Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 868 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 685 |
| Acc36I | ACCTGC | 2 cut(s) | 145, 478 |
| AciI | CCGC | 1 cut(s) | 239 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 755 |
| AgsI | TTSAA | 4 cut(s) | 368, 416, 614, 666 |
| AhlI | ACTAGT | 1 cut(s) | 314 |
| AjnI | CCWGG | 2 cut(s) | 405, 689 |
| AleI | CACNNNNGTG | 1 cut(s) | 512 |
| AluBI | AGCT | 7 cut(s) | 33, 172, 371, 539, 547, 779, 800 |
| AluI | AGCT | 7 cut(s) | 33, 172, 371, 539, 547, 779, 800 |
| Ama87I | CYCGRG | 1 cut(s) | 748 |
| ApeKI | GCWGC | 4 cut(s) | 224, 242, 536, 776 |
| AseI | ATTAAT | 1 cut(s) | 339 |
| AspLEI | GCGC | 1 cut(s) | 178 |
| AspS9I | GGNCC | 3 cut(s) | 637, 687, 758 |
| AsuC2I | CCSGG | 2 cut(s) | 749, 750 |
| AsuHPI | GGTGA | 4 cut(s) | 206, 295, 429, 728 |
| AvaI | CYCGRG | 1 cut(s) | 748 |
| AvaII | GGWCC | 3 cut(s) | 637, 687, 758 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 664, 696 |
| BbsI | GAAGAC | 2 cut(s) | 345, 729 |
| BbvI | GCAGC | 4 cut(s) | 211, 229, 548, 788 |
| BccI | CCATC | 3 cut(s) | 194, 572, 623 |
| BceAI | ACGGC | 1 cut(s) | 374 |
| BciT130I | CCWGG | 2 cut(s) | 407, 691 |
| BcnI | CCSGG | 2 cut(s) | 749, 750 |
| BcuI | ACTAGT | 1 cut(s) | 314 |
| BfaI | CTAG | 5 cut(s) | 300, 315, 771, 782, 801 |
| BfmI | CTRYAG | 1 cut(s) | 735 |
| BfuAI | ACCTGC | 2 cut(s) | 145, 478 |
| BisI | GCNGC | 5 cut(s) | 225, 240, 243, 537, 777 |
| BlsI | GCNGC | 5 cut(s) | 226, 241, 244, 538, 778 |
| Bme1390I | CCNGG | 4 cut(s) | 407, 691, 749, 750 |
| Bme18I | GGWCC | 3 cut(s) | 637, 687, 758 |
| BmeT110I | CYCGRG | 1 cut(s) | 748 |
| BmgT120I | GGNCC | 3 cut(s) | 637, 687, 758 |
| BmiI | GGNNCC | 1 cut(s) | 159 |
| BmrFI | CCNGG | 4 cut(s) | 407, 691, 749, 750 |
| BmsI | GCATC | 5 cut(s) | 211, 580, 781, 836, 837 |
| BpiI | GAAGAC | 2 cut(s) | 345, 729 |
| Bpu10I | CCTNAGC | 1 cut(s) | 57 |
| BpuEI | CTTGAG | 2 cut(s) | 59, 747 |
| BpuMI | CCSGG | 2 cut(s) | 749, 750 |
| Bsa29I | ATCGAT | 1 cut(s) | 576 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 4 cut(s) | 26, 405, 747, 748 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 87, 117 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 755 |
| BseBI | CCWGG | 2 cut(s) | 407, 691 |
| BseCI | ATCGAT | 1 cut(s) | 576 |
| BseDI | CCNNGG | 4 cut(s) | 26, 405, 747, 748 |
| BseGI | GGATG | 1 cut(s) | 742 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 755 |
| BseMII | CTCAG | 2 cut(s) | 321, 423 |
| BseRI | GAGGAG | 2 cut(s) | 659, 844 |
| BseXI | GCAGC | 4 cut(s) | 211, 229, 548, 788 |
| BsgI | GTGCAG | 1 cut(s) | 228 |
| BshVI | ATCGAT | 1 cut(s) | 576 |
| BsiHKCI | CYCGRG | 1 cut(s) | 748 |
| BsiSI | CCGG | 1 cut(s) | 749 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 755 |
| BsoBI | CYCGRG | 1 cut(s) | 748 |
| Bsp143I | GATC | 1 cut(s) | 739 |
| Bsp19I | CCATGG | 1 cut(s) | 26 |
| BspACI | CCGC | 1 cut(s) | 239 |
| BspCNI | CTCAG | 2 cut(s) | 322, 424 |
| BspDI | ATCGAT | 1 cut(s) | 576 |
| BspLI | GGNNCC | 1 cut(s) | 159 |
| BspMI | ACCTGC | 2 cut(s) | 145, 478 |
| BssECI | CCNNGG | 4 cut(s) | 26, 405, 747, 748 |
| BssMI | GATC | 1 cut(s) | 739 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 1 cut(s) | 26 |
| Bst2UI | CCWGG | 2 cut(s) | 407, 691 |
| Bst4CI | ACNGT | 1 cut(s) | 687 |
| Bst6I | CTCTTC | 1 cut(s) | 705 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstC8I | GCNNGC | 3 cut(s) | 170, 174, 807 |
| BstDEI | CTNAG | 3 cut(s) | 57, 330, 432 |
| BstDSI | CCRYGG | 1 cut(s) | 26 |
| BstF5I | GGATG | 1 cut(s) | 742 |
| BstHHI | GCGC | 1 cut(s) | 178 |
| BstKTI | GATC | 1 cut(s) | 742 |
| BstMBI | GATC | 1 cut(s) | 739 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 30, 806 |
| BstNI | CCWGG | 2 cut(s) | 407, 691 |
| BstNSI | RCATGY | 1 cut(s) | 864 |
| BstSCI | CCNGG | 4 cut(s) | 405, 689, 747, 748 |
| BstSFI | CTRYAG | 1 cut(s) | 735 |
| BstV1I | GCAGC | 4 cut(s) | 211, 229, 548, 788 |
| BstV2I | GAAGAC | 2 cut(s) | 345, 729 |
| Bsu15I | ATCGAT | 1 cut(s) | 576 |
| BsuTUI | ATCGAT | 1 cut(s) | 576 |
| BtgI | CCRYGG | 1 cut(s) | 26 |
| BtsCI | GGATG | 1 cut(s) | 742 |
| BtsI | GCAGTG | 1 cut(s) | 613 |
| BtsIMutI | CAGTG | 2 cut(s) | 487, 613 |
| BveI | ACCTGC | 2 cut(s) | 145, 478 |
| Cac8I | GCNNGC | 3 cut(s) | 170, 174, 807 |
| CfoI | GCGC | 1 cut(s) | 178 |
| Cfr13I | GGNCC | 3 cut(s) | 637, 687, 758 |
| Cfr9I | CCCGGG | 1 cut(s) | 748 |
| ClaI | ATCGAT | 1 cut(s) | 576 |
| CviAII | CATG | 4 cut(s) | 27, 374, 641, 861 |
| DdeI | CTNAG | 3 cut(s) | 57, 330, 432 |
| DpnI | GATC | 1 cut(s) | 741 |
| DpnII | GATC | 1 cut(s) | 739 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 685 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 685 |
| Eam1104I | CTCTTC | 1 cut(s) | 705 |
| EarI | CTCTTC | 1 cut(s) | 705 |
| Eco130I | CCWWGG | 1 cut(s) | 26 |
| Eco47I | GGWCC | 3 cut(s) | 637, 687, 758 |
| Eco88I | CYCGRG | 1 cut(s) | 748 |
| EcoRII | CCWGG | 2 cut(s) | 405, 689 |
| EcoT14I | CCWWGG | 1 cut(s) | 26 |
| ErhI | CCWWGG | 1 cut(s) | 26 |
| FaeI | CATG | 4 cut(s) | 30, 377, 644, 864 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 814, 846 |
| FatI | CATG | 4 cut(s) | 26, 373, 640, 860 |
| FauNDI | CATATG | 1 cut(s) | 524 |
| Fnu4HI | GCNGC | 5 cut(s) | 225, 240, 243, 537, 777 |
| FokI | GGATG | 1 cut(s) | 729 |
| Fsp4HI | GCNGC | 5 cut(s) | 225, 240, 243, 537, 777 |
| FspBI | CTAG | 5 cut(s) | 300, 315, 771, 782, 801 |
| GlaI | GCGC | 1 cut(s) | 177 |
| GluI | GCNGC | 5 cut(s) | 225, 240, 243, 537, 777 |
| HapII | CCGG | 1 cut(s) | 749 |
| HhaI | GCGC | 1 cut(s) | 178 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 4 cut(s) | 30, 377, 644, 864 |
| Hin6I | GCGC | 1 cut(s) | 176 |
| HinP1I | GCGC | 1 cut(s) | 176 |
| HincII | GTYRAC | 1 cut(s) | 454 |
| HindII | GTYRAC | 1 cut(s) | 454 |
| HindIII | AAGCTT | 1 cut(s) | 170 |
| HinfI | GANTC | 6 cut(s) | 41, 52, 68, 434, 461, 767 |
| HpaII | CCGG | 1 cut(s) | 749 |
| HphI | GGTGA | 4 cut(s) | 206, 295, 429, 728 |
| Hpy166II | GTNNAC | 1 cut(s) | 454 |
| Hpy188I | TCNGA | 2 cut(s) | 145, 637 |
| Hpy188III | TCNNGA | 3 cut(s) | 300, 764, 782 |
| Hpy8I | GTNNAC | 1 cut(s) | 454 |
| Hpy99I | CGWCG | 1 cut(s) | 10 |
| HpyAV | CCTTC | 1 cut(s) | 280 |
| HpyCH4III | ACNGT | 1 cut(s) | 687 |
| HpyCH4IV | ACGT | 2 cut(s) | 119, 509 |
| HpyCH4V | TGCA | 7 cut(s) | 154, 205, 245, 494, 563, 794, 809 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 30, 806 |
| HpyF3I | CTNAG | 3 cut(s) | 57, 330, 432 |
| HpySE526I | ACGT | 2 cut(s) | 119, 509 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 4 cut(s) | 30, 377, 644, 864 |
| HspAI | GCGC | 1 cut(s) | 176 |
| Kzo9I | GATC | 1 cut(s) | 739 |
| LmnI | GCTCC | 1 cut(s) | 30 |
| LpnPI | CCDG | 9 cut(s) | 27, 140, 392, 419, 473, 480, 676, 703, 762 |
| Lsp1109I | GCAGC | 4 cut(s) | 211, 229, 548, 788 |
| LweI | GCATC | 5 cut(s) | 211, 580, 781, 836, 837 |
| MaeI | CTAG | 5 cut(s) | 300, 315, 771, 782, 801 |
| MaeII | ACGT | 2 cut(s) | 119, 509 |
| MaeIII | GTNAC | 6 cut(s) | 310, 435, 462, 505, 671, 814 |
| MalI | GATC | 1 cut(s) | 741 |
| MboI | GATC | 1 cut(s) | 739 |
| MboII | GAAGA | 4 cut(s) | 345, 542, 722, 734 |
| MluCI | AATT | 5 cut(s) | 336, 444, 501, 558, 786 |
| MlyI | GAGTC | 4 cut(s) | 50, 62, 443, 470 |
| MmeI | TCCRAC | 1 cut(s) | 206 |
| MseI | TTAA | 1 cut(s) | 339 |
| MslI | CAYNNNNRTG | 2 cut(s) | 469, 512 |
| MspI | CCGG | 1 cut(s) | 749 |
| MspR9I | CCNGG | 4 cut(s) | 407, 691, 749, 750 |
| MvaI | CCWGG | 2 cut(s) | 407, 691 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 30, 806 |
| NciI | CCSGG | 2 cut(s) | 749, 750 |
| NcoI | CCATGG | 1 cut(s) | 26 |
| NdeI | CATATG | 1 cut(s) | 524 |
| NdeII | GATC | 1 cut(s) | 739 |
| NlaIII | CATG | 4 cut(s) | 30, 377, 644, 864 |
| NlaIV | GGNNCC | 1 cut(s) | 159 |
| NmuCI | GTSAC | 4 cut(s) | 435, 462, 505, 814 |
| NspI | RCATGY | 1 cut(s) | 864 |
| OliI | CACNNNNGTG | 1 cut(s) | 512 |
| PfeI | GAWTC | 2 cut(s) | 52, 767 |
| PfoI | TCCNGGA | 1 cut(s) | 689 |
| PkrI | GCNGC | 5 cut(s) | 226, 241, 244, 538, 778 |
| PleI | GAGTC | 4 cut(s) | 49, 62, 442, 469 |
| PpsI | GAGTC | 4 cut(s) | 49, 62, 442, 469 |
| PshBI | ATTAAT | 1 cut(s) | 339 |
| PsiI | TTATAA | 1 cut(s) | 868 |
| Psp6I | CCWGG | 2 cut(s) | 405, 689 |
| PspGI | CCWGG | 2 cut(s) | 405, 689 |
| PspN4I | GGNNCC | 1 cut(s) | 159 |
| PspPI | GGNCC | 3 cut(s) | 637, 687, 758 |
| RseI | CAYNNNNRTG | 2 cut(s) | 469, 512 |
| SaqAI | TTAA | 1 cut(s) | 339 |
| SatI | GCNGC | 5 cut(s) | 225, 240, 243, 537, 777 |
| Sau3AI | GATC | 1 cut(s) | 739 |
| Sau96I | GGNCC | 3 cut(s) | 637, 687, 758 |
| SchI | GAGTC | 4 cut(s) | 50, 62, 443, 470 |
| ScrFI | CCNGG | 4 cut(s) | 407, 691, 749, 750 |
| SfaNI | GCATC | 5 cut(s) | 211, 580, 781, 836, 837 |
| SfcI | CTRYAG | 1 cut(s) | 735 |
| SinI | GGWCC | 3 cut(s) | 637, 687, 758 |
| SmaI | CCCGGG | 1 cut(s) | 750 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 469, 512 |
| SmlI | CTYRAG | 2 cut(s) | 38, 762 |
| SmoI | CTYRAG | 2 cut(s) | 38, 762 |
| SpeI | ACTAGT | 1 cut(s) | 314 |
| Sse9I | AATT | 5 cut(s) | 336, 444, 501, 558, 786 |
| SsiI | CCGC | 1 cut(s) | 239 |
| SspMI | CTAG | 5 cut(s) | 300, 315, 771, 782, 801 |
| StyD4I | CCNGG | 4 cut(s) | 405, 689, 747, 748 |
| StyI | CCWWGG | 1 cut(s) | 26 |
| TaaI | ACNGT | 1 cut(s) | 687 |
| TaiI | ACGT | 2 cut(s) | 122, 512 |
| TaqI | TCGA | 3 cut(s) | 50, 576, 677 |
| TasI | AATT | 5 cut(s) | 336, 444, 501, 558, 786 |
| TauI | GCSGC | 1 cut(s) | 242 |
| TfiI | GAWTC | 2 cut(s) | 52, 767 |
| Tru1I | TTAA | 1 cut(s) | 339 |
| Tru9I | TTAA | 1 cut(s) | 339 |
| TscAI | CASTG | 2 cut(s) | 487, 613 |
| TseFI | GTSAC | 4 cut(s) | 435, 462, 505, 814 |
| TseI | GCWGC | 4 cut(s) | 224, 242, 536, 776 |
| Tsp45I | GTSAC | 4 cut(s) | 435, 462, 505, 814 |
| TspDTI | ATGAA | 1 cut(s) | 285 |
| TspMI | CCCGGG | 1 cut(s) | 748 |
| TspRI | CASTG | 2 cut(s) | 487, 613 |
| VpaK11BI | GGWCC | 3 cut(s) | 637, 687, 758 |
| VspI | ATTAAT | 1 cut(s) | 339 |
| XbaI | TCTAGA | 2 cut(s) | 299, 781 |
| XceI | RCATGY | 1 cut(s) | 864 |
| XmaI | CCCGGG | 1 cut(s) | 748 |
| XspI | CTAG | 5 cut(s) | 300, 315, 771, 782, 801 |