Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 684, 1111 |
| AatII | GACGTC | 1 cut(s) | 464 |
| Acc36I | ACCTGC | 1 cut(s) | 848 |
| AciI | CCGC | 3 cut(s) | 132, 400, 941 |
| AclWI | GGATC | 2 cut(s) | 319, 391 |
| AcoI | YGGCCR | 2 cut(s) | 93, 897 |
| AcuI | CTGAAG | 1 cut(s) | 510 |
| AcyI | GRCGYC | 1 cut(s) | 461 |
| AfaI | GTAC | 4 cut(s) | 59, 83, 754, 773 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 191, 232, 415, 659, 906, 1043 |
| AflIII | ACRYGT | 1 cut(s) | 78 |
| AgeI | ACCGGT | 1 cut(s) | 624 |
| AgsI | TTSAA | 2 cut(s) | 611, 619 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 636 |
| AjnI | CCWGG | 4 cut(s) | 414, 539, 899, 1052 |
| AleI | CACNNNNGTG | 3 cut(s) | 89, 593, 595 |
| Alw21I | GWGCWC | 2 cut(s) | 156, 853 |
| Alw26I | GTCTC | 3 cut(s) | 272, 822, 1090 |
| AlwI | GGATC | 2 cut(s) | 319, 391 |
| AoxI | GGCC | 5 cut(s) | 93, 129, 493, 511, 897 |
| ApeKI | GCWGC | 4 cut(s) | 569, 572, 953, 956 |
| AsiGI | ACCGGT | 1 cut(s) | 624 |
| AspS9I | GGNCC | 3 cut(s) | 494, 512, 622 |
| AsuHPI | GGTGA | 3 cut(s) | 135, 714, 1042 |
| AsuNHI | GCTAGC | 2 cut(s) | 584, 869 |
| AvaII | GGWCC | 1 cut(s) | 622 |
| AxyI | CCTNAGG | 1 cut(s) | 642 |
| BaeGI | GKGCMC | 1 cut(s) | 941 |
| BalI | TGGCCA | 2 cut(s) | 95, 899 |
| BanII | GRGCYC | 2 cut(s) | 156, 853 |
| Bbv12I | GWGCWC | 2 cut(s) | 156, 853 |
| BbvI | GCAGC | 4 cut(s) | 556, 559, 943, 965 |
| BccI | CCATC | 4 cut(s) | 148, 194, 290, 907 |
| BceAI | ACGGC | 2 cut(s) | 810, 923 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 444, 478 |
| BciT130I | CCWGG | 4 cut(s) | 416, 541, 901, 1054 |
| BcoDI | GTCTC | 3 cut(s) | 272, 822, 1090 |
| BfaI | CTAG | 4 cut(s) | 291, 585, 654, 870 |
| BfmI | CTRYAG | 2 cut(s) | 748, 1000 |
| BfuAI | ACCTGC | 1 cut(s) | 848 |
| BisI | GCNGC | 5 cut(s) | 132, 570, 573, 954, 957 |
| BlsI | GCNGC | 5 cut(s) | 133, 571, 574, 955, 958 |
| Bme1390I | CCNGG | 4 cut(s) | 416, 541, 901, 1054 |
| Bme18I | GGWCC | 1 cut(s) | 622 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 636 |
| BmgT120I | GGNCC | 3 cut(s) | 494, 512, 622 |
| BmrFI | CCNGG | 4 cut(s) | 416, 541, 901, 1054 |
| BmsI | GCATC | 2 cut(s) | 729, 1056 |
| BmtI | GCTAGC | 2 cut(s) | 588, 873 |
| BpuEI | CTTGAG | 3 cut(s) | 134, 220, 296 |
| BsaHI | GRCGYC | 1 cut(s) | 461 |
| BsaI | GGTCTC | 1 cut(s) | 272 |
| BsaJI | CCNNGG | 1 cut(s) | 414 |
| BsaWI | WCCGGW | 1 cut(s) | 624 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 8, 38, 41, 71 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 191, 232, 415, 659, 906, 1043 |
| Bse118I | RCCGGY | 1 cut(s) | 624 |
| Bse1I | ACTGG | 2 cut(s) | 87, 846 |
| Bse21I | CCTNAGG | 1 cut(s) | 642 |
| Bse3DI | GCAATG | 2 cut(s) | 255, 462 |
| BseBI | CCWGG | 4 cut(s) | 416, 541, 901, 1054 |
| BseDI | CCNNGG | 1 cut(s) | 414 |
| BseGI | GGATG | 4 cut(s) | 601, 744, 1062, 1102 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 191, 232, 415, 659, 906, 1043 |
| BseMI | GCAATG | 2 cut(s) | 255, 462 |
| BseMII | CTCAG | 2 cut(s) | 47, 633 |
| BseNI | ACTGG | 2 cut(s) | 87, 846 |
| BseRI | GAGGAG | 1 cut(s) | 261 |
| BseSI | GKGCMC | 1 cut(s) | 941 |
| BseXI | GCAGC | 4 cut(s) | 556, 559, 943, 965 |
| BsgI | GTGCAG | 1 cut(s) | 942 |
| BshFI | GGCC | 5 cut(s) | 95, 131, 495, 513, 899 |
| BshTI | ACCGGT | 1 cut(s) | 624 |
| BsiHKAI | GWGCWC | 2 cut(s) | 156, 853 |
| BsiSI | CCGG | 1 cut(s) | 625 |
| BslFI | GGGAC | 4 cut(s) | 89, 392, 445, 1008 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 191, 232, 415, 659, 906, 1043 |
| BsmAI | GTCTC | 3 cut(s) | 272, 822, 1090 |
| BsmFI | GGGAC | 4 cut(s) | 89, 392, 445, 1008 |
| BsnI | GGCC | 5 cut(s) | 95, 131, 495, 513, 899 |
| Bso31I | GGTCTC | 1 cut(s) | 272 |
| Bsp1286I | GDGCHC | 3 cut(s) | 156, 853, 941 |
| Bsp1407I | TGTACA | 1 cut(s) | 81 |
| Bsp143I | GATC | 2 cut(s) | 311, 396 |
| BspACI | CCGC | 3 cut(s) | 132, 400, 941 |
| BspANI | GGCC | 5 cut(s) | 95, 131, 495, 513, 899 |
| BspCNI | CTCAG | 2 cut(s) | 46, 634 |
| BspMI | ACCTGC | 1 cut(s) | 848 |
| BspOI | GCTAGC | 2 cut(s) | 588, 873 |
| BspPI | GGATC | 2 cut(s) | 319, 391 |
| BspTNI | GGTCTC | 1 cut(s) | 272 |
| BsrDI | GCAATG | 2 cut(s) | 255, 462 |
| BsrFI | RCCGGY | 1 cut(s) | 624 |
| BsrGI | TGTACA | 1 cut(s) | 81 |
| BsrI | ACTGG | 2 cut(s) | 87, 846 |
| BssAI | RCCGGY | 1 cut(s) | 624 |
| BssECI | CCNNGG | 1 cut(s) | 414 |
| BssMI | GATC | 2 cut(s) | 311, 396 |
| BssNI | GRCGYC | 1 cut(s) | 461 |
| Bst2UI | CCWGG | 4 cut(s) | 416, 541, 901, 1054 |
| Bst4CI | ACNGT | 3 cut(s) | 62, 634, 752 |
| BstACI | GRCGYC | 1 cut(s) | 461 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 383 |
| BstAUI | TGTACA | 1 cut(s) | 81 |
| BstC8I | GCNNGC | 4 cut(s) | 586, 871, 941, 1018 |
| BstDEI | CTNAG | 5 cut(s) | 33, 227, 337, 642, 821 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 657 |
| BstF5I | GGATG | 4 cut(s) | 601, 744, 1062, 1102 |
| BstKTI | GATC | 2 cut(s) | 314, 399 |
| BstMAI | GTCTC | 3 cut(s) | 272, 822, 1090 |
| BstMBI | GATC | 2 cut(s) | 311, 396 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 266, 361, 383, 519, 569, 581, 879 |
| BstNI | CCWGG | 4 cut(s) | 416, 541, 901, 1054 |
| BstNSI | RCATGY | 2 cut(s) | 82, 759 |
| BstSCI | CCNGG | 4 cut(s) | 414, 539, 899, 1052 |
| BstSFI | CTRYAG | 2 cut(s) | 748, 1000 |
| BstSLI | GKGCMC | 1 cut(s) | 941 |
| BstV1I | GCAGC | 4 cut(s) | 556, 559, 943, 965 |
| Bsu36I | CCTNAGG | 1 cut(s) | 642 |
| BsuRI | GGCC | 5 cut(s) | 95, 131, 495, 513, 899 |
| BtsCI | GGATG | 4 cut(s) | 601, 744, 1062, 1102 |
| BtsIMutI | CAGTG | 3 cut(s) | 67, 94, 728 |
| BveI | ACCTGC | 1 cut(s) | 848 |
| Cac8I | GCNNGC | 4 cut(s) | 586, 871, 941, 1018 |
| Cfr10I | RCCGGY | 1 cut(s) | 624 |
| Cfr13I | GGNCC | 3 cut(s) | 494, 512, 622 |
| Csp6I | GTAC | 4 cut(s) | 58, 82, 753, 772 |
| CspAI | ACCGGT | 1 cut(s) | 624 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 353, 388, 458, 493 |
| CviAII | CATG | 5 cut(s) | 79, 705, 756, 769, 1033 |
| CviQI | GTAC | 4 cut(s) | 58, 82, 753, 772 |
| DdeI | CTNAG | 5 cut(s) | 33, 227, 337, 642, 821 |
| DpnI | GATC | 2 cut(s) | 313, 398 |
| DpnII | GATC | 2 cut(s) | 311, 396 |
| DraI | TTTAAA | 1 cut(s) | 1026 |
| DriI | GACNNNNNGTC | 1 cut(s) | 636 |
| EaeI | YGGCCR | 2 cut(s) | 93, 897 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 636 |
| Ecl136II | GAGCTC | 2 cut(s) | 154, 851 |
| Eco24I | GRGCYC | 2 cut(s) | 156, 853 |
| Eco31I | GGTCTC | 1 cut(s) | 272 |
| Eco47I | GGWCC | 1 cut(s) | 622 |
| Eco53kI | GAGCTC | 2 cut(s) | 154, 851 |
| Eco57I | CTGAAG | 1 cut(s) | 510 |
| Eco81I | CCTNAGG | 1 cut(s) | 642 |
| EcoICRI | GAGCTC | 2 cut(s) | 154, 851 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 657 |
| EcoRII | CCWGG | 4 cut(s) | 414, 539, 899, 1052 |
| EcoT38I | GRGCYC | 2 cut(s) | 156, 853 |
| FaeI | CATG | 5 cut(s) | 82, 708, 759, 772, 1036 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 249, 281 |
| FaqI | GGGAC | 4 cut(s) | 89, 392, 445, 1008 |
| FatI | CATG | 5 cut(s) | 78, 704, 755, 768, 1032 |
| FauI | CCCGC | 1 cut(s) | 948 |
| FauNDI | CATATG | 1 cut(s) | 948 |
| Fnu4HI | GCNGC | 5 cut(s) | 132, 570, 573, 954, 957 |
| FokI | GGATG | 4 cut(s) | 588, 751, 1069, 1089 |
| FriOI | GRGCYC | 2 cut(s) | 156, 853 |
| Fsp4HI | GCNGC | 5 cut(s) | 132, 570, 573, 954, 957 |
| FspBI | CTAG | 4 cut(s) | 291, 585, 654, 870 |
| GluI | GCNGC | 5 cut(s) | 132, 570, 573, 954, 957 |
| HaeIII | GGCC | 5 cut(s) | 95, 131, 495, 513, 899 |
| HapII | CCGG | 1 cut(s) | 625 |
| Hin1I | GRCGYC | 1 cut(s) | 461 |
| Hin1II | CATG | 5 cut(s) | 82, 708, 759, 772, 1036 |
| HincII | GTYRAC | 1 cut(s) | 630 |
| HindII | GTYRAC | 1 cut(s) | 630 |
| HindIII | AAGCTT | 2 cut(s) | 520, 1068 |
| HinfI | GANTC | 3 cut(s) | 195, 815, 996 |
| HpaII | CCGG | 1 cut(s) | 625 |
| HphI | GGTGA | 3 cut(s) | 135, 714, 1042 |
| Hpy166II | GTNNAC | 2 cut(s) | 84, 630 |
| Hpy188I | TCNGA | 4 cut(s) | 191, 848, 967, 1095 |
| Hpy188III | TCNNGA | 7 cut(s) | 199, 275, 286, 315, 641, 784, 830 |
| Hpy8I | GTNNAC | 2 cut(s) | 84, 630 |
| Hpy99I | CGWCG | 1 cut(s) | 466 |
| HpyAV | CCTTC | 6 cut(s) | 40, 485, 613, 683, 977, 1047 |
| HpyCH4III | ACNGT | 3 cut(s) | 62, 634, 752 |
| HpyCH4IV | ACGT | 2 cut(s) | 434, 461 |
| HpyCH4V | TGCA | 6 cut(s) | 386, 505, 668, 928, 959, 1016 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 266, 361, 383, 519, 569, 581, 879 |
| HpyF3I | CTNAG | 5 cut(s) | 33, 227, 337, 642, 821 |
| HpySE526I | ACGT | 2 cut(s) | 434, 461 |
| Hsp92I | GRCGYC | 1 cut(s) | 461 |
| Hsp92II | CATG | 5 cut(s) | 82, 708, 759, 772, 1036 |
| Kzo9I | GATC | 2 cut(s) | 311, 396 |
| LmnI | GCTCC | 4 cut(s) | 159, 274, 848, 920 |
| Lsp1109I | GCAGC | 4 cut(s) | 556, 559, 943, 965 |
| LweI | GCATC | 2 cut(s) | 729, 1056 |
| MaeI | CTAG | 4 cut(s) | 291, 585, 654, 870 |
| MaeII | ACGT | 2 cut(s) | 434, 461 |
| MaeIII | GTNAC | 2 cut(s) | 799, 837 |
| MalI | GATC | 2 cut(s) | 313, 398 |
| MboI | GATC | 2 cut(s) | 311, 396 |
| MboII | GAAGA | 2 cut(s) | 55, 64 |
| MhlI | GDGCHC | 3 cut(s) | 156, 853, 941 |
| MlsI | TGGCCA | 2 cut(s) | 95, 899 |
| MluCI | AATT | 3 cut(s) | 164, 507, 579 |
| MluNI | TGGCCA | 2 cut(s) | 95, 899 |
| MlyI | GAGTC | 2 cut(s) | 824, 990 |
| MmeI | TCCRAC | 4 cut(s) | 196, 289, 826, 898 |
| Mox20I | TGGCCA | 2 cut(s) | 95, 899 |
| MscI | TGGCCA | 2 cut(s) | 95, 899 |
| MseI | TTAA | 2 cut(s) | 447, 1025 |
| MslI | CAYNNNNRTG | 4 cut(s) | 89, 593, 595, 1055 |
| Msp20I | TGGCCA | 2 cut(s) | 95, 899 |
| MspA1I | CMGCKG | 1 cut(s) | 956 |
| MspI | CCGG | 1 cut(s) | 625 |
| MspR9I | CCNGG | 4 cut(s) | 416, 541, 901, 1054 |
| MvaI | CCWGG | 4 cut(s) | 416, 541, 901, 1054 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 266, 361, 383, 519, 569, 581, 879 |
| NdeI | CATATG | 1 cut(s) | 948 |
| NdeII | GATC | 2 cut(s) | 311, 396 |
| NheI | GCTAGC | 2 cut(s) | 584, 869 |
| NlaIII | CATG | 5 cut(s) | 82, 708, 759, 772, 1036 |
| NspI | RCATGY | 2 cut(s) | 82, 759 |
| OliI | CACNNNNGTG | 3 cut(s) | 89, 593, 595 |
| PciI | ACATGT | 1 cut(s) | 78 |
| PfeI | GAWTC | 1 cut(s) | 195 |
| PfoI | TCCNGGA | 1 cut(s) | 539 |
| PinAI | ACCGGT | 1 cut(s) | 624 |
| PkrI | GCNGC | 5 cut(s) | 133, 571, 574, 955, 958 |
| PleI | GAGTC | 2 cut(s) | 823, 990 |
| PpsI | GAGTC | 2 cut(s) | 823, 990 |
| PscI | ACATGT | 1 cut(s) | 78 |
| PsiI | TTATAA | 2 cut(s) | 684, 1111 |
| Psp124BI | GAGCTC | 2 cut(s) | 156, 853 |
| Psp6I | CCWGG | 4 cut(s) | 414, 539, 899, 1052 |
| PspGI | CCWGG | 4 cut(s) | 414, 539, 899, 1052 |
| PspPI | GGNCC | 3 cut(s) | 494, 512, 622 |
| PvuII | CAGCTG | 1 cut(s) | 956 |
| RsaI | GTAC | 4 cut(s) | 59, 83, 754, 773 |
| RsaNI | GTAC | 4 cut(s) | 58, 82, 753, 772 |
| RseI | CAYNNNNRTG | 4 cut(s) | 89, 593, 595, 1055 |
| SacI | GAGCTC | 2 cut(s) | 156, 853 |
| SaqAI | TTAA | 2 cut(s) | 447, 1025 |
| SatI | GCNGC | 5 cut(s) | 132, 570, 573, 954, 957 |
| Sau3AI | GATC | 2 cut(s) | 311, 396 |
| Sau96I | GGNCC | 3 cut(s) | 494, 512, 622 |
| SchI | GAGTC | 2 cut(s) | 824, 990 |
| ScrFI | CCNGG | 4 cut(s) | 416, 541, 901, 1054 |
| SduI | GDGCHC | 3 cut(s) | 156, 853, 941 |
| SfaNI | GCATC | 2 cut(s) | 729, 1056 |
| SfcI | CTRYAG | 2 cut(s) | 748, 1000 |
| SinI | GGWCC | 1 cut(s) | 622 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 89, 593, 595, 1055 |
| SmlI | CTYRAG | 3 cut(s) | 113, 199, 275 |
| SmoI | CTYRAG | 3 cut(s) | 113, 199, 275 |
| Sse9I | AATT | 3 cut(s) | 164, 507, 579 |
| SsiI | CCGC | 3 cut(s) | 132, 400, 941 |
| SspMI | CTAG | 4 cut(s) | 291, 585, 654, 870 |
| SstI | GAGCTC | 2 cut(s) | 156, 853 |
| StyD4I | CCNGG | 4 cut(s) | 414, 539, 899, 1052 |
| TaaI | ACNGT | 3 cut(s) | 62, 634, 752 |
| TaiI | ACGT | 2 cut(s) | 437, 464 |
| TasI | AATT | 3 cut(s) | 164, 507, 579 |
| TatI | WGTACW | 3 cut(s) | 57, 81, 752 |
| TauI | GCSGC | 1 cut(s) | 134 |
| TfiI | GAWTC | 1 cut(s) | 195 |
| Tru1I | TTAA | 2 cut(s) | 447, 1025 |
| Tru9I | TTAA | 2 cut(s) | 447, 1025 |
| TscAI | CASTG | 3 cut(s) | 67, 94, 735 |
| TseI | GCWGC | 4 cut(s) | 569, 572, 953, 956 |
| TspDTI | ATGAA | 5 cut(s) | 56, 693, 757, 1029, 1080 |
| TspRI | CASTG | 3 cut(s) | 67, 94, 735 |
| VpaK11BI | GGWCC | 1 cut(s) | 622 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 657 |
| XceI | RCATGY | 2 cut(s) | 82, 759 |
| XspI | CTAG | 4 cut(s) | 291, 585, 654, 870 |
| ZraI | GACGTC | 1 cut(s) | 462 |