Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 3 cut(s) | 913, 940, 2011 |
| Acc65I | GGTACC | 1 cut(s) | 1803 |
| AccB1I | GGYRCC | 1 cut(s) | 1803 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1355, 1421 |
| AciI | CCGC | 2 cut(s) | 46, 1988 |
| AclWI | GGATC | 4 cut(s) | 516, 525, 530, 1822 |
| AcsI | RAATTY | 3 cut(s) | 647, 1559, 2012 |
| AcuI | CTGAAG | 2 cut(s) | 791, 1208 |
| AcyI | GRCGYC | 1 cut(s) | 669 |
| AfaI | GTAC | 4 cut(s) | 335, 1401, 1753, 1805 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 629, 1355, 1421 |
| AflIII | ACRYGT | 1 cut(s) | 1967 |
| AjiI | CACGTC | 1 cut(s) | 1949 |
| AjnI | CCWGG | 3 cut(s) | 542, 1354, 1579 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 359, 391 |
| Alw21I | GWGCWC | 4 cut(s) | 197, 504, 681, 697 |
| Alw26I | GTCTC | 3 cut(s) | 137, 571, 2038 |
| Alw44I | GTGCAC | 1 cut(s) | 193 |
| AlwI | GGATC | 4 cut(s) | 516, 525, 530, 1822 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1355, 1421, 1510 |
| AoxI | GGCC | 4 cut(s) | 50, 1031, 1119, 1878 |
| ApaLI | GTGCAC | 1 cut(s) | 193 |
| ApeKI | GCWGC | 6 cut(s) | 1055, 1142, 1343, 1903, 1914, 2006 |
| ApoI | RAATTY | 3 cut(s) | 647, 1559, 2012 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 563, 595 |
| AseI | ATTAAT | 1 cut(s) | 531 |
| Asp718I | GGTACC | 1 cut(s) | 1803 |
| AspLEI | GCGC | 1 cut(s) | 1078 |
| AspS9I | GGNCC | 4 cut(s) | 110, 579, 754, 1120 |
| AsuC2I | CCSGG | 2 cut(s) | 87, 1123 |
| AsuHPI | GGTGA | 4 cut(s) | 1595, 1696, 1843, 1877 |
| AsuII | TTCGAA | 1 cut(s) | 538 |
| AvaII | GGWCC | 3 cut(s) | 110, 579, 754 |
| BaeGI | GKGCMC | 1 cut(s) | 197 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1743, 1776 |
| BanI | GGYRCC | 1 cut(s) | 1803 |
| BanII | GRGCYC | 1 cut(s) | 504 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 317, 349 |
| BbsI | GAAGAC | 2 cut(s) | 972, 1048 |
| Bbv12I | GWGCWC | 4 cut(s) | 197, 504, 681, 697 |
| BbvI | GCAGC | 6 cut(s) | 1067, 1154, 1330, 1901, 1915, 2018 |
| BceAI | ACGGC | 1 cut(s) | 1215 |
| BciT130I | CCWGG | 3 cut(s) | 544, 1356, 1581 |
| BciVI | GTATCC | 1 cut(s) | 1648 |
| BcnI | CCSGG | 2 cut(s) | 87, 1123 |
| BcoDI | GTCTC | 3 cut(s) | 137, 571, 2038 |
| BfaI | CTAG | 3 cut(s) | 1046, 1146, 1430 |
| BfmI | CTRYAG | 4 cut(s) | 790, 906, 1851, 2004 |
| BfuAI | ACCTGC | 3 cut(s) | 913, 940, 2011 |
| BfuI | GTATCC | 1 cut(s) | 1648 |
| BglII | AGATCT | 1 cut(s) | 829 |
| BisI | GCNGC | 6 cut(s) | 1056, 1143, 1344, 1904, 1915, 2007 |
| BlsI | GCNGC | 6 cut(s) | 1057, 1144, 1345, 1905, 1916, 2008 |
| Bme1390I | CCNGG | 5 cut(s) | 87, 544, 1123, 1356, 1581 |
| Bme18I | GGWCC | 3 cut(s) | 110, 579, 754 |
| BmgBI | CACGTC | 1 cut(s) | 1949 |
| BmgT120I | GGNCC | 4 cut(s) | 110, 579, 754, 1120 |
| BmiI | GGNNCC | 4 cut(s) | 68, 84, 111, 1805 |
| BmrFI | CCNGG | 5 cut(s) | 87, 544, 1123, 1356, 1581 |
| BmrI | ACTGGG | 1 cut(s) | 116 |
| BmsI | GCATC | 8 cut(s) | 284, 465, 493, 628, 1129, 1308, 1597, 1921 |
| BmuI | ACTGGG | 1 cut(s) | 116 |
| BpiI | GAAGAC | 2 cut(s) | 972, 1048 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 868, 900 |
| BpmI | CTGGAG | 3 cut(s) | 1332, 1563, 1755 |
| Bpu14I | TTCGAA | 1 cut(s) | 538 |
| BpuEI | CTTGAG | 2 cut(s) | 179, 719 |
| BpuMI | CCSGG | 2 cut(s) | 87, 1123 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1691 |
| BsaHI | GRCGYC | 1 cut(s) | 669 |
| BsaJI | CCNNGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 629, 1355, 1421 |
| Bse1I | ACTGG | 6 cut(s) | 111, 304, 368, 757, 1349, 1608 |
| Bse3DI | GCAATG | 1 cut(s) | 1653 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1691 |
| BseBI | CCWGG | 3 cut(s) | 544, 1356, 1581 |
| BseDI | CCNNGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| BseJI | GATNNNNATC | 1 cut(s) | 1691 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 629, 1355, 1421 |
| BseMI | GCAATG | 1 cut(s) | 1653 |
| BseMII | CTCAG | 3 cut(s) | 850, 1299, 1629 |
| BseNI | ACTGG | 6 cut(s) | 111, 304, 368, 757, 1349, 1608 |
| BseSI | GKGCMC | 1 cut(s) | 197 |
| BseXI | GCAGC | 6 cut(s) | 1067, 1154, 1330, 1901, 1915, 2018 |
| BsgI | GTGCAG | 2 cut(s) | 171, 855 |
| BshFI | GGCC | 4 cut(s) | 52, 1033, 1121, 1880 |
| BshNI | GGYRCC | 1 cut(s) | 1803 |
| BsiHKAI | GWGCWC | 4 cut(s) | 197, 504, 681, 697 |
| BsiSI | CCGG | 2 cut(s) | 87, 1123 |
| BslFI | GGGAC | 1 cut(s) | 1928 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 629, 1355, 1421 |
| BsmAI | GTCTC | 3 cut(s) | 137, 571, 2038 |
| BsmBI | CGTCTC | 1 cut(s) | 137 |
| BsmFI | GGGAC | 1 cut(s) | 1928 |
| BsmI | GAATGC | 2 cut(s) | 928, 1078 |
| BsnI | GGCC | 4 cut(s) | 52, 1033, 1121, 1880 |
| Bsp119I | TTCGAA | 1 cut(s) | 538 |
| Bsp1286I | GDGCHC | 4 cut(s) | 197, 504, 681, 697 |
| Bsp143I | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| Bsp19I | CCATGG | 3 cut(s) | 582, 601, 655 |
| BspACI | CCGC | 2 cut(s) | 46, 1988 |
| BspANI | GGCC | 4 cut(s) | 52, 1033, 1121, 1880 |
| BspCNI | CTCAG | 3 cut(s) | 849, 1300, 1630 |
| BspHI | TCATGA | 2 cut(s) | 208, 1273 |
| BspLI | GGNNCC | 4 cut(s) | 68, 84, 111, 1805 |
| BspMAI | CTGCAG | 2 cut(s) | 910, 2008 |
| BspMI | ACCTGC | 3 cut(s) | 913, 940, 2011 |
| BspPI | GGATC | 4 cut(s) | 516, 525, 530, 1822 |
| BspQI | GCTCTTC | 1 cut(s) | 759 |
| BspT104I | TTCGAA | 1 cut(s) | 538 |
| BspT107I | GGYRCC | 1 cut(s) | 1803 |
| BsrDI | GCAATG | 1 cut(s) | 1653 |
| BsrI | ACTGG | 6 cut(s) | 111, 304, 368, 757, 1349, 1608 |
| BssECI | CCNNGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| BssMI | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| BssNI | GRCGYC | 1 cut(s) | 669 |
| BssT1I | CCWWGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| Bst2UI | CCWGG | 3 cut(s) | 544, 1356, 1581 |
| Bst6I | CTCTTC | 5 cut(s) | 32, 204, 243, 320, 759 |
| BstACI | GRCGYC | 1 cut(s) | 669 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 905, 932, 1132 |
| BstBI | TTCGAA | 1 cut(s) | 538 |
| BstC8I | GCNNGC | 5 cut(s) | 31, 182, 596, 862, 1035 |
| BstDEI | CTNAG | 6 cut(s) | 480, 707, 836, 1308, 1461, 1638 |
| BstDSI | CCRYGG | 3 cut(s) | 582, 601, 655 |
| BstHHI | GCGC | 1 cut(s) | 1078 |
| BstKTI | GATC | 9 cut(s) | 286, 511, 520, 525, 732, 832, 957, 1279, 1817 |
| BstMAI | GTCTC | 3 cut(s) | 137, 571, 2038 |
| BstMBI | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 288, 660, 905, 932, 1132, 1445, 1643 |
| BstNI | CCWGG | 3 cut(s) | 544, 1356, 1581 |
| BstNSI | RCATGY | 3 cut(s) | 184, 1400, 1971 |
| BstSCI | CCNGG | 5 cut(s) | 85, 542, 1121, 1354, 1579 |
| BstSFI | CTRYAG | 4 cut(s) | 790, 906, 1851, 2004 |
| BstSLI | GKGCMC | 1 cut(s) | 197 |
| BstV1I | GCAGC | 6 cut(s) | 1067, 1154, 1330, 1901, 1915, 2018 |
| BstV2I | GAAGAC | 2 cut(s) | 972, 1048 |
| BstX2I | RGATCY | 3 cut(s) | 508, 522, 829 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 77 |
| BstYI | RGATCY | 3 cut(s) | 508, 522, 829 |
| BsuI | GTATCC | 1 cut(s) | 1648 |
| BsuRI | GGCC | 4 cut(s) | 52, 1033, 1121, 1880 |
| BtgI | CCRYGG | 3 cut(s) | 582, 601, 655 |
| BtrI | CACGTC | 1 cut(s) | 1949 |
| BtsI | GCAGTG | 2 cut(s) | 286, 2025 |
| BveI | ACCTGC | 3 cut(s) | 913, 940, 2011 |
| Cac8I | GCNNGC | 5 cut(s) | 31, 182, 596, 862, 1035 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1355, 1421, 1510 |
| CciI | TCATGA | 2 cut(s) | 208, 1273 |
| CfoI | GCGC | 1 cut(s) | 1078 |
| Cfr13I | GGNCC | 4 cut(s) | 110, 579, 754, 1120 |
| CseI | GACGC | 1 cut(s) | 658 |
| Csp6I | GTAC | 4 cut(s) | 334, 1400, 1752, 1804 |
| CviQI | GTAC | 4 cut(s) | 334, 1400, 1752, 1804 |
| DdeI | CTNAG | 6 cut(s) | 480, 707, 836, 1308, 1461, 1638 |
| DpnI | GATC | 9 cut(s) | 285, 510, 519, 524, 731, 831, 956, 1278, 1816 |
| DpnII | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| Eam1104I | CTCTTC | 5 cut(s) | 32, 204, 243, 320, 759 |
| EarI | CTCTTC | 5 cut(s) | 32, 204, 243, 320, 759 |
| EciI | GGCGGA | 1 cut(s) | 1977 |
| Ecl136II | GAGCTC | 1 cut(s) | 502 |
| Eco130I | CCWWGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| Eco24I | GRGCYC | 1 cut(s) | 504 |
| Eco32I | GATATC | 1 cut(s) | 1412 |
| Eco47I | GGWCC | 3 cut(s) | 110, 579, 754 |
| Eco53kI | GAGCTC | 1 cut(s) | 502 |
| Eco57I | CTGAAG | 2 cut(s) | 791, 1208 |
| EcoICRI | GAGCTC | 1 cut(s) | 502 |
| EcoRI | GAATTC | 1 cut(s) | 1559 |
| EcoRII | CCWGG | 3 cut(s) | 542, 1354, 1579 |
| EcoRV | GATATC | 1 cut(s) | 1412 |
| EcoT14I | CCWWGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| EcoT22I | ATGCAT | 1 cut(s) | 458 |
| EcoT38I | GRGCYC | 1 cut(s) | 504 |
| ErhI | CCWWGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| Esp3I | CGTCTC | 1 cut(s) | 137 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 848, 880, 1305, 1337 |
| FaqI | GGGAC | 1 cut(s) | 1928 |
| Fnu4HI | GCNGC | 6 cut(s) | 1056, 1143, 1344, 1904, 1915, 2007 |
| FriOI | GRGCYC | 1 cut(s) | 504 |
| Fsp4HI | GCNGC | 6 cut(s) | 1056, 1143, 1344, 1904, 1915, 2007 |
| FspBI | CTAG | 3 cut(s) | 1046, 1146, 1430 |
| GlaI | GCGC | 1 cut(s) | 1077 |
| GluI | GCNGC | 6 cut(s) | 1056, 1143, 1344, 1904, 1915, 2007 |
| GsuI | CTGGAG | 3 cut(s) | 1332, 1563, 1755 |
| HaeIII | GGCC | 4 cut(s) | 52, 1033, 1121, 1880 |
| HapII | CCGG | 2 cut(s) | 87, 1123 |
| HgaI | GACGC | 1 cut(s) | 658 |
| HhaI | GCGC | 1 cut(s) | 1078 |
| Hin1I | GRCGYC | 1 cut(s) | 669 |
| Hin6I | GCGC | 1 cut(s) | 1076 |
| HinP1I | GCGC | 1 cut(s) | 1076 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 7 cut(s) | 13, 703, 944, 1216, 1240, 1418, 1709 |
| HpaII | CCGG | 2 cut(s) | 87, 1123 |
| HphI | GGTGA | 4 cut(s) | 1595, 1696, 1843, 1877 |
| Hpy166II | GTNNAC | 2 cut(s) | 195, 1369 |
| Hpy188I | TCNGA | 8 cut(s) | 268, 522, 839, 966, 1254, 1303, 1714, 1819 |
| Hpy188III | TCNNGA | 9 cut(s) | 209, 259, 952, 1274, 1415, 1422, 1564, 1734, 2036 |
| Hpy8I | GTNNAC | 2 cut(s) | 195, 1369 |
| HpyAV | CCTTC | 4 cut(s) | 286, 464, 964, 1090 |
| HpyCH4IV | ACGT | 3 cut(s) | 255, 1948, 1974 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 288, 660, 905, 932, 1132, 1445, 1643 |
| HpyF3I | CTNAG | 6 cut(s) | 480, 707, 836, 1308, 1461, 1638 |
| HpySE526I | ACGT | 3 cut(s) | 255, 1948, 1974 |
| Hsp92I | GRCGYC | 1 cut(s) | 669 |
| HspAI | GCGC | 1 cut(s) | 1076 |
| KpnI | GGTACC | 1 cut(s) | 1807 |
| Kzo9I | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| LguI | GCTCTTC | 1 cut(s) | 759 |
| LmnI | GCTCC | 3 cut(s) | 603, 1351, 1736 |
| Lsp1109I | GCAGC | 6 cut(s) | 1067, 1154, 1330, 1901, 1915, 2018 |
| LweI | GCATC | 8 cut(s) | 284, 465, 493, 628, 1129, 1308, 1597, 1921 |
| MaeI | CTAG | 3 cut(s) | 1046, 1146, 1430 |
| MaeII | ACGT | 3 cut(s) | 255, 1948, 1974 |
| MaeIII | GTNAC | 9 cut(s) | 164, 308, 641, 746, 785, 817, 1191, 1506, 1970 |
| MalI | GATC | 9 cut(s) | 285, 510, 519, 524, 731, 831, 956, 1278, 1816 |
| MboI | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| MflI | RGATCY | 3 cut(s) | 508, 522, 829 |
| MhlI | GDGCHC | 4 cut(s) | 197, 504, 681, 697 |
| MluCI | AATT | 9 cut(s) | 96, 345, 423, 647, 777, 1359, 1554, 1559, 2012 |
| MmeI | TCCRAC | 6 cut(s) | 284, 291, 1155, 1268, 1597, 1741 |
| Mph1103I | ATGCAT | 1 cut(s) | 458 |
| MseI | TTAA | 4 cut(s) | 422, 531, 716, 1281 |
| MslI | CAYNNNNRTG | 4 cut(s) | 432, 489, 684, 1826 |
| MspA1I | CMGCKG | 1 cut(s) | 1343 |
| MspI | CCGG | 2 cut(s) | 87, 1123 |
| MspR9I | CCNGG | 5 cut(s) | 87, 544, 1123, 1356, 1581 |
| Mva1269I | GAATGC | 2 cut(s) | 928, 1078 |
| MvaI | CCWGG | 3 cut(s) | 544, 1356, 1581 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 288, 660, 905, 932, 1132, 1445, 1643 |
| NciI | CCSGG | 2 cut(s) | 87, 1123 |
| NcoI | CCATGG | 3 cut(s) | 582, 601, 655 |
| NdeII | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| NlaIV | GGNNCC | 4 cut(s) | 68, 84, 111, 1805 |
| NmuCI | GTSAC | 2 cut(s) | 746, 1191 |
| NsiI | ATGCAT | 1 cut(s) | 458 |
| NspI | RCATGY | 3 cut(s) | 184, 1400, 1971 |
| NspV | TTCGAA | 1 cut(s) | 538 |
| PaeI | GCATGC | 1 cut(s) | 184 |
| PagI | TCATGA | 2 cut(s) | 208, 1273 |
| PciI | ACATGT | 1 cut(s) | 1967 |
| PciSI | GCTCTTC | 1 cut(s) | 759 |
| PctI | GAATGC | 2 cut(s) | 928, 1078 |
| PfeI | GAWTC | 7 cut(s) | 13, 703, 944, 1216, 1240, 1418, 1709 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1355, 1421 |
| PfoI | TCCNGGA | 1 cut(s) | 1354 |
| PkrI | GCNGC | 6 cut(s) | 1057, 1144, 1345, 1905, 1916, 2008 |
| PscI | ACATGT | 1 cut(s) | 1967 |
| PshBI | ATTAAT | 1 cut(s) | 531 |
| Psp124BI | GAGCTC | 1 cut(s) | 504 |
| Psp6I | CCWGG | 3 cut(s) | 542, 1354, 1579 |
| PspGI | CCWGG | 3 cut(s) | 542, 1354, 1579 |
| PspN4I | GGNNCC | 4 cut(s) | 68, 84, 111, 1805 |
| PspPI | GGNCC | 4 cut(s) | 110, 579, 754, 1120 |
| PstI | CTGCAG | 2 cut(s) | 910, 2008 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1355, 1421, 1510 |
| PsuI | RGATCY | 3 cut(s) | 508, 522, 829 |
| PvuII | CAGCTG | 1 cut(s) | 1343 |
| RsaI | GTAC | 4 cut(s) | 335, 1401, 1753, 1805 |
| RsaNI | GTAC | 4 cut(s) | 334, 1400, 1752, 1804 |
| RseI | CAYNNNNRTG | 4 cut(s) | 432, 489, 684, 1826 |
| SacI | GAGCTC | 1 cut(s) | 504 |
| SapI | GCTCTTC | 1 cut(s) | 759 |
| SaqAI | TTAA | 4 cut(s) | 422, 531, 716, 1281 |
| SatI | GCNGC | 6 cut(s) | 1056, 1143, 1344, 1904, 1915, 2007 |
| Sau3AI | GATC | 9 cut(s) | 283, 508, 517, 522, 729, 829, 954, 1276, 1814 |
| Sau96I | GGNCC | 4 cut(s) | 110, 579, 754, 1120 |
| ScrFI | CCNGG | 5 cut(s) | 87, 544, 1123, 1356, 1581 |
| SduI | GDGCHC | 4 cut(s) | 197, 504, 681, 697 |
| SfaNI | GCATC | 8 cut(s) | 284, 465, 493, 628, 1129, 1308, 1597, 1921 |
| SfcI | CTRYAG | 4 cut(s) | 790, 906, 1851, 2004 |
| SfuI | TTCGAA | 1 cut(s) | 538 |
| SinI | GGWCC | 3 cut(s) | 110, 579, 754 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 432, 489, 684, 1826 |
| SmlI | CTYRAG | 2 cut(s) | 158, 698 |
| SmoI | CTYRAG | 2 cut(s) | 158, 698 |
| SphI | GCATGC | 1 cut(s) | 184 |
| Sse9I | AATT | 9 cut(s) | 96, 345, 423, 647, 777, 1359, 1554, 1559, 2012 |
| SsiI | CCGC | 2 cut(s) | 46, 1988 |
| SspMI | CTAG | 3 cut(s) | 1046, 1146, 1430 |
| SstI | GAGCTC | 1 cut(s) | 504 |
| StyD4I | CCNGG | 5 cut(s) | 85, 542, 1121, 1354, 1579 |
| StyI | CCWWGG | 5 cut(s) | 582, 601, 623, 655, 1807 |
| TaiI | ACGT | 3 cut(s) | 258, 1951, 1977 |
| TaqI | TCGA | 4 cut(s) | 5, 323, 538, 558 |
| TasI | AATT | 9 cut(s) | 96, 345, 423, 647, 777, 1359, 1554, 1559, 2012 |
| TatI | WGTACW | 1 cut(s) | 1399 |
| TfiI | GAWTC | 7 cut(s) | 13, 703, 944, 1216, 1240, 1418, 1709 |
| Tru1I | TTAA | 4 cut(s) | 422, 531, 716, 1281 |
| Tru9I | TTAA | 4 cut(s) | 422, 531, 716, 1281 |
| TseFI | GTSAC | 2 cut(s) | 746, 1191 |
| TseI | GCWGC | 6 cut(s) | 1055, 1142, 1343, 1903, 1914, 2006 |
| Tsp45I | GTSAC | 2 cut(s) | 746, 1191 |
| TspDTI | ATGAA | 8 cut(s) | 63, 197, 702, 777, 983, 1262, 1945, 1953 |
| TspGWI | ACGGA | 2 cut(s) | 107, 937 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1355, 1421 |
| VneI | GTGCAC | 1 cut(s) | 193 |
| VpaK11BI | GGWCC | 3 cut(s) | 110, 579, 754 |
| VspI | ATTAAT | 1 cut(s) | 531 |
| XapI | RAATTY | 3 cut(s) | 647, 1559, 2012 |
| XceI | RCATGY | 3 cut(s) | 184, 1400, 1971 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 413, 662, 1255, 2032 |
| XspI | CTAG | 3 cut(s) | 1046, 1146, 1430 |
| Zsp2I | ATGCAT | 1 cut(s) | 458 |