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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AspS9I | GGNCC | 1 cut(s) | 140 |
| AvaII | GGWCC | 1 cut(s) | 140 |
| BauI | CACGAG | 1 cut(s) | 153 |
| BbsI | GAAGAC | 1 cut(s) | 44 |
| BccI | CCATC | 1 cut(s) | 88 |
| BfaI | CTAG | 1 cut(s) | 132 |
| BfmI | CTRYAG | 1 cut(s) | 174 |
| Bme18I | GGWCC | 1 cut(s) | 140 |
| BmgT120I | GGNCC | 1 cut(s) | 140 |
| BmiI | GGNNCC | 1 cut(s) | 141 |
| BmsI | GCATC | 2 cut(s) | 118, 167 |
| BpiI | GAAGAC | 1 cut(s) | 44 |
| BseMII | CTCAG | 1 cut(s) | 60 |
| BseRI | GAGGAG | 2 cut(s) | 153, 156 |
| BsmI | GAATGC | 1 cut(s) | 127 |
| BspCNI | CTCAG | 1 cut(s) | 61 |
| BspLI | GGNNCC | 1 cut(s) | 141 |
| BssSI | CACGAG | 1 cut(s) | 153 |
| Bst2BI | CACGAG | 1 cut(s) | 153 |
| BstDEI | CTNAG | 1 cut(s) | 69 |
| BstSFI | CTRYAG | 1 cut(s) | 174 |
| BstV2I | GAAGAC | 1 cut(s) | 44 |
| BtsI | GCAGTG | 1 cut(s) | 186 |
| BtsIMutI | CAGTG | 1 cut(s) | 186 |
| Cfr13I | GGNCC | 1 cut(s) | 140 |
| CseI | GACGC | 1 cut(s) | 14 |
| DdeI | CTNAG | 1 cut(s) | 69 |
| Eco47I | GGWCC | 1 cut(s) | 140 |
| EcoO109I | RGGNCCY | 1 cut(s) | 140 |
| FspBI | CTAG | 1 cut(s) | 132 |
| HgaI | GACGC | 1 cut(s) | 14 |
| HinfI | GANTC | 1 cut(s) | 191 |
| Hpy166II | GTNNAC | 1 cut(s) | 32 |
| Hpy8I | GTNNAC | 1 cut(s) | 32 |
| Hpy99I | CGWCG | 1 cut(s) | 30 |
| HpyAV | CCTTC | 1 cut(s) | 153 |
| HpyCH4V | TGCA | 2 cut(s) | 59, 125 |
| HpyF3I | CTNAG | 1 cut(s) | 69 |
| LmnI | GCTCC | 1 cut(s) | 109 |
| LweI | GCATC | 2 cut(s) | 118, 167 |
| MaeI | CTAG | 1 cut(s) | 132 |
| MboII | GAAGA | 2 cut(s) | 49, 103 |
| MnlI | CCTC | 3 cut(s) | 130, 174, 177 |
| Mva1269I | GAATGC | 1 cut(s) | 127 |
| NlaIV | GGNNCC | 1 cut(s) | 141 |
| PctI | GAATGC | 1 cut(s) | 127 |
| PfeI | GAWTC | 1 cut(s) | 191 |
| PpuMI | RGGWCCY | 1 cut(s) | 140 |
| Psp5II | RGGWCCY | 1 cut(s) | 140 |
| PspN4I | GGNNCC | 1 cut(s) | 141 |
| PspPI | GGNCC | 1 cut(s) | 140 |
| PspPPI | RGGWCCY | 1 cut(s) | 140 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 13, 45 |
| Sau96I | GGNCC | 1 cut(s) | 140 |
| SetI | ASST | 1 cut(s) | 13 |
| SfaNI | GCATC | 2 cut(s) | 118, 167 |
| SfcI | CTRYAG | 1 cut(s) | 174 |
| SgeI | CNNG | 9 cut(s) | 20, 30, 45, 59, 86, 126, 144, 165, 167 |
| SinI | GGWCC | 1 cut(s) | 140 |
| SspMI | CTAG | 1 cut(s) | 132 |
| TfiI | GAWTC | 1 cut(s) | 191 |
| TscAI | CASTG | 1 cut(s) | 186 |
| TspDTI | ATGAA | 1 cut(s) | 135 |
| TspGWI | ACGGA | 1 cut(s) | 49 |
| TspRI | CASTG | 1 cut(s) | 186 |
| VpaK11BI | GGWCC | 1 cut(s) | 140 |
| XspI | CTAG | 1 cut(s) | 132 |