No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 477 |
| AciI | CCGC | 3 cut(s) | 629, 676, 682 |
| AclWI | GGATC | 3 cut(s) | 249, 420, 433 |
| AcsI | RAATTY | 2 cut(s) | 208, 339 |
| AfaI | GTAC | 2 cut(s) | 119, 552 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 232 |
| AgsI | TTSAA | 1 cut(s) | 335 |
| AjnI | CCWGG | 1 cut(s) | 473 |
| AluBI | AGCT | 5 cut(s) | 136, 143, 197, 250, 596 |
| AluI | AGCT | 5 cut(s) | 136, 143, 197, 250, 596 |
| Alw21I | GWGCWC | 1 cut(s) | 252 |
| AlwI | GGATC | 3 cut(s) | 249, 420, 433 |
| ApoI | RAATTY | 2 cut(s) | 208, 339 |
| AseI | ATTAAT | 3 cut(s) | 276, 447, 570 |
| AspLEI | GCGC | 1 cut(s) | 33 |
| AspS9I | GGNCC | 2 cut(s) | 223, 508 |
| AsuHPI | GGTGA | 2 cut(s) | 283, 549 |
| AvaII | GGWCC | 2 cut(s) | 223, 508 |
| BamHI | GGATCC | 1 cut(s) | 425 |
| BanI | GGYRCC | 1 cut(s) | 477 |
| BanII | GRGCYC | 1 cut(s) | 252 |
| Bbv12I | GWGCWC | 1 cut(s) | 252 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 242, 276 |
| BciT130I | CCWGG | 1 cut(s) | 475 |
| BfaI | CTAG | 3 cut(s) | 186, 348, 597 |
| BfmI | CTRYAG | 1 cut(s) | 636 |
| Bme1390I | CCNGG | 1 cut(s) | 475 |
| Bme18I | GGWCC | 2 cut(s) | 223, 508 |
| BmgT120I | GGNCC | 2 cut(s) | 223, 508 |
| BmiI | GGNNCC | 2 cut(s) | 427, 479 |
| BmrFI | CCNGG | 1 cut(s) | 475 |
| BmsI | GCATC | 2 cut(s) | 42, 406 |
| BpuEI | CTTGAG | 1 cut(s) | 215 |
| BsaJI | CCNNGG | 1 cut(s) | 390 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 120, 150 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 232 |
| Bse1I | ACTGG | 1 cut(s) | 284 |
| BseBI | CCWGG | 1 cut(s) | 475 |
| BseDI | CCNNGG | 1 cut(s) | 390 |
| BseGI | GGATG | 3 cut(s) | 148, 400, 421 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 232 |
| BseMII | CTCAG | 3 cut(s) | 189, 308, 545 |
| BseNI | ACTGG | 1 cut(s) | 284 |
| Bsh1285I | CGRYCG | 1 cut(s) | 256 |
| BshNI | GGYRCC | 1 cut(s) | 477 |
| BsiEI | CGRYCG | 1 cut(s) | 256 |
| BsiHKAI | GWGCWC | 1 cut(s) | 252 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 232 |
| Bsp1286I | GDGCHC | 1 cut(s) | 252 |
| Bsp1407I | TGTACA | 1 cut(s) | 550 |
| Bsp143I | GATC | 4 cut(s) | 19, 241, 253, 425 |
| BspACI | CCGC | 3 cut(s) | 629, 676, 682 |
| BspCNI | CTCAG | 3 cut(s) | 190, 307, 544 |
| BspLI | GGNNCC | 2 cut(s) | 427, 479 |
| BspMAI | CTGCAG | 1 cut(s) | 640 |
| BspPI | GGATC | 3 cut(s) | 249, 420, 433 |
| BspT107I | GGYRCC | 1 cut(s) | 477 |
| BsrGI | TGTACA | 1 cut(s) | 550 |
| BsrI | ACTGG | 1 cut(s) | 284 |
| BssECI | CCNNGG | 1 cut(s) | 390 |
| BssMI | GATC | 4 cut(s) | 19, 241, 253, 425 |
| Bst2UI | CCWGG | 1 cut(s) | 475 |
| Bst4CI | ACNGT | 2 cut(s) | 71, 122 |
| BstAUI | TGTACA | 1 cut(s) | 550 |
| BstC8I | GCNNGC | 1 cut(s) | 29 |
| BstDEI | CTNAG | 4 cut(s) | 96, 198, 294, 531 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 230 |
| BstF5I | GGATG | 3 cut(s) | 148, 400, 421 |
| BstHHI | GCGC | 1 cut(s) | 33 |
| BstKTI | GATC | 4 cut(s) | 22, 244, 256, 428 |
| BstMBI | GATC | 4 cut(s) | 19, 241, 253, 425 |
| BstMCI | CGRYCG | 1 cut(s) | 256 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 602, 635 |
| BstNI | CCWGG | 1 cut(s) | 475 |
| BstSCI | CCNGG | 1 cut(s) | 473 |
| BstSFI | CTRYAG | 1 cut(s) | 636 |
| BstX2I | RGATCY | 1 cut(s) | 425 |
| BstYI | RGATCY | 1 cut(s) | 425 |
| BtsCI | GGATG | 3 cut(s) | 148, 400, 421 |
| BtsIMutI | CAGTG | 1 cut(s) | 291 |
| Cac8I | GCNNGC | 1 cut(s) | 29 |
| CfoI | GCGC | 1 cut(s) | 33 |
| Cfr13I | GGNCC | 2 cut(s) | 223, 508 |
| CseI | GACGC | 1 cut(s) | 533 |
| Csp6I | GTAC | 2 cut(s) | 118, 551 |
| CviAII | CATG | 1 cut(s) | 542 |
| CviQI | GTAC | 2 cut(s) | 118, 551 |
| DdeI | CTNAG | 4 cut(s) | 96, 198, 294, 531 |
| DpnI | GATC | 4 cut(s) | 21, 243, 255, 427 |
| DpnII | GATC | 4 cut(s) | 19, 241, 253, 425 |
| Ecl136II | GAGCTC | 1 cut(s) | 250 |
| Eco24I | GRGCYC | 1 cut(s) | 252 |
| Eco47I | GGWCC | 2 cut(s) | 223, 508 |
| Eco53kI | GAGCTC | 1 cut(s) | 250 |
| EcoICRI | GAGCTC | 1 cut(s) | 250 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 230 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 1 cut(s) | 473 |
| EcoT38I | GRGCYC | 1 cut(s) | 252 |
| FaeI | CATG | 1 cut(s) | 545 |
| FaiI | YATR | 5 cut(s) | 269, 320, 432, 543, 612 |
| FatI | CATG | 1 cut(s) | 541 |
| FauI | CCCGC | 1 cut(s) | 622 |
| FokI | GGATG | 3 cut(s) | 135, 407, 428 |
| FriOI | GRGCYC | 1 cut(s) | 252 |
| FspBI | CTAG | 3 cut(s) | 186, 348, 597 |
| GlaI | GCGC | 1 cut(s) | 32 |
| HgaI | GACGC | 1 cut(s) | 533 |
| HhaI | GCGC | 1 cut(s) | 33 |
| Hin1II | CATG | 1 cut(s) | 545 |
| Hin6I | GCGC | 1 cut(s) | 31 |
| HinP1I | GCGC | 1 cut(s) | 31 |
| HincII | GTYRAC | 1 cut(s) | 49 |
| HindII | GTYRAC | 1 cut(s) | 49 |
| HinfI | GANTC | 2 cut(s) | 202, 518 |
| HpaI | GTTAAC | 1 cut(s) | 49 |
| HphI | GGTGA | 2 cut(s) | 283, 549 |
| Hpy166II | GTNNAC | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 5 cut(s) | 165, 207, 394, 415, 523 |
| Hpy188III | TCNNGA | 1 cut(s) | 245 |
| Hpy8I | GTNNAC | 1 cut(s) | 49 |
| Hpy99I | CGWCG | 1 cut(s) | 527 |
| HpyCH4III | ACNGT | 2 cut(s) | 71, 122 |
| HpyCH4V | TGCA | 3 cut(s) | 27, 215, 638 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 602, 635 |
| HpyF3I | CTNAG | 4 cut(s) | 96, 198, 294, 531 |
| Hsp92II | CATG | 1 cut(s) | 545 |
| HspAI | GCGC | 1 cut(s) | 31 |
| KspAI | GTTAAC | 1 cut(s) | 49 |
| Kzo9I | GATC | 4 cut(s) | 19, 241, 253, 425 |
| LmnI | GCTCC | 2 cut(s) | 141, 247 |
| LpnPI | CCDG | 5 cut(s) | 114, 230, 297, 460, 487 |
| LweI | GCATC | 2 cut(s) | 42, 406 |
| MaeI | CTAG | 3 cut(s) | 186, 348, 597 |
| MaeIII | GTNAC | 2 cut(s) | 286, 534 |
| MalI | GATC | 4 cut(s) | 21, 243, 255, 427 |
| MboI | GATC | 4 cut(s) | 19, 241, 253, 425 |
| MboII | GAAGA | 3 cut(s) | 94, 336, 506 |
| MfeI | CAATTG | 1 cut(s) | 500 |
| MflI | RGATCY | 1 cut(s) | 425 |
| MhlI | GDGCHC | 1 cut(s) | 252 |
| MluCI | AATT | 8 cut(s) | 52, 208, 258, 339, 439, 500, 571, 613 |
| MlyI | GAGTC | 1 cut(s) | 527 |
| MmeI | TCCRAC | 1 cut(s) | 546 |
| MnlI | CCTC | 6 cut(s) | 162, 189, 400, 484, 499, 595 |
| MseI | TTAA | 6 cut(s) | 48, 66, 276, 447, 570, 642 |
| MspR9I | CCNGG | 1 cut(s) | 475 |
| MunI | CAATTG | 1 cut(s) | 500 |
| MvaI | CCWGG | 1 cut(s) | 475 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 602, 635 |
| NdeII | GATC | 4 cut(s) | 19, 241, 253, 425 |
| NlaIII | CATG | 1 cut(s) | 545 |
| NlaIV | GGNNCC | 2 cut(s) | 427, 479 |
| NmuCI | GTSAC | 2 cut(s) | 286, 534 |
| PfeI | GAWTC | 1 cut(s) | 202 |
| PflFI | GACNNNGTC | 1 cut(s) | 221 |
| Ple19I | CGATCG | 1 cut(s) | 256 |
| PleI | GAGTC | 1 cut(s) | 526 |
| PpsI | GAGTC | 1 cut(s) | 526 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| PshBI | ATTAAT | 3 cut(s) | 276, 447, 570 |
| Psp124BI | GAGCTC | 1 cut(s) | 252 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 1 cut(s) | 473 |
| PspGI | CCWGG | 1 cut(s) | 473 |
| PspN4I | GGNNCC | 2 cut(s) | 427, 479 |
| PspPI | GGNCC | 2 cut(s) | 223, 508 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PstI | CTGCAG | 1 cut(s) | 640 |
| PsuI | RGATCY | 1 cut(s) | 425 |
| PsyI | GACNNNGTC | 1 cut(s) | 221 |
| PvuI | CGATCG | 1 cut(s) | 256 |
| RsaI | GTAC | 2 cut(s) | 119, 552 |
| RsaNI | GTAC | 2 cut(s) | 118, 551 |
| SacI | GAGCTC | 1 cut(s) | 252 |
| SaqAI | TTAA | 6 cut(s) | 48, 66, 276, 447, 570, 642 |
| Sau3AI | GATC | 4 cut(s) | 19, 241, 253, 425 |
| Sau96I | GGNCC | 2 cut(s) | 223, 508 |
| SchI | GAGTC | 1 cut(s) | 527 |
| ScrFI | CCNGG | 1 cut(s) | 475 |
| SduI | GDGCHC | 1 cut(s) | 252 |
| SfaNI | GCATC | 2 cut(s) | 42, 406 |
| SfcI | CTRYAG | 1 cut(s) | 636 |
| SinI | GGWCC | 2 cut(s) | 223, 508 |
| SmlI | CTYRAG | 1 cut(s) | 230 |
| SmoI | CTYRAG | 1 cut(s) | 230 |
| Sse9I | AATT | 8 cut(s) | 52, 208, 258, 339, 439, 500, 571, 613 |
| SsiI | CCGC | 3 cut(s) | 629, 676, 682 |
| SspI | AATATT | 1 cut(s) | 568 |
| SspMI | CTAG | 3 cut(s) | 186, 348, 597 |
| SstI | GAGCTC | 1 cut(s) | 252 |
| StyD4I | CCNGG | 1 cut(s) | 473 |
| TaaI | ACNGT | 2 cut(s) | 71, 122 |
| TaqI | TCGA | 2 cut(s) | 252, 256 |
| TasI | AATT | 8 cut(s) | 52, 208, 258, 339, 439, 500, 571, 613 |
| TatI | WGTACW | 1 cut(s) | 550 |
| TfiI | GAWTC | 1 cut(s) | 202 |
| Tru1I | TTAA | 6 cut(s) | 48, 66, 276, 447, 570, 642 |
| Tru9I | TTAA | 6 cut(s) | 48, 66, 276, 447, 570, 642 |
| TscAI | CASTG | 1 cut(s) | 291 |
| TseFI | GTSAC | 2 cut(s) | 286, 534 |
| Tsp45I | GTSAC | 2 cut(s) | 286, 534 |
| TspDTI | ATGAA | 2 cut(s) | 414, 558 |
| TspRI | CASTG | 1 cut(s) | 291 |
| Tth111I | GACNNNGTC | 1 cut(s) | 221 |
| VpaK11BI | GGWCC | 2 cut(s) | 223, 508 |
| VspI | ATTAAT | 3 cut(s) | 276, 447, 570 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 230 |
| XapI | RAATTY | 2 cut(s) | 208, 339 |
| XspI | CTAG | 3 cut(s) | 186, 348, 597 |