Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 447 |
| AccI | GTMKAC | 1 cut(s) | 8 |
| AccII | CGCG | 1 cut(s) | 392 |
| AciI | CCGC | 2 cut(s) | 62, 77 |
| AcsI | RAATTY | 2 cut(s) | 481, 503 |
| AcyI | GRCGYC | 1 cut(s) | 92 |
| AfaI | GTAC | 1 cut(s) | 360 |
| AflII | CTTAAG | 1 cut(s) | 395 |
| AgsI | TTSAA | 1 cut(s) | 503 |
| AjnI | CCWGG | 2 cut(s) | 299, 400 |
| AluBI | AGCT | 3 cut(s) | 110, 271, 342 |
| AluI | AGCT | 3 cut(s) | 110, 271, 342 |
| Alw26I | GTCTC | 1 cut(s) | 298 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 300 |
| ApeKI | GCWGC | 1 cut(s) | 205 |
| ApoI | RAATTY | 2 cut(s) | 481, 503 |
| AspLEI | GCGC | 2 cut(s) | 394, 448 |
| AspS9I | GGNCC | 2 cut(s) | 426, 490 |
| AsuHPI | GGTGA | 1 cut(s) | 490 |
| AvaII | GGWCC | 2 cut(s) | 426, 490 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 314, 346 |
| BbvI | GCAGC | 1 cut(s) | 192 |
| BccI | CCATC | 3 cut(s) | 38, 161, 228 |
| BciT130I | CCWGG | 2 cut(s) | 301, 402 |
| BcoDI | GTCTC | 1 cut(s) | 298 |
| BfaI | CTAG | 2 cut(s) | 209, 272 |
| BfrI | CTTAAG | 1 cut(s) | 395 |
| BisI | GCNGC | 3 cut(s) | 62, 78, 206 |
| BlsI | GCNGC | 3 cut(s) | 63, 79, 207 |
| Bme1390I | CCNGG | 2 cut(s) | 301, 402 |
| Bme18I | GGWCC | 2 cut(s) | 426, 490 |
| BmgT120I | GGNCC | 2 cut(s) | 426, 490 |
| BmiI | GGNNCC | 2 cut(s) | 428, 491 |
| BmrFI | CCNGG | 2 cut(s) | 301, 402 |
| BmsI | GCATC | 3 cut(s) | 223, 343, 457 |
| BoxI | GACNNNNGTC | 1 cut(s) | 495 |
| BpmI | CTGGAG | 1 cut(s) | 322 |
| BpuEI | CTTGAG | 1 cut(s) | 453 |
| BsaBI | GATNNNNATC | 1 cut(s) | 171 |
| BsaHI | GRCGYC | 1 cut(s) | 92 |
| BsaJI | CCNNGG | 4 cut(s) | 29, 65, 299, 493 |
| Bse3DI | GCAATG | 1 cut(s) | 119 |
| Bse8I | GATNNNNATC | 1 cut(s) | 171 |
| BseBI | CCWGG | 2 cut(s) | 301, 402 |
| BseDI | CCNNGG | 4 cut(s) | 29, 65, 299, 493 |
| BseGI | GGATG | 2 cut(s) | 49, 172 |
| BseJI | GATNNNNATC | 1 cut(s) | 171 |
| BseMI | GCAATG | 1 cut(s) | 119 |
| BseMII | CTCAG | 1 cut(s) | 363 |
| BseXI | GCAGC | 1 cut(s) | 192 |
| BsgI | GTGCAG | 1 cut(s) | 72 |
| Bsh1236I | CGCG | 1 cut(s) | 392 |
| BslFI | GGGAC | 2 cut(s) | 282, 503 |
| BsmAI | GTCTC | 1 cut(s) | 298 |
| BsmFI | GGGAC | 2 cut(s) | 282, 503 |
| BsmI | GAATGC | 1 cut(s) | 469 |
| Bsp143I | GATC | 2 cut(s) | 291, 387 |
| Bsp19I | CCATGG | 1 cut(s) | 29 |
| BspACI | CCGC | 2 cut(s) | 62, 77 |
| BspCNI | CTCAG | 1 cut(s) | 362 |
| BspFNI | CGCG | 1 cut(s) | 392 |
| BspLI | GGNNCC | 2 cut(s) | 428, 491 |
| BspTI | CTTAAG | 1 cut(s) | 395 |
| BsrDI | GCAATG | 1 cut(s) | 119 |
| BssECI | CCNNGG | 4 cut(s) | 29, 65, 299, 493 |
| BssMI | GATC | 2 cut(s) | 291, 387 |
| BssNI | GRCGYC | 1 cut(s) | 92 |
| BssT1I | CCWWGG | 3 cut(s) | 29, 65, 493 |
| Bst2UI | CCWGG | 2 cut(s) | 301, 402 |
| Bst4CI | ACNGT | 1 cut(s) | 266 |
| BstACI | GRCGYC | 1 cut(s) | 92 |
| BstAFI | CTTAAG | 1 cut(s) | 395 |
| BstDEI | CTNAG | 1 cut(s) | 349 |
| BstDSI | CCRYGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 2 cut(s) | 49, 172 |
| BstFNI | CGCG | 1 cut(s) | 392 |
| BstHHI | GCGC | 2 cut(s) | 394, 448 |
| BstKTI | GATC | 2 cut(s) | 294, 390 |
| BstMAI | GTCTC | 1 cut(s) | 298 |
| BstMBI | GATC | 2 cut(s) | 291, 387 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 86, 211, 268 |
| BstNI | CCWGG | 2 cut(s) | 301, 402 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 495 |
| BstSCI | CCNGG | 2 cut(s) | 299, 400 |
| BstUI | CGCG | 1 cut(s) | 392 |
| BstV1I | GCAGC | 1 cut(s) | 192 |
| BtgI | CCRYGG | 1 cut(s) | 29 |
| BtsCI | GGATG | 2 cut(s) | 49, 172 |
| CaiI | CAGNNNCTG | 1 cut(s) | 300 |
| CfoI | GCGC | 2 cut(s) | 394, 448 |
| Cfr13I | GGNCC | 2 cut(s) | 426, 490 |
| CseI | GACGC | 1 cut(s) | 100 |
| Csp6I | GTAC | 1 cut(s) | 359 |
| CviAII | CATG | 2 cut(s) | 30, 134 |
| CviJI | RGCY | 6 cut(s) | 80, 110, 271, 327, 342, 400 |
| CviKI_1 | RGCY | 6 cut(s) | 80, 110, 271, 327, 342, 400 |
| CviQI | GTAC | 1 cut(s) | 359 |
| DdeI | CTNAG | 1 cut(s) | 349 |
| DpnI | GATC | 2 cut(s) | 293, 389 |
| DpnII | GATC | 2 cut(s) | 291, 387 |
| Eco130I | CCWWGG | 3 cut(s) | 29, 65, 493 |
| Eco47I | GGWCC | 2 cut(s) | 426, 490 |
| EcoO109I | RGGNCCY | 1 cut(s) | 490 |
| EcoRII | CCWGG | 2 cut(s) | 299, 400 |
| EcoT14I | CCWWGG | 3 cut(s) | 29, 65, 493 |
| ErhI | CCWWGG | 3 cut(s) | 29, 65, 493 |
| FaeI | CATG | 2 cut(s) | 33, 137 |
| FaiI | YATR | 8 cut(s) | 31, 75, 135, 201, 203, 311, 317, 419 |
| FaqI | GGGAC | 2 cut(s) | 282, 503 |
| FatI | CATG | 2 cut(s) | 29, 133 |
| FauNDI | CATATG | 1 cut(s) | 201 |
| FblI | GTMKAC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 3 cut(s) | 62, 78, 206 |
| FokI | GGATG | 2 cut(s) | 56, 179 |
| Fsp4HI | GCNGC | 3 cut(s) | 62, 78, 206 |
| FspBI | CTAG | 2 cut(s) | 209, 272 |
| FspI | TGCGCA | 1 cut(s) | 447 |
| GlaI | GCGC | 2 cut(s) | 393, 447 |
| GluI | GCNGC | 3 cut(s) | 62, 78, 206 |
| GsuI | CTGGAG | 1 cut(s) | 322 |
| HgaI | GACGC | 1 cut(s) | 100 |
| HhaI | GCGC | 2 cut(s) | 394, 448 |
| Hin1I | GRCGYC | 1 cut(s) | 92 |
| Hin1II | CATG | 2 cut(s) | 33, 137 |
| Hin6I | GCGC | 2 cut(s) | 392, 446 |
| HinP1I | GCGC | 2 cut(s) | 392, 446 |
| HinfI | GANTC | 1 cut(s) | 100 |
| HphI | GGTGA | 1 cut(s) | 490 |
| Hpy166II | GTNNAC | 1 cut(s) | 9 |
| Hpy188I | TCNGA | 3 cut(s) | 256, 352, 453 |
| Hpy188III | TCNNGA | 1 cut(s) | 500 |
| Hpy8I | GTNNAC | 1 cut(s) | 9 |
| Hpy99I | CGWCG | 1 cut(s) | 94 |
| HpyAV | CCTTC | 4 cut(s) | 131, 261, 316, 373 |
| HpyCH4III | ACNGT | 1 cut(s) | 266 |
| HpyCH4V | TGCA | 4 cut(s) | 53, 195, 262, 356 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 86, 211, 268 |
| HpyF3I | CTNAG | 1 cut(s) | 349 |
| Hsp92I | GRCGYC | 1 cut(s) | 92 |
| Hsp92II | CATG | 2 cut(s) | 33, 137 |
| HspAI | GCGC | 2 cut(s) | 392, 446 |
| Kzo9I | GATC | 2 cut(s) | 291, 387 |
| LpnPI | CCDG | 7 cut(s) | 138, 165, 286, 313, 387, 414, 456 |
| Lsp1109I | GCAGC | 1 cut(s) | 192 |
| LweI | GCATC | 3 cut(s) | 223, 343, 457 |
| MaeI | CTAG | 2 cut(s) | 209, 272 |
| MaeIII | GTNAC | 1 cut(s) | 82 |
| MalI | GATC | 2 cut(s) | 293, 389 |
| MboI | GATC | 2 cut(s) | 291, 387 |
| MboII | GAAGA | 2 cut(s) | 107, 466 |
| MluCI | AATT | 5 cut(s) | 150, 257, 433, 481, 503 |
| MmeI | TCCRAC | 2 cut(s) | 165, 404 |
| MnlI | CCTC | 1 cut(s) | 358 |
| MseI | TTAA | 2 cut(s) | 237, 396 |
| MspCI | CTTAAG | 1 cut(s) | 395 |
| MspR9I | CCNGG | 2 cut(s) | 301, 402 |
| Mva1269I | GAATGC | 1 cut(s) | 469 |
| MvaI | CCWGG | 2 cut(s) | 301, 402 |
| MvnI | CGCG | 1 cut(s) | 392 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 86, 211, 268 |
| NcoI | CCATGG | 1 cut(s) | 29 |
| NdeI | CATATG | 1 cut(s) | 201 |
| NdeII | GATC | 2 cut(s) | 291, 387 |
| NlaIII | CATG | 2 cut(s) | 33, 137 |
| NlaIV | GGNNCC | 2 cut(s) | 428, 491 |
| NmuCI | GTSAC | 1 cut(s) | 82 |
| NsbI | TGCGCA | 1 cut(s) | 447 |
| PctI | GAATGC | 1 cut(s) | 469 |
| PfeI | GAWTC | 1 cut(s) | 100 |
| PkrI | GCNGC | 3 cut(s) | 63, 79, 207 |
| PpuMI | RGGWCCY | 1 cut(s) | 490 |
| PshAI | GACNNNNGTC | 1 cut(s) | 495 |
| Psp5II | RGGWCCY | 1 cut(s) | 490 |
| Psp6I | CCWGG | 2 cut(s) | 299, 400 |
| PspGI | CCWGG | 2 cut(s) | 299, 400 |
| PspN4I | GGNNCC | 2 cut(s) | 428, 491 |
| PspPI | GGNCC | 2 cut(s) | 426, 490 |
| PspPPI | RGGWCCY | 1 cut(s) | 490 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 136, 168 |
| PstNI | CAGNNNCTG | 1 cut(s) | 300 |
| RsaI | GTAC | 1 cut(s) | 360 |
| RsaNI | GTAC | 1 cut(s) | 359 |
| SaqAI | TTAA | 2 cut(s) | 237, 396 |
| SatI | GCNGC | 3 cut(s) | 62, 78, 206 |
| Sau3AI | GATC | 2 cut(s) | 291, 387 |
| Sau96I | GGNCC | 2 cut(s) | 426, 490 |
| ScrFI | CCNGG | 2 cut(s) | 301, 402 |
| SetI | ASST | 7 cut(s) | 71, 112, 273, 316, 344, 416, 495 |
| SfaNI | GCATC | 3 cut(s) | 223, 343, 457 |
| SinI | GGWCC | 2 cut(s) | 426, 490 |
| SmlI | CTYRAG | 2 cut(s) | 395, 468 |
| SmoI | CTYRAG | 2 cut(s) | 395, 468 |
| Sse9I | AATT | 5 cut(s) | 150, 257, 433, 481, 503 |
| SsiI | CCGC | 2 cut(s) | 62, 77 |
| SspMI | CTAG | 2 cut(s) | 209, 272 |
| StyD4I | CCNGG | 2 cut(s) | 299, 400 |
| StyI | CCWWGG | 3 cut(s) | 29, 65, 493 |
| TaaI | ACNGT | 1 cut(s) | 266 |
| TasI | AATT | 5 cut(s) | 150, 257, 433, 481, 503 |
| TauI | GCSGC | 2 cut(s) | 64, 80 |
| TfiI | GAWTC | 1 cut(s) | 100 |
| Tru1I | TTAA | 2 cut(s) | 237, 396 |
| Tru9I | TTAA | 2 cut(s) | 237, 396 |
| TseFI | GTSAC | 1 cut(s) | 82 |
| TseI | GCWGC | 1 cut(s) | 205 |
| Tsp45I | GTSAC | 1 cut(s) | 82 |
| TspDTI | ATGAA | 1 cut(s) | 150 |
| Vha464I | CTTAAG | 1 cut(s) | 395 |
| VpaK11BI | GGWCC | 2 cut(s) | 426, 490 |
| XapI | RAATTY | 2 cut(s) | 481, 503 |
| XmiI | GTMKAC | 1 cut(s) | 8 |
| XspI | CTAG | 2 cut(s) | 209, 272 |