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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 10 |
| AluBI | AGCT | 1 cut(s) | 6 |
| AluI | AGCT | 1 cut(s) | 6 |
| BglII | AGATCT | 1 cut(s) | 24 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 90, 120 |
| BseMII | CTCAG | 1 cut(s) | 146 |
| BseRI | GAGGAG | 1 cut(s) | 98 |
| Bsp143I | GATC | 1 cut(s) | 24 |
| BspCNI | CTCAG | 1 cut(s) | 145 |
| BssMI | GATC | 1 cut(s) | 24 |
| BstDEI | CTNAG | 1 cut(s) | 132 |
| BstKTI | GATC | 1 cut(s) | 27 |
| BstMBI | GATC | 1 cut(s) | 24 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 73 |
| BstX2I | RGATCY | 1 cut(s) | 24 |
| BstYI | RGATCY | 1 cut(s) | 24 |
| BtsI | GCAGTG | 1 cut(s) | 98 |
| BtsIMutI | CAGTG | 1 cut(s) | 98 |
| CviAII | CATG | 1 cut(s) | 172 |
| CviJI | RGCY | 2 cut(s) | 6, 50 |
| CviKI_1 | RGCY | 2 cut(s) | 6, 50 |
| DdeI | CTNAG | 1 cut(s) | 132 |
| DpnI | GATC | 1 cut(s) | 26 |
| DpnII | GATC | 1 cut(s) | 24 |
| FaeI | CATG | 1 cut(s) | 175 |
| FaiI | YATR | 3 cut(s) | 148, 173, 197 |
| FatI | CATG | 1 cut(s) | 171 |
| Hin1II | CATG | 1 cut(s) | 175 |
| HinfI | GANTC | 1 cut(s) | 192 |
| Hpy188I | TCNGA | 2 cut(s) | 19, 135 |
| Hpy188III | TCNNGA | 1 cut(s) | 28 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 73 |
| HpyF3I | CTNAG | 1 cut(s) | 132 |
| Hsp92II | CATG | 1 cut(s) | 175 |
| Kzo9I | GATC | 1 cut(s) | 24 |
| MalI | GATC | 1 cut(s) | 26 |
| MboI | GATC | 1 cut(s) | 24 |
| MflI | RGATCY | 1 cut(s) | 24 |
| MluCI | AATT | 4 cut(s) | 11, 36, 178, 198 |
| MnlI | CCTC | 2 cut(s) | 114, 119 |
| MseI | TTAA | 2 cut(s) | 153, 177 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 73 |
| NdeII | GATC | 1 cut(s) | 24 |
| NlaIII | CATG | 1 cut(s) | 175 |
| PfeI | GAWTC | 1 cut(s) | 192 |
| PsuI | RGATCY | 1 cut(s) | 24 |
| SaqAI | TTAA | 2 cut(s) | 153, 177 |
| Sau3AI | GATC | 1 cut(s) | 24 |
| SetI | ASST | 3 cut(s) | 8, 62, 159 |
| SgeI | CNNG | 3 cut(s) | 19, 40, 184 |
| Sse9I | AATT | 4 cut(s) | 11, 36, 178, 198 |
| TasI | AATT | 4 cut(s) | 11, 36, 178, 198 |
| TfiI | GAWTC | 1 cut(s) | 192 |
| Tru1I | TTAA | 2 cut(s) | 153, 177 |
| Tru9I | TTAA | 2 cut(s) | 153, 177 |
| TscAI | CASTG | 1 cut(s) | 105 |
| TspDTI | ATGAA | 3 cut(s) | 47, 160, 184 |
| TspRI | CASTG | 1 cut(s) | 105 |