RchiOBHm_Chr3g0455401

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000031
Physical Location & Seq
Reverse (-)
5133622 .. 5137963
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42233

Sequence Viewer

Length: 3423 bp
ATGGTAATCGGGGACTTCAACGCTATGTTAGGAGCCCATGAAAAATCAGGAGGCCCATCTCCCTCTCGCCTCTCCTGTGCAGATTTTCAAAGTATGTCAGATACTTGTGATTTTGTTCATCTAGATACTTCCGGTTTGTTCTTTACTTGGTCAAATGGTTGGAGCACCCGTGGACATGTTGAGCTTCGTTTGGACAGATGTCTTTGTGATACCACTTGGTTTGAAGCTTGGCCTTTTACTAGTTGTGTTGCTCTTCCCCGCGTTGCTTCTGATCATTCTCCATTGATTTTCTCTGCTTCAAAACTCTCCCCAAGTGGTCCCAAGCCTTTCAAGTTTCAGTCTATGTGGTTGCAGCATCCAAATTTTCATGCAGTAGTGTCTAATTGTTGGAATTCCTTTCAACTTTGTGGCTGCCCAATGTTTGTTACTTTGCAGAAATTAAAGGCTTTGAAACATTGTTTGCGTGATTGGAACAAAGTGGTTTTCGGTAATGTCCATCAAAAGGTTGCTACTGCTCGTGAAAATTTAGTCAACATTCAACAAGATATCGCGACCCGTGGCATGACTGATCAAAGGTTTGAGGATGAGATTGCTGCAAAGTCTGCAGTCTTAGATGCTCTGAGGATGCAAGAAACTTATTGGAAAGATAGAGCTCGGGTTAAGTGGTTTACTGATGGGGATCGCAGTACATCTTTTTTCCATACTTATGCCAAGGTTCGTGCAGCCAAAAGTCAAATGTCTTCTATCCGTGTGGGGGAGAGGATACTTACTGACCCTTCAGACATTGCAGCACATACTGTGGCTTTTTATCAGAATCTCTATGATATTTCCTCCCCTTCTACAAATGTGGATGAGGTTTGTTCAGTTATTCCATCTCTTGTGACTGACGATGAAAATGTGGTGCTATCTGCAATTCCTACATCAGAGGAAATTAGAAGTGCAGTCTTTTCTATGGATGGTTCTAGTGCTCCAGGGCCTGATGGTTTTTCAGGGTCTTTTTATCAAGCTTGTTGGGATATTGTTGGTTCTGATGTGGTGGCATGTGTTCGTCAATTTTTTCTTCAAAACTGGATCCTCCCCAACATGAATTGCAATTTTTTAGTTCTCATTCCAAAAGTCCCAAATGCTCAGCTTATTACTCAGTTTCGCCCAATTGCCTTAGCCAATTTTCTCTTCAAGATTATACCTAAGATTTTGGCTTCTCGCTTGGGGTCTATTGCTACACGTATAATTTCTCCTCAGCAATCAGCTTTTCTGCCTGGTAGGCGTATTAATCATTGTATTGGTATGGTTTCGGAATGCTTTAACTTATTGGACCGAAAGGCTTATGGTGGAAATGTGGGAATCAAAGTGGATATAGCAAAGGCGTTTGACACTCTTAACTGGGATTTTCTGCTCCGAGTTTTAAGTAATTTTGGGTTCTCTCCCACTTTCACTAGTTGGATCAGCTCTATTCTTCACTCAGCAAGACTATCTCTTTTAATCAATGGCTCTCCTCAAGGTTTCTTTACGTGTTCTCGTGGTGTTAGACAAGGAGATCCCCTTTCTCCACTTTTATTCTGTCTTGCTGAGGAGGCTCTTAGCCGGGGCCTTCACTTACTTCTCCAAACCCGTCAATTGAAGCCAATATCTTTTCCCAGGAAATGCACTGCACCGTCTCATGTGTTATATGCAGACGACCTCATGGTGTTTTGTCGTGGGGACAAAAACTCTCTTAAACGGCTGCGTGGGTTTTTTGACCGTTATAGTGCAGCTTCAGGGCAATTCATTAATGCGGATAAAAGCACTTTCTATCTTGGAGCTAGGTCTCGTCACAGGAAAGGTACAGTTCAACGTATATTGGGTTTTCATGCAGGGCGCCTACCTTTTATCTATCTGGGAGTTCCTATTTTTTGTGGAAAACCCCGCAGCTGCCATCTCCAAGTTATTGCCGATAGAGCTAAGTCAAGGTTAATGGGTTGGCAGGGGAGGCTTCTTTCTATGGCTGGACGCACTCAACTTGTGCAATCTGTTTTTCAAAGTATGCTTCTCCATAGTTTTTCTGTGTATCAATGGCCTGCATACCTTGTGAAGAAGTTATCTACATGGGCGCGTAATTTTATATGGTCTGGAAATATCGAGACTCGGAAAATTGTCACTGTTACTTGGACTCAAGTTTGTGCTCCGAAAAAAGAGGGCGGGGTGGGTATTCGTGACCTGGCAACTCTTAATTCGGCAGCTGTTTTAAGGTTTGCATGGAACTCTTTCACTTCTTTGACCCAATGGGGTGATTATATGCGAACTCGTTATCCTTTATTATCTAGTTCCAAAGTGAGCTACCGCAAATCCTCAATCTGGCCAGGTTTCCGCTCTATTGCTCTTCATATCACCCACAATTGTCGTTGGATCATTGGAGATGGTCGTTCAGTGTCATTTTGGAAAGATAAATGGCTTCAAGATCCAATTTTGGAAATGTTGGGTTTTTTCGACTGGTCAGGATTCAGTGATCTTCGTGTGGCTGATTTCATCTCTGATCATTCTTGGCAGTTTCCCTCTTTTTTCCTAGATACCTTTCCAGACTTGTACAGGAAAATTTCTGATATCTGTCTCCCTTTAGACACTGAACCTGATATGCTCATTTGGGAGTCTACAGCCTCTGGAGAGTTATCCTTCGCTGATTCTTACAACTTGCTTAGACGACACTTTATTTTGAGAGACTGGGCTTCTACTATTTGGCATTCCTTCATTCCTCCGCGTTTTTCTTTCTTGGCTTGGAGGATTCTCTTAGATCGCTTGCCCACTGATGATCGGCTGAAAAGAGGTGGGATTCCGATTGTGTCCATCTGCCAATTATGCAGCAGTTCGGCAGAGTCAGCTTTACACTTATTTTTACACTGCCCTTTCTCCCAACATCTTTGGGGATGGCTGGCAACTCAGTTTGGAACCTCATTTCCTGCTTATGACTCTCTGTTGGATTTTTGGGTTGGCTTTTGTCACAAGGGTTTTTCCCCTCAGCTTTATAATTTGTGGTTGGCAGCTGGTTTGCTTACTTTCATGGAAATTTGGAAGGCGCGAAATAGACTCAGGTTTGATAATCGCTCCCCTATTTTTTCTACACTGTGTTGTTCAATTATGGCATGGATTCGGCAATTCGGTTCTCTTGTTCCTGGTTACTATAAAGGCGTCCTGGATTCCCGCCTTCTCTCTTCCCTTGGGGTGTGCCCCAAGCCTCGTAAAGCGCCCAAGATTCAACGTGTCCTATGGCATCCCCCCCTCCCTCCTTGGGTTAAAGTTAACACTGATGGTCTGGCGAAAGGTAATCCTGGTCCTGCAGCTTGTGGAGGAGTTTTTCGCGATGCTTCTGGGGTTTATTTAGGGAGTTTTTGCCAACCTCTGGAGGTTGGACTACTCTGTGGTTGGAAAGTGATTCTATTAGTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

1140

Amino Acids

128.67

Weight (kDa)

9.31

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 373 - 629 7.4e-31 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 885 - 966 4.1e-23 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05755
fragaria_vesca FvH4_1g13571 FvH4_2g06951 FvH4_2g09612 FvH4_3g24991 FvH4_3g39131 FvH4_4g01331 FvH4_5g11071 FvH4_5g22144 FvH4_5g22144 FvH4_5g27541 FvH4_5g38601 FvH4_6g09211 FvH4_6g17161 FvH4_6g30101 FvH4_6g30102 FvH4_6g42420 FvH4_6g42420 FvH4_6g42420 FvH4_6g42420 FvH4_6g42420 FvH4_6g42420 FvH4_6g51131 FvH4_7g28851
malus_domestica MD02G1021900.v1.1 MD02G1050700.v1.1 MD02G1314000.v1.1 MD05G1244100.v1.1 MD07G1021300.v1.1 MD09G1113200.v1.1 MD09G1113300.v1.1 MD13G1019000.v1.1 MD17G1103400.v1.1
prunus_persica Prupe.1G484600_v2.0.a1 Prupe.3G211200_v2.0.a1 Prupe.3G211200_v2.0.a1 Prupe.4G283800_v2.0.a1
pyrus_communis pycom01g13080 pycom03g05530 pycom05g09360 pycom05g20240 pycom06g06350 pycom06g14660 pycom09g03750 pycom13g22930 pycom14g00800 pycom15g35930 pycom17g09770 pycom17g25010
rosa_chinensis RchiOBHm_Chr1g0316991 RchiOBHm_Chr1g0321411 RchiOBHm_Chr1g0341061 RchiOBHm_Chr1g0352021 RchiOBHm_Chr1g0357231 RchiOBHm_Chr1g0361641 RchiOBHm_Chr1g0379151 RchiOBHm_Chr2g0084861 RchiOBHm_Chr2g0092021 RchiOBHm_Chr2g0102721 RchiOBHm_Chr2g0119161 RchiOBHm_Chr2g0120021 RchiOBHm_Chr2g0120031 RchiOBHm_Chr2g0120451 RchiOBHm_Chr2g0129631 RchiOBHm_Chr2g0133661 RchiOBHm_Chr2g0140031 RchiOBHm_Chr2g0154361 RchiOBHm_Chr2g0158311 RchiOBHm_Chr2g0160431 RchiOBHm_Chr2g0167951 RchiOBHm_Chr2g0168801 RchiOBHm_Chr3g0451531 RchiOBHm_Chr3g0453641 RchiOBHm_Chr3g0453831 RchiOBHm_Chr3g0455401 RchiOBHm_Chr3g0457461 RchiOBHm_Chr3g0459251 RchiOBHm_Chr3g0462451 RchiOBHm_Chr3g0465511 RchiOBHm_Chr3g0466111 RchiOBHm_Chr3g0469201 RchiOBHm_Chr3g0472791 RchiOBHm_Chr3g0472841 RchiOBHm_Chr4g0391691 RchiOBHm_Chr4g0393351 RchiOBHm_Chr4g0395061 RchiOBHm_Chr4g0395191 RchiOBHm_Chr4g0397541 RchiOBHm_Chr4g0402261 RchiOBHm_Chr4g0409421 RchiOBHm_Chr4g0417481 RchiOBHm_Chr4g0428291 RchiOBHm_Chr4g0434281 RchiOBHm_Chr4g0437231 RchiOBHm_Chr4g0439261 RchiOBHm_Chr5g0000531 RchiOBHm_Chr5g0016091 RchiOBHm_Chr5g0018801 RchiOBHm_Chr5g0026401 RchiOBHm_Chr5g0051821 RchiOBHm_Chr5g0062261 RchiOBHm_Chr5g0070711 RchiOBHm_Chr5g0074731 RchiOBHm_Chr5g0077831 RchiOBHm_Chr6g0251621 RchiOBHm_Chr6g0253181 RchiOBHm_Chr6g0258241 RchiOBHm_Chr6g0259301 RchiOBHm_Chr6g0263381 RchiOBHm_Chr6g0264461 RchiOBHm_Chr6g0278601 RchiOBHm_Chr6g0299951 RchiOBHm_Chr7g0184971 RchiOBHm_Chr7g0185631 RchiOBHm_Chr7g0200601 RchiOBHm_Chr7g0206171 RchiOBHm_Chr7g0222341 RchiOBHm_Chr7g0237631
rosa_laevigata RLG00000012173 RLG00000021075
rosa_multiflora Rmu_co8065174.1_g000001 Rmu_co8074360.1_g000001 Rmu_co8095164.1_g000001 Rmu_co8204978.1_g000001 Rmu_co8329721.1_g000001 Rmu_co8347171.1_g000001 Rmu_co8395661.1_g000001 Rmu_co8466967.1_g000001 Rmu_sc0000030.1_g000008 Rmu_sc0000290.1_g000015 Rmu_sc0000294.1_g000012 Rmu_sc0000319.1_g000020 Rmu_sc0000418.1_g000014 Rmu_sc0000423.1_g000010 Rmu_sc0000427.1_g000013 Rmu_sc0000430.1_g000011 Rmu_sc0000487.1_g000037 Rmu_sc0000765.1_g000028 Rmu_sc0000770.1_g000037 Rmu_sc0000785.1_g000011 Rmu_sc0000811.1_g000011 Rmu_sc0000848.1_g000004 Rmu_sc0000897.1_g000007 Rmu_sc0000955.1_g000014 Rmu_sc0001004.1_g000019 Rmu_sc0001010.1_g000068 Rmu_sc0001021.1_g000008 Rmu_sc0001084.1_g000013 Rmu_sc0001111.1_g000002 Rmu_sc0001190.1_g000015 Rmu_sc0001308.1_g000013 Rmu_sc0001398.1_g000010 Rmu_sc0001680.1_g000005 Rmu_sc0001926.1_g000017 Rmu_sc0001942.1_g000074 Rmu_sc0002068.1_g000010 Rmu_sc0002113.1_g000019 Rmu_sc0002299.1_g000032 Rmu_sc0002531.1_g000026 Rmu_sc0002549.1_g000005 Rmu_sc0002634.1_g000001 Rmu_sc0002640.1_g000014 Rmu_sc0002831.1_g000005 Rmu_sc0002848.1_g000018 Rmu_sc0003005.1_g000027 Rmu_sc0003076.1_g000012 Rmu_sc0003124.1_g000001 Rmu_sc0003143.1_g000004 Rmu_sc0003143.1_g000005 Rmu_sc0003143.1_g000006 Rmu_sc0003302.1_g000009 Rmu_sc0003368.1_g000035 Rmu_sc0003462.1_g000001 Rmu_sc0003692.1_g000016 Rmu_sc0003855.1_g000007 Rmu_sc0003858.1_g000008 Rmu_sc0004277.1_g000031 Rmu_sc0004732.1_g000013 Rmu_sc0004748.1_g000005 Rmu_sc0005091.1_g000024 Rmu_sc0005124.1_g000001 Rmu_sc0005167.1_g000001 Rmu_sc0005242.1_g000003 Rmu_sc0005486.1_g000005 Rmu_sc0005597.1_g000008 Rmu_sc0005653.1_g000004 Rmu_sc0006080.1_g000016 Rmu_sc0006371.1_g000003 Rmu_sc0006415.1_g000001 Rmu_sc0006522.1_g000003 Rmu_sc0006596.1_g000007 Rmu_sc0006991.1_g000005 Rmu_sc0007070.1_g000004 Rmu_sc0007737.1_g000005 Rmu_sc0007856.1_g000001 Rmu_sc0008241.1_g000001 Rmu_sc0008393.1_g000006 Rmu_sc0008563.1_g000008 Rmu_sc0008904.1_g000006 Rmu_sc0010313.1_g000014 Rmu_sc0010693.1_g000002 Rmu_sc0010917.1_g000011 Rmu_sc0011232.1_g000003 Rmu_sc0011978.1_g000005 Rmu_sc0012782.1_g000003 Rmu_sc0013716.1_g000002 Rmu_sc0015429.1_g000002 Rmu_sc0015649.1_g000014 Rmu_sc0021955.1_g000004 Rmu_sc0024117.1_g000001 Rmu_sc0024816.1_g000001 Rmu_sc0024816.1_g000006 Rmu_sc0026593.1_g000003 Rmu_sc0028245.1_g000001 Rmu_sc0029783.1_g000006 Rmu_sc0030817.1_g000001 Rmu_sc0031096.1_g000001 Rmu_sc0034014.1_g000001 Rmu_sc0036569.1_g000001 Rmu_sc0039034.1_g000001 Rmu_ssc0000004.1_g000015 Rmu_ssc0000053.1_g000002 Rmu_ssc0000126.1_g000069 Rmu_ssc0000211.1_g000020 Rmu_ssc0000295.1_g000005 Rmu_ssc0000359.1_g000057 Rmu_ssc0000385.1_g000011 Rmu_ssc0000416.1_g000004 Rmu_ssc0000434.1_g000018
rosa_roxburghii Rroxscaffold_159G00432920 Rroxscaffold_2G00091500 Rroxscaffold_2G00123510 Rroxscaffold_2G00124730 Rroxscaffold_2G00126030 Rroxscaffold_2G00138160 Rroxscaffold_2G00153260 Rroxscaffold_3G00224470 Rroxscaffold_3G00244670 Rroxscaffold_3G00261920 Rroxscaffold_3G00264150 Rroxscaffold_3G00270420 Rroxscaffold_4G00285150 Rroxscaffold_4G00299830 Rroxscaffold_4G00311290 Rroxscaffold_4G00323970 Rroxscaffold_5G00374550 Rroxscaffold_6G00427060 Rroxscaffold_7G00169160 Rroxscaffold_7G00174970
rosa_rugosa Rorug01G0012500 Rorug01G0046200 Rorug01G0118800 Rorug01G0120200 Rorug01G0135400.1 Rorug01G0174200 Rorug01G0204600 Rorug01G0253500 Rorug01G0290500 Rorug01G0290800 Rorug01G0312100 Rorug01G0333700 Rorug01G0339600 Rorug01G0377800 Rorug01G0400400 Rorug01G0402200 Rorug01G0410700 Rorug01G0431400 Rorug02G0011900 Rorug02G0012500 Rorug02G0022300 Rorug02G0062400 Rorug02G0085300 Rorug02G0091800 Rorug02G0104500 Rorug02G0125400 Rorug02G0131000 Rorug02G0147300 Rorug02G0171000 Rorug02G0181500 Rorug02G0196900 Rorug02G0207200 Rorug02G0226600 Rorug02G0241300 Rorug02G0274300 Rorug02G0280600 Rorug02G0280800 Rorug02G0304600 Rorug02G0304700 Rorug02G0338800 Rorug02G0375800 Rorug02G0379300 Rorug02G0379400 Rorug02G0447600 Rorug02G0459900 Rorug02G0467200 Rorug02G0535400 Rorug03G0072300 Rorug03G0218900 Rorug03G0223700 Rorug03G0231500 Rorug03G0257200 Rorug03G0257300 Rorug03G0257400 Rorug03G0265700 Rorug03G0290200 Rorug03G0291300 Rorug03G0311000 Rorug03G0326900 Rorug03G0332700 Rorug03G0352000 Rorug03G0369700.1 Rorug04G0014000 Rorug04G0014500 Rorug04G0124100 Rorug04G0142100 Rorug04G0151400 Rorug04G0160700 Rorug04G0186400 Rorug04G0227600 Rorug04G0253800 Rorug04G0274400 Rorug04G0274600 Rorug04G0295600 Rorug04G0302000 Rorug04G0320600 Rorug04G0331300 Rorug04G0352200 Rorug04G0407000 Rorug04G0430300 Rorug04G0443000 Rorug04G0446400 Rorug04G0459000 Rorug05G0042400 Rorug05G0042500 Rorug05G0064900 Rorug05G0074300 Rorug05G0097700 Rorug05G0102300 Rorug05G0102300 Rorug05G0123700 Rorug05G0145800 Rorug05G0152900 Rorug05G0175900 Rorug05G0182100 Rorug05G0196000 Rorug05G0207000 Rorug05G0212800 Rorug05G0226400 Rorug05G0241500 Rorug05G0264800.1 Rorug05G0269600 Rorug05G0298200 Rorug05G0304300 Rorug05G0344300 Rorug05G0382300 Rorug05G0392300.1 Rorug05G0427900 Rorug05G0433500 Rorug05G0452700 Rorug05G0464600 Rorug05G0475800 Rorug05G0488600 Rorug05G0493000 Rorug05G0504800 Rorug05G0505500.1 Rorug05G0508400 Rorug05G0545800 Rorug05G0587000 Rorug05G0591100.1 Rorug06G0013200 Rorug06G0037700 Rorug06G0098200 Rorug06G0184500 Rorug06G0185300 Rorug06G0188200 Rorug06G0208300 Rorug06G0212900 Rorug06G0251500 Rorug06G0328000 Rorug06G0366100 Rorug06G0367400 Rorug06G0371200 Rorug06G0393500 Rorug06G0423000 Rorug06G0440500 Rorug06G0445400 Rorug06G0451700 Rorug06G0479400 Rorug06G0479500 Rorug06G0479600 Rorug06G0479700 Rorug06G0479800 Rorug06G0483100 Rorug07G0001200 Rorug07G0005200 Rorug07G0068600 Rorug07G0109300 Rorug07G0115500 Rorug07G0117000 Rorug07G0123500 Rorug07G0128000 Rorug07G0128100 Rorug07G0225500 Rorug07G0280200 Rorug07G0317300
rosa_samantha Rh1AG251400 Rh1BG133300 Rh1CG079900 Rh1CG235600 Rh1DG168000 Rh2AG533100 Rh2BG546900 Rh2CG517000 Rh2DG237900 Rh2DG555900 Rh3CG293400 Rh4AG419800 Rh4BG306300 Rh4CG446100 Rh5AG268900 Rh5AG282600 Rh5BG006600 Rh5BG345700 Rh5CG468100 Rh6CG190700 Rh6DG111900 Rh7BG172200 Rh7BG412200
rosa_wichuraiana Rw0G003620 Rw0G004000 Rw0G011770 Rw0G019070 Rw0G020070 Rw1G002990 Rw1G003550 Rw1G003860 Rw1G005930 Rw1G018430 Rw1G021160 Rw1G023820 Rw1G032700 Rw1G035790 Rw1G036160 Rw1G038660 Rw1G039380 Rw2G016120 Rw2G018680 Rw2G026860 Rw2G028670 Rw2G040870 Rw2G041040 Rw2G041410 Rw2G044140 Rw2G047570 Rw3G019760 Rw4G031020 Rw5G003700 Rw5G004830 Rw5G037470 Rw5G047400 Rw6G020150 Rw6G024010 Rw6G026600 Rw7G002480 Rw7G004430 Rw7G011870 Rw7G035520 Rw7G041260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3000
AccB1I GGYRCC 1 cut(s) 1857
AccB7I CCANNNNNTGG 1 cut(s) 3373
AccBSI CCGCTC 1 cut(s) 2349
AccI GTMKAC 1 cut(s) 2627
AccII CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
AciI CCGC 8 cut(s) 259, 1775, 1906, 2178, 2320, 2347, 2732, 3175
AclWI GGATC 7 cut(s) 687, 1066, 1079, 1451, 1532, 2393, 2432
AcoI YGGCCR 1 cut(s) 2336
AcsI RAATTY 5 cut(s) 361, 391, 523, 2571, 3039
AcuI CTGAAG 2 cut(s) 762, 1740
AcyI GRCGYC 2 cut(s) 1858, 3162
AfaI GTAC 3 cut(s) 688, 1825, 2564
AfiI CCNNNNNNNGG 5 cut(s) 502, 754, 2334, 3262, 3373
AflIII ACRYGT 4 cut(s) 175, 1223, 1511, 3232
AhlI ACTAGT 2 cut(s) 239, 1436
AjnI CCWGG 8 cut(s) 970, 1258, 1637, 2196, 2338, 3145, 3165, 3301
AjuI GAANNNNNNNTTGG 2 cut(s) 1157, 1189
AloI GAACNNNNNNTCC 4 cut(s) 2272, 2304, 2914, 2946
Alw21I GWGCWC 4 cut(s) 167, 655, 970, 2164
Alw26I GTCTC 5 cut(s) 1662, 1812, 2114, 2591, 2688
AlwI GGATC 7 cut(s) 687, 1066, 1079, 1451, 1532, 2393, 2432
AlwNI CAGNNNCTG 2 cut(s) 977, 2636
Ama87I CYCGRG 1 cut(s) 654
AoxI GGCC 6 cut(s) 52, 230, 974, 1588, 2054, 2336
ApoI RAATTY 5 cut(s) 361, 391, 523, 2571, 3039
AseI ATTAAT 2 cut(s) 1272, 1770
Asp700I GAANNNNTTC 1 cut(s) 2243
AspLEI GCGC 4 cut(s) 1860, 2092, 3052, 3220
AspS9I GGNCC 6 cut(s) 53, 317, 974, 1315, 1588, 3305
AsuC2I CCSGG 1 cut(s) 1586
AsuHPI GGTGA 2 cut(s) 2279, 2359
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 3 cut(s) 317, 1315, 3305
BaeGI GKGCMC 1 cut(s) 3203
BalI TGGCCA 1 cut(s) 2338
BamHI GGATCC 1 cut(s) 1071
BanI GGYRCC 1 cut(s) 1857
BanII GRGCYC 2 cut(s) 37, 655
BauI CACGAG 2 cut(s) 516, 1518
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 4 cut(s) 167, 655, 970, 2164
BbvCI CCTCAGC 3 cut(s) 1239, 1569, 2991
BceAI ACGGC 1 cut(s) 1736
BciT130I CCWGG 8 cut(s) 972, 1260, 1639, 2198, 2340, 3147, 3167, 3303
BciVI GTATCC 1 cut(s) 756
BclI TGATCA 3 cut(s) 271, 568, 2512
BcnI CCSGG 1 cut(s) 1586
BcoDI GTCTC 5 cut(s) 1662, 1812, 2114, 2591, 2688
BcuI ACTAGT 2 cut(s) 239, 1436
BfaI CTAG 7 cut(s) 122, 240, 963, 1437, 1803, 2301, 2543
BfmI CTRYAG 3 cut(s) 603, 2628, 3309
BfoI RGCGCY 2 cut(s) 1861, 3221
BfuI GTATCC 1 cut(s) 756
BglI GCCNNNNNGGC 1 cut(s) 1264
BlpI GCTNAGC 1 cut(s) 1128
Bme1390I CCNGG 9 cut(s) 972, 1260, 1586, 1639, 2198, 2340, 3147, 3167, 3303
Bme18I GGWCC 3 cut(s) 317, 1315, 3305
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 6 cut(s) 53, 317, 974, 1315, 1588, 3305
BmiI GGNNCC 6 cut(s) 34, 319, 1073, 1589, 1859, 2923
BmrFI CCNGG 9 cut(s) 972, 1260, 1586, 1639, 2198, 2340, 3147, 3167, 3303
BmrI ACTGGG 2 cut(s) 1393, 2707
BmsI GCATC 5 cut(s) 364, 604, 615, 3253, 3325
BmuI ACTGGG 2 cut(s) 1393, 2707
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 732
BpmI CTGGAG 3 cut(s) 954, 2658, 3395
Bpu10I CCTNAGC 4 cut(s) 1159, 1239, 1569, 2991
Bpu1102I GCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 2 cut(s) 1482, 2136
BpuMI CCSGG 1 cut(s) 1586
BsaAI YACGTR 2 cut(s) 1226, 1512
BsaBI GATNNNNATC 1 cut(s) 678
BsaHI GRCGYC 2 cut(s) 1858, 3162
BsaI GGTCTC 1 cut(s) 1812
BsaJI CCNNGG 8 cut(s) 169, 556, 711, 971, 1585, 1637, 3190, 3260
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 5 cut(s) 502, 754, 2334, 3262, 3373
Bse1I ACTGG 4 cut(s) 1073, 1388, 2474, 2702
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 8 cut(s) 972, 1260, 1639, 2198, 2340, 3147, 3167, 3303
BseDI CCNNGG 8 cut(s) 169, 556, 711, 971, 1585, 1637, 3190, 3260
BseGI GGATG 7 cut(s) 355, 589, 630, 856, 961, 2906, 3244
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 5 cut(s) 502, 754, 2334, 3262, 3373
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 9 cut(s) 611, 1142, 1154, 1253, 1476, 1560, 2927, 3005, 3076
BseNI ACTGG 4 cut(s) 1073, 1388, 2474, 2702
BseRI GAGGAG 4 cut(s) 1227, 1485, 1586, 3336
BseSI GKGCMC 1 cut(s) 3203
BsgI GTGCAG 5 cut(s) 99, 741, 960, 1635, 1770
Bsh1236I CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
BshFI GGCC 6 cut(s) 54, 232, 976, 1590, 2056, 2338
BshNI GGYRCC 1 cut(s) 1857
BsiHKAI GWGCWC 4 cut(s) 167, 655, 970, 2164
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 2 cut(s) 132, 1585
BslFI GGGAC 4 cut(s) 26, 303, 1103, 1715
BslI CCNNNNNNNGG 5 cut(s) 502, 754, 2334, 3262, 3373
BsmAI GTCTC 5 cut(s) 1662, 1812, 2114, 2591, 2688
BsmBI CGTCTC 1 cut(s) 1662
BsmFI GGGAC 4 cut(s) 26, 303, 1103, 1715
BsmI GAATGC 2 cut(s) 1304, 2716
BsnI GGCC 6 cut(s) 54, 232, 976, 1590, 2056, 2338
Bso31I GGTCTC 1 cut(s) 1812
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 6 cut(s) 37, 167, 655, 970, 2164, 3203
Bsp1407I TGTACA 1 cut(s) 2562
Bsp1720I GCTNAGC 1 cut(s) 1128
Bsp68I TCGCGA 2 cut(s) 551, 3333
BspACI CCGC 8 cut(s) 259, 1775, 1906, 2178, 2320, 2347, 2732, 3175
BspANI GGCC 6 cut(s) 54, 232, 976, 1590, 2056, 2338
BspCNI CTCAG 9 cut(s) 612, 1141, 1153, 1252, 1475, 1561, 2926, 3004, 3075
BspFNI CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
BspLI GGNNCC 6 cut(s) 34, 319, 1073, 1589, 1859, 2923
BspMAI CTGCAG 2 cut(s) 607, 3313
BspPI GGATC 7 cut(s) 687, 1066, 1079, 1451, 1532, 2393, 2432
BspQI GCTCTTC 2 cut(s) 258, 2364
BspT107I GGYRCC 1 cut(s) 1857
BspTNI GGTCTC 1 cut(s) 1812
BsrBI CCGCTC 1 cut(s) 2349
BsrDI GCAATG 1 cut(s) 783
BsrGI TGTACA 1 cut(s) 2562
BsrI ACTGG 4 cut(s) 1073, 1388, 2474, 2702
BssECI CCNNGG 8 cut(s) 169, 556, 711, 971, 1585, 1637, 3190, 3260
BssNI GRCGYC 2 cut(s) 1858, 3162
BssSI CACGAG 2 cut(s) 516, 1518
BssT1I CCWWGG 3 cut(s) 711, 3190, 3260
Bst2BI CACGAG 2 cut(s) 516, 1518
Bst2UI CCWGG 8 cut(s) 972, 1260, 1639, 2198, 2340, 3147, 3167, 3303
Bst4CI ACNGT 6 cut(s) 799, 1656, 1742, 1828, 2140, 3099
Bst6I CTCTTC 4 cut(s) 258, 1178, 2364, 3190
BstACI GRCGYC 2 cut(s) 1858, 3162
BstAPI GCANNNNNTGC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 2562
BstBAI YACGTR 2 cut(s) 1226, 1512
BstC8I GCNNGC 3 cut(s) 2058, 2774, 2907
BstDSI CCRYGG 2 cut(s) 169, 556
BstF5I GGATG 7 cut(s) 355, 589, 630, 856, 961, 2906, 3244
BstFNI CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
BstH2I RGCGCY 2 cut(s) 1861, 3221
BstHHI GCGC 4 cut(s) 1860, 2092, 3052, 3220
BstMAI GTCTC 5 cut(s) 1662, 1812, 2114, 2591, 2688
BstMWI GCNNNNNNNGC 7 cut(s) 602, 1264, 1574, 1937, 1969, 2832, 2852
BstNI CCWGG 8 cut(s) 972, 1260, 1639, 2198, 2340, 3147, 3167, 3303
BstNSI RCATGY 2 cut(s) 179, 1044
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 9 cut(s) 970, 1258, 1584, 1637, 2196, 2338, 3145, 3165, 3301
BstSFI CTRYAG 3 cut(s) 603, 2628, 3309
BstSLI GKGCMC 1 cut(s) 3203
BstUI CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 3 cut(s) 1071, 1537, 2437
BstYI RGATCY 3 cut(s) 1071, 1537, 2437
BsuI GTATCC 1 cut(s) 756
BsuRI GGCC 6 cut(s) 54, 232, 976, 1590, 2056, 2338
BtgI CCRYGG 2 cut(s) 169, 556
BtgZI GCGATG 1 cut(s) 3348
BtsCI GGATG 7 cut(s) 355, 589, 630, 856, 961, 2906, 3244
BtsI GCAGTG 2 cut(s) 1647, 2872
BtsIMutI CAGTG 9 cut(s) 1647, 2136, 2412, 2488, 2598, 2778, 2872, 3095, 3276
BtuMI TCGCGA 2 cut(s) 551, 3333
Cac8I GCNNGC 3 cut(s) 2058, 2774, 2907
CaiI CAGNNNCTG 2 cut(s) 977, 2636
CfoI GCGC 4 cut(s) 1860, 2092, 3052, 3220
Cfr13I GGNCC 6 cut(s) 53, 317, 974, 1315, 1588, 3305
CseI GACGC 2 cut(s) 1998, 3151
Csp6I GTAC 3 cut(s) 687, 1824, 2563
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
DinI GGCGCC 1 cut(s) 1859
EaeI YGGCCR 1 cut(s) 2336
Eam1104I CTCTTC 4 cut(s) 258, 1178, 2364, 3190
EarI CTCTTC 4 cut(s) 258, 1178, 2364, 3190
Ecl136II GAGCTC 1 cut(s) 653
Eco130I CCWWGG 3 cut(s) 711, 3190, 3260
Eco24I GRGCYC 2 cut(s) 37, 655
Eco31I GGTCTC 1 cut(s) 1812
Eco32I GATATC 2 cut(s) 547, 2581
Eco47I GGWCC 3 cut(s) 317, 1315, 3305
Eco53kI GAGCTC 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 762, 1740
Eco88I CYCGRG 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 653
EcoO109I RGGNCCY 2 cut(s) 974, 1588
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 8 cut(s) 970, 1258, 1637, 2196, 2338, 3145, 3165, 3301
EcoRV GATATC 2 cut(s) 547, 2581
EcoT14I CCWWGG 3 cut(s) 711, 3190, 3260
EcoT38I GRGCYC 2 cut(s) 37, 655
EgeI GGCGCC 1 cut(s) 1859
EheI GGCGCC 1 cut(s) 1859
ErhI CCWWGG 3 cut(s) 711, 3190, 3260
Esp3I CGTCTC 1 cut(s) 1662
FaqI GGGAC 4 cut(s) 26, 303, 1103, 1715
FauI CCCGC 4 cut(s) 266, 1913, 2171, 3182
FbaI TGATCA 3 cut(s) 271, 568, 2512
FblI GTMKAC 1 cut(s) 2627
FokI GGATG 7 cut(s) 342, 596, 637, 863, 968, 2913, 3231
FriOI GRGCYC 2 cut(s) 37, 655
FspBI CTAG 7 cut(s) 122, 240, 963, 1437, 1803, 2301, 2543
GlaI GCGC 4 cut(s) 1859, 2091, 3051, 3219
GsuI CTGGAG 3 cut(s) 954, 2658, 3395
HaeII RGCGCY 2 cut(s) 1861, 3221
HaeIII GGCC 6 cut(s) 54, 232, 976, 1590, 2056, 2338
HapII CCGG 2 cut(s) 132, 1585
HgaI GACGC 2 cut(s) 1998, 3151
HhaI GCGC 4 cut(s) 1860, 2092, 3052, 3220
Hin1I GRCGYC 2 cut(s) 1858, 3162
Hin6I GCGC 4 cut(s) 1858, 2090, 3050, 3218
HinP1I GCGC 4 cut(s) 1858, 2090, 3050, 3218
HincII GTYRAC 2 cut(s) 532, 3274
HindII GTYRAC 2 cut(s) 532, 3274
HindIII AAGCTT 2 cut(s) 225, 1005
HpaI GTTAAC 1 cut(s) 3274
HpaII CCGG 2 cut(s) 132, 1585
HphI GGTGA 2 cut(s) 2279, 2359
Hpy166II GTNNAC 5 cut(s) 173, 532, 669, 2628, 3274
Hpy8I GTNNAC 5 cut(s) 173, 532, 669, 2628, 3274
HpyAV CCTTC 7 cut(s) 786, 846, 1601, 2659, 2731, 3040, 3188
HpyCH4III ACNGT 6 cut(s) 799, 1656, 1742, 1828, 2140, 3099
HpyCH4IV ACGT 4 cut(s) 1225, 1511, 1834, 3232
HpyF10VI GCNNNNNNNGC 7 cut(s) 602, 1264, 1574, 1937, 1969, 2832, 2852
HpySE526I ACGT 4 cut(s) 1225, 1511, 1834, 3232
Hsp92I GRCGYC 2 cut(s) 1858, 3162
HspAI GCGC 4 cut(s) 1858, 2090, 3050, 3218
KasI GGCGCC 1 cut(s) 1857
Ksp22I TGATCA 3 cut(s) 271, 568, 2512
KspAI GTTAAC 1 cut(s) 3274
LguI GCTCTTC 2 cut(s) 258, 2364
LmnI GCTCC 7 cut(s) 32, 162, 973, 1401, 1799, 2167, 3083
LweI GCATC 5 cut(s) 364, 604, 615, 3253, 3325
MaeI CTAG 7 cut(s) 122, 240, 963, 1437, 1803, 2301, 2543
MaeII ACGT 4 cut(s) 1225, 1511, 1834, 3232
MaeIII GTNAC 8 cut(s) 424, 880, 1811, 2134, 2140, 2192, 2972, 3149
MbiI CCGCTC 1 cut(s) 2349
MboII GAAGA 9 cut(s) 245, 732, 1052, 1165, 1448, 2083, 2351, 2480, 3177
MfeI CAATTG 3 cut(s) 1152, 1616, 2374
MflI RGATCY 3 cut(s) 1071, 1537, 2437
MhlI GDGCHC 6 cut(s) 37, 167, 655, 970, 2164, 3203
MlsI TGGCCA 1 cut(s) 2338
MluNI TGGCCA 1 cut(s) 2338
Mly113I GGCGCC 1 cut(s) 1858
MlyI GAGTC 6 cut(s) 2116, 2143, 2633, 2858, 2936, 3054
MmeI TCCRAC 7 cut(s) 140, 368, 1421, 2363, 2931, 3361, 3377
Mox20I TGGCCA 1 cut(s) 2338
MroXI GAANNNNTTC 1 cut(s) 2243
MscI TGGCCA 1 cut(s) 2338
MslI CAYNNNNRTG 1 cut(s) 705
Msp20I TGGCCA 1 cut(s) 2338
MspA1I CMGCKG 3 cut(s) 1911, 2219, 3017
MspI CCGG 2 cut(s) 132, 1585
MspR9I CCNGG 9 cut(s) 972, 1260, 1586, 1639, 2198, 2340, 3147, 3167, 3303
MunI CAATTG 3 cut(s) 1152, 1616, 2374
Mva1269I GAATGC 2 cut(s) 1304, 2716
MvaI CCWGG 8 cut(s) 972, 1260, 1639, 2198, 2340, 3147, 3167, 3303
MvnI CGCG 6 cut(s) 261, 551, 2092, 2734, 3052, 3333
MwoI GCNNNNNNNGC 7 cut(s) 602, 1264, 1574, 1937, 1969, 2832, 2852
NarI GGCGCC 1 cut(s) 1858
NciI CCSGG 1 cut(s) 1586
NlaIV GGNNCC 6 cut(s) 34, 319, 1073, 1589, 1859, 2923
NmuCI GTSAC 5 cut(s) 880, 1811, 2134, 2192, 2972
NruI TCGCGA 2 cut(s) 551, 3333
NspI RCATGY 2 cut(s) 179, 1044
PciI ACATGT 1 cut(s) 175
PciSI GCTCTTC 2 cut(s) 258, 2364
PctI GAATGC 2 cut(s) 1304, 2716
PdmI GAANNNNTTC 1 cut(s) 2243
PflMI CCANNNNNTGG 1 cut(s) 3373
PfoI TCCNGGA 1 cut(s) 3165
PleI GAGTC 6 cut(s) 2116, 2143, 2632, 2857, 2936, 3054
PluTI GGCGCC 1 cut(s) 1861
PpsI GAGTC 6 cut(s) 2116, 2143, 2632, 2857, 2936, 3054
Ppu21I YACGTR 2 cut(s) 1226, 1512
PscI ACATGT 1 cut(s) 175
PshAI GACNNNNGTC 1 cut(s) 198
PshBI ATTAAT 2 cut(s) 1272, 1770
PsiI TTATAA 1 cut(s) 3000
Psp124BI GAGCTC 1 cut(s) 655
Psp6I CCWGG 8 cut(s) 970, 1258, 1637, 2196, 2338, 3145, 3165, 3301
PspGI CCWGG 8 cut(s) 970, 1258, 1637, 2196, 2338, 3145, 3165, 3301
PspN4I GGNNCC 6 cut(s) 34, 319, 1073, 1589, 1859, 2923
PspPI GGNCC 6 cut(s) 53, 317, 974, 1315, 1588, 3305
PstI CTGCAG 2 cut(s) 607, 3313
PstNI CAGNNNCTG 2 cut(s) 977, 2636
PsuI RGATCY 3 cut(s) 1071, 1537, 2437
PvuII CAGCTG 3 cut(s) 1911, 2219, 3017
RruI TCGCGA 2 cut(s) 551, 3333
RsaI GTAC 3 cut(s) 688, 1825, 2564
RsaNI GTAC 3 cut(s) 687, 1824, 2563
RseI CAYNNNNRTG 1 cut(s) 705
SacI GAGCTC 1 cut(s) 655
SapI GCTCTTC 2 cut(s) 258, 2364
Sau96I GGNCC 6 cut(s) 53, 317, 974, 1315, 1588, 3305
SchI GAGTC 6 cut(s) 2116, 2143, 2633, 2858, 2936, 3054
ScrFI CCNGG 9 cut(s) 972, 1260, 1586, 1639, 2198, 2340, 3147, 3167, 3303
SduI GDGCHC 6 cut(s) 37, 167, 655, 970, 2164, 3203
SfaNI GCATC 5 cut(s) 364, 604, 615, 3253, 3325
SfcI CTRYAG 3 cut(s) 603, 2628, 3309
SfoI GGCGCC 1 cut(s) 1859
SinI GGWCC 3 cut(s) 317, 1315, 3305
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 2 cut(s) 1497, 2151
SmoI CTYRAG 2 cut(s) 1497, 2151
SpeI ACTAGT 2 cut(s) 239, 1436
SsiI CCGC 8 cut(s) 259, 1775, 1906, 2178, 2320, 2347, 2732, 3175
SspDI GGCGCC 1 cut(s) 1857
SspMI CTAG 7 cut(s) 122, 240, 963, 1437, 1803, 2301, 2543
SstI GAGCTC 1 cut(s) 655
StyD4I CCNGG 9 cut(s) 970, 1258, 1584, 1637, 2196, 2338, 3145, 3165, 3301
StyI CCWWGG 3 cut(s) 711, 3190, 3260
TaaI ACNGT 6 cut(s) 799, 1656, 1742, 1828, 2140, 3099
TaiI ACGT 4 cut(s) 1228, 1514, 1837, 3235
TaqI TCGA 2 cut(s) 2118, 2466
TaqII GACCGA 1 cut(s) 1332
TatI WGTACW 2 cut(s) 686, 2562
TscAI CASTG 9 cut(s) 1654, 2143, 2412, 2488, 2605, 2785, 2879, 3102, 3283
TseFI GTSAC 5 cut(s) 880, 1811, 2134, 2192, 2972
Tsp45I GTSAC 5 cut(s) 880, 1811, 2134, 2192, 2972
TspGWI ACGGA 1 cut(s) 737
TspRI CASTG 9 cut(s) 1654, 2143, 2412, 2488, 2605, 2785, 2879, 3102, 3283
Van91I CCANNNNNTGG 1 cut(s) 3373
VpaK11BI GGWCC 3 cut(s) 317, 1315, 3305
VspI ATTAAT 2 cut(s) 1272, 1770
XapI RAATTY 5 cut(s) 361, 391, 523, 2571, 3039
XbaI TCTAGA 1 cut(s) 121
XceI RCATGY 2 cut(s) 179, 1044
XmiI GTMKAC 1 cut(s) 2627
XmnI GAANNNNTTC 1 cut(s) 2243
XspI CTAG 7 cut(s) 122, 240, 963, 1437, 1803, 2301, 2543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.