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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 461 |
| Acc36I | ACCTGC | 1 cut(s) | 461 |
| AciI | CCGC | 1 cut(s) | 317 |
| AclWI | GGATC | 1 cut(s) | 168 |
| AcuI | CTGAAG | 1 cut(s) | 38 |
| AfaI | GTAC | 1 cut(s) | 62 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 200 |
| AgsI | TTSAA | 1 cut(s) | 383 |
| AhlI | ACTAGT | 1 cut(s) | 179 |
| AluBI | AGCT | 4 cut(s) | 16, 414, 478, 498 |
| AluI | AGCT | 4 cut(s) | 16, 414, 478, 498 |
| Alw21I | GWGCWC | 1 cut(s) | 416 |
| Alw26I | GTCTC | 1 cut(s) | 329 |
| AlwI | GGATC | 1 cut(s) | 168 |
| Ama87I | CYCGRG | 1 cut(s) | 409 |
| ApeKI | GCWGC | 2 cut(s) | 170, 467 |
| AspA2I | CCTAGG | 1 cut(s) | 248 |
| AspS9I | GGNCC | 2 cut(s) | 208, 253 |
| AsuHPI | GGTGA | 1 cut(s) | 485 |
| AvaI | CYCGRG | 1 cut(s) | 409 |
| AvaII | GGWCC | 2 cut(s) | 208, 253 |
| AvrII | CCTAGG | 1 cut(s) | 248 |
| BanII | GRGCYC | 1 cut(s) | 416 |
| Bbv12I | GWGCWC | 1 cut(s) | 416 |
| BbvI | GCAGC | 2 cut(s) | 157, 479 |
| BceAI | ACGGC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 1 cut(s) | 329 |
| BcuI | ACTAGT | 1 cut(s) | 179 |
| BfaI | CTAG | 5 cut(s) | 180, 249, 458, 495, 499 |
| BfuAI | ACCTGC | 1 cut(s) | 461 |
| BisI | GCNGC | 3 cut(s) | 171, 318, 468 |
| BlnI | CCTAGG | 1 cut(s) | 248 |
| BlsI | GCNGC | 3 cut(s) | 172, 319, 469 |
| Bme18I | GGWCC | 2 cut(s) | 208, 253 |
| BmeT110I | CYCGRG | 1 cut(s) | 409 |
| BmgT120I | GGNCC | 2 cut(s) | 208, 253 |
| BmiI | GGNNCC | 1 cut(s) | 254 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 392, 424 |
| BpuEI | CTTGAG | 3 cut(s) | 38, 403, 461 |
| BsaJI | CCNNGG | 1 cut(s) | 248 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 398, 428 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 200 |
| BseDI | CCNNGG | 1 cut(s) | 248 |
| BseGI | GGATG | 2 cut(s) | 331, 409 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 200 |
| BseMII | CTCAG | 1 cut(s) | 420 |
| BseRI | GAGGAG | 2 cut(s) | 406, 458 |
| BseXI | GCAGC | 2 cut(s) | 157, 479 |
| BseYI | CCCAGC | 1 cut(s) | 345 |
| BsiHKAI | GWGCWC | 1 cut(s) | 416 |
| BsiHKCI | CYCGRG | 1 cut(s) | 409 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 200 |
| BsmAI | GTCTC | 1 cut(s) | 329 |
| BsoBI | CYCGRG | 1 cut(s) | 409 |
| Bsp1286I | GDGCHC | 1 cut(s) | 416 |
| Bsp143I | GATC | 1 cut(s) | 160 |
| BspACI | CCGC | 1 cut(s) | 317 |
| BspCNI | CTCAG | 1 cut(s) | 421 |
| BspLI | GGNNCC | 1 cut(s) | 254 |
| BspMI | ACCTGC | 1 cut(s) | 461 |
| BspPI | GGATC | 1 cut(s) | 168 |
| BssECI | CCNNGG | 1 cut(s) | 248 |
| BssMI | GATC | 1 cut(s) | 160 |
| BssT1I | CCWWGG | 1 cut(s) | 248 |
| Bst4CI | ACNGT | 3 cut(s) | 115, 232, 304 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 106 |
| BstDEI | CTNAG | 2 cut(s) | 30, 429 |
| BstF5I | GGATG | 2 cut(s) | 331, 409 |
| BstKTI | GATC | 1 cut(s) | 163 |
| BstMAI | GTCTC | 1 cut(s) | 329 |
| BstMBI | GATC | 1 cut(s) | 160 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 106, 293 |
| BstNSI | RCATGY | 2 cut(s) | 170, 437 |
| BstV1I | GCAGC | 2 cut(s) | 157, 479 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 263 |
| BtsCI | GGATG | 2 cut(s) | 331, 409 |
| BveI | ACCTGC | 1 cut(s) | 461 |
| Cfr13I | GGNCC | 2 cut(s) | 208, 253 |
| Csp6I | GTAC | 1 cut(s) | 61 |
| CviAII | CATG | 6 cut(s) | 101, 119, 136, 167, 200, 434 |
| CviJI | RGCY | 6 cut(s) | 16, 34, 320, 414, 478, 498 |
| CviKI_1 | RGCY | 6 cut(s) | 16, 34, 320, 414, 478, 498 |
| CviQI | GTAC | 1 cut(s) | 61 |
| DdeI | CTNAG | 2 cut(s) | 30, 429 |
| DpnI | GATC | 1 cut(s) | 162 |
| DpnII | GATC | 1 cut(s) | 160 |
| Ecl136II | GAGCTC | 1 cut(s) | 414 |
| Eco130I | CCWWGG | 1 cut(s) | 248 |
| Eco24I | GRGCYC | 1 cut(s) | 416 |
| Eco47I | GGWCC | 2 cut(s) | 208, 253 |
| Eco53kI | GAGCTC | 1 cut(s) | 414 |
| Eco57I | CTGAAG | 1 cut(s) | 38 |
| Eco88I | CYCGRG | 1 cut(s) | 409 |
| EcoICRI | GAGCTC | 1 cut(s) | 414 |
| EcoT14I | CCWWGG | 1 cut(s) | 248 |
| EcoT38I | GRGCYC | 1 cut(s) | 416 |
| ErhI | CCWWGG | 1 cut(s) | 248 |
| FaeI | CATG | 6 cut(s) | 104, 122, 139, 170, 203, 437 |
| FaiI | YATR | 8 cut(s) | 102, 107, 120, 137, 168, 201, 227, 435 |
| FatI | CATG | 6 cut(s) | 100, 118, 135, 166, 199, 433 |
| Fnu4HI | GCNGC | 3 cut(s) | 171, 318, 468 |
| FokI | GGATG | 2 cut(s) | 338, 416 |
| FriOI | GRGCYC | 1 cut(s) | 416 |
| Fsp4HI | GCNGC | 3 cut(s) | 171, 318, 468 |
| FspBI | CTAG | 5 cut(s) | 180, 249, 458, 495, 499 |
| GluI | GCNGC | 3 cut(s) | 171, 318, 468 |
| GsaI | CCCAGC | 1 cut(s) | 349 |
| Hin1II | CATG | 6 cut(s) | 104, 122, 139, 170, 203, 437 |
| HinfI | GANTC | 2 cut(s) | 242, 407 |
| HphI | GGTGA | 1 cut(s) | 485 |
| Hpy188I | TCNGA | 1 cut(s) | 91 |
| Hpy188III | TCNNGA | 4 cut(s) | 56, 264, 440, 458 |
| HpyAV | CCTTC | 2 cut(s) | 151, 261 |
| HpyCH4III | ACNGT | 3 cut(s) | 115, 232, 304 |
| HpyCH4V | TGCA | 5 cut(s) | 109, 199, 296, 395, 467 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 106, 293 |
| HpyF3I | CTNAG | 2 cut(s) | 30, 429 |
| Hsp92II | CATG | 6 cut(s) | 104, 122, 139, 170, 203, 437 |
| Kzo9I | GATC | 1 cut(s) | 160 |
| LmnI | GCTCC | 3 cut(s) | 196, 419, 445 |
| LpnPI | CCDG | 6 cut(s) | 41, 207, 249, 359, 381, 456 |
| Lsp1109I | GCAGC | 2 cut(s) | 157, 479 |
| MaeI | CTAG | 5 cut(s) | 180, 249, 458, 495, 499 |
| MaeIII | GTNAC | 3 cut(s) | 82, 151, 385 |
| MalI | GATC | 1 cut(s) | 162 |
| MboI | GATC | 1 cut(s) | 160 |
| MboII | GAAGA | 2 cut(s) | 44, 252 |
| MhlI | GDGCHC | 1 cut(s) | 416 |
| MluCI | AATT | 4 cut(s) | 147, 272, 282, 290 |
| MlyI | GAGTC | 2 cut(s) | 236, 401 |
| MmeI | TCCRAC | 1 cut(s) | 245 |
| MnlI | CCTC | 4 cut(s) | 307, 394, 427, 436 |
| MseI | TTAA | 1 cut(s) | 309 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 106, 293 |
| NdeII | GATC | 1 cut(s) | 160 |
| NlaIII | CATG | 6 cut(s) | 104, 122, 139, 170, 203, 437 |
| NlaIV | GGNNCC | 1 cut(s) | 254 |
| NmuCI | GTSAC | 1 cut(s) | 82 |
| NspI | RCATGY | 2 cut(s) | 170, 437 |
| PaeR7I | CTCGAG | 1 cut(s) | 409 |
| PaqCI | CACCTGC | 1 cut(s) | 461 |
| PkrI | GCNGC | 3 cut(s) | 172, 319, 469 |
| PleI | GAGTC | 2 cut(s) | 236, 401 |
| PpsI | GAGTC | 2 cut(s) | 236, 401 |
| Psp124BI | GAGCTC | 1 cut(s) | 416 |
| PspFI | CCCAGC | 1 cut(s) | 345 |
| PspN4I | GGNNCC | 1 cut(s) | 254 |
| PspPI | GGNCC | 2 cut(s) | 208, 253 |
| PspXI | VCTCGAGB | 1 cut(s) | 409 |
| RsaI | GTAC | 1 cut(s) | 62 |
| RsaNI | GTAC | 1 cut(s) | 61 |
| SacI | GAGCTC | 1 cut(s) | 416 |
| SaqAI | TTAA | 1 cut(s) | 309 |
| SatI | GCNGC | 3 cut(s) | 171, 318, 468 |
| Sau3AI | GATC | 1 cut(s) | 160 |
| Sau96I | GGNCC | 2 cut(s) | 208, 253 |
| SchI | GAGTC | 2 cut(s) | 236, 401 |
| SduI | GDGCHC | 1 cut(s) | 416 |
| SetI | ASST | 6 cut(s) | 18, 69, 416, 475, 480, 500 |
| Sfr274I | CTCGAG | 1 cut(s) | 409 |
| SinI | GGWCC | 2 cut(s) | 208, 253 |
| SlaI | CTCGAG | 1 cut(s) | 409 |
| SmlI | CTYRAG | 4 cut(s) | 17, 409, 418, 440 |
| SmoI | CTYRAG | 4 cut(s) | 17, 409, 418, 440 |
| SpeI | ACTAGT | 1 cut(s) | 179 |
| Sse9I | AATT | 4 cut(s) | 147, 272, 282, 290 |
| SsiI | CCGC | 1 cut(s) | 317 |
| SspI | AATATT | 1 cut(s) | 379 |
| SspMI | CTAG | 5 cut(s) | 180, 249, 458, 495, 499 |
| SstI | GAGCTC | 1 cut(s) | 416 |
| StyI | CCWWGG | 1 cut(s) | 248 |
| TaaI | ACNGT | 3 cut(s) | 115, 232, 304 |
| TaqI | TCGA | 1 cut(s) | 410 |
| TasI | AATT | 4 cut(s) | 147, 272, 282, 290 |
| TatI | WGTACW | 1 cut(s) | 60 |
| TauI | GCSGC | 1 cut(s) | 320 |
| Tru1I | TTAA | 1 cut(s) | 309 |
| Tru9I | TTAA | 1 cut(s) | 309 |
| TseFI | GTSAC | 1 cut(s) | 82 |
| TseI | GCWGC | 2 cut(s) | 170, 467 |
| Tsp45I | GTSAC | 1 cut(s) | 82 |
| TspDTI | ATGAA | 1 cut(s) | 342 |
| VpaK11BI | GGWCC | 2 cut(s) | 208, 253 |
| XbaI | TCTAGA | 1 cut(s) | 457 |
| XceI | RCATGY | 2 cut(s) | 170, 437 |
| XhoI | CTCGAG | 1 cut(s) | 409 |
| XmaJI | CCTAGG | 1 cut(s) | 248 |
| XspI | CTAG | 5 cut(s) | 180, 249, 458, 495, 499 |