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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 312 |
| AciI | CCGC | 2 cut(s) | 37, 152 |
| AfaI | GTAC | 1 cut(s) | 30 |
| AfeI | AGCGCT | 1 cut(s) | 249 |
| AflIII | ACRYGT | 1 cut(s) | 478 |
| AgsI | TTSAA | 7 cut(s) | 20, 172, 180, 220, 485, 530, 648 |
| AluBI | AGCT | 4 cut(s) | 265, 443, 502, 562 |
| AluI | AGCT | 4 cut(s) | 265, 443, 502, 562 |
| Alw26I | GTCTC | 2 cut(s) | 478, 509 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 518 |
| Aor51HI | AGCGCT | 1 cut(s) | 249 |
| AoxI | GGCC | 2 cut(s) | 47, 635 |
| ApeKI | GCWGC | 2 cut(s) | 11, 250 |
| AspLEI | GCGC | 2 cut(s) | 85, 250 |
| AspS9I | GGNCC | 2 cut(s) | 635, 668 |
| AsuC2I | CCSGG | 1 cut(s) | 207 |
| AsuHPI | GGTGA | 1 cut(s) | 109 |
| AvaII | GGWCC | 1 cut(s) | 668 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 12, 44 |
| BbvI | GCAGC | 1 cut(s) | 237 |
| BciVI | GTATCC | 2 cut(s) | 203, 562 |
| BclI | TGATCA | 2 cut(s) | 99, 450 |
| BcnI | CCSGG | 1 cut(s) | 207 |
| BcoDI | GTCTC | 2 cut(s) | 478, 509 |
| BfaI | CTAG | 1 cut(s) | 697 |
| BfoI | RGCGCY | 2 cut(s) | 86, 251 |
| BfuI | GTATCC | 2 cut(s) | 203, 562 |
| BglII | AGATCT | 1 cut(s) | 289 |
| BisI | GCNGC | 3 cut(s) | 12, 38, 251 |
| BlsI | GCNGC | 3 cut(s) | 13, 39, 252 |
| Bme1390I | CCNGG | 1 cut(s) | 207 |
| Bme18I | GGWCC | 1 cut(s) | 668 |
| BmgT120I | GGNCC | 2 cut(s) | 635, 668 |
| BmrFI | CCNGG | 1 cut(s) | 207 |
| BmsI | GCATC | 3 cut(s) | 409, 604, 626 |
| BpmI | CTGGAG | 2 cut(s) | 123, 495 |
| BpuEI | CTTGAG | 1 cut(s) | 411 |
| BpuMI | CCSGG | 1 cut(s) | 207 |
| BsaJI | CCNNGG | 1 cut(s) | 555 |
| Bse1I | ACTGG | 1 cut(s) | 140 |
| BseDI | CCNNGG | 1 cut(s) | 555 |
| BseGI | GGATG | 1 cut(s) | 682 |
| BseMII | CTCAG | 1 cut(s) | 35 |
| BseNI | ACTGG | 1 cut(s) | 140 |
| BseRI | GAGGAG | 1 cut(s) | 126 |
| BseXI | GCAGC | 1 cut(s) | 237 |
| BshFI | GGCC | 2 cut(s) | 49, 637 |
| BsiSI | CCGG | 1 cut(s) | 206 |
| BsmAI | GTCTC | 2 cut(s) | 478, 509 |
| BsnI | GGCC | 2 cut(s) | 49, 637 |
| Bsp143I | GATC | 3 cut(s) | 99, 289, 450 |
| BspACI | CCGC | 2 cut(s) | 37, 152 |
| BspANI | GGCC | 2 cut(s) | 49, 637 |
| BspCNI | CTCAG | 1 cut(s) | 36 |
| BsrI | ACTGG | 1 cut(s) | 140 |
| BssECI | CCNNGG | 1 cut(s) | 555 |
| BssMI | GATC | 3 cut(s) | 99, 289, 450 |
| BssNAI | GTATAC | 1 cut(s) | 313 |
| Bst1107I | GTATAC | 1 cut(s) | 313 |
| Bst4CI | ACNGT | 5 cut(s) | 64, 97, 176, 520, 658 |
| Bst6I | CTCTTC | 2 cut(s) | 371, 393 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 266 |
| BstC8I | GCNNGC | 4 cut(s) | 186, 267, 504, 603 |
| BstDEI | CTNAG | 3 cut(s) | 44, 107, 332 |
| BstDSI | CCRYGG | 1 cut(s) | 555 |
| BstF5I | GGATG | 1 cut(s) | 682 |
| BstH2I | RGCGCY | 2 cut(s) | 86, 251 |
| BstHHI | GCGC | 2 cut(s) | 85, 250 |
| BstKTI | GATC | 3 cut(s) | 102, 292, 453 |
| BstMAI | GTCTC | 2 cut(s) | 478, 509 |
| BstMBI | GATC | 3 cut(s) | 99, 289, 450 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 46, 80, 266, 271, 428 |
| BstNSI | RCATGY | 1 cut(s) | 482 |
| BstSCI | CCNGG | 1 cut(s) | 205 |
| BstV1I | GCAGC | 1 cut(s) | 237 |
| BstX2I | RGATCY | 1 cut(s) | 289 |
| BstYI | RGATCY | 1 cut(s) | 289 |
| BstZ17I | GTATAC | 1 cut(s) | 313 |
| BsuI | GTATCC | 2 cut(s) | 203, 562 |
| BsuRI | GGCC | 2 cut(s) | 49, 637 |
| BtgI | CCRYGG | 1 cut(s) | 555 |
| BtsCI | GGATG | 1 cut(s) | 682 |
| BtsI | GCAGTG | 1 cut(s) | 269 |
| BtsIMutI | CAGTG | 2 cut(s) | 269, 588 |
| Cac8I | GCNNGC | 4 cut(s) | 186, 267, 504, 603 |
| CaiI | CAGNNNCTG | 1 cut(s) | 518 |
| CfoI | GCGC | 2 cut(s) | 85, 250 |
| Cfr13I | GGNCC | 2 cut(s) | 635, 668 |
| Csp6I | GTAC | 1 cut(s) | 29 |
| CviAII | CATG | 3 cut(s) | 8, 479, 687 |
| CviQI | GTAC | 1 cut(s) | 29 |
| DdeI | CTNAG | 3 cut(s) | 44, 107, 332 |
| DpnI | GATC | 3 cut(s) | 101, 291, 452 |
| DpnII | GATC | 3 cut(s) | 99, 289, 450 |
| Eam1104I | CTCTTC | 2 cut(s) | 371, 393 |
| EarI | CTCTTC | 2 cut(s) | 371, 393 |
| Eco47I | GGWCC | 1 cut(s) | 668 |
| Eco47III | AGCGCT | 1 cut(s) | 249 |
| EcoT22I | ATGCAT | 1 cut(s) | 619 |
| FaeI | CATG | 3 cut(s) | 11, 482, 690 |
| FaiI | YATR | 9 cut(s) | 9, 257, 277, 313, 338, 344, 414, 480, 688 |
| FatI | CATG | 3 cut(s) | 7, 478, 686 |
| FbaI | TGATCA | 2 cut(s) | 99, 450 |
| FblI | GTMKAC | 1 cut(s) | 312 |
| Fnu4HI | GCNGC | 3 cut(s) | 12, 38, 251 |
| FokI | GGATG | 1 cut(s) | 669 |
| Fsp4HI | GCNGC | 3 cut(s) | 12, 38, 251 |
| FspBI | CTAG | 1 cut(s) | 697 |
| GlaI | GCGC | 2 cut(s) | 84, 249 |
| GluI | GCNGC | 3 cut(s) | 12, 38, 251 |
| GsuI | CTGGAG | 2 cut(s) | 123, 495 |
| HaeII | RGCGCY | 2 cut(s) | 86, 251 |
| HaeIII | GGCC | 2 cut(s) | 49, 637 |
| HapII | CCGG | 1 cut(s) | 206 |
| HhaI | GCGC | 2 cut(s) | 85, 250 |
| Hin1II | CATG | 3 cut(s) | 11, 482, 690 |
| Hin6I | GCGC | 2 cut(s) | 83, 248 |
| HinP1I | GCGC | 2 cut(s) | 83, 248 |
| HindIII | AAGCTT | 1 cut(s) | 263 |
| HinfI | GANTC | 2 cut(s) | 238, 644 |
| HpaII | CCGG | 1 cut(s) | 206 |
| HphI | GGTGA | 1 cut(s) | 109 |
| Hpy166II | GTNNAC | 3 cut(s) | 313, 523, 677 |
| Hpy188I | TCNGA | 5 cut(s) | 93, 297, 353, 375, 397 |
| Hpy188III | TCNNGA | 1 cut(s) | 512 |
| Hpy8I | GTNNAC | 3 cut(s) | 313, 523, 677 |
| Hpy99I | CGWCG | 1 cut(s) | 97 |
| HpyCH4III | ACNGT | 5 cut(s) | 64, 97, 176, 520, 658 |
| HpyCH4V | TGCA | 3 cut(s) | 269, 305, 617 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 46, 80, 266, 271, 428 |
| HpyF3I | CTNAG | 3 cut(s) | 44, 107, 332 |
| Hsp92II | CATG | 3 cut(s) | 11, 482, 690 |
| HspAI | GCGC | 2 cut(s) | 83, 248 |
| Ksp22I | TGATCA | 2 cut(s) | 99, 450 |
| Kzo9I | GATC | 3 cut(s) | 99, 289, 450 |
| LmnI | GCTCC | 1 cut(s) | 559 |
| LpnPI | CCDG | 6 cut(s) | 153, 202, 219, 504, 525, 651 |
| Lsp1109I | GCAGC | 1 cut(s) | 237 |
| LweI | GCATC | 3 cut(s) | 409, 604, 626 |
| MaeI | CTAG | 1 cut(s) | 697 |
| MaeIII | GTNAC | 3 cut(s) | 382, 404, 652 |
| MalI | GATC | 3 cut(s) | 101, 291, 452 |
| MboI | GATC | 3 cut(s) | 99, 289, 450 |
| MboII | GAAGA | 5 cut(s) | 284, 310, 388, 410, 542 |
| MfeI | CAATTG | 2 cut(s) | 223, 306 |
| MflI | RGATCY | 1 cut(s) | 289 |
| MluCI | AATT | 3 cut(s) | 223, 306, 495 |
| MmeI | TCCRAC | 2 cut(s) | 116, 320 |
| MnlI | CCTC | 6 cut(s) | 27, 39, 99, 144, 147, 541 |
| Mph1103I | ATGCAT | 1 cut(s) | 619 |
| MseI | TTAA | 1 cut(s) | 567 |
| MslI | CAYNNNNRTG | 2 cut(s) | 417, 685 |
| MspI | CCGG | 1 cut(s) | 206 |
| MspR9I | CCNGG | 1 cut(s) | 207 |
| MunI | CAATTG | 2 cut(s) | 223, 306 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 46, 80, 266, 271, 428 |
| NciI | CCSGG | 1 cut(s) | 207 |
| NdeII | GATC | 3 cut(s) | 99, 289, 450 |
| NlaIII | CATG | 3 cut(s) | 11, 482, 690 |
| NmuCI | GTSAC | 1 cut(s) | 652 |
| NsiI | ATGCAT | 1 cut(s) | 619 |
| NspI | RCATGY | 1 cut(s) | 482 |
| PciI | ACATGT | 1 cut(s) | 478 |
| PfeI | GAWTC | 2 cut(s) | 238, 644 |
| PkrI | GCNGC | 3 cut(s) | 13, 39, 252 |
| PscI | ACATGT | 1 cut(s) | 478 |
| PspPI | GGNCC | 2 cut(s) | 635, 668 |
| PstNI | CAGNNNCTG | 1 cut(s) | 518 |
| PsuI | RGATCY | 1 cut(s) | 289 |
| RsaI | GTAC | 1 cut(s) | 30 |
| RsaNI | GTAC | 1 cut(s) | 29 |
| RseI | CAYNNNNRTG | 2 cut(s) | 417, 685 |
| SaqAI | TTAA | 1 cut(s) | 567 |
| SatI | GCNGC | 3 cut(s) | 12, 38, 251 |
| Sau3AI | GATC | 3 cut(s) | 99, 289, 450 |
| Sau96I | GGNCC | 2 cut(s) | 635, 668 |
| ScrFI | CCNGG | 1 cut(s) | 207 |
| SetI | ASST | 7 cut(s) | 91, 136, 267, 445, 504, 552, 564 |
| SfaNI | GCATC | 3 cut(s) | 409, 604, 626 |
| SinI | GGWCC | 1 cut(s) | 668 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 417, 685 |
| SmlI | CTYRAG | 1 cut(s) | 426 |
| SmoI | CTYRAG | 1 cut(s) | 426 |
| Sse9I | AATT | 3 cut(s) | 223, 306, 495 |
| SsiI | CCGC | 2 cut(s) | 37, 152 |
| SspI | AATATT | 2 cut(s) | 168, 469 |
| SspMI | CTAG | 1 cut(s) | 697 |
| StyD4I | CCNGG | 1 cut(s) | 205 |
| TaaI | ACNGT | 5 cut(s) | 64, 97, 176, 520, 658 |
| TaqI | TCGA | 1 cut(s) | 693 |
| TasI | AATT | 3 cut(s) | 223, 306, 495 |
| TauI | GCSGC | 1 cut(s) | 40 |
| TfiI | GAWTC | 2 cut(s) | 238, 644 |
| Tru1I | TTAA | 1 cut(s) | 567 |
| Tru9I | TTAA | 1 cut(s) | 567 |
| TscAI | CASTG | 2 cut(s) | 276, 595 |
| TseFI | GTSAC | 1 cut(s) | 652 |
| TseI | GCWGC | 2 cut(s) | 11, 250 |
| Tsp45I | GTSAC | 1 cut(s) | 652 |
| TspGWI | ACGGA | 2 cut(s) | 46, 572 |
| TspRI | CASTG | 2 cut(s) | 276, 595 |
| VpaK11BI | GGWCC | 1 cut(s) | 668 |
| XceI | RCATGY | 1 cut(s) | 482 |
| XmiI | GTMKAC | 1 cut(s) | 312 |
| XspI | CTAG | 1 cut(s) | 697 |
| Zsp2I | ATGCAT | 1 cut(s) | 619 |