Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 248 |
| Acc36I | ACCTGC | 3 cut(s) | 252, 601, 1555 |
| AccB1I | GGYRCC | 1 cut(s) | 1175 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 267 |
| AccI | GTMKAC | 1 cut(s) | 1853 |
| AccII | CGCG | 2 cut(s) | 1145, 1872 |
| AciI | CCGC | 9 cut(s) | 547, 550, 641, 1053, 1058, 1145, 1181, 1202, 1849 |
| AclI | AACGTT | 1 cut(s) | 846 |
| AclWI | GGATC | 5 cut(s) | 112, 336, 348, 1889, 1902 |
| AcoI | YGGCCR | 2 cut(s) | 810, 1237 |
| AcsI | RAATTY | 3 cut(s) | 1205, 1330, 1484 |
| AcuI | CTGAAG | 2 cut(s) | 171, 1535 |
| AcyI | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| AfaI | GTAC | 3 cut(s) | 230, 628, 1372 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 267, 725, 1075, 1529, 1624 |
| AgsI | TTSAA | 5 cut(s) | 404, 1211, 1721, 1732, 1910 |
| AhlI | ACTAGT | 1 cut(s) | 914 |
| AjnI | CCWGG | 2 cut(s) | 324, 1268 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 299, 331, 1284, 1316 |
| Alw21I | GWGCWC | 4 cut(s) | 8, 1159, 1438, 1479 |
| Alw26I | GTCTC | 4 cut(s) | 94, 209, 434, 593 |
| AlwI | GGATC | 5 cut(s) | 112, 336, 348, 1889, 1902 |
| Ama87I | CYCGRG | 1 cut(s) | 1623 |
| AoxI | GGCC | 8 cut(s) | 510, 776, 810, 862, 1114, 1237, 1257, 1413 |
| ApeKI | GCWGC | 5 cut(s) | 288, 818, 1055, 1561, 1587 |
| ApoI | RAATTY | 3 cut(s) | 1205, 1330, 1484 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 123, 155 |
| AspLEI | GCGC | 3 cut(s) | 249, 1178, 1872 |
| AspS9I | GGNCC | 5 cut(s) | 659, 863, 1115, 1257, 1354 |
| AsuC2I | CCSGG | 1 cut(s) | 1840 |
| AsuHPI | GGTGA | 5 cut(s) | 284, 866, 1258, 1388, 1811 |
| AsuII | TTCGAA | 1 cut(s) | 1459 |
| AvaI | CYCGRG | 1 cut(s) | 1623 |
| AvaII | GGWCC | 2 cut(s) | 659, 1354 |
| AxyI | CCTNAGG | 1 cut(s) | 1429 |
| BalI | TGGCCA | 2 cut(s) | 812, 1239 |
| BamHI | GGATCC | 1 cut(s) | 1894 |
| BanI | GGYRCC | 1 cut(s) | 1175 |
| BanII | GRGCYC | 6 cut(s) | 8, 52, 1159, 1438, 1688, 1840 |
| BbsI | GAAGAC | 3 cut(s) | 95, 683, 1506 |
| Bbv12I | GWGCWC | 4 cut(s) | 8, 1159, 1438, 1479 |
| BbvI | GCAGC | 5 cut(s) | 300, 805, 1042, 1548, 1599 |
| BccI | CCATC | 5 cut(s) | 119, 509, 851, 1407, 1779 |
| BciT130I | CCWGG | 2 cut(s) | 326, 1270 |
| BciVI | GTATCC | 1 cut(s) | 25 |
| BclI | TGATCA | 2 cut(s) | 943, 1966 |
| BcnI | CCSGG | 1 cut(s) | 1840 |
| BcoDI | GTCTC | 4 cut(s) | 94, 209, 434, 593 |
| BcuI | ACTAGT | 1 cut(s) | 914 |
| BfaI | CTAG | 4 cut(s) | 915, 1124, 1151, 1776 |
| BfmI | CTRYAG | 2 cut(s) | 486, 1562 |
| BfoI | RGCGCY | 1 cut(s) | 1179 |
| BfuAI | ACCTGC | 3 cut(s) | 252, 601, 1555 |
| BfuI | GTATCC | 1 cut(s) | 25 |
| BglII | AGATCT | 1 cut(s) | 1936 |
| BisI | GCNGC | 7 cut(s) | 289, 547, 550, 819, 1056, 1562, 1588 |
| BlsI | GCNGC | 7 cut(s) | 290, 548, 551, 820, 1057, 1563, 1589 |
| BmcAI | AGTACT | 2 cut(s) | 230, 628 |
| Bme1390I | CCNGG | 3 cut(s) | 326, 1270, 1840 |
| Bme18I | GGWCC | 2 cut(s) | 659, 1354 |
| BmeT110I | CYCGRG | 1 cut(s) | 1623 |
| BmgT120I | GGNCC | 5 cut(s) | 659, 863, 1115, 1257, 1354 |
| BmrFI | CCNGG | 3 cut(s) | 326, 1270, 1840 |
| BmrI | ACTGGG | 2 cut(s) | 45, 1606 |
| BmsI | GCATC | 6 cut(s) | 74, 300, 823, 871, 1336, 1836 |
| BmuI | ACTGGG | 2 cut(s) | 45, 1606 |
| BoxI | GACNNNNGTC | 1 cut(s) | 426 |
| BpiI | GAAGAC | 3 cut(s) | 95, 683, 1506 |
| BpmI | CTGGAG | 1 cut(s) | 618 |
| Bpu14I | TTCGAA | 1 cut(s) | 1459 |
| BpuMI | CCSGG | 1 cut(s) | 1840 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1398, 1789 |
| BsaHI | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| BsaJI | CCNNGG | 7 cut(s) | 17, 44, 336, 813, 1118, 1269, 1291 |
| BsaWI | WCCGGW | 1 cut(s) | 1452 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 267, 725, 1075, 1529, 1624 |
| Bse118I | RCCGGY | 3 cut(s) | 82, 167, 1068 |
| Bse1I | ACTGG | 4 cut(s) | 40, 607, 635, 1601 |
| Bse21I | CCTNAGG | 1 cut(s) | 1429 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1398, 1789 |
| BseBI | CCWGG | 2 cut(s) | 326, 1270 |
| BseDI | CCNNGG | 7 cut(s) | 17, 44, 336, 813, 1118, 1269, 1291 |
| BseGI | GGATG | 4 cut(s) | 979, 1351, 1399, 1851 |
| BseJI | GATNNNNATC | 2 cut(s) | 1398, 1789 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 267, 725, 1075, 1529, 1624 |
| BseMII | CTCAG | 3 cut(s) | 105, 701, 1443 |
| BseNI | ACTGG | 4 cut(s) | 40, 607, 635, 1601 |
| BseRI | GAGGAG | 4 cut(s) | 42, 395, 621, 1013 |
| BseXI | GCAGC | 5 cut(s) | 300, 805, 1042, 1548, 1599 |
| Bsh1236I | CGCG | 2 cut(s) | 1145, 1872 |
| BshFI | GGCC | 8 cut(s) | 512, 778, 812, 864, 1116, 1239, 1259, 1415 |
| BshNI | GGYRCC | 1 cut(s) | 1175 |
| BsiHKAI | GWGCWC | 4 cut(s) | 8, 1159, 1438, 1479 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1623 |
| BsiSI | CCGG | 5 cut(s) | 83, 168, 1069, 1453, 1840 |
| BslFI | GGGAC | 3 cut(s) | 52, 1367, 1521 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 267, 725, 1075, 1529, 1624 |
| BsmAI | GTCTC | 4 cut(s) | 94, 209, 434, 593 |
| BsmBI | CGTCTC | 1 cut(s) | 209 |
| BsmFI | GGGAC | 3 cut(s) | 52, 1367, 1521 |
| BsnI | GGCC | 8 cut(s) | 512, 778, 812, 864, 1116, 1239, 1259, 1415 |
| BsoBI | CYCGRG | 1 cut(s) | 1623 |
| Bsp119I | TTCGAA | 1 cut(s) | 1459 |
| Bsp1286I | GDGCHC | 7 cut(s) | 8, 52, 1159, 1438, 1479, 1688, 1840 |
| Bsp19I | CCATGG | 2 cut(s) | 17, 813 |
| BspACI | CCGC | 9 cut(s) | 547, 550, 641, 1053, 1058, 1145, 1181, 1202, 1849 |
| BspANI | GGCC | 8 cut(s) | 512, 778, 812, 864, 1116, 1239, 1259, 1415 |
| BspCNI | CTCAG | 3 cut(s) | 106, 700, 1442 |
| BspFNI | CGCG | 2 cut(s) | 1145, 1872 |
| BspHI | TCATGA | 2 cut(s) | 946, 1159 |
| BspMAI | CTGCAG | 1 cut(s) | 1566 |
| BspMI | ACCTGC | 3 cut(s) | 252, 601, 1555 |
| BspPI | GGATC | 5 cut(s) | 112, 336, 348, 1889, 1902 |
| BspQI | GCTCTTC | 1 cut(s) | 1587 |
| BspT104I | TTCGAA | 1 cut(s) | 1459 |
| BspT107I | GGYRCC | 1 cut(s) | 1175 |
| BsrFI | RCCGGY | 3 cut(s) | 82, 167, 1068 |
| BsrI | ACTGG | 4 cut(s) | 40, 607, 635, 1601 |
| BssAI | RCCGGY | 3 cut(s) | 82, 167, 1068 |
| BssECI | CCNNGG | 7 cut(s) | 17, 44, 336, 813, 1118, 1269, 1291 |
| BssNI | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| BssT1I | CCWWGG | 5 cut(s) | 17, 44, 813, 1118, 1291 |
| Bst2UI | CCWGG | 2 cut(s) | 326, 1270 |
| Bst4CI | ACNGT | 6 cut(s) | 139, 361, 757, 802, 1036, 1877 |
| Bst6I | CTCTTC | 2 cut(s) | 1587, 2007 |
| BstACI | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1561, 1570 |
| BstBI | TTCGAA | 1 cut(s) | 1459 |
| BstC8I | GCNNGC | 3 cut(s) | 539, 554, 1438 |
| BstDEI | CTNAG | 7 cut(s) | 114, 211, 672, 687, 1380, 1422, 1429 |
| BstDSI | CCRYGG | 2 cut(s) | 17, 813 |
| BstF5I | GGATG | 4 cut(s) | 979, 1351, 1399, 1851 |
| BstFNI | CGCG | 2 cut(s) | 1145, 1872 |
| BstH2I | RGCGCY | 1 cut(s) | 1179 |
| BstHHI | GCGC | 3 cut(s) | 249, 1178, 1872 |
| BstMAI | GTCTC | 4 cut(s) | 94, 209, 434, 593 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 543, 818, 1334, 1561, 1570 |
| BstNI | CCWGG | 2 cut(s) | 326, 1270 |
| BstNSI | RCATGY | 1 cut(s) | 193 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 426 |
| BstSCI | CCNGG | 3 cut(s) | 324, 1268, 1838 |
| BstSFI | CTRYAG | 2 cut(s) | 486, 1562 |
| BstUI | CGCG | 2 cut(s) | 1145, 1872 |
| BstV1I | GCAGC | 5 cut(s) | 300, 805, 1042, 1548, 1599 |
| BstV2I | GAAGAC | 3 cut(s) | 95, 683, 1506 |
| BstX2I | RGATCY | 5 cut(s) | 117, 328, 340, 1894, 1936 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 990 |
| BstYI | RGATCY | 5 cut(s) | 117, 328, 340, 1894, 1936 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1429 |
| BsuI | GTATCC | 1 cut(s) | 25 |
| BsuRI | GGCC | 8 cut(s) | 512, 778, 812, 864, 1116, 1239, 1259, 1415 |
| BtgI | CCRYGG | 2 cut(s) | 17, 813 |
| BtsCI | GGATG | 4 cut(s) | 979, 1351, 1399, 1851 |
| BtsIMutI | CAGTG | 3 cut(s) | 597, 753, 2004 |
| BveI | ACCTGC | 3 cut(s) | 252, 601, 1555 |
| Cac8I | GCNNGC | 3 cut(s) | 539, 554, 1438 |
| CciI | TCATGA | 2 cut(s) | 946, 1159 |
| CfoI | GCGC | 3 cut(s) | 249, 1178, 1872 |
| Cfr10I | RCCGGY | 3 cut(s) | 82, 167, 1068 |
| Cfr13I | GGNCC | 5 cut(s) | 659, 863, 1115, 1257, 1354 |
| CseI | GACGC | 3 cut(s) | 49, 537, 752 |
| Csp6I | GTAC | 3 cut(s) | 229, 627, 1371 |
| CviQI | GTAC | 3 cut(s) | 229, 627, 1371 |
| DdeI | CTNAG | 7 cut(s) | 114, 211, 672, 687, 1380, 1422, 1429 |
| DinI | GGCGCC | 1 cut(s) | 1177 |
| EaeI | YGGCCR | 2 cut(s) | 810, 1237 |
| Eam1104I | CTCTTC | 2 cut(s) | 1587, 2007 |
| EarI | CTCTTC | 2 cut(s) | 1587, 2007 |
| Ecl136II | GAGCTC | 3 cut(s) | 6, 1157, 1436 |
| Eco130I | CCWWGG | 5 cut(s) | 17, 44, 813, 1118, 1291 |
| Eco24I | GRGCYC | 6 cut(s) | 8, 52, 1159, 1438, 1688, 1840 |
| Eco32I | GATATC | 1 cut(s) | 1615 |
| Eco47I | GGWCC | 2 cut(s) | 659, 1354 |
| Eco53kI | GAGCTC | 3 cut(s) | 6, 1157, 1436 |
| Eco57I | CTGAAG | 2 cut(s) | 171, 1535 |
| Eco81I | CCTNAGG | 1 cut(s) | 1429 |
| Eco88I | CYCGRG | 1 cut(s) | 1623 |
| EcoICRI | GAGCTC | 3 cut(s) | 6, 1157, 1436 |
| EcoO109I | RGGNCCY | 2 cut(s) | 659, 1115 |
| EcoRII | CCWGG | 2 cut(s) | 324, 1268 |
| EcoRV | GATATC | 1 cut(s) | 1615 |
| EcoT14I | CCWWGG | 5 cut(s) | 17, 44, 813, 1118, 1291 |
| EcoT22I | ATGCAT | 2 cut(s) | 67, 1648 |
| EcoT38I | GRGCYC | 6 cut(s) | 8, 52, 1159, 1438, 1688, 1840 |
| EgeI | GGCGCC | 1 cut(s) | 1177 |
| EheI | GGCGCC | 1 cut(s) | 1177 |
| ErhI | CCWWGG | 5 cut(s) | 17, 44, 813, 1118, 1291 |
| Esp3I | CGTCTC | 1 cut(s) | 209 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1088, 1120 |
| FaqI | GGGAC | 3 cut(s) | 52, 1367, 1521 |
| FauI | CCCGC | 1 cut(s) | 1188 |
| FbaI | TGATCA | 2 cut(s) | 943, 1966 |
| FblI | GTMKAC | 1 cut(s) | 1853 |
| Fnu4HI | GCNGC | 7 cut(s) | 289, 547, 550, 819, 1056, 1562, 1588 |
| FokI | GGATG | 4 cut(s) | 966, 1358, 1386, 1858 |
| FriOI | GRGCYC | 6 cut(s) | 8, 52, 1159, 1438, 1688, 1840 |
| Fsp4HI | GCNGC | 7 cut(s) | 289, 547, 550, 819, 1056, 1562, 1588 |
| FspBI | CTAG | 4 cut(s) | 915, 1124, 1151, 1776 |
| FspI | TGCGCA | 1 cut(s) | 248 |
| GlaI | GCGC | 3 cut(s) | 248, 1177, 1871 |
| GluI | GCNGC | 7 cut(s) | 289, 547, 550, 819, 1056, 1562, 1588 |
| GsuI | CTGGAG | 1 cut(s) | 618 |
| HaeII | RGCGCY | 1 cut(s) | 1179 |
| HaeIII | GGCC | 8 cut(s) | 512, 778, 812, 864, 1116, 1239, 1259, 1415 |
| HapII | CCGG | 5 cut(s) | 83, 168, 1069, 1453, 1840 |
| HgaI | GACGC | 3 cut(s) | 49, 537, 752 |
| HhaI | GCGC | 3 cut(s) | 249, 1178, 1872 |
| Hin1I | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| Hin6I | GCGC | 3 cut(s) | 247, 1176, 1870 |
| HinP1I | GCGC | 3 cut(s) | 247, 1176, 1870 |
| HincII | GTYRAC | 2 cut(s) | 322, 1190 |
| HindII | GTYRAC | 2 cut(s) | 322, 1190 |
| HindIII | AAGCTT | 1 cut(s) | 470 |
| HinfI | GANTC | 5 cut(s) | 679, 938, 1287, 1321, 1736 |
| HpaII | CCGG | 5 cut(s) | 83, 168, 1069, 1453, 1840 |
| HphI | GGTGA | 5 cut(s) | 284, 866, 1258, 1388, 1811 |
| Hpy166II | GTNNAC | 7 cut(s) | 322, 527, 977, 1190, 1232, 1266, 1854 |
| Hpy188III | TCNNGA | 7 cut(s) | 113, 142, 173, 764, 947, 1160, 1431 |
| Hpy8I | GTNNAC | 7 cut(s) | 322, 527, 977, 1190, 1232, 1266, 1854 |
| Hpy99I | CGWCG | 3 cut(s) | 1068, 1281, 1877 |
| HpyAV | CCTTC | 5 cut(s) | 149, 301, 502, 1426, 1790 |
| HpyCH4III | ACNGT | 6 cut(s) | 139, 361, 757, 802, 1036, 1877 |
| HpyCH4IV | ACGT | 5 cut(s) | 710, 846, 1009, 1063, 1242 |
| HpyCH4V | TGCA | 8 cut(s) | 65, 288, 836, 1328, 1564, 1646, 1750, 1955 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 543, 818, 1334, 1561, 1570 |
| HpyF3I | CTNAG | 7 cut(s) | 114, 211, 672, 687, 1380, 1422, 1429 |
| HpySE526I | ACGT | 5 cut(s) | 710, 846, 1009, 1063, 1242 |
| Hsp92I | GRCGYC | 3 cut(s) | 41, 744, 1176 |
| HspAI | GCGC | 3 cut(s) | 247, 1176, 1870 |
| KasI | GGCGCC | 1 cut(s) | 1175 |
| Ksp22I | TGATCA | 2 cut(s) | 943, 1966 |
| LguI | GCTCTTC | 1 cut(s) | 1587 |
| LmnI | GCTCC | 7 cut(s) | 55, 62, 420, 715, 1154, 1433, 1474 |
| Lsp1109I | GCAGC | 5 cut(s) | 300, 805, 1042, 1548, 1599 |
| LweI | GCATC | 6 cut(s) | 74, 300, 823, 871, 1336, 1836 |
| MaeI | CTAG | 4 cut(s) | 915, 1124, 1151, 1776 |
| MaeII | ACGT | 5 cut(s) | 710, 846, 1009, 1063, 1242 |
| MaeIII | GTNAC | 4 cut(s) | 139, 1015, 1108, 2002 |
| MboII | GAAGA | 9 cut(s) | 95, 164, 656, 688, 1506, 1541, 1574, 2017, 2024 |
| MfeI | CAATTG | 1 cut(s) | 780 |
| MflI | RGATCY | 5 cut(s) | 117, 328, 340, 1894, 1936 |
| MhlI | GDGCHC | 7 cut(s) | 8, 52, 1159, 1438, 1479, 1688, 1840 |
| MlsI | TGGCCA | 2 cut(s) | 812, 1239 |
| MluNI | TGGCCA | 2 cut(s) | 812, 1239 |
| Mly113I | GGCGCC | 1 cut(s) | 1176 |
| MlyI | GAGTC | 3 cut(s) | 673, 1296, 1330 |
| MmeI | TCCRAC | 4 cut(s) | 693, 1200, 1323, 1452 |
| Mox20I | TGGCCA | 2 cut(s) | 812, 1239 |
| Mph1103I | ATGCAT | 2 cut(s) | 67, 1648 |
| MscI | TGGCCA | 2 cut(s) | 812, 1239 |
| MseI | TTAA | 7 cut(s) | 954, 1095, 1140, 1251, 1500, 1641, 1781 |
| MslI | CAYNNNNRTG | 1 cut(s) | 299 |
| Msp20I | TGGCCA | 2 cut(s) | 812, 1239 |
| MspA1I | CMGCKG | 2 cut(s) | 1055, 1590 |
| MspI | CCGG | 5 cut(s) | 83, 168, 1069, 1453, 1840 |
| MspR9I | CCNGG | 3 cut(s) | 326, 1270, 1840 |
| MunI | CAATTG | 1 cut(s) | 780 |
| MvaI | CCWGG | 2 cut(s) | 326, 1270 |
| MvnI | CGCG | 2 cut(s) | 1145, 1872 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 543, 818, 1334, 1561, 1570 |
| NarI | GGCGCC | 1 cut(s) | 1176 |
| NciI | CCSGG | 1 cut(s) | 1840 |
| NcoI | CCATGG | 2 cut(s) | 17, 813 |
| NmeAIII | GCCGAG | 1 cut(s) | 1285 |
| NmuCI | GTSAC | 4 cut(s) | 139, 1015, 1108, 2002 |
| NsbI | TGCGCA | 1 cut(s) | 248 |
| NsiI | ATGCAT | 2 cut(s) | 67, 1648 |
| NspI | RCATGY | 1 cut(s) | 193 |
| NspV | TTCGAA | 1 cut(s) | 1459 |
| PagI | TCATGA | 2 cut(s) | 946, 1159 |
| PciSI | GCTCTTC | 1 cut(s) | 1587 |
| PfeI | GAWTC | 2 cut(s) | 938, 1736 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 267 |
| PkrI | GCNGC | 7 cut(s) | 290, 548, 551, 820, 1057, 1563, 1589 |
| PleI | GAGTC | 3 cut(s) | 673, 1295, 1329 |
| PluTI | GGCGCC | 1 cut(s) | 1179 |
| PpsI | GAGTC | 3 cut(s) | 673, 1295, 1329 |
| PpuMI | RGGWCCY | 1 cut(s) | 659 |
| PshAI | GACNNNNGTC | 1 cut(s) | 426 |
| Psp124BI | GAGCTC | 3 cut(s) | 8, 1159, 1438 |
| Psp1406I | AACGTT | 1 cut(s) | 846 |
| Psp5II | RGGWCCY | 1 cut(s) | 659 |
| Psp6I | CCWGG | 2 cut(s) | 324, 1268 |
| PspGI | CCWGG | 2 cut(s) | 324, 1268 |
| PspPI | GGNCC | 5 cut(s) | 659, 863, 1115, 1257, 1354 |
| PspPPI | RGGWCCY | 1 cut(s) | 659 |
| PstI | CTGCAG | 1 cut(s) | 1566 |
| PsuI | RGATCY | 5 cut(s) | 117, 328, 340, 1894, 1936 |
| PvuII | CAGCTG | 1 cut(s) | 1590 |
| RsaI | GTAC | 3 cut(s) | 230, 628, 1372 |
| RsaNI | GTAC | 3 cut(s) | 229, 627, 1371 |
| RseI | CAYNNNNRTG | 1 cut(s) | 299 |
| SacI | GAGCTC | 3 cut(s) | 8, 1159, 1438 |
| SapI | GCTCTTC | 1 cut(s) | 1587 |
| SaqAI | TTAA | 7 cut(s) | 954, 1095, 1140, 1251, 1500, 1641, 1781 |
| SatI | GCNGC | 7 cut(s) | 289, 547, 550, 819, 1056, 1562, 1588 |
| Sau96I | GGNCC | 5 cut(s) | 659, 863, 1115, 1257, 1354 |
| ScaI | AGTACT | 2 cut(s) | 230, 628 |
| SchI | GAGTC | 3 cut(s) | 673, 1296, 1330 |
| ScrFI | CCNGG | 3 cut(s) | 326, 1270, 1840 |
| SduI | GDGCHC | 7 cut(s) | 8, 52, 1159, 1438, 1479, 1688, 1840 |
| SfaNI | GCATC | 6 cut(s) | 74, 300, 823, 871, 1336, 1836 |
| SfcI | CTRYAG | 2 cut(s) | 486, 1562 |
| SfoI | GGCGCC | 1 cut(s) | 1177 |
| SfuI | TTCGAA | 1 cut(s) | 1459 |
| SinI | GGWCC | 2 cut(s) | 659, 1354 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 299 |
| SpeI | ACTAGT | 1 cut(s) | 914 |
| SsiI | CCGC | 9 cut(s) | 547, 550, 641, 1053, 1058, 1145, 1181, 1202, 1849 |
| SspDI | GGCGCC | 1 cut(s) | 1175 |
| SspI | AATATT | 1 cut(s) | 958 |
| SspMI | CTAG | 4 cut(s) | 915, 1124, 1151, 1776 |
| SstI | GAGCTC | 3 cut(s) | 8, 1159, 1438 |
| StyD4I | CCNGG | 3 cut(s) | 324, 1268, 1838 |
| StyI | CCWWGG | 5 cut(s) | 17, 44, 813, 1118, 1291 |
| TaaI | ACNGT | 6 cut(s) | 139, 361, 757, 802, 1036, 1877 |
| TaiI | ACGT | 5 cut(s) | 713, 849, 1012, 1066, 1245 |
| TaqI | TCGA | 5 cut(s) | 8, 396, 868, 1459, 2021 |
| TatI | WGTACW | 3 cut(s) | 228, 626, 1370 |
| TauI | GCSGC | 2 cut(s) | 549, 552 |
| TfiI | GAWTC | 2 cut(s) | 938, 1736 |
| Tru1I | TTAA | 7 cut(s) | 954, 1095, 1140, 1251, 1500, 1641, 1781 |
| Tru9I | TTAA | 7 cut(s) | 954, 1095, 1140, 1251, 1500, 1641, 1781 |
| TscAI | CASTG | 3 cut(s) | 597, 760, 2011 |
| TseFI | GTSAC | 4 cut(s) | 139, 1015, 1108, 2002 |
| TseI | GCWGC | 5 cut(s) | 288, 818, 1055, 1561, 1587 |
| Tsp45I | GTSAC | 4 cut(s) | 139, 1015, 1108, 2002 |
| TspDTI | ATGAA | 8 cut(s) | 423, 944, 1176, 1478, 1665, 1749, 1811, 2025 |
| TspGWI | ACGGA | 1 cut(s) | 755 |
| TspRI | CASTG | 3 cut(s) | 597, 760, 2011 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 267 |
| VpaK11BI | GGWCC | 2 cut(s) | 659, 1354 |
| XapI | RAATTY | 3 cut(s) | 1205, 1330, 1484 |
| XceI | RCATGY | 1 cut(s) | 193 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 215, 754 |
| XmiI | GTMKAC | 1 cut(s) | 1853 |
| XspI | CTAG | 4 cut(s) | 915, 1124, 1151, 1776 |
| ZrmI | AGTACT | 2 cut(s) | 230, 628 |
| Zsp2I | ATGCAT | 2 cut(s) | 67, 1648 |