Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 440 |
| AciI | CCGC | 1 cut(s) | 181 |
| AclI | AACGTT | 1 cut(s) | 292 |
| AcsI | RAATTY | 2 cut(s) | 43, 583 |
| AdeI | CACNNNGTG | 1 cut(s) | 524 |
| AfaI | GTAC | 3 cut(s) | 69, 81, 392 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 416 |
| AgsI | TTSAA | 3 cut(s) | 468, 775, 805 |
| AhlI | ACTAGT | 1 cut(s) | 707 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 292, 324 |
| AluBI | AGCT | 4 cut(s) | 193, 197, 860, 866 |
| AluI | AGCT | 4 cut(s) | 193, 197, 860, 866 |
| Alw26I | GTCTC | 3 cut(s) | 345, 447, 452 |
| ApeKI | GCWGC | 2 cut(s) | 101, 197 |
| ApoI | RAATTY | 2 cut(s) | 43, 583 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 582, 614, 625, 657 |
| AseI | ATTAAT | 1 cut(s) | 617 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 153 |
| AspS9I | GGNCC | 1 cut(s) | 286 |
| AsuC2I | CCSGG | 1 cut(s) | 522 |
| AsuNHI | GCTAGC | 1 cut(s) | 193 |
| AvaII | GGWCC | 1 cut(s) | 286 |
| BbsI | GAAGAC | 1 cut(s) | 800 |
| BbvI | GCAGC | 2 cut(s) | 88, 184 |
| BclI | TGATCA | 1 cut(s) | 538 |
| BcnI | CCSGG | 1 cut(s) | 522 |
| BcoDI | GTCTC | 3 cut(s) | 345, 447, 452 |
| BcuI | ACTAGT | 1 cut(s) | 707 |
| BfaI | CTAG | 3 cut(s) | 38, 194, 708 |
| BfmI | CTRYAG | 3 cut(s) | 102, 198, 836 |
| BglII | AGATCT | 1 cut(s) | 749 |
| BisI | GCNGC | 3 cut(s) | 102, 182, 198 |
| BlsI | GCNGC | 3 cut(s) | 103, 183, 199 |
| Bme1390I | CCNGG | 1 cut(s) | 522 |
| Bme18I | GGWCC | 1 cut(s) | 286 |
| BmgT120I | GGNCC | 1 cut(s) | 286 |
| BmrFI | CCNGG | 1 cut(s) | 522 |
| BmsI | GCATC | 6 cut(s) | 42, 43, 196, 307, 387, 768 |
| BmtI | GCTAGC | 1 cut(s) | 197 |
| BpiI | GAAGAC | 1 cut(s) | 800 |
| BpuEI | CTTGAG | 1 cut(s) | 846 |
| BpuMI | CCSGG | 1 cut(s) | 522 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 342, 372 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 416 |
| Bse3DI | GCAATG | 3 cut(s) | 276, 510, 564 |
| BseGI | GGATG | 2 cut(s) | 211, 286 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BseMI | GCAATG | 3 cut(s) | 276, 510, 564 |
| BseMII | CTCAG | 1 cut(s) | 488 |
| BseXI | GCAGC | 2 cut(s) | 88, 184 |
| BsiSI | CCGG | 1 cut(s) | 521 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BsmAI | GTCTC | 3 cut(s) | 345, 447, 452 |
| BsmBI | CGTCTC | 2 cut(s) | 345, 447 |
| Bsp1407I | TGTACA | 1 cut(s) | 67 |
| Bsp143I | GATC | 4 cut(s) | 25, 56, 538, 749 |
| BspACI | CCGC | 1 cut(s) | 181 |
| BspCNI | CTCAG | 1 cut(s) | 489 |
| BspMAI | CTGCAG | 2 cut(s) | 106, 202 |
| BspOI | GCTAGC | 1 cut(s) | 197 |
| BsrDI | GCAATG | 3 cut(s) | 276, 510, 564 |
| BsrGI | TGTACA | 1 cut(s) | 67 |
| BssMI | GATC | 4 cut(s) | 25, 56, 538, 749 |
| Bst4CI | ACNGT | 1 cut(s) | 19 |
| Bst6I | CTCTTC | 2 cut(s) | 240, 708 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 206 |
| BstAUI | TGTACA | 1 cut(s) | 67 |
| BstC8I | GCNNGC | 1 cut(s) | 195 |
| BstDEI | CTNAG | 2 cut(s) | 158, 497 |
| BstF5I | GGATG | 2 cut(s) | 211, 286 |
| BstKTI | GATC | 4 cut(s) | 28, 59, 541, 752 |
| BstMAI | GTCTC | 3 cut(s) | 345, 447, 452 |
| BstMBI | GATC | 4 cut(s) | 25, 56, 538, 749 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 61, 107, 190, 206, 493 |
| BstNSI | RCATGY | 1 cut(s) | 241 |
| BstSCI | CCNGG | 1 cut(s) | 520 |
| BstSFI | CTRYAG | 3 cut(s) | 102, 198, 836 |
| BstV1I | GCAGC | 2 cut(s) | 88, 184 |
| BstV2I | GAAGAC | 1 cut(s) | 800 |
| BstX2I | RGATCY | 1 cut(s) | 749 |
| BstYI | RGATCY | 1 cut(s) | 749 |
| BtgZI | GCGATG | 1 cut(s) | 578 |
| BtsCI | GGATG | 2 cut(s) | 211, 286 |
| BtsI | GCAGTG | 1 cut(s) | 111 |
| BtsIMutI | CAGTG | 1 cut(s) | 111 |
| Cac8I | GCNNGC | 1 cut(s) | 195 |
| Cfr13I | GGNCC | 1 cut(s) | 286 |
| Csp6I | GTAC | 3 cut(s) | 68, 80, 391 |
| CviAII | CATG | 5 cut(s) | 119, 238, 492, 553, 702 |
| CviJI | RGCY | 9 cut(s) | 110, 193, 197, 496, 551, 563, 833, 860, 866 |
| CviKI_1 | RGCY | 9 cut(s) | 110, 193, 197, 496, 551, 563, 833, 860, 866 |
| CviQI | GTAC | 3 cut(s) | 68, 80, 391 |
| DdeI | CTNAG | 2 cut(s) | 158, 497 |
| DpnI | GATC | 4 cut(s) | 27, 58, 540, 751 |
| DpnII | GATC | 4 cut(s) | 25, 56, 538, 749 |
| DraIII | CACNNNGTG | 1 cut(s) | 524 |
| Eam1104I | CTCTTC | 2 cut(s) | 240, 708 |
| EarI | CTCTTC | 2 cut(s) | 240, 708 |
| Eco32I | GATATC | 1 cut(s) | 128 |
| Eco47I | GGWCC | 1 cut(s) | 286 |
| EcoRI | GAATTC | 1 cut(s) | 43 |
| EcoRV | GATATC | 1 cut(s) | 128 |
| EcoT22I | ATGCAT | 1 cut(s) | 692 |
| Esp3I | CGTCTC | 2 cut(s) | 345, 447 |
| FaeI | CATG | 5 cut(s) | 122, 241, 495, 556, 705 |
| FatI | CATG | 5 cut(s) | 118, 237, 491, 552, 701 |
| FbaI | TGATCA | 1 cut(s) | 538 |
| FblI | GTMKAC | 1 cut(s) | 440 |
| Fnu4HI | GCNGC | 3 cut(s) | 102, 182, 198 |
| FokI | GGATG | 2 cut(s) | 218, 293 |
| Fsp4HI | GCNGC | 3 cut(s) | 102, 182, 198 |
| FspBI | CTAG | 3 cut(s) | 38, 194, 708 |
| GluI | GCNGC | 3 cut(s) | 102, 182, 198 |
| HapII | CCGG | 1 cut(s) | 521 |
| Hin1II | CATG | 5 cut(s) | 122, 241, 495, 556, 705 |
| HinfI | GANTC | 1 cut(s) | 250 |
| HpaII | CCGG | 1 cut(s) | 521 |
| Hpy166II | GTNNAC | 2 cut(s) | 424, 441 |
| Hpy188I | TCNGA | 2 cut(s) | 754, 875 |
| Hpy188III | TCNNGA | 4 cut(s) | 38, 329, 542, 588 |
| Hpy8I | GTNNAC | 2 cut(s) | 424, 441 |
| Hpy99I | CGWCG | 1 cut(s) | 26 |
| HpyCH4III | ACNGT | 1 cut(s) | 19 |
| HpyCH4IV | ACGT | 2 cut(s) | 292, 354 |
| HpyCH4V | TGCA | 9 cut(s) | 104, 200, 235, 241, 269, 320, 487, 569, 690 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 61, 107, 190, 206, 493 |
| HpyF3I | CTNAG | 2 cut(s) | 158, 497 |
| HpySE526I | ACGT | 2 cut(s) | 292, 354 |
| Hsp92II | CATG | 5 cut(s) | 122, 241, 495, 556, 705 |
| Ksp22I | TGATCA | 1 cut(s) | 538 |
| Kzo9I | GATC | 4 cut(s) | 25, 56, 538, 749 |
| LmnI | GCTCC | 2 cut(s) | 190, 412 |
| LpnPI | CCDG | 3 cut(s) | 186, 534, 824 |
| Lsp1109I | GCAGC | 2 cut(s) | 88, 184 |
| LweI | GCATC | 6 cut(s) | 42, 43, 196, 307, 387, 768 |
| MaeI | CTAG | 3 cut(s) | 38, 194, 708 |
| MaeII | ACGT | 2 cut(s) | 292, 354 |
| MalI | GATC | 4 cut(s) | 27, 58, 540, 751 |
| MboI | GATC | 4 cut(s) | 25, 56, 538, 749 |
| MboII | GAAGA | 6 cut(s) | 257, 344, 674, 725, 763, 805 |
| MflI | RGATCY | 1 cut(s) | 749 |
| MluCI | AATT | 5 cut(s) | 43, 256, 583, 697, 763 |
| MmeI | TCCRAC | 2 cut(s) | 55, 796 |
| MnlI | CCTC | 4 cut(s) | 277, 503, 565, 639 |
| Mph1103I | ATGCAT | 1 cut(s) | 692 |
| MroXI | GAANNNNTTC | 1 cut(s) | 153 |
| MseI | TTAA | 4 cut(s) | 11, 482, 533, 617 |
| MspI | CCGG | 1 cut(s) | 521 |
| MspR9I | CCNGG | 1 cut(s) | 522 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 61, 107, 190, 206, 493 |
| NciI | CCSGG | 1 cut(s) | 522 |
| NdeII | GATC | 4 cut(s) | 25, 56, 538, 749 |
| NheI | GCTAGC | 1 cut(s) | 193 |
| NlaIII | CATG | 5 cut(s) | 122, 241, 495, 556, 705 |
| NsiI | ATGCAT | 1 cut(s) | 692 |
| NspI | RCATGY | 1 cut(s) | 241 |
| PdmI | GAANNNNTTC | 1 cut(s) | 153 |
| PfeI | GAWTC | 1 cut(s) | 250 |
| PkrI | GCNGC | 3 cut(s) | 103, 183, 199 |
| PshBI | ATTAAT | 1 cut(s) | 617 |
| Psp1406I | AACGTT | 1 cut(s) | 292 |
| PspPI | GGNCC | 1 cut(s) | 286 |
| PstI | CTGCAG | 2 cut(s) | 106, 202 |
| PsuI | RGATCY | 1 cut(s) | 749 |
| RsaI | GTAC | 3 cut(s) | 69, 81, 392 |
| RsaNI | GTAC | 3 cut(s) | 68, 80, 391 |
| SaqAI | TTAA | 4 cut(s) | 11, 482, 533, 617 |
| SatI | GCNGC | 3 cut(s) | 102, 182, 198 |
| Sau3AI | GATC | 4 cut(s) | 25, 56, 538, 749 |
| Sau96I | GGNCC | 1 cut(s) | 286 |
| ScrFI | CCNGG | 1 cut(s) | 522 |
| SfaNI | GCATC | 6 cut(s) | 42, 43, 196, 307, 387, 768 |
| SfcI | CTRYAG | 3 cut(s) | 102, 198, 836 |
| SinI | GGWCC | 1 cut(s) | 286 |
| SmlI | CTYRAG | 1 cut(s) | 861 |
| SmoI | CTYRAG | 1 cut(s) | 861 |
| SpeI | ACTAGT | 1 cut(s) | 707 |
| Sse9I | AATT | 5 cut(s) | 43, 256, 583, 697, 763 |
| SsiI | CCGC | 1 cut(s) | 181 |
| SspI | AATATT | 1 cut(s) | 136 |
| SspMI | CTAG | 3 cut(s) | 38, 194, 708 |
| StyD4I | CCNGG | 1 cut(s) | 520 |
| TaaI | ACNGT | 1 cut(s) | 19 |
| TaiI | ACGT | 2 cut(s) | 295, 357 |
| TaqI | TCGA | 2 cut(s) | 24, 589 |
| TasI | AATT | 5 cut(s) | 43, 256, 583, 697, 763 |
| TatI | WGTACW | 3 cut(s) | 67, 79, 390 |
| TauI | GCSGC | 1 cut(s) | 184 |
| TfiI | GAWTC | 1 cut(s) | 250 |
| Tru1I | TTAA | 4 cut(s) | 11, 482, 533, 617 |
| Tru9I | TTAA | 4 cut(s) | 11, 482, 533, 617 |
| TscAI | CASTG | 1 cut(s) | 111 |
| TseI | GCWGC | 2 cut(s) | 101, 197 |
| TspDTI | ATGAA | 5 cut(s) | 36, 569, 635, 675, 690 |
| TspGWI | ACGGA | 1 cut(s) | 399 |
| TspRI | CASTG | 1 cut(s) | 111 |
| VpaK11BI | GGWCC | 1 cut(s) | 286 |
| VspI | ATTAAT | 1 cut(s) | 617 |
| XapI | RAATTY | 2 cut(s) | 43, 583 |
| XbaI | TCTAGA | 1 cut(s) | 37 |
| XceI | RCATGY | 1 cut(s) | 241 |
| XmiI | GTMKAC | 1 cut(s) | 440 |
| XmnI | GAANNNNTTC | 1 cut(s) | 153 |
| XspI | CTAG | 3 cut(s) | 38, 194, 708 |
| Zsp2I | ATGCAT | 1 cut(s) | 692 |