The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 63 |
| AarI | CACCTGC | 1 cut(s) | 1576 |
| Acc36I | ACCTGC | 3 cut(s) | 936, 1216, 1576 |
| AccB1I | GGYRCC | 1 cut(s) | 1879 |
| AccI | GTMKAC | 1 cut(s) | 1636 |
| AciI | CCGC | 6 cut(s) | 796, 1086, 1597, 2507, 2543, 2636 |
| AclWI | GGATC | 6 cut(s) | 1024, 1647, 1660, 1873, 2180, 2193 |
| AcoI | YGGCCR | 1 cut(s) | 1956 |
| AcsI | RAATTY | 4 cut(s) | 710, 1131, 1236, 1975 |
| AcuI | CTGAAG | 1 cut(s) | 2257 |
| AdeI | CACNNNGTG | 1 cut(s) | 1733 |
| AfaI | GTAC | 5 cut(s) | 871, 1507, 1605, 1622, 1996 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1827, 1868, 2219, 2394, 2395 |
| AgsI | TTSAA | 4 cut(s) | 805, 1673, 1850, 2534 |
| AjiI | CACGTC | 2 cut(s) | 1414, 2605 |
| AjnI | CCWGG | 5 cut(s) | 1171, 1665, 2181, 2233, 2424 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 165, 197, 2182, 2214 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 7, 39, 1805, 1837 |
| AluBI | AGCT | 8 cut(s) | 115, 180, 464, 496, 1081, 1856, 2462, 2654 |
| AluI | AGCT | 8 cut(s) | 115, 180, 464, 496, 1081, 1856, 2462, 2654 |
| Alw21I | GWGCWC | 1 cut(s) | 2481 |
| Alw26I | GTCTC | 2 cut(s) | 1247, 1404 |
| AlwI | GGATC | 6 cut(s) | 1024, 1647, 1660, 1873, 2180, 2193 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1400, 1430 |
| Ama87I | CYCGRG | 2 cut(s) | 1861, 2388 |
| AoxI | GGCC | 4 cut(s) | 959, 1956, 2231, 2404 |
| ApeKI | GCWGC | 4 cut(s) | 1078, 1287, 1344, 2462 |
| ApoI | RAATTY | 4 cut(s) | 710, 1131, 1236, 1975 |
| AseI | ATTAAT | 1 cut(s) | 2537 |
| AspS9I | GGNCC | 2 cut(s) | 2261, 2318 |
| AsuC2I | CCSGG | 3 cut(s) | 1731, 1862, 1863 |
| AvaI | CYCGRG | 2 cut(s) | 1861, 2388 |
| AvaII | GGWCC | 2 cut(s) | 2261, 2318 |
| BaeGI | GKGCMC | 1 cut(s) | 1882 |
| BalI | TGGCCA | 1 cut(s) | 1958 |
| BamHI | GGATCC | 2 cut(s) | 1652, 2185 |
| BanI | GGYRCC | 1 cut(s) | 1879 |
| BanII | GRGCYC | 1 cut(s) | 1539 |
| BbsI | GAAGAC | 2 cut(s) | 1002, 1903 |
| Bbv12I | GWGCWC | 1 cut(s) | 2481 |
| BbvI | GCAGC | 4 cut(s) | 1090, 1274, 1331, 2449 |
| BccI | CCATC | 9 cut(s) | 152, 222, 899, 997, 1046, 1526, 1622, 2169, 2282 |
| BceAI | ACGGC | 1 cut(s) | 2623 |
| BciT130I | CCWGG | 5 cut(s) | 1173, 1667, 2183, 2235, 2426 |
| BclI | TGATCA | 1 cut(s) | 1438 |
| BcnI | CCSGG | 3 cut(s) | 1731, 1862, 1863 |
| BcoDI | GTCTC | 2 cut(s) | 1247, 1404 |
| BfaI | CTAG | 5 cut(s) | 116, 567, 1280, 2214, 2459 |
| BfuAI | ACCTGC | 3 cut(s) | 936, 1216, 1576 |
| BglII | AGATCT | 2 cut(s) | 890, 2072 |
| BisI | GCNGC | 6 cut(s) | 797, 1079, 1288, 1345, 2463, 2507 |
| BlsI | GCNGC | 6 cut(s) | 798, 1080, 1289, 1346, 2464, 2508 |
| BmcAI | AGTACT | 1 cut(s) | 1996 |
| Bme1390I | CCNGG | 8 cut(s) | 1173, 1667, 1731, 1862, 1863, 2183, 2235, 2426 |
| Bme18I | GGWCC | 2 cut(s) | 2261, 2318 |
| BmeT110I | CYCGRG | 2 cut(s) | 1861, 2388 |
| BmgBI | CACGTC | 2 cut(s) | 1414, 2605 |
| BmgT120I | GGNCC | 2 cut(s) | 2261, 2318 |
| BmiI | GGNNCC | 6 cut(s) | 1098, 1654, 1881, 2187, 2263, 2319 |
| BmrFI | CCNGG | 8 cut(s) | 1173, 1667, 1731, 1862, 1863, 2183, 2235, 2426 |
| BmsI | GCATC | 2 cut(s) | 176, 1323 |
| BpiI | GAAGAC | 2 cut(s) | 1002, 1903 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 2638 |
| BpmI | CTGGAG | 2 cut(s) | 518, 2495 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1431, 2022 |
| BpuEI | CTTGAG | 2 cut(s) | 1642, 1785 |
| BpuMI | CCSGG | 3 cut(s) | 1731, 1862, 1863 |
| BsaAI | YACGTR | 1 cut(s) | 2374 |
| BsaBI | GATNNNNATC | 1 cut(s) | 346 |
| BsaJI | CCNNGG | 6 cut(s) | 44, 1665, 1826, 1860, 1861, 2639 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 33, 1805, 1835 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1827, 1868, 2219, 2394, 2395 |
| Bse1I | ACTGG | 2 cut(s) | 1959, 2478 |
| Bse3DI | GCAATG | 2 cut(s) | 549, 2224 |
| Bse8I | GATNNNNATC | 1 cut(s) | 346 |
| BseBI | CCWGG | 5 cut(s) | 1173, 1667, 2183, 2235, 2426 |
| BseDI | CCNNGG | 6 cut(s) | 44, 1665, 1826, 1860, 1861, 2639 |
| BseGI | GGATG | 4 cut(s) | 88, 163, 496, 989 |
| BseJI | GATNNNNATC | 1 cut(s) | 346 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1827, 1868, 2219, 2394, 2395 |
| BseMI | GCAATG | 2 cut(s) | 549, 2224 |
| BseMII | CTCAG | 6 cut(s) | 114, 1035, 1239, 1422, 1460, 2036 |
| BseNI | ACTGG | 2 cut(s) | 1959, 2478 |
| BseSI | GKGCMC | 1 cut(s) | 1882 |
| BseXI | GCAGC | 4 cut(s) | 1090, 1274, 1331, 2449 |
| BsgI | GTGCAG | 2 cut(s) | 1273, 2147 |
| BshFI | GGCC | 4 cut(s) | 961, 1958, 2233, 2406 |
| BshNI | GGYRCC | 1 cut(s) | 1879 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2481 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1861, 2388 |
| BsiSI | CCGG | 2 cut(s) | 1730, 1862 |
| BslFI | GGGAC | 6 cut(s) | 562, 1412, 1773, 2247, 2284, 2331 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1827, 1868, 2219, 2394, 2395 |
| BsmAI | GTCTC | 2 cut(s) | 1247, 1404 |
| BsmBI | CGTCTC | 1 cut(s) | 1404 |
| BsmFI | GGGAC | 6 cut(s) | 562, 1412, 1773, 2247, 2284, 2331 |
| BsmI | GAATGC | 1 cut(s) | 169 |
| BsnI | GGCC | 4 cut(s) | 961, 1958, 2233, 2406 |
| BsoBI | CYCGRG | 2 cut(s) | 1861, 2388 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1539, 1882, 2481 |
| Bsp1407I | TGTACA | 1 cut(s) | 1505 |
| BspACI | CCGC | 6 cut(s) | 796, 1086, 1597, 2507, 2543, 2636 |
| BspANI | GGCC | 4 cut(s) | 961, 1958, 2233, 2406 |
| BspCNI | CTCAG | 6 cut(s) | 115, 1036, 1240, 1423, 1461, 2035 |
| BspHI | TCATGA | 1 cut(s) | 1516 |
| BspLI | GGNNCC | 6 cut(s) | 1098, 1654, 1881, 2187, 2263, 2319 |
| BspMI | ACCTGC | 3 cut(s) | 936, 1216, 1576 |
| BspPI | GGATC | 6 cut(s) | 1024, 1647, 1660, 1873, 2180, 2193 |
| BspT107I | GGYRCC | 1 cut(s) | 1879 |
| BsrDI | GCAATG | 2 cut(s) | 549, 2224 |
| BsrGI | TGTACA | 1 cut(s) | 1505 |
| BsrI | ACTGG | 2 cut(s) | 1959, 2478 |
| BssECI | CCNNGG | 6 cut(s) | 44, 1665, 1826, 1860, 1861, 2639 |
| BssT1I | CCWWGG | 1 cut(s) | 1826 |
| Bst2UI | CCWGG | 5 cut(s) | 1173, 1667, 2183, 2235, 2426 |
| Bst6I | CTCTTC | 9 cut(s) | 28, 168, 204, 210, 303, 525, 531, 1044, 1258 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2441 |
| BstAUI | TGTACA | 1 cut(s) | 1505 |
| BstBAI | YACGTR | 1 cut(s) | 2374 |
| BstC8I | GCNNGC | 2 cut(s) | 794, 1358 |
| BstDEI | CTNAG | 6 cut(s) | 123, 1044, 1248, 1431, 1469, 2022 |
| BstDSI | CCRYGG | 2 cut(s) | 44, 2639 |
| BstF5I | GGATG | 4 cut(s) | 88, 163, 496, 989 |
| BstMAI | GTCTC | 2 cut(s) | 1247, 1404 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 1165, 1353, 2018, 2441 |
| BstNI | CCWGG | 5 cut(s) | 1173, 1667, 2183, 2235, 2426 |
| BstNSI | RCATGY | 1 cut(s) | 2469 |
| BstSCI | CCNGG | 8 cut(s) | 1171, 1665, 1729, 1860, 1861, 2181, 2233, 2424 |
| BstSLI | GKGCMC | 1 cut(s) | 1882 |
| BstV1I | GCAGC | 4 cut(s) | 1090, 1274, 1331, 2449 |
| BstV2I | GAAGAC | 2 cut(s) | 1002, 1903 |
| BstX2I | RGATCY | 5 cut(s) | 890, 1652, 1865, 2072, 2185 |
| BstYI | RGATCY | 5 cut(s) | 890, 1652, 1865, 2072, 2185 |
| BsuRI | GGCC | 4 cut(s) | 961, 1958, 2233, 2406 |
| BtgI | CCRYGG | 2 cut(s) | 44, 2639 |
| BtrI | CACGTC | 2 cut(s) | 1414, 2605 |
| BtsCI | GGATG | 4 cut(s) | 88, 163, 496, 989 |
| BtsI | GCAGTG | 1 cut(s) | 2494 |
| BtsIMutI | CAGTG | 2 cut(s) | 1779, 2494 |
| BveI | ACCTGC | 3 cut(s) | 936, 1216, 1576 |
| Cac8I | GCNNGC | 2 cut(s) | 794, 1358 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1400, 1430 |
| CciI | TCATGA | 1 cut(s) | 1516 |
| Cfr13I | GGNCC | 2 cut(s) | 2261, 2318 |
| Cfr9I | CCCGGG | 1 cut(s) | 1861 |
| Csp6I | GTAC | 5 cut(s) | 870, 1506, 1604, 1621, 1995 |
| CviAII | CATG | 7 cut(s) | 1075, 1318, 1517, 1540, 1835, 2466, 2620 |
| CviQI | GTAC | 5 cut(s) | 870, 1506, 1604, 1621, 1995 |
| DdeI | CTNAG | 6 cut(s) | 123, 1044, 1248, 1431, 1469, 2022 |
| DraI | TTTAAA | 1 cut(s) | 1369 |
| DraIII | CACNNNGTG | 1 cut(s) | 1733 |
| EaeI | YGGCCR | 1 cut(s) | 1956 |
| Eam1104I | CTCTTC | 9 cut(s) | 28, 168, 204, 210, 303, 525, 531, 1044, 1258 |
| EarI | CTCTTC | 9 cut(s) | 28, 168, 204, 210, 303, 525, 531, 1044, 1258 |
| Eco130I | CCWWGG | 1 cut(s) | 1826 |
| Eco24I | GRGCYC | 1 cut(s) | 1539 |
| Eco32I | GATATC | 1 cut(s) | 2140 |
| Eco47I | GGWCC | 2 cut(s) | 2261, 2318 |
| Eco57I | CTGAAG | 1 cut(s) | 2257 |
| Eco88I | CYCGRG | 2 cut(s) | 1861, 2388 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2261 |
| EcoRI | GAATTC | 1 cut(s) | 1975 |
| EcoRII | CCWGG | 5 cut(s) | 1171, 1665, 2181, 2233, 2424 |
| EcoRV | GATATC | 1 cut(s) | 2140 |
| EcoT14I | CCWWGG | 1 cut(s) | 1826 |
| EcoT22I | ATGCAT | 1 cut(s) | 169 |
| EcoT38I | GRGCYC | 1 cut(s) | 1539 |
| ErhI | CCWWGG | 1 cut(s) | 1826 |
| Esp3I | CGTCTC | 1 cut(s) | 1404 |
| FaeI | CATG | 7 cut(s) | 1078, 1321, 1520, 1543, 1838, 2469, 2623 |
| FaqI | GGGAC | 6 cut(s) | 562, 1412, 1773, 2247, 2284, 2331 |
| FatI | CATG | 7 cut(s) | 1074, 1317, 1516, 1539, 1834, 2465, 2619 |
| FauI | CCCGC | 2 cut(s) | 1590, 2536 |
| FauNDI | CATATG | 1 cut(s) | 646 |
| FbaI | TGATCA | 1 cut(s) | 1438 |
| FblI | GTMKAC | 1 cut(s) | 1636 |
| Fnu4HI | GCNGC | 6 cut(s) | 797, 1079, 1288, 1345, 2463, 2507 |
| FokI | GGATG | 4 cut(s) | 95, 170, 503, 976 |
| FriOI | GRGCYC | 1 cut(s) | 1539 |
| Fsp4HI | GCNGC | 6 cut(s) | 797, 1079, 1288, 1345, 2463, 2507 |
| FspBI | CTAG | 5 cut(s) | 116, 567, 1280, 2214, 2459 |
| GluI | GCNGC | 6 cut(s) | 797, 1079, 1288, 1345, 2463, 2507 |
| GsuI | CTGGAG | 2 cut(s) | 518, 2495 |
| HaeIII | GGCC | 4 cut(s) | 961, 1958, 2233, 2406 |
| HapII | CCGG | 2 cut(s) | 1730, 1862 |
| Hin1II | CATG | 7 cut(s) | 1078, 1321, 1520, 1543, 1838, 2469, 2623 |
| HincII | GTYRAC | 1 cut(s) | 2151 |
| HindII | GTYRAC | 1 cut(s) | 2151 |
| HindIII | AAGCTT | 1 cut(s) | 462 |
| HinfI | GANTC | 8 cut(s) | 36, 49, 77, 752, 865, 995, 1337, 1562 |
| HpaII | CCGG | 2 cut(s) | 1730, 1862 |
| Hpy166II | GTNNAC | 6 cut(s) | 286, 478, 1382, 1637, 2151, 2471 |
| Hpy8I | GTNNAC | 6 cut(s) | 286, 478, 1382, 1637, 2151, 2471 |
| Hpy99I | CGWCG | 2 cut(s) | 695, 698 |
| HpyAV | CCTTC | 9 cut(s) | 553, 591, 630, 1201, 1514, 1604, 1742, 2163, 2520 |
| HpyCH4IV | ACGT | 4 cut(s) | 1413, 1900, 2373, 2604 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 1165, 1353, 2018, 2441 |
| HpyF3I | CTNAG | 6 cut(s) | 123, 1044, 1248, 1431, 1469, 2022 |
| HpySE526I | ACGT | 4 cut(s) | 1413, 1900, 2373, 2604 |
| Hsp92II | CATG | 7 cut(s) | 1078, 1321, 1520, 1543, 1838, 2469, 2623 |
| Ksp22I | TGATCA | 1 cut(s) | 1438 |
| LmnI | GCTCC | 3 cut(s) | 152, 991, 2476 |
| Lsp1109I | GCAGC | 4 cut(s) | 1090, 1274, 1331, 2449 |
| LweI | GCATC | 2 cut(s) | 176, 1323 |
| MaeI | CTAG | 5 cut(s) | 116, 567, 1280, 2214, 2459 |
| MaeII | ACGT | 4 cut(s) | 1413, 1900, 2373, 2604 |
| MaeIII | GTNAC | 2 cut(s) | 923, 2514 |
| MfeI | CAATTG | 1 cut(s) | 1925 |
| MflI | RGATCY | 5 cut(s) | 890, 1652, 1865, 2072, 2185 |
| MhlI | GDGCHC | 3 cut(s) | 1539, 1882, 2481 |
| MlsI | TGGCCA | 1 cut(s) | 1958 |
| MluNI | TGGCCA | 1 cut(s) | 1958 |
| MlyI | GAGTC | 1 cut(s) | 45 |
| MmeI | TCCRAC | 4 cut(s) | 111, 135, 1819, 2213 |
| Mox20I | TGGCCA | 1 cut(s) | 1958 |
| Mph1103I | ATGCAT | 1 cut(s) | 169 |
| MscI | TGGCCA | 1 cut(s) | 1958 |
| MseI | TTAA | 8 cut(s) | 615, 1221, 1368, 1682, 1767, 1904, 2006, 2537 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1521 |
| Msp20I | TGGCCA | 1 cut(s) | 1958 |
| MspA1I | CMGCKG | 1 cut(s) | 1081 |
| MspI | CCGG | 2 cut(s) | 1730, 1862 |
| MspR9I | CCNGG | 8 cut(s) | 1173, 1667, 1731, 1862, 1863, 2183, 2235, 2426 |
| MunI | CAATTG | 1 cut(s) | 1925 |
| Mva1269I | GAATGC | 1 cut(s) | 169 |
| MvaI | CCWGG | 5 cut(s) | 1173, 1667, 2183, 2235, 2426 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 1165, 1353, 2018, 2441 |
| NciI | CCSGG | 3 cut(s) | 1731, 1862, 1863 |
| NdeI | CATATG | 1 cut(s) | 646 |
| NlaIII | CATG | 7 cut(s) | 1078, 1321, 1520, 1543, 1838, 2469, 2623 |
| NlaIV | GGNNCC | 6 cut(s) | 1098, 1654, 1881, 2187, 2263, 2319 |
| NmuCI | GTSAC | 1 cut(s) | 2514 |
| NsiI | ATGCAT | 1 cut(s) | 169 |
| NspI | RCATGY | 1 cut(s) | 2469 |
| PagI | TCATGA | 1 cut(s) | 1516 |
| PaqCI | CACCTGC | 1 cut(s) | 1576 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 699, 2601 |
| PctI | GAATGC | 1 cut(s) | 169 |
| PfeI | GAWTC | 7 cut(s) | 49, 77, 752, 865, 995, 1337, 1562 |
| PfoI | TCCNGGA | 2 cut(s) | 2181, 2424 |
| PkrI | GCNGC | 6 cut(s) | 798, 1080, 1289, 1346, 2464, 2508 |
| PleI | GAGTC | 1 cut(s) | 44 |
| PpsI | GAGTC | 1 cut(s) | 44 |
| Ppu21I | YACGTR | 1 cut(s) | 2374 |
| PpuMI | RGGWCCY | 1 cut(s) | 2261 |
| PshBI | ATTAAT | 1 cut(s) | 2537 |
| PsiI | TTATAA | 1 cut(s) | 63 |
| Psp5II | RGGWCCY | 1 cut(s) | 2261 |
| Psp6I | CCWGG | 5 cut(s) | 1171, 1665, 2181, 2233, 2424 |
| PspGI | CCWGG | 5 cut(s) | 1171, 1665, 2181, 2233, 2424 |
| PspN4I | GGNNCC | 6 cut(s) | 1098, 1654, 1881, 2187, 2263, 2319 |
| PspPI | GGNCC | 2 cut(s) | 2261, 2318 |
| PspPPI | RGGWCCY | 1 cut(s) | 2261 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1400, 1430 |
| PsuI | RGATCY | 5 cut(s) | 890, 1652, 1865, 2072, 2185 |
| PvuII | CAGCTG | 1 cut(s) | 1081 |
| RsaI | GTAC | 5 cut(s) | 871, 1507, 1605, 1622, 1996 |
| RsaNI | GTAC | 5 cut(s) | 870, 1506, 1604, 1621, 1995 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1521 |
| SaqAI | TTAA | 8 cut(s) | 615, 1221, 1368, 1682, 1767, 1904, 2006, 2537 |
| SatI | GCNGC | 6 cut(s) | 797, 1079, 1288, 1345, 2463, 2507 |
| Sau96I | GGNCC | 2 cut(s) | 2261, 2318 |
| ScaI | AGTACT | 1 cut(s) | 1996 |
| SchI | GAGTC | 1 cut(s) | 45 |
| ScrFI | CCNGG | 8 cut(s) | 1173, 1667, 1731, 1862, 1863, 2183, 2235, 2426 |
| SduI | GDGCHC | 3 cut(s) | 1539, 1882, 2481 |
| SfaNI | GCATC | 2 cut(s) | 176, 1323 |
| SinI | GGWCC | 2 cut(s) | 2261, 2318 |
| SmaI | CCCGGG | 1 cut(s) | 1863 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1521 |
| SmlI | CTYRAG | 2 cut(s) | 1657, 1800 |
| SmoI | CTYRAG | 2 cut(s) | 1657, 1800 |
| SsiI | CCGC | 6 cut(s) | 796, 1086, 1597, 2507, 2543, 2636 |
| SspMI | CTAG | 5 cut(s) | 116, 567, 1280, 2214, 2459 |
| StyD4I | CCNGG | 8 cut(s) | 1171, 1665, 1729, 1860, 1861, 2181, 2233, 2424 |
| StyI | CCWWGG | 1 cut(s) | 1826 |
| TaiI | ACGT | 4 cut(s) | 1416, 1903, 2376, 2607 |
| TaqI | TCGA | 2 cut(s) | 350, 1212 |
| TaqII | GACCGA | 1 cut(s) | 42 |
| TatI | WGTACW | 2 cut(s) | 1505, 1994 |
| TauI | GCSGC | 2 cut(s) | 799, 2509 |
| TfiI | GAWTC | 7 cut(s) | 49, 77, 752, 865, 995, 1337, 1562 |
| Tru1I | TTAA | 8 cut(s) | 615, 1221, 1368, 1682, 1767, 1904, 2006, 2537 |
| Tru9I | TTAA | 8 cut(s) | 615, 1221, 1368, 1682, 1767, 1904, 2006, 2537 |
| TscAI | CASTG | 2 cut(s) | 1779, 2494 |
| TseFI | GTSAC | 1 cut(s) | 2514 |
| TseI | GCWGC | 4 cut(s) | 1078, 1287, 1344, 2462 |
| Tsp45I | GTSAC | 1 cut(s) | 2514 |
| TspGWI | ACGGA | 2 cut(s) | 61, 2076 |
| TspMI | CCCGGG | 1 cut(s) | 1861 |
| TspRI | CASTG | 2 cut(s) | 1779, 2494 |
| VpaK11BI | GGWCC | 2 cut(s) | 2261, 2318 |
| VspI | ATTAAT | 1 cut(s) | 2537 |
| XapI | RAATTY | 4 cut(s) | 710, 1131, 1236, 1975 |
| XceI | RCATGY | 1 cut(s) | 2469 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2311 |
| XmaI | CCCGGG | 1 cut(s) | 1861 |
| XmiI | GTMKAC | 1 cut(s) | 1636 |
| XspI | CTAG | 5 cut(s) | 116, 567, 1280, 2214, 2459 |
| ZrmI | AGTACT | 1 cut(s) | 1996 |
| Zsp2I | ATGCAT | 1 cut(s) | 169 |