Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 52 |
| AatII | GACGTC | 1 cut(s) | 50 |
| Acc16I | TGCGCA | 1 cut(s) | 200 |
| AccBSI | CCGCTC | 1 cut(s) | 147 |
| AccI | GTMKAC | 1 cut(s) | 353 |
| AccII | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| AciI | CCGC | 4 cut(s) | 5, 32, 147, 150 |
| AclWI | GGATC | 1 cut(s) | 788 |
| AcsI | RAATTY | 2 cut(s) | 513, 879 |
| AcyI | GRCGYC | 1 cut(s) | 47 |
| AfaI | GTAC | 2 cut(s) | 403, 875 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 149, 714, 746 |
| AgsI | TTSAA | 4 cut(s) | 251, 512, 635, 801 |
| AjnI | CCWGG | 1 cut(s) | 14 |
| AleI | CACNNNNGTG | 1 cut(s) | 309 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 664, 696 |
| AluBI | AGCT | 5 cut(s) | 189, 275, 408, 605, 703 |
| AluI | AGCT | 5 cut(s) | 189, 275, 408, 605, 703 |
| Alw26I | GTCTC | 1 cut(s) | 641 |
| AlwI | GGATC | 1 cut(s) | 788 |
| AoxI | GGCC | 1 cut(s) | 174 |
| ApeKI | GCWGC | 1 cut(s) | 662 |
| ApoI | RAATTY | 2 cut(s) | 513, 879 |
| AspLEI | GCGC | 3 cut(s) | 25, 27, 201 |
| AsuHPI | GGTGA | 3 cut(s) | 88, 469, 656 |
| BauI | CACGAG | 1 cut(s) | 259 |
| BbvI | GCAGC | 1 cut(s) | 649 |
| BccI | CCATC | 1 cut(s) | 739 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 55, 89 |
| BciT130I | CCWGG | 1 cut(s) | 16 |
| BcoDI | GTCTC | 1 cut(s) | 641 |
| BfaI | CTAG | 3 cut(s) | 329, 426, 582 |
| BfmI | CTRYAG | 2 cut(s) | 489, 606 |
| BisI | GCNGC | 3 cut(s) | 148, 151, 663 |
| BlsI | GCNGC | 3 cut(s) | 149, 152, 664 |
| Bme1390I | CCNGG | 1 cut(s) | 16 |
| BmiI | GGNNCC | 2 cut(s) | 154, 267 |
| BmrFI | CCNGG | 1 cut(s) | 16 |
| BmsI | GCATC | 3 cut(s) | 742, 772, 806 |
| BpmI | CTGGAG | 1 cut(s) | 787 |
| BsaHI | GRCGYC | 1 cut(s) | 47 |
| BsaJI | CCNNGG | 4 cut(s) | 14, 124, 171, 689 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 149, 714, 746 |
| Bse1I | ACTGG | 1 cut(s) | 379 |
| BseBI | CCWGG | 1 cut(s) | 16 |
| BseDI | CCNNGG | 4 cut(s) | 14, 124, 171, 689 |
| BseGI | GGATG | 3 cut(s) | 574, 703, 821 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 149, 714, 746 |
| BseMII | CTCAG | 2 cut(s) | 199, 639 |
| BseNI | ACTGG | 1 cut(s) | 379 |
| BsePI | GCGCGC | 1 cut(s) | 23 |
| BseRI | GAGGAG | 3 cut(s) | 107, 145, 226 |
| BseXI | GCAGC | 1 cut(s) | 649 |
| Bsh1236I | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| BshFI | GGCC | 1 cut(s) | 176 |
| BsiSI | CCGG | 1 cut(s) | 159 |
| BslFI | GGGAC | 1 cut(s) | 519 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 149, 714, 746 |
| BsmAI | GTCTC | 1 cut(s) | 641 |
| BsmFI | GGGAC | 1 cut(s) | 519 |
| BsnI | GGCC | 1 cut(s) | 176 |
| Bsp143I | GATC | 2 cut(s) | 181, 793 |
| BspACI | CCGC | 4 cut(s) | 5, 32, 147, 150 |
| BspANI | GGCC | 1 cut(s) | 176 |
| BspCNI | CTCAG | 2 cut(s) | 198, 638 |
| BspFNI | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| BspLI | GGNNCC | 2 cut(s) | 154, 267 |
| BspPI | GGATC | 1 cut(s) | 788 |
| BsrBI | CCGCTC | 1 cut(s) | 147 |
| BsrI | ACTGG | 1 cut(s) | 379 |
| BssECI | CCNNGG | 4 cut(s) | 14, 124, 171, 689 |
| BssHII | GCGCGC | 1 cut(s) | 23 |
| BssMI | GATC | 2 cut(s) | 181, 793 |
| BssNI | GRCGYC | 1 cut(s) | 47 |
| BssSI | CACGAG | 1 cut(s) | 259 |
| BssT1I | CCWWGG | 2 cut(s) | 171, 689 |
| Bst2BI | CACGAG | 1 cut(s) | 259 |
| Bst2UI | CCWGG | 1 cut(s) | 16 |
| Bst4CI | ACNGT | 4 cut(s) | 358, 368, 610, 652 |
| BstACI | GRCGYC | 1 cut(s) | 47 |
| BstC8I | GCNNGC | 2 cut(s) | 21, 25 |
| BstDEI | CTNAG | 3 cut(s) | 185, 625, 741 |
| BstF5I | GGATG | 3 cut(s) | 574, 703, 821 |
| BstFNI | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| BstHHI | GCGC | 3 cut(s) | 25, 27, 201 |
| BstKTI | GATC | 2 cut(s) | 184, 796 |
| BstMAI | GTCTC | 1 cut(s) | 641 |
| BstMBI | GATC | 2 cut(s) | 181, 793 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 29, 82, 159, 390 |
| BstNI | CCWGG | 1 cut(s) | 16 |
| BstSCI | CCNGG | 1 cut(s) | 14 |
| BstSFI | CTRYAG | 2 cut(s) | 489, 606 |
| BstUI | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| BstV1I | GCAGC | 1 cut(s) | 649 |
| BstX2I | RGATCY | 1 cut(s) | 793 |
| BstYI | RGATCY | 1 cut(s) | 793 |
| BsuRI | GGCC | 1 cut(s) | 176 |
| BtsCI | GGATG | 3 cut(s) | 574, 703, 821 |
| BtsI | GCAGTG | 1 cut(s) | 248 |
| BtsIMutI | CAGTG | 2 cut(s) | 248, 373 |
| Cac8I | GCNNGC | 2 cut(s) | 21, 25 |
| CfoI | GCGC | 3 cut(s) | 25, 27, 201 |
| CseI | GACGC | 1 cut(s) | 16 |
| Csp6I | GTAC | 2 cut(s) | 402, 874 |
| CviAII | CATG | 3 cut(s) | 91, 772, 809 |
| CviQI | GTAC | 2 cut(s) | 402, 874 |
| DdeI | CTNAG | 3 cut(s) | 185, 625, 741 |
| DpnI | GATC | 2 cut(s) | 183, 795 |
| DpnII | GATC | 2 cut(s) | 181, 793 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 52 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 52 |
| Eco130I | CCWWGG | 2 cut(s) | 171, 689 |
| EcoRII | CCWGG | 1 cut(s) | 14 |
| EcoT14I | CCWWGG | 2 cut(s) | 171, 689 |
| ErhI | CCWWGG | 2 cut(s) | 171, 689 |
| FaeI | CATG | 3 cut(s) | 94, 775, 812 |
| FaiI | YATR | 9 cut(s) | 92, 344, 525, 543, 576, 602, 677, 773, 810 |
| FaqI | GGGAC | 1 cut(s) | 519 |
| FatI | CATG | 3 cut(s) | 90, 771, 808 |
| FauI | CCCGC | 1 cut(s) | 25 |
| FblI | GTMKAC | 1 cut(s) | 353 |
| Fnu4HI | GCNGC | 3 cut(s) | 148, 151, 663 |
| FokI | GGATG | 3 cut(s) | 581, 710, 828 |
| Fsp4HI | GCNGC | 3 cut(s) | 148, 151, 663 |
| FspBI | CTAG | 3 cut(s) | 329, 426, 582 |
| FspI | TGCGCA | 1 cut(s) | 200 |
| GlaI | GCGC | 3 cut(s) | 24, 26, 200 |
| GluI | GCNGC | 3 cut(s) | 148, 151, 663 |
| GsuI | CTGGAG | 1 cut(s) | 787 |
| HaeIII | GGCC | 1 cut(s) | 176 |
| HapII | CCGG | 1 cut(s) | 159 |
| HgaI | GACGC | 1 cut(s) | 16 |
| HhaI | GCGC | 3 cut(s) | 25, 27, 201 |
| Hin1I | GRCGYC | 1 cut(s) | 47 |
| Hin1II | CATG | 3 cut(s) | 94, 775, 812 |
| Hin6I | GCGC | 3 cut(s) | 23, 25, 199 |
| HinP1I | GCGC | 3 cut(s) | 23, 25, 199 |
| HincII | GTYRAC | 3 cut(s) | 354, 364, 596 |
| HindII | GTYRAC | 3 cut(s) | 354, 364, 596 |
| HinfI | GANTC | 7 cut(s) | 67, 129, 164, 193, 214, 775, 859 |
| HpaII | CCGG | 1 cut(s) | 159 |
| HphI | GGTGA | 3 cut(s) | 88, 469, 656 |
| Hpy166II | GTNNAC | 5 cut(s) | 96, 354, 364, 596, 868 |
| Hpy188I | TCNGA | 6 cut(s) | 128, 144, 533, 615, 628, 709 |
| Hpy188III | TCNNGA | 6 cut(s) | 295, 411, 448, 566, 766, 779 |
| Hpy8I | GTNNAC | 5 cut(s) | 96, 354, 364, 596, 868 |
| Hpy99I | CGWCG | 4 cut(s) | 32, 49, 52, 55 |
| HpyAV | CCTTC | 3 cut(s) | 109, 632, 807 |
| HpyCH4III | ACNGT | 4 cut(s) | 358, 368, 610, 652 |
| HpyCH4IV | ACGT | 2 cut(s) | 47, 598 |
| HpyCH4V | TGCA | 2 cut(s) | 785, 808 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 29, 82, 159, 390 |
| HpyF3I | CTNAG | 3 cut(s) | 185, 625, 741 |
| HpySE526I | ACGT | 2 cut(s) | 47, 598 |
| Hsp92I | GRCGYC | 1 cut(s) | 47 |
| Hsp92II | CATG | 3 cut(s) | 94, 775, 812 |
| HspAI | GCGC | 3 cut(s) | 23, 25, 199 |
| Kzo9I | GATC | 2 cut(s) | 181, 793 |
| LmnI | GCTCC | 4 cut(s) | 158, 238, 265, 768 |
| Lsp1109I | GCAGC | 1 cut(s) | 649 |
| LweI | GCATC | 3 cut(s) | 742, 772, 806 |
| MaeI | CTAG | 3 cut(s) | 329, 426, 582 |
| MaeII | ACGT | 2 cut(s) | 47, 598 |
| MalI | GATC | 2 cut(s) | 183, 795 |
| MauBI | CGCGCGCG | 1 cut(s) | 23 |
| MbiI | CCGCTC | 1 cut(s) | 147 |
| MboI | GATC | 2 cut(s) | 181, 793 |
| MboII | GAAGA | 1 cut(s) | 647 |
| MflI | RGATCY | 1 cut(s) | 793 |
| MluCI | AATT | 4 cut(s) | 289, 472, 513, 879 |
| MlyI | GAGTC | 3 cut(s) | 61, 158, 223 |
| MmeI | TCCRAC | 1 cut(s) | 511 |
| MseI | TTAA | 2 cut(s) | 9, 735 |
| MslI | CAYNNNNRTG | 1 cut(s) | 309 |
| MspI | CCGG | 1 cut(s) | 159 |
| MspR9I | CCNGG | 1 cut(s) | 16 |
| MvaI | CCWGG | 1 cut(s) | 16 |
| MvnI | CGCG | 4 cut(s) | 23, 25, 27, 32 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 29, 82, 159, 390 |
| NdeII | GATC | 2 cut(s) | 181, 793 |
| NlaIII | CATG | 3 cut(s) | 94, 775, 812 |
| NlaIV | GGNNCC | 2 cut(s) | 154, 267 |
| NmeAIII | GCCGAG | 1 cut(s) | 232 |
| NsbI | TGCGCA | 1 cut(s) | 200 |
| OliI | CACNNNNGTG | 1 cut(s) | 309 |
| PauI | GCGCGC | 1 cut(s) | 23 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 50, 56 |
| PfeI | GAWTC | 4 cut(s) | 129, 193, 775, 859 |
| PflFI | GACNNNGTC | 1 cut(s) | 49 |
| PkrI | GCNGC | 3 cut(s) | 149, 152, 664 |
| PleI | GAGTC | 3 cut(s) | 61, 158, 222 |
| PpsI | GAGTC | 3 cut(s) | 61, 158, 222 |
| Psp6I | CCWGG | 1 cut(s) | 14 |
| PspGI | CCWGG | 1 cut(s) | 14 |
| PspN4I | GGNNCC | 2 cut(s) | 154, 267 |
| PsuI | RGATCY | 1 cut(s) | 793 |
| PsyI | GACNNNGTC | 1 cut(s) | 49 |
| PteI | GCGCGC | 1 cut(s) | 23 |
| RsaI | GTAC | 2 cut(s) | 403, 875 |
| RsaNI | GTAC | 2 cut(s) | 402, 874 |
| RseI | CAYNNNNRTG | 1 cut(s) | 309 |
| SalI | GTCGAC | 1 cut(s) | 352 |
| SaqAI | TTAA | 2 cut(s) | 9, 735 |
| SatI | GCNGC | 3 cut(s) | 148, 151, 663 |
| Sau3AI | GATC | 2 cut(s) | 181, 793 |
| SchI | GAGTC | 3 cut(s) | 61, 158, 223 |
| ScrFI | CCNGG | 1 cut(s) | 16 |
| SfaNI | GCATC | 3 cut(s) | 742, 772, 806 |
| SfcI | CTRYAG | 2 cut(s) | 489, 606 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 309 |
| Sse9I | AATT | 4 cut(s) | 289, 472, 513, 879 |
| SsiI | CCGC | 4 cut(s) | 5, 32, 147, 150 |
| SspMI | CTAG | 3 cut(s) | 329, 426, 582 |
| StyD4I | CCNGG | 1 cut(s) | 14 |
| StyI | CCWWGG | 2 cut(s) | 171, 689 |
| TaaI | ACNGT | 4 cut(s) | 358, 368, 610, 652 |
| TaiI | ACGT | 2 cut(s) | 50, 601 |
| TaqI | TCGA | 3 cut(s) | 65, 191, 353 |
| TasI | AATT | 4 cut(s) | 289, 472, 513, 879 |
| TatI | WGTACW | 1 cut(s) | 873 |
| TauI | GCSGC | 2 cut(s) | 150, 153 |
| TfiI | GAWTC | 4 cut(s) | 129, 193, 775, 859 |
| Tru1I | TTAA | 2 cut(s) | 9, 735 |
| Tru9I | TTAA | 2 cut(s) | 9, 735 |
| TscAI | CASTG | 2 cut(s) | 248, 373 |
| TseI | GCWGC | 1 cut(s) | 662 |
| TspDTI | ATGAA | 3 cut(s) | 540, 585, 788 |
| TspGWI | ACGGA | 2 cut(s) | 127, 243 |
| TspRI | CASTG | 2 cut(s) | 248, 373 |
| Tth111I | GACNNNGTC | 1 cut(s) | 49 |
| XapI | RAATTY | 2 cut(s) | 513, 879 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 340 |
| XmiI | GTMKAC | 1 cut(s) | 353 |
| XspI | CTAG | 3 cut(s) | 329, 426, 582 |
| ZraI | GACGTC | 1 cut(s) | 48 |