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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 418 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 19 |
| AccBSI | CCGCTC | 1 cut(s) | 40 |
| AciI | CCGC | 4 cut(s) | 40, 173, 368, 461 |
| AcuI | CTGAAG | 1 cut(s) | 89 |
| AdeI | CACNNNGTG | 1 cut(s) | 19 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 19 |
| AflII | CTTAAG | 1 cut(s) | 196 |
| AgsI | TTSAA | 2 cut(s) | 238, 354 |
| AjnI | CCWGG | 2 cut(s) | 15, 370 |
| AluBI | AGCT | 3 cut(s) | 287, 417, 536 |
| AluI | AGCT | 3 cut(s) | 287, 417, 536 |
| Alw26I | GTCTC | 1 cut(s) | 130 |
| Ama87I | CYCGRG | 1 cut(s) | 493 |
| ApeKI | GCWGC | 2 cut(s) | 23, 536 |
| AseI | ATTAAT | 1 cut(s) | 467 |
| AsuC2I | CCSGG | 2 cut(s) | 494, 495 |
| AsuHPI | GGTGA | 2 cut(s) | 219, 331 |
| AsuNHI | GCTAGC | 1 cut(s) | 413 |
| AvaI | CYCGRG | 1 cut(s) | 493 |
| BanII | GRGCYC | 1 cut(s) | 500 |
| BbvI | GCAGC | 2 cut(s) | 10, 523 |
| BccI | CCATC | 3 cut(s) | 214, 274, 379 |
| BciT130I | CCWGG | 2 cut(s) | 17, 372 |
| BcnI | CCSGG | 2 cut(s) | 494, 495 |
| BcoDI | GTCTC | 1 cut(s) | 130 |
| BfaI | CTAG | 2 cut(s) | 284, 414 |
| BfrI | CTTAAG | 1 cut(s) | 196 |
| BfuAI | ACCTGC | 1 cut(s) | 418 |
| BisI | GCNGC | 2 cut(s) | 24, 537 |
| BlsI | GCNGC | 2 cut(s) | 25, 538 |
| Bme1390I | CCNGG | 4 cut(s) | 17, 372, 494, 495 |
| BmeT110I | CYCGRG | 1 cut(s) | 493 |
| BmiI | GGNNCC | 1 cut(s) | 499 |
| BmrFI | CCNGG | 4 cut(s) | 17, 372, 494, 495 |
| BmsI | GCATC | 1 cut(s) | 312 |
| BmtI | GCTAGC | 1 cut(s) | 417 |
| BpmI | CTGGAG | 2 cut(s) | 285, 527 |
| BpuMI | CCSGG | 2 cut(s) | 494, 495 |
| BsaI | GGTCTC | 1 cut(s) | 130 |
| BsaJI | CCNNGG | 1 cut(s) | 493 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 283, 313, 412, 442 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 19 |
| Bse118I | RCCGGY | 3 cut(s) | 175, 316, 334 |
| Bse1I | ACTGG | 2 cut(s) | 140, 302 |
| BseBI | CCWGG | 2 cut(s) | 17, 372 |
| BseDI | CCNNGG | 1 cut(s) | 493 |
| BseGI | GGATG | 2 cut(s) | 316, 390 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 19 |
| BseNI | ACTGG | 2 cut(s) | 140, 302 |
| BseXI | GCAGC | 2 cut(s) | 10, 523 |
| BseYI | CCCAGC | 1 cut(s) | 256 |
| BsiHKCI | CYCGRG | 1 cut(s) | 493 |
| BsiSI | CCGG | 4 cut(s) | 176, 317, 335, 494 |
| BslFI | GGGAC | 1 cut(s) | 350 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 19 |
| BsmAI | GTCTC | 1 cut(s) | 130 |
| BsmFI | GGGAC | 1 cut(s) | 350 |
| Bso31I | GGTCTC | 1 cut(s) | 130 |
| BsoBI | CYCGRG | 1 cut(s) | 493 |
| Bsp1286I | GDGCHC | 1 cut(s) | 500 |
| Bsp143I | GATC | 1 cut(s) | 400 |
| BspACI | CCGC | 4 cut(s) | 40, 173, 368, 461 |
| BspLI | GGNNCC | 1 cut(s) | 499 |
| BspMI | ACCTGC | 1 cut(s) | 418 |
| BspOI | GCTAGC | 1 cut(s) | 417 |
| BspTI | CTTAAG | 1 cut(s) | 196 |
| BspTNI | GGTCTC | 1 cut(s) | 130 |
| BsrBI | CCGCTC | 1 cut(s) | 40 |
| BsrFI | RCCGGY | 3 cut(s) | 175, 316, 334 |
| BsrI | ACTGG | 2 cut(s) | 140, 302 |
| BssAI | RCCGGY | 3 cut(s) | 175, 316, 334 |
| BssECI | CCNNGG | 1 cut(s) | 493 |
| BssMI | GATC | 1 cut(s) | 400 |
| Bst2UI | CCWGG | 2 cut(s) | 17, 372 |
| Bst6I | CTCTTC | 2 cut(s) | 65, 459 |
| BstAFI | CTTAAG | 1 cut(s) | 196 |
| BstC8I | GCNNGC | 2 cut(s) | 177, 415 |
| BstF5I | GGATG | 2 cut(s) | 316, 390 |
| BstKTI | GATC | 1 cut(s) | 403 |
| BstMAI | GTCTC | 1 cut(s) | 130 |
| BstMBI | GATC | 1 cut(s) | 400 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 284, 293, 322, 331 |
| BstNI | CCWGG | 2 cut(s) | 17, 372 |
| BstSCI | CCNGG | 4 cut(s) | 15, 370, 492, 493 |
| BstV1I | GCAGC | 2 cut(s) | 10, 523 |
| BtsCI | GGATG | 2 cut(s) | 316, 390 |
| BtsI | GCAGTG | 2 cut(s) | 273, 291 |
| BtsIMutI | CAGTG | 3 cut(s) | 273, 291, 490 |
| BveI | ACCTGC | 1 cut(s) | 418 |
| Cac8I | GCNNGC | 2 cut(s) | 177, 415 |
| Cfr10I | RCCGGY | 3 cut(s) | 175, 316, 334 |
| Cfr9I | CCCGGG | 1 cut(s) | 493 |
| CseI | GACGC | 1 cut(s) | 352 |
| CsiI | ACCWGGT | 1 cut(s) | 15 |
| CviAII | CATG | 2 cut(s) | 119, 433 |
| CviJI | RGCY | 8 cut(s) | 23, 149, 224, 287, 316, 417, 498, 536 |
| CviKI_1 | RGCY | 8 cut(s) | 23, 149, 224, 287, 316, 417, 498, 536 |
| DpnI | GATC | 1 cut(s) | 402 |
| DpnII | GATC | 1 cut(s) | 400 |
| DraIII | CACNNNGTG | 1 cut(s) | 19 |
| Eam1104I | CTCTTC | 2 cut(s) | 65, 459 |
| EarI | CTCTTC | 2 cut(s) | 65, 459 |
| Eco24I | GRGCYC | 1 cut(s) | 500 |
| Eco31I | GGTCTC | 1 cut(s) | 130 |
| Eco57I | CTGAAG | 1 cut(s) | 89 |
| Eco88I | CYCGRG | 1 cut(s) | 493 |
| EcoRII | CCWGG | 2 cut(s) | 15, 370 |
| EcoT38I | GRGCYC | 1 cut(s) | 500 |
| FaeI | CATG | 2 cut(s) | 122, 436 |
| FaiI | YATR | 2 cut(s) | 120, 434 |
| FaqI | GGGAC | 1 cut(s) | 350 |
| FatI | CATG | 2 cut(s) | 118, 432 |
| FauI | CCCGC | 3 cut(s) | 33, 375, 454 |
| Fnu4HI | GCNGC | 2 cut(s) | 24, 537 |
| FokI | GGATG | 2 cut(s) | 323, 397 |
| FriOI | GRGCYC | 1 cut(s) | 500 |
| Fsp4HI | GCNGC | 2 cut(s) | 24, 537 |
| FspBI | CTAG | 2 cut(s) | 284, 414 |
| GluI | GCNGC | 2 cut(s) | 24, 537 |
| GsaI | CCCAGC | 1 cut(s) | 260 |
| GsuI | CTGGAG | 2 cut(s) | 285, 527 |
| HapII | CCGG | 4 cut(s) | 176, 317, 335, 494 |
| HgaI | GACGC | 1 cut(s) | 352 |
| Hin1II | CATG | 2 cut(s) | 122, 436 |
| HincII | GTYRAC | 2 cut(s) | 51, 426 |
| HindII | GTYRAC | 2 cut(s) | 51, 426 |
| HinfI | GANTC | 2 cut(s) | 5, 350 |
| HpaII | CCGG | 4 cut(s) | 176, 317, 335, 494 |
| HphI | GGTGA | 2 cut(s) | 219, 331 |
| Hpy166II | GTNNAC | 3 cut(s) | 13, 51, 426 |
| Hpy188I | TCNGA | 4 cut(s) | 64, 146, 349, 384 |
| Hpy188III | TCNNGA | 2 cut(s) | 247, 506 |
| Hpy8I | GTNNAC | 3 cut(s) | 13, 51, 426 |
| Hpy99I | CGWCG | 1 cut(s) | 209 |
| HpyAV | CCTTC | 2 cut(s) | 39, 69 |
| HpyCH4IV | ACGT | 2 cut(s) | 207, 422 |
| HpyCH4V | TGCA | 3 cut(s) | 128, 278, 340 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 284, 293, 322, 331 |
| HpySE526I | ACGT | 2 cut(s) | 207, 422 |
| Hsp92II | CATG | 2 cut(s) | 122, 436 |
| KroI | GCCGGC | 1 cut(s) | 175 |
| KroNI | GCCGGC | 1 cut(s) | 177 |
| Kzo9I | GATC | 1 cut(s) | 400 |
| LmnI | GCTCC | 1 cut(s) | 503 |
| Lsp1109I | GCAGC | 2 cut(s) | 10, 523 |
| LweI | GCATC | 1 cut(s) | 312 |
| MabI | ACCWGGT | 1 cut(s) | 15 |
| MaeI | CTAG | 2 cut(s) | 284, 414 |
| MaeII | ACGT | 2 cut(s) | 207, 422 |
| MalI | GATC | 1 cut(s) | 402 |
| MbiI | CCGCTC | 1 cut(s) | 40 |
| MboI | GATC | 1 cut(s) | 400 |
| MboII | GAAGA | 2 cut(s) | 82, 446 |
| MhlI | GDGCHC | 1 cut(s) | 500 |
| MluCI | AATT | 4 cut(s) | 129, 279, 468, 515 |
| MmeI | TCCRAC | 1 cut(s) | 430 |
| MnlI | CCTC | 1 cut(s) | 72 |
| MroNI | GCCGGC | 1 cut(s) | 175 |
| MseI | TTAA | 3 cut(s) | 197, 467, 479 |
| MslI | CAYNNNNRTG | 1 cut(s) | 123 |
| MspCI | CTTAAG | 1 cut(s) | 196 |
| MspI | CCGG | 4 cut(s) | 176, 317, 335, 494 |
| MspR9I | CCNGG | 4 cut(s) | 17, 372, 494, 495 |
| MvaI | CCWGG | 2 cut(s) | 17, 372 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 284, 293, 322, 331 |
| NaeI | GCCGGC | 1 cut(s) | 177 |
| NciI | CCSGG | 2 cut(s) | 494, 495 |
| NdeII | GATC | 1 cut(s) | 400 |
| NgoMIV | GCCGGC | 1 cut(s) | 175 |
| NheI | GCTAGC | 1 cut(s) | 413 |
| NlaIII | CATG | 2 cut(s) | 122, 436 |
| NlaIV | GGNNCC | 1 cut(s) | 499 |
| PdiI | GCCGGC | 1 cut(s) | 177 |
| PfeI | GAWTC | 2 cut(s) | 5, 350 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 19 |
| PkrI | GCNGC | 2 cut(s) | 25, 538 |
| PshBI | ATTAAT | 1 cut(s) | 467 |
| Psp6I | CCWGG | 2 cut(s) | 15, 370 |
| PspFI | CCCAGC | 1 cut(s) | 256 |
| PspGI | CCWGG | 2 cut(s) | 15, 370 |
| PspN4I | GGNNCC | 1 cut(s) | 499 |
| RseI | CAYNNNNRTG | 1 cut(s) | 123 |
| SaqAI | TTAA | 3 cut(s) | 197, 467, 479 |
| SatI | GCNGC | 2 cut(s) | 24, 537 |
| Sau3AI | GATC | 1 cut(s) | 400 |
| ScrFI | CCNGG | 4 cut(s) | 17, 372, 494, 495 |
| SduI | GDGCHC | 1 cut(s) | 500 |
| SexAI | ACCWGGT | 1 cut(s) | 15 |
| SfaNI | GCATC | 1 cut(s) | 312 |
| SmaI | CCCGGG | 1 cut(s) | 495 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 123 |
| SmlI | CTYRAG | 1 cut(s) | 196 |
| SmoI | CTYRAG | 1 cut(s) | 196 |
| Sse9I | AATT | 4 cut(s) | 129, 279, 468, 515 |
| SsiI | CCGC | 4 cut(s) | 40, 173, 368, 461 |
| SspMI | CTAG | 2 cut(s) | 284, 414 |
| StyD4I | CCNGG | 4 cut(s) | 15, 370, 492, 493 |
| TaiI | ACGT | 2 cut(s) | 210, 425 |
| TaqI | TCGA | 2 cut(s) | 88, 246 |
| TasI | AATT | 4 cut(s) | 129, 279, 468, 515 |
| TfiI | GAWTC | 2 cut(s) | 5, 350 |
| Tru1I | TTAA | 3 cut(s) | 197, 467, 479 |
| Tru9I | TTAA | 3 cut(s) | 197, 467, 479 |
| TscAI | CASTG | 3 cut(s) | 280, 298, 490 |
| TseI | GCWGC | 2 cut(s) | 23, 536 |
| TspGWI | ACGGA | 1 cut(s) | 407 |
| TspMI | CCCGGG | 1 cut(s) | 493 |
| TspRI | CASTG | 3 cut(s) | 280, 298, 490 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 19 |
| Vha464I | CTTAAG | 1 cut(s) | 196 |
| VspI | ATTAAT | 1 cut(s) | 467 |
| XmaI | CCCGGG | 1 cut(s) | 493 |
| XspI | CTAG | 2 cut(s) | 284, 414 |