Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1066 |
| Acc36I | ACCTGC | 3 cut(s) | 777, 1042, 1066 |
| Acc65I | GGTACC | 1 cut(s) | 745 |
| AccB1I | GGYRCC | 1 cut(s) | 745 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 331 |
| AccI | GTMKAC | 2 cut(s) | 600, 803 |
| AccII | CGCG | 2 cut(s) | 159, 900 |
| AccIII | TCCGGA | 1 cut(s) | 955 |
| AciI | CCGC | 3 cut(s) | 159, 253, 370 |
| AclWI | GGATC | 2 cut(s) | 477, 539 |
| AcoI | YGGCCR | 1 cut(s) | 1077 |
| AcsI | RAATTY | 3 cut(s) | 226, 436, 1259 |
| AfaI | GTAC | 2 cut(s) | 747, 967 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 331, 453, 454, 466, 764 |
| AgsI | TTSAA | 6 cut(s) | 34, 134, 226, 538, 674, 1193 |
| AjnI | CCWGG | 3 cut(s) | 409, 447, 1159 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 14, 46 |
| AleI | CACNNNNGTG | 2 cut(s) | 1085, 1240 |
| AluBI | AGCT | 3 cut(s) | 250, 911, 1075 |
| AluI | AGCT | 3 cut(s) | 250, 911, 1075 |
| Alw21I | GWGCWC | 3 cut(s) | 1057, 1092, 1127 |
| Alw26I | GTCTC | 4 cut(s) | 130, 292, 360, 872 |
| Alw44I | GTGCAC | 2 cut(s) | 1053, 1088 |
| AlwI | GGATC | 2 cut(s) | 477, 539 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 881 |
| Ama87I | CYCGRG | 1 cut(s) | 639 |
| Aor13HI | TCCGGA | 1 cut(s) | 955 |
| AoxI | GGCC | 2 cut(s) | 263, 1077 |
| ApaLI | GTGCAC | 2 cut(s) | 1053, 1088 |
| ApoI | RAATTY | 3 cut(s) | 226, 436, 1259 |
| AseI | ATTAAT | 1 cut(s) | 395 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 684 |
| Asp718I | GGTACC | 1 cut(s) | 745 |
| AspLEI | GCGC | 3 cut(s) | 952, 1006, 1039 |
| AspS9I | GGNCC | 1 cut(s) | 457 |
| AsuC2I | CCSGG | 1 cut(s) | 1268 |
| AsuHPI | GGTGA | 2 cut(s) | 1176, 1255 |
| AvaI | CYCGRG | 1 cut(s) | 639 |
| AvaII | GGWCC | 1 cut(s) | 457 |
| BaeGI | GKGCMC | 2 cut(s) | 1057, 1092 |
| BalI | TGGCCA | 1 cut(s) | 1079 |
| BanI | GGYRCC | 1 cut(s) | 745 |
| BauI | CACGAG | 1 cut(s) | 1091 |
| BbsI | GAAGAC | 1 cut(s) | 829 |
| Bbv12I | GWGCWC | 3 cut(s) | 1057, 1092, 1127 |
| BccI | CCATC | 4 cut(s) | 92, 106, 641, 1106 |
| BceAI | ACGGC | 1 cut(s) | 848 |
| BciT130I | CCWGG | 3 cut(s) | 411, 449, 1161 |
| BcnI | CCSGG | 1 cut(s) | 1268 |
| BcoDI | GTCTC | 4 cut(s) | 130, 292, 360, 872 |
| BfaI | CTAG | 2 cut(s) | 110, 582 |
| BfmI | CTRYAG | 1 cut(s) | 48 |
| BfuAI | ACCTGC | 3 cut(s) | 777, 1042, 1066 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1361 |
| BlpI | GCTNAGC | 1 cut(s) | 1062 |
| Bme1390I | CCNGG | 4 cut(s) | 411, 449, 1161, 1268 |
| Bme18I | GGWCC | 1 cut(s) | 457 |
| BmeT110I | CYCGRG | 1 cut(s) | 639 |
| BmgT120I | GGNCC | 1 cut(s) | 457 |
| BmiI | GGNNCC | 3 cut(s) | 147, 458, 747 |
| BmrFI | CCNGG | 4 cut(s) | 411, 449, 1161, 1268 |
| BmsI | GCATC | 2 cut(s) | 519, 961 |
| BpiI | GAAGAC | 1 cut(s) | 829 |
| BpmI | CTGGAG | 1 cut(s) | 87 |
| Bpu10I | CCTNAGC | 1 cut(s) | 912 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1062 |
| BpuEI | CTTGAG | 2 cut(s) | 1220, 1337 |
| BpuMI | CCSGG | 1 cut(s) | 1268 |
| Bsa29I | ATCGAT | 1 cut(s) | 87 |
| BsaBI | GATNNNNATC | 1 cut(s) | 134 |
| BsaI | GGTCTC | 1 cut(s) | 360 |
| BsaJI | CCNNGG | 3 cut(s) | 447, 1100, 1266 |
| BsaWI | WCCGGW | 1 cut(s) | 955 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 331, 453, 454, 466, 764 |
| Bse1I | ACTGG | 4 cut(s) | 104, 284, 325, 951 |
| Bse3DI | GCAATG | 1 cut(s) | 775 |
| Bse8I | GATNNNNATC | 1 cut(s) | 134 |
| BseAI | TCCGGA | 1 cut(s) | 955 |
| BseBI | CCWGG | 3 cut(s) | 411, 449, 1161 |
| BseCI | ATCGAT | 1 cut(s) | 87 |
| BseDI | CCNNGG | 3 cut(s) | 447, 1100, 1266 |
| BseGI | GGATG | 4 cut(s) | 534, 601, 952, 1117 |
| BseJI | GATNNNNATC | 1 cut(s) | 134 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 331, 453, 454, 466, 764 |
| BseMI | GCAATG | 1 cut(s) | 775 |
| BseMII | CTCAG | 5 cut(s) | 132, 403, 926, 992, 1076 |
| BseNI | ACTGG | 4 cut(s) | 104, 284, 325, 951 |
| BseRI | GAGGAG | 1 cut(s) | 6 |
| BseSI | GKGCMC | 2 cut(s) | 1057, 1092 |
| BseYI | CCCAGC | 1 cut(s) | 609 |
| BsgI | GTGCAG | 4 cut(s) | 905, 1031, 1091, 1248 |
| Bsh1236I | CGCG | 2 cut(s) | 159, 900 |
| BshFI | GGCC | 2 cut(s) | 265, 1079 |
| BshNI | GGYRCC | 1 cut(s) | 745 |
| BshVI | ATCGAT | 1 cut(s) | 87 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1057, 1092, 1127 |
| BsiHKCI | CYCGRG | 1 cut(s) | 639 |
| BsiSI | CCGG | 2 cut(s) | 956, 1268 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 331, 453, 454, 466, 764 |
| BsmAI | GTCTC | 4 cut(s) | 130, 292, 360, 872 |
| BsmI | GAATGC | 1 cut(s) | 619 |
| BsnI | GGCC | 2 cut(s) | 265, 1079 |
| Bso31I | GGTCTC | 1 cut(s) | 360 |
| BsoBI | CYCGRG | 1 cut(s) | 639 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1057, 1092, 1127 |
| Bsp13I | TCCGGA | 1 cut(s) | 955 |
| Bsp143I | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1062 |
| BspACI | CCGC | 3 cut(s) | 159, 253, 370 |
| BspANI | GGCC | 2 cut(s) | 265, 1079 |
| BspCNI | CTCAG | 5 cut(s) | 133, 402, 925, 991, 1075 |
| BspDI | ATCGAT | 1 cut(s) | 87 |
| BspEI | TCCGGA | 1 cut(s) | 955 |
| BspFNI | CGCG | 2 cut(s) | 159, 900 |
| BspLI | GGNNCC | 3 cut(s) | 147, 458, 747 |
| BspMAI | CTGCAG | 1 cut(s) | 52 |
| BspMI | ACCTGC | 3 cut(s) | 777, 1042, 1066 |
| BspPI | GGATC | 2 cut(s) | 477, 539 |
| BspT107I | GGYRCC | 1 cut(s) | 745 |
| BspTNI | GGTCTC | 1 cut(s) | 360 |
| BsrDI | GCAATG | 1 cut(s) | 775 |
| BsrI | ACTGG | 4 cut(s) | 104, 284, 325, 951 |
| BssECI | CCNNGG | 3 cut(s) | 447, 1100, 1266 |
| BssMI | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| BssSI | CACGAG | 1 cut(s) | 1091 |
| BssT1I | CCWWGG | 1 cut(s) | 1100 |
| Bst2BI | CACGAG | 1 cut(s) | 1091 |
| Bst2UI | CCWGG | 3 cut(s) | 411, 449, 1161 |
| Bst4CI | ACNGT | 3 cut(s) | 367, 556, 883 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1018 |
| BstC8I | GCNNGC | 5 cut(s) | 10, 161, 902, 1077, 1362 |
| BstDEI | CTNAG | 5 cut(s) | 141, 389, 912, 978, 1062 |
| BstF5I | GGATG | 4 cut(s) | 534, 601, 952, 1117 |
| BstFNI | CGCG | 2 cut(s) | 159, 900 |
| BstHHI | GCGC | 3 cut(s) | 952, 1006, 1039 |
| BstKTI | GATC | 6 cut(s) | 94, 472, 513, 547, 562, 818 |
| BstMAI | GTCTC | 4 cut(s) | 130, 292, 360, 872 |
| BstMBI | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| BstNI | CCWGG | 3 cut(s) | 411, 449, 1161 |
| BstNSI | RCATGY | 2 cut(s) | 1027, 1136 |
| BstSCI | CCNGG | 4 cut(s) | 409, 447, 1159, 1266 |
| BstSFI | CTRYAG | 1 cut(s) | 48 |
| BstSLI | GKGCMC | 2 cut(s) | 1057, 1092 |
| BstUI | CGCG | 2 cut(s) | 159, 900 |
| BstV2I | GAAGAC | 1 cut(s) | 829 |
| BstX2I | RGATCY | 1 cut(s) | 469 |
| BstYI | RGATCY | 1 cut(s) | 469 |
| Bsu15I | ATCGAT | 1 cut(s) | 87 |
| BsuRI | GGCC | 2 cut(s) | 265, 1079 |
| BsuTUI | ATCGAT | 1 cut(s) | 87 |
| BtsCI | GGATG | 4 cut(s) | 534, 601, 952, 1117 |
| BtsI | GCAGTG | 1 cut(s) | 852 |
| BtsIMutI | CAGTG | 4 cut(s) | 363, 852, 944, 1127 |
| BveI | ACCTGC | 3 cut(s) | 777, 1042, 1066 |
| Cac8I | GCNNGC | 5 cut(s) | 10, 161, 902, 1077, 1362 |
| CaiI | CAGNNNCTG | 1 cut(s) | 881 |
| CfoI | GCGC | 3 cut(s) | 952, 1006, 1039 |
| Cfr13I | GGNCC | 1 cut(s) | 457 |
| ClaI | ATCGAT | 1 cut(s) | 87 |
| CsiI | ACCWGGT | 1 cut(s) | 409 |
| Csp6I | GTAC | 2 cut(s) | 746, 966 |
| CviAII | CATG | 8 cut(s) | 233, 313, 827, 1018, 1024, 1133, 1296, 1357 |
| CviQI | GTAC | 2 cut(s) | 746, 966 |
| DdeI | CTNAG | 5 cut(s) | 141, 389, 912, 978, 1062 |
| DpnI | GATC | 6 cut(s) | 93, 471, 512, 546, 561, 817 |
| DpnII | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| EaeI | YGGCCR | 1 cut(s) | 1077 |
| Eco130I | CCWWGG | 1 cut(s) | 1100 |
| Eco31I | GGTCTC | 1 cut(s) | 360 |
| Eco47I | GGWCC | 1 cut(s) | 457 |
| Eco88I | CYCGRG | 1 cut(s) | 639 |
| EcoRI | GAATTC | 2 cut(s) | 226, 1259 |
| EcoRII | CCWGG | 3 cut(s) | 409, 447, 1159 |
| EcoT14I | CCWWGG | 1 cut(s) | 1100 |
| ErhI | CCWWGG | 1 cut(s) | 1100 |
| FaeI | CATG | 8 cut(s) | 236, 316, 830, 1021, 1027, 1136, 1299, 1360 |
| FatI | CATG | 8 cut(s) | 232, 312, 826, 1017, 1023, 1132, 1295, 1356 |
| FauI | CCCGC | 1 cut(s) | 152 |
| FauNDI | CATATG | 1 cut(s) | 1239 |
| FblI | GTMKAC | 2 cut(s) | 600, 803 |
| FokI | GGATG | 4 cut(s) | 541, 608, 939, 1124 |
| FspBI | CTAG | 2 cut(s) | 110, 582 |
| GlaI | GCGC | 3 cut(s) | 951, 1005, 1038 |
| GsaI | CCCAGC | 1 cut(s) | 613 |
| GsuI | CTGGAG | 1 cut(s) | 87 |
| HaeIII | GGCC | 2 cut(s) | 265, 1079 |
| HapII | CCGG | 2 cut(s) | 956, 1268 |
| HhaI | GCGC | 3 cut(s) | 952, 1006, 1039 |
| Hin1II | CATG | 8 cut(s) | 236, 316, 830, 1021, 1027, 1136, 1299, 1360 |
| Hin6I | GCGC | 3 cut(s) | 950, 1004, 1037 |
| HinP1I | GCGC | 3 cut(s) | 950, 1004, 1037 |
| HincII | GTYRAC | 3 cut(s) | 1279, 1287, 1327 |
| HindII | GTYRAC | 3 cut(s) | 1279, 1287, 1327 |
| HinfI | GANTC | 7 cut(s) | 130, 585, 850, 872, 1232, 1283, 1328 |
| HpaI | GTTAAC | 1 cut(s) | 1279 |
| HpaII | CCGG | 2 cut(s) | 956, 1268 |
| HphI | GGTGA | 2 cut(s) | 1176, 1255 |
| Hpy166II | GTNNAC | 8 cut(s) | 601, 804, 1055, 1090, 1184, 1279, 1287, 1327 |
| Hpy188I | TCNGA | 8 cut(s) | 142, 392, 549, 779, 877, 1129, 1144, 1213 |
| Hpy188III | TCNNGA | 8 cut(s) | 110, 127, 725, 819, 956, 1168, 1199, 1316 |
| Hpy8I | GTNNAC | 8 cut(s) | 601, 804, 1055, 1090, 1184, 1279, 1287, 1327 |
| HpyAV | CCTTC | 4 cut(s) | 40, 164, 774, 1247 |
| HpyCH4III | ACNGT | 3 cut(s) | 367, 556, 883 |
| HpyCH4IV | ACGT | 1 cut(s) | 730 |
| HpyF3I | CTNAG | 5 cut(s) | 141, 389, 912, 978, 1062 |
| HpySE526I | ACGT | 1 cut(s) | 730 |
| Hsp92II | CATG | 8 cut(s) | 236, 316, 830, 1021, 1027, 1136, 1299, 1360 |
| HspAI | GCGC | 3 cut(s) | 950, 1004, 1037 |
| Kpn2I | TCCGGA | 1 cut(s) | 955 |
| KpnI | GGTACC | 1 cut(s) | 749 |
| KspAI | GTTAAC | 1 cut(s) | 1279 |
| Kzo9I | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| LmnI | GCTCC | 3 cut(s) | 255, 490, 958 |
| LweI | GCATC | 2 cut(s) | 519, 961 |
| MabI | ACCWGGT | 1 cut(s) | 409 |
| MaeI | CTAG | 2 cut(s) | 110, 582 |
| MaeII | ACGT | 1 cut(s) | 730 |
| MaeIII | GTNAC | 3 cut(s) | 634, 847, 1233 |
| MalI | GATC | 6 cut(s) | 93, 471, 512, 546, 561, 817 |
| MboI | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| MboII | GAAGA | 2 cut(s) | 834, 1131 |
| MflI | RGATCY | 1 cut(s) | 469 |
| MhlI | GDGCHC | 3 cut(s) | 1057, 1092, 1127 |
| MlsI | TGGCCA | 1 cut(s) | 1079 |
| MluCI | AATT | 6 cut(s) | 226, 436, 627, 656, 1207, 1259 |
| MluNI | TGGCCA | 1 cut(s) | 1079 |
| MlyI | GAGTC | 5 cut(s) | 594, 844, 1241, 1292, 1322 |
| Mox20I | TGGCCA | 1 cut(s) | 1079 |
| MroI | TCCGGA | 1 cut(s) | 955 |
| MroXI | GAANNNNTTC | 1 cut(s) | 684 |
| MscI | TGGCCA | 1 cut(s) | 1079 |
| MseI | TTAA | 4 cut(s) | 239, 374, 395, 1278 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1022, 1085, 1240 |
| Msp20I | TGGCCA | 1 cut(s) | 1079 |
| MspA1I | CMGCKG | 1 cut(s) | 1075 |
| MspI | CCGG | 2 cut(s) | 956, 1268 |
| MspR9I | CCNGG | 4 cut(s) | 411, 449, 1161, 1268 |
| Mva1269I | GAATGC | 1 cut(s) | 619 |
| MvaI | CCWGG | 3 cut(s) | 411, 449, 1161 |
| MvnI | CGCG | 2 cut(s) | 159, 900 |
| NciI | CCSGG | 1 cut(s) | 1268 |
| NdeI | CATATG | 1 cut(s) | 1239 |
| NdeII | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| NlaIII | CATG | 8 cut(s) | 236, 316, 830, 1021, 1027, 1136, 1299, 1360 |
| NlaIV | GGNNCC | 3 cut(s) | 147, 458, 747 |
| NmuCI | GTSAC | 2 cut(s) | 847, 1233 |
| NspI | RCATGY | 2 cut(s) | 1027, 1136 |
| OliI | CACNNNNGTG | 2 cut(s) | 1085, 1240 |
| PaqCI | CACCTGC | 1 cut(s) | 1066 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 637 |
| PctI | GAATGC | 1 cut(s) | 619 |
| PdmI | GAANNNNTTC | 1 cut(s) | 684 |
| PfeI | GAWTC | 2 cut(s) | 130, 872 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 331 |
| PleI | GAGTC | 5 cut(s) | 593, 844, 1240, 1291, 1322 |
| PpsI | GAGTC | 5 cut(s) | 593, 844, 1240, 1291, 1322 |
| PshBI | ATTAAT | 1 cut(s) | 395 |
| Psp6I | CCWGG | 3 cut(s) | 409, 447, 1159 |
| PspFI | CCCAGC | 1 cut(s) | 609 |
| PspGI | CCWGG | 3 cut(s) | 409, 447, 1159 |
| PspN4I | GGNNCC | 3 cut(s) | 147, 458, 747 |
| PspPI | GGNCC | 1 cut(s) | 457 |
| PstI | CTGCAG | 1 cut(s) | 52 |
| PstNI | CAGNNNCTG | 1 cut(s) | 881 |
| PsuI | RGATCY | 1 cut(s) | 469 |
| PvuII | CAGCTG | 1 cut(s) | 1075 |
| RsaI | GTAC | 2 cut(s) | 747, 967 |
| RsaNI | GTAC | 2 cut(s) | 746, 966 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1022, 1085, 1240 |
| SaqAI | TTAA | 4 cut(s) | 239, 374, 395, 1278 |
| Sau3AI | GATC | 6 cut(s) | 91, 469, 510, 544, 559, 815 |
| Sau96I | GGNCC | 1 cut(s) | 457 |
| SchI | GAGTC | 5 cut(s) | 594, 844, 1241, 1292, 1322 |
| ScrFI | CCNGG | 4 cut(s) | 411, 449, 1161, 1268 |
| SduI | GDGCHC | 3 cut(s) | 1057, 1092, 1127 |
| SexAI | ACCWGGT | 1 cut(s) | 409 |
| SfaNI | GCATC | 2 cut(s) | 519, 961 |
| SfcI | CTRYAG | 1 cut(s) | 48 |
| SinI | GGWCC | 1 cut(s) | 457 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1022, 1085, 1240 |
| SmlI | CTYRAG | 2 cut(s) | 1199, 1316 |
| SmoI | CTYRAG | 2 cut(s) | 1199, 1316 |
| Sse9I | AATT | 6 cut(s) | 226, 436, 627, 656, 1207, 1259 |
| SsiI | CCGC | 3 cut(s) | 159, 253, 370 |
| SspMI | CTAG | 2 cut(s) | 110, 582 |
| StyD4I | CCNGG | 4 cut(s) | 409, 447, 1159, 1266 |
| StyI | CCWWGG | 1 cut(s) | 1100 |
| TaaI | ACNGT | 3 cut(s) | 367, 556, 883 |
| TaiI | ACGT | 1 cut(s) | 733 |
| TaqI | TCGA | 5 cut(s) | 87, 128, 480, 797, 814 |
| TasI | AATT | 6 cut(s) | 226, 436, 627, 656, 1207, 1259 |
| TfiI | GAWTC | 2 cut(s) | 130, 872 |
| Tru1I | TTAA | 4 cut(s) | 239, 374, 395, 1278 |
| Tru9I | TTAA | 4 cut(s) | 239, 374, 395, 1278 |
| TscAI | CASTG | 4 cut(s) | 370, 852, 951, 1127 |
| TseFI | GTSAC | 2 cut(s) | 847, 1233 |
| Tsp45I | GTSAC | 2 cut(s) | 847, 1233 |
| TspDTI | ATGAA | 4 cut(s) | 78, 301, 693, 768 |
| TspGWI | ACGGA | 2 cut(s) | 449, 845 |
| TspRI | CASTG | 4 cut(s) | 370, 852, 951, 1127 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 331 |
| VneI | GTGCAC | 2 cut(s) | 1053, 1088 |
| VpaK11BI | GGWCC | 1 cut(s) | 457 |
| VspI | ATTAAT | 1 cut(s) | 395 |
| XapI | RAATTY | 3 cut(s) | 226, 436, 1259 |
| XbaI | TCTAGA | 1 cut(s) | 109 |
| XceI | RCATGY | 2 cut(s) | 1027, 1136 |
| XmiI | GTMKAC | 2 cut(s) | 600, 803 |
| XmnI | GAANNNNTTC | 1 cut(s) | 684 |
| XspI | CTAG | 2 cut(s) | 110, 582 |