Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1157 |
| Acc36I | ACCTGC | 1 cut(s) | 1157 |
| AccB1I | GGYRCC | 1 cut(s) | 359 |
| AccBSI | CCGCTC | 2 cut(s) | 561, 872 |
| AccI | GTMKAC | 1 cut(s) | 925 |
| AccII | CGCG | 2 cut(s) | 197, 1311 |
| AciI | CCGC | 8 cut(s) | 151, 388, 457, 559, 870, 950, 977, 1067 |
| AclWI | GGATC | 3 cut(s) | 836, 849, 949 |
| AcoI | YGGCCR | 1 cut(s) | 987 |
| AcsI | RAATTY | 1 cut(s) | 301 |
| AcuI | CTGAAG | 1 cut(s) | 729 |
| AcyI | GRCGYC | 1 cut(s) | 808 |
| AfaI | GTAC | 1 cut(s) | 1003 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 463, 565, 1304 |
| AflII | CTTAAG | 1 cut(s) | 248 |
| AgsI | TTSAA | 7 cut(s) | 110, 323, 671, 758, 1049, 1175, 1321 |
| AjiI | CACGTC | 1 cut(s) | 773 |
| AjnI | CCWGG | 3 cut(s) | 578, 844, 1039 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 639, 671 |
| Alw26I | GTCTC | 6 cut(s) | 52, 408, 719, 745, 799, 1234 |
| AlwI | GGATC | 3 cut(s) | 836, 849, 949 |
| AoxI | GGCC | 6 cut(s) | 3, 157, 260, 568, 847, 987 |
| ApeKI | GCWGC | 3 cut(s) | 705, 884, 1070 |
| ApoI | RAATTY | 1 cut(s) | 301 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 458, 490 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 305 |
| AspA2I | CCTAGG | 1 cut(s) | 1190 |
| AspLEI | GCGC | 3 cut(s) | 30, 199, 1313 |
| AspS9I | GGNCC | 5 cut(s) | 261, 454, 466, 568, 848 |
| AsuC2I | CCSGG | 1 cut(s) | 637 |
| AsuHPI | GGTGA | 2 cut(s) | 825, 961 |
| AsuII | TTCGAA | 1 cut(s) | 504 |
| AvaII | GGWCC | 2 cut(s) | 454, 466 |
| AvrII | CCTAGG | 1 cut(s) | 1190 |
| BaeGI | GKGCMC | 1 cut(s) | 362 |
| BamHI | GGATCC | 1 cut(s) | 841 |
| BanI | GGYRCC | 1 cut(s) | 359 |
| BanII | GRGCYC | 1 cut(s) | 384 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 727, 759 |
| BbvI | GCAGC | 3 cut(s) | 717, 871, 1082 |
| BccI | CCATC | 5 cut(s) | 371, 427, 917, 966, 1016 |
| BciT130I | CCWGG | 3 cut(s) | 580, 846, 1041 |
| BciVI | GTATCC | 1 cut(s) | 576 |
| BclI | TGATCA | 1 cut(s) | 934 |
| BcnI | CCSGG | 1 cut(s) | 637 |
| BcoDI | GTCTC | 6 cut(s) | 52, 408, 719, 745, 799, 1234 |
| BfaI | CTAG | 3 cut(s) | 783, 795, 1191 |
| BfrI | CTTAAG | 1 cut(s) | 248 |
| BfuAI | ACCTGC | 1 cut(s) | 1157 |
| BfuI | GTATCC | 1 cut(s) | 576 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 379 |
| BisI | GCNGC | 5 cut(s) | 706, 870, 885, 1068, 1071 |
| BlnI | CCTAGG | 1 cut(s) | 1190 |
| BlpI | GCTNAGC | 1 cut(s) | 606 |
| BlsI | GCNGC | 5 cut(s) | 707, 871, 886, 1069, 1072 |
| Bme1390I | CCNGG | 4 cut(s) | 580, 637, 846, 1041 |
| Bme18I | GGWCC | 2 cut(s) | 454, 466 |
| BmgBI | CACGTC | 1 cut(s) | 773 |
| BmgT120I | GGNCC | 5 cut(s) | 261, 454, 466, 568, 848 |
| BmiI | GGNNCC | 7 cut(s) | 361, 383, 455, 467, 843, 984, 1330 |
| BmrFI | CCNGG | 4 cut(s) | 580, 637, 846, 1041 |
| BmsI | GCATC | 1 cut(s) | 1345 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1139, 1171 |
| BpmI | CTGGAG | 2 cut(s) | 84, 299 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 606 |
| Bpu14I | TTCGAA | 1 cut(s) | 504 |
| BpuMI | CCSGG | 1 cut(s) | 637 |
| BsaAI | YACGTR | 1 cut(s) | 1292 |
| BsaHI | GRCGYC | 1 cut(s) | 808 |
| BsaI | GGTCTC | 2 cut(s) | 408, 745 |
| BsaJI | CCNNGG | 6 cut(s) | 292, 469, 837, 1040, 1107, 1190 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 716, 746, 1004, 1034 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 463, 565, 1304 |
| Bse118I | RCCGGY | 1 cut(s) | 985 |
| Bse1I | ACTGG | 5 cut(s) | 87, 101, 316, 917, 1309 |
| BseBI | CCWGG | 3 cut(s) | 580, 846, 1041 |
| BseDI | CCNNGG | 6 cut(s) | 292, 469, 837, 1040, 1107, 1190 |
| BseGI | GGATG | 3 cut(s) | 91, 419, 1008 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 463, 565, 1304 |
| BseMII | CTCAG | 2 cut(s) | 620, 849 |
| BseNI | ACTGG | 5 cut(s) | 87, 101, 316, 917, 1309 |
| BseRI | GAGGAG | 6 cut(s) | 374, 817, 1009, 1012, 1081, 1331 |
| BseSI | GKGCMC | 1 cut(s) | 362 |
| BseXI | GCAGC | 3 cut(s) | 717, 871, 1082 |
| Bsh1236I | CGCG | 2 cut(s) | 197, 1311 |
| BshFI | GGCC | 6 cut(s) | 5, 159, 262, 570, 849, 989 |
| BshNI | GGYRCC | 1 cut(s) | 359 |
| BsiSI | CCGG | 3 cut(s) | 637, 980, 986 |
| BslFI | GGGAC | 1 cut(s) | 479 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 463, 565, 1304 |
| BsmAI | GTCTC | 6 cut(s) | 52, 408, 719, 745, 799, 1234 |
| BsmBI | CGTCTC | 3 cut(s) | 52, 799, 1234 |
| BsmFI | GGGAC | 1 cut(s) | 479 |
| BsmI | GAATGC | 1 cut(s) | 1032 |
| BsnI | GGCC | 6 cut(s) | 5, 159, 262, 570, 849, 989 |
| Bso31I | GGTCTC | 2 cut(s) | 408, 745 |
| Bsp119I | TTCGAA | 1 cut(s) | 504 |
| Bsp1286I | GDGCHC | 2 cut(s) | 362, 384 |
| Bsp143I | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 606 |
| BspACI | CCGC | 8 cut(s) | 151, 388, 457, 559, 870, 950, 977, 1067 |
| BspANI | GGCC | 6 cut(s) | 5, 159, 262, 570, 849, 989 |
| BspCNI | CTCAG | 2 cut(s) | 619, 850 |
| BspFNI | CGCG | 2 cut(s) | 197, 1311 |
| BspLI | GGNNCC | 7 cut(s) | 361, 383, 455, 467, 843, 984, 1330 |
| BspMI | ACCTGC | 1 cut(s) | 1157 |
| BspPI | GGATC | 3 cut(s) | 836, 849, 949 |
| BspT104I | TTCGAA | 1 cut(s) | 504 |
| BspT107I | GGYRCC | 1 cut(s) | 359 |
| BspTI | CTTAAG | 1 cut(s) | 248 |
| BspTNI | GGTCTC | 2 cut(s) | 408, 745 |
| BsrBI | CCGCTC | 2 cut(s) | 561, 872 |
| BsrFI | RCCGGY | 1 cut(s) | 985 |
| BsrI | ACTGG | 5 cut(s) | 87, 101, 316, 917, 1309 |
| BssAI | RCCGGY | 1 cut(s) | 985 |
| BssECI | CCNNGG | 6 cut(s) | 292, 469, 837, 1040, 1107, 1190 |
| BssMI | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| BssNAI | GTATAC | 1 cut(s) | 926 |
| BssNI | GRCGYC | 1 cut(s) | 808 |
| BssT1I | CCWWGG | 3 cut(s) | 292, 469, 1190 |
| Bst1107I | GTATAC | 1 cut(s) | 926 |
| Bst2UI | CCWGG | 3 cut(s) | 580, 846, 1041 |
| Bst4CI | ACNGT | 3 cut(s) | 75, 553, 1251 |
| Bst6I | CTCTTC | 6 cut(s) | 55, 105, 748, 822, 1191, 1290 |
| BstACI | GRCGYC | 1 cut(s) | 808 |
| BstAFI | CTTAAG | 1 cut(s) | 248 |
| BstBAI | YACGTR | 1 cut(s) | 1292 |
| BstBI | TTCGAA | 1 cut(s) | 504 |
| BstC8I | GCNNGC | 3 cut(s) | 527, 987, 1131 |
| BstDEI | CTNAG | 3 cut(s) | 606, 858, 1299 |
| BstDSI | CCRYGG | 1 cut(s) | 837 |
| BstF5I | GGATG | 3 cut(s) | 91, 419, 1008 |
| BstFNI | CGCG | 2 cut(s) | 197, 1311 |
| BstHHI | GCGC | 3 cut(s) | 30, 199, 1313 |
| BstKTI | GATC | 5 cut(s) | 91, 166, 844, 937, 957 |
| BstMAI | GTCTC | 6 cut(s) | 52, 408, 719, 745, 799, 1234 |
| BstMBI | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 379, 567, 875, 884, 1139 |
| BstNI | CCWGG | 3 cut(s) | 580, 846, 1041 |
| BstNSI | RCATGY | 2 cut(s) | 529, 1129 |
| BstSCI | CCNGG | 4 cut(s) | 578, 635, 844, 1039 |
| BstSLI | GKGCMC | 1 cut(s) | 362 |
| BstUI | CGCG | 2 cut(s) | 197, 1311 |
| BstV1I | GCAGC | 3 cut(s) | 717, 871, 1082 |
| BstX2I | RGATCY | 1 cut(s) | 841 |
| BstYI | RGATCY | 1 cut(s) | 841 |
| BstZ17I | GTATAC | 1 cut(s) | 926 |
| BsuI | GTATCC | 1 cut(s) | 576 |
| BsuRI | GGCC | 6 cut(s) | 5, 159, 262, 570, 849, 989 |
| BtgI | CCRYGG | 1 cut(s) | 837 |
| BtrI | CACGTC | 1 cut(s) | 773 |
| BtsCI | GGATG | 3 cut(s) | 91, 419, 1008 |
| BtsIMutI | CAGTG | 2 cut(s) | 80, 753 |
| BveI | ACCTGC | 1 cut(s) | 1157 |
| Cac8I | GCNNGC | 3 cut(s) | 527, 987, 1131 |
| CfoI | GCGC | 3 cut(s) | 30, 199, 1313 |
| Cfr10I | RCCGGY | 1 cut(s) | 985 |
| Cfr13I | GGNCC | 5 cut(s) | 261, 454, 466, 568, 848 |
| CseI | GACGC | 1 cut(s) | 816 |
| Csp6I | GTAC | 1 cut(s) | 1002 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1084, 1119 |
| CviAII | CATG | 8 cut(s) | 310, 526, 653, 827, 881, 889, 1077, 1126 |
| CviQI | GTAC | 1 cut(s) | 1002 |
| DdeI | CTNAG | 3 cut(s) | 606, 858, 1299 |
| DpnI | GATC | 5 cut(s) | 90, 165, 843, 936, 956 |
| DpnII | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| DraI | TTTAAA | 1 cut(s) | 1266 |
| EaeI | YGGCCR | 1 cut(s) | 987 |
| Eam1104I | CTCTTC | 6 cut(s) | 55, 105, 748, 822, 1191, 1290 |
| EarI | CTCTTC | 6 cut(s) | 55, 105, 748, 822, 1191, 1290 |
| EciI | GGCGGA | 2 cut(s) | 939, 966 |
| Eco130I | CCWWGG | 3 cut(s) | 292, 469, 1190 |
| Eco147I | AGGCCT | 1 cut(s) | 159 |
| Eco24I | GRGCYC | 1 cut(s) | 384 |
| Eco31I | GGTCTC | 2 cut(s) | 408, 745 |
| Eco47I | GGWCC | 2 cut(s) | 454, 466 |
| Eco57I | CTGAAG | 1 cut(s) | 729 |
| EcoO109I | RGGNCCY | 2 cut(s) | 466, 848 |
| EcoRII | CCWGG | 3 cut(s) | 578, 844, 1039 |
| EcoT14I | CCWWGG | 3 cut(s) | 292, 469, 1190 |
| EcoT38I | GRGCYC | 1 cut(s) | 384 |
| ErhI | CCWWGG | 3 cut(s) | 292, 469, 1190 |
| Esp3I | CGTCTC | 3 cut(s) | 52, 799, 1234 |
| FaeI | CATG | 8 cut(s) | 313, 529, 656, 830, 884, 892, 1080, 1129 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 290, 322, 1167, 1199 |
| FaqI | GGGAC | 1 cut(s) | 479 |
| FatI | CATG | 8 cut(s) | 309, 525, 652, 826, 880, 888, 1076, 1125 |
| FbaI | TGATCA | 1 cut(s) | 934 |
| FblI | GTMKAC | 1 cut(s) | 925 |
| Fnu4HI | GCNGC | 5 cut(s) | 706, 870, 885, 1068, 1071 |
| FokI | GGATG | 3 cut(s) | 98, 406, 995 |
| FriOI | GRGCYC | 1 cut(s) | 384 |
| Fsp4HI | GCNGC | 5 cut(s) | 706, 870, 885, 1068, 1071 |
| FspBI | CTAG | 3 cut(s) | 783, 795, 1191 |
| GlaI | GCGC | 3 cut(s) | 29, 198, 1312 |
| GluI | GCNGC | 5 cut(s) | 706, 870, 885, 1068, 1071 |
| GsuI | CTGGAG | 2 cut(s) | 84, 299 |
| HaeIII | GGCC | 6 cut(s) | 5, 159, 262, 570, 849, 989 |
| HapII | CCGG | 3 cut(s) | 637, 980, 986 |
| HgaI | GACGC | 1 cut(s) | 816 |
| HhaI | GCGC | 3 cut(s) | 30, 199, 1313 |
| Hin1I | GRCGYC | 1 cut(s) | 808 |
| Hin1II | CATG | 8 cut(s) | 313, 529, 656, 830, 884, 892, 1080, 1129 |
| Hin6I | GCGC | 3 cut(s) | 28, 197, 1311 |
| HinP1I | GCGC | 3 cut(s) | 28, 197, 1311 |
| HincII | GTYRAC | 2 cut(s) | 556, 1117 |
| HindII | GTYRAC | 2 cut(s) | 556, 1117 |
| HinfI | GANTC | 6 cut(s) | 97, 677, 787, 830, 966, 1057 |
| HpaII | CCGG | 3 cut(s) | 637, 980, 986 |
| HphI | GGTGA | 2 cut(s) | 825, 961 |
| Hpy166II | GTNNAC | 5 cut(s) | 556, 926, 1082, 1117, 1218 |
| Hpy188III | TCNNGA | 4 cut(s) | 94, 1231, 1272, 1318 |
| Hpy8I | GTNNAC | 5 cut(s) | 556, 926, 1082, 1117, 1218 |
| Hpy99I | CGWCG | 1 cut(s) | 774 |
| HpyAV | CCTTC | 3 cut(s) | 202, 1145, 1202 |
| HpyCH4III | ACNGT | 3 cut(s) | 75, 553, 1251 |
| HpyCH4IV | ACGT | 2 cut(s) | 772, 1291 |
| HpyCH4V | TGCA | 4 cut(s) | 171, 340, 443, 705 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 379, 567, 875, 884, 1139 |
| HpyF3I | CTNAG | 3 cut(s) | 606, 858, 1299 |
| HpySE526I | ACGT | 2 cut(s) | 772, 1291 |
| Hsp92I | GRCGYC | 1 cut(s) | 808 |
| Hsp92II | CATG | 8 cut(s) | 313, 529, 656, 830, 884, 892, 1080, 1129 |
| HspAI | GCGC | 3 cut(s) | 28, 197, 1311 |
| KroI | GCCGGC | 1 cut(s) | 985 |
| KroNI | GCCGGC | 1 cut(s) | 987 |
| Ksp22I | TGATCA | 1 cut(s) | 934 |
| Kzo9I | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| LmnI | GCTCC | 5 cut(s) | 318, 387, 877, 982, 1328 |
| Lsp1109I | GCAGC | 3 cut(s) | 717, 871, 1082 |
| LweI | GCATC | 1 cut(s) | 1345 |
| MaeI | CTAG | 3 cut(s) | 783, 795, 1191 |
| MaeII | ACGT | 2 cut(s) | 772, 1291 |
| MaeIII | GTNAC | 2 cut(s) | 631, 967 |
| MalI | GATC | 5 cut(s) | 90, 165, 843, 936, 956 |
| MbiI | CCGCTC | 2 cut(s) | 561, 872 |
| MboI | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| MflI | RGATCY | 1 cut(s) | 841 |
| MhlI | GDGCHC | 2 cut(s) | 362, 384 |
| MluCI | AATT | 2 cut(s) | 301, 507 |
| MlyI | GAGTC | 3 cut(s) | 91, 686, 975 |
| MroNI | GCCGGC | 1 cut(s) | 985 |
| MroXI | GAANNNNTTC | 1 cut(s) | 305 |
| MseI | TTAA | 5 cut(s) | 249, 392, 1253, 1265, 1348 |
| MslI | CAYNNNNRTG | 1 cut(s) | 919 |
| MspCI | CTTAAG | 1 cut(s) | 248 |
| MspI | CCGG | 3 cut(s) | 637, 980, 986 |
| MspR9I | CCNGG | 4 cut(s) | 580, 637, 846, 1041 |
| Mva1269I | GAATGC | 1 cut(s) | 1032 |
| MvaI | CCWGG | 3 cut(s) | 580, 846, 1041 |
| MvnI | CGCG | 2 cut(s) | 197, 1311 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 379, 567, 875, 884, 1139 |
| NaeI | GCCGGC | 1 cut(s) | 987 |
| NciI | CCSGG | 1 cut(s) | 637 |
| NdeII | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| NgoMIV | GCCGGC | 1 cut(s) | 985 |
| NlaIII | CATG | 8 cut(s) | 313, 529, 656, 830, 884, 892, 1080, 1129 |
| NlaIV | GGNNCC | 7 cut(s) | 361, 383, 455, 467, 843, 984, 1330 |
| NmuCI | GTSAC | 2 cut(s) | 631, 967 |
| NspI | RCATGY | 2 cut(s) | 529, 1129 |
| NspV | TTCGAA | 1 cut(s) | 504 |
| PaeI | GCATGC | 1 cut(s) | 529 |
| PaqCI | CACCTGC | 1 cut(s) | 1157 |
| PceI | AGGCCT | 1 cut(s) | 159 |
| PctI | GAATGC | 1 cut(s) | 1032 |
| PdiI | GCCGGC | 1 cut(s) | 987 |
| PdmI | GAANNNNTTC | 1 cut(s) | 305 |
| PfeI | GAWTC | 3 cut(s) | 787, 830, 1057 |
| PflFI | GACNNNGTC | 1 cut(s) | 637 |
| PkrI | GCNGC | 5 cut(s) | 707, 871, 886, 1069, 1072 |
| PleI | GAGTC | 3 cut(s) | 91, 685, 974 |
| PpsI | GAGTC | 3 cut(s) | 91, 685, 974 |
| Ppu21I | YACGTR | 1 cut(s) | 1292 |
| PpuMI | RGGWCCY | 1 cut(s) | 466 |
| Psp5II | RGGWCCY | 1 cut(s) | 466 |
| Psp6I | CCWGG | 3 cut(s) | 578, 844, 1039 |
| PspGI | CCWGG | 3 cut(s) | 578, 844, 1039 |
| PspN4I | GGNNCC | 7 cut(s) | 361, 383, 455, 467, 843, 984, 1330 |
| PspPI | GGNCC | 5 cut(s) | 261, 454, 466, 568, 848 |
| PspPPI | RGGWCCY | 1 cut(s) | 466 |
| PsuI | RGATCY | 1 cut(s) | 841 |
| PsyI | GACNNNGTC | 1 cut(s) | 637 |
| RsaI | GTAC | 1 cut(s) | 1003 |
| RsaNI | GTAC | 1 cut(s) | 1002 |
| RseI | CAYNNNNRTG | 1 cut(s) | 919 |
| SaqAI | TTAA | 5 cut(s) | 249, 392, 1253, 1265, 1348 |
| SatI | GCNGC | 5 cut(s) | 706, 870, 885, 1068, 1071 |
| Sau3AI | GATC | 5 cut(s) | 88, 163, 841, 934, 954 |
| Sau96I | GGNCC | 5 cut(s) | 261, 454, 466, 568, 848 |
| SchI | GAGTC | 3 cut(s) | 91, 686, 975 |
| ScrFI | CCNGG | 4 cut(s) | 580, 637, 846, 1041 |
| SduI | GDGCHC | 2 cut(s) | 362, 384 |
| SfaNI | GCATC | 1 cut(s) | 1345 |
| SfuI | TTCGAA | 1 cut(s) | 504 |
| SinI | GGWCC | 2 cut(s) | 454, 466 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 919 |
| SmlI | CTYRAG | 1 cut(s) | 248 |
| SmoI | CTYRAG | 1 cut(s) | 248 |
| SphI | GCATGC | 1 cut(s) | 529 |
| Sse9I | AATT | 2 cut(s) | 301, 507 |
| SseBI | AGGCCT | 1 cut(s) | 159 |
| SsiI | CCGC | 8 cut(s) | 151, 388, 457, 559, 870, 950, 977, 1067 |
| SspMI | CTAG | 3 cut(s) | 783, 795, 1191 |
| StuI | AGGCCT | 1 cut(s) | 159 |
| StyD4I | CCNGG | 4 cut(s) | 578, 635, 844, 1039 |
| StyI | CCWWGG | 3 cut(s) | 292, 469, 1190 |
| TaaI | ACNGT | 3 cut(s) | 75, 553, 1251 |
| TaiI | ACGT | 2 cut(s) | 775, 1294 |
| TaqI | TCGA | 5 cut(s) | 330, 504, 769, 1232, 1271 |
| TasI | AATT | 2 cut(s) | 301, 507 |
| TauI | GCSGC | 2 cut(s) | 872, 1070 |
| TfiI | GAWTC | 3 cut(s) | 787, 830, 1057 |
| Tru1I | TTAA | 5 cut(s) | 249, 392, 1253, 1265, 1348 |
| Tru9I | TTAA | 5 cut(s) | 249, 392, 1253, 1265, 1348 |
| TscAI | CASTG | 2 cut(s) | 87, 753 |
| TseFI | GTSAC | 2 cut(s) | 631, 967 |
| TseI | GCWGC | 3 cut(s) | 705, 884, 1070 |
| Tsp45I | GTSAC | 2 cut(s) | 631, 967 |
| TspDTI | ATGAA | 4 cut(s) | 156, 298, 779, 986 |
| TspGWI | ACGGA | 3 cut(s) | 660, 814, 854 |
| TspRI | CASTG | 2 cut(s) | 87, 753 |
| Tth111I | GACNNNGTC | 1 cut(s) | 637 |
| Vha464I | CTTAAG | 1 cut(s) | 248 |
| VpaK11BI | GGWCC | 2 cut(s) | 454, 466 |
| XapI | RAATTY | 1 cut(s) | 301 |
| XceI | RCATGY | 2 cut(s) | 529, 1129 |
| XmaJI | CCTAGG | 1 cut(s) | 1190 |
| XmiI | GTMKAC | 1 cut(s) | 925 |
| XmnI | GAANNNNTTC | 1 cut(s) | 305 |
| XspI | CTAG | 3 cut(s) | 783, 795, 1191 |