Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 369 |
| AccI | GTMKAC | 1 cut(s) | 60 |
| AclWI | GGATC | 3 cut(s) | 38, 306, 358 |
| AcoI | YGGCCR | 1 cut(s) | 499 |
| AcvI | CACGTG | 2 cut(s) | 708, 735 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 70, 369, 708, 763, 976, 979 |
| AflIII | ACRYGT | 1 cut(s) | 705 |
| AgsI | TTSAA | 6 cut(s) | 223, 401, 414, 743, 762, 929 |
| AjnI | CCWGG | 3 cut(s) | 50, 69, 747 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 616, 648 |
| AluBI | AGCT | 9 cut(s) | 13, 24, 130, 218, 596, 793, 829, 836, 1043 |
| AluI | AGCT | 9 cut(s) | 13, 24, 130, 218, 596, 793, 829, 836, 1043 |
| Alw21I | GWGCWC | 2 cut(s) | 598, 662 |
| Alw26I | GTCTC | 3 cut(s) | 21, 533, 680 |
| Alw44I | GTGCAC | 1 cut(s) | 658 |
| AlwI | GGATC | 3 cut(s) | 38, 306, 358 |
| Ama87I | CYCGRG | 1 cut(s) | 945 |
| AoxI | GGCC | 5 cut(s) | 245, 499, 554, 694, 781 |
| ApaLI | GTGCAC | 1 cut(s) | 658 |
| ApeKI | GCWGC | 2 cut(s) | 421, 821 |
| AspLEI | GCGC | 1 cut(s) | 1009 |
| AspS9I | GGNCC | 4 cut(s) | 358, 554, 781, 950 |
| AsuHPI | GGTGA | 1 cut(s) | 104 |
| AvaI | CYCGRG | 1 cut(s) | 945 |
| AvaII | GGWCC | 2 cut(s) | 358, 950 |
| BaeGI | GKGCMC | 1 cut(s) | 662 |
| BanII | GRGCYC | 1 cut(s) | 598 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 220, 252 |
| BbrPI | CACGTG | 2 cut(s) | 708, 735 |
| Bbv12I | GWGCWC | 2 cut(s) | 598, 662 |
| BbvI | GCAGC | 2 cut(s) | 433, 833 |
| BccI | CCATC | 3 cut(s) | 35, 521, 914 |
| BceAI | ACGGC | 1 cut(s) | 486 |
| BciT130I | CCWGG | 3 cut(s) | 52, 71, 749 |
| BciVI | GTATCC | 2 cut(s) | 199, 895 |
| BcoDI | GTCTC | 3 cut(s) | 21, 533, 680 |
| BfaI | CTAG | 2 cut(s) | 906, 1044 |
| BfmI | CTRYAG | 1 cut(s) | 471 |
| BfuI | GTATCC | 2 cut(s) | 199, 895 |
| BglII | AGATCT | 1 cut(s) | 494 |
| BisI | GCNGC | 2 cut(s) | 422, 822 |
| BlsI | GCNGC | 2 cut(s) | 423, 823 |
| Bme1390I | CCNGG | 3 cut(s) | 52, 71, 749 |
| Bme18I | GGWCC | 2 cut(s) | 358, 950 |
| BmeT110I | CYCGRG | 1 cut(s) | 945 |
| BmgT120I | GGNCC | 4 cut(s) | 358, 554, 781, 950 |
| BmiI | GGNNCC | 5 cut(s) | 234, 270, 360, 782, 951 |
| BmrFI | CCNGG | 3 cut(s) | 52, 71, 749 |
| BmsI | GCATC | 1 cut(s) | 156 |
| BpmI | CTGGAG | 1 cut(s) | 328 |
| BsaAI | YACGTR | 2 cut(s) | 708, 735 |
| BsaBI | GATNNNNATC | 1 cut(s) | 885 |
| BsaJI | CCNNGG | 3 cut(s) | 187, 315, 557 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 29 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 70, 369, 708, 763, 976, 979 |
| Bse1I | ACTGG | 1 cut(s) | 863 |
| Bse8I | GATNNNNATC | 1 cut(s) | 885 |
| BseBI | CCWGG | 3 cut(s) | 52, 71, 749 |
| BseDI | CCNNGG | 3 cut(s) | 187, 315, 557 |
| BseGI | GGATG | 4 cut(s) | 79, 532, 886, 925 |
| BseJI | GATNNNNATC | 1 cut(s) | 885 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 70, 369, 708, 763, 976, 979 |
| BseMII | CTCAG | 4 cut(s) | 368, 633, 810, 971 |
| BseNI | ACTGG | 1 cut(s) | 863 |
| BseRI | GAGGAG | 2 cut(s) | 23, 992 |
| BseSI | GKGCMC | 1 cut(s) | 662 |
| BseXI | GCAGC | 2 cut(s) | 433, 833 |
| BshFI | GGCC | 5 cut(s) | 247, 501, 556, 696, 783 |
| BsiHKAI | GWGCWC | 2 cut(s) | 598, 662 |
| BsiHKCI | CYCGRG | 1 cut(s) | 945 |
| BslFI | GGGAC | 1 cut(s) | 311 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 70, 369, 708, 763, 976, 979 |
| BsmAI | GTCTC | 3 cut(s) | 21, 533, 680 |
| BsmFI | GGGAC | 1 cut(s) | 311 |
| BsnI | GGCC | 5 cut(s) | 247, 501, 556, 696, 783 |
| BsoBI | CYCGRG | 1 cut(s) | 945 |
| Bsp1286I | GDGCHC | 2 cut(s) | 598, 662 |
| Bsp143I | GATC | 4 cut(s) | 43, 311, 350, 494 |
| Bsp19I | CCATGG | 1 cut(s) | 187 |
| BspANI | GGCC | 5 cut(s) | 247, 501, 556, 696, 783 |
| BspCNI | CTCAG | 4 cut(s) | 367, 634, 809, 970 |
| BspHI | TCATGA | 1 cut(s) | 598 |
| BspLI | GGNNCC | 5 cut(s) | 234, 270, 360, 782, 951 |
| BspPI | GGATC | 3 cut(s) | 38, 306, 358 |
| BsrI | ACTGG | 1 cut(s) | 863 |
| BssECI | CCNNGG | 3 cut(s) | 187, 315, 557 |
| BssMI | GATC | 4 cut(s) | 43, 311, 350, 494 |
| BssT1I | CCWWGG | 3 cut(s) | 187, 315, 557 |
| Bst2UI | CCWGG | 3 cut(s) | 52, 71, 749 |
| Bst4CI | ACNGT | 2 cut(s) | 227, 475 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 666 |
| BstBAI | YACGTR | 2 cut(s) | 708, 735 |
| BstC8I | GCNNGC | 1 cut(s) | 216 |
| BstDEI | CTNAG | 4 cut(s) | 354, 642, 796, 957 |
| BstDSI | CCRYGG | 1 cut(s) | 187 |
| BstF5I | GGATG | 4 cut(s) | 79, 532, 886, 925 |
| BstHHI | GCGC | 1 cut(s) | 1009 |
| BstKTI | GATC | 4 cut(s) | 46, 314, 353, 497 |
| BstMAI | GTCTC | 3 cut(s) | 21, 533, 680 |
| BstMBI | GATC | 4 cut(s) | 43, 311, 350, 494 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 136, 666, 1015 |
| BstNI | CCWGG | 3 cut(s) | 52, 71, 749 |
| BstSCI | CCNGG | 3 cut(s) | 50, 69, 747 |
| BstSFI | CTRYAG | 1 cut(s) | 471 |
| BstSLI | GKGCMC | 1 cut(s) | 662 |
| BstV1I | GCAGC | 2 cut(s) | 433, 833 |
| BstX2I | RGATCY | 3 cut(s) | 311, 350, 494 |
| BstYI | RGATCY | 3 cut(s) | 311, 350, 494 |
| BsuI | GTATCC | 2 cut(s) | 199, 895 |
| BsuRI | GGCC | 5 cut(s) | 247, 501, 556, 696, 783 |
| BtgI | CCRYGG | 1 cut(s) | 187 |
| BtsCI | GGATG | 4 cut(s) | 79, 532, 886, 925 |
| Cac8I | GCNNGC | 1 cut(s) | 216 |
| CciI | TCATGA | 1 cut(s) | 598 |
| CfoI | GCGC | 1 cut(s) | 1009 |
| Cfr13I | GGNCC | 4 cut(s) | 358, 554, 781, 950 |
| CviAII | CATG | 6 cut(s) | 188, 383, 551, 599, 932, 1015 |
| DdeI | CTNAG | 4 cut(s) | 354, 642, 796, 957 |
| DpnI | GATC | 4 cut(s) | 45, 313, 352, 496 |
| DpnII | GATC | 4 cut(s) | 43, 311, 350, 494 |
| DraI | TTTAAA | 2 cut(s) | 840, 852 |
| EaeI | YGGCCR | 1 cut(s) | 499 |
| Ecl136II | GAGCTC | 1 cut(s) | 596 |
| Eco130I | CCWWGG | 3 cut(s) | 187, 315, 557 |
| Eco147I | AGGCCT | 2 cut(s) | 247, 696 |
| Eco24I | GRGCYC | 1 cut(s) | 598 |
| Eco47I | GGWCC | 2 cut(s) | 358, 950 |
| Eco53kI | GAGCTC | 1 cut(s) | 596 |
| Eco72I | CACGTG | 2 cut(s) | 708, 735 |
| Eco88I | CYCGRG | 1 cut(s) | 945 |
| EcoICRI | GAGCTC | 1 cut(s) | 596 |
| EcoO109I | RGGNCCY | 2 cut(s) | 358, 950 |
| EcoRII | CCWGG | 3 cut(s) | 50, 69, 747 |
| EcoT14I | CCWWGG | 3 cut(s) | 187, 315, 557 |
| EcoT38I | GRGCYC | 1 cut(s) | 598 |
| ErhI | CCWWGG | 3 cut(s) | 187, 315, 557 |
| FaeI | CATG | 6 cut(s) | 191, 386, 554, 602, 935, 1018 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 616, 648 |
| FaqI | GGGAC | 1 cut(s) | 311 |
| FatI | CATG | 6 cut(s) | 187, 382, 550, 598, 931, 1014 |
| FauNDI | CATATG | 1 cut(s) | 40 |
| FblI | GTMKAC | 1 cut(s) | 60 |
| Fnu4HI | GCNGC | 2 cut(s) | 422, 822 |
| FokI | GGATG | 4 cut(s) | 86, 539, 893, 932 |
| FriOI | GRGCYC | 1 cut(s) | 598 |
| Fsp4HI | GCNGC | 2 cut(s) | 422, 822 |
| FspBI | CTAG | 2 cut(s) | 906, 1044 |
| GlaI | GCGC | 1 cut(s) | 1008 |
| GluI | GCNGC | 2 cut(s) | 422, 822 |
| GsuI | CTGGAG | 1 cut(s) | 328 |
| HaeIII | GGCC | 5 cut(s) | 247, 501, 556, 696, 783 |
| HhaI | GCGC | 1 cut(s) | 1009 |
| Hin1II | CATG | 6 cut(s) | 191, 386, 554, 602, 935, 1018 |
| Hin6I | GCGC | 1 cut(s) | 1007 |
| HinP1I | GCGC | 1 cut(s) | 1007 |
| HincII | GTYRAC | 1 cut(s) | 61 |
| HindII | GTYRAC | 1 cut(s) | 61 |
| HindIII | AAGCTT | 2 cut(s) | 128, 827 |
| HinfI | GANTC | 3 cut(s) | 100, 453, 1047 |
| HphI | GGTGA | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 4 cut(s) | 61, 660, 674, 711 |
| Hpy188I | TCNGA | 3 cut(s) | 395, 643, 1052 |
| Hpy188III | TCNNGA | 5 cut(s) | 307, 356, 492, 599, 959 |
| Hpy8I | GTNNAC | 4 cut(s) | 61, 660, 674, 711 |
| HpyAV | CCTTC | 3 cut(s) | 83, 246, 963 |
| HpyCH4III | ACNGT | 2 cut(s) | 227, 475 |
| HpyCH4IV | ACGT | 2 cut(s) | 707, 734 |
| HpyCH4V | TGCA | 6 cut(s) | 169, 620, 660, 669, 821, 1018 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 136, 666, 1015 |
| HpyF3I | CTNAG | 4 cut(s) | 354, 642, 796, 957 |
| HpySE526I | ACGT | 2 cut(s) | 707, 734 |
| Hsp92II | CATG | 6 cut(s) | 191, 386, 554, 602, 935, 1018 |
| HspAI | GCGC | 1 cut(s) | 1007 |
| Kzo9I | GATC | 4 cut(s) | 43, 311, 350, 494 |
| LmnI | GCTCC | 3 cut(s) | 10, 29, 593 |
| Lsp1109I | GCAGC | 2 cut(s) | 433, 833 |
| LweI | GCATC | 1 cut(s) | 156 |
| MaeI | CTAG | 2 cut(s) | 906, 1044 |
| MaeII | ACGT | 2 cut(s) | 707, 734 |
| MaeIII | GTNAC | 1 cut(s) | 628 |
| MalI | GATC | 4 cut(s) | 45, 313, 352, 496 |
| MboI | GATC | 4 cut(s) | 43, 311, 350, 494 |
| MboII | GAAGA | 4 cut(s) | 98, 279, 402, 992 |
| MfeI | CAATTG | 1 cut(s) | 414 |
| MflI | RGATCY | 3 cut(s) | 311, 350, 494 |
| MhlI | GDGCHC | 2 cut(s) | 598, 662 |
| MluCI | AATT | 7 cut(s) | 95, 414, 580, 664, 962, 988, 994 |
| MlyI | GAGTC | 2 cut(s) | 94, 462 |
| MmeI | TCCRAC | 3 cut(s) | 246, 504, 897 |
| MseI | TTAA | 5 cut(s) | 132, 534, 579, 839, 851 |
| MspR9I | CCNGG | 3 cut(s) | 52, 71, 749 |
| MunI | CAATTG | 1 cut(s) | 414 |
| MvaI | CCWGG | 3 cut(s) | 52, 71, 749 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 136, 666, 1015 |
| NcoI | CCATGG | 1 cut(s) | 187 |
| NdeI | CATATG | 1 cut(s) | 40 |
| NdeII | GATC | 4 cut(s) | 43, 311, 350, 494 |
| NlaIII | CATG | 6 cut(s) | 191, 386, 554, 602, 935, 1018 |
| NlaIV | GGNNCC | 5 cut(s) | 234, 270, 360, 782, 951 |
| PagI | TCATGA | 1 cut(s) | 598 |
| PceI | AGGCCT | 2 cut(s) | 247, 696 |
| PflFI | GACNNNGTC | 1 cut(s) | 65 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 369 |
| PfoI | TCCNGGA | 1 cut(s) | 69 |
| PkrI | GCNGC | 2 cut(s) | 423, 823 |
| PleI | GAGTC | 2 cut(s) | 94, 461 |
| PmaCI | CACGTG | 2 cut(s) | 708, 735 |
| PmlI | CACGTG | 2 cut(s) | 708, 735 |
| PpsI | GAGTC | 2 cut(s) | 94, 461 |
| Ppu21I | YACGTR | 2 cut(s) | 708, 735 |
| PpuMI | RGGWCCY | 2 cut(s) | 358, 950 |
| Psp124BI | GAGCTC | 1 cut(s) | 598 |
| Psp5II | RGGWCCY | 2 cut(s) | 358, 950 |
| Psp6I | CCWGG | 3 cut(s) | 50, 69, 747 |
| PspCI | CACGTG | 2 cut(s) | 708, 735 |
| PspGI | CCWGG | 3 cut(s) | 50, 69, 747 |
| PspN4I | GGNNCC | 5 cut(s) | 234, 270, 360, 782, 951 |
| PspPI | GGNCC | 4 cut(s) | 358, 554, 781, 950 |
| PspPPI | RGGWCCY | 2 cut(s) | 358, 950 |
| PsuI | RGATCY | 3 cut(s) | 311, 350, 494 |
| PsyI | GACNNNGTC | 1 cut(s) | 65 |
| SacI | GAGCTC | 1 cut(s) | 598 |
| SalI | GTCGAC | 1 cut(s) | 59 |
| SaqAI | TTAA | 5 cut(s) | 132, 534, 579, 839, 851 |
| SatI | GCNGC | 2 cut(s) | 422, 822 |
| Sau3AI | GATC | 4 cut(s) | 43, 311, 350, 494 |
| Sau96I | GGNCC | 4 cut(s) | 358, 554, 781, 950 |
| SchI | GAGTC | 2 cut(s) | 94, 462 |
| ScrFI | CCNGG | 3 cut(s) | 52, 71, 749 |
| SduI | GDGCHC | 2 cut(s) | 598, 662 |
| SfaNI | GCATC | 1 cut(s) | 156 |
| SfcI | CTRYAG | 1 cut(s) | 471 |
| SinI | GGWCC | 2 cut(s) | 358, 950 |
| Sse9I | AATT | 7 cut(s) | 95, 414, 580, 664, 962, 988, 994 |
| SseBI | AGGCCT | 2 cut(s) | 247, 696 |
| SspMI | CTAG | 2 cut(s) | 906, 1044 |
| SstI | GAGCTC | 1 cut(s) | 598 |
| StuI | AGGCCT | 2 cut(s) | 247, 696 |
| StyD4I | CCNGG | 3 cut(s) | 50, 69, 747 |
| StyI | CCWWGG | 3 cut(s) | 187, 315, 557 |
| TaaI | ACNGT | 2 cut(s) | 227, 475 |
| TaiI | ACGT | 2 cut(s) | 710, 737 |
| TaqI | TCGA | 3 cut(s) | 60, 447, 832 |
| TasI | AATT | 7 cut(s) | 95, 414, 580, 664, 962, 988, 994 |
| Tru1I | TTAA | 5 cut(s) | 132, 534, 579, 839, 851 |
| Tru9I | TTAA | 5 cut(s) | 132, 534, 579, 839, 851 |
| TseI | GCWGC | 2 cut(s) | 421, 821 |
| TspDTI | ATGAA | 3 cut(s) | 539, 615, 948 |
| TspGWI | ACGGA | 1 cut(s) | 1022 |
| Tth111I | GACNNNGTC | 1 cut(s) | 65 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 369 |
| VneI | GTGCAC | 1 cut(s) | 658 |
| VpaK11BI | GGWCC | 2 cut(s) | 358, 950 |
| XmiI | GTMKAC | 1 cut(s) | 60 |
| XspI | CTAG | 2 cut(s) | 906, 1044 |