Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 67, 456 |
| AccI | GTMKAC | 1 cut(s) | 589 |
| AciI | CCGC | 2 cut(s) | 378, 694 |
| AclWI | GGATC | 1 cut(s) | 615 |
| AcsI | RAATTY | 2 cut(s) | 293, 358 |
| AcuI | CTGAAG | 1 cut(s) | 171 |
| AfaI | GTAC | 3 cut(s) | 6, 138, 748 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 67, 383, 456, 559, 649, 779, 795, 829 |
| AgsI | TTSAA | 4 cut(s) | 312, 346, 444, 667 |
| AjnI | CCWGG | 4 cut(s) | 102, 118, 198, 644 |
| AluBI | AGCT | 2 cut(s) | 89, 683 |
| AluI | AGCT | 2 cut(s) | 89, 683 |
| AlwI | GGATC | 1 cut(s) | 615 |
| AoxI | GGCC | 2 cut(s) | 201, 640 |
| ApeKI | GCWGC | 1 cut(s) | 680 |
| ApoI | RAATTY | 2 cut(s) | 293, 358 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 81 |
| AspS9I | GGNCC | 2 cut(s) | 641, 798 |
| AvaII | GGWCC | 1 cut(s) | 798 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 66, 98 |
| BbvI | GCAGC | 1 cut(s) | 692 |
| BccI | CCATC | 2 cut(s) | 202, 530 |
| BciT130I | CCWGG | 4 cut(s) | 104, 120, 200, 646 |
| BciVI | GTATCC | 1 cut(s) | 560 |
| BclI | TGATCA | 2 cut(s) | 37, 52 |
| BfaI | CTAG | 4 cut(s) | 270, 627, 677, 777 |
| BfmI | CTRYAG | 1 cut(s) | 72 |
| BfuI | GTATCC | 1 cut(s) | 560 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 209 |
| BisI | GCNGC | 1 cut(s) | 681 |
| BlsI | GCNGC | 1 cut(s) | 682 |
| Bme1390I | CCNGG | 4 cut(s) | 104, 120, 200, 646 |
| Bme18I | GGWCC | 1 cut(s) | 798 |
| BmgT120I | GGNCC | 2 cut(s) | 641, 798 |
| BmiI | GGNNCC | 1 cut(s) | 643 |
| BmrFI | CCNGG | 4 cut(s) | 104, 120, 200, 646 |
| BmsI | GCATC | 1 cut(s) | 181 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 218, 250 |
| BsaAI | YACGTR | 2 cut(s) | 43, 371 |
| BsaBI | GATNNNNATC | 1 cut(s) | 612 |
| BsaJI | CCNNGG | 4 cut(s) | 103, 198, 531, 644 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 67, 383, 456, 559, 649, 779, 795, 829 |
| Bse1I | ACTGG | 3 cut(s) | 27, 179, 485 |
| Bse8I | GATNNNNATC | 1 cut(s) | 612 |
| BseBI | CCWGG | 4 cut(s) | 104, 120, 200, 646 |
| BseDI | CCNNGG | 4 cut(s) | 103, 198, 531, 644 |
| BseGI | GGATG | 3 cut(s) | 469, 541, 768 |
| BseJI | GATNNNNATC | 1 cut(s) | 612 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 67, 383, 456, 559, 649, 779, 795, 829 |
| BseNI | ACTGG | 3 cut(s) | 27, 179, 485 |
| BseRI | GAGGAG | 1 cut(s) | 242 |
| BseXI | GCAGC | 1 cut(s) | 692 |
| BshFI | GGCC | 2 cut(s) | 203, 642 |
| BslFI | GGGAC | 2 cut(s) | 181, 420 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 67, 383, 456, 559, 649, 779, 795, 829 |
| BsmFI | GGGAC | 2 cut(s) | 181, 420 |
| BsnI | GGCC | 2 cut(s) | 203, 642 |
| Bsp1407I | TGTACA | 1 cut(s) | 136 |
| Bsp143I | GATC | 4 cut(s) | 37, 52, 607, 826 |
| BspACI | CCGC | 2 cut(s) | 378, 694 |
| BspANI | GGCC | 2 cut(s) | 203, 642 |
| BspHI | TCATGA | 1 cut(s) | 685 |
| BspLI | GGNNCC | 1 cut(s) | 643 |
| BspPI | GGATC | 1 cut(s) | 615 |
| BsrGI | TGTACA | 1 cut(s) | 136 |
| BsrI | ACTGG | 3 cut(s) | 27, 179, 485 |
| BssECI | CCNNGG | 4 cut(s) | 103, 198, 531, 644 |
| BssMI | GATC | 4 cut(s) | 37, 52, 607, 826 |
| BssT1I | CCWWGG | 1 cut(s) | 531 |
| Bst2UI | CCWGG | 4 cut(s) | 104, 120, 200, 646 |
| Bst4CI | ACNGT | 4 cut(s) | 136, 330, 411, 562 |
| Bst6I | CTCTTC | 1 cut(s) | 175 |
| BstAUI | TGTACA | 1 cut(s) | 136 |
| BstBAI | YACGTR | 2 cut(s) | 43, 371 |
| BstDEI | CTNAG | 1 cut(s) | 570 |
| BstF5I | GGATG | 3 cut(s) | 469, 541, 768 |
| BstKTI | GATC | 4 cut(s) | 40, 55, 610, 829 |
| BstMBI | GATC | 4 cut(s) | 37, 52, 607, 826 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 191, 200, 209 |
| BstNI | CCWGG | 4 cut(s) | 104, 120, 200, 646 |
| BstSCI | CCNGG | 4 cut(s) | 102, 118, 198, 644 |
| BstSFI | CTRYAG | 1 cut(s) | 72 |
| BstV1I | GCAGC | 1 cut(s) | 692 |
| BstX2I | RGATCY | 1 cut(s) | 607 |
| BstYI | RGATCY | 1 cut(s) | 607 |
| BsuI | GTATCC | 1 cut(s) | 560 |
| BsuRI | GGCC | 2 cut(s) | 203, 642 |
| BtsCI | GGATG | 3 cut(s) | 469, 541, 768 |
| BtsIMutI | CAGTG | 1 cut(s) | 492 |
| CciI | TCATGA | 1 cut(s) | 685 |
| Cfr13I | GGNCC | 2 cut(s) | 641, 798 |
| CseI | GACGC | 1 cut(s) | 183 |
| Csp6I | GTAC | 3 cut(s) | 5, 137, 747 |
| CviAII | CATG | 5 cut(s) | 156, 205, 546, 638, 686 |
| CviQI | GTAC | 3 cut(s) | 5, 137, 747 |
| DdeI | CTNAG | 1 cut(s) | 570 |
| DpnI | GATC | 4 cut(s) | 39, 54, 609, 828 |
| DpnII | GATC | 4 cut(s) | 37, 52, 607, 826 |
| Eam1104I | CTCTTC | 1 cut(s) | 175 |
| EarI | CTCTTC | 1 cut(s) | 175 |
| Eco130I | CCWWGG | 1 cut(s) | 531 |
| Eco32I | GATATC | 1 cut(s) | 151 |
| Eco47I | GGWCC | 1 cut(s) | 798 |
| Eco57I | CTGAAG | 1 cut(s) | 171 |
| EcoRII | CCWGG | 4 cut(s) | 102, 118, 198, 644 |
| EcoRV | GATATC | 1 cut(s) | 151 |
| EcoT14I | CCWWGG | 1 cut(s) | 531 |
| ErhI | CCWWGG | 1 cut(s) | 531 |
| FaeI | CATG | 5 cut(s) | 159, 208, 549, 641, 689 |
| FaqI | GGGAC | 2 cut(s) | 181, 420 |
| FatI | CATG | 5 cut(s) | 155, 204, 545, 637, 685 |
| FauI | CCCGC | 2 cut(s) | 385, 687 |
| FbaI | TGATCA | 2 cut(s) | 37, 52 |
| FblI | GTMKAC | 1 cut(s) | 589 |
| Fnu4HI | GCNGC | 1 cut(s) | 681 |
| FokI | GGATG | 3 cut(s) | 456, 548, 755 |
| Fsp4HI | GCNGC | 1 cut(s) | 681 |
| FspBI | CTAG | 4 cut(s) | 270, 627, 677, 777 |
| GluI | GCNGC | 1 cut(s) | 681 |
| HaeIII | GGCC | 2 cut(s) | 203, 642 |
| HgaI | GACGC | 1 cut(s) | 183 |
| Hin1II | CATG | 5 cut(s) | 159, 208, 549, 641, 689 |
| HinfI | GANTC | 1 cut(s) | 233 |
| Hpy166II | GTNNAC | 4 cut(s) | 161, 374, 590, 747 |
| Hpy188I | TCNGA | 3 cut(s) | 129, 190, 659 |
| Hpy188III | TCNNGA | 2 cut(s) | 473, 686 |
| Hpy8I | GTNNAC | 4 cut(s) | 161, 374, 590, 747 |
| HpyAV | CCTTC | 1 cut(s) | 281 |
| HpyCH4III | ACNGT | 4 cut(s) | 136, 330, 411, 562 |
| HpyCH4IV | ACGT | 3 cut(s) | 42, 370, 592 |
| HpyCH4V | TGCA | 1 cut(s) | 756 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 191, 200, 209 |
| HpyF3I | CTNAG | 1 cut(s) | 570 |
| HpySE526I | ACGT | 3 cut(s) | 42, 370, 592 |
| Hsp92II | CATG | 5 cut(s) | 159, 208, 549, 641, 689 |
| Ksp22I | TGATCA | 2 cut(s) | 37, 52 |
| Kzo9I | GATC | 4 cut(s) | 37, 52, 607, 826 |
| LmnI | GCTCC | 1 cut(s) | 533 |
| Lsp1109I | GCAGC | 1 cut(s) | 692 |
| LweI | GCATC | 1 cut(s) | 181 |
| MaeI | CTAG | 4 cut(s) | 270, 627, 677, 777 |
| MaeII | ACGT | 3 cut(s) | 42, 370, 592 |
| MalI | GATC | 4 cut(s) | 39, 54, 609, 828 |
| MboI | GATC | 4 cut(s) | 37, 52, 607, 826 |
| MboII | GAAGA | 5 cut(s) | 87, 162, 296, 489, 819 |
| MfeI | CAATTG | 1 cut(s) | 751 |
| MflI | RGATCY | 1 cut(s) | 607 |
| MluCI | AATT | 6 cut(s) | 293, 358, 618, 667, 712, 751 |
| MlyI | GAGTC | 1 cut(s) | 227 |
| MmeI | TCCRAC | 1 cut(s) | 760 |
| MroXI | GAANNNNTTC | 1 cut(s) | 81 |
| MseI | TTAA | 3 cut(s) | 332, 480, 612 |
| MslI | CAYNNNNRTG | 2 cut(s) | 45, 474 |
| MspA1I | CMGCKG | 1 cut(s) | 380 |
| MspR9I | CCNGG | 4 cut(s) | 104, 120, 200, 646 |
| MunI | CAATTG | 1 cut(s) | 751 |
| MvaI | CCWGG | 4 cut(s) | 104, 120, 200, 646 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 191, 200, 209 |
| NdeII | GATC | 4 cut(s) | 37, 52, 607, 826 |
| NlaIII | CATG | 5 cut(s) | 159, 208, 549, 641, 689 |
| NlaIV | GGNNCC | 1 cut(s) | 643 |
| PagI | TCATGA | 1 cut(s) | 685 |
| PdmI | GAANNNNTTC | 1 cut(s) | 81 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 67, 456 |
| PkrI | GCNGC | 1 cut(s) | 682 |
| PleI | GAGTC | 1 cut(s) | 227 |
| PpsI | GAGTC | 1 cut(s) | 227 |
| Ppu21I | YACGTR | 2 cut(s) | 43, 371 |
| Psp6I | CCWGG | 4 cut(s) | 102, 118, 198, 644 |
| PspGI | CCWGG | 4 cut(s) | 102, 118, 198, 644 |
| PspN4I | GGNNCC | 1 cut(s) | 643 |
| PspPI | GGNCC | 2 cut(s) | 641, 798 |
| PsuI | RGATCY | 1 cut(s) | 607 |
| RsaI | GTAC | 3 cut(s) | 6, 138, 748 |
| RsaNI | GTAC | 3 cut(s) | 5, 137, 747 |
| RseI | CAYNNNNRTG | 2 cut(s) | 45, 474 |
| SaqAI | TTAA | 3 cut(s) | 332, 480, 612 |
| SatI | GCNGC | 1 cut(s) | 681 |
| Sau3AI | GATC | 4 cut(s) | 37, 52, 607, 826 |
| Sau96I | GGNCC | 2 cut(s) | 641, 798 |
| SchI | GAGTC | 1 cut(s) | 227 |
| ScrFI | CCNGG | 4 cut(s) | 104, 120, 200, 646 |
| SetI | ASST | 9 cut(s) | 45, 91, 121, 271, 373, 595, 685, 703, 784 |
| SfaNI | GCATC | 1 cut(s) | 181 |
| SfcI | CTRYAG | 1 cut(s) | 72 |
| SinI | GGWCC | 1 cut(s) | 798 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 45, 474 |
| Sse9I | AATT | 6 cut(s) | 293, 358, 618, 667, 712, 751 |
| SsiI | CCGC | 2 cut(s) | 378, 694 |
| SspMI | CTAG | 4 cut(s) | 270, 627, 677, 777 |
| StyD4I | CCNGG | 4 cut(s) | 102, 118, 198, 644 |
| StyI | CCWWGG | 1 cut(s) | 531 |
| TaaI | ACNGT | 4 cut(s) | 136, 330, 411, 562 |
| TaiI | ACGT | 3 cut(s) | 45, 373, 595 |
| TaqII | GACCGA | 1 cut(s) | 815 |
| TasI | AATT | 6 cut(s) | 293, 358, 618, 667, 712, 751 |
| TatI | WGTACW | 1 cut(s) | 136 |
| Tru1I | TTAA | 3 cut(s) | 332, 480, 612 |
| Tru9I | TTAA | 3 cut(s) | 332, 480, 612 |
| TscAI | CASTG | 1 cut(s) | 492 |
| TseI | GCWGC | 1 cut(s) | 680 |
| TspDTI | ATGAA | 2 cut(s) | 444, 626 |
| TspRI | CASTG | 1 cut(s) | 492 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 67, 456 |
| VpaK11BI | GGWCC | 1 cut(s) | 798 |
| XapI | RAATTY | 2 cut(s) | 293, 358 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 724 |
| XmiI | GTMKAC | 1 cut(s) | 589 |
| XmnI | GAANNNNTTC | 1 cut(s) | 81 |
| XspI | CTAG | 4 cut(s) | 270, 627, 677, 777 |