Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 331 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1188 |
| AccI | GTMKAC | 1 cut(s) | 781 |
| AccII | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| AciI | CCGC | 4 cut(s) | 140, 399, 998, 1034 |
| AclWI | GGATC | 5 cut(s) | 207, 272, 797, 810, 1386 |
| AcsI | RAATTY | 4 cut(s) | 103, 426, 698, 1449 |
| AcuI | CTGAAG | 1 cut(s) | 849 |
| AfaI | GTAC | 5 cut(s) | 194, 412, 574, 1225, 1323 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1188, 1487, 1491 |
| AflIII | ACRYGT | 3 cut(s) | 388, 407, 1418 |
| AgsI | TTSAA | 4 cut(s) | 368, 554, 1128, 1512 |
| AhlI | ACTAGT | 1 cut(s) | 1220 |
| AjnI | CCWGG | 2 cut(s) | 195, 463 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1175, 1207 |
| AluBI | AGCT | 6 cut(s) | 301, 596, 744, 893, 973, 1118 |
| AluI | AGCT | 6 cut(s) | 301, 596, 744, 893, 973, 1118 |
| Alw26I | GTCTC | 1 cut(s) | 981 |
| AlwI | GGATC | 5 cut(s) | 207, 272, 797, 810, 1386 |
| Ama87I | CYCGRG | 1 cut(s) | 627 |
| AoxI | GGCC | 4 cut(s) | 514, 609, 866, 1399 |
| ApaI | GGGCCC | 1 cut(s) | 1403 |
| ApeKI | GCWGC | 6 cut(s) | 56, 241, 292, 307, 744, 1073 |
| ApoI | RAATTY | 4 cut(s) | 103, 426, 698, 1449 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 65, 97 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 253 |
| AspLEI | GCGC | 3 cut(s) | 61, 562, 998 |
| AspS9I | GGNCC | 3 cut(s) | 609, 1399, 1400 |
| AsuC2I | CCSGG | 4 cut(s) | 628, 629, 1251, 1262 |
| AsuHPI | GGTGA | 3 cut(s) | 163, 1249, 1268 |
| AvaI | CYCGRG | 1 cut(s) | 627 |
| BaeGI | GKGCMC | 1 cut(s) | 1403 |
| BamHI | GGATCC | 1 cut(s) | 802 |
| BanI | GGYRCC | 1 cut(s) | 331 |
| BanII | GRGCYC | 3 cut(s) | 1289, 1403, 1498 |
| BbsI | GAAGAC | 1 cut(s) | 384 |
| BbvCI | CCTCAGC | 1 cut(s) | 1029 |
| BbvI | GCAGC | 6 cut(s) | 68, 228, 279, 319, 731, 1060 |
| BccI | CCATC | 4 cut(s) | 70, 101, 103, 673 |
| BceAI | ACGGC | 1 cut(s) | 21 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 45, 79, 572, 606, 845, 879 |
| BciT130I | CCWGG | 2 cut(s) | 197, 465 |
| BciVI | GTATCC | 1 cut(s) | 611 |
| BcnI | CCSGG | 4 cut(s) | 628, 629, 1251, 1262 |
| BcoDI | GTCTC | 1 cut(s) | 981 |
| BcuI | ACTAGT | 1 cut(s) | 1220 |
| BfaI | CTAG | 3 cut(s) | 635, 1140, 1221 |
| BfmI | CTRYAG | 1 cut(s) | 879 |
| BfoI | RGCGCY | 1 cut(s) | 62 |
| BfuI | GTATCC | 1 cut(s) | 611 |
| BisI | GCNGC | 8 cut(s) | 57, 140, 242, 293, 308, 745, 1035, 1074 |
| BlsI | GCNGC | 8 cut(s) | 58, 141, 243, 294, 309, 746, 1036, 1075 |
| Bme1390I | CCNGG | 6 cut(s) | 197, 465, 628, 629, 1251, 1262 |
| BmeT110I | CYCGRG | 1 cut(s) | 627 |
| BmgT120I | GGNCC | 3 cut(s) | 609, 1399, 1400 |
| BmiI | GGNNCC | 4 cut(s) | 333, 728, 804, 1401 |
| BmrFI | CCNGG | 6 cut(s) | 197, 465, 628, 629, 1251, 1262 |
| BmsI | GCATC | 5 cut(s) | 28, 39, 544, 553, 842 |
| BpiI | GAAGAC | 1 cut(s) | 384 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1082, 1114 |
| BpmI | CTGGAG | 1 cut(s) | 486 |
| Bpu10I | CCTNAGC | 3 cut(s) | 492, 518, 1029 |
| BpuEI | CTTGAG | 2 cut(s) | 976, 1301 |
| BpuMI | CCSGG | 4 cut(s) | 628, 629, 1251, 1262 |
| BsaAI | YACGTR | 1 cut(s) | 126 |
| BsaBI | GATNNNNATC | 1 cut(s) | 771 |
| BsaJI | CCNNGG | 7 cut(s) | 17, 147, 320, 539, 627, 918, 1260 |
| BsaWI | WCCGGW | 1 cut(s) | 432 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1098, 1128 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1188, 1487, 1491 |
| Bse1I | ACTGG | 1 cut(s) | 658 |
| Bse3DI | GCAATG | 2 cut(s) | 578, 897 |
| Bse8I | GATNNNNATC | 1 cut(s) | 771 |
| BseBI | CCWGG | 2 cut(s) | 197, 465 |
| BseDI | CCNNGG | 7 cut(s) | 17, 147, 320, 539, 627, 918, 1260 |
| BseGI | GGATG | 3 cut(s) | 93, 535, 833 |
| BseJI | GATNNNNATC | 1 cut(s) | 771 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1188, 1487, 1491 |
| BseMI | GCAATG | 2 cut(s) | 578, 897 |
| BseMII | CTCAG | 6 cut(s) | 112, 363, 506, 1020, 1115, 1230 |
| BseNI | ACTGG | 1 cut(s) | 658 |
| BseRI | GAGGAG | 1 cut(s) | 1474 |
| BseSI | GKGCMC | 1 cut(s) | 1403 |
| BseXI | GCAGC | 6 cut(s) | 68, 228, 279, 319, 731, 1060 |
| BsgI | GTGCAG | 2 cut(s) | 278, 891 |
| Bsh1236I | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| BshFI | GGCC | 4 cut(s) | 516, 611, 868, 1401 |
| BshNI | GGYRCC | 1 cut(s) | 331 |
| BsiHKCI | CYCGRG | 1 cut(s) | 627 |
| BsiSI | CCGG | 4 cut(s) | 433, 628, 1251, 1262 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1188, 1487, 1491 |
| BsmAI | GTCTC | 1 cut(s) | 981 |
| BsmI | GAATGC | 1 cut(s) | 970 |
| BsnI | GGCC | 4 cut(s) | 516, 611, 868, 1401 |
| BsoBI | CYCGRG | 1 cut(s) | 627 |
| Bsp120I | GGGCCC | 1 cut(s) | 1399 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1289, 1403, 1498 |
| Bsp1407I | TGTACA | 1 cut(s) | 1321 |
| Bsp143I | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| Bsp19I | CCATGG | 2 cut(s) | 147, 320 |
| BspACI | CCGC | 4 cut(s) | 140, 399, 998, 1034 |
| BspANI | GGCC | 4 cut(s) | 516, 611, 868, 1401 |
| BspCNI | CTCAG | 6 cut(s) | 111, 364, 505, 1021, 1114, 1231 |
| BspFNI | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| BspHI | TCATGA | 1 cut(s) | 763 |
| BspLI | GGNNCC | 4 cut(s) | 333, 728, 804, 1401 |
| BspPI | GGATC | 5 cut(s) | 207, 272, 797, 810, 1386 |
| BspQI | GCTCTTC | 1 cut(s) | 1294 |
| BspT107I | GGYRCC | 1 cut(s) | 331 |
| BsrDI | GCAATG | 2 cut(s) | 578, 897 |
| BsrGI | TGTACA | 1 cut(s) | 1321 |
| BsrI | ACTGG | 1 cut(s) | 658 |
| BssECI | CCNNGG | 7 cut(s) | 17, 147, 320, 539, 627, 918, 1260 |
| BssMI | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| BssT1I | CCWWGG | 4 cut(s) | 17, 147, 320, 539 |
| Bst2UI | CCWGG | 2 cut(s) | 197, 465 |
| Bst4CI | ACNGT | 1 cut(s) | 779 |
| Bst6I | CTCTTC | 4 cut(s) | 666, 1200, 1294, 1513 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 38 |
| BstAUI | TGTACA | 1 cut(s) | 1321 |
| BstBAI | YACGTR | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 489 |
| BstDSI | CCRYGG | 3 cut(s) | 147, 320, 918 |
| BstF5I | GGATG | 3 cut(s) | 93, 535, 833 |
| BstFNI | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| BstH2I | RGCGCY | 1 cut(s) | 62 |
| BstHHI | GCGC | 3 cut(s) | 61, 562, 998 |
| BstKTI | GATC | 7 cut(s) | 202, 280, 592, 805, 930, 1054, 1381 |
| BstMAI | GTCTC | 1 cut(s) | 981 |
| BstMBI | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| BstNI | CCWGG | 2 cut(s) | 197, 465 |
| BstNSI | RCATGY | 4 cut(s) | 385, 411, 491, 1322 |
| BstSCI | CCNGG | 6 cut(s) | 195, 463, 626, 627, 1249, 1260 |
| BstSFI | CTRYAG | 1 cut(s) | 879 |
| BstSLI | GKGCMC | 1 cut(s) | 1403 |
| BstUI | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| BstV1I | GCAGC | 6 cut(s) | 68, 228, 279, 319, 731, 1060 |
| BstV2I | GAAGAC | 1 cut(s) | 384 |
| BstX2I | RGATCY | 1 cut(s) | 802 |
| BstYI | RGATCY | 1 cut(s) | 802 |
| BsuI | GTATCC | 1 cut(s) | 611 |
| BsuRI | GGCC | 4 cut(s) | 516, 611, 868, 1401 |
| BtgI | CCRYGG | 3 cut(s) | 147, 320, 918 |
| BtgZI | GCGATG | 1 cut(s) | 48 |
| BtsCI | GGATG | 3 cut(s) | 93, 535, 833 |
| BtsI | GCAGTG | 1 cut(s) | 449 |
| BtsIMutI | CAGTG | 4 cut(s) | 449, 651, 775, 908 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 489 |
| CciI | TCATGA | 1 cut(s) | 763 |
| CfoI | GCGC | 3 cut(s) | 61, 562, 998 |
| Cfr13I | GGNCC | 3 cut(s) | 609, 1399, 1400 |
| Cfr9I | CCCGGG | 1 cut(s) | 627 |
| CseI | GACGC | 1 cut(s) | 396 |
| Csp6I | GTAC | 5 cut(s) | 193, 411, 573, 1224, 1322 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 981, 1016 |
| CviQI | GTAC | 5 cut(s) | 193, 411, 573, 1224, 1322 |
| DpnI | GATC | 7 cut(s) | 201, 279, 591, 804, 929, 1053, 1380 |
| DpnII | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| DraI | TTTAAA | 1 cut(s) | 460 |
| Eam1104I | CTCTTC | 4 cut(s) | 666, 1200, 1294, 1513 |
| EarI | CTCTTC | 4 cut(s) | 666, 1200, 1294, 1513 |
| EciI | GGCGGA | 1 cut(s) | 414 |
| Eco130I | CCWWGG | 4 cut(s) | 17, 147, 320, 539 |
| Eco24I | GRGCYC | 3 cut(s) | 1289, 1403, 1498 |
| Eco57I | CTGAAG | 1 cut(s) | 849 |
| Eco88I | CYCGRG | 1 cut(s) | 627 |
| EcoO109I | RGGNCCY | 2 cut(s) | 609, 1400 |
| EcoRII | CCWGG | 2 cut(s) | 195, 463 |
| EcoT14I | CCWWGG | 4 cut(s) | 17, 147, 320, 539 |
| EcoT38I | GRGCYC | 3 cut(s) | 1289, 1403, 1498 |
| ErhI | CCWWGG | 4 cut(s) | 17, 147, 320, 539 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 209, 241 |
| FblI | GTMKAC | 1 cut(s) | 781 |
| Fnu4HI | GCNGC | 8 cut(s) | 57, 140, 242, 293, 308, 745, 1035, 1074 |
| FokI | GGATG | 3 cut(s) | 80, 522, 820 |
| FriOI | GRGCYC | 3 cut(s) | 1289, 1403, 1498 |
| Fsp4HI | GCNGC | 8 cut(s) | 57, 140, 242, 293, 308, 745, 1035, 1074 |
| FspBI | CTAG | 3 cut(s) | 635, 1140, 1221 |
| GlaI | GCGC | 3 cut(s) | 60, 561, 997 |
| GluI | GCNGC | 8 cut(s) | 57, 140, 242, 293, 308, 745, 1035, 1074 |
| GsuI | CTGGAG | 1 cut(s) | 486 |
| HaeII | RGCGCY | 1 cut(s) | 62 |
| HaeIII | GGCC | 4 cut(s) | 516, 611, 868, 1401 |
| HapII | CCGG | 4 cut(s) | 433, 628, 1251, 1262 |
| HgaI | GACGC | 1 cut(s) | 396 |
| HhaI | GCGC | 3 cut(s) | 61, 562, 998 |
| Hin6I | GCGC | 3 cut(s) | 59, 560, 996 |
| HinP1I | GCGC | 3 cut(s) | 59, 560, 996 |
| HincII | GTYRAC | 2 cut(s) | 646, 782 |
| HindII | GTYRAC | 2 cut(s) | 646, 782 |
| HindIII | AAGCTT | 1 cut(s) | 299 |
| HinfI | GANTC | 2 cut(s) | 913, 989 |
| HpaII | CCGG | 4 cut(s) | 433, 628, 1251, 1262 |
| HphI | GGTGA | 3 cut(s) | 163, 1249, 1268 |
| Hpy166II | GTNNAC | 3 cut(s) | 646, 782, 1015 |
| Hpy188I | TCNGA | 4 cut(s) | 72, 101, 807, 1470 |
| Hpy188III | TCNNGA | 4 cut(s) | 764, 1140, 1238, 1454 |
| Hpy8I | GTNNAC | 3 cut(s) | 646, 782, 1015 |
| Hpy99I | CGWCG | 1 cut(s) | 390 |
| HpyAV | CCTTC | 4 cut(s) | 67, 337, 683, 1321 |
| HpyCH4III | ACNGT | 1 cut(s) | 779 |
| HpyCH4IV | ACGT | 3 cut(s) | 23, 125, 167 |
| HpyCH4V | TGCA | 9 cut(s) | 267, 295, 583, 848, 908, 947, 968, 1061, 1337 |
| HpySE526I | ACGT | 3 cut(s) | 23, 125, 167 |
| HspAI | GCGC | 3 cut(s) | 59, 560, 996 |
| Kzo9I | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| LguI | GCTCTTC | 1 cut(s) | 1294 |
| LmnI | GCTCC | 4 cut(s) | 46, 732, 898, 1003 |
| Lsp1109I | GCAGC | 6 cut(s) | 68, 228, 279, 319, 731, 1060 |
| LweI | GCATC | 5 cut(s) | 28, 39, 544, 553, 842 |
| MaeI | CTAG | 3 cut(s) | 635, 1140, 1221 |
| MaeII | ACGT | 3 cut(s) | 23, 125, 167 |
| MaeIII | GTNAC | 2 cut(s) | 1274, 1437 |
| MalI | GATC | 7 cut(s) | 201, 279, 591, 804, 929, 1053, 1380 |
| MboI | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| MfeI | CAATTG | 2 cut(s) | 134, 942 |
| MflI | RGATCY | 1 cut(s) | 802 |
| MhlI | GDGCHC | 3 cut(s) | 1289, 1403, 1498 |
| MluI | ACGCGT | 2 cut(s) | 388, 1418 |
| MlyI | GAGTC | 1 cut(s) | 983 |
| MroXI | GAANNNNTTC | 1 cut(s) | 253 |
| MseI | TTAA | 6 cut(s) | 216, 227, 459, 888, 933, 1344 |
| MspI | CCGG | 4 cut(s) | 433, 628, 1251, 1262 |
| MspR9I | CCNGG | 6 cut(s) | 197, 465, 628, 629, 1251, 1262 |
| MunI | CAATTG | 2 cut(s) | 134, 942 |
| Mva1269I | GAATGC | 1 cut(s) | 970 |
| MvaI | CCWGG | 2 cut(s) | 197, 465 |
| MvnI | CGCG | 4 cut(s) | 390, 562, 998, 1420 |
| NciI | CCSGG | 4 cut(s) | 628, 629, 1251, 1262 |
| NcoI | CCATGG | 2 cut(s) | 147, 320 |
| NdeII | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| NlaIV | GGNNCC | 4 cut(s) | 333, 728, 804, 1401 |
| NmuCI | GTSAC | 1 cut(s) | 1274 |
| NspI | RCATGY | 4 cut(s) | 385, 411, 491, 1322 |
| PaeI | GCATGC | 1 cut(s) | 491 |
| PagI | TCATGA | 1 cut(s) | 763 |
| PciI | ACATGT | 1 cut(s) | 407 |
| PciSI | GCTCTTC | 1 cut(s) | 1294 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 803 |
| PctI | GAATGC | 1 cut(s) | 970 |
| PdmI | GAANNNNTTC | 1 cut(s) | 253 |
| PfeI | GAWTC | 1 cut(s) | 913 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1188 |
| PkrI | GCNGC | 8 cut(s) | 58, 141, 243, 294, 309, 746, 1036, 1075 |
| PleI | GAGTC | 1 cut(s) | 983 |
| PpsI | GAGTC | 1 cut(s) | 983 |
| Ppu21I | YACGTR | 1 cut(s) | 126 |
| PscI | ACATGT | 1 cut(s) | 407 |
| Psp6I | CCWGG | 2 cut(s) | 195, 463 |
| PspGI | CCWGG | 2 cut(s) | 195, 463 |
| PspN4I | GGNNCC | 4 cut(s) | 333, 728, 804, 1401 |
| PspOMI | GGGCCC | 1 cut(s) | 1399 |
| PspPI | GGNCC | 3 cut(s) | 609, 1399, 1400 |
| PsuI | RGATCY | 1 cut(s) | 802 |
| RsaI | GTAC | 5 cut(s) | 194, 412, 574, 1225, 1323 |
| RsaNI | GTAC | 5 cut(s) | 193, 411, 573, 1224, 1322 |
| SalI | GTCGAC | 1 cut(s) | 780 |
| SapI | GCTCTTC | 1 cut(s) | 1294 |
| SaqAI | TTAA | 6 cut(s) | 216, 227, 459, 888, 933, 1344 |
| SatI | GCNGC | 8 cut(s) | 57, 140, 242, 293, 308, 745, 1035, 1074 |
| Sau3AI | GATC | 7 cut(s) | 199, 277, 589, 802, 927, 1051, 1378 |
| Sau96I | GGNCC | 3 cut(s) | 609, 1399, 1400 |
| SchI | GAGTC | 1 cut(s) | 983 |
| ScrFI | CCNGG | 6 cut(s) | 197, 465, 628, 629, 1251, 1262 |
| SduI | GDGCHC | 3 cut(s) | 1289, 1403, 1498 |
| SfaNI | GCATC | 5 cut(s) | 28, 39, 544, 553, 842 |
| SfcI | CTRYAG | 1 cut(s) | 879 |
| SmaI | CCCGGG | 1 cut(s) | 629 |
| SmlI | CTYRAG | 2 cut(s) | 991, 1280 |
| SmoI | CTYRAG | 2 cut(s) | 991, 1280 |
| SpeI | ACTAGT | 1 cut(s) | 1220 |
| SphI | GCATGC | 1 cut(s) | 491 |
| SsiI | CCGC | 4 cut(s) | 140, 399, 998, 1034 |
| SspMI | CTAG | 3 cut(s) | 635, 1140, 1221 |
| StyD4I | CCNGG | 6 cut(s) | 195, 463, 626, 627, 1249, 1260 |
| StyI | CCWWGG | 4 cut(s) | 17, 147, 320, 539 |
| TaaI | ACNGT | 1 cut(s) | 779 |
| TaiI | ACGT | 3 cut(s) | 26, 128, 170 |
| TaqI | TCGA | 5 cut(s) | 107, 592, 781, 1194, 1453 |
| TatI | WGTACW | 1 cut(s) | 1321 |
| TauI | GCSGC | 2 cut(s) | 142, 1037 |
| TfiI | GAWTC | 1 cut(s) | 913 |
| Tru1I | TTAA | 6 cut(s) | 216, 227, 459, 888, 933, 1344 |
| Tru9I | TTAA | 6 cut(s) | 216, 227, 459, 888, 933, 1344 |
| TscAI | CASTG | 4 cut(s) | 449, 658, 782, 915 |
| TseFI | GTSAC | 1 cut(s) | 1274 |
| TseI | GCWGC | 6 cut(s) | 56, 241, 292, 307, 744, 1073 |
| Tsp45I | GTSAC | 1 cut(s) | 1274 |
| TspDTI | ATGAA | 5 cut(s) | 711, 735, 1056, 1338, 1360 |
| TspGWI | ACGGA | 4 cut(s) | 812, 935, 1167, 1444 |
| TspMI | CCCGGG | 1 cut(s) | 627 |
| TspRI | CASTG | 4 cut(s) | 449, 658, 782, 915 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1188 |
| XapI | RAATTY | 4 cut(s) | 103, 426, 698, 1449 |
| XbaI | TCTAGA | 1 cut(s) | 1139 |
| XceI | RCATGY | 4 cut(s) | 385, 411, 491, 1322 |
| XmaI | CCCGGG | 1 cut(s) | 627 |
| XmiI | GTMKAC | 1 cut(s) | 781 |
| XmnI | GAANNNNTTC | 1 cut(s) | 253 |
| XspI | CTAG | 3 cut(s) | 635, 1140, 1221 |