Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 222 |
| Acc65I | GGTACC | 1 cut(s) | 79 |
| AccB1I | GGYRCC | 2 cut(s) | 10, 79 |
| AciI | CCGC | 3 cut(s) | 175, 439, 970 |
| AclWI | GGATC | 4 cut(s) | 65, 117, 1019, 1032 |
| AcsI | RAATTY | 1 cut(s) | 335 |
| AcuI | CTGAAG | 3 cut(s) | 147, 665, 792 |
| AfaI | GTAC | 5 cut(s) | 81, 405, 502, 718, 817 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 181, 673 |
| AgsI | TTSAA | 9 cut(s) | 75, 271, 341, 422, 791, 851, 987, 1128, 1164 |
| AjnI | CCWGG | 1 cut(s) | 83 |
| AluBI | AGCT | 5 cut(s) | 188, 344, 710, 785, 886 |
| AluI | AGCT | 5 cut(s) | 188, 344, 710, 785, 886 |
| Alw21I | GWGCWC | 1 cut(s) | 23 |
| Alw26I | GTCTC | 2 cut(s) | 124, 581 |
| Alw44I | GTGCAC | 1 cut(s) | 19 |
| AlwI | GGATC | 4 cut(s) | 65, 117, 1019, 1032 |
| AoxI | GGCC | 1 cut(s) | 555 |
| ApaLI | GTGCAC | 1 cut(s) | 19 |
| ApeKI | GCWGC | 2 cut(s) | 185, 436 |
| ApoI | RAATTY | 1 cut(s) | 335 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 366, 786 |
| Asp718I | GGTACC | 1 cut(s) | 79 |
| AspLEI | GCGC | 1 cut(s) | 310 |
| AspS9I | GGNCC | 3 cut(s) | 245, 676, 1066 |
| AsuHPI | GGTGA | 2 cut(s) | 275, 752 |
| AvaII | GGWCC | 3 cut(s) | 245, 676, 1066 |
| BaeGI | GKGCMC | 2 cut(s) | 23, 970 |
| BamHI | GGATCC | 1 cut(s) | 1024 |
| BanI | GGYRCC | 2 cut(s) | 10, 79 |
| BanII | GRGCYC | 2 cut(s) | 289, 568 |
| BbsI | GAAGAC | 2 cut(s) | 636, 975 |
| Bbv12I | GWGCWC | 1 cut(s) | 23 |
| BbvI | GCAGC | 2 cut(s) | 197, 448 |
| BccI | CCATC | 1 cut(s) | 909 |
| BceAI | ACGGC | 1 cut(s) | 198 |
| BciT130I | CCWGG | 1 cut(s) | 85 |
| BcoDI | GTCTC | 2 cut(s) | 124, 581 |
| BfaI | CTAG | 4 cut(s) | 102, 455, 906, 1077 |
| BfoI | RGCGCY | 1 cut(s) | 311 |
| BfuAI | ACCTGC | 1 cut(s) | 222 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 563 |
| BisI | GCNGC | 2 cut(s) | 186, 437 |
| BlsI | GCNGC | 2 cut(s) | 187, 438 |
| Bme1390I | CCNGG | 1 cut(s) | 85 |
| Bme18I | GGWCC | 3 cut(s) | 245, 676, 1066 |
| BmgT120I | GGNCC | 3 cut(s) | 245, 676, 1066 |
| BmiI | GGNNCC | 3 cut(s) | 12, 81, 1026 |
| BmrFI | CCNGG | 1 cut(s) | 85 |
| BpiI | GAAGAC | 2 cut(s) | 636, 975 |
| BsaBI | GATNNNNATC | 4 cut(s) | 528, 747, 919, 1008 |
| BsaJI | CCNNGG | 6 cut(s) | 83, 84, 180, 248, 509, 658 |
| BsaWI | WCCGGW | 2 cut(s) | 582, 1168 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 297, 327 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 181, 673 |
| Bse1I | ACTGG | 2 cut(s) | 247, 1099 |
| Bse3DI | GCAATG | 1 cut(s) | 514 |
| Bse8I | GATNNNNATC | 4 cut(s) | 528, 747, 919, 1008 |
| BseBI | CCWGG | 1 cut(s) | 85 |
| BseDI | CCNNGG | 6 cut(s) | 83, 84, 180, 248, 509, 658 |
| BseGI | GGATG | 1 cut(s) | 981 |
| BseJI | GATNNNNATC | 4 cut(s) | 528, 747, 919, 1008 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 181, 673 |
| BseMI | GCAATG | 1 cut(s) | 514 |
| BseMII | CTCAG | 3 cut(s) | 381, 534, 971 |
| BseNI | ACTGG | 2 cut(s) | 247, 1099 |
| BseSI | GKGCMC | 2 cut(s) | 23, 970 |
| BseXI | GCAGC | 2 cut(s) | 197, 448 |
| BsgI | GTGCAG | 1 cut(s) | 351 |
| BshFI | GGCC | 1 cut(s) | 557 |
| BshNI | GGYRCC | 2 cut(s) | 10, 79 |
| BsiHKAI | GWGCWC | 1 cut(s) | 23 |
| BsiSI | CCGG | 2 cut(s) | 583, 1169 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 181, 673 |
| BsmAI | GTCTC | 2 cut(s) | 124, 581 |
| BsnI | GGCC | 1 cut(s) | 557 |
| Bsp1286I | GDGCHC | 4 cut(s) | 23, 289, 568, 970 |
| Bsp143I | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| Bsp19I | CCATGG | 2 cut(s) | 248, 658 |
| BspACI | CCGC | 3 cut(s) | 175, 439, 970 |
| BspANI | GGCC | 1 cut(s) | 557 |
| BspCNI | CTCAG | 3 cut(s) | 382, 533, 970 |
| BspHI | TCATGA | 2 cut(s) | 289, 630 |
| BspLI | GGNNCC | 3 cut(s) | 12, 81, 1026 |
| BspMI | ACCTGC | 1 cut(s) | 222 |
| BspPI | GGATC | 4 cut(s) | 65, 117, 1019, 1032 |
| BspQI | GCTCTTC | 1 cut(s) | 792 |
| BspT107I | GGYRCC | 2 cut(s) | 10, 79 |
| BsrDI | GCAATG | 1 cut(s) | 514 |
| BsrI | ACTGG | 2 cut(s) | 247, 1099 |
| BssECI | CCNNGG | 6 cut(s) | 83, 84, 180, 248, 509, 658 |
| BssMI | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| BssT1I | CCWWGG | 2 cut(s) | 248, 658 |
| Bst2UI | CCWGG | 1 cut(s) | 85 |
| Bst4CI | ACNGT | 1 cut(s) | 145 |
| Bst6I | CTCTTC | 3 cut(s) | 406, 584, 792 |
| BstC8I | GCNNGC | 3 cut(s) | 175, 568, 970 |
| BstDEI | CTNAG | 6 cut(s) | 390, 463, 520, 552, 778, 957 |
| BstDSI | CCRYGG | 3 cut(s) | 180, 248, 658 |
| BstF5I | GGATG | 1 cut(s) | 981 |
| BstH2I | RGCGCY | 1 cut(s) | 311 |
| BstHHI | GCGC | 1 cut(s) | 310 |
| BstKTI | GATC | 8 cut(s) | 31, 60, 112, 526, 769, 934, 1027, 1187 |
| BstMAI | GTCTC | 2 cut(s) | 124, 581 |
| BstMBI | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 464, 563, 782, 892, 1082 |
| BstNI | CCWGG | 1 cut(s) | 85 |
| BstNSI | RCATGY | 1 cut(s) | 797 |
| BstSCI | CCNGG | 1 cut(s) | 83 |
| BstSLI | GKGCMC | 2 cut(s) | 23, 970 |
| BstV1I | GCAGC | 2 cut(s) | 197, 448 |
| BstV2I | GAAGAC | 2 cut(s) | 636, 975 |
| BstX2I | RGATCY | 2 cut(s) | 57, 1024 |
| BstYI | RGATCY | 2 cut(s) | 57, 1024 |
| BsuRI | GGCC | 1 cut(s) | 557 |
| BtgI | CCRYGG | 3 cut(s) | 180, 248, 658 |
| BtgZI | GCGATG | 1 cut(s) | 928 |
| BtsCI | GGATG | 1 cut(s) | 981 |
| BtsI | GCAGTG | 1 cut(s) | 282 |
| BtsIMutI | CAGTG | 2 cut(s) | 282, 965 |
| BveI | ACCTGC | 1 cut(s) | 222 |
| Cac8I | GCNNGC | 3 cut(s) | 175, 568, 970 |
| CciI | TCATGA | 2 cut(s) | 289, 630 |
| CfoI | GCGC | 1 cut(s) | 310 |
| Cfr13I | GGNCC | 3 cut(s) | 245, 676, 1066 |
| Csp6I | GTAC | 5 cut(s) | 80, 404, 501, 717, 816 |
| CviQI | GTAC | 5 cut(s) | 80, 404, 501, 717, 816 |
| DdeI | CTNAG | 6 cut(s) | 390, 463, 520, 552, 778, 957 |
| DpnI | GATC | 8 cut(s) | 30, 59, 111, 525, 768, 933, 1026, 1186 |
| DpnII | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| DraI | TTTAAA | 2 cut(s) | 619, 861 |
| Eam1104I | CTCTTC | 3 cut(s) | 406, 584, 792 |
| EarI | CTCTTC | 3 cut(s) | 406, 584, 792 |
| Eco130I | CCWWGG | 2 cut(s) | 248, 658 |
| Eco24I | GRGCYC | 2 cut(s) | 289, 568 |
| Eco47I | GGWCC | 3 cut(s) | 245, 676, 1066 |
| Eco57I | CTGAAG | 3 cut(s) | 147, 665, 792 |
| EcoRII | CCWGG | 1 cut(s) | 83 |
| EcoT14I | CCWWGG | 2 cut(s) | 248, 658 |
| EcoT38I | GRGCYC | 2 cut(s) | 289, 568 |
| ErhI | CCWWGG | 2 cut(s) | 248, 658 |
| FauI | CCCGC | 2 cut(s) | 182, 977 |
| Fnu4HI | GCNGC | 2 cut(s) | 186, 437 |
| FokI | GGATG | 1 cut(s) | 988 |
| FriOI | GRGCYC | 2 cut(s) | 289, 568 |
| Fsp4HI | GCNGC | 2 cut(s) | 186, 437 |
| FspBI | CTAG | 4 cut(s) | 102, 455, 906, 1077 |
| GlaI | GCGC | 1 cut(s) | 309 |
| GluI | GCNGC | 2 cut(s) | 186, 437 |
| HaeII | RGCGCY | 1 cut(s) | 311 |
| HaeIII | GGCC | 1 cut(s) | 557 |
| HapII | CCGG | 2 cut(s) | 583, 1169 |
| HhaI | GCGC | 1 cut(s) | 310 |
| Hin6I | GCGC | 1 cut(s) | 308 |
| HinP1I | GCGC | 1 cut(s) | 308 |
| HincII | GTYRAC | 1 cut(s) | 320 |
| HindII | GTYRAC | 1 cut(s) | 320 |
| HinfI | GANTC | 3 cut(s) | 866, 1042, 1120 |
| HpaII | CCGG | 2 cut(s) | 583, 1169 |
| HphI | GGTGA | 2 cut(s) | 275, 752 |
| Hpy166II | GTNNAC | 5 cut(s) | 21, 207, 320, 376, 396 |
| Hpy188I | TCNGA | 4 cut(s) | 352, 523, 925, 1178 |
| Hpy188III | TCNNGA | 4 cut(s) | 290, 631, 1001, 1039 |
| Hpy8I | GTNNAC | 5 cut(s) | 21, 207, 320, 376, 396 |
| HpyAV | CCTTC | 6 cut(s) | 421, 556, 689, 920, 1134, 1173 |
| HpyCH4III | ACNGT | 1 cut(s) | 145 |
| HpyCH4IV | ACGT | 1 cut(s) | 503 |
| HpyCH4V | TGCA | 6 cut(s) | 21, 275, 332, 797, 833, 1085 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 464, 563, 782, 892, 1082 |
| HpyF3I | CTNAG | 6 cut(s) | 390, 463, 520, 552, 778, 957 |
| HpySE526I | ACGT | 1 cut(s) | 503 |
| HspAI | GCGC | 1 cut(s) | 308 |
| KpnI | GGTACC | 1 cut(s) | 83 |
| Kzo9I | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| LguI | GCTCTTC | 1 cut(s) | 792 |
| LmnI | GCTCC | 2 cut(s) | 305, 604 |
| Lsp1109I | GCAGC | 2 cut(s) | 197, 448 |
| MaeI | CTAG | 4 cut(s) | 102, 455, 906, 1077 |
| MaeII | ACGT | 1 cut(s) | 503 |
| MaeIII | GTNAC | 2 cut(s) | 238, 1132 |
| MalI | GATC | 8 cut(s) | 30, 59, 111, 525, 768, 933, 1026, 1186 |
| MboI | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| MflI | RGATCY | 2 cut(s) | 57, 1024 |
| MhlI | GDGCHC | 4 cut(s) | 23, 289, 568, 970 |
| MluCI | AATT | 9 cut(s) | 148, 335, 362, 634, 724, 760, 798, 856, 1164 |
| MlyI | GAGTC | 1 cut(s) | 875 |
| MmeI | TCCRAC | 3 cut(s) | 375, 423, 582 |
| MnlI | CCTC | 7 cut(s) | 407, 519, 543, 678, 724, 972, 1015 |
| MroXI | GAANNNNTTC | 2 cut(s) | 366, 786 |
| MseI | TTAA | 6 cut(s) | 225, 539, 618, 692, 860, 872 |
| MslI | CAYNNNNRTG | 1 cut(s) | 753 |
| MspA1I | CMGCKG | 2 cut(s) | 188, 439 |
| MspI | CCGG | 2 cut(s) | 583, 1169 |
| MspR9I | CCNGG | 1 cut(s) | 85 |
| MvaI | CCWGG | 1 cut(s) | 85 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 464, 563, 782, 892, 1082 |
| NcoI | CCATGG | 2 cut(s) | 248, 658 |
| NdeII | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| NlaIV | GGNNCC | 3 cut(s) | 12, 81, 1026 |
| NmeAIII | GCCGAG | 1 cut(s) | 490 |
| NspI | RCATGY | 1 cut(s) | 797 |
| PagI | TCATGA | 2 cut(s) | 289, 630 |
| PasI | CCCWGGG | 1 cut(s) | 84 |
| PciSI | GCTCTTC | 1 cut(s) | 792 |
| PdmI | GAANNNNTTC | 2 cut(s) | 366, 786 |
| PfeI | GAWTC | 2 cut(s) | 1042, 1120 |
| PkrI | GCNGC | 2 cut(s) | 187, 438 |
| PleI | GAGTC | 1 cut(s) | 874 |
| PpsI | GAGTC | 1 cut(s) | 874 |
| Psp6I | CCWGG | 1 cut(s) | 83 |
| PspGI | CCWGG | 1 cut(s) | 83 |
| PspN4I | GGNNCC | 3 cut(s) | 12, 81, 1026 |
| PspPI | GGNCC | 3 cut(s) | 245, 676, 1066 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 72, 104 |
| PsuI | RGATCY | 2 cut(s) | 57, 1024 |
| PvuII | CAGCTG | 1 cut(s) | 188 |
| RsaI | GTAC | 5 cut(s) | 81, 405, 502, 718, 817 |
| RsaNI | GTAC | 5 cut(s) | 80, 404, 501, 717, 816 |
| RseI | CAYNNNNRTG | 1 cut(s) | 753 |
| SapI | GCTCTTC | 1 cut(s) | 792 |
| SaqAI | TTAA | 6 cut(s) | 225, 539, 618, 692, 860, 872 |
| SatI | GCNGC | 2 cut(s) | 186, 437 |
| Sau3AI | GATC | 8 cut(s) | 28, 57, 109, 523, 766, 931, 1024, 1184 |
| Sau96I | GGNCC | 3 cut(s) | 245, 676, 1066 |
| SchI | GAGTC | 1 cut(s) | 875 |
| ScrFI | CCNGG | 1 cut(s) | 85 |
| SduI | GDGCHC | 4 cut(s) | 23, 289, 568, 970 |
| SinI | GGWCC | 3 cut(s) | 245, 676, 1066 |
| SmiI | ATTTAAAT | 1 cut(s) | 861 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 753 |
| Sse9I | AATT | 9 cut(s) | 148, 335, 362, 634, 724, 760, 798, 856, 1164 |
| SsiI | CCGC | 3 cut(s) | 175, 439, 970 |
| SspMI | CTAG | 4 cut(s) | 102, 455, 906, 1077 |
| StyD4I | CCNGG | 1 cut(s) | 83 |
| StyI | CCWWGG | 2 cut(s) | 248, 658 |
| SwaI | ATTTAAAT | 1 cut(s) | 861 |
| TaaI | ACNGT | 1 cut(s) | 145 |
| TaiI | ACGT | 1 cut(s) | 506 |
| TaqI | TCGA | 1 cut(s) | 1000 |
| TasI | AATT | 9 cut(s) | 148, 335, 362, 634, 724, 760, 798, 856, 1164 |
| TfiI | GAWTC | 2 cut(s) | 1042, 1120 |
| Tru1I | TTAA | 6 cut(s) | 225, 539, 618, 692, 860, 872 |
| Tru9I | TTAA | 6 cut(s) | 225, 539, 618, 692, 860, 872 |
| TscAI | CASTG | 2 cut(s) | 282, 965 |
| TseI | GCWGC | 2 cut(s) | 185, 436 |
| TspDTI | ATGAA | 3 cut(s) | 306, 359, 647 |
| TspGWI | ACGGA | 2 cut(s) | 122, 704 |
| TspRI | CASTG | 2 cut(s) | 282, 965 |
| VneI | GTGCAC | 1 cut(s) | 19 |
| VpaK11BI | GGWCC | 3 cut(s) | 245, 676, 1066 |
| XapI | RAATTY | 1 cut(s) | 335 |
| XceI | RCATGY | 1 cut(s) | 797 |
| XmnI | GAANNNNTTC | 2 cut(s) | 366, 786 |
| XspI | CTAG | 4 cut(s) | 102, 455, 906, 1077 |