Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 500 |
| Acc36I | ACCTGC | 1 cut(s) | 500 |
| AccB1I | GGYRCC | 4 cut(s) | 4, 240, 278, 607 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 803, 815 |
| AccII | CGCG | 1 cut(s) | 58 |
| AciI | CCGC | 4 cut(s) | 299, 812, 854, 947 |
| AcsI | RAATTY | 1 cut(s) | 89 |
| AcuI | CTGAAG | 2 cut(s) | 25, 825 |
| AcyI | GRCGYC | 3 cut(s) | 5, 181, 199 |
| AfaI | GTAC | 1 cut(s) | 727 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 509, 729, 730, 803, 815, 987 |
| AgsI | TTSAA | 3 cut(s) | 142, 389, 425 |
| AjnI | CCWGG | 1 cut(s) | 814 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 237, 269 |
| AluBI | AGCT | 4 cut(s) | 161, 435, 614, 1022 |
| AluI | AGCT | 4 cut(s) | 161, 435, 614, 1022 |
| Alw21I | GWGCWC | 1 cut(s) | 793 |
| Alw26I | GTCTC | 1 cut(s) | 533 |
| Alw44I | GTGCAC | 1 cut(s) | 789 |
| AoxI | GGCC | 1 cut(s) | 220 |
| ApaLI | GTGCAC | 1 cut(s) | 789 |
| ApeKI | GCWGC | 1 cut(s) | 554 |
| ApoI | RAATTY | 1 cut(s) | 89 |
| AspLEI | GCGC | 1 cut(s) | 7 |
| AspS9I | GGNCC | 4 cut(s) | 191, 205, 220, 806 |
| AsuHPI | GGTGA | 3 cut(s) | 524, 708, 887 |
| AsuII | TTCGAA | 2 cut(s) | 695, 993 |
| AsuNHI | GCTAGC | 1 cut(s) | 431 |
| AvaII | GGWCC | 3 cut(s) | 191, 205, 806 |
| BaeGI | GKGCMC | 3 cut(s) | 131, 281, 793 |
| BanI | GGYRCC | 4 cut(s) | 4, 240, 278, 607 |
| Bbv12I | GWGCWC | 1 cut(s) | 793 |
| BbvCI | CCTCAGC | 1 cut(s) | 615 |
| BbvI | GCAGC | 1 cut(s) | 541 |
| BccI | CCATC | 4 cut(s) | 566, 790, 881, 990 |
| BciT130I | CCWGG | 1 cut(s) | 816 |
| BclI | TGATCA | 1 cut(s) | 517 |
| BcoDI | GTCTC | 1 cut(s) | 533 |
| BfaI | CTAG | 5 cut(s) | 257, 333, 432, 668, 786 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BfuAI | ACCTGC | 1 cut(s) | 500 |
| BglII | AGATCT | 1 cut(s) | 61 |
| BisI | GCNGC | 2 cut(s) | 300, 555 |
| BlsI | GCNGC | 2 cut(s) | 301, 556 |
| Bme1390I | CCNGG | 1 cut(s) | 816 |
| Bme18I | GGWCC | 3 cut(s) | 191, 205, 806 |
| BmgT120I | GGNCC | 4 cut(s) | 191, 205, 220, 806 |
| BmiI | GGNNCC | 7 cut(s) | 6, 192, 207, 242, 280, 609, 780 |
| BmrFI | CCNGG | 1 cut(s) | 816 |
| BmsI | GCATC | 8 cut(s) | 121, 286, 433, 478, 670, 679, 692, 841 |
| BmtI | GCTAGC | 1 cut(s) | 435 |
| BpmI | CTGGAG | 1 cut(s) | 944 |
| Bpu10I | CCTNAGC | 1 cut(s) | 615 |
| Bpu14I | TTCGAA | 2 cut(s) | 695, 993 |
| BsaHI | GRCGYC | 3 cut(s) | 5, 181, 199 |
| BsaJI | CCNNGG | 4 cut(s) | 28, 194, 216, 802 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 509, 729, 730, 803, 815, 987 |
| Bse1I | ACTGG | 1 cut(s) | 899 |
| Bse3DI | GCAATG | 1 cut(s) | 210 |
| BseBI | CCWGG | 1 cut(s) | 816 |
| BseDI | CCNNGG | 4 cut(s) | 28, 194, 216, 802 |
| BseGI | GGATG | 5 cut(s) | 112, 301, 352, 493, 558 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 509, 729, 730, 803, 815, 987 |
| BseMI | GCAATG | 1 cut(s) | 210 |
| BseMII | CTCAG | 1 cut(s) | 606 |
| BseNI | ACTGG | 1 cut(s) | 899 |
| BseRI | GAGGAG | 4 cut(s) | 617, 665, 761, 764 |
| BseSI | GKGCMC | 3 cut(s) | 131, 281, 793 |
| BseXI | GCAGC | 1 cut(s) | 541 |
| Bsh1236I | CGCG | 1 cut(s) | 58 |
| BshFI | GGCC | 1 cut(s) | 222 |
| BshNI | GGYRCC | 4 cut(s) | 4, 240, 278, 607 |
| BsiHKAI | GWGCWC | 1 cut(s) | 793 |
| BslFI | GGGAC | 1 cut(s) | 191 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 509, 729, 730, 803, 815, 987 |
| BsmAI | GTCTC | 1 cut(s) | 533 |
| BsmFI | GGGAC | 1 cut(s) | 191 |
| BsnI | GGCC | 1 cut(s) | 222 |
| Bsp119I | TTCGAA | 2 cut(s) | 695, 993 |
| Bsp1286I | GDGCHC | 3 cut(s) | 131, 281, 793 |
| Bsp143I | GATC | 3 cut(s) | 61, 517, 963 |
| BspACI | CCGC | 4 cut(s) | 299, 812, 854, 947 |
| BspANI | GGCC | 1 cut(s) | 222 |
| BspCNI | CTCAG | 1 cut(s) | 607 |
| BspFNI | CGCG | 1 cut(s) | 58 |
| BspLI | GGNNCC | 7 cut(s) | 6, 192, 207, 242, 280, 609, 780 |
| BspMI | ACCTGC | 1 cut(s) | 500 |
| BspOI | GCTAGC | 1 cut(s) | 435 |
| BspT104I | TTCGAA | 2 cut(s) | 695, 993 |
| BspT107I | GGYRCC | 4 cut(s) | 4, 240, 278, 607 |
| BsrDI | GCAATG | 1 cut(s) | 210 |
| BsrI | ACTGG | 1 cut(s) | 899 |
| BssECI | CCNNGG | 4 cut(s) | 28, 194, 216, 802 |
| BssMI | GATC | 3 cut(s) | 61, 517, 963 |
| BssNI | GRCGYC | 3 cut(s) | 5, 181, 199 |
| BssT1I | CCWWGG | 2 cut(s) | 216, 802 |
| Bst2UI | CCWGG | 1 cut(s) | 816 |
| Bst4CI | ACNGT | 1 cut(s) | 595 |
| BstACI | GRCGYC | 3 cut(s) | 5, 181, 199 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 689 |
| BstBI | TTCGAA | 2 cut(s) | 695, 993 |
| BstC8I | GCNNGC | 2 cut(s) | 433, 1000 |
| BstDEI | CTNAG | 3 cut(s) | 224, 615, 1012 |
| BstDSI | CCRYGG | 2 cut(s) | 28, 194 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 507 |
| BstF5I | GGATG | 5 cut(s) | 112, 301, 352, 493, 558 |
| BstFNI | CGCG | 1 cut(s) | 58 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 1 cut(s) | 7 |
| BstKTI | GATC | 3 cut(s) | 64, 520, 966 |
| BstMAI | GTCTC | 1 cut(s) | 533 |
| BstMBI | GATC | 3 cut(s) | 61, 517, 963 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 689 |
| BstNI | CCWGG | 1 cut(s) | 816 |
| BstNSI | RCATGY | 1 cut(s) | 452 |
| BstSCI | CCNGG | 1 cut(s) | 814 |
| BstSLI | GKGCMC | 3 cut(s) | 131, 281, 793 |
| BstUI | CGCG | 1 cut(s) | 58 |
| BstV1I | GCAGC | 1 cut(s) | 541 |
| BstX2I | RGATCY | 1 cut(s) | 61 |
| BstYI | RGATCY | 1 cut(s) | 61 |
| BsuRI | GGCC | 1 cut(s) | 222 |
| BtgI | CCRYGG | 2 cut(s) | 28, 194 |
| BtsCI | GGATG | 5 cut(s) | 112, 301, 352, 493, 558 |
| BveI | ACCTGC | 1 cut(s) | 500 |
| Cac8I | GCNNGC | 2 cut(s) | 433, 1000 |
| CfoI | GCGC | 1 cut(s) | 7 |
| Cfr13I | GGNCC | 4 cut(s) | 191, 205, 220, 806 |
| CseI | GACGC | 2 cut(s) | 170, 188 |
| Csp6I | GTAC | 1 cut(s) | 726 |
| CviAII | CATG | 2 cut(s) | 419, 449 |
| CviQI | GTAC | 1 cut(s) | 726 |
| DdeI | CTNAG | 3 cut(s) | 224, 615, 1012 |
| DinI | GGCGCC | 1 cut(s) | 6 |
| DpnI | GATC | 3 cut(s) | 63, 519, 965 |
| DpnII | GATC | 3 cut(s) | 61, 517, 963 |
| Eco130I | CCWWGG | 2 cut(s) | 216, 802 |
| Eco47I | GGWCC | 3 cut(s) | 191, 205, 806 |
| Eco57I | CTGAAG | 2 cut(s) | 25, 825 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 507 |
| EcoO109I | RGGNCCY | 1 cut(s) | 220 |
| EcoRII | CCWGG | 1 cut(s) | 814 |
| EcoT14I | CCWWGG | 2 cut(s) | 216, 802 |
| EcoT22I | ATGCAT | 1 cut(s) | 685 |
| EgeI | GGCGCC | 1 cut(s) | 6 |
| EheI | GGCGCC | 1 cut(s) | 6 |
| ErhI | CCWWGG | 2 cut(s) | 216, 802 |
| FaeI | CATG | 2 cut(s) | 422, 452 |
| FaqI | GGGAC | 1 cut(s) | 191 |
| FatI | CATG | 2 cut(s) | 418, 448 |
| FbaI | TGATCA | 1 cut(s) | 517 |
| Fnu4HI | GCNGC | 2 cut(s) | 300, 555 |
| FokI | GGATG | 5 cut(s) | 99, 308, 359, 500, 545 |
| Fsp4HI | GCNGC | 2 cut(s) | 300, 555 |
| FspBI | CTAG | 5 cut(s) | 257, 333, 432, 668, 786 |
| GlaI | GCGC | 1 cut(s) | 6 |
| GluI | GCNGC | 2 cut(s) | 300, 555 |
| GsuI | CTGGAG | 1 cut(s) | 944 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HaeIII | GGCC | 1 cut(s) | 222 |
| HgaI | GACGC | 2 cut(s) | 170, 188 |
| HhaI | GCGC | 1 cut(s) | 7 |
| Hin1I | GRCGYC | 3 cut(s) | 5, 181, 199 |
| Hin1II | CATG | 2 cut(s) | 422, 452 |
| Hin6I | GCGC | 1 cut(s) | 5 |
| HinP1I | GCGC | 1 cut(s) | 5 |
| HincII | GTYRAC | 1 cut(s) | 760 |
| HindII | GTYRAC | 1 cut(s) | 760 |
| HinfI | GANTC | 2 cut(s) | 82, 713 |
| HphI | GGTGA | 3 cut(s) | 524, 708, 887 |
| Hpy166II | GTNNAC | 4 cut(s) | 21, 760, 791, 809 |
| Hpy188I | TCNGA | 6 cut(s) | 81, 231, 270, 442, 688, 844 |
| Hpy188III | TCNNGA | 4 cut(s) | 257, 410, 425, 961 |
| Hpy8I | GTNNAC | 4 cut(s) | 21, 760, 791, 809 |
| HpyAV | CCTTC | 2 cut(s) | 77, 195 |
| HpyCH4III | ACNGT | 1 cut(s) | 595 |
| HpyCH4IV | ACGT | 2 cut(s) | 374, 762 |
| HpyCH4V | TGCA | 8 cut(s) | 13, 112, 124, 289, 446, 683, 791, 860 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 689 |
| HpyF3I | CTNAG | 3 cut(s) | 224, 615, 1012 |
| HpySE526I | ACGT | 2 cut(s) | 374, 762 |
| Hsp92I | GRCGYC | 3 cut(s) | 5, 181, 199 |
| Hsp92II | CATG | 2 cut(s) | 422, 452 |
| HspAI | GCGC | 1 cut(s) | 5 |
| KasI | GGCGCC | 1 cut(s) | 4 |
| Ksp22I | TGATCA | 1 cut(s) | 517 |
| Kzo9I | GATC | 3 cut(s) | 61, 517, 963 |
| LmnI | GCTCC | 2 cut(s) | 784, 1027 |
| Lsp1109I | GCAGC | 1 cut(s) | 541 |
| LweI | GCATC | 8 cut(s) | 121, 286, 433, 478, 670, 679, 692, 841 |
| MaeI | CTAG | 5 cut(s) | 257, 333, 432, 668, 786 |
| MaeII | ACGT | 2 cut(s) | 374, 762 |
| MaeIII | GTNAC | 1 cut(s) | 478 |
| MalI | GATC | 3 cut(s) | 63, 519, 965 |
| MboI | GATC | 3 cut(s) | 61, 517, 963 |
| MboII | GAAGA | 4 cut(s) | 31, 62, 65, 638 |
| MfeI | CAATTG | 1 cut(s) | 290 |
| MflI | RGATCY | 1 cut(s) | 61 |
| MhlI | GDGCHC | 3 cut(s) | 131, 281, 793 |
| MluCI | AATT | 5 cut(s) | 89, 261, 290, 658, 846 |
| Mly113I | GGCGCC | 1 cut(s) | 5 |
| MmeI | TCCRAC | 1 cut(s) | 906 |
| Mph1103I | ATGCAT | 1 cut(s) | 685 |
| MseI | TTAA | 2 cut(s) | 456, 870 |
| MspA1I | CMGCKG | 1 cut(s) | 614 |
| MspR9I | CCNGG | 1 cut(s) | 816 |
| MunI | CAATTG | 1 cut(s) | 290 |
| MvaI | CCWGG | 1 cut(s) | 816 |
| MvnI | CGCG | 1 cut(s) | 58 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 689 |
| NarI | GGCGCC | 1 cut(s) | 5 |
| NdeII | GATC | 3 cut(s) | 61, 517, 963 |
| NheI | GCTAGC | 1 cut(s) | 431 |
| NlaIII | CATG | 2 cut(s) | 422, 452 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 192, 207, 242, 280, 609, 780 |
| NsiI | ATGCAT | 1 cut(s) | 685 |
| NspI | RCATGY | 1 cut(s) | 452 |
| NspV | TTCGAA | 2 cut(s) | 695, 993 |
| PaqCI | CACCTGC | 1 cut(s) | 500 |
| PfeI | GAWTC | 2 cut(s) | 82, 713 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 803, 815 |
| PkrI | GCNGC | 2 cut(s) | 301, 556 |
| PluTI | GGCGCC | 1 cut(s) | 8 |
| Psp6I | CCWGG | 1 cut(s) | 814 |
| PspGI | CCWGG | 1 cut(s) | 814 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 192, 207, 242, 280, 609, 780 |
| PspPI | GGNCC | 4 cut(s) | 191, 205, 220, 806 |
| PsuI | RGATCY | 1 cut(s) | 61 |
| PvuII | CAGCTG | 1 cut(s) | 614 |
| RsaI | GTAC | 1 cut(s) | 727 |
| RsaNI | GTAC | 1 cut(s) | 726 |
| SaqAI | TTAA | 2 cut(s) | 456, 870 |
| SatI | GCNGC | 2 cut(s) | 300, 555 |
| Sau3AI | GATC | 3 cut(s) | 61, 517, 963 |
| Sau96I | GGNCC | 4 cut(s) | 191, 205, 220, 806 |
| ScrFI | CCNGG | 1 cut(s) | 816 |
| SduI | GDGCHC | 3 cut(s) | 131, 281, 793 |
| SfaNI | GCATC | 8 cut(s) | 121, 286, 433, 478, 670, 679, 692, 841 |
| SfoI | GGCGCC | 1 cut(s) | 6 |
| SfuI | TTCGAA | 2 cut(s) | 695, 993 |
| SinI | GGWCC | 3 cut(s) | 191, 205, 806 |
| Sse9I | AATT | 5 cut(s) | 89, 261, 290, 658, 846 |
| SsiI | CCGC | 4 cut(s) | 299, 812, 854, 947 |
| SspDI | GGCGCC | 1 cut(s) | 4 |
| SspI | AATATT | 1 cut(s) | 1037 |
| SspMI | CTAG | 5 cut(s) | 257, 333, 432, 668, 786 |
| StyD4I | CCNGG | 1 cut(s) | 814 |
| StyI | CCWWGG | 2 cut(s) | 216, 802 |
| TaaI | ACNGT | 1 cut(s) | 595 |
| TaiI | ACGT | 2 cut(s) | 377, 765 |
| TaqI | TCGA | 3 cut(s) | 85, 695, 993 |
| TasI | AATT | 5 cut(s) | 89, 261, 290, 658, 846 |
| TauI | GCSGC | 1 cut(s) | 302 |
| TfiI | GAWTC | 2 cut(s) | 82, 713 |
| Tru1I | TTAA | 2 cut(s) | 456, 870 |
| Tru9I | TTAA | 2 cut(s) | 456, 870 |
| TseI | GCWGC | 1 cut(s) | 554 |
| TspDTI | ATGAA | 2 cut(s) | 65, 1032 |
| TspGWI | ACGGA | 4 cut(s) | 183, 407, 483, 997 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 803, 815 |
| VneI | GTGCAC | 1 cut(s) | 789 |
| VpaK11BI | GGWCC | 3 cut(s) | 191, 205, 806 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 507 |
| XapI | RAATTY | 1 cut(s) | 89 |
| XbaI | TCTAGA | 1 cut(s) | 256 |
| XceI | RCATGY | 1 cut(s) | 452 |
| XspI | CTAG | 5 cut(s) | 257, 333, 432, 668, 786 |
| Zsp2I | ATGCAT | 1 cut(s) | 685 |