No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 478 |
| AccB1I | GGYRCC | 2 cut(s) | 28, 190 |
| AciI | CCGC | 2 cut(s) | 32, 188 |
| AcsI | RAATTY | 2 cut(s) | 52, 386 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 493 |
| AgsI | TTSAA | 2 cut(s) | 97, 273 |
| AjiI | CACGTC | 1 cut(s) | 282 |
| AjnI | CCWGG | 2 cut(s) | 229, 264 |
| AluBI | AGCT | 6 cut(s) | 122, 140, 158, 276, 305, 352 |
| AluI | AGCT | 6 cut(s) | 122, 140, 158, 276, 305, 352 |
| Alw21I | GWGCWC | 1 cut(s) | 484 |
| Alw26I | GTCTC | 1 cut(s) | 101 |
| AoxI | GGCC | 1 cut(s) | 475 |
| ApeKI | GCWGC | 3 cut(s) | 119, 185, 302 |
| ApoI | RAATTY | 2 cut(s) | 52, 386 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 331, 363 |
| AsuHPI | GGTGA | 1 cut(s) | 395 |
| AsuNHI | GCTAGC | 1 cut(s) | 500 |
| BanI | GGYRCC | 2 cut(s) | 28, 190 |
| Bbv12I | GWGCWC | 1 cut(s) | 484 |
| BbvI | GCAGC | 3 cut(s) | 131, 172, 314 |
| BceAI | ACGGC | 2 cut(s) | 42, 462 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 403, 437 |
| BciT130I | CCWGG | 2 cut(s) | 231, 266 |
| BcoDI | GTCTC | 1 cut(s) | 101 |
| BfaI | CTAG | 1 cut(s) | 501 |
| BfmI | CTRYAG | 1 cut(s) | 471 |
| BfuAI | ACCTGC | 1 cut(s) | 478 |
| BisI | GCNGC | 3 cut(s) | 120, 186, 303 |
| BlsI | GCNGC | 3 cut(s) | 121, 187, 304 |
| Bme1390I | CCNGG | 2 cut(s) | 231, 266 |
| BmgBI | CACGTC | 1 cut(s) | 282 |
| BmiI | GGNNCC | 3 cut(s) | 30, 192, 249 |
| BmrFI | CCNGG | 2 cut(s) | 231, 266 |
| BmtI | GCTAGC | 1 cut(s) | 504 |
| BpmI | CTGGAG | 3 cut(s) | 126, 144, 248 |
| BpuEI | CTTGAG | 1 cut(s) | 491 |
| BsaBI | GATNNNNATC | 1 cut(s) | 333 |
| BsaJI | CCNNGG | 1 cut(s) | 487 |
| BsaWI | WCCGGW | 1 cut(s) | 250 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 106, 124, 136, 154 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 493 |
| Bse1I | ACTGG | 3 cut(s) | 143, 161, 395 |
| Bse8I | GATNNNNATC | 1 cut(s) | 333 |
| BseBI | CCWGG | 2 cut(s) | 231, 266 |
| BseDI | CCNNGG | 1 cut(s) | 487 |
| BseGI | GGATG | 1 cut(s) | 222 |
| BseJI | GATNNNNATC | 1 cut(s) | 333 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 493 |
| BseMII | CTCAG | 1 cut(s) | 357 |
| BseNI | ACTGG | 3 cut(s) | 143, 161, 395 |
| BseRI | GAGGAG | 1 cut(s) | 297 |
| BseXI | GCAGC | 3 cut(s) | 131, 172, 314 |
| BseYI | CCCAGC | 1 cut(s) | 352 |
| BsgI | GTGCAG | 1 cut(s) | 218 |
| BshFI | GGCC | 1 cut(s) | 477 |
| BshNI | GGYRCC | 2 cut(s) | 28, 190 |
| BsiHKAI | GWGCWC | 1 cut(s) | 484 |
| BsiSI | CCGG | 1 cut(s) | 251 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 493 |
| BsmAI | GTCTC | 1 cut(s) | 101 |
| BsnI | GGCC | 1 cut(s) | 477 |
| Bsp1286I | GDGCHC | 1 cut(s) | 484 |
| BspACI | CCGC | 2 cut(s) | 32, 188 |
| BspANI | GGCC | 1 cut(s) | 477 |
| BspCNI | CTCAG | 1 cut(s) | 358 |
| BspHI | TCATGA | 1 cut(s) | 150 |
| BspLI | GGNNCC | 3 cut(s) | 30, 192, 249 |
| BspMAI | CTGCAG | 1 cut(s) | 475 |
| BspMI | ACCTGC | 1 cut(s) | 478 |
| BspOI | GCTAGC | 1 cut(s) | 504 |
| BspT107I | GGYRCC | 2 cut(s) | 28, 190 |
| BsrI | ACTGG | 3 cut(s) | 143, 161, 395 |
| BssECI | CCNNGG | 1 cut(s) | 487 |
| BssT1I | CCWWGG | 1 cut(s) | 487 |
| Bst2UI | CCWGG | 2 cut(s) | 231, 266 |
| Bst4CI | ACNGT | 1 cut(s) | 383 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 479 |
| BstC8I | GCNNGC | 2 cut(s) | 475, 502 |
| BstDEI | CTNAG | 2 cut(s) | 366, 405 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 491 |
| BstF5I | GGATG | 1 cut(s) | 222 |
| BstMAI | GTCTC | 1 cut(s) | 101 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 479 |
| BstNI | CCWGG | 2 cut(s) | 231, 266 |
| BstSCI | CCNGG | 2 cut(s) | 229, 264 |
| BstSFI | CTRYAG | 1 cut(s) | 471 |
| BstV1I | GCAGC | 3 cut(s) | 131, 172, 314 |
| BsuRI | GGCC | 1 cut(s) | 477 |
| BtrI | CACGTC | 1 cut(s) | 282 |
| BtsCI | GGATG | 1 cut(s) | 222 |
| BveI | ACCTGC | 1 cut(s) | 478 |
| Cac8I | GCNNGC | 2 cut(s) | 475, 502 |
| CciI | TCATGA | 1 cut(s) | 150 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 273, 308 |
| CviAII | CATG | 1 cut(s) | 151 |
| DdeI | CTNAG | 2 cut(s) | 366, 405 |
| Eco130I | CCWWGG | 1 cut(s) | 487 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 491 |
| EcoRI | GAATTC | 1 cut(s) | 386 |
| EcoRII | CCWGG | 2 cut(s) | 229, 264 |
| EcoT14I | CCWWGG | 1 cut(s) | 487 |
| ErhI | CCWWGG | 1 cut(s) | 487 |
| FaeI | CATG | 1 cut(s) | 154 |
| FaiI | YATR | 4 cut(s) | 77, 152, 167, 465 |
| FatI | CATG | 1 cut(s) | 150 |
| Fnu4HI | GCNGC | 3 cut(s) | 120, 186, 303 |
| FokI | GGATG | 1 cut(s) | 209 |
| Fsp4HI | GCNGC | 3 cut(s) | 120, 186, 303 |
| FspBI | CTAG | 1 cut(s) | 501 |
| GluI | GCNGC | 3 cut(s) | 120, 186, 303 |
| GsaI | CCCAGC | 1 cut(s) | 356 |
| GsuI | CTGGAG | 3 cut(s) | 126, 144, 248 |
| HaeIII | GGCC | 1 cut(s) | 477 |
| HapII | CCGG | 1 cut(s) | 251 |
| Hin1II | CATG | 1 cut(s) | 154 |
| HincII | GTYRAC | 1 cut(s) | 285 |
| HindII | GTYRAC | 1 cut(s) | 285 |
| HinfI | GANTC | 3 cut(s) | 83, 109, 209 |
| HpaII | CCGG | 1 cut(s) | 251 |
| HphI | GGTGA | 1 cut(s) | 395 |
| Hpy166II | GTNNAC | 1 cut(s) | 285 |
| Hpy188I | TCNGA | 2 cut(s) | 8, 208 |
| Hpy188III | TCNNGA | 2 cut(s) | 106, 151 |
| Hpy8I | GTNNAC | 1 cut(s) | 285 |
| HpyCH4III | ACNGT | 1 cut(s) | 383 |
| HpyCH4IV | ACGT | 1 cut(s) | 281 |
| HpyCH4V | TGCA | 3 cut(s) | 199, 424, 473 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 479 |
| HpyF3I | CTNAG | 2 cut(s) | 366, 405 |
| HpySE526I | ACGT | 1 cut(s) | 281 |
| Hsp92II | CATG | 1 cut(s) | 154 |
| LmnI | GCTCC | 5 cut(s) | 127, 145, 163, 281, 310 |
| Lsp1109I | GCAGC | 3 cut(s) | 131, 172, 314 |
| MaeI | CTAG | 1 cut(s) | 501 |
| MaeII | ACGT | 1 cut(s) | 281 |
| MaeIII | GTNAC | 1 cut(s) | 22 |
| MboII | GAAGA | 1 cut(s) | 427 |
| MhlI | GDGCHC | 1 cut(s) | 484 |
| MluCI | AATT | 3 cut(s) | 52, 386, 419 |
| MlyI | GAGTC | 2 cut(s) | 92, 103 |
| MmeI | TCCRAC | 2 cut(s) | 159, 225 |
| MnlI | CCTC | 7 cut(s) | 72, 175, 237, 318, 361, 370, 515 |
| MseI | TTAA | 1 cut(s) | 56 |
| MspA1I | CMGCKG | 2 cut(s) | 34, 352 |
| MspI | CCGG | 1 cut(s) | 251 |
| MspR9I | CCNGG | 2 cut(s) | 231, 266 |
| MvaI | CCWGG | 2 cut(s) | 231, 266 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 479 |
| NheI | GCTAGC | 1 cut(s) | 500 |
| NlaIII | CATG | 1 cut(s) | 154 |
| NlaIV | GGNNCC | 3 cut(s) | 30, 192, 249 |
| PagI | TCATGA | 1 cut(s) | 150 |
| PfeI | GAWTC | 1 cut(s) | 209 |
| PkrI | GCNGC | 3 cut(s) | 121, 187, 304 |
| PleI | GAGTC | 2 cut(s) | 91, 103 |
| PpsI | GAGTC | 2 cut(s) | 91, 103 |
| Psp6I | CCWGG | 2 cut(s) | 229, 264 |
| PspFI | CCCAGC | 1 cut(s) | 352 |
| PspGI | CCWGG | 2 cut(s) | 229, 264 |
| PspN4I | GGNNCC | 3 cut(s) | 30, 192, 249 |
| PstI | CTGCAG | 1 cut(s) | 475 |
| PvuII | CAGCTG | 1 cut(s) | 352 |
| SaqAI | TTAA | 1 cut(s) | 56 |
| SatI | GCNGC | 3 cut(s) | 120, 186, 303 |
| SbfI | CCTGCAGG | 1 cut(s) | 475 |
| SchI | GAGTC | 2 cut(s) | 92, 103 |
| ScrFI | CCNGG | 2 cut(s) | 231, 266 |
| SdaI | CCTGCAGG | 1 cut(s) | 475 |
| SduI | GDGCHC | 1 cut(s) | 484 |
| SetI | ASST | 9 cut(s) | 124, 142, 160, 278, 284, 307, 354, 372, 472 |
| SfcI | CTRYAG | 1 cut(s) | 471 |
| SmlI | CTYRAG | 1 cut(s) | 506 |
| SmoI | CTYRAG | 1 cut(s) | 506 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 475 |
| Sse9I | AATT | 3 cut(s) | 52, 386, 419 |
| SsiI | CCGC | 2 cut(s) | 32, 188 |
| SspMI | CTAG | 1 cut(s) | 501 |
| StyD4I | CCNGG | 2 cut(s) | 229, 264 |
| StyI | CCWWGG | 1 cut(s) | 487 |
| TaaI | ACNGT | 1 cut(s) | 383 |
| TaiI | ACGT | 1 cut(s) | 284 |
| TaqI | TCGA | 2 cut(s) | 105, 413 |
| TasI | AATT | 3 cut(s) | 52, 386, 419 |
| TfiI | GAWTC | 1 cut(s) | 209 |
| Tru1I | TTAA | 1 cut(s) | 56 |
| Tru9I | TTAA | 1 cut(s) | 56 |
| TseI | GCWGC | 3 cut(s) | 119, 185, 302 |
| TspDTI | ATGAA | 2 cut(s) | 78, 273 |
| TspGWI | ACGGA | 1 cut(s) | 149 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 491 |
| XapI | RAATTY | 2 cut(s) | 52, 386 |
| XspI | CTAG | 1 cut(s) | 501 |