Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 478 |
| AccI | GTMKAC | 2 cut(s) | 204, 968 |
| AccII | CGCG | 1 cut(s) | 957 |
| AciI | CCGC | 1 cut(s) | 221 |
| AclWI | GGATC | 2 cut(s) | 449, 1044 |
| AcsI | RAATTY | 2 cut(s) | 1042, 1076 |
| AcuI | CTGAAG | 1 cut(s) | 245 |
| AcvI | CACGTG | 1 cut(s) | 645 |
| AcyI | GRCGYC | 1 cut(s) | 407 |
| AdeI | CACNNNGTG | 1 cut(s) | 478 |
| AfaI | GTAC | 5 cut(s) | 243, 431, 463, 748, 988 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 253, 426, 478, 664, 797 |
| AflIII | ACRYGT | 1 cut(s) | 438 |
| AgsI | TTSAA | 1 cut(s) | 167 |
| AjnI | CCWGG | 4 cut(s) | 69, 669, 679, 1032 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 794, 826 |
| AleI | CACNNNNGTG | 1 cut(s) | 540 |
| AluBI | AGCT | 3 cut(s) | 250, 638, 1111 |
| AluI | AGCT | 3 cut(s) | 250, 638, 1111 |
| Alw26I | GTCTC | 2 cut(s) | 333, 1096 |
| AlwI | GGATC | 2 cut(s) | 449, 1044 |
| AoxI | GGCC | 1 cut(s) | 228 |
| ApeKI | GCWGC | 4 cut(s) | 171, 316, 635, 1150 |
| ApoI | RAATTY | 2 cut(s) | 1042, 1076 |
| AseI | ATTAAT | 1 cut(s) | 500 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 567, 594 |
| AspLEI | GCGC | 2 cut(s) | 957, 1155 |
| AspS9I | GGNCC | 1 cut(s) | 667 |
| AsuC2I | CCSGG | 1 cut(s) | 1167 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 572, 704 |
| AvaII | GGWCC | 1 cut(s) | 667 |
| BbrPI | CACGTG | 1 cut(s) | 645 |
| BbsI | GAAGAC | 1 cut(s) | 748 |
| BbvCI | CCTCAGC | 1 cut(s) | 21 |
| BbvI | GCAGC | 4 cut(s) | 158, 303, 647, 1162 |
| BccI | CCATC | 1 cut(s) | 430 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 484, 518 |
| BciT130I | CCWGG | 4 cut(s) | 71, 671, 681, 1034 |
| BcnI | CCSGG | 1 cut(s) | 1167 |
| BcoDI | GTCTC | 2 cut(s) | 333, 1096 |
| BfmI | CTRYAG | 1 cut(s) | 317 |
| BglII | AGATCT | 1 cut(s) | 135 |
| BisI | GCNGC | 4 cut(s) | 172, 317, 636, 1151 |
| BlsI | GCNGC | 4 cut(s) | 173, 318, 637, 1152 |
| Bme1390I | CCNGG | 5 cut(s) | 71, 671, 681, 1034, 1167 |
| Bme18I | GGWCC | 1 cut(s) | 667 |
| BmgT120I | GGNCC | 1 cut(s) | 667 |
| BmiI | GGNNCC | 4 cut(s) | 291, 852, 975, 1050 |
| BmrFI | CCNGG | 5 cut(s) | 71, 671, 681, 1034, 1167 |
| BmsI | GCATC | 1 cut(s) | 853 |
| BpiI | GAAGAC | 1 cut(s) | 748 |
| Bpu10I | CCTNAGC | 1 cut(s) | 21 |
| BpuEI | CTTGAG | 1 cut(s) | 236 |
| BpuMI | CCSGG | 1 cut(s) | 1167 |
| Bsa29I | ATCGAT | 2 cut(s) | 336, 504 |
| BsaAI | YACGTR | 1 cut(s) | 645 |
| BsaHI | GRCGYC | 1 cut(s) | 407 |
| BsaJI | CCNNGG | 3 cut(s) | 69, 767, 1032 |
| BsaXI | ACNNNNNCTCC | 5 cut(s) | 28, 274, 304, 730, 760 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 253, 426, 478, 664, 797 |
| Bse1I | ACTGG | 2 cut(s) | 284, 325 |
| Bse3DI | GCAATG | 1 cut(s) | 877 |
| BseBI | CCWGG | 4 cut(s) | 71, 671, 681, 1034 |
| BseCI | ATCGAT | 2 cut(s) | 336, 504 |
| BseDI | CCNNGG | 3 cut(s) | 69, 767, 1032 |
| BseGI | GGATG | 1 cut(s) | 840 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 253, 426, 478, 664, 797 |
| BseMI | GCAATG | 1 cut(s) | 877 |
| BseMII | CTCAG | 5 cut(s) | 35, 153, 228, 708, 821 |
| BseNI | ACTGG | 2 cut(s) | 284, 325 |
| BseRI | GAGGAG | 6 cut(s) | 6, 9, 158, 258, 282, 1082 |
| BseXI | GCAGC | 4 cut(s) | 158, 303, 647, 1162 |
| Bsh1236I | CGCG | 1 cut(s) | 957 |
| BshFI | GGCC | 1 cut(s) | 230 |
| BshVI | ATCGAT | 2 cut(s) | 336, 504 |
| BsiSI | CCGG | 1 cut(s) | 1166 |
| BslFI | GGGAC | 1 cut(s) | 1015 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 253, 426, 478, 664, 797 |
| BsmAI | GTCTC | 2 cut(s) | 333, 1096 |
| BsmFI | GGGAC | 1 cut(s) | 1015 |
| BsnI | GGCC | 1 cut(s) | 230 |
| Bsp143I | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| BspACI | CCGC | 1 cut(s) | 221 |
| BspANI | GGCC | 1 cut(s) | 230 |
| BspCNI | CTCAG | 5 cut(s) | 34, 152, 227, 709, 820 |
| BspDI | ATCGAT | 2 cut(s) | 336, 504 |
| BspFNI | CGCG | 1 cut(s) | 957 |
| BspHI | TCATGA | 1 cut(s) | 88 |
| BspLI | GGNNCC | 4 cut(s) | 291, 852, 975, 1050 |
| BspMAI | CTGCAG | 1 cut(s) | 321 |
| BspPI | GGATC | 2 cut(s) | 449, 1044 |
| BsrDI | GCAATG | 1 cut(s) | 877 |
| BsrI | ACTGG | 2 cut(s) | 284, 325 |
| BssECI | CCNNGG | 3 cut(s) | 69, 767, 1032 |
| BssMI | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| BssNI | GRCGYC | 1 cut(s) | 407 |
| Bst2UI | CCWGG | 4 cut(s) | 71, 671, 681, 1034 |
| Bst4CI | ACNGT | 6 cut(s) | 119, 286, 478, 751, 995, 1084 |
| BstACI | GRCGYC | 1 cut(s) | 407 |
| BstBAI | YACGTR | 1 cut(s) | 645 |
| BstC8I | GCNNGC | 2 cut(s) | 493, 868 |
| BstDEI | CTNAG | 8 cut(s) | 21, 139, 214, 389, 717, 807, 1011, 1134 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 795 |
| BstF5I | GGATG | 1 cut(s) | 840 |
| BstFNI | CGCG | 1 cut(s) | 957 |
| BstHHI | GCGC | 2 cut(s) | 957, 1155 |
| BstKTI | GATC | 4 cut(s) | 138, 457, 604, 1039 |
| BstMAI | GTCTC | 2 cut(s) | 333, 1096 |
| BstMBI | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 391, 685, 778, 872 |
| BstNI | CCWGG | 4 cut(s) | 71, 671, 681, 1034 |
| BstNSI | RCATGY | 2 cut(s) | 635, 734 |
| BstSCI | CCNGG | 5 cut(s) | 69, 669, 679, 1032, 1165 |
| BstSFI | CTRYAG | 1 cut(s) | 317 |
| BstUI | CGCG | 1 cut(s) | 957 |
| BstV1I | GCAGC | 4 cut(s) | 158, 303, 647, 1162 |
| BstV2I | GAAGAC | 1 cut(s) | 748 |
| BstX2I | RGATCY | 2 cut(s) | 135, 1036 |
| BstYI | RGATCY | 2 cut(s) | 135, 1036 |
| Bsu15I | ATCGAT | 2 cut(s) | 336, 504 |
| BsuRI | GGCC | 1 cut(s) | 230 |
| BsuTUI | ATCGAT | 2 cut(s) | 336, 504 |
| BtsCI | GGATG | 1 cut(s) | 840 |
| BtsIMutI | CAGTG | 3 cut(s) | 291, 365, 474 |
| Cac8I | GCNNGC | 2 cut(s) | 493, 868 |
| CciI | TCATGA | 1 cut(s) | 88 |
| CfoI | GCGC | 2 cut(s) | 957, 1155 |
| Cfr13I | GGNCC | 1 cut(s) | 667 |
| ClaI | ATCGAT | 2 cut(s) | 336, 504 |
| CseI | GACGC | 1 cut(s) | 415 |
| Csp6I | GTAC | 5 cut(s) | 242, 430, 462, 747, 987 |
| CviAII | CATG | 7 cut(s) | 89, 194, 313, 632, 689, 731, 941 |
| CviQI | GTAC | 5 cut(s) | 242, 430, 462, 747, 987 |
| DdeI | CTNAG | 8 cut(s) | 21, 139, 214, 389, 717, 807, 1011, 1134 |
| DpnI | GATC | 4 cut(s) | 137, 456, 603, 1038 |
| DpnII | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| DraIII | CACNNNGTG | 1 cut(s) | 478 |
| Eco47I | GGWCC | 1 cut(s) | 667 |
| Eco57I | CTGAAG | 1 cut(s) | 245 |
| Eco72I | CACGTG | 1 cut(s) | 645 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 795 |
| EcoRII | CCWGG | 4 cut(s) | 69, 669, 679, 1032 |
| FaeI | CATG | 7 cut(s) | 92, 197, 316, 635, 692, 734, 944 |
| FaqI | GGGAC | 1 cut(s) | 1015 |
| FatI | CATG | 7 cut(s) | 88, 193, 312, 631, 688, 730, 940 |
| FblI | GTMKAC | 2 cut(s) | 204, 968 |
| Fnu4HI | GCNGC | 4 cut(s) | 172, 317, 636, 1151 |
| FokI | GGATG | 1 cut(s) | 827 |
| Fsp4HI | GCNGC | 4 cut(s) | 172, 317, 636, 1151 |
| GlaI | GCGC | 2 cut(s) | 956, 1154 |
| GluI | GCNGC | 4 cut(s) | 172, 317, 636, 1151 |
| HaeIII | GGCC | 1 cut(s) | 230 |
| HapII | CCGG | 1 cut(s) | 1166 |
| HgaI | GACGC | 1 cut(s) | 415 |
| HhaI | GCGC | 2 cut(s) | 957, 1155 |
| Hin1I | GRCGYC | 1 cut(s) | 407 |
| Hin1II | CATG | 7 cut(s) | 92, 197, 316, 635, 692, 734, 944 |
| Hin6I | GCGC | 2 cut(s) | 955, 1153 |
| HinP1I | GCGC | 2 cut(s) | 955, 1153 |
| HincII | GTYRAC | 1 cut(s) | 511 |
| HindII | GTYRAC | 1 cut(s) | 511 |
| HinfI | GANTC | 6 cut(s) | 7, 65, 146, 259, 590, 965 |
| HpaII | CCGG | 1 cut(s) | 1166 |
| HphI | GGTGA | 3 cut(s) | 16, 572, 704 |
| Hpy166II | GTNNAC | 8 cut(s) | 161, 205, 244, 365, 511, 547, 969, 1130 |
| Hpy188I | TCNGA | 4 cut(s) | 135, 142, 217, 928 |
| Hpy188III | TCNNGA | 4 cut(s) | 89, 599, 791, 949 |
| Hpy8I | GTNNAC | 8 cut(s) | 161, 205, 244, 365, 511, 547, 969, 1130 |
| Hpy99I | CGWCG | 1 cut(s) | 765 |
| HpyAV | CCTTC | 6 cut(s) | 164, 220, 295, 809, 987, 1051 |
| HpyCH4III | ACNGT | 6 cut(s) | 119, 286, 478, 751, 995, 1084 |
| HpyCH4IV | ACGT | 4 cut(s) | 440, 644, 745, 795 |
| HpyCH4V | TGCA | 7 cut(s) | 83, 174, 319, 343, 635, 648, 688 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 391, 685, 778, 872 |
| HpyF3I | CTNAG | 8 cut(s) | 21, 139, 214, 389, 717, 807, 1011, 1134 |
| HpySE526I | ACGT | 4 cut(s) | 440, 644, 745, 795 |
| Hsp92I | GRCGYC | 1 cut(s) | 407 |
| Hsp92II | CATG | 7 cut(s) | 92, 197, 316, 635, 692, 734, 944 |
| HspAI | GCGC | 2 cut(s) | 955, 1153 |
| Kzo9I | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| LmnI | GCTCC | 2 cut(s) | 295, 1048 |
| Lsp1109I | GCAGC | 4 cut(s) | 158, 303, 647, 1162 |
| LweI | GCATC | 1 cut(s) | 853 |
| MaeII | ACGT | 4 cut(s) | 440, 644, 745, 795 |
| MaeIII | GTNAC | 1 cut(s) | 4 |
| MalI | GATC | 4 cut(s) | 137, 456, 603, 1038 |
| MboI | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| MboII | GAAGA | 4 cut(s) | 254, 753, 912, 972 |
| MflI | RGATCY | 2 cut(s) | 135, 1036 |
| MluCI | AATT | 6 cut(s) | 84, 371, 450, 881, 1042, 1076 |
| MlyI | GAGTC | 2 cut(s) | 155, 974 |
| MmeI | TCCRAC | 3 cut(s) | 535, 792, 858 |
| MroXI | GAANNNNTTC | 2 cut(s) | 567, 594 |
| MseI | TTAA | 3 cut(s) | 75, 273, 500 |
| MslI | CAYNNNNRTG | 3 cut(s) | 540, 542, 845 |
| MspA1I | CMGCKG | 1 cut(s) | 223 |
| MspI | CCGG | 1 cut(s) | 1166 |
| MspR9I | CCNGG | 5 cut(s) | 71, 671, 681, 1034, 1167 |
| MvaI | CCWGG | 4 cut(s) | 71, 671, 681, 1034 |
| MvnI | CGCG | 1 cut(s) | 957 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 391, 685, 778, 872 |
| NciI | CCSGG | 1 cut(s) | 1167 |
| NdeII | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| NlaIII | CATG | 7 cut(s) | 92, 197, 316, 635, 692, 734, 944 |
| NlaIV | GGNNCC | 4 cut(s) | 291, 852, 975, 1050 |
| NmeAIII | GCCGAG | 1 cut(s) | 748 |
| NmuCI | GTSAC | 1 cut(s) | 4 |
| NspI | RCATGY | 2 cut(s) | 635, 734 |
| OliI | CACNNNNGTG | 1 cut(s) | 540 |
| PagI | TCATGA | 1 cut(s) | 88 |
| PdmI | GAANNNNTTC | 2 cut(s) | 567, 594 |
| PfeI | GAWTC | 3 cut(s) | 65, 259, 590 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 478 |
| PkrI | GCNGC | 4 cut(s) | 173, 318, 637, 1152 |
| PleI | GAGTC | 2 cut(s) | 154, 973 |
| PmaCI | CACGTG | 1 cut(s) | 645 |
| PmlI | CACGTG | 1 cut(s) | 645 |
| PpsI | GAGTC | 2 cut(s) | 154, 973 |
| Ppu21I | YACGTR | 1 cut(s) | 645 |
| PshBI | ATTAAT | 1 cut(s) | 500 |
| Psp6I | CCWGG | 4 cut(s) | 69, 669, 679, 1032 |
| PspCI | CACGTG | 1 cut(s) | 645 |
| PspGI | CCWGG | 4 cut(s) | 69, 669, 679, 1032 |
| PspN4I | GGNNCC | 4 cut(s) | 291, 852, 975, 1050 |
| PspPI | GGNCC | 1 cut(s) | 667 |
| PstI | CTGCAG | 1 cut(s) | 321 |
| PsuI | RGATCY | 2 cut(s) | 135, 1036 |
| RsaI | GTAC | 5 cut(s) | 243, 431, 463, 748, 988 |
| RsaNI | GTAC | 5 cut(s) | 242, 430, 462, 747, 987 |
| RseI | CAYNNNNRTG | 3 cut(s) | 540, 542, 845 |
| SaqAI | TTAA | 3 cut(s) | 75, 273, 500 |
| SatI | GCNGC | 4 cut(s) | 172, 317, 636, 1151 |
| Sau3AI | GATC | 4 cut(s) | 135, 454, 601, 1036 |
| Sau96I | GGNCC | 1 cut(s) | 667 |
| SchI | GAGTC | 2 cut(s) | 155, 974 |
| ScrFI | CCNGG | 5 cut(s) | 71, 671, 681, 1034, 1167 |
| SfaNI | GCATC | 1 cut(s) | 853 |
| SfcI | CTRYAG | 1 cut(s) | 317 |
| SinI | GGWCC | 1 cut(s) | 667 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 540, 542, 845 |
| SmlI | CTYRAG | 1 cut(s) | 251 |
| SmoI | CTYRAG | 1 cut(s) | 251 |
| Sse9I | AATT | 6 cut(s) | 84, 371, 450, 881, 1042, 1076 |
| SsiI | CCGC | 1 cut(s) | 221 |
| StyD4I | CCNGG | 5 cut(s) | 69, 669, 679, 1032, 1165 |
| TaaI | ACNGT | 6 cut(s) | 119, 286, 478, 751, 995, 1084 |
| TaiI | ACGT | 4 cut(s) | 443, 647, 748, 798 |
| TaqI | TCGA | 3 cut(s) | 336, 504, 983 |
| TasI | AATT | 6 cut(s) | 84, 371, 450, 881, 1042, 1076 |
| TfiI | GAWTC | 3 cut(s) | 65, 259, 590 |
| Tru1I | TTAA | 3 cut(s) | 75, 273, 500 |
| Tru9I | TTAA | 3 cut(s) | 75, 273, 500 |
| TscAI | CASTG | 3 cut(s) | 291, 372, 481 |
| TseFI | GTSAC | 1 cut(s) | 4 |
| TseI | GCWGC | 4 cut(s) | 171, 316, 635, 1150 |
| Tsp45I | GTSAC | 1 cut(s) | 4 |
| TspDTI | ATGAA | 4 cut(s) | 77, 301, 603, 990 |
| TspGWI | ACGGA | 1 cut(s) | 986 |
| TspRI | CASTG | 3 cut(s) | 291, 372, 481 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 478 |
| VpaK11BI | GGWCC | 1 cut(s) | 667 |
| VspI | ATTAAT | 1 cut(s) | 500 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 795 |
| XapI | RAATTY | 2 cut(s) | 1042, 1076 |
| XceI | RCATGY | 2 cut(s) | 635, 734 |
| XmiI | GTMKAC | 2 cut(s) | 204, 968 |
| XmnI | GAANNNNTTC | 2 cut(s) | 567, 594 |