Reverse transcriptase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 385 |
| AccII | CGCG | 1 cut(s) | 667 |
| AclI | AACGTT | 1 cut(s) | 302 |
| AcsI | RAATTY | 1 cut(s) | 247 |
| AfaI | GTAC | 1 cut(s) | 163 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 622 |
| AflII | CTTAAG | 1 cut(s) | 617 |
| AflIII | ACRYGT | 1 cut(s) | 158 |
| AgsI | TTSAA | 3 cut(s) | 284, 578, 644 |
| AhlI | ACTAGT | 1 cut(s) | 332 |
| AjnI | CCWGG | 2 cut(s) | 257, 440 |
| AluBI | AGCT | 4 cut(s) | 287, 348, 584, 647 |
| AluI | AGCT | 4 cut(s) | 287, 348, 584, 647 |
| Alw26I | GTCTC | 1 cut(s) | 200 |
| Ama87I | CYCGRG | 1 cut(s) | 377 |
| AoxI | GGCC | 1 cut(s) | 81 |
| ApeKI | GCWGC | 1 cut(s) | 8 |
| ApoI | RAATTY | 1 cut(s) | 247 |
| AseI | ATTAAT | 1 cut(s) | 318 |
| AspLEI | GCGC | 4 cut(s) | 69, 418, 667, 669 |
| AspS9I | GGNCC | 1 cut(s) | 255 |
| AsuC2I | CCSGG | 2 cut(s) | 378, 379 |
| AsuHPI | GGTGA | 4 cut(s) | 232, 420, 470, 511 |
| AvaI | CYCGRG | 1 cut(s) | 377 |
| AvaII | GGWCC | 1 cut(s) | 255 |
| BaeGI | GKGCMC | 1 cut(s) | 616 |
| BanI | GGYRCC | 1 cut(s) | 385 |
| BanII | GRGCYC | 1 cut(s) | 448 |
| BbsI | GAAGAC | 1 cut(s) | 95 |
| BbvI | GCAGC | 1 cut(s) | 20 |
| BccI | CCATC | 1 cut(s) | 376 |
| BceAI | ACGGC | 3 cut(s) | 76, 429, 620 |
| BciT130I | CCWGG | 2 cut(s) | 259, 442 |
| BcnI | CCSGG | 2 cut(s) | 378, 379 |
| BcoDI | GTCTC | 1 cut(s) | 200 |
| BcuI | ACTAGT | 1 cut(s) | 332 |
| BfaI | CTAG | 6 cut(s) | 198, 333, 461, 521, 602, 685 |
| BfmI | CTRYAG | 1 cut(s) | 351 |
| BfoI | RGCGCY | 1 cut(s) | 419 |
| BfrI | CTTAAG | 1 cut(s) | 617 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| Bme1390I | CCNGG | 4 cut(s) | 259, 378, 379, 442 |
| Bme18I | GGWCC | 1 cut(s) | 255 |
| BmeT110I | CYCGRG | 1 cut(s) | 377 |
| BmgT120I | GGNCC | 1 cut(s) | 255 |
| BmiI | GGNNCC | 2 cut(s) | 387, 447 |
| BmrFI | CCNGG | 4 cut(s) | 259, 378, 379, 442 |
| BmsI | GCATC | 2 cut(s) | 7, 226 |
| BpiI | GAAGAC | 1 cut(s) | 95 |
| BpuMI | CCSGG | 2 cut(s) | 378, 379 |
| BsaAI | YACGTR | 2 cut(s) | 125, 191 |
| BsaBI | GATNNNNATC | 1 cut(s) | 528 |
| BsaJI | CCNNGG | 4 cut(s) | 92, 377, 441, 488 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 622 |
| Bse1I | ACTGG | 1 cut(s) | 670 |
| Bse8I | GATNNNNATC | 1 cut(s) | 528 |
| BseBI | CCWGG | 2 cut(s) | 259, 442 |
| BseDI | CCNNGG | 4 cut(s) | 92, 377, 441, 488 |
| BseGI | GGATG | 4 cut(s) | 43, 134, 148, 387 |
| BseJI | GATNNNNATC | 1 cut(s) | 528 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 622 |
| BseNI | ACTGG | 1 cut(s) | 670 |
| BsePI | GCGCGC | 1 cut(s) | 665 |
| BseRI | GAGGAG | 2 cut(s) | 279, 282 |
| BseSI | GKGCMC | 1 cut(s) | 616 |
| BseXI | GCAGC | 1 cut(s) | 20 |
| Bsh1236I | CGCG | 1 cut(s) | 667 |
| BshFI | GGCC | 1 cut(s) | 83 |
| BshNI | GGYRCC | 1 cut(s) | 385 |
| BsiHKCI | CYCGRG | 1 cut(s) | 377 |
| BsiSI | CCGG | 1 cut(s) | 378 |
| BslFI | GGGAC | 1 cut(s) | 89 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 622 |
| BsmAI | GTCTC | 1 cut(s) | 200 |
| BsmBI | CGTCTC | 1 cut(s) | 200 |
| BsmFI | GGGAC | 1 cut(s) | 89 |
| BsnI | GGCC | 1 cut(s) | 83 |
| BsoBI | CYCGRG | 1 cut(s) | 377 |
| Bsp1286I | GDGCHC | 2 cut(s) | 448, 616 |
| Bsp19I | CCATGG | 1 cut(s) | 488 |
| BspANI | GGCC | 1 cut(s) | 83 |
| BspFNI | CGCG | 1 cut(s) | 667 |
| BspLI | GGNNCC | 2 cut(s) | 387, 447 |
| BspT107I | GGYRCC | 1 cut(s) | 385 |
| BspTI | CTTAAG | 1 cut(s) | 617 |
| BsrI | ACTGG | 1 cut(s) | 670 |
| BssECI | CCNNGG | 4 cut(s) | 92, 377, 441, 488 |
| BssHII | GCGCGC | 1 cut(s) | 665 |
| BssT1I | CCWWGG | 1 cut(s) | 488 |
| Bst2UI | CCWGG | 2 cut(s) | 259, 442 |
| Bst4CI | ACNGT | 2 cut(s) | 426, 569 |
| Bst6I | CTCTTC | 1 cut(s) | 92 |
| BstAFI | CTTAAG | 1 cut(s) | 617 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 17 |
| BstBAI | YACGTR | 2 cut(s) | 125, 191 |
| BstC8I | GCNNGC | 4 cut(s) | 65, 309, 538, 667 |
| BstDSI | CCRYGG | 2 cut(s) | 92, 488 |
| BstF5I | GGATG | 4 cut(s) | 43, 134, 148, 387 |
| BstFNI | CGCG | 1 cut(s) | 667 |
| BstH2I | RGCGCY | 1 cut(s) | 419 |
| BstHHI | GCGC | 4 cut(s) | 69, 418, 667, 669 |
| BstMAI | GTCTC | 1 cut(s) | 200 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 17, 26, 546, 611 |
| BstNI | CCWGG | 2 cut(s) | 259, 442 |
| BstNSI | RCATGY | 2 cut(s) | 162, 311 |
| BstSCI | CCNGG | 4 cut(s) | 257, 376, 377, 440 |
| BstSFI | CTRYAG | 1 cut(s) | 351 |
| BstSLI | GKGCMC | 1 cut(s) | 616 |
| BstUI | CGCG | 1 cut(s) | 667 |
| BstV1I | GCAGC | 1 cut(s) | 20 |
| BstV2I | GAAGAC | 1 cut(s) | 95 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 57 |
| BsuRI | GGCC | 1 cut(s) | 83 |
| BtgI | CCRYGG | 2 cut(s) | 92, 488 |
| BtsCI | GGATG | 4 cut(s) | 43, 134, 148, 387 |
| BtsIMutI | CAGTG | 1 cut(s) | 677 |
| Cac8I | GCNNGC | 4 cut(s) | 65, 309, 538, 667 |
| CfoI | GCGC | 4 cut(s) | 69, 418, 667, 669 |
| Cfr13I | GGNCC | 1 cut(s) | 255 |
| Cfr9I | CCCGGG | 1 cut(s) | 377 |
| CseI | GACGC | 1 cut(s) | 162 |
| CsiI | ACCWGGT | 1 cut(s) | 257 |
| Csp6I | GTAC | 1 cut(s) | 162 |
| CviAII | CATG | 6 cut(s) | 159, 244, 265, 308, 489, 533 |
| CviQI | GTAC | 1 cut(s) | 162 |
| Eam1104I | CTCTTC | 1 cut(s) | 92 |
| EarI | CTCTTC | 1 cut(s) | 92 |
| Eco130I | CCWWGG | 1 cut(s) | 488 |
| Eco24I | GRGCYC | 1 cut(s) | 448 |
| Eco47I | GGWCC | 1 cut(s) | 255 |
| Eco88I | CYCGRG | 1 cut(s) | 377 |
| EcoRII | CCWGG | 2 cut(s) | 257, 440 |
| EcoT14I | CCWWGG | 1 cut(s) | 488 |
| EcoT38I | GRGCYC | 1 cut(s) | 448 |
| ErhI | CCWWGG | 1 cut(s) | 488 |
| Esp3I | CGTCTC | 1 cut(s) | 200 |
| FaeI | CATG | 6 cut(s) | 162, 247, 268, 311, 492, 536 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 36 |
| FaqI | GGGAC | 1 cut(s) | 89 |
| FatI | CATG | 6 cut(s) | 158, 243, 264, 307, 488, 532 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| FokI | GGATG | 4 cut(s) | 30, 141, 155, 394 |
| FriOI | GRGCYC | 1 cut(s) | 448 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 6 cut(s) | 198, 333, 461, 521, 602, 685 |
| GlaI | GCGC | 4 cut(s) | 68, 417, 666, 668 |
| GluI | GCNGC | 1 cut(s) | 9 |
| HaeII | RGCGCY | 1 cut(s) | 419 |
| HaeIII | GGCC | 1 cut(s) | 83 |
| HapII | CCGG | 1 cut(s) | 378 |
| HgaI | GACGC | 1 cut(s) | 162 |
| HhaI | GCGC | 4 cut(s) | 69, 418, 667, 669 |
| Hin1II | CATG | 6 cut(s) | 162, 247, 268, 311, 492, 536 |
| Hin6I | GCGC | 4 cut(s) | 67, 416, 665, 667 |
| HinP1I | GCGC | 4 cut(s) | 67, 416, 665, 667 |
| HinfI | GANTC | 2 cut(s) | 100, 676 |
| HpaII | CCGG | 1 cut(s) | 378 |
| HphI | GGTGA | 4 cut(s) | 232, 420, 470, 511 |
| Hpy166II | GTNNAC | 1 cut(s) | 502 |
| Hpy8I | GTNNAC | 1 cut(s) | 502 |
| Hpy99I | CGWCG | 1 cut(s) | 156 |
| HpyAV | CCTTC | 1 cut(s) | 109 |
| HpyCH4III | ACNGT | 2 cut(s) | 426, 569 |
| HpyCH4IV | ACGT | 4 cut(s) | 124, 190, 302, 475 |
| HpyCH4V | TGCA | 3 cut(s) | 264, 307, 536 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 17, 26, 546, 611 |
| HpySE526I | ACGT | 4 cut(s) | 124, 190, 302, 475 |
| Hsp92II | CATG | 6 cut(s) | 162, 247, 268, 311, 492, 536 |
| HspAI | GCGC | 4 cut(s) | 67, 416, 665, 667 |
| LmnI | GCTCC | 2 cut(s) | 292, 451 |
| LpnPI | CCDG | 8 cut(s) | 122, 244, 271, 391, 427, 454, 522, 683 |
| Lsp1109I | GCAGC | 1 cut(s) | 20 |
| LweI | GCATC | 2 cut(s) | 7, 226 |
| MabI | ACCWGGT | 1 cut(s) | 257 |
| MaeI | CTAG | 6 cut(s) | 198, 333, 461, 521, 602, 685 |
| MaeII | ACGT | 4 cut(s) | 124, 190, 302, 475 |
| MaeIII | GTNAC | 3 cut(s) | 238, 426, 476 |
| MboII | GAAGA | 3 cut(s) | 95, 109, 580 |
| MhlI | GDGCHC | 2 cut(s) | 448, 616 |
| MluCI | AATT | 5 cut(s) | 247, 296, 319, 639, 654 |
| MlyI | GAGTC | 1 cut(s) | 109 |
| MmeI | TCCRAC | 1 cut(s) | 449 |
| MnlI | CCTC | 4 cut(s) | 19, 57, 300, 303 |
| MseI | TTAA | 6 cut(s) | 299, 318, 372, 434, 618, 651 |
| MslI | CAYNNNNRTG | 1 cut(s) | 55 |
| MspCI | CTTAAG | 1 cut(s) | 617 |
| MspI | CCGG | 1 cut(s) | 378 |
| MspR9I | CCNGG | 4 cut(s) | 259, 378, 379, 442 |
| MvaI | CCWGG | 2 cut(s) | 259, 442 |
| MvnI | CGCG | 1 cut(s) | 667 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 17, 26, 546, 611 |
| NciI | CCSGG | 2 cut(s) | 378, 379 |
| NcoI | CCATGG | 1 cut(s) | 488 |
| NlaIII | CATG | 6 cut(s) | 162, 247, 268, 311, 492, 536 |
| NlaIV | GGNNCC | 2 cut(s) | 387, 447 |
| NmuCI | GTSAC | 3 cut(s) | 238, 426, 476 |
| NspI | RCATGY | 2 cut(s) | 162, 311 |
| PaeI | GCATGC | 1 cut(s) | 311 |
| PauI | GCGCGC | 1 cut(s) | 665 |
| PciI | ACATGT | 1 cut(s) | 158 |
| PfeI | GAWTC | 1 cut(s) | 676 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 108 |
| PpsI | GAGTC | 1 cut(s) | 108 |
| Ppu21I | YACGTR | 2 cut(s) | 125, 191 |
| PscI | ACATGT | 1 cut(s) | 158 |
| PshBI | ATTAAT | 1 cut(s) | 318 |
| Psp1406I | AACGTT | 1 cut(s) | 302 |
| Psp6I | CCWGG | 2 cut(s) | 257, 440 |
| PspGI | CCWGG | 2 cut(s) | 257, 440 |
| PspN4I | GGNNCC | 2 cut(s) | 387, 447 |
| PspPI | GGNCC | 1 cut(s) | 255 |
| PteI | GCGCGC | 1 cut(s) | 665 |
| RsaI | GTAC | 1 cut(s) | 163 |
| RsaNI | GTAC | 1 cut(s) | 162 |
| RseI | CAYNNNNRTG | 1 cut(s) | 55 |
| SaqAI | TTAA | 6 cut(s) | 299, 318, 372, 434, 618, 651 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau96I | GGNCC | 1 cut(s) | 255 |
| SchI | GAGTC | 1 cut(s) | 109 |
| ScrFI | CCNGG | 4 cut(s) | 259, 378, 379, 442 |
| SduI | GDGCHC | 2 cut(s) | 448, 616 |
| SexAI | ACCWGGT | 1 cut(s) | 257 |
| SfaNI | GCATC | 2 cut(s) | 7, 226 |
| SfcI | CTRYAG | 1 cut(s) | 351 |
| SinI | GGWCC | 1 cut(s) | 255 |
| SmaI | CCCGGG | 1 cut(s) | 379 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 55 |
| SmlI | CTYRAG | 1 cut(s) | 617 |
| SmoI | CTYRAG | 1 cut(s) | 617 |
| SpeI | ACTAGT | 1 cut(s) | 332 |
| SphI | GCATGC | 1 cut(s) | 311 |
| Sse9I | AATT | 5 cut(s) | 247, 296, 319, 639, 654 |
| SspMI | CTAG | 6 cut(s) | 198, 333, 461, 521, 602, 685 |
| StyD4I | CCNGG | 4 cut(s) | 257, 376, 377, 440 |
| StyI | CCWWGG | 1 cut(s) | 488 |
| TaaI | ACNGT | 2 cut(s) | 426, 569 |
| TaiI | ACGT | 4 cut(s) | 127, 193, 305, 478 |
| TasI | AATT | 5 cut(s) | 247, 296, 319, 639, 654 |
| TatI | WGTACW | 1 cut(s) | 161 |
| TfiI | GAWTC | 1 cut(s) | 676 |
| Tru1I | TTAA | 6 cut(s) | 299, 318, 372, 434, 618, 651 |
| Tru9I | TTAA | 6 cut(s) | 299, 318, 372, 434, 618, 651 |
| TscAI | CASTG | 1 cut(s) | 677 |
| TseFI | GTSAC | 3 cut(s) | 238, 426, 476 |
| TseI | GCWGC | 1 cut(s) | 8 |
| Tsp45I | GTSAC | 3 cut(s) | 238, 426, 476 |
| TspDTI | ATGAA | 4 cut(s) | 260, 350, 445, 540 |
| TspMI | CCCGGG | 1 cut(s) | 377 |
| TspRI | CASTG | 1 cut(s) | 677 |
| Vha464I | CTTAAG | 1 cut(s) | 617 |
| VpaK11BI | GGWCC | 1 cut(s) | 255 |
| VspI | ATTAAT | 1 cut(s) | 318 |
| XapI | RAATTY | 1 cut(s) | 247 |
| XceI | RCATGY | 2 cut(s) | 162, 311 |
| XmaI | CCCGGG | 1 cut(s) | 377 |
| XspI | CTAG | 6 cut(s) | 198, 333, 461, 521, 602, 685 |