Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 471 |
| AccII | CGCG | 2 cut(s) | 41, 435 |
| AciI | CCGC | 3 cut(s) | 5, 41, 533 |
| AclWI | GGATC | 2 cut(s) | 222, 716 |
| AcoI | YGGCCR | 1 cut(s) | 743 |
| AcsI | RAATTY | 1 cut(s) | 175 |
| AcyI | GRCGYC | 1 cut(s) | 468 |
| AfaI | GTAC | 2 cut(s) | 432, 651 |
| AfeI | AGCGCT | 1 cut(s) | 356 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 482 |
| AflIII | ACRYGT | 1 cut(s) | 493 |
| AgsI | TTSAA | 4 cut(s) | 224, 491, 554, 698 |
| AjnI | CCWGG | 1 cut(s) | 655 |
| AluBI | AGCT | 1 cut(s) | 677 |
| AluI | AGCT | 1 cut(s) | 677 |
| Alw26I | GTCTC | 2 cut(s) | 249, 661 |
| AlwI | GGATC | 2 cut(s) | 222, 716 |
| Ama87I | CYCGRG | 1 cut(s) | 350 |
| Aor51HI | AGCGCT | 1 cut(s) | 356 |
| AoxI | GGCC | 2 cut(s) | 573, 743 |
| ApeKI | GCWGC | 2 cut(s) | 266, 530 |
| ApoI | RAATTY | 1 cut(s) | 175 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 11, 43 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 179 |
| AspLEI | GCGC | 3 cut(s) | 271, 357, 503 |
| AspS9I | GGNCC | 1 cut(s) | 627 |
| AsuHPI | GGTGA | 2 cut(s) | 54, 112 |
| AsuII | TTCGAA | 1 cut(s) | 127 |
| AvaI | CYCGRG | 1 cut(s) | 350 |
| AvaII | GGWCC | 1 cut(s) | 627 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 391, 423 |
| BbvI | GCAGC | 2 cut(s) | 253, 517 |
| BccI | CCATC | 5 cut(s) | 147, 151, 419, 526, 638 |
| BceAI | ACGGC | 1 cut(s) | 676 |
| BciT130I | CCWGG | 1 cut(s) | 657 |
| BcoDI | GTCTC | 2 cut(s) | 249, 661 |
| BfaI | CTAG | 2 cut(s) | 464, 593 |
| BfoI | RGCGCY | 1 cut(s) | 358 |
| BisI | GCNGC | 3 cut(s) | 42, 267, 531 |
| BlsI | GCNGC | 3 cut(s) | 43, 268, 532 |
| Bme1390I | CCNGG | 1 cut(s) | 657 |
| Bme18I | GGWCC | 1 cut(s) | 627 |
| BmeT110I | CYCGRG | 1 cut(s) | 350 |
| BmgT120I | GGNCC | 1 cut(s) | 627 |
| BmiI | GGNNCC | 3 cut(s) | 141, 628, 690 |
| BmrFI | CCNGG | 1 cut(s) | 657 |
| BmsI | GCATC | 2 cut(s) | 155, 590 |
| BoxI | GACNNNNGTC | 1 cut(s) | 12 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 637, 669 |
| Bpu10I | CCTNAGC | 1 cut(s) | 477 |
| Bpu14I | TTCGAA | 1 cut(s) | 127 |
| BsaHI | GRCGYC | 1 cut(s) | 468 |
| BsaJI | CCNNGG | 2 cut(s) | 249, 656 |
| BsaWI | WCCGGW | 1 cut(s) | 211 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 677, 707 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 482 |
| Bse118I | RCCGGY | 1 cut(s) | 741 |
| Bse1I | ACTGG | 1 cut(s) | 263 |
| Bse3DI | GCAATG | 1 cut(s) | 531 |
| BseBI | CCWGG | 1 cut(s) | 657 |
| BseDI | CCNNGG | 2 cut(s) | 249, 656 |
| BseGI | GGATG | 4 cut(s) | 162, 245, 313, 471 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 482 |
| BseMI | GCAATG | 1 cut(s) | 531 |
| BseMII | CTCAG | 6 cut(s) | 147, 468, 471, 519, 561, 633 |
| BseNI | ACTGG | 1 cut(s) | 263 |
| BseRI | GAGGAG | 4 cut(s) | 119, 122, 625, 701 |
| BseXI | GCAGC | 2 cut(s) | 253, 517 |
| BsgI | GTGCAG | 1 cut(s) | 396 |
| Bsh1236I | CGCG | 2 cut(s) | 41, 435 |
| Bsh1285I | CGRYCG | 2 cut(s) | 187, 439 |
| BshFI | GGCC | 2 cut(s) | 575, 745 |
| BsiEI | CGRYCG | 2 cut(s) | 187, 439 |
| BsiHKCI | CYCGRG | 1 cut(s) | 350 |
| BsiSI | CCGG | 2 cut(s) | 212, 742 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 482 |
| BsmAI | GTCTC | 2 cut(s) | 249, 661 |
| BsnI | GGCC | 2 cut(s) | 575, 745 |
| BsoBI | CYCGRG | 1 cut(s) | 350 |
| Bsp119I | TTCGAA | 1 cut(s) | 127 |
| Bsp143I | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| BspACI | CCGC | 3 cut(s) | 5, 41, 533 |
| BspANI | GGCC | 2 cut(s) | 575, 745 |
| BspCNI | CTCAG | 6 cut(s) | 146, 469, 470, 518, 562, 634 |
| BspFNI | CGCG | 2 cut(s) | 41, 435 |
| BspHI | TCATGA | 1 cut(s) | 82 |
| BspLI | GGNNCC | 3 cut(s) | 141, 628, 690 |
| BspPI | GGATC | 2 cut(s) | 222, 716 |
| BspT104I | TTCGAA | 1 cut(s) | 127 |
| BsrDI | GCAATG | 1 cut(s) | 531 |
| BsrFI | RCCGGY | 1 cut(s) | 741 |
| BsrI | ACTGG | 1 cut(s) | 263 |
| BssAI | RCCGGY | 1 cut(s) | 741 |
| BssECI | CCNNGG | 2 cut(s) | 249, 656 |
| BssMI | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| BssNI | GRCGYC | 1 cut(s) | 468 |
| BssT1I | CCWWGG | 1 cut(s) | 249 |
| Bst2UI | CCWGG | 1 cut(s) | 657 |
| Bst4CI | ACNGT | 1 cut(s) | 550 |
| Bst6I | CTCTTC | 1 cut(s) | 643 |
| BstACI | GRCGYC | 1 cut(s) | 468 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 530 |
| BstBI | TTCGAA | 1 cut(s) | 127 |
| BstC8I | GCNNGC | 1 cut(s) | 321 |
| BstDEI | CTNAG | 7 cut(s) | 133, 230, 457, 477, 505, 570, 642 |
| BstF5I | GGATG | 4 cut(s) | 162, 245, 313, 471 |
| BstFNI | CGCG | 2 cut(s) | 41, 435 |
| BstH2I | RGCGCY | 1 cut(s) | 358 |
| BstHHI | GCGC | 3 cut(s) | 271, 357, 503 |
| BstKTI | GATC | 5 cut(s) | 88, 217, 296, 439, 724 |
| BstMAI | GTCTC | 2 cut(s) | 249, 661 |
| BstMBI | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| BstMCI | CGRYCG | 2 cut(s) | 187, 439 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 38, 530 |
| BstNI | CCWGG | 1 cut(s) | 657 |
| BstNSI | RCATGY | 2 cut(s) | 497, 608 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 12 |
| BstSCI | CCNGG | 1 cut(s) | 655 |
| BstUI | CGCG | 2 cut(s) | 41, 435 |
| BstV1I | GCAGC | 2 cut(s) | 253, 517 |
| BstX2I | RGATCY | 1 cut(s) | 214 |
| BstYI | RGATCY | 1 cut(s) | 214 |
| BsuRI | GGCC | 2 cut(s) | 575, 745 |
| BtsCI | GGATG | 4 cut(s) | 162, 245, 313, 471 |
| Cac8I | GCNNGC | 1 cut(s) | 321 |
| CciI | TCATGA | 1 cut(s) | 82 |
| CfoI | GCGC | 3 cut(s) | 271, 357, 503 |
| Cfr10I | RCCGGY | 1 cut(s) | 741 |
| Cfr13I | GGNCC | 1 cut(s) | 627 |
| CseI | GACGC | 1 cut(s) | 17 |
| Csp6I | GTAC | 2 cut(s) | 431, 650 |
| CviAII | CATG | 3 cut(s) | 83, 494, 605 |
| CviQI | GTAC | 2 cut(s) | 431, 650 |
| DdeI | CTNAG | 7 cut(s) | 133, 230, 457, 477, 505, 570, 642 |
| DpnI | GATC | 5 cut(s) | 87, 216, 295, 438, 723 |
| DpnII | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| EaeI | YGGCCR | 1 cut(s) | 743 |
| Eam1104I | CTCTTC | 1 cut(s) | 643 |
| EarI | CTCTTC | 1 cut(s) | 643 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Eco130I | CCWWGG | 1 cut(s) | 249 |
| Eco147I | AGGCCT | 1 cut(s) | 575 |
| Eco47I | GGWCC | 1 cut(s) | 627 |
| Eco47III | AGCGCT | 1 cut(s) | 356 |
| Eco88I | CYCGRG | 1 cut(s) | 350 |
| EcoRII | CCWGG | 1 cut(s) | 655 |
| EcoT14I | CCWWGG | 1 cut(s) | 249 |
| EcoT22I | ATGCAT | 1 cut(s) | 583 |
| ErhI | CCWWGG | 1 cut(s) | 249 |
| FaeI | CATG | 3 cut(s) | 86, 497, 608 |
| FaiI | YATR | 9 cut(s) | 84, 329, 446, 495, 579, 590, 606, 718, 720 |
| FatI | CATG | 3 cut(s) | 82, 493, 604 |
| FauNDI | CATATG | 1 cut(s) | 718 |
| Fnu4HI | GCNGC | 3 cut(s) | 42, 267, 531 |
| FokI | GGATG | 4 cut(s) | 169, 232, 320, 458 |
| Fsp4HI | GCNGC | 3 cut(s) | 42, 267, 531 |
| FspBI | CTAG | 2 cut(s) | 464, 593 |
| GlaI | GCGC | 3 cut(s) | 270, 356, 502 |
| GluI | GCNGC | 3 cut(s) | 42, 267, 531 |
| HaeII | RGCGCY | 1 cut(s) | 358 |
| HaeIII | GGCC | 2 cut(s) | 575, 745 |
| HapII | CCGG | 2 cut(s) | 212, 742 |
| HgaI | GACGC | 1 cut(s) | 17 |
| HhaI | GCGC | 3 cut(s) | 271, 357, 503 |
| Hin1I | GRCGYC | 1 cut(s) | 468 |
| Hin1II | CATG | 3 cut(s) | 86, 497, 608 |
| Hin6I | GCGC | 3 cut(s) | 269, 355, 501 |
| HinP1I | GCGC | 3 cut(s) | 269, 355, 501 |
| HincII | GTYRAC | 1 cut(s) | 739 |
| HindII | GTYRAC | 1 cut(s) | 739 |
| HinfI | GANTC | 1 cut(s) | 129 |
| HpaII | CCGG | 2 cut(s) | 212, 742 |
| HphI | GGTGA | 2 cut(s) | 54, 112 |
| Hpy166II | GTNNAC | 3 cut(s) | 347, 650, 739 |
| Hpy188I | TCNGA | 8 cut(s) | 55, 136, 206, 441, 460, 508, 643, 666 |
| Hpy188III | TCNNGA | 1 cut(s) | 83 |
| Hpy8I | GTNNAC | 3 cut(s) | 347, 650, 739 |
| HpyAV | CCTTC | 1 cut(s) | 378 |
| HpyCH4III | ACNGT | 1 cut(s) | 550 |
| HpyCH4IV | ACGT | 1 cut(s) | 468 |
| HpyCH4V | TGCA | 5 cut(s) | 168, 413, 451, 545, 581 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 38, 530 |
| HpyF3I | CTNAG | 7 cut(s) | 133, 230, 457, 477, 505, 570, 642 |
| HpySE526I | ACGT | 1 cut(s) | 468 |
| Hsp92I | GRCGYC | 1 cut(s) | 468 |
| Hsp92II | CATG | 3 cut(s) | 86, 497, 608 |
| HspAI | GCGC | 3 cut(s) | 269, 355, 501 |
| Kzo9I | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| LmnI | GCTCC | 3 cut(s) | 418, 688, 707 |
| Lsp1109I | GCAGC | 2 cut(s) | 253, 517 |
| LweI | GCATC | 2 cut(s) | 155, 590 |
| MaeI | CTAG | 2 cut(s) | 464, 593 |
| MaeII | ACGT | 1 cut(s) | 468 |
| MaeIII | GTNAC | 2 cut(s) | 118, 190 |
| MalI | GATC | 5 cut(s) | 87, 216, 295, 438, 723 |
| MboI | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| MboII | GAAGA | 4 cut(s) | 379, 399, 576, 630 |
| MflI | RGATCY | 1 cut(s) | 214 |
| MluCI | AATT | 1 cut(s) | 175 |
| MmeI | TCCRAC | 1 cut(s) | 729 |
| Mph1103I | ATGCAT | 1 cut(s) | 583 |
| MroXI | GAANNNNTTC | 1 cut(s) | 179 |
| MseI | TTAA | 2 cut(s) | 69, 614 |
| MspI | CCGG | 2 cut(s) | 212, 742 |
| MspR9I | CCNGG | 1 cut(s) | 657 |
| MvaI | CCWGG | 1 cut(s) | 657 |
| MvnI | CGCG | 2 cut(s) | 41, 435 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 38, 530 |
| NdeI | CATATG | 1 cut(s) | 718 |
| NdeII | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| NlaIII | CATG | 3 cut(s) | 86, 497, 608 |
| NlaIV | GGNNCC | 3 cut(s) | 141, 628, 690 |
| NmuCI | GTSAC | 1 cut(s) | 118 |
| NsiI | ATGCAT | 1 cut(s) | 583 |
| NspI | RCATGY | 2 cut(s) | 497, 608 |
| NspV | TTCGAA | 1 cut(s) | 127 |
| PaeR7I | CTCGAG | 1 cut(s) | 350 |
| PagI | TCATGA | 1 cut(s) | 82 |
| PceI | AGGCCT | 1 cut(s) | 575 |
| PciI | ACATGT | 1 cut(s) | 493 |
| PdmI | GAANNNNTTC | 1 cut(s) | 179 |
| PfeI | GAWTC | 1 cut(s) | 129 |
| PkrI | GCNGC | 3 cut(s) | 43, 268, 532 |
| Ple19I | CGATCG | 1 cut(s) | 439 |
| PscI | ACATGT | 1 cut(s) | 493 |
| PshAI | GACNNNNGTC | 1 cut(s) | 12 |
| Psp6I | CCWGG | 1 cut(s) | 655 |
| PspGI | CCWGG | 1 cut(s) | 655 |
| PspN4I | GGNNCC | 3 cut(s) | 141, 628, 690 |
| PspPI | GGNCC | 1 cut(s) | 627 |
| PspXI | VCTCGAGB | 1 cut(s) | 350 |
| PsuI | RGATCY | 1 cut(s) | 214 |
| PvuI | CGATCG | 1 cut(s) | 439 |
| RsaI | GTAC | 2 cut(s) | 432, 651 |
| RsaNI | GTAC | 2 cut(s) | 431, 650 |
| SaqAI | TTAA | 2 cut(s) | 69, 614 |
| SatI | GCNGC | 3 cut(s) | 42, 267, 531 |
| Sau3AI | GATC | 5 cut(s) | 85, 214, 293, 436, 721 |
| Sau96I | GGNCC | 1 cut(s) | 627 |
| ScrFI | CCNGG | 1 cut(s) | 657 |
| SetI | ASST | 6 cut(s) | 381, 389, 458, 471, 655, 679 |
| SfaNI | GCATC | 2 cut(s) | 155, 590 |
| Sfr274I | CTCGAG | 1 cut(s) | 350 |
| SfuI | TTCGAA | 1 cut(s) | 127 |
| SinI | GGWCC | 1 cut(s) | 627 |
| SlaI | CTCGAG | 1 cut(s) | 350 |
| SmlI | CTYRAG | 1 cut(s) | 350 |
| SmoI | CTYRAG | 1 cut(s) | 350 |
| Sse9I | AATT | 1 cut(s) | 175 |
| SseBI | AGGCCT | 1 cut(s) | 575 |
| SsiI | CCGC | 3 cut(s) | 5, 41, 533 |
| SspMI | CTAG | 2 cut(s) | 464, 593 |
| StuI | AGGCCT | 1 cut(s) | 575 |
| StyD4I | CCNGG | 1 cut(s) | 655 |
| StyI | CCWWGG | 1 cut(s) | 249 |
| TaaI | ACNGT | 1 cut(s) | 550 |
| TaiI | ACGT | 1 cut(s) | 471 |
| TaqI | TCGA | 4 cut(s) | 127, 151, 351, 365 |
| TaqII | GACCGA | 1 cut(s) | 173 |
| TasI | AATT | 1 cut(s) | 175 |
| TauI | GCSGC | 1 cut(s) | 44 |
| TfiI | GAWTC | 1 cut(s) | 129 |
| Tru1I | TTAA | 2 cut(s) | 69, 614 |
| Tru9I | TTAA | 2 cut(s) | 69, 614 |
| TseFI | GTSAC | 1 cut(s) | 118 |
| TseI | GCWGC | 2 cut(s) | 266, 530 |
| Tsp45I | GTSAC | 1 cut(s) | 118 |
| TspDTI | ATGAA | 1 cut(s) | 71 |
| VpaK11BI | GGWCC | 1 cut(s) | 627 |
| XapI | RAATTY | 1 cut(s) | 175 |
| XceI | RCATGY | 2 cut(s) | 497, 608 |
| XhoI | CTCGAG | 1 cut(s) | 350 |
| XmnI | GAANNNNTTC | 1 cut(s) | 179 |
| XspI | CTAG | 2 cut(s) | 464, 593 |
| ZraI | GACGTC | 1 cut(s) | 469 |
| Zsp2I | ATGCAT | 1 cut(s) | 583 |