Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 3 cut(s) | 18, 341, 592 |
| AccI | GTMKAC | 1 cut(s) | 103 |
| AclWI | GGATC | 1 cut(s) | 710 |
| AcoI | YGGCCR | 1 cut(s) | 640 |
| AcsI | RAATTY | 3 cut(s) | 123, 781, 816 |
| AcuI | CTGAAG | 1 cut(s) | 548 |
| AfaI | GTAC | 1 cut(s) | 310 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 18, 147, 341, 592, 733 |
| AgsI | TTSAA | 1 cut(s) | 299 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 110 |
| AjnI | CCWGG | 4 cut(s) | 206, 288, 630, 856 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 37 |
| AluBI | AGCT | 4 cut(s) | 187, 448, 539, 665 |
| AluI | AGCT | 4 cut(s) | 187, 448, 539, 665 |
| Alw21I | GWGCWC | 1 cut(s) | 247 |
| Alw26I | GTCTC | 1 cut(s) | 641 |
| AlwI | GGATC | 1 cut(s) | 710 |
| AoxI | GGCC | 4 cut(s) | 44, 343, 399, 640 |
| ApeKI | GCWGC | 1 cut(s) | 506 |
| ApoI | RAATTY | 3 cut(s) | 123, 781, 816 |
| AspLEI | GCGC | 1 cut(s) | 609 |
| AspS9I | GGNCC | 3 cut(s) | 203, 266, 860 |
| AsuHPI | GGTGA | 1 cut(s) | 575 |
| AvaII | GGWCC | 3 cut(s) | 203, 266, 860 |
| BaeGI | GKGCMC | 1 cut(s) | 372 |
| BalI | TGGCCA | 1 cut(s) | 642 |
| Bbv12I | GWGCWC | 1 cut(s) | 247 |
| BbvI | GCAGC | 1 cut(s) | 493 |
| BccI | CCATC | 4 cut(s) | 92, 149, 632, 860 |
| BciT130I | CCWGG | 4 cut(s) | 208, 290, 632, 858 |
| BclI | TGATCA | 1 cut(s) | 67 |
| BcoDI | GTCTC | 1 cut(s) | 641 |
| BfaI | CTAG | 1 cut(s) | 540 |
| BfoI | RGCGCY | 1 cut(s) | 610 |
| BisI | GCNGC | 1 cut(s) | 507 |
| BlsI | GCNGC | 1 cut(s) | 508 |
| Bme1390I | CCNGG | 4 cut(s) | 208, 290, 632, 858 |
| Bme18I | GGWCC | 3 cut(s) | 203, 266, 860 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 110 |
| BmgT120I | GGNCC | 3 cut(s) | 203, 266, 860 |
| BmiI | GGNNCC | 2 cut(s) | 204, 205 |
| BmrFI | CCNGG | 4 cut(s) | 208, 290, 632, 858 |
| BmsI | GCATC | 2 cut(s) | 25, 77 |
| BpuEI | CTTGAG | 1 cut(s) | 167 |
| BsaJI | CCNNGG | 2 cut(s) | 206, 630 |
| BsaWI | WCCGGW | 1 cut(s) | 862 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 18, 147, 341, 592, 733 |
| Bse1I | ACTGG | 2 cut(s) | 177, 421 |
| Bse3DI | GCAATG | 1 cut(s) | 843 |
| BseBI | CCWGG | 4 cut(s) | 208, 290, 632, 858 |
| BseDI | CCNNGG | 2 cut(s) | 206, 630 |
| BseGI | GGATG | 4 cut(s) | 92, 626, 847, 871 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 18, 147, 341, 592, 733 |
| BseMI | GCAATG | 1 cut(s) | 843 |
| BseMII | CTCAG | 3 cut(s) | 55, 366, 484 |
| BseNI | ACTGG | 2 cut(s) | 177, 421 |
| BseRI | GAGGAG | 2 cut(s) | 475, 716 |
| BseSI | GKGCMC | 1 cut(s) | 372 |
| BseXI | GCAGC | 1 cut(s) | 493 |
| BsgI | GTGCAG | 1 cut(s) | 492 |
| BshFI | GGCC | 4 cut(s) | 46, 345, 401, 642 |
| BsiHKAI | GWGCWC | 1 cut(s) | 247 |
| BsiSI | CCGG | 1 cut(s) | 863 |
| BslFI | GGGAC | 2 cut(s) | 189, 836 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 18, 147, 341, 592, 733 |
| BsmAI | GTCTC | 1 cut(s) | 641 |
| BsmFI | GGGAC | 2 cut(s) | 189, 836 |
| BsnI | GGCC | 4 cut(s) | 46, 345, 401, 642 |
| Bsp1286I | GDGCHC | 2 cut(s) | 247, 372 |
| Bsp143I | GATC | 4 cut(s) | 67, 565, 658, 715 |
| BspANI | GGCC | 4 cut(s) | 46, 345, 401, 642 |
| BspCNI | CTCAG | 3 cut(s) | 54, 367, 483 |
| BspHI | TCATGA | 1 cut(s) | 247 |
| BspLI | GGNNCC | 2 cut(s) | 204, 205 |
| BspPI | GGATC | 1 cut(s) | 710 |
| BsrDI | GCAATG | 1 cut(s) | 843 |
| BsrI | ACTGG | 2 cut(s) | 177, 421 |
| BssECI | CCNNGG | 2 cut(s) | 206, 630 |
| BssMI | GATC | 4 cut(s) | 67, 565, 658, 715 |
| Bst2UI | CCWGG | 4 cut(s) | 208, 290, 632, 858 |
| Bst4CI | ACNGT | 1 cut(s) | 82 |
| BstC8I | GCNNGC | 3 cut(s) | 6, 92, 467 |
| BstDEI | CTNAG | 5 cut(s) | 41, 375, 444, 470, 763 |
| BstF5I | GGATG | 4 cut(s) | 92, 626, 847, 871 |
| BstH2I | RGCGCY | 1 cut(s) | 610 |
| BstHHI | GCGC | 1 cut(s) | 609 |
| BstKTI | GATC | 4 cut(s) | 70, 568, 661, 718 |
| BstMAI | GTCTC | 1 cut(s) | 641 |
| BstMBI | GATC | 4 cut(s) | 67, 565, 658, 715 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 515 |
| BstNI | CCWGG | 4 cut(s) | 208, 290, 632, 858 |
| BstNSI | RCATGY | 2 cut(s) | 94, 469 |
| BstSCI | CCNGG | 4 cut(s) | 206, 288, 630, 856 |
| BstSLI | GKGCMC | 1 cut(s) | 372 |
| BstV1I | GCAGC | 1 cut(s) | 493 |
| BsuRI | GGCC | 4 cut(s) | 46, 345, 401, 642 |
| BtsCI | GGATG | 4 cut(s) | 92, 626, 847, 871 |
| BtsIMutI | CAGTG | 4 cut(s) | 117, 372, 414, 768 |
| Cac8I | GCNNGC | 3 cut(s) | 6, 92, 467 |
| CciI | TCATGA | 1 cut(s) | 247 |
| CfoI | GCGC | 1 cut(s) | 609 |
| Cfr13I | GGNCC | 3 cut(s) | 203, 266, 860 |
| Csp6I | GTAC | 1 cut(s) | 309 |
| CviAII | CATG | 4 cut(s) | 91, 248, 357, 466 |
| CviQI | GTAC | 1 cut(s) | 309 |
| DdeI | CTNAG | 5 cut(s) | 41, 375, 444, 470, 763 |
| DpnI | GATC | 4 cut(s) | 69, 567, 660, 717 |
| DpnII | GATC | 4 cut(s) | 67, 565, 658, 715 |
| DraI | TTTAAA | 1 cut(s) | 697 |
| DriI | GACNNNNNGTC | 1 cut(s) | 110 |
| EaeI | YGGCCR | 1 cut(s) | 640 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 110 |
| Eco32I | GATATC | 1 cut(s) | 502 |
| Eco47I | GGWCC | 3 cut(s) | 203, 266, 860 |
| Eco57I | CTGAAG | 1 cut(s) | 548 |
| EcoO109I | RGGNCCY | 1 cut(s) | 203 |
| EcoRII | CCWGG | 4 cut(s) | 206, 288, 630, 856 |
| EcoRV | GATATC | 1 cut(s) | 502 |
| EcoT22I | ATGCAT | 1 cut(s) | 92 |
| FaeI | CATG | 4 cut(s) | 94, 251, 360, 469 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 636, 668, 703, 735 |
| FaqI | GGGAC | 2 cut(s) | 189, 836 |
| FatI | CATG | 4 cut(s) | 90, 247, 356, 465 |
| FauNDI | CATATG | 1 cut(s) | 193 |
| FbaI | TGATCA | 1 cut(s) | 67 |
| FblI | GTMKAC | 1 cut(s) | 103 |
| Fnu4HI | GCNGC | 1 cut(s) | 507 |
| FokI | GGATG | 4 cut(s) | 99, 613, 854, 878 |
| Fsp4HI | GCNGC | 1 cut(s) | 507 |
| FspBI | CTAG | 1 cut(s) | 540 |
| GlaI | GCGC | 1 cut(s) | 608 |
| GluI | GCNGC | 1 cut(s) | 507 |
| HaeII | RGCGCY | 1 cut(s) | 610 |
| HaeIII | GGCC | 4 cut(s) | 46, 345, 401, 642 |
| HapII | CCGG | 1 cut(s) | 863 |
| HhaI | GCGC | 1 cut(s) | 609 |
| Hin1II | CATG | 4 cut(s) | 94, 251, 360, 469 |
| Hin6I | GCGC | 1 cut(s) | 607 |
| HinP1I | GCGC | 1 cut(s) | 607 |
| HincII | GTYRAC | 1 cut(s) | 104 |
| HindII | GTYRAC | 1 cut(s) | 104 |
| HindIII | AAGCTT | 3 cut(s) | 185, 446, 663 |
| HinfI | GANTC | 1 cut(s) | 649 |
| HpaII | CCGG | 1 cut(s) | 863 |
| HphI | GGTGA | 1 cut(s) | 575 |
| Hpy166II | GTNNAC | 2 cut(s) | 104, 309 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 473, 658 |
| Hpy188III | TCNNGA | 3 cut(s) | 248, 526, 598 |
| Hpy8I | GTNNAC | 2 cut(s) | 104, 309 |
| HpyAV | CCTTC | 3 cut(s) | 523, 715, 729 |
| HpyCH4III | ACNGT | 1 cut(s) | 82 |
| HpyCH4IV | ACGT | 1 cut(s) | 591 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 59, 90, 131, 234, 409, 509, 681 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 515 |
| HpyF3I | CTNAG | 5 cut(s) | 41, 375, 444, 470, 763 |
| HpySE526I | ACGT | 1 cut(s) | 591 |
| Hsp92II | CATG | 4 cut(s) | 94, 251, 360, 469 |
| HspAI | GCGC | 1 cut(s) | 607 |
| KflI | GGGWCCC | 1 cut(s) | 203 |
| Ksp22I | TGATCA | 1 cut(s) | 67 |
| Kzo9I | GATC | 4 cut(s) | 67, 565, 658, 715 |
| LmnI | GCTCC | 3 cut(s) | 462, 528, 707 |
| Lsp1109I | GCAGC | 1 cut(s) | 493 |
| LweI | GCATC | 2 cut(s) | 25, 77 |
| MaeI | CTAG | 1 cut(s) | 540 |
| MaeII | ACGT | 1 cut(s) | 591 |
| MaeIII | GTNAC | 3 cut(s) | 552, 673, 766 |
| MalI | GATC | 4 cut(s) | 69, 567, 660, 717 |
| MboI | GATC | 4 cut(s) | 67, 565, 658, 715 |
| MboII | GAAGA | 2 cut(s) | 647, 777 |
| MhlI | GDGCHC | 2 cut(s) | 247, 372 |
| MlsI | TGGCCA | 1 cut(s) | 642 |
| MluCI | AATT | 7 cut(s) | 123, 229, 324, 428, 475, 781, 816 |
| MluNI | TGGCCA | 1 cut(s) | 642 |
| MlyI | GAGTC | 1 cut(s) | 643 |
| MmeI | TCCRAC | 1 cut(s) | 64 |
| Mox20I | TGGCCA | 1 cut(s) | 642 |
| Mph1103I | ATGCAT | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 642 |
| MseI | TTAA | 3 cut(s) | 228, 323, 696 |
| Msp20I | TGGCCA | 1 cut(s) | 642 |
| MspI | CCGG | 1 cut(s) | 863 |
| MspR9I | CCNGG | 4 cut(s) | 208, 290, 632, 858 |
| MvaI | CCWGG | 4 cut(s) | 208, 290, 632, 858 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 515 |
| NdeI | CATATG | 1 cut(s) | 193 |
| NdeII | GATC | 4 cut(s) | 67, 565, 658, 715 |
| NlaIII | CATG | 4 cut(s) | 94, 251, 360, 469 |
| NlaIV | GGNNCC | 2 cut(s) | 204, 205 |
| NmuCI | GTSAC | 1 cut(s) | 766 |
| NsiI | ATGCAT | 1 cut(s) | 92 |
| NspI | RCATGY | 2 cut(s) | 94, 469 |
| PaeI | GCATGC | 2 cut(s) | 94, 469 |
| PagI | TCATGA | 1 cut(s) | 247 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 18, 341, 592 |
| PfoI | TCCNGGA | 1 cut(s) | 856 |
| PkrI | GCNGC | 1 cut(s) | 508 |
| PleI | GAGTC | 1 cut(s) | 643 |
| PpsI | GAGTC | 1 cut(s) | 643 |
| PpuMI | RGGWCCY | 1 cut(s) | 203 |
| Psp5II | RGGWCCY | 1 cut(s) | 203 |
| Psp6I | CCWGG | 4 cut(s) | 206, 288, 630, 856 |
| PspGI | CCWGG | 4 cut(s) | 206, 288, 630, 856 |
| PspN4I | GGNNCC | 2 cut(s) | 204, 205 |
| PspPI | GGNCC | 3 cut(s) | 203, 266, 860 |
| PspPPI | RGGWCCY | 1 cut(s) | 203 |
| RsaI | GTAC | 1 cut(s) | 310 |
| RsaNI | GTAC | 1 cut(s) | 309 |
| SalI | GTCGAC | 1 cut(s) | 102 |
| SaqAI | TTAA | 3 cut(s) | 228, 323, 696 |
| SatI | GCNGC | 1 cut(s) | 507 |
| Sau3AI | GATC | 4 cut(s) | 67, 565, 658, 715 |
| Sau96I | GGNCC | 3 cut(s) | 203, 266, 860 |
| SchI | GAGTC | 1 cut(s) | 643 |
| ScrFI | CCNGG | 4 cut(s) | 208, 290, 632, 858 |
| SduI | GDGCHC | 2 cut(s) | 247, 372 |
| SfaNI | GCATC | 2 cut(s) | 25, 77 |
| SinI | GGWCC | 3 cut(s) | 203, 266, 860 |
| SmlI | CTYRAG | 1 cut(s) | 182 |
| SmoI | CTYRAG | 1 cut(s) | 182 |
| SphI | GCATGC | 2 cut(s) | 94, 469 |
| Sse9I | AATT | 7 cut(s) | 123, 229, 324, 428, 475, 781, 816 |
| SspMI | CTAG | 1 cut(s) | 540 |
| StyD4I | CCNGG | 4 cut(s) | 206, 288, 630, 856 |
| TaaI | ACNGT | 1 cut(s) | 82 |
| TaiI | ACGT | 1 cut(s) | 594 |
| TaqI | TCGA | 2 cut(s) | 103, 729 |
| TasI | AATT | 7 cut(s) | 123, 229, 324, 428, 475, 781, 816 |
| TatI | WGTACW | 1 cut(s) | 308 |
| Tru1I | TTAA | 3 cut(s) | 228, 323, 696 |
| Tru9I | TTAA | 3 cut(s) | 228, 323, 696 |
| TscAI | CASTG | 4 cut(s) | 124, 379, 421, 775 |
| TseFI | GTSAC | 1 cut(s) | 766 |
| TseI | GCWGC | 1 cut(s) | 506 |
| Tsp45I | GTSAC | 1 cut(s) | 766 |
| TspDTI | ATGAA | 3 cut(s) | 180, 602, 697 |
| TspRI | CASTG | 4 cut(s) | 124, 379, 421, 775 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 18, 341, 592 |
| VpaK11BI | GGWCC | 3 cut(s) | 203, 266, 860 |
| XapI | RAATTY | 3 cut(s) | 123, 781, 816 |
| XceI | RCATGY | 2 cut(s) | 94, 469 |
| XmiI | GTMKAC | 1 cut(s) | 103 |
| XspI | CTAG | 1 cut(s) | 540 |
| Zsp2I | ATGCAT | 1 cut(s) | 92 |