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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 1 cut(s) | 13 |
| AgsI | TTSAA | 3 cut(s) | 151, 173, 183 |
| AseI | ATTAAT | 1 cut(s) | 12 |
| AspS9I | GGNCC | 1 cut(s) | 89 |
| AvaII | GGWCC | 1 cut(s) | 89 |
| BauI | CACGAG | 1 cut(s) | 102 |
| BccI | CCATC | 1 cut(s) | 211 |
| BfmI | CTRYAG | 1 cut(s) | 123 |
| BfuAI | ACCTGC | 1 cut(s) | 13 |
| Bme18I | GGWCC | 1 cut(s) | 89 |
| BmgT120I | GGNCC | 1 cut(s) | 89 |
| BmiI | GGNNCC | 1 cut(s) | 90 |
| BmsI | GCATC | 2 cut(s) | 67, 116 |
| BseGI | GGATG | 1 cut(s) | 222 |
| BseMII | CTCAG | 2 cut(s) | 9, 236 |
| BseRI | GAGGAG | 2 cut(s) | 102, 105 |
| BsmI | GAATGC | 1 cut(s) | 76 |
| BspCNI | CTCAG | 2 cut(s) | 10, 235 |
| BspLI | GGNNCC | 1 cut(s) | 90 |
| BspMI | ACCTGC | 1 cut(s) | 13 |
| BssSI | CACGAG | 1 cut(s) | 102 |
| Bst2BI | CACGAG | 1 cut(s) | 102 |
| Bst4CI | ACNGT | 1 cut(s) | 124 |
| BstDEI | CTNAG | 3 cut(s) | 18, 199, 222 |
| BstF5I | GGATG | 1 cut(s) | 222 |
| BstSFI | CTRYAG | 1 cut(s) | 123 |
| BtsCI | GGATG | 1 cut(s) | 222 |
| BtsI | GCAGTG | 1 cut(s) | 135 |
| BtsIMutI | CAGTG | 1 cut(s) | 135 |
| BveI | ACCTGC | 1 cut(s) | 13 |
| Cfr13I | GGNCC | 1 cut(s) | 89 |
| CviJI | RGCY | 1 cut(s) | 221 |
| CviKI_1 | RGCY | 1 cut(s) | 221 |
| DdeI | CTNAG | 3 cut(s) | 18, 199, 222 |
| Eco47I | GGWCC | 1 cut(s) | 89 |
| EcoO109I | RGGNCCY | 1 cut(s) | 89 |
| FaiI | YATR | 2 cut(s) | 59, 161 |
| FokI | GGATG | 1 cut(s) | 229 |
| HinfI | GANTC | 1 cut(s) | 140 |
| Hpy166II | GTNNAC | 1 cut(s) | 207 |
| Hpy188I | TCNGA | 2 cut(s) | 145, 225 |
| Hpy8I | GTNNAC | 1 cut(s) | 207 |
| HpyAV | CCTTC | 1 cut(s) | 102 |
| HpyCH4III | ACNGT | 1 cut(s) | 124 |
| HpyCH4IV | ACGT | 1 cut(s) | 209 |
| HpyCH4V | TGCA | 2 cut(s) | 74, 188 |
| HpyF3I | CTNAG | 3 cut(s) | 18, 199, 222 |
| HpySE526I | ACGT | 1 cut(s) | 209 |
| LpnPI | CCDG | 2 cut(s) | 8, 94 |
| LweI | GCATC | 2 cut(s) | 67, 116 |
| MaeII | ACGT | 1 cut(s) | 209 |
| MboII | GAAGA | 1 cut(s) | 52 |
| MfeI | CAATTG | 1 cut(s) | 183 |
| MluCI | AATT | 2 cut(s) | 146, 183 |
| MnlI | CCTC | 3 cut(s) | 79, 123, 126 |
| MseI | TTAA | 2 cut(s) | 12, 244 |
| MunI | CAATTG | 1 cut(s) | 183 |
| Mva1269I | GAATGC | 1 cut(s) | 76 |
| NlaIV | GGNNCC | 1 cut(s) | 90 |
| PaqCI | CACCTGC | 1 cut(s) | 13 |
| PctI | GAATGC | 1 cut(s) | 76 |
| PfeI | GAWTC | 1 cut(s) | 140 |
| PpuMI | RGGWCCY | 1 cut(s) | 89 |
| PshBI | ATTAAT | 1 cut(s) | 12 |
| Psp5II | RGGWCCY | 1 cut(s) | 89 |
| PspN4I | GGNNCC | 1 cut(s) | 90 |
| PspPI | GGNCC | 1 cut(s) | 89 |
| PspPPI | RGGWCCY | 1 cut(s) | 89 |
| SaqAI | TTAA | 2 cut(s) | 12, 244 |
| Sau96I | GGNCC | 1 cut(s) | 89 |
| SetI | ASST | 2 cut(s) | 27, 212 |
| SfaNI | GCATC | 2 cut(s) | 67, 116 |
| SfcI | CTRYAG | 1 cut(s) | 123 |
| SgeI | CNNG | 6 cut(s) | 35, 93, 114, 116, 166, 201 |
| SinI | GGWCC | 1 cut(s) | 89 |
| Sse9I | AATT | 2 cut(s) | 146, 183 |
| TaaI | ACNGT | 1 cut(s) | 124 |
| TaiI | ACGT | 1 cut(s) | 212 |
| TasI | AATT | 2 cut(s) | 146, 183 |
| TfiI | GAWTC | 1 cut(s) | 140 |
| Tru1I | TTAA | 2 cut(s) | 12, 244 |
| Tru9I | TTAA | 2 cut(s) | 12, 244 |
| TscAI | CASTG | 1 cut(s) | 135 |
| TspDTI | ATGAA | 1 cut(s) | 84 |
| TspRI | CASTG | 1 cut(s) | 135 |
| VpaK11BI | GGWCC | 1 cut(s) | 89 |
| VspI | ATTAAT | 1 cut(s) | 12 |