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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 100 |
| AccI | GTMKAC | 1 cut(s) | 614 |
| AclWI | GGATC | 2 cut(s) | 222, 287 |
| AcoI | YGGCCR | 1 cut(s) | 247 |
| AcsI | RAATTY | 1 cut(s) | 129 |
| AcyI | GRCGYC | 2 cut(s) | 57, 209 |
| AdeI | CACNNNGTG | 1 cut(s) | 692 |
| AfaI | GTAC | 3 cut(s) | 330, 501, 507 |
| AflII | CTTAAG | 1 cut(s) | 758 |
| AgsI | TTSAA | 1 cut(s) | 778 |
| AhlI | ACTAGT | 1 cut(s) | 502 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 704, 736 |
| AluBI | AGCT | 9 cut(s) | 8, 44, 389, 482, 605, 671, 754, 767, 844 |
| AluI | AGCT | 9 cut(s) | 8, 44, 389, 482, 605, 671, 754, 767, 844 |
| Alw21I | GWGCWC | 2 cut(s) | 522, 820 |
| Alw26I | GTCTC | 2 cut(s) | 382, 739 |
| AlwI | GGATC | 2 cut(s) | 222, 287 |
| AoxI | GGCC | 3 cut(s) | 247, 452, 647 |
| ApeKI | GCWGC | 9 cut(s) | 5, 8, 41, 355, 401, 464, 754, 788, 791 |
| ApoI | RAATTY | 1 cut(s) | 129 |
| AspS9I | GGNCC | 1 cut(s) | 421 |
| AsuHPI | GGTGA | 2 cut(s) | 289, 420 |
| AvaII | GGWCC | 1 cut(s) | 421 |
| AxyI | CCTNAGG | 1 cut(s) | 783 |
| BalI | TGGCCA | 1 cut(s) | 249 |
| BanII | GRGCYC | 1 cut(s) | 159 |
| BbsI | GAAGAC | 1 cut(s) | 376 |
| Bbv12I | GWGCWC | 2 cut(s) | 522, 820 |
| BbvI | GCAGC | 8 cut(s) | 17, 53, 367, 413, 451, 741, 775, 803 |
| BccI | CCATC | 1 cut(s) | 131 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 499, 533 |
| BcoDI | GTCTC | 2 cut(s) | 382, 739 |
| BcuI | ACTAGT | 1 cut(s) | 502 |
| BfaI | CTAG | 2 cut(s) | 503, 584 |
| BfmI | CTRYAG | 3 cut(s) | 399, 615, 789 |
| BfrI | CTTAAG | 1 cut(s) | 758 |
| BfuAI | ACCTGC | 1 cut(s) | 100 |
| BisI | GCNGC | 9 cut(s) | 6, 9, 42, 356, 402, 465, 755, 789, 792 |
| BlsI | GCNGC | 9 cut(s) | 7, 10, 43, 357, 403, 466, 756, 790, 793 |
| BmcAI | AGTACT | 1 cut(s) | 501 |
| Bme18I | GGWCC | 1 cut(s) | 421 |
| BmgT120I | GGNCC | 1 cut(s) | 421 |
| BmiI | GGNNCC | 1 cut(s) | 141 |
| BmrI | ACTGGG | 1 cut(s) | 291 |
| BmsI | GCATC | 1 cut(s) | 451 |
| BmuI | ACTGGG | 1 cut(s) | 291 |
| BoxI | GACNNNNGTC | 1 cut(s) | 564 |
| BpiI | GAAGAC | 1 cut(s) | 376 |
| BpmI | CTGGAG | 2 cut(s) | 684, 711 |
| Bpu10I | CCTNAGC | 1 cut(s) | 672 |
| Bsa29I | ATCGAT | 1 cut(s) | 525 |
| BsaAI | YACGTR | 1 cut(s) | 797 |
| BsaBI | GATNNNNATC | 1 cut(s) | 122 |
| BsaHI | GRCGYC | 2 cut(s) | 57, 209 |
| BsaI | GGTCTC | 1 cut(s) | 382 |
| BsaJI | CCNNGG | 1 cut(s) | 151 |
| Bse118I | RCCGGY | 2 cut(s) | 222, 490 |
| Bse1I | ACTGG | 6 cut(s) | 297, 424, 431, 515, 603, 728 |
| Bse21I | CCTNAGG | 1 cut(s) | 783 |
| Bse3DI | GCAATG | 1 cut(s) | 419 |
| Bse8I | GATNNNNATC | 1 cut(s) | 122 |
| BseCI | ATCGAT | 1 cut(s) | 525 |
| BseDI | CCNNGG | 1 cut(s) | 151 |
| BseJI | GATNNNNATC | 1 cut(s) | 122 |
| BseMI | GCAATG | 1 cut(s) | 419 |
| BseMII | CTCAG | 2 cut(s) | 393, 686 |
| BseNI | ACTGG | 6 cut(s) | 297, 424, 431, 515, 603, 728 |
| BseRI | GAGGAG | 3 cut(s) | 122, 152, 673 |
| BseXI | GCAGC | 8 cut(s) | 17, 53, 367, 413, 451, 741, 775, 803 |
| BsgI | GTGCAG | 1 cut(s) | 247 |
| BshFI | GGCC | 3 cut(s) | 249, 454, 649 |
| BshVI | ATCGAT | 1 cut(s) | 525 |
| BsiHKAI | GWGCWC | 2 cut(s) | 522, 820 |
| BsiSI | CCGG | 3 cut(s) | 212, 223, 491 |
| BslFI | GGGAC | 3 cut(s) | 181, 287, 552 |
| BsmAI | GTCTC | 2 cut(s) | 382, 739 |
| BsmBI | CGTCTC | 1 cut(s) | 739 |
| BsmFI | GGGAC | 3 cut(s) | 181, 287, 552 |
| BsnI | GGCC | 3 cut(s) | 249, 454, 649 |
| Bso31I | GGTCTC | 1 cut(s) | 382 |
| Bsp1286I | GDGCHC | 3 cut(s) | 159, 522, 820 |
| Bsp143I | GATC | 2 cut(s) | 214, 292 |
| BspANI | GGCC | 3 cut(s) | 249, 454, 649 |
| BspCNI | CTCAG | 2 cut(s) | 392, 685 |
| BspDI | ATCGAT | 1 cut(s) | 525 |
| BspLI | GGNNCC | 1 cut(s) | 141 |
| BspMAI | CTGCAG | 2 cut(s) | 403, 793 |
| BspMI | ACCTGC | 1 cut(s) | 100 |
| BspPI | GGATC | 2 cut(s) | 222, 287 |
| BspTI | CTTAAG | 1 cut(s) | 758 |
| BspTNI | GGTCTC | 1 cut(s) | 382 |
| BsrDI | GCAATG | 1 cut(s) | 419 |
| BsrFI | RCCGGY | 2 cut(s) | 222, 490 |
| BsrI | ACTGG | 6 cut(s) | 297, 424, 431, 515, 603, 728 |
| BssAI | RCCGGY | 2 cut(s) | 222, 490 |
| BssECI | CCNNGG | 1 cut(s) | 151 |
| BssMI | GATC | 2 cut(s) | 214, 292 |
| BssNI | GRCGYC | 2 cut(s) | 57, 209 |
| Bst4CI | ACNGT | 4 cut(s) | 350, 394, 541, 643 |
| BstACI | GRCGYC | 2 cut(s) | 57, 209 |
| BstAFI | CTTAAG | 1 cut(s) | 758 |
| BstBAI | YACGTR | 1 cut(s) | 797 |
| BstC8I | GCNNGC | 3 cut(s) | 13, 399, 765 |
| BstDEI | CTNAG | 4 cut(s) | 379, 672, 689, 783 |
| BstKTI | GATC | 2 cut(s) | 217, 295 |
| BstMAI | GTCTC | 2 cut(s) | 382, 739 |
| BstMBI | GATC | 2 cut(s) | 214, 292 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 352, 760 |
| BstNSI | RCATGY | 1 cut(s) | 440 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 564 |
| BstSFI | CTRYAG | 3 cut(s) | 399, 615, 789 |
| BstV1I | GCAGC | 8 cut(s) | 17, 53, 367, 413, 451, 741, 775, 803 |
| BstV2I | GAAGAC | 1 cut(s) | 376 |
| BstX2I | RGATCY | 1 cut(s) | 214 |
| BstYI | RGATCY | 1 cut(s) | 214 |
| Bsu15I | ATCGAT | 1 cut(s) | 525 |
| Bsu36I | CCTNAGG | 1 cut(s) | 783 |
| BsuRI | GGCC | 3 cut(s) | 249, 454, 649 |
| BsuTUI | ATCGAT | 1 cut(s) | 525 |
| BtsIMutI | CAGTG | 4 cut(s) | 304, 438, 522, 816 |
| BveI | ACCTGC | 1 cut(s) | 100 |
| Cac8I | GCNNGC | 3 cut(s) | 13, 399, 765 |
| Cfr10I | RCCGGY | 2 cut(s) | 222, 490 |
| Cfr13I | GGNCC | 1 cut(s) | 421 |
| ClaI | ATCGAT | 1 cut(s) | 525 |
| CseI | GACGC | 3 cut(s) | 65, 217, 327 |
| Csp6I | GTAC | 3 cut(s) | 329, 500, 506 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 474, 509 |
| CviAII | CATG | 4 cut(s) | 251, 437, 535, 625 |
| CviQI | GTAC | 3 cut(s) | 329, 500, 506 |
| DdeI | CTNAG | 4 cut(s) | 379, 672, 689, 783 |
| DpnI | GATC | 2 cut(s) | 216, 294 |
| DpnII | GATC | 2 cut(s) | 214, 292 |
| DraIII | CACNNNGTG | 1 cut(s) | 692 |
| EaeI | YGGCCR | 1 cut(s) | 247 |
| Eco147I | AGGCCT | 1 cut(s) | 454 |
| Eco24I | GRGCYC | 1 cut(s) | 159 |
| Eco31I | GGTCTC | 1 cut(s) | 382 |
| Eco32I | GATATC | 1 cut(s) | 120 |
| Eco47I | GGWCC | 1 cut(s) | 421 |
| Eco81I | CCTNAGG | 1 cut(s) | 783 |
| EcoRV | GATATC | 1 cut(s) | 120 |
| EcoT38I | GRGCYC | 1 cut(s) | 159 |
| Esp3I | CGTCTC | 1 cut(s) | 739 |
| FaeI | CATG | 4 cut(s) | 254, 440, 538, 628 |
| FaiI | YATR | 9 cut(s) | 39, 102, 252, 342, 438, 536, 579, 608, 626 |
| FaqI | GGGAC | 3 cut(s) | 181, 287, 552 |
| FatI | CATG | 4 cut(s) | 250, 436, 534, 624 |
| FblI | GTMKAC | 1 cut(s) | 614 |
| Fnu4HI | GCNGC | 9 cut(s) | 6, 9, 42, 356, 402, 465, 755, 789, 792 |
| FriOI | GRGCYC | 1 cut(s) | 159 |
| Fsp4HI | GCNGC | 9 cut(s) | 6, 9, 42, 356, 402, 465, 755, 789, 792 |
| FspBI | CTAG | 2 cut(s) | 503, 584 |
| GluI | GCNGC | 9 cut(s) | 6, 9, 42, 356, 402, 465, 755, 789, 792 |
| GsuI | CTGGAG | 2 cut(s) | 684, 711 |
| HaeIII | GGCC | 3 cut(s) | 249, 454, 649 |
| HapII | CCGG | 3 cut(s) | 212, 223, 491 |
| HgaI | GACGC | 3 cut(s) | 65, 217, 327 |
| Hin1I | GRCGYC | 2 cut(s) | 57, 209 |
| Hin1II | CATG | 4 cut(s) | 254, 440, 538, 628 |
| HindIII | AAGCTT | 1 cut(s) | 842 |
| HinfI | GANTC | 2 cut(s) | 30, 677 |
| HpaII | CCGG | 3 cut(s) | 212, 223, 491 |
| HphI | GGTGA | 2 cut(s) | 289, 420 |
| Hpy166II | GTNNAC | 3 cut(s) | 615, 664, 723 |
| Hpy188I | TCNGA | 3 cut(s) | 55, 152, 421 |
| Hpy188III | TCNNGA | 4 cut(s) | 205, 584, 674, 701 |
| Hpy8I | GTNNAC | 3 cut(s) | 615, 664, 723 |
| HpyAV | CCTTC | 2 cut(s) | 77, 707 |
| HpyCH4III | ACNGT | 4 cut(s) | 350, 394, 541, 643 |
| HpyCH4IV | ACGT | 3 cut(s) | 194, 331, 796 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 352, 760 |
| HpyF3I | CTNAG | 4 cut(s) | 379, 672, 689, 783 |
| HpySE526I | ACGT | 3 cut(s) | 194, 331, 796 |
| Hsp92I | GRCGYC | 2 cut(s) | 57, 209 |
| Hsp92II | CATG | 4 cut(s) | 254, 440, 538, 628 |
| Kzo9I | GATC | 2 cut(s) | 214, 292 |
| LmnI | GCTCC | 3 cut(s) | 100, 139, 487 |
| Lsp1109I | GCAGC | 8 cut(s) | 17, 53, 367, 413, 451, 741, 775, 803 |
| LweI | GCATC | 1 cut(s) | 451 |
| MaeI | CTAG | 2 cut(s) | 503, 584 |
| MaeII | ACGT | 3 cut(s) | 194, 331, 796 |
| MaeIII | GTNAC | 3 cut(s) | 190, 426, 769 |
| MalI | GATC | 2 cut(s) | 216, 294 |
| MboI | GATC | 2 cut(s) | 214, 292 |
| MboII | GAAGA | 1 cut(s) | 381 |
| MflI | RGATCY | 1 cut(s) | 214 |
| MhlI | GDGCHC | 3 cut(s) | 159, 522, 820 |
| MlsI | TGGCCA | 1 cut(s) | 249 |
| MluCI | AATT | 1 cut(s) | 129 |
| MluNI | TGGCCA | 1 cut(s) | 249 |
| MmeI | TCCRAC | 1 cut(s) | 574 |
| Mox20I | TGGCCA | 1 cut(s) | 249 |
| MscI | TGGCCA | 1 cut(s) | 249 |
| MseI | TTAA | 1 cut(s) | 759 |
| Msp20I | TGGCCA | 1 cut(s) | 249 |
| MspA1I | CMGCKG | 1 cut(s) | 8 |
| MspCI | CTTAAG | 1 cut(s) | 758 |
| MspI | CCGG | 3 cut(s) | 212, 223, 491 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 352, 760 |
| NdeII | GATC | 2 cut(s) | 214, 292 |
| NlaIII | CATG | 4 cut(s) | 254, 440, 538, 628 |
| NlaIV | GGNNCC | 1 cut(s) | 141 |
| NmuCI | GTSAC | 1 cut(s) | 426 |
| NspI | RCATGY | 1 cut(s) | 440 |
| PceI | AGGCCT | 1 cut(s) | 454 |
| PfeI | GAWTC | 2 cut(s) | 30, 677 |
| PkrI | GCNGC | 9 cut(s) | 7, 10, 43, 357, 403, 466, 756, 790, 793 |
| Ppu21I | YACGTR | 1 cut(s) | 797 |
| PshAI | GACNNNNGTC | 1 cut(s) | 564 |
| PspN4I | GGNNCC | 1 cut(s) | 141 |
| PspPI | GGNCC | 1 cut(s) | 421 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 343, 375 |
| PstI | CTGCAG | 2 cut(s) | 403, 793 |
| PsuI | RGATCY | 1 cut(s) | 214 |
| PvuII | CAGCTG | 1 cut(s) | 8 |
| RsaI | GTAC | 3 cut(s) | 330, 501, 507 |
| RsaNI | GTAC | 3 cut(s) | 329, 500, 506 |
| SaqAI | TTAA | 1 cut(s) | 759 |
| SatI | GCNGC | 9 cut(s) | 6, 9, 42, 356, 402, 465, 755, 789, 792 |
| Sau3AI | GATC | 2 cut(s) | 214, 292 |
| Sau96I | GGNCC | 1 cut(s) | 421 |
| ScaI | AGTACT | 1 cut(s) | 501 |
| SduI | GDGCHC | 3 cut(s) | 159, 522, 820 |
| SfaNI | GCATC | 1 cut(s) | 451 |
| SfcI | CTRYAG | 3 cut(s) | 399, 615, 789 |
| SinI | GGWCC | 1 cut(s) | 421 |
| SmlI | CTYRAG | 1 cut(s) | 758 |
| SmoI | CTYRAG | 1 cut(s) | 758 |
| SpeI | ACTAGT | 1 cut(s) | 502 |
| Sse9I | AATT | 1 cut(s) | 129 |
| SseBI | AGGCCT | 1 cut(s) | 454 |
| SspMI | CTAG | 2 cut(s) | 503, 584 |
| StuI | AGGCCT | 1 cut(s) | 454 |
| TaaI | ACNGT | 4 cut(s) | 350, 394, 541, 643 |
| TaiI | ACGT | 3 cut(s) | 197, 334, 799 |
| TaqI | TCGA | 2 cut(s) | 159, 525 |
| TasI | AATT | 1 cut(s) | 129 |
| TatI | WGTACW | 1 cut(s) | 499 |
| TfiI | GAWTC | 2 cut(s) | 30, 677 |
| Tru1I | TTAA | 1 cut(s) | 759 |
| Tru9I | TTAA | 1 cut(s) | 759 |
| TscAI | CASTG | 4 cut(s) | 304, 438, 522, 816 |
| TseFI | GTSAC | 1 cut(s) | 426 |
| TseI | GCWGC | 9 cut(s) | 5, 8, 41, 355, 401, 464, 754, 788, 791 |
| Tsp45I | GTSAC | 1 cut(s) | 426 |
| TspRI | CASTG | 4 cut(s) | 304, 438, 522, 816 |
| Vha464I | CTTAAG | 1 cut(s) | 758 |
| VpaK11BI | GGWCC | 1 cut(s) | 421 |
| XapI | RAATTY | 1 cut(s) | 129 |
| XbaI | TCTAGA | 1 cut(s) | 583 |
| XceI | RCATGY | 1 cut(s) | 440 |
| XmiI | GTMKAC | 1 cut(s) | 614 |
| XspI | CTAG | 2 cut(s) | 503, 584 |
| ZrmI | AGTACT | 1 cut(s) | 501 |