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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 172 |
| AcsI | RAATTY | 2 cut(s) | 37, 142 |
| AgsI | TTSAA | 1 cut(s) | 34 |
| AluBI | AGCT | 1 cut(s) | 56 |
| AluI | AGCT | 1 cut(s) | 56 |
| ApoI | RAATTY | 2 cut(s) | 37, 142 |
| BccI | CCATC | 1 cut(s) | 169 |
| BclI | TGATCA | 1 cut(s) | 181 |
| BfaI | CTAG | 1 cut(s) | 200 |
| BmiI | GGNNCC | 2 cut(s) | 69, 205 |
| Bse1I | ACTGG | 1 cut(s) | 207 |
| BseGI | GGATG | 3 cut(s) | 160, 180, 184 |
| BseNI | ACTGG | 1 cut(s) | 207 |
| Bsp143I | GATC | 1 cut(s) | 181 |
| BspACI | CCGC | 1 cut(s) | 172 |
| BspLI | GGNNCC | 2 cut(s) | 69, 205 |
| BsrI | ACTGG | 1 cut(s) | 207 |
| BssMI | GATC | 1 cut(s) | 181 |
| BstC8I | GCNNGC | 1 cut(s) | 58 |
| BstF5I | GGATG | 3 cut(s) | 160, 180, 184 |
| BstKTI | GATC | 1 cut(s) | 184 |
| BstMBI | GATC | 1 cut(s) | 181 |
| BtsCI | GGATG | 3 cut(s) | 160, 180, 184 |
| Cac8I | GCNNGC | 1 cut(s) | 58 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 29 |
| CviJI | RGCY | 1 cut(s) | 56 |
| CviKI_1 | RGCY | 1 cut(s) | 56 |
| DpnI | GATC | 1 cut(s) | 183 |
| DpnII | GATC | 1 cut(s) | 181 |
| DraI | TTTAAA | 1 cut(s) | 21 |
| FbaI | TGATCA | 1 cut(s) | 181 |
| FokI | GGATG | 3 cut(s) | 167, 187, 191 |
| FspBI | CTAG | 1 cut(s) | 200 |
| HindIII | AAGCTT | 1 cut(s) | 54 |
| Hpy188I | TCNGA | 1 cut(s) | 83 |
| Hpy188III | TCNNGA | 1 cut(s) | 185 |
| Ksp22I | TGATCA | 1 cut(s) | 181 |
| Kzo9I | GATC | 1 cut(s) | 181 |
| LpnPI | CCDG | 4 cut(s) | 24, 124, 136, 150 |
| MaeI | CTAG | 1 cut(s) | 200 |
| MalI | GATC | 1 cut(s) | 183 |
| MboI | GATC | 1 cut(s) | 181 |
| MluCI | AATT | 4 cut(s) | 6, 26, 37, 142 |
| MseI | TTAA | 1 cut(s) | 20 |
| MspA1I | CMGCKG | 1 cut(s) | 172 |
| NdeII | GATC | 1 cut(s) | 181 |
| NlaIV | GGNNCC | 2 cut(s) | 69, 205 |
| PspN4I | GGNNCC | 2 cut(s) | 69, 205 |
| SaqAI | TTAA | 1 cut(s) | 20 |
| Sau3AI | GATC | 1 cut(s) | 181 |
| SetI | ASST | 3 cut(s) | 58, 169, 205 |
| SgeI | CNNG | 9 cut(s) | 23, 69, 84, 123, 137, 163, 177, 197, 212 |
| Sse9I | AATT | 4 cut(s) | 6, 26, 37, 142 |
| SsiI | CCGC | 1 cut(s) | 172 |
| SspMI | CTAG | 1 cut(s) | 200 |
| TasI | AATT | 4 cut(s) | 6, 26, 37, 142 |
| Tru1I | TTAA | 1 cut(s) | 20 |
| Tru9I | TTAA | 1 cut(s) | 20 |
| TspDTI | ATGAA | 1 cut(s) | 39 |
| XapI | RAATTY | 2 cut(s) | 37, 142 |
| XspI | CTAG | 1 cut(s) | 200 |