GTATCC | 1 cut(s) | 155 |
| BcoDI | GTCTC | 2 cut(s) | 99, 426 |
| BfaI | CTAG | 3 cut(s) | 176, 222, 501 |
| BfmI | CTRYAG | 1 cut(s) | 243 |
| BfuI | GTATCC | 1 cut(s) | 155 |
| BglII | AGATCT | 2 cut(s) | 247, 503 |
| Bme1390I | CCNGG | 1 cut(s) | 167 |
| BmgT120I | GGNCC | 1 cut(s) | 55 |
| BmrFI | CCNGG | 1 cut(s) | 167 |
| BmsI | GCATC | 2 cut(s) | 349, 371 |
| BpiI | GAAGAC | 1 cut(s) | 200 |
| BsaI | GGTCTC | 1 cut(s) | 426 |
| BsaJI | CCNNGG | 1 cut(s) | 69 |
| BsaWI | WCCGGW | 1 cut(s) | 85 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 274 |
| Bse118I | RCCGGY | 1 cut(s) | 85 |
| Bse1I | ACTGG | 2 cut(s) | 204, 289 |
| BseBI | CCWGG | 1 cut(s) | 167 |
| BseDI | CCNNGG | 1 cut(s) | 69 |
| BseGI | GGATG | 1 cut(s) | 364 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 274 |
| BseMII | CTCAG | 3 cut(s) | 144, 324, 521 |
| BseNI | ACTGG | 2 cut(s) | 204, 289 |
| BshFI | GGCC | 2 cut(s) | 56, 288 |
| BshTI | ACCGGT | 1 cut(s) | 85 |
| BsiSI | CCGG | 1 cut(s) | 86 |
| BslFI | GGGAC | 1 cut(s) | 186 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 274 |
| BsmAI | GTCTC | 2 cut(s) | 99, 426 |
| BsmFI | GGGAC | 1 cut(s) | 186 |
| BsnI | GGCC | 2 cut(s) | 56, 288 |
| Bso31I | GGTCTC | 1 cut(s) | 426 |
| Bsp143I | GATC | 3 cut(s) | 120, 247, 503 |
| BspACI | CCGC | 2 cut(s) | 74, 170 |
| BspANI | GGCC | 2 cut(s) | 56, 288 |
| BspCNI | CTCAG | 3 cut(s) | 145, 325, 520 |
| BspPI | GGATC | 1 cut(s) | 115 |
| BspTNI | GGTCTC | 1 cut(s) | 426 |
| BsrFI | RCCGGY | 1 cut(s) | 85 |
| BsrI | ACTGG | 2 cut(s) | 204, 289 |
| BssAI | RCCGGY | 1 cut(s) | 85 |
| BssECI | CCNNGG | 1 cut(s) | 69 |
| BssMI | GATC | 3 cut(s) | 120, 247, 503 |
| Bst2UI | CCWGG | 1 cut(s) | 167 |
| Bst4CI | ACNGT | 2 cut(s) | 490, 532 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 297 |
| BstDEI | CTNAG | 3 cut(s) | 153, 333, 507 |
| BstF5I | GGATG | 1 cut(s) | 364 |
| BstKTI | GATC | 3 cut(s) | 123, 250, 506 |
| BstMAI | GTCTC | 2 cut(s) | 99, 426 |
| BstMBI | GATC | 3 cut(s) | 120, 247, 503 |
| BstNI | CCWGG | 1 cut(s) | 167 |
| BstNSI | RCATGY | 1 cut(s) | 403 |
| BstSCI | CCNGG | 1 cut(s) | 165 |
| BstSFI | CTRYAG | 1 cut(s) | 243 |
| BstV2I | GAAGAC | 1 cut(s) | 200 |
| BstX2I | RGATCY | 2 cut(s) | 247, 503 |
| BstYI | RGATCY | 2 cut(s) | 247, 503 |
| BsuI | GTATCC | 1 cut(s) | 155 |
| BsuRI | GGCC | 2 cut(s) | 56, 288 |
| BtgZI | GCGATG | 1 cut(s) | 96 |
| BtsCI | GGATG | 1 cut(s) | 364 |
| BtsIMutI | CAGTG | 2 cut(s) | 296, 330 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 297 |
| Cfr10I | RCCGGY | 1 cut(s) | 85 |
| Cfr13I | GGNCC | 1 cut(s) | 55 |
| CseI | GACGC | 1 cut(s) | 503 |
| Csp6I | GTAC | 1 cut(s) | 448 |
| CspAI | ACCGGT | 1 cut(s) | 85 |
| CviAII | CATG | 4 cut(s) | 400, 404, 476, 496 |
| CviQI | GTAC | 1 cut(s) | 448 |
| DdeI | CTNAG | 3 cut(s) | 153, 333, 507 |
| DpnI | GATC | 3 cut(s) | 122, 249, 505 |
| DpnII | GATC | 3 cut(s) | 120, 247, 503 |
| EaeI | YGGCCR | 1 cut(s) | 286 |
| Eco31I | GGTCTC | 1 cut(s) | 426 |
| EcoRII | CCWGG | 1 cut(s) | 165 |
| EcoT22I | ATGCAT | 2 cut(s) | 364, 405 |
| FaeI | CATG | 4 cut(s) | 403, 407, 479, 499 |
| FaqI | GGGAC | 1 cut(s) | 186 |
| FatI | CATG | 4 cut(s) | 399, 403, 475, 495 |
| FauI | CCCGC | 1 cut(s) | 163 |
| FauNDI | CATATG | 2 cut(s) | 192, 213 |
| FokI | GGATG | 1 cut(s) | 371 |
| FspBI | CTAG | 3 cut(s) | 176, 222, 501 |
| HaeIII | GGCC | 2 cut(s) | 56, 288 |
| HapII | CCGG | 1 cut(s) | 86 |
| HgaI | GACGC | 1 cut(s) | 503 |
| Hin1II | CATG | 4 cut(s) | 403, 407, 479, 499 |
| HincII | GTYRAC | 1 cut(s) | 319 |
| HindII | GTYRAC | 1 cut(s) | 319 |
| HinfI | GANTC | 3 cut(s) | 41, 347, 455 |
| HpaI | GTTAAC | 1 cut(s) | 319 |
| HpaII | CCGG | 1 cut(s) | 86 |
| HphI | GGTGA | 2 cut(s) | 36, 407 |
| Hpy166II | GTNNAC | 2 cut(s) | 319, 486 |
| Hpy188I | TCNGA | 6 cut(s) | 33, 154, 352, 367, 460, 510 |
| Hpy188III | TCNNGA | 2 cut(s) | 501, 539 |
| Hpy8I | GTNNAC | 2 cut(s) | 319, 486 |
| HpyAV | CCTTC | 3 cut(s) | 152, 307, 560 |
| HpyCH4III | ACNGT | 2 cut(s) | 490, 532 |
| HpyCH4IV | ACGT | 2 cut(s) | 36, 463 |
| HpyCH4V | TGCA | 3 cut(s) | 82, 362, 403 |
| HpyF3I | CTNAG | 3 cut(s) | 153, 333, 507 |
| HpySE526I | ACGT | 2 cut(s) | 36, 463 |
| Hsp92II | CATG | 4 cut(s) | 403, 407, 479, 499 |
| KspAI | GTTAAC | 1 cut(s) | 319 |
| Kzo9I | GATC | 3 cut(s) | 120, 247, 503 |
| LpnPI | CCDG | 8 cut(s) | 38, 99, 152, 179, 185, 255, 302, 323 |
| LweI | GCATC | 2 cut(s) | 349, 371 |
| MaeI | CTAG | 3 cut(s) | 176, 222, 501 |
| MaeII | ACGT | 2 cut(s) | 36, 463 |
| MaeIII | GTNAC | 4 cut(s) | 42, 105, 195, 395 |
| MalI | GATC | 3 cut(s) | 122, 249, 505 |
| MboI | GATC | 3 cut(s) | 120, 247, 503 |
| MboII | GAAGA | 2 cut(s) | 200, 242 |
| MflI | RGATCY | 2 cut(s) | 247, 503 |
| MlsI | TGGCCA | 1 cut(s) | 288 |
| MluNI | TGGCCA | 1 cut(s) | 288 |
| MlyI | GAGTC | 1 cut(s) | 50 |
| MnlI | CCTC | 7 cut(s) | 79, 148, 160, 272, 367, 371, 535 |
| Mox20I | TGGCCA | 1 cut(s) | 288 |
| Mph1103I | ATGCAT | 2 cut(s) | 364, 405 |
| MscI | TGGCCA | 1 cut(s) | 288 |
| MseI | TTAA | 2 cut(s) | 102, 318 |
| MslI | CAYNNNNRTG | 1 cut(s) | 63 |
| Msp20I | TGGCCA | 1 cut(s) | 288 |
| MspI | CCGG | 1 cut(s) | 86 |
| MspR9I | CCNGG | 1 cut(s) | 167 |
| MvaI | CCWGG | 1 cut(s) | 167 |
| NdeI | CATATG | 2 cut(s) | 192, 213 |
| NdeII | GATC | 3 cut(s) | 120, 247, 503 |
| NlaIII | CATG | 4 cut(s) | 403, 407, 479, 499 |
| NmeAIII | GCCGAG | 1 cut(s) | 50 |
| NmuCI | GTSAC | 2 cut(s) | 42, 395 |
| NsiI | ATGCAT | 2 cut(s) | 364, 405 |
| NspI | RCATGY | 1 cut(s) | 403 |
| PfeI | GAWTC | 2 cut(s) | 347, 455 |
| PinAI | ACCGGT | 1 cut(s) | 85 |
| PleI | GAGTC | 1 cut(s) | 49 |
| PpsI | GAGTC | 1 cut(s) | 49 |
| Psp6I | CCWGG | 1 cut(s) | 165 |
| PspGI | CCWGG | 1 cut(s) | 165 |
| PspPI | GGNCC | 1 cut(s) | 55 |
| PsuI | RGATCY | 2 cut(s) | 247, 503 |
| RsaI | GTAC | 1 cut(s) | 449 |
| RsaNI | GTAC | 1 cut(s) | 448 |
| RseI | CAYNNNNRTG | 1 cut(s) | 63 |
| SaqAI | TTAA | 2 cut(s) | 102, 318 |
| Sau3AI | GATC | 3 cut(s) | 120, 247, 503 |
| Sau96I | GGNCC | 1 cut(s) | 55 |
| SchI | GAGTC | 1 cut(s) | 50 |
| ScrFI | CCNGG | 1 cut(s) | 167 |
| SfaNI | GCATC | 2 cut(s) | 349, 371 |
| SfcI | CTRYAG | 1 cut(s) | 243 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 63 |
| SsiI | CCGC | 2 cut(s) | 74, 170 |
| SspMI | CTAG | 3 cut(s) | 176, 222, 501 |
| StyD4I | CCNGG | 1 cut(s) | 165 |
| TaaI | ACNGT | 2 cut(s) | 490, 532 |
| TaiI | ACGT | 2 cut(s) | 39, 466 |
| TaqI | TCGA | 1 cut(s) | 538 |
| TfiI | GAWTC | 2 cut(s) | 347, 455 |
| Tru1I | TTAA | 2 cut(s) | 102, 318 |
| Tru9I | TTAA | 2 cut(s) | 102, 318 |
| TscAI | CASTG | 2 cut(s) | 296, 337 |
| TseFI | GTSAC | 2 cut(s) | 42, 395 |
| Tsp45I | GTSAC | 2 cut(s) | 42, 395 |
| TspDTI | ATGAA | 4 cut(s) | 200, 468, 486, 542 |
| TspRI | CASTG | 2 cut(s) | 296, 337 |
| XbaI | TCTAGA | 1 cut(s) | 500 |
| XceI | RCATGY | 1 cut(s) | 403 |
| XspI | CTAG | 3 cut(s) | 176, 222, 501 |
| Zsp2I | ATGCAT | 2 cut(s) | 364, 405 |