Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 242 |
| Acc36I | ACCTGC | 1 cut(s) | 868 |
| AccBSI | CCGCTC | 1 cut(s) | 499 |
| AccI | GTMKAC | 2 cut(s) | 773, 856 |
| AccII | CGCG | 3 cut(s) | 443, 479, 580 |
| AciI | CCGC | 6 cut(s) | 56, 497, 580, 814, 827, 1084 |
| AclWI | GGATC | 1 cut(s) | 536 |
| AcoI | YGGCCR | 3 cut(s) | 187, 260, 715 |
| AcsI | RAATTY | 2 cut(s) | 525, 1208 |
| AcuI | CTGAAG | 1 cut(s) | 159 |
| AcyI | GRCGYC | 1 cut(s) | 239 |
| AfaI | GTAC | 2 cut(s) | 361, 734 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 160, 914, 1112 |
| AgsI | TTSAA | 7 cut(s) | 79, 271, 317, 506, 620, 798, 1139 |
| AjnI | CCWGG | 2 cut(s) | 468, 1048 |
| AleI | CACNNNNGTG | 1 cut(s) | 968 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 823, 855 |
| AluBI | AGCT | 7 cut(s) | 177, 247, 254, 905, 1067, 1235, 1327 |
| AluI | AGCT | 7 cut(s) | 177, 247, 254, 905, 1067, 1235, 1327 |
| Alw21I | GWGCWC | 1 cut(s) | 1300 |
| Alw26I | GTCTC | 7 cut(s) | 127, 289, 337, 575, 786, 1030, 1228 |
| AlwI | GGATC | 1 cut(s) | 536 |
| AoxI | GGCC | 5 cut(s) | 187, 260, 706, 715, 1254 |
| ApeKI | GCWGC | 2 cut(s) | 370, 382 |
| ApoI | RAATTY | 2 cut(s) | 525, 1208 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 458, 490, 932, 964 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 681, 984 |
| AspLEI | GCGC | 3 cut(s) | 7, 445, 580 |
| AspS9I | GGNCC | 3 cut(s) | 279, 466, 839 |
| AsuC2I | CCSGG | 1 cut(s) | 191 |
| AsuHPI | GGTGA | 4 cut(s) | 279, 659, 1187, 1217 |
| AsuII | TTCGAA | 1 cut(s) | 685 |
| AsuNHI | GCTAGC | 1 cut(s) | 1063 |
| AvaII | GGWCC | 3 cut(s) | 279, 466, 839 |
| BalI | TGGCCA | 2 cut(s) | 262, 717 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 894, 926 |
| BauI | CACGAG | 1 cut(s) | 1301 |
| BbsI | GAAGAC | 1 cut(s) | 147 |
| Bbv12I | GWGCWC | 1 cut(s) | 1300 |
| BbvCI | CCTCAGC | 1 cut(s) | 12 |
| BbvI | GCAGC | 2 cut(s) | 357, 369 |
| BccI | CCATC | 1 cut(s) | 726 |
| BciT130I | CCWGG | 2 cut(s) | 470, 1050 |
| BcnI | CCSGG | 1 cut(s) | 191 |
| BcoDI | GTCTC | 7 cut(s) | 127, 289, 337, 575, 786, 1030, 1228 |
| BfaI | CTAG | 5 cut(s) | 546, 897, 1064, 1196, 1275 |
| BfmI | CTRYAG | 1 cut(s) | 427 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BfuAI | ACCTGC | 1 cut(s) | 868 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 714, 723 |
| BglII | AGATCT | 1 cut(s) | 598 |
| BisI | GCNGC | 4 cut(s) | 56, 371, 383, 497 |
| BlsI | GCNGC | 4 cut(s) | 57, 372, 384, 498 |
| Bme1390I | CCNGG | 3 cut(s) | 191, 470, 1050 |
| Bme18I | GGWCC | 3 cut(s) | 279, 466, 839 |
| BmgT120I | GGNCC | 3 cut(s) | 279, 466, 839 |
| BmiI | GGNNCC | 3 cut(s) | 467, 841, 1185 |
| BmrFI | CCNGG | 3 cut(s) | 191, 470, 1050 |
| BmsI | GCATC | 1 cut(s) | 1071 |
| BmtI | GCTAGC | 1 cut(s) | 1067 |
| BpiI | GAAGAC | 1 cut(s) | 147 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1051, 1083 |
| Bpu10I | CCTNAGC | 1 cut(s) | 12 |
| Bpu14I | TTCGAA | 1 cut(s) | 685 |
| BpuMI | CCSGG | 1 cut(s) | 191 |
| BsaHI | GRCGYC | 1 cut(s) | 239 |
| BsaI | GGTCTC | 3 cut(s) | 127, 575, 1030 |
| BsaJI | CCNNGG | 4 cut(s) | 154, 291, 469, 787 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 766, 796 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 160, 914, 1112 |
| Bse118I | RCCGGY | 2 cut(s) | 934, 1186 |
| Bse1I | ACTGG | 2 cut(s) | 1117, 1257 |
| BseBI | CCWGG | 2 cut(s) | 470, 1050 |
| BseDI | CCNNGG | 4 cut(s) | 154, 291, 469, 787 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 160, 914, 1112 |
| BseMII | CTCAG | 4 cut(s) | 26, 138, 620, 791 |
| BseNI | ACTGG | 2 cut(s) | 1117, 1257 |
| BseRI | GAGGAG | 3 cut(s) | 9, 204, 207 |
| BseXI | GCAGC | 2 cut(s) | 357, 369 |
| BseYI | CCCAGC | 1 cut(s) | 864 |
| BsgI | GTGCAG | 1 cut(s) | 1325 |
| Bsh1236I | CGCG | 3 cut(s) | 443, 479, 580 |
| BshFI | GGCC | 5 cut(s) | 189, 262, 708, 717, 1256 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1300 |
| BsiSI | CCGG | 3 cut(s) | 190, 935, 1187 |
| BslFI | GGGAC | 3 cut(s) | 479, 825, 1028 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 160, 914, 1112 |
| BsmAI | GTCTC | 7 cut(s) | 127, 289, 337, 575, 786, 1030, 1228 |
| BsmBI | CGTCTC | 1 cut(s) | 289 |
| BsmFI | GGGAC | 3 cut(s) | 479, 825, 1028 |
| BsnI | GGCC | 5 cut(s) | 189, 262, 708, 717, 1256 |
| Bso31I | GGTCTC | 3 cut(s) | 127, 575, 1030 |
| Bsp119I | TTCGAA | 1 cut(s) | 685 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1300 |
| Bsp143I | GATC | 3 cut(s) | 541, 556, 598 |
| BspACI | CCGC | 6 cut(s) | 56, 497, 580, 814, 827, 1084 |
| BspANI | GGCC | 5 cut(s) | 189, 262, 708, 717, 1256 |
| BspCNI | CTCAG | 4 cut(s) | 25, 137, 619, 790 |
| BspFNI | CGCG | 3 cut(s) | 443, 479, 580 |
| BspLI | GGNNCC | 3 cut(s) | 467, 841, 1185 |
| BspMI | ACCTGC | 1 cut(s) | 868 |
| BspOI | GCTAGC | 1 cut(s) | 1067 |
| BspPI | GGATC | 1 cut(s) | 536 |
| BspT104I | TTCGAA | 1 cut(s) | 685 |
| BspTNI | GGTCTC | 3 cut(s) | 127, 575, 1030 |
| BsrBI | CCGCTC | 1 cut(s) | 499 |
| BsrFI | RCCGGY | 2 cut(s) | 934, 1186 |
| BsrI | ACTGG | 2 cut(s) | 1117, 1257 |
| BssAI | RCCGGY | 2 cut(s) | 934, 1186 |
| BssECI | CCNNGG | 4 cut(s) | 154, 291, 469, 787 |
| BssMI | GATC | 3 cut(s) | 541, 556, 598 |
| BssNI | GRCGYC | 1 cut(s) | 239 |
| BssSI | CACGAG | 1 cut(s) | 1301 |
| BssT1I | CCWWGG | 1 cut(s) | 291 |
| Bst2BI | CACGAG | 1 cut(s) | 1301 |
| Bst2UI | CCWGG | 2 cut(s) | 470, 1050 |
| Bst6I | CTCTTC | 4 cut(s) | 292, 618, 687, 1033 |
| BstACI | GRCGYC | 1 cut(s) | 239 |
| BstBI | TTCGAA | 1 cut(s) | 685 |
| BstC8I | GCNNGC | 8 cut(s) | 812, 825, 889, 932, 936, 1065, 1086, 1188 |
| BstDEI | CTNAG | 5 cut(s) | 12, 124, 606, 777, 1236 |
| BstDSI | CCRYGG | 2 cut(s) | 154, 787 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 912 |
| BstFNI | CGCG | 3 cut(s) | 443, 479, 580 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 3 cut(s) | 7, 445, 580 |
| BstKTI | GATC | 3 cut(s) | 544, 559, 601 |
| BstMAI | GTCTC | 7 cut(s) | 127, 289, 337, 575, 786, 1030, 1228 |
| BstMBI | GATC | 3 cut(s) | 541, 556, 598 |
| BstNI | CCWGG | 2 cut(s) | 470, 1050 |
| BstNSI | RCATGY | 4 cut(s) | 313, 656, 827, 891 |
| BstSCI | CCNGG | 3 cut(s) | 189, 468, 1048 |
| BstSFI | CTRYAG | 1 cut(s) | 427 |
| BstUI | CGCG | 3 cut(s) | 443, 479, 580 |
| BstV1I | GCAGC | 2 cut(s) | 357, 369 |
| BstV2I | GAAGAC | 1 cut(s) | 147 |
| BstX2I | RGATCY | 1 cut(s) | 598 |
| BstYI | RGATCY | 1 cut(s) | 598 |
| BsuRI | GGCC | 5 cut(s) | 189, 262, 708, 717, 1256 |
| BtgI | CCRYGG | 2 cut(s) | 154, 787 |
| BtgZI | GCGATG | 1 cut(s) | 791 |
| BtsIMutI | CAGTG | 1 cut(s) | 57 |
| BveI | ACCTGC | 1 cut(s) | 868 |
| Cac8I | GCNNGC | 8 cut(s) | 812, 825, 889, 932, 936, 1065, 1086, 1188 |
| CfoI | GCGC | 3 cut(s) | 7, 445, 580 |
| Cfr10I | RCCGGY | 2 cut(s) | 934, 1186 |
| Cfr13I | GGNCC | 3 cut(s) | 279, 466, 839 |
| Csp6I | GTAC | 2 cut(s) | 360, 733 |
| CviAII | CATG | 7 cut(s) | 230, 310, 532, 653, 769, 824, 888 |
| CviQI | GTAC | 2 cut(s) | 360, 733 |
| DdeI | CTNAG | 5 cut(s) | 12, 124, 606, 777, 1236 |
| DpnI | GATC | 3 cut(s) | 543, 558, 600 |
| DpnII | GATC | 3 cut(s) | 541, 556, 598 |
| DraI | TTTAAA | 1 cut(s) | 1263 |
| EaeI | YGGCCR | 3 cut(s) | 187, 260, 715 |
| Eam1104I | CTCTTC | 4 cut(s) | 292, 618, 687, 1033 |
| EarI | CTCTTC | 4 cut(s) | 292, 618, 687, 1033 |
| EciI | GGCGGA | 1 cut(s) | 829 |
| Eco130I | CCWWGG | 1 cut(s) | 291 |
| Eco31I | GGTCTC | 3 cut(s) | 127, 575, 1030 |
| Eco47I | GGWCC | 3 cut(s) | 279, 466, 839 |
| Eco57I | CTGAAG | 1 cut(s) | 159 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 912 |
| EcoO109I | RGGNCCY | 1 cut(s) | 466 |
| EcoRI | GAATTC | 2 cut(s) | 525, 1208 |
| EcoRII | CCWGG | 2 cut(s) | 468, 1048 |
| EcoT14I | CCWWGG | 1 cut(s) | 291 |
| ErhI | CCWWGG | 1 cut(s) | 291 |
| Esp3I | CGTCTC | 1 cut(s) | 289 |
| FaeI | CATG | 7 cut(s) | 233, 313, 535, 656, 772, 827, 891 |
| FaqI | GGGAC | 3 cut(s) | 479, 825, 1028 |
| FatI | CATG | 7 cut(s) | 229, 309, 531, 652, 768, 823, 887 |
| FauI | CCCGC | 3 cut(s) | 573, 820, 1077 |
| FblI | GTMKAC | 2 cut(s) | 773, 856 |
| Fnu4HI | GCNGC | 4 cut(s) | 56, 371, 383, 497 |
| Fsp4HI | GCNGC | 4 cut(s) | 56, 371, 383, 497 |
| FspBI | CTAG | 5 cut(s) | 546, 897, 1064, 1196, 1275 |
| GlaI | GCGC | 3 cut(s) | 6, 444, 579 |
| GluI | GCNGC | 4 cut(s) | 56, 371, 383, 497 |
| GsaI | CCCAGC | 1 cut(s) | 868 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HaeIII | GGCC | 5 cut(s) | 189, 262, 708, 717, 1256 |
| HapII | CCGG | 3 cut(s) | 190, 935, 1187 |
| HhaI | GCGC | 3 cut(s) | 7, 445, 580 |
| Hin1I | GRCGYC | 1 cut(s) | 239 |
| Hin1II | CATG | 7 cut(s) | 233, 313, 535, 656, 772, 827, 891 |
| Hin6I | GCGC | 3 cut(s) | 5, 443, 578 |
| HinP1I | GCGC | 3 cut(s) | 5, 443, 578 |
| HincII | GTYRAC | 1 cut(s) | 237 |
| HindII | GTYRAC | 1 cut(s) | 237 |
| HindIII | AAGCTT | 1 cut(s) | 252 |
| HinfI | GANTC | 1 cut(s) | 1091 |
| HpaII | CCGG | 3 cut(s) | 190, 935, 1187 |
| HphI | GGTGA | 4 cut(s) | 279, 659, 1187, 1217 |
| Hpy166II | GTNNAC | 3 cut(s) | 237, 774, 857 |
| Hpy188I | TCNGA | 5 cut(s) | 132, 139, 222, 1018, 1059 |
| Hpy188III | TCNNGA | 2 cut(s) | 107, 998 |
| Hpy8I | GTNNAC | 3 cut(s) | 237, 774, 857 |
| Hpy99I | CGWCG | 2 cut(s) | 89, 1022 |
| HpyAV | CCTTC | 7 cut(s) | 429, 813, 879, 1116, 1196, 1246, 1303 |
| HpyCH4IV | ACGT | 2 cut(s) | 239, 388 |
| HpyCH4V | TGCA | 6 cut(s) | 332, 340, 373, 641, 953, 1306 |
| HpyF3I | CTNAG | 5 cut(s) | 12, 124, 606, 777, 1236 |
| HpySE526I | ACGT | 2 cut(s) | 239, 388 |
| Hsp92I | GRCGYC | 1 cut(s) | 239 |
| Hsp92II | CATG | 7 cut(s) | 233, 313, 535, 656, 772, 827, 891 |
| HspAI | GCGC | 3 cut(s) | 5, 443, 578 |
| KroI | GCCGGC | 2 cut(s) | 934, 1186 |
| KroNI | GCCGGC | 2 cut(s) | 936, 1188 |
| Kzo9I | GATC | 3 cut(s) | 541, 556, 598 |
| LmnI | GCTCC | 5 cut(s) | 163, 487, 910, 1072, 1183 |
| Lsp1109I | GCAGC | 2 cut(s) | 357, 369 |
| LweI | GCATC | 1 cut(s) | 1071 |
| MaeI | CTAG | 5 cut(s) | 546, 897, 1064, 1196, 1275 |
| MaeII | ACGT | 2 cut(s) | 239, 388 |
| MaeIII | GTNAC | 3 cut(s) | 240, 763, 1175 |
| MalI | GATC | 3 cut(s) | 543, 558, 600 |
| MbiI | CCGCTC | 1 cut(s) | 499 |
| MboI | GATC | 3 cut(s) | 541, 556, 598 |
| MboII | GAAGA | 9 cut(s) | 152, 309, 414, 605, 608, 674, 1020, 1158, 1211 |
| MflI | RGATCY | 1 cut(s) | 598 |
| MhlI | GDGCHC | 1 cut(s) | 1300 |
| MlsI | TGGCCA | 2 cut(s) | 262, 717 |
| MluCI | AATT | 6 cut(s) | 274, 317, 525, 623, 630, 1208 |
| MluNI | TGGCCA | 2 cut(s) | 262, 717 |
| MlyI | GAGTC | 1 cut(s) | 1100 |
| MmeI | TCCRAC | 1 cut(s) | 1041 |
| Mox20I | TGGCCA | 2 cut(s) | 262, 717 |
| MroNI | GCCGGC | 2 cut(s) | 934, 1186 |
| MroXI | GAANNNNTTC | 2 cut(s) | 681, 984 |
| MscI | TGGCCA | 2 cut(s) | 262, 717 |
| MseI | TTAA | 5 cut(s) | 249, 921, 957, 1262, 1333 |
| MslI | CAYNNNNRTG | 1 cut(s) | 968 |
| Msp20I | TGGCCA | 2 cut(s) | 262, 717 |
| MspI | CCGG | 3 cut(s) | 190, 935, 1187 |
| MspR9I | CCNGG | 3 cut(s) | 191, 470, 1050 |
| MvaI | CCWGG | 2 cut(s) | 470, 1050 |
| MvnI | CGCG | 3 cut(s) | 443, 479, 580 |
| NaeI | GCCGGC | 2 cut(s) | 936, 1188 |
| NciI | CCSGG | 1 cut(s) | 191 |
| NdeII | GATC | 3 cut(s) | 541, 556, 598 |
| NgoMIV | GCCGGC | 2 cut(s) | 934, 1186 |
| NheI | GCTAGC | 1 cut(s) | 1063 |
| NlaIII | CATG | 7 cut(s) | 233, 313, 535, 656, 772, 827, 891 |
| NlaIV | GGNNCC | 3 cut(s) | 467, 841, 1185 |
| NmeAIII | GCCGAG | 1 cut(s) | 702 |
| NmuCI | GTSAC | 3 cut(s) | 240, 763, 1175 |
| NspI | RCATGY | 4 cut(s) | 313, 656, 827, 891 |
| NspV | TTCGAA | 1 cut(s) | 685 |
| OliI | CACNNNNGTG | 1 cut(s) | 968 |
| PaeI | GCATGC | 2 cut(s) | 827, 891 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 634 |
| PdiI | GCCGGC | 2 cut(s) | 936, 1188 |
| PdmI | GAANNNNTTC | 2 cut(s) | 681, 984 |
| PflFI | GACNNNGTC | 2 cut(s) | 769, 1022 |
| PkrI | GCNGC | 4 cut(s) | 57, 372, 384, 498 |
| PleI | GAGTC | 1 cut(s) | 1099 |
| PpsI | GAGTC | 1 cut(s) | 1099 |
| PpuMI | RGGWCCY | 1 cut(s) | 466 |
| Psp5II | RGGWCCY | 1 cut(s) | 466 |
| Psp6I | CCWGG | 2 cut(s) | 468, 1048 |
| PspFI | CCCAGC | 1 cut(s) | 864 |
| PspGI | CCWGG | 2 cut(s) | 468, 1048 |
| PspN4I | GGNNCC | 3 cut(s) | 467, 841, 1185 |
| PspPI | GGNCC | 3 cut(s) | 279, 466, 839 |
| PspPPI | RGGWCCY | 1 cut(s) | 466 |
| PsuI | RGATCY | 1 cut(s) | 598 |
| PsyI | GACNNNGTC | 2 cut(s) | 769, 1022 |
| RsaI | GTAC | 2 cut(s) | 361, 734 |
| RsaNI | GTAC | 2 cut(s) | 360, 733 |
| RseI | CAYNNNNRTG | 1 cut(s) | 968 |
| SaqAI | TTAA | 5 cut(s) | 249, 921, 957, 1262, 1333 |
| SatI | GCNGC | 4 cut(s) | 56, 371, 383, 497 |
| Sau3AI | GATC | 3 cut(s) | 541, 556, 598 |
| Sau96I | GGNCC | 3 cut(s) | 279, 466, 839 |
| SchI | GAGTC | 1 cut(s) | 1100 |
| ScrFI | CCNGG | 3 cut(s) | 191, 470, 1050 |
| SduI | GDGCHC | 1 cut(s) | 1300 |
| SfaNI | GCATC | 1 cut(s) | 1071 |
| SfcI | CTRYAG | 1 cut(s) | 427 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 714 |
| SfuI | TTCGAA | 1 cut(s) | 685 |
| SinI | GGWCC | 3 cut(s) | 279, 466, 839 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 968 |
| SphI | GCATGC | 2 cut(s) | 827, 891 |
| Sse9I | AATT | 6 cut(s) | 274, 317, 525, 623, 630, 1208 |
| SsiI | CCGC | 6 cut(s) | 56, 497, 580, 814, 827, 1084 |
| SspMI | CTAG | 5 cut(s) | 546, 897, 1064, 1196, 1275 |
| StyD4I | CCNGG | 3 cut(s) | 189, 468, 1048 |
| StyI | CCWWGG | 1 cut(s) | 291 |
| TaiI | ACGT | 2 cut(s) | 242, 391 |
| TaqI | TCGA | 3 cut(s) | 84, 457, 685 |
| TaqII | GACCGA | 1 cut(s) | 557 |
| TasI | AATT | 6 cut(s) | 274, 317, 525, 623, 630, 1208 |
| TatI | WGTACW | 1 cut(s) | 732 |
| TauI | GCSGC | 2 cut(s) | 58, 499 |
| Tru1I | TTAA | 5 cut(s) | 249, 921, 957, 1262, 1333 |
| Tru9I | TTAA | 5 cut(s) | 249, 921, 957, 1262, 1333 |
| TscAI | CASTG | 1 cut(s) | 57 |
| TseFI | GTSAC | 3 cut(s) | 240, 763, 1175 |
| TseI | GCWGC | 2 cut(s) | 370, 382 |
| Tsp45I | GTSAC | 3 cut(s) | 240, 763, 1175 |
| TspDTI | ATGAA | 4 cut(s) | 548, 690, 774, 1020 |
| TspGWI | ACGGA | 3 cut(s) | 143, 271, 316 |
| TspRI | CASTG | 1 cut(s) | 57 |
| Tth111I | GACNNNGTC | 2 cut(s) | 769, 1022 |
| VpaK11BI | GGWCC | 3 cut(s) | 279, 466, 839 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 912 |
| XapI | RAATTY | 2 cut(s) | 525, 1208 |
| XceI | RCATGY | 4 cut(s) | 313, 656, 827, 891 |
| XmiI | GTMKAC | 2 cut(s) | 773, 856 |
| XmnI | GAANNNNTTC | 2 cut(s) | 681, 984 |
| XspI | CTAG | 5 cut(s) | 546, 897, 1064, 1196, 1275 |
| ZraI | GACGTC | 1 cut(s) | 240 |