Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 21, 32 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 302 |
| Acc36I | ACCTGC | 2 cut(s) | 21, 32 |
| AccBSI | CCGCTC | 1 cut(s) | 1288 |
| AccI | GTMKAC | 1 cut(s) | 1224 |
| AciI | CCGC | 5 cut(s) | 109, 925, 979, 1286, 1320 |
| AclWI | GGATC | 1 cut(s) | 88 |
| AcoI | YGGCCR | 1 cut(s) | 46 |
| AfaI | GTAC | 2 cut(s) | 27, 702 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 799 |
| AflIII | ACRYGT | 1 cut(s) | 410 |
| AgsI | TTSAA | 5 cut(s) | 184, 215, 403, 670, 807 |
| AjiI | CACGTC | 1 cut(s) | 413 |
| AjnI | CCWGG | 3 cut(s) | 915, 1237, 1273 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 103, 135 |
| Alw21I | GWGCWC | 2 cut(s) | 286, 594 |
| Alw26I | GTCTC | 1 cut(s) | 575 |
| Alw44I | GTGCAC | 1 cut(s) | 282 |
| AlwI | GGATC | 1 cut(s) | 88 |
| Ama87I | CYCGRG | 1 cut(s) | 1249 |
| AoxI | GGCC | 3 cut(s) | 46, 1046, 1360 |
| ApaI | GGGCCC | 1 cut(s) | 1364 |
| ApaLI | GTGCAC | 1 cut(s) | 282 |
| ApeKI | GCWGC | 4 cut(s) | 901, 1017, 1073, 1199 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1225, 1257 |
| AseI | ATTAAT | 1 cut(s) | 1301 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 223 |
| AspA2I | CCTAGG | 1 cut(s) | 464 |
| AspLEI | GCGC | 2 cut(s) | 92, 408 |
| AspS9I | GGNCC | 5 cut(s) | 655, 1316, 1351, 1360, 1361 |
| AsuC2I | CCSGG | 1 cut(s) | 1325 |
| AsuHPI | GGTGA | 3 cut(s) | 20, 530, 1324 |
| AvaI | CYCGRG | 1 cut(s) | 1249 |
| AvaII | GGWCC | 3 cut(s) | 655, 1316, 1351 |
| AvrII | CCTAGG | 1 cut(s) | 464 |
| BaeGI | GKGCMC | 2 cut(s) | 286, 1364 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 17, 50 |
| BalI | TGGCCA | 1 cut(s) | 48 |
| BanII | GRGCYC | 1 cut(s) | 1364 |
| BauI | CACGAG | 1 cut(s) | 905 |
| BbsI | GAAGAC | 1 cut(s) | 407 |
| Bbv12I | GWGCWC | 2 cut(s) | 286, 594 |
| BbvI | GCAGC | 4 cut(s) | 913, 1004, 1060, 1211 |
| BccI | CCATC | 3 cut(s) | 61, 362, 889 |
| BciT130I | CCWGG | 3 cut(s) | 917, 1239, 1275 |
| BcnI | CCSGG | 1 cut(s) | 1325 |
| BcoDI | GTCTC | 1 cut(s) | 575 |
| BfaI | CTAG | 3 cut(s) | 453, 465, 780 |
| BfmI | CTRYAG | 3 cut(s) | 585, 827, 1355 |
| BfoI | RGCGCY | 1 cut(s) | 93 |
| BfuAI | ACCTGC | 2 cut(s) | 21, 32 |
| BisI | GCNGC | 4 cut(s) | 902, 1018, 1074, 1200 |
| BlnI | CCTAGG | 1 cut(s) | 464 |
| BlpI | GCTNAGC | 2 cut(s) | 1013, 1132 |
| BlsI | GCNGC | 4 cut(s) | 903, 1019, 1075, 1201 |
| Bme1390I | CCNGG | 4 cut(s) | 917, 1239, 1275, 1325 |
| Bme18I | GGWCC | 3 cut(s) | 655, 1316, 1351 |
| BmeT110I | CYCGRG | 1 cut(s) | 1249 |
| BmgBI | CACGTC | 1 cut(s) | 413 |
| BmgT120I | GGNCC | 5 cut(s) | 655, 1316, 1351, 1360, 1361 |
| BmiI | GGNNCC | 4 cut(s) | 163, 1257, 1318, 1362 |
| BmrFI | CCNGG | 4 cut(s) | 917, 1239, 1275, 1325 |
| BmrI | ACTGGG | 1 cut(s) | 715 |
| BmsI | GCATC | 1 cut(s) | 154 |
| BmuI | ACTGGG | 1 cut(s) | 715 |
| BpiI | GAAGAC | 1 cut(s) | 407 |
| BpmI | CTGGAG | 2 cut(s) | 1221, 1274 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1013, 1132 |
| BpuMI | CCSGG | 1 cut(s) | 1325 |
| BsaJI | CCNNGG | 5 cut(s) | 428, 464, 1273, 1323, 1344 |
| BsaWI | WCCGGW | 1 cut(s) | 697 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 329, 359 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 799 |
| Bse1I | ACTGG | 2 cut(s) | 721, 1291 |
| BseBI | CCWGG | 3 cut(s) | 917, 1239, 1275 |
| BseDI | CCNNGG | 5 cut(s) | 428, 464, 1273, 1323, 1344 |
| BseGI | GGATG | 1 cut(s) | 373 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 799 |
| BseMII | CTCAG | 3 cut(s) | 486, 936, 1004 |
| BseNI | ACTGG | 2 cut(s) | 721, 1291 |
| BseSI | GKGCMC | 2 cut(s) | 286, 1364 |
| BseXI | GCAGC | 4 cut(s) | 913, 1004, 1060, 1211 |
| BseYI | CCCAGC | 2 cut(s) | 157, 249 |
| BsgI | GTGCAG | 1 cut(s) | 920 |
| BshFI | GGCC | 3 cut(s) | 48, 1048, 1362 |
| BsiHKAI | GWGCWC | 2 cut(s) | 286, 594 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1249 |
| BsiSI | CCGG | 2 cut(s) | 698, 1325 |
| BslFI | GGGAC | 1 cut(s) | 308 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 799 |
| BsmAI | GTCTC | 1 cut(s) | 575 |
| BsmFI | GGGAC | 1 cut(s) | 308 |
| BsmI | GAATGC | 2 cut(s) | 448, 790 |
| BsnI | GGCC | 3 cut(s) | 48, 1048, 1362 |
| BsoBI | CYCGRG | 1 cut(s) | 1249 |
| Bsp120I | GGGCCC | 1 cut(s) | 1360 |
| Bsp1286I | GDGCHC | 3 cut(s) | 286, 594, 1364 |
| Bsp143I | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1013, 1132 |
| Bsp19I | CCATGG | 1 cut(s) | 428 |
| BspACI | CCGC | 5 cut(s) | 109, 925, 979, 1286, 1320 |
| BspANI | GGCC | 3 cut(s) | 48, 1048, 1362 |
| BspCNI | CTCAG | 3 cut(s) | 485, 937, 1005 |
| BspLI | GGNNCC | 4 cut(s) | 163, 1257, 1318, 1362 |
| BspMAI | CTGCAG | 2 cut(s) | 589, 1359 |
| BspMI | ACCTGC | 2 cut(s) | 21, 32 |
| BspPI | GGATC | 1 cut(s) | 88 |
| BsrBI | CCGCTC | 1 cut(s) | 1288 |
| BsrI | ACTGG | 2 cut(s) | 721, 1291 |
| BssECI | CCNNGG | 5 cut(s) | 428, 464, 1273, 1323, 1344 |
| BssMI | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| BssSI | CACGAG | 1 cut(s) | 905 |
| BssT1I | CCWWGG | 2 cut(s) | 428, 464 |
| Bst2BI | CACGAG | 1 cut(s) | 905 |
| Bst2UI | CCWGG | 3 cut(s) | 917, 1239, 1275 |
| Bst4CI | ACNGT | 4 cut(s) | 25, 382, 730, 1229 |
| Bst6I | CTCTTC | 1 cut(s) | 602 |
| BstC8I | GCNNGC | 3 cut(s) | 757, 777, 1022 |
| BstDEI | CTNAG | 6 cut(s) | 105, 472, 945, 1013, 1132, 1368 |
| BstDSI | CCRYGG | 1 cut(s) | 428 |
| BstEII | GGTNACC | 1 cut(s) | 536 |
| BstF5I | GGATG | 1 cut(s) | 373 |
| BstH2I | RGCGCY | 1 cut(s) | 93 |
| BstHHI | GCGC | 2 cut(s) | 92, 408 |
| BstKTI | GATC | 5 cut(s) | 6, 83, 178, 887, 951 |
| BstMAI | GTCTC | 1 cut(s) | 575 |
| BstMBI | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 785, 1009, 1070, 1199 |
| BstNI | CCWGG | 3 cut(s) | 917, 1239, 1275 |
| BstNSI | RCATGY | 3 cut(s) | 779, 862, 1085 |
| BstPI | GGTNACC | 1 cut(s) | 536 |
| BstSCI | CCNGG | 4 cut(s) | 915, 1237, 1273, 1323 |
| BstSFI | CTRYAG | 3 cut(s) | 585, 827, 1355 |
| BstSLI | GKGCMC | 2 cut(s) | 286, 1364 |
| BstV1I | GCAGC | 4 cut(s) | 913, 1004, 1060, 1211 |
| BstV2I | GAAGAC | 1 cut(s) | 407 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1044 |
| BsuRI | GGCC | 3 cut(s) | 48, 1048, 1362 |
| BtgI | CCRYGG | 1 cut(s) | 428 |
| BtrI | CACGTC | 1 cut(s) | 413 |
| BtsCI | GGATG | 1 cut(s) | 373 |
| BveI | ACCTGC | 2 cut(s) | 21, 32 |
| Cac8I | GCNNGC | 3 cut(s) | 757, 777, 1022 |
| CfoI | GCGC | 2 cut(s) | 92, 408 |
| Cfr13I | GGNCC | 5 cut(s) | 655, 1316, 1351, 1360, 1361 |
| Csp6I | GTAC | 2 cut(s) | 26, 701 |
| CviAII | CATG | 7 cut(s) | 429, 497, 776, 859, 1033, 1082, 1179 |
| CviQI | GTAC | 2 cut(s) | 26, 701 |
| DdeI | CTNAG | 6 cut(s) | 105, 472, 945, 1013, 1132, 1368 |
| DpnI | GATC | 5 cut(s) | 5, 82, 177, 886, 950 |
| DpnII | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| DraI | TTTAAA | 1 cut(s) | 1218 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 302 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 302 |
| EaeI | YGGCCR | 1 cut(s) | 46 |
| Eam1104I | CTCTTC | 1 cut(s) | 602 |
| EarI | CTCTTC | 1 cut(s) | 602 |
| Eco130I | CCWWGG | 2 cut(s) | 428, 464 |
| Eco24I | GRGCYC | 1 cut(s) | 1364 |
| Eco47I | GGWCC | 3 cut(s) | 655, 1316, 1351 |
| Eco88I | CYCGRG | 1 cut(s) | 1249 |
| Eco91I | GGTNACC | 1 cut(s) | 536 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1316, 1360 |
| EcoO65I | GGTNACC | 1 cut(s) | 536 |
| EcoRII | CCWGG | 3 cut(s) | 915, 1237, 1273 |
| EcoT14I | CCWWGG | 2 cut(s) | 428, 464 |
| EcoT22I | ATGCAT | 1 cut(s) | 1083 |
| EcoT38I | GRGCYC | 1 cut(s) | 1364 |
| ErhI | CCWWGG | 2 cut(s) | 428, 464 |
| FaeI | CATG | 7 cut(s) | 432, 500, 779, 862, 1036, 1085, 1182 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 122, 154 |
| FaqI | GGGAC | 1 cut(s) | 308 |
| FatI | CATG | 7 cut(s) | 428, 496, 775, 858, 1032, 1081, 1178 |
| FauI | CCCGC | 3 cut(s) | 918, 972, 1327 |
| FauNDI | CATATG | 1 cut(s) | 394 |
| FblI | GTMKAC | 1 cut(s) | 1224 |
| Fnu4HI | GCNGC | 4 cut(s) | 902, 1018, 1074, 1200 |
| FokI | GGATG | 1 cut(s) | 380 |
| FriOI | GRGCYC | 1 cut(s) | 1364 |
| Fsp4HI | GCNGC | 4 cut(s) | 902, 1018, 1074, 1200 |
| FspBI | CTAG | 3 cut(s) | 453, 465, 780 |
| GlaI | GCGC | 2 cut(s) | 91, 407 |
| GluI | GCNGC | 4 cut(s) | 902, 1018, 1074, 1200 |
| GsaI | CCCAGC | 2 cut(s) | 161, 253 |
| GsuI | CTGGAG | 2 cut(s) | 1221, 1274 |
| HaeII | RGCGCY | 1 cut(s) | 93 |
| HaeIII | GGCC | 3 cut(s) | 48, 1048, 1362 |
| HapII | CCGG | 2 cut(s) | 698, 1325 |
| HhaI | GCGC | 2 cut(s) | 92, 408 |
| Hin1II | CATG | 7 cut(s) | 432, 500, 779, 862, 1036, 1085, 1182 |
| Hin6I | GCGC | 2 cut(s) | 90, 406 |
| HinP1I | GCGC | 2 cut(s) | 90, 406 |
| HincII | GTYRAC | 3 cut(s) | 726, 1225, 1244 |
| HindII | GTYRAC | 3 cut(s) | 726, 1225, 1244 |
| HindIII | AAGCTT | 1 cut(s) | 131 |
| HinfI | GANTC | 5 cut(s) | 433, 634, 803, 820, 1175 |
| HpaII | CCGG | 2 cut(s) | 698, 1325 |
| HphI | GGTGA | 3 cut(s) | 20, 530, 1324 |
| Hpy166II | GTNNAC | 4 cut(s) | 284, 726, 1225, 1244 |
| Hpy188I | TCNGA | 4 cut(s) | 124, 229, 946, 1345 |
| Hpy188III | TCNNGA | 5 cut(s) | 658, 800, 882, 1106, 1263 |
| Hpy8I | GTNNAC | 4 cut(s) | 284, 726, 1225, 1244 |
| Hpy99I | CGWCG | 2 cut(s) | 534, 1229 |
| HpyAV | CCTTC | 2 cut(s) | 1103, 1269 |
| HpyCH4III | ACNGT | 4 cut(s) | 25, 382, 730, 1229 |
| HpyCH4IV | ACGT | 3 cut(s) | 412, 529, 703 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 785, 1009, 1070, 1199 |
| HpyF3I | CTNAG | 6 cut(s) | 105, 472, 945, 1013, 1132, 1368 |
| HpySE526I | ACGT | 3 cut(s) | 412, 529, 703 |
| Hsp92II | CATG | 7 cut(s) | 432, 500, 779, 862, 1036, 1085, 1182 |
| HspAI | GCGC | 2 cut(s) | 90, 406 |
| Kzo9I | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| LmnI | GCTCC | 4 cut(s) | 563, 722, 837, 1293 |
| Lsp1109I | GCAGC | 4 cut(s) | 913, 1004, 1060, 1211 |
| LweI | GCATC | 1 cut(s) | 154 |
| MaeI | CTAG | 3 cut(s) | 453, 465, 780 |
| MaeII | ACGT | 3 cut(s) | 412, 529, 703 |
| MaeIII | GTNAC | 5 cut(s) | 74, 95, 504, 536, 1309 |
| MalI | GATC | 5 cut(s) | 5, 82, 177, 886, 950 |
| MbiI | CCGCTC | 1 cut(s) | 1288 |
| MboI | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| MboII | GAAGA | 3 cut(s) | 407, 589, 815 |
| MfeI | CAATTG | 1 cut(s) | 1194 |
| MhlI | GDGCHC | 3 cut(s) | 286, 594, 1364 |
| MlsI | TGGCCA | 1 cut(s) | 48 |
| MluNI | TGGCCA | 1 cut(s) | 48 |
| MlyI | GAGTC | 2 cut(s) | 427, 643 |
| MmeI | TCCRAC | 1 cut(s) | 215 |
| Mox20I | TGGCCA | 1 cut(s) | 48 |
| Mph1103I | ATGCAT | 1 cut(s) | 1083 |
| MroXI | GAANNNNTTC | 1 cut(s) | 223 |
| MscI | TGGCCA | 1 cut(s) | 48 |
| MseI | TTAA | 2 cut(s) | 1217, 1301 |
| Msp20I | TGGCCA | 1 cut(s) | 48 |
| MspI | CCGG | 2 cut(s) | 698, 1325 |
| MspR9I | CCNGG | 4 cut(s) | 917, 1239, 1275, 1325 |
| MunI | CAATTG | 1 cut(s) | 1194 |
| Mva1269I | GAATGC | 2 cut(s) | 448, 790 |
| MvaI | CCWGG | 3 cut(s) | 917, 1239, 1275 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 785, 1009, 1070, 1199 |
| NciI | CCSGG | 1 cut(s) | 1325 |
| NcoI | CCATGG | 1 cut(s) | 428 |
| NdeI | CATATG | 1 cut(s) | 394 |
| NdeII | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| NlaIII | CATG | 7 cut(s) | 432, 500, 779, 862, 1036, 1085, 1182 |
| NlaIV | GGNNCC | 4 cut(s) | 163, 1257, 1318, 1362 |
| NmuCI | GTSAC | 3 cut(s) | 74, 536, 1309 |
| NsiI | ATGCAT | 1 cut(s) | 1083 |
| NspI | RCATGY | 3 cut(s) | 779, 862, 1085 |
| PaeI | GCATGC | 1 cut(s) | 779 |
| PaeR7I | CTCGAG | 1 cut(s) | 1249 |
| PaqCI | CACCTGC | 2 cut(s) | 21, 32 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 418, 529 |
| PctI | GAATGC | 2 cut(s) | 448, 790 |
| PdmI | GAANNNNTTC | 1 cut(s) | 223 |
| PfeI | GAWTC | 3 cut(s) | 803, 820, 1175 |
| PflFI | GACNNNGTC | 1 cut(s) | 528 |
| PkrI | GCNGC | 4 cut(s) | 903, 1019, 1075, 1201 |
| PleI | GAGTC | 2 cut(s) | 427, 642 |
| PpsI | GAGTC | 2 cut(s) | 427, 642 |
| PpuMI | RGGWCCY | 1 cut(s) | 1316 |
| PshBI | ATTAAT | 1 cut(s) | 1301 |
| Psp5II | RGGWCCY | 1 cut(s) | 1316 |
| Psp6I | CCWGG | 3 cut(s) | 915, 1237, 1273 |
| PspEI | GGTNACC | 1 cut(s) | 536 |
| PspFI | CCCAGC | 2 cut(s) | 157, 249 |
| PspGI | CCWGG | 3 cut(s) | 915, 1237, 1273 |
| PspN4I | GGNNCC | 4 cut(s) | 163, 1257, 1318, 1362 |
| PspOMI | GGGCCC | 1 cut(s) | 1360 |
| PspPI | GGNCC | 5 cut(s) | 655, 1316, 1351, 1360, 1361 |
| PspPPI | RGGWCCY | 1 cut(s) | 1316 |
| PspXI | VCTCGAGB | 1 cut(s) | 1249 |
| PstI | CTGCAG | 2 cut(s) | 589, 1359 |
| PsyI | GACNNNGTC | 1 cut(s) | 528 |
| RsaI | GTAC | 2 cut(s) | 27, 702 |
| RsaNI | GTAC | 2 cut(s) | 26, 701 |
| SalI | GTCGAC | 1 cut(s) | 1223 |
| SaqAI | TTAA | 2 cut(s) | 1217, 1301 |
| SatI | GCNGC | 4 cut(s) | 902, 1018, 1074, 1200 |
| Sau3AI | GATC | 5 cut(s) | 3, 80, 175, 884, 948 |
| Sau96I | GGNCC | 5 cut(s) | 655, 1316, 1351, 1360, 1361 |
| SbfI | CCTGCAGG | 1 cut(s) | 1359 |
| SchI | GAGTC | 2 cut(s) | 427, 643 |
| ScrFI | CCNGG | 4 cut(s) | 917, 1239, 1275, 1325 |
| SdaI | CCTGCAGG | 1 cut(s) | 1359 |
| SduI | GDGCHC | 3 cut(s) | 286, 594, 1364 |
| SfaNI | GCATC | 1 cut(s) | 154 |
| SfcI | CTRYAG | 3 cut(s) | 585, 827, 1355 |
| Sfr274I | CTCGAG | 1 cut(s) | 1249 |
| SinI | GGWCC | 3 cut(s) | 655, 1316, 1351 |
| SlaI | CTCGAG | 1 cut(s) | 1249 |
| SmiI | ATTTAAAT | 1 cut(s) | 1218 |
| SmlI | CTYRAG | 1 cut(s) | 1249 |
| SmoI | CTYRAG | 1 cut(s) | 1249 |
| SphI | GCATGC | 1 cut(s) | 779 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1359 |
| SsiI | CCGC | 5 cut(s) | 109, 925, 979, 1286, 1320 |
| SspI | AATATT | 1 cut(s) | 187 |
| SspMI | CTAG | 3 cut(s) | 453, 465, 780 |
| StyD4I | CCNGG | 4 cut(s) | 915, 1237, 1273, 1323 |
| StyI | CCWWGG | 2 cut(s) | 428, 464 |
| SwaI | ATTTAAAT | 1 cut(s) | 1218 |
| TaaI | ACNGT | 4 cut(s) | 25, 382, 730, 1229 |
| TaiI | ACGT | 3 cut(s) | 415, 532, 706 |
| TaqI | TCGA | 4 cut(s) | 523, 1224, 1250, 1264 |
| TfiI | GAWTC | 3 cut(s) | 803, 820, 1175 |
| Tru1I | TTAA | 2 cut(s) | 1217, 1301 |
| Tru9I | TTAA | 2 cut(s) | 1217, 1301 |
| TseFI | GTSAC | 3 cut(s) | 74, 536, 1309 |
| TseI | GCWGC | 4 cut(s) | 901, 1017, 1073, 1199 |
| Tsp45I | GTSAC | 3 cut(s) | 74, 536, 1309 |
| TspDTI | ATGAA | 7 cut(s) | 223, 265, 407, 862, 1021, 1154, 1167 |
| TspGWI | ACGGA | 2 cut(s) | 93, 782 |
| Tth111I | GACNNNGTC | 1 cut(s) | 528 |
| VneI | GTGCAC | 1 cut(s) | 282 |
| VpaK11BI | GGWCC | 3 cut(s) | 655, 1316, 1351 |
| VspI | ATTAAT | 1 cut(s) | 1301 |
| XceI | RCATGY | 3 cut(s) | 779, 862, 1085 |
| XhoI | CTCGAG | 1 cut(s) | 1249 |
| XmaJI | CCTAGG | 1 cut(s) | 464 |
| XmiI | GTMKAC | 1 cut(s) | 1224 |
| XmnI | GAANNNNTTC | 1 cut(s) | 223 |
| XspI | CTAG | 3 cut(s) | 453, 465, 780 |
| Zsp2I | ATGCAT | 1 cut(s) | 1083 |