Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 5 |
| AccI | GTMKAC | 1 cut(s) | 318 |
| AccII | CGCG | 1 cut(s) | 875 |
| AciI | CCGC | 6 cut(s) | 542, 816, 839, 875, 893, 968 |
| AclWI | GGATC | 3 cut(s) | 120, 371, 849 |
| AcsI | RAATTY | 1 cut(s) | 456 |
| AfaI | GTAC | 3 cut(s) | 128, 411, 479 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 451, 940 |
| AgsI | TTSAA | 4 cut(s) | 19, 152, 350, 593 |
| AjnI | CCWGG | 2 cut(s) | 22, 960 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 702, 734 |
| AluBI | AGCT | 3 cut(s) | 45, 475, 680 |
| AluI | AGCT | 3 cut(s) | 45, 475, 680 |
| Alw26I | GTCTC | 2 cut(s) | 354, 532 |
| AlwI | GGATC | 3 cut(s) | 120, 371, 849 |
| AoxI | GGCC | 2 cut(s) | 500, 778 |
| ApeKI | GCWGC | 2 cut(s) | 472, 983 |
| ApoI | RAATTY | 1 cut(s) | 456 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 199, 231 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 714 |
| AspS9I | GGNCC | 4 cut(s) | 439, 501, 778, 964 |
| AsuC2I | CCSGG | 1 cut(s) | 263 |
| AsuHPI | GGTGA | 4 cut(s) | 112, 569, 707, 790 |
| AvaII | GGWCC | 2 cut(s) | 439, 964 |
| AxyI | CCTNAGG | 1 cut(s) | 624 |
| BanI | GGYRCC | 1 cut(s) | 5 |
| BanII | GRGCYC | 1 cut(s) | 261 |
| BbsI | GAAGAC | 1 cut(s) | 746 |
| BbvI | GCAGC | 1 cut(s) | 484 |
| BccI | CCATC | 3 cut(s) | 185, 789, 867 |
| BciT130I | CCWGG | 2 cut(s) | 24, 962 |
| BclI | TGATCA | 1 cut(s) | 202 |
| BcnI | CCSGG | 1 cut(s) | 263 |
| BcoDI | GTCTC | 2 cut(s) | 354, 532 |
| BfaI | CTAG | 1 cut(s) | 936 |
| BisI | GCNGC | 3 cut(s) | 473, 876, 984 |
| BlpI | GCTNAGC | 1 cut(s) | 648 |
| BlsI | GCNGC | 3 cut(s) | 474, 877, 985 |
| BmcAI | AGTACT | 1 cut(s) | 128 |
| Bme1390I | CCNGG | 3 cut(s) | 24, 263, 962 |
| Bme18I | GGWCC | 2 cut(s) | 439, 964 |
| BmgT120I | GGNCC | 4 cut(s) | 439, 501, 778, 964 |
| BmiI | GGNNCC | 3 cut(s) | 7, 945, 966 |
| BmrFI | CCNGG | 3 cut(s) | 24, 263, 962 |
| BmrI | ACTGGG | 1 cut(s) | 139 |
| BmsI | GCATC | 3 cut(s) | 178, 395, 589 |
| BmuI | ACTGGG | 1 cut(s) | 139 |
| BpiI | GAAGAC | 1 cut(s) | 746 |
| Bpu10I | CCTNAGC | 1 cut(s) | 738 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 648 |
| BpuEI | CTTGAG | 3 cut(s) | 75, 107, 297 |
| BpuMI | CCSGG | 1 cut(s) | 263 |
| BsaI | GGTCTC | 2 cut(s) | 354, 532 |
| BsaJI | CCNNGG | 3 cut(s) | 261, 949, 960 |
| BsaWI | WCCGGW | 1 cut(s) | 925 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 426, 456, 567, 597 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 451, 940 |
| Bse1I | ACTGG | 1 cut(s) | 134 |
| Bse21I | CCTNAGG | 1 cut(s) | 624 |
| Bse3DI | GCAATG | 2 cut(s) | 9, 575 |
| BseBI | CCWGG | 2 cut(s) | 24, 962 |
| BseDI | CCNNGG | 3 cut(s) | 261, 949, 960 |
| BseGI | GGATG | 4 cut(s) | 604, 781, 859, 956 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 451, 940 |
| BseMI | GCAATG | 2 cut(s) | 9, 575 |
| BseMII | CTCAG | 4 cut(s) | 638, 662, 735, 752 |
| BseNI | ACTGG | 1 cut(s) | 134 |
| BseRI | GAGGAG | 1 cut(s) | 845 |
| BseXI | GCAGC | 1 cut(s) | 484 |
| BseYI | CCCAGC | 2 cut(s) | 45, 889 |
| Bsh1236I | CGCG | 1 cut(s) | 875 |
| BshFI | GGCC | 2 cut(s) | 502, 780 |
| BshNI | GGYRCC | 1 cut(s) | 5 |
| BsiSI | CCGG | 2 cut(s) | 263, 926 |
| BslFI | GGGAC | 2 cut(s) | 158, 950 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 451, 940 |
| BsmAI | GTCTC | 2 cut(s) | 354, 532 |
| BsmFI | GGGAC | 2 cut(s) | 158, 950 |
| BsnI | GGCC | 2 cut(s) | 502, 780 |
| Bso31I | GGTCTC | 2 cut(s) | 354, 532 |
| Bsp1286I | GDGCHC | 1 cut(s) | 261 |
| Bsp143I | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 648 |
| BspACI | CCGC | 6 cut(s) | 542, 816, 839, 875, 893, 968 |
| BspANI | GGCC | 2 cut(s) | 502, 780 |
| BspCNI | CTCAG | 4 cut(s) | 637, 661, 736, 751 |
| BspFNI | CGCG | 1 cut(s) | 875 |
| BspHI | TCATGA | 1 cut(s) | 39 |
| BspLI | GGNNCC | 3 cut(s) | 7, 945, 966 |
| BspPI | GGATC | 3 cut(s) | 120, 371, 849 |
| BspT107I | GGYRCC | 1 cut(s) | 5 |
| BspTNI | GGTCTC | 2 cut(s) | 354, 532 |
| BsrDI | GCAATG | 2 cut(s) | 9, 575 |
| BsrI | ACTGG | 1 cut(s) | 134 |
| BssECI | CCNNGG | 3 cut(s) | 261, 949, 960 |
| BssMI | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| BssT1I | CCWWGG | 1 cut(s) | 949 |
| Bst2UI | CCWGG | 2 cut(s) | 24, 962 |
| Bst4CI | ACNGT | 2 cut(s) | 486, 884 |
| Bst6I | CTCTTC | 1 cut(s) | 588 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 469 |
| BstC8I | GCNNGC | 1 cut(s) | 837 |
| BstDEI | CTNAG | 7 cut(s) | 327, 518, 624, 648, 681, 738, 744 |
| BstEII | GGTNACC | 1 cut(s) | 884 |
| BstF5I | GGATG | 4 cut(s) | 604, 781, 859, 956 |
| BstFNI | CGCG | 1 cut(s) | 875 |
| BstKTI | GATC | 5 cut(s) | 115, 205, 379, 551, 844 |
| BstMAI | GTCTC | 2 cut(s) | 354, 532 |
| BstMBI | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 469, 989 |
| BstNI | CCWGG | 2 cut(s) | 24, 962 |
| BstPI | GGTNACC | 1 cut(s) | 884 |
| BstSCI | CCNGG | 3 cut(s) | 22, 261, 960 |
| BstUI | CGCG | 1 cut(s) | 875 |
| BstV1I | GCAGC | 1 cut(s) | 484 |
| BstV2I | GAAGAC | 1 cut(s) | 746 |
| BstX2I | RGATCY | 1 cut(s) | 112 |
| BstYI | RGATCY | 1 cut(s) | 112 |
| Bsu36I | CCTNAGG | 1 cut(s) | 624 |
| BsuRI | GGCC | 2 cut(s) | 502, 780 |
| BtsCI | GGATG | 4 cut(s) | 604, 781, 859, 956 |
| BtsI | GCAGTG | 1 cut(s) | 369 |
| BtsIMutI | CAGTG | 1 cut(s) | 369 |
| Cac8I | GCNNGC | 1 cut(s) | 837 |
| CciI | TCATGA | 1 cut(s) | 39 |
| Cfr13I | GGNCC | 4 cut(s) | 439, 501, 778, 964 |
| Csp6I | GTAC | 3 cut(s) | 127, 410, 478 |
| CviAII | CATG | 4 cut(s) | 40, 421, 802, 993 |
| CviQI | GTAC | 3 cut(s) | 127, 410, 478 |
| DdeI | CTNAG | 7 cut(s) | 327, 518, 624, 648, 681, 738, 744 |
| DpnI | GATC | 5 cut(s) | 114, 204, 378, 550, 843 |
| DpnII | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| DraI | TTTAAA | 1 cut(s) | 702 |
| Eam1104I | CTCTTC | 1 cut(s) | 588 |
| EarI | CTCTTC | 1 cut(s) | 588 |
| Eco130I | CCWWGG | 1 cut(s) | 949 |
| Eco24I | GRGCYC | 1 cut(s) | 261 |
| Eco31I | GGTCTC | 2 cut(s) | 354, 532 |
| Eco47I | GGWCC | 2 cut(s) | 439, 964 |
| Eco81I | CCTNAGG | 1 cut(s) | 624 |
| Eco91I | GGTNACC | 1 cut(s) | 884 |
| EcoO109I | RGGNCCY | 3 cut(s) | 439, 501, 964 |
| EcoO65I | GGTNACC | 1 cut(s) | 884 |
| EcoRII | CCWGG | 2 cut(s) | 22, 960 |
| EcoT14I | CCWWGG | 1 cut(s) | 949 |
| EcoT22I | ATGCAT | 1 cut(s) | 388 |
| EcoT38I | GRGCYC | 1 cut(s) | 261 |
| ErhI | CCWWGG | 1 cut(s) | 949 |
| FaeI | CATG | 4 cut(s) | 43, 424, 805, 996 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 702, 734 |
| FaqI | GGGAC | 2 cut(s) | 158, 950 |
| FatI | CATG | 4 cut(s) | 39, 420, 801, 992 |
| FauI | CCCGC | 3 cut(s) | 809, 886, 975 |
| FauNDI | CATATG | 1 cut(s) | 733 |
| FbaI | TGATCA | 1 cut(s) | 202 |
| FblI | GTMKAC | 1 cut(s) | 318 |
| Fnu4HI | GCNGC | 3 cut(s) | 473, 876, 984 |
| FokI | GGATG | 4 cut(s) | 611, 768, 846, 943 |
| FriOI | GRGCYC | 1 cut(s) | 261 |
| Fsp4HI | GCNGC | 3 cut(s) | 473, 876, 984 |
| FspBI | CTAG | 1 cut(s) | 936 |
| GluI | GCNGC | 3 cut(s) | 473, 876, 984 |
| GsaI | CCCAGC | 2 cut(s) | 49, 893 |
| HaeIII | GGCC | 2 cut(s) | 502, 780 |
| HapII | CCGG | 2 cut(s) | 263, 926 |
| Hin1II | CATG | 4 cut(s) | 43, 424, 805, 996 |
| HincII | GTYRAC | 3 cut(s) | 74, 904, 931 |
| HindII | GTYRAC | 3 cut(s) | 74, 904, 931 |
| HinfI | GANTC | 4 cut(s) | 36, 120, 514, 662 |
| HpaI | GTTAAC | 1 cut(s) | 904 |
| HpaII | CCGG | 2 cut(s) | 263, 926 |
| HphI | GGTGA | 4 cut(s) | 112, 569, 707, 790 |
| Hpy166II | GTNNAC | 4 cut(s) | 74, 319, 904, 931 |
| Hpy188I | TCNGA | 1 cut(s) | 667 |
| Hpy188III | TCNNGA | 3 cut(s) | 40, 181, 626 |
| Hpy8I | GTNNAC | 4 cut(s) | 74, 319, 904, 931 |
| HpyAV | CCTTC | 3 cut(s) | 627, 663, 982 |
| HpyCH4III | ACNGT | 2 cut(s) | 486, 884 |
| HpyCH4IV | ACGT | 2 cut(s) | 283, 321 |
| HpyCH4V | TGCA | 5 cut(s) | 224, 386, 472, 568, 983 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 469, 989 |
| HpyF3I | CTNAG | 7 cut(s) | 327, 518, 624, 648, 681, 738, 744 |
| HpySE526I | ACGT | 2 cut(s) | 283, 321 |
| Hsp92II | CATG | 4 cut(s) | 43, 424, 805, 996 |
| Ksp22I | TGATCA | 1 cut(s) | 202 |
| KspAI | GTTAAC | 1 cut(s) | 904 |
| Kzo9I | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| LmnI | GCTCC | 2 cut(s) | 575, 832 |
| Lsp1109I | GCAGC | 1 cut(s) | 484 |
| LweI | GCATC | 3 cut(s) | 178, 395, 589 |
| MaeI | CTAG | 1 cut(s) | 936 |
| MaeII | ACGT | 2 cut(s) | 283, 321 |
| MaeIII | GTNAC | 1 cut(s) | 884 |
| MalI | GATC | 5 cut(s) | 114, 204, 378, 550, 843 |
| MboI | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| MboII | GAAGA | 5 cut(s) | 52, 310, 605, 722, 746 |
| MflI | RGATCY | 1 cut(s) | 112 |
| MhlI | GDGCHC | 1 cut(s) | 261 |
| MluCI | AATT | 4 cut(s) | 55, 165, 456, 978 |
| MlyI | GAGTC | 2 cut(s) | 114, 523 |
| Mph1103I | ATGCAT | 1 cut(s) | 388 |
| MroXI | GAANNNNTTC | 1 cut(s) | 714 |
| MseI | TTAA | 3 cut(s) | 488, 701, 903 |
| MslI | CAYNNNNRTG | 1 cut(s) | 732 |
| MspA1I | CMGCKG | 3 cut(s) | 475, 893, 970 |
| MspI | CCGG | 2 cut(s) | 263, 926 |
| MspR9I | CCNGG | 3 cut(s) | 24, 263, 962 |
| MvaI | CCWGG | 2 cut(s) | 24, 962 |
| MvnI | CGCG | 1 cut(s) | 875 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 469, 989 |
| NciI | CCSGG | 1 cut(s) | 263 |
| NdeI | CATATG | 1 cut(s) | 733 |
| NdeII | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| NlaIII | CATG | 4 cut(s) | 43, 424, 805, 996 |
| NlaIV | GGNNCC | 3 cut(s) | 7, 945, 966 |
| NsiI | ATGCAT | 1 cut(s) | 388 |
| PagI | TCATGA | 1 cut(s) | 39 |
| PdmI | GAANNNNTTC | 1 cut(s) | 714 |
| PfeI | GAWTC | 2 cut(s) | 36, 662 |
| PkrI | GCNGC | 3 cut(s) | 474, 877, 985 |
| PleI | GAGTC | 2 cut(s) | 114, 522 |
| PpsI | GAGTC | 2 cut(s) | 114, 522 |
| PpuMI | RGGWCCY | 2 cut(s) | 439, 964 |
| Psp5II | RGGWCCY | 2 cut(s) | 439, 964 |
| Psp6I | CCWGG | 2 cut(s) | 22, 960 |
| PspEI | GGTNACC | 1 cut(s) | 884 |
| PspFI | CCCAGC | 2 cut(s) | 45, 889 |
| PspGI | CCWGG | 2 cut(s) | 22, 960 |
| PspN4I | GGNNCC | 3 cut(s) | 7, 945, 966 |
| PspPI | GGNCC | 4 cut(s) | 439, 501, 778, 964 |
| PspPPI | RGGWCCY | 2 cut(s) | 439, 964 |
| PsuI | RGATCY | 1 cut(s) | 112 |
| PvuII | CAGCTG | 1 cut(s) | 475 |
| RsaI | GTAC | 3 cut(s) | 128, 411, 479 |
| RsaNI | GTAC | 3 cut(s) | 127, 410, 478 |
| RseI | CAYNNNNRTG | 1 cut(s) | 732 |
| SaqAI | TTAA | 3 cut(s) | 488, 701, 903 |
| SatI | GCNGC | 3 cut(s) | 473, 876, 984 |
| Sau3AI | GATC | 5 cut(s) | 112, 202, 376, 548, 841 |
| Sau96I | GGNCC | 4 cut(s) | 439, 501, 778, 964 |
| ScaI | AGTACT | 1 cut(s) | 128 |
| SchI | GAGTC | 2 cut(s) | 114, 523 |
| ScrFI | CCNGG | 3 cut(s) | 24, 263, 962 |
| SduI | GDGCHC | 1 cut(s) | 261 |
| SfaNI | GCATC | 3 cut(s) | 178, 395, 589 |
| SinI | GGWCC | 2 cut(s) | 439, 964 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 732 |
| SmlI | CTYRAG | 3 cut(s) | 90, 122, 312 |
| SmoI | CTYRAG | 3 cut(s) | 90, 122, 312 |
| Sse9I | AATT | 4 cut(s) | 55, 165, 456, 978 |
| SsiI | CCGC | 6 cut(s) | 542, 816, 839, 875, 893, 968 |
| SspMI | CTAG | 1 cut(s) | 936 |
| StyD4I | CCNGG | 3 cut(s) | 22, 261, 960 |
| StyI | CCWWGG | 1 cut(s) | 949 |
| TaaI | ACNGT | 2 cut(s) | 486, 884 |
| TaiI | ACGT | 2 cut(s) | 286, 324 |
| TasI | AATT | 4 cut(s) | 55, 165, 456, 978 |
| TatI | WGTACW | 2 cut(s) | 126, 409 |
| TauI | GCSGC | 1 cut(s) | 878 |
| TfiI | GAWTC | 2 cut(s) | 36, 662 |
| Tru1I | TTAA | 3 cut(s) | 488, 701, 903 |
| Tru9I | TTAA | 3 cut(s) | 488, 701, 903 |
| TscAI | CASTG | 1 cut(s) | 376 |
| TseI | GCWGC | 2 cut(s) | 472, 983 |
| TspDTI | ATGAA | 3 cut(s) | 28, 662, 723 |
| TspGWI | ACGGA | 2 cut(s) | 418, 434 |
| TspRI | CASTG | 1 cut(s) | 376 |
| VpaK11BI | GGWCC | 2 cut(s) | 439, 964 |
| XapI | RAATTY | 1 cut(s) | 456 |
| XmiI | GTMKAC | 1 cut(s) | 318 |
| XmnI | GAANNNNTTC | 1 cut(s) | 714 |
| XspI | CTAG | 1 cut(s) | 936 |
| ZrmI | AGTACT | 1 cut(s) | 128 |
| Zsp2I | ATGCAT | 1 cut(s) | 388 |