Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 6 cut(s) | 68, 199, 466, 778, 833, 890 |
| AclI | AACGTT | 1 cut(s) | 720 |
| AclWI | GGATC | 3 cut(s) | 483, 504, 545 |
| AcoI | YGGCCR | 1 cut(s) | 196 |
| AcsI | RAATTY | 2 cut(s) | 442, 591 |
| AcuI | CTGAAG | 2 cut(s) | 563, 807 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 472, 763 |
| AgsI | TTSAA | 4 cut(s) | 119, 515, 708, 842 |
| AjnI | CCWGG | 1 cut(s) | 844 |
| AluBI | AGCT | 4 cut(s) | 42, 256, 379, 547 |
| AluI | AGCT | 4 cut(s) | 42, 256, 379, 547 |
| Alw26I | GTCTC | 1 cut(s) | 754 |
| AlwI | GGATC | 3 cut(s) | 483, 504, 545 |
| AoxI | GGCC | 7 cut(s) | 3, 166, 196, 269, 577, 818, 909 |
| ApeKI | GCWGC | 5 cut(s) | 39, 221, 253, 379, 714 |
| ApoI | RAATTY | 2 cut(s) | 442, 591 |
| AspS9I | GGNCC | 5 cut(s) | 463, 577, 775, 848, 910 |
| AsuHPI | GGTGA | 5 cut(s) | 51, 288, 417, 604, 822 |
| AvaII | GGWCC | 3 cut(s) | 463, 775, 848 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 759, 792 |
| BbsI | GAAGAC | 1 cut(s) | 820 |
| BbvI | GCAGC | 5 cut(s) | 51, 208, 265, 366, 726 |
| BccI | CCATC | 4 cut(s) | 98, 143, 647, 859 |
| BceAI | ACGGC | 4 cut(s) | 576, 765, 824, 833 |
| BciT130I | CCWGG | 1 cut(s) | 846 |
| BciVI | GTATCC | 1 cut(s) | 760 |
| BcoDI | GTCTC | 1 cut(s) | 754 |
| BfaI | CTAG | 4 cut(s) | 63, 216, 588, 804 |
| BfmI | CTRYAG | 3 cut(s) | 14, 858, 924 |
| BfuI | GTATCC | 1 cut(s) | 760 |
| BisI | GCNGC | 6 cut(s) | 40, 199, 222, 254, 380, 715 |
| BlsI | GCNGC | 6 cut(s) | 41, 200, 223, 255, 381, 716 |
| Bme1390I | CCNGG | 1 cut(s) | 846 |
| Bme18I | GGWCC | 3 cut(s) | 463, 775, 848 |
| BmgT120I | GGNCC | 5 cut(s) | 463, 577, 775, 848, 910 |
| BmiI | GGNNCC | 2 cut(s) | 464, 658 |
| BmrFI | CCNGG | 1 cut(s) | 846 |
| BmsI | GCATC | 3 cut(s) | 167, 399, 836 |
| BpiI | GAAGAC | 1 cut(s) | 820 |
| BpuEI | CTTGAG | 1 cut(s) | 242 |
| BsaAI | YACGTR | 1 cut(s) | 638 |
| BsaI | GGTCTC | 1 cut(s) | 754 |
| BsaJI | CCNNGG | 3 cut(s) | 301, 759, 781 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 472, 763 |
| Bse1I | ACTGG | 4 cut(s) | 110, 227, 290, 777 |
| Bse3DI | GCAATG | 1 cut(s) | 739 |
| BseBI | CCWGG | 1 cut(s) | 846 |
| BseDI | CCNNGG | 3 cut(s) | 301, 759, 781 |
| BseGI | GGATG | 1 cut(s) | 390 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 472, 763 |
| BseMI | GCAATG | 1 cut(s) | 739 |
| BseNI | ACTGG | 4 cut(s) | 110, 227, 290, 777 |
| BseXI | GCAGC | 5 cut(s) | 51, 208, 265, 366, 726 |
| BsgI | GTGCAG | 1 cut(s) | 207 |
| BshFI | GGCC | 7 cut(s) | 5, 168, 198, 271, 579, 820, 911 |
| BslFI | GGGAC | 1 cut(s) | 399 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 472, 763 |
| BsmAI | GTCTC | 1 cut(s) | 754 |
| BsmFI | GGGAC | 1 cut(s) | 399 |
| BsnI | GGCC | 7 cut(s) | 5, 168, 198, 271, 579, 820, 911 |
| Bso31I | GGTCTC | 1 cut(s) | 754 |
| Bsp143I | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| Bsp19I | CCATGG | 1 cut(s) | 759 |
| BspACI | CCGC | 6 cut(s) | 68, 199, 466, 778, 833, 890 |
| BspANI | GGCC | 7 cut(s) | 5, 168, 198, 271, 579, 820, 911 |
| BspLI | GGNNCC | 2 cut(s) | 464, 658 |
| BspMAI | CTGCAG | 1 cut(s) | 928 |
| BspPI | GGATC | 3 cut(s) | 483, 504, 545 |
| BspTNI | GGTCTC | 1 cut(s) | 754 |
| BsrDI | GCAATG | 1 cut(s) | 739 |
| BsrI | ACTGG | 4 cut(s) | 110, 227, 290, 777 |
| BssECI | CCNNGG | 3 cut(s) | 301, 759, 781 |
| BssMI | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| BssT1I | CCWWGG | 2 cut(s) | 301, 759 |
| Bst2UI | CCWGG | 1 cut(s) | 846 |
| Bst4CI | ACNGT | 1 cut(s) | 337 |
| Bst6I | CTCTTC | 2 cut(s) | 114, 302 |
| BstBAI | YACGTR | 1 cut(s) | 638 |
| BstC8I | GCNNGC | 1 cut(s) | 536 |
| BstDSI | CCRYGG | 2 cut(s) | 759, 781 |
| BstF5I | GGATG | 1 cut(s) | 390 |
| BstKTI | GATC | 6 cut(s) | 100, 478, 499, 553, 568, 650 |
| BstMAI | GTCTC | 1 cut(s) | 754 |
| BstMBI | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 76, 396, 544, 817 |
| BstNI | CCWGG | 1 cut(s) | 846 |
| BstNSI | RCATGY | 1 cut(s) | 538 |
| BstSCI | CCNGG | 1 cut(s) | 844 |
| BstSFI | CTRYAG | 3 cut(s) | 14, 858, 924 |
| BstSNI | TACGTA | 1 cut(s) | 638 |
| BstV1I | GCAGC | 5 cut(s) | 51, 208, 265, 366, 726 |
| BstV2I | GAAGAC | 1 cut(s) | 820 |
| BstX2I | RGATCY | 1 cut(s) | 475 |
| BstYI | RGATCY | 1 cut(s) | 475 |
| BsuI | GTATCC | 1 cut(s) | 760 |
| BsuRI | GGCC | 7 cut(s) | 5, 168, 198, 271, 579, 820, 911 |
| BtgI | CCRYGG | 2 cut(s) | 759, 781 |
| BtsCI | GGATG | 1 cut(s) | 390 |
| BtsI | GCAGTG | 1 cut(s) | 870 |
| BtsIMutI | CAGTG | 2 cut(s) | 770, 870 |
| Cac8I | GCNNGC | 1 cut(s) | 536 |
| Cfr13I | GGNCC | 5 cut(s) | 463, 577, 775, 848, 910 |
| CsiI | ACCWGGT | 1 cut(s) | 844 |
| CviAII | CATG | 4 cut(s) | 319, 535, 662, 760 |
| DpnI | GATC | 6 cut(s) | 99, 477, 498, 552, 567, 649 |
| DpnII | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| EaeI | YGGCCR | 1 cut(s) | 196 |
| Eam1104I | CTCTTC | 2 cut(s) | 114, 302 |
| EarI | CTCTTC | 2 cut(s) | 114, 302 |
| EciI | GGCGGA | 2 cut(s) | 767, 879 |
| Eco105I | TACGTA | 1 cut(s) | 638 |
| Eco130I | CCWWGG | 2 cut(s) | 301, 759 |
| Eco147I | AGGCCT | 1 cut(s) | 168 |
| Eco31I | GGTCTC | 1 cut(s) | 754 |
| Eco47I | GGWCC | 3 cut(s) | 463, 775, 848 |
| Eco57I | CTGAAG | 2 cut(s) | 563, 807 |
| EcoO109I | RGGNCCY | 1 cut(s) | 910 |
| EcoRI | GAATTC | 2 cut(s) | 442, 591 |
| EcoRII | CCWGG | 1 cut(s) | 844 |
| EcoT14I | CCWWGG | 2 cut(s) | 301, 759 |
| ErhI | CCWWGG | 2 cut(s) | 301, 759 |
| FaeI | CATG | 4 cut(s) | 322, 538, 665, 763 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 805, 837 |
| FaqI | GGGAC | 1 cut(s) | 399 |
| FatI | CATG | 4 cut(s) | 318, 534, 661, 759 |
| Fnu4HI | GCNGC | 6 cut(s) | 40, 199, 222, 254, 380, 715 |
| FokI | GGATG | 1 cut(s) | 377 |
| Fsp4HI | GCNGC | 6 cut(s) | 40, 199, 222, 254, 380, 715 |
| FspBI | CTAG | 4 cut(s) | 63, 216, 588, 804 |
| GluI | GCNGC | 6 cut(s) | 40, 199, 222, 254, 380, 715 |
| HaeIII | GGCC | 7 cut(s) | 5, 168, 198, 271, 579, 820, 911 |
| Hin1II | CATG | 4 cut(s) | 322, 538, 665, 763 |
| HindIII | AAGCTT | 1 cut(s) | 545 |
| HinfI | GANTC | 5 cut(s) | 106, 412, 643, 686, 734 |
| HphI | GGTGA | 5 cut(s) | 51, 288, 417, 604, 822 |
| Hpy188I | TCNGA | 5 cut(s) | 148, 333, 543, 652, 733 |
| Hpy188III | TCNNGA | 3 cut(s) | 103, 281, 804 |
| Hpy99I | CGWCG | 1 cut(s) | 98 |
| HpyAV | CCTTC | 6 cut(s) | 222, 282, 287, 404, 831, 923 |
| HpyCH4III | ACNGT | 1 cut(s) | 337 |
| HpyCH4IV | ACGT | 2 cut(s) | 637, 720 |
| HpyCH4V | TGCA | 8 cut(s) | 180, 224, 349, 452, 521, 714, 744, 926 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 76, 396, 544, 817 |
| HpySE526I | ACGT | 2 cut(s) | 637, 720 |
| Hsp92II | CATG | 4 cut(s) | 322, 538, 665, 763 |
| Kzo9I | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| LmnI | GCTCC | 2 cut(s) | 327, 880 |
| Lsp1109I | GCAGC | 5 cut(s) | 51, 208, 265, 366, 726 |
| LweI | GCATC | 3 cut(s) | 167, 399, 836 |
| MabI | ACCWGGT | 1 cut(s) | 844 |
| MaeI | CTAG | 4 cut(s) | 63, 216, 588, 804 |
| MaeII | ACGT | 2 cut(s) | 637, 720 |
| MalI | GATC | 6 cut(s) | 99, 477, 498, 552, 567, 649 |
| MboI | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| MboII | GAAGA | 6 cut(s) | 131, 200, 319, 353, 681, 825 |
| MflI | RGATCY | 1 cut(s) | 475 |
| MluCI | AATT | 5 cut(s) | 192, 326, 442, 516, 591 |
| MlyI | GAGTC | 2 cut(s) | 421, 637 |
| MmeI | TCCRAC | 1 cut(s) | 902 |
| MseI | TTAA | 3 cut(s) | 231, 375, 401 |
| MslI | CAYNNNNRTG | 1 cut(s) | 602 |
| MspR9I | CCNGG | 1 cut(s) | 846 |
| MvaI | CCWGG | 1 cut(s) | 846 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 76, 396, 544, 817 |
| NcoI | CCATGG | 1 cut(s) | 759 |
| NdeII | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| NlaIII | CATG | 4 cut(s) | 322, 538, 665, 763 |
| NlaIV | GGNNCC | 2 cut(s) | 464, 658 |
| NspI | RCATGY | 1 cut(s) | 538 |
| PaeI | GCATGC | 1 cut(s) | 538 |
| PceI | AGGCCT | 1 cut(s) | 168 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 643 |
| PfeI | GAWTC | 3 cut(s) | 106, 686, 734 |
| PkrI | GCNGC | 6 cut(s) | 41, 200, 223, 255, 381, 716 |
| PleI | GAGTC | 2 cut(s) | 420, 637 |
| PpsI | GAGTC | 2 cut(s) | 420, 637 |
| Ppu21I | YACGTR | 1 cut(s) | 638 |
| Psp1406I | AACGTT | 1 cut(s) | 720 |
| Psp6I | CCWGG | 1 cut(s) | 844 |
| PspGI | CCWGG | 1 cut(s) | 844 |
| PspN4I | GGNNCC | 2 cut(s) | 464, 658 |
| PspPI | GGNCC | 5 cut(s) | 463, 577, 775, 848, 910 |
| PstI | CTGCAG | 1 cut(s) | 928 |
| PsuI | RGATCY | 1 cut(s) | 475 |
| RseI | CAYNNNNRTG | 1 cut(s) | 602 |
| SaqAI | TTAA | 3 cut(s) | 231, 375, 401 |
| SatI | GCNGC | 6 cut(s) | 40, 199, 222, 254, 380, 715 |
| Sau3AI | GATC | 6 cut(s) | 97, 475, 496, 550, 565, 647 |
| Sau96I | GGNCC | 5 cut(s) | 463, 577, 775, 848, 910 |
| SchI | GAGTC | 2 cut(s) | 421, 637 |
| ScrFI | CCNGG | 1 cut(s) | 846 |
| SexAI | ACCWGGT | 1 cut(s) | 844 |
| SfaNI | GCATC | 3 cut(s) | 167, 399, 836 |
| SfcI | CTRYAG | 3 cut(s) | 14, 858, 924 |
| SinI | GGWCC | 3 cut(s) | 463, 775, 848 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 602 |
| SmlI | CTYRAG | 1 cut(s) | 257 |
| SmoI | CTYRAG | 1 cut(s) | 257 |
| SnaBI | TACGTA | 1 cut(s) | 638 |
| SphI | GCATGC | 1 cut(s) | 538 |
| Sse9I | AATT | 5 cut(s) | 192, 326, 442, 516, 591 |
| SseBI | AGGCCT | 1 cut(s) | 168 |
| SsiI | CCGC | 6 cut(s) | 68, 199, 466, 778, 833, 890 |
| SspI | AATATT | 1 cut(s) | 901 |
| SspMI | CTAG | 4 cut(s) | 63, 216, 588, 804 |
| StuI | AGGCCT | 1 cut(s) | 168 |
| StyD4I | CCNGG | 1 cut(s) | 844 |
| StyI | CCWWGG | 2 cut(s) | 301, 759 |
| TaaI | ACNGT | 1 cut(s) | 337 |
| TaiI | ACGT | 2 cut(s) | 640, 723 |
| TaqI | TCGA | 2 cut(s) | 93, 646 |
| TasI | AATT | 5 cut(s) | 192, 326, 442, 516, 591 |
| TauI | GCSGC | 1 cut(s) | 201 |
| TfiI | GAWTC | 3 cut(s) | 106, 686, 734 |
| Tru1I | TTAA | 3 cut(s) | 231, 375, 401 |
| Tru9I | TTAA | 3 cut(s) | 231, 375, 401 |
| TscAI | CASTG | 2 cut(s) | 777, 877 |
| TseI | GCWGC | 5 cut(s) | 39, 221, 253, 379, 714 |
| TspDTI | ATGAA | 4 cut(s) | 71, 307, 561, 699 |
| TspRI | CASTG | 2 cut(s) | 777, 877 |
| VpaK11BI | GGWCC | 3 cut(s) | 463, 775, 848 |
| XapI | RAATTY | 2 cut(s) | 442, 591 |
| XbaI | TCTAGA | 1 cut(s) | 803 |
| XceI | RCATGY | 1 cut(s) | 538 |
| XspI | CTAG | 4 cut(s) | 63, 216, 588, 804 |