Rorug01G0249100

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000044
Physical Location & Seq
Forward (+)
35732201 .. 35734987
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0249100.1

Sequence Viewer

Length: 1956 bp
ATGAATCCCCACAAAACAGAGGAAGACCTCTTCCACCATCACCACCACCATTTCCACCACCACCTCCACAACCATGATCTGGACCCACCGCATCCTCCTCCGATCGTCCAATCCGAGCCGTCCATTCCCCTCCCGCAAAACGACGTCGTCGAGCCGTTGTTCACTCCTCTCCCCGAAATCGACCTCTTCCGCTCCTCCTCCACCTCCTCCTCCCCGGCCCACTCCTCCTCCGAAAACGAAAACGACGACGTTTCGAACCCTAACCCTAGCTCGGCCCCGAGCAACAGCGCCGCCGCCGCGATGAAATCGACGCCGCTCTACATCAGCCCGGAGCCGCACATCTCGTCGCAGTTCTACACCTTCAACCTGGAGTCCCACTCCCTCATGATCCGCTGCCTCATGGAGGCCCGCCTCGCCACCCCCGCCGAGATCCGCGACGCCACCCCCGCCGACGTGCTCAAGTCGTGGCGCGCCGTCTGGAAGGACCGGAACGAGGACACCGCTTACGTCACGGCCTGGAAGCGCATCCAGGACAAGCTCTCGGCGCACCTCGACCAAAACGGTAACGAATTTCTGTGCTTCAAGAACAATACTCAGCAATTCGTTTCGCACATTAATCAGTGGCAGGATATTGTTATGGCTTTTCATAGTGATACCGATTTGAAACACTTGGGGCTTAAGGAAACCATTGATAGGATTAAGCAGGTCTGGACTGTAGGTGCTAAGTTTTACGGCATTCCCGAGAGTTACATTAGAGTCTGTGTTGCGGCCTGTCCTGTCTGTTCCTCTGCCTCCTCGGGGCTTTCGGCCAGGAATAAGAGGCGGCGGTTTGAGTATACCGAGTCGTTTGATGTGCCGGCGAAGGAGGTGCCGCATAAGCTTCAGCAGCTCGCTGCAAAGCATAAGGTGGTGCTTTGCATTAGGCAGAAGTATATTAGGTATAAGCCGTTTATGGCTGAGGTTAAGGACTATGCTTGCCACAGGGCGGGTGAGCCGGCGGCGAAGAAGTCTAAGATACTGAAGAGGGAGCCCTATGCCTCGAAGCGGTGTGGGTGTGGGTTTCGGATTAGGGCCATAGTTCCTATTACGAATTATAATGAGAAGGACAAGACGTTTGTGTATCAGGAAGAGGGGATGGCTGTGTTTAAGTTGTATGCTGTGCATTCTGGGCATGAGCCGGGACCGCTGGATGGGAATGCGAGGATTATGCATCGGGTTGTGGGGCACAAAGGTGGGTTTTTGATGGATCAAGATACTGTGTATGGGGTAAGTGATGAATTTGATAACGAGGGGTTTGGATTGTTGGGGAAGGATGAAGGGGATTTGCACCATTCAGTTTTGCAGCAGGTGAAGGAGTTGAGAGCTGAAGTTGGCTGTTTAGAAGAGAAAATTGCAAAAATCCCACAAGAGCTTTTGGGATCAGTGTCTCGAGAATTGTTTGATATTGTGAATAAAGTTAGACATATAGGTGAGGGAAGTTCGAAGACGATGGGATTGCTCCAGGACAAGCCAGGATCAGATGATATTTTGGTCGGGGAGAATGATTTGGCACATTGGACTGATAACCACCATGATCGTCTATATGGAGATGGACAGGACGCTGATTTGATTGAGGATGATGAAGATAGTTTTGGGCGAACTCTTGGGGATGTTGTCCCTTGGGAACAGATGAGGACAGAGTGTAGGAGTCAGAAGGATCTGATCAGTGAAGCTTGTAAGCCTGAGAAGTGGTTGAAATGCAGTGACTTCGATGAAAAGAGCATCCTTGATTGCCAGGATACTAAACTAACCAAGCCCTTATCACAAGACGAGAGTATAGTGACAGATGTAGGTCTTATTGGTTTACAGGTTGATAGTTTCTACCAAGGGACTTCTAAATGGTATGATTCTCCTTGTGGGTTGGACCCAGGTGCGGATTGTGGGGATAGTGCATTCAGGCATGGGGGGATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

651

Amino Acids

73.25

Weight (kDa)

5.77

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07760 AT5G28823
fragaria_vesca FvH4_1g13572 FvH4_2g03281 FvH4_2g04691 FvH4_2g08332 FvH4_2g08333 FvH4_2g15313 FvH4_2g26101 FvH4_2g29541 FvH4_3g06911 FvH4_3g34351 FvH4_3g43733 FvH4_4g02432 FvH4_4g05601 FvH4_4g08213 FvH4_4g09961 FvH4_4g10131 FvH4_4g20001 FvH4_4g20002 FvH4_5g01051 FvH4_5g01401 FvH4_5g27943 FvH4_6g09220 FvH4_6g17383 FvH4_6g35491 FvH4_6g46421 FvH4_7g04151 FvH4_7g13962 FvH4_7g18611 FvH4_7g19491 FvH4_7g19511 FvH4_7g26562
malus_domestica MD06G1221600.v1.1
pyrus_communis pycom10g21150
rosa_chinensis RchiOBHm_Chr1g0337571 RchiOBHm_Chr2g0168771 RchiOBHm_Chr3g0453631 RchiOBHm_Chr6g0278581
rosa_laevigata RLG00000001899 RLG00000001925 RLG00000002026 RLG00000003410 RLG00000003767 RLG00000004304 RLG00000004473 RLG00000004677 RLG00000006856 RLG00000007994 RLG00000009888 RLG00000011627 RLG00000012174 RLG00000012217 RLG00000013118 RLG00000013736 RLG00000014194 RLG00000014251 RLG00000015011 RLG00000015057 RLG00000015235 RLG00000017416 RLG00000018052 RLG00000018537 RLG00000019968 RLG00000020096 RLG00000020263 RLG00000022606 RLG00000023119 RLG00000028111 RLG00000029686 RLG00000030126 RLG00000031124 RLG00000031236 RLG00000032766 RLG00000033171 RLG00000033548 RLG00000033987 RLG00000034062 RLG00000035115 RLG00000035863
rosa_multiflora Rmu_co8036712.1_g000001 Rmu_co8080248.1_g000001 Rmu_co8208334.1_g000001 Rmu_co8253451.1_g000001 Rmu_co8393241.1_g000001 Rmu_co8522225.1_g000005 Rmu_sc0000060.1_g000030 Rmu_sc0000060.1_g000031 Rmu_sc0000060.1_g000032 Rmu_sc0000090.1_g000008 Rmu_sc0000149.1_g000051 Rmu_sc0000149.1_g000058 Rmu_sc0000309.1_g000050 Rmu_sc0000370.1_g000022 Rmu_sc0000370.1_g000023 Rmu_sc0000370.1_g000024 Rmu_sc0000418.1_g000018 Rmu_sc0000427.1_g000014 Rmu_sc0000427.1_g000015 Rmu_sc0000434.1_g000008 Rmu_sc0000487.1_g000038 Rmu_sc0000487.1_g000039 Rmu_sc0000569.1_g000029 Rmu_sc0000580.1_g000097 Rmu_sc0000595.1_g000068 Rmu_sc0000607.1_g000027 Rmu_sc0000738.1_g000004 Rmu_sc0000758.1_g000001 Rmu_sc0000758.1_g000002 Rmu_sc0000870.1_g000019 Rmu_sc0000924.1_g000040 Rmu_sc0000924.1_g000041 Rmu_sc0000999.1_g000037 Rmu_sc0001001.1_g000001 Rmu_sc0001010.1_g000080 Rmu_sc0001075.1_g000041 Rmu_sc0001097.1_g000016 Rmu_sc0001173.1_g000020 Rmu_sc0001258.1_g000010 Rmu_sc0001425.1_g000031 Rmu_sc0001493.1_g000020 Rmu_sc0001493.1_g000021 Rmu_sc0001644.1_g000024 Rmu_sc0001648.1_g000039 Rmu_sc0001662.1_g000002 Rmu_sc0001849.1_g000046 Rmu_sc0001851.1_g000038 Rmu_sc0001861.1_g000006 Rmu_sc0001899.1_g000008 Rmu_sc0002231.1_g000023 Rmu_sc0002393.1_g000012 Rmu_sc0002515.1_g000010 Rmu_sc0002551.1_g000013 Rmu_sc0002680.1_g000029 Rmu_sc0002700.1_g000030 Rmu_sc0002700.1_g000031 Rmu_sc0002709.1_g000035 Rmu_sc0002779.1_g000017 Rmu_sc0003267.1_g000040 Rmu_sc0003306.1_g000009 Rmu_sc0003338.1_g000014 Rmu_sc0003522.1_g000038 Rmu_sc0003522.1_g000039 Rmu_sc0003653.1_g000031 Rmu_sc0003901.1_g000003 Rmu_sc0003920.1_g000009 Rmu_sc0004014.1_g000009 Rmu_sc0004016.1_g000013 Rmu_sc0004031.1_g000002 Rmu_sc0004106.1_g000001 Rmu_sc0004432.1_g000004 Rmu_sc0004481.1_g000020 Rmu_sc0004874.1_g000005 Rmu_sc0004928.1_g000005 Rmu_sc0005065.1_g000053 Rmu_sc0005091.1_g000005 Rmu_sc0005217.1_g000015 Rmu_sc0005242.1_g000002 Rmu_sc0005300.1_g000011 Rmu_sc0005400.1_g000004 Rmu_sc0005486.1_g000007 Rmu_sc0005527.1_g000015 Rmu_sc0006059.1_g000021 Rmu_sc0006087.1_g000004 Rmu_sc0006184.1_g000006 Rmu_sc0006184.1_g000007 Rmu_sc0006578.1_g000006 Rmu_sc0006730.1_g000010 Rmu_sc0006736.1_g000030 Rmu_sc0006736.1_g000033 Rmu_sc0007333.1_g000018 Rmu_sc0007856.1_g000010 Rmu_sc0008231.1_g000003 Rmu_sc0009862.1_g000012 Rmu_sc0010547.1_g000001 Rmu_sc0010547.1_g000003 Rmu_sc0010632.1_g000012 Rmu_sc0010747.1_g000008 Rmu_sc0011075.1_g000009 Rmu_sc0011565.1_g000004 Rmu_sc0011641.1_g000003 Rmu_sc0013102.1_g000013 Rmu_sc0013160.1_g000006 Rmu_sc0014846.1_g000004 Rmu_sc0017037.1_g000003 Rmu_sc0018203.1_g000001 Rmu_sc0018204.1_g000001 Rmu_sc0018348.1_g000001 Rmu_sc0019354.1_g000002 Rmu_sc0021630.1_g000002 Rmu_sc0022987.1_g000002 Rmu_sc0024469.1_g000005 Rmu_sc0033519.1_g000001 Rmu_sc0033519.1_g000002 Rmu_sc0033519.1_g000003 Rmu_sc0042775.1_g000001 Rmu_ssc0000110.1_g000003 Rmu_ssc0000110.1_g000004 Rmu_ssc0000126.1_g000068 Rmu_ssc0000200.1_g000007 Rmu_ssc0000295.1_g000003 Rmu_ssc0000295.1_g000004 Rmu_ssc0000299.1_g000010 Rmu_ssc0000308.1_g000024 Rmu_ssc0000339.1_g000006 Rmu_ssc0000385.1_g000012 Rmu_ssc0000409.1_g000004 Rmu_ssc0000421.1_g000004
rosa_roxburghii Rroxscaffold_156G00440360 Rroxscaffold_166G00440800 Rroxscaffold_1G00039040 Rroxscaffold_1G00039050 Rroxscaffold_1G00073950 Rroxscaffold_2G00091710 Rroxscaffold_2G00120050 Rroxscaffold_2G00122590 Rroxscaffold_3G00219700 Rroxscaffold_3G00264140 Rroxscaffold_4G00290600 Rroxscaffold_4G00323610 Rroxscaffold_4G00328460 Rroxscaffold_5G00343440 Rroxscaffold_5G00343570 Rroxscaffold_5G00378540 Rroxscaffold_5G00385090 Rroxscaffold_7G00169130 Rroxscaffold_7G00216420
rosa_rugosa Rorug01G0065800 Rorug01G0073500 Rorug01G0091700 Rorug01G0096500 Rorug01G0204700.1 Rorug01G0249100 Rorug01G0333800 Rorug01G0333900 Rorug01G0339500 Rorug01G0431300 Rorug02G0085400 Rorug02G0117500 Rorug02G0130900 Rorug02G0178800 Rorug02G0226700 Rorug02G0336300 Rorug02G0338700 Rorug02G0351400 Rorug02G0459900 Rorug03G0187000 Rorug03G0223700 Rorug03G0291300 Rorug03G0291300 Rorug03G0311100 Rorug03G0316900 Rorug03G0346000 Rorug04G0014500 Rorug04G0014600 Rorug04G0124100 Rorug04G0136500 Rorug04G0302000 Rorug04G0338100 Rorug04G0338200 Rorug04G0413100 Rorug05G0182200 Rorug05G0226500 Rorug05G0273300 Rorug05G0292200 Rorug05G0292200 Rorug05G0392500 Rorug05G0394500 Rorug05G0394600 Rorug05G0505600.1 Rorug06G0351400 Rorug06G0393400 Rorug06G0393600 Rorug06G0470000 Rorug07G0063100 Rorug07G0086800 Rorug07G0109200 Rorug07G0236100
rosa_samantha Rh1AG010800 Rh1AG011100 Rh1AG064900 Rh1AG088000 Rh1AG207100 Rh1AG262000 Rh1AG300800 Rh1BG043000 Rh1BG144600 Rh1BG180700 Rh1BG180800 Rh1BG328800 Rh1BG399400 Rh1CG041400 Rh1CG047300 Rh1CG379700 Rh1DG036500 Rh1DG083000 Rh1DG210700 Rh1DG210800 Rh1DG231100 Rh2AG007900 Rh2AG055500 Rh2AG339100 Rh2AG368100 Rh2BG243300 Rh2BG258900 Rh2BG292000 Rh2BG333500 Rh2BG387300 Rh2BG612800 Rh2BG619100 Rh2CG026500 Rh2CG163600 Rh2CG535700 Rh2DG186400 Rh2DG235000 Rh2DG314500 Rh2DG388500 Rh2DG391400 Rh2DG496900 Rh2DG526700 Rh2DG570700 Rh3AG330700 Rh3BG266900 Rh3CG236000 Rh3CG340400 Rh3DG090000 Rh3DG278400 Rh3DG297600 Rh3DG362600 Rh4AG001300 Rh4AG023300 Rh4AG097100 Rh4AG427000 Rh4BG029400 Rh4BG032400 Rh4BG086000 Rh4BG249200 Rh4CG001900 Rh4CG040600 Rh4CG093000 Rh4CG106300 Rh4CG113200 Rh4CG113900 Rh4DG002300 Rh4DG144400 Rh4DG234200 Rh5AG043700 Rh5AG084900 Rh5AG161500 Rh5AG409000 Rh5AG457200 Rh5AG457900 Rh5AG473700 Rh5AG520600 Rh5BG042100 Rh5BG124100 Rh5BG323500 Rh5BG417200 Rh5BG443700 Rh5BG451500 Rh5BG464900 Rh5BG470400 Rh5BG522500 Rh5CG068800 Rh5CG178300 Rh5CG231000 Rh5CG258600 Rh5CG394500 Rh5CG473700 Rh5DG164700 Rh5DG235400 Rh5DG261300 Rh5DG304900 Rh5DG334500 Rh5DG424300 Rh5DG458400 Rh5DG478600 Rh5DG552300 Rh5DG552700 Rh6AG037000 Rh6AG042100 Rh6AG255500 Rh6AG283400 Rh6AG291800 Rh6BG049600 Rh6BG080700 Rh6BG114600 Rh6BG128900 Rh6BG352500 Rh6CG052400 Rh6CG112900 Rh6CG113000 Rh6CG131300 Rh6CG157700 Rh6CG243200 Rh6CG254100 Rh6CG275700 Rh6CG299700 Rh6CG309100 Rh6CG317600 Rh6CG342600 Rh6CG471900 Rh6DG038200 Rh6DG102300 Rh6DG114400 Rh6DG225900 Rh6DG292900 Rh6DG320700 Rh6DG512800 Rh7AG217700 Rh7AG313900 Rh7AG446000 Rh7AG472600 Rh7BG223100 Rh7BG349900 Rh7BG352000 Rh7CG160200 Rh7CG233900 Rh7CG242700 Rh7CG273900 Rh7CG306400 Rh7CG322200 Rh7DG053800 Rh7DG077800 Rh7DG227700 Rh7DG404800
rosa_wichuraiana Rw0G003990 Rw2G022110 Rw2G024890 Rw6G005700 Rw7G008770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
AarI CACCTGC 1 cut(s) 1336
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 2 cut(s) 694, 1336
AccB1I GGYRCC 1 cut(s) 868
AccB7I CCANNNNNTGG 1 cut(s) 79
AccBSI CCGCTC 2 cut(s) 192, 316
AccI GTMKAC 1 cut(s) 836
AccII CGCG 3 cut(s) 299, 435, 471
AclWI GGATC 6 cut(s) 382, 424, 1254, 1426, 1522, 1704
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 2 cut(s) 569, 1277
AcuI CTGAAG 3 cut(s) 866, 1040, 1386
AcyI GRCGYC 3 cut(s) 144, 311, 438
AflII CTTAAG 1 cut(s) 677
AgsI TTSAA 4 cut(s) 364, 583, 664, 1735
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 8 cut(s) 366, 515, 528, 809, 1500, 1510, 1773, 1906
AjuI GAANNNNNNNTTGG 2 cut(s) 1614, 1646
AleI CACNNNNGTG 1 cut(s) 1230
AluBI AGCT 7 cut(s) 270, 538, 880, 889, 1364, 1411, 1712
AluI AGCT 7 cut(s) 270, 538, 880, 889, 1364, 1411, 1712
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 1 cut(s) 1431
AlwI GGATC 6 cut(s) 382, 424, 1254, 1426, 1522, 1704
Ama87I CYCGRG 4 cut(s) 277, 740, 796, 1428
AoxI GGCC 7 cut(s) 216, 273, 405, 513, 768, 807, 1071
ApeKI GCWGC 4 cut(s) 393, 886, 893, 1342
ApoI RAATTY 2 cut(s) 569, 1277
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AscI GGCGCGCC 1 cut(s) 469
AseI ATTAAT 1 cut(s) 615
AspLEI GCGC 5 cut(s) 290, 471, 473, 525, 547
AspS9I GGNCC 8 cut(s) 82, 217, 274, 406, 484, 1071, 1181, 1903
AsuC2I CCSGG 3 cut(s) 215, 329, 1179
AsuHPI GGTGA 4 cut(s) 32, 1001, 1360, 1481
AsuII TTCGAA 2 cut(s) 254, 1481
AvaI CYCGRG 4 cut(s) 277, 740, 796, 1428
AvaII GGWCC 4 cut(s) 82, 484, 1181, 1903
BaeGI GKGCMC 1 cut(s) 1227
BanI GGYRCC 1 cut(s) 868
BanII GRGCYC 1 cut(s) 1032
BbsI GAAGAC 2 cut(s) 30, 1490
Bbv12I GWGCWC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 957
BbvI GCAGC 4 cut(s) 380, 880, 898, 1354
BccI CCATC 6 cut(s) 45, 1129, 1184, 1237, 1483, 1583
BceAI ACGGC 6 cut(s) 103, 139, 458, 528, 748, 931
BcgI CGANNNNNNTGC 8 cut(s) 850, 884, 1189, 1223, 1477, 1511, 1729, 1763
BciT130I CCWGG 8 cut(s) 368, 517, 530, 811, 1502, 1512, 1775, 1908
BciVI GTATCC 1 cut(s) 1771
BclI TGATCA 1 cut(s) 1701
BcnI CCSGG 3 cut(s) 215, 329, 1179
BcoDI GTCTC 1 cut(s) 1431
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 714
BfoI RGCGCY 1 cut(s) 291
BfrI CTTAAG 1 cut(s) 677
BfuAI ACCTGC 2 cut(s) 694, 1336
BfuI GTATCC 1 cut(s) 1771
Bme18I GGWCC 4 cut(s) 82, 484, 1181, 1903
BmeT110I CYCGRG 4 cut(s) 277, 740, 796, 1428
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 8 cut(s) 82, 217, 274, 406, 484, 1071, 1181, 1903
BmiI GGNNCC 7 cut(s) 84, 276, 333, 870, 1029, 1182, 1905
BmsI GCATC 4 cut(s) 100, 534, 1219, 1770
BpiI GAAGAC 2 cut(s) 30, 1490
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpmI CTGGAG 2 cut(s) 389, 1484
Bpu10I CCTNAGC 1 cut(s) 957
Bpu14I TTCGAA 2 cut(s) 254, 1481
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 3 cut(s) 215, 329, 1179
BsaHI GRCGYC 3 cut(s) 144, 311, 438
BsaJI CCNNGG 5 cut(s) 213, 795, 1658, 1864, 1906
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse118I RCCGGY 2 cut(s) 856, 994
BseBI CCWGG 8 cut(s) 368, 517, 530, 811, 1502, 1512, 1775, 1908
BseDI CCNNGG 5 cut(s) 213, 795, 1658, 1864, 1906
BseGI GGATG 8 cut(s) 91, 525, 1140, 1195, 1318, 1621, 1654, 1761
BseMII CTCAG 3 cut(s) 608, 948, 1713
BsePI GCGCGC 1 cut(s) 469
BseRI GAGGAG 9 cut(s) 87, 156, 184, 187, 196, 199, 214, 217, 784
BseSI GKGCMC 1 cut(s) 1227
BseXI GCAGC 4 cut(s) 380, 880, 898, 1354
Bsh1236I CGCG 3 cut(s) 299, 435, 471
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 7 cut(s) 218, 275, 407, 515, 770, 809, 1073
BshNI GGYRCC 1 cut(s) 868
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 4 cut(s) 277, 740, 796, 1428
BsiSI CCGG 6 cut(s) 215, 329, 487, 857, 995, 1178
BslFI GGGAC 4 cut(s) 358, 1194, 1640, 1882
BsmAI GTCTC 1 cut(s) 1431
BsmFI GGGAC 4 cut(s) 358, 1194, 1640, 1882
BsmI GAATGC 4 cut(s) 735, 1162, 1201, 1931
BsnI GGCC 7 cut(s) 218, 275, 407, 515, 770, 809, 1073
BsoBI CYCGRG 4 cut(s) 277, 740, 796, 1428
Bsp119I TTCGAA 2 cut(s) 254, 1481
Bsp1286I GDGCHC 3 cut(s) 459, 1032, 1227
BspANI GGCC 7 cut(s) 218, 275, 407, 515, 770, 809, 1073
BspCNI CTCAG 3 cut(s) 607, 949, 1714
BspFNI CGCG 3 cut(s) 299, 435, 471
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 7 cut(s) 84, 276, 333, 870, 1029, 1182, 1905
BspMI ACCTGC 2 cut(s) 694, 1336
BspPI GGATC 6 cut(s) 382, 424, 1254, 1426, 1522, 1704
BspT104I TTCGAA 2 cut(s) 254, 1481
BspT107I GGYRCC 1 cut(s) 868
BspTI CTTAAG 1 cut(s) 677
BsrBI CCGCTC 2 cut(s) 192, 316
BsrFI RCCGGY 2 cut(s) 856, 994
BssAI RCCGGY 2 cut(s) 856, 994
BssECI CCNNGG 5 cut(s) 213, 795, 1658, 1864, 1906
BssHII GCGCGC 1 cut(s) 469
BssNAI GTATAC 1 cut(s) 837
BssNI GRCGYC 3 cut(s) 144, 311, 438
BssT1I CCWWGG 2 cut(s) 1658, 1864
Bst1107I GTATAC 1 cut(s) 837
Bst2UI CCWGG 8 cut(s) 368, 517, 530, 811, 1502, 1512, 1775, 1908
Bst4CI ACNGT 3 cut(s) 563, 715, 1258
Bst6I CTCTTC 5 cut(s) 35, 191, 1016, 1122, 1377
BstACI GRCGYC 3 cut(s) 144, 311, 438
BstAFI CTTAAG 1 cut(s) 677
BstBI TTCGAA 2 cut(s) 254, 1481
BstC8I GCNNGC 6 cut(s) 409, 471, 858, 891, 976, 996
BstDEI CTNAG 5 cut(s) 594, 723, 957, 1011, 1722
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 8 cut(s) 91, 525, 1140, 1195, 1318, 1621, 1654, 1761
BstFNI CGCG 3 cut(s) 299, 435, 471
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 5 cut(s) 290, 471, 473, 525, 547
BstMAI GTCTC 1 cut(s) 1431
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 9 cut(s) 296, 413, 422, 446, 544, 877, 886, 1168, 1183
BstNI CCWGG 8 cut(s) 368, 517, 530, 811, 1502, 1512, 1775, 1908
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 1227
BstUI CGCG 3 cut(s) 299, 435, 471
BstV1I GCAGC 4 cut(s) 380, 880, 898, 1354
BstV2I GAAGAC 2 cut(s) 30, 1490
BstX2I RGATCY 2 cut(s) 429, 1696
BstYI RGATCY 2 cut(s) 429, 1696
BstZ17I GTATAC 1 cut(s) 837
BsuI GTATCC 1 cut(s) 1771
BsuRI GGCC 7 cut(s) 218, 275, 407, 515, 770, 809, 1073
BtgZI GCGATG 1 cut(s) 314
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 8 cut(s) 91, 525, 1140, 1195, 1318, 1621, 1654, 1761
BtsI GCAGTG 1 cut(s) 1747
BtsIMutI CAGTG 4 cut(s) 626, 1428, 1711, 1747
BveI ACCTGC 2 cut(s) 694, 1336
Cac8I GCNNGC 6 cut(s) 409, 471, 858, 891, 976, 996
CciI TCATGA 1 cut(s) 384
CfoI GCGC 5 cut(s) 290, 471, 473, 525, 547
Cfr10I RCCGGY 2 cut(s) 856, 994
Cfr13I GGNCC 8 cut(s) 82, 217, 274, 406, 484, 1071, 1181, 1903
CseI GACGC 3 cut(s) 319, 446, 1607
CviAII CATG 6 cut(s) 74, 385, 400, 1172, 1571, 1940
DdeI CTNAG 5 cut(s) 594, 723, 957, 1011, 1722
EaeI YGGCCR 1 cut(s) 807
Eam1104I CTCTTC 5 cut(s) 35, 191, 1016, 1122, 1377
EarI CTCTTC 5 cut(s) 35, 191, 1016, 1122, 1377
Eco130I CCWWGG 2 cut(s) 1658, 1864
Eco24I GRGCYC 1 cut(s) 1032
Eco47I GGWCC 4 cut(s) 82, 484, 1181, 1903
Eco57I CTGAAG 3 cut(s) 866, 1040, 1386
Eco88I CYCGRG 4 cut(s) 277, 740, 796, 1428
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoRII CCWGG 8 cut(s) 366, 515, 528, 809, 1500, 1510, 1773, 1906
EcoT14I CCWWGG 2 cut(s) 1658, 1864
EcoT22I ATGCAT 1 cut(s) 1212
EcoT38I GRGCYC 1 cut(s) 1032
ErhI CCWWGG 2 cut(s) 1658, 1864
FaeI CATG 6 cut(s) 77, 388, 403, 1175, 1574, 1943
FaqI GGGAC 4 cut(s) 358, 1194, 1640, 1882
FatI CATG 6 cut(s) 73, 384, 399, 1171, 1570, 1939
FauI CCCGC 5 cut(s) 141, 416, 430, 454, 979
FbaI TGATCA 1 cut(s) 1701
FblI GTMKAC 1 cut(s) 836
FokI GGATG 8 cut(s) 78, 512, 1147, 1202, 1325, 1628, 1661, 1748
FriOI GRGCYC 1 cut(s) 1032
FspBI CTAG 1 cut(s) 267
GlaI GCGC 5 cut(s) 289, 470, 472, 524, 546
GsuI CTGGAG 2 cut(s) 389, 1484
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 7 cut(s) 218, 275, 407, 515, 770, 809, 1073
HapII CCGG 6 cut(s) 215, 329, 487, 857, 995, 1178
HgaI GACGC 3 cut(s) 319, 446, 1607
HhaI GCGC 5 cut(s) 290, 471, 473, 525, 547
Hin1I GRCGYC 3 cut(s) 144, 311, 438
Hin1II CATG 6 cut(s) 77, 388, 403, 1175, 1574, 1943
Hin6I GCGC 5 cut(s) 288, 469, 471, 523, 545
HinP1I GCGC 5 cut(s) 288, 469, 471, 523, 545
HindIII AAGCTT 2 cut(s) 878, 1710
HinfI GANTC 6 cut(s) 4, 371, 756, 842, 1687, 1886
HpaII CCGG 6 cut(s) 215, 329, 487, 857, 995, 1178
HphI GGTGA 4 cut(s) 32, 1001, 1360, 1481
Hpy166II GTNNAC 3 cut(s) 162, 837, 1844
Hpy188I TCNGA 7 cut(s) 102, 115, 232, 1065, 1519, 1692, 1701
Hpy8I GTNNAC 3 cut(s) 162, 837, 1844
Hpy99I CGWCG 9 cut(s) 146, 149, 152, 248, 251, 313, 349, 440, 455
HpyAV CCTTC 8 cut(s) 370, 475, 856, 1096, 1303, 1310, 1345, 1687
HpyCH4III ACNGT 3 cut(s) 563, 715, 1258
HpyCH4IV ACGT 5 cut(s) 144, 249, 453, 507, 1112
HpyCH4V TGCA 9 cut(s) 896, 918, 1162, 1210, 1327, 1342, 1394, 1740, 1931
HpyF10VI GCNNNNNNNGC 9 cut(s) 296, 413, 422, 446, 544, 877, 886, 1168, 1183
HpyF3I CTNAG 5 cut(s) 594, 723, 957, 1011, 1722
HpySE526I ACGT 5 cut(s) 144, 249, 453, 507, 1112
Hsp92I GRCGYC 3 cut(s) 144, 311, 438
Hsp92II CATG 6 cut(s) 77, 388, 403, 1175, 1574, 1943
HspAI GCGC 5 cut(s) 288, 469, 471, 523, 545
KroI GCCGGC 2 cut(s) 856, 994
KroNI GCCGGC 2 cut(s) 858, 996
Ksp22I TGATCA 1 cut(s) 1701
LmnI GCTCC 4 cut(s) 197, 331, 1027, 1503
Lsp1109I GCAGC 4 cut(s) 380, 880, 898, 1354
LweI GCATC 4 cut(s) 100, 534, 1219, 1770
MaeI CTAG 1 cut(s) 267
MaeII ACGT 5 cut(s) 144, 249, 453, 507, 1112
MaeIII GTNAC 5 cut(s) 508, 563, 746, 1742, 1819
MbiI CCGCTC 2 cut(s) 192, 316
MboII GAAGA 9 cut(s) 22, 35, 178, 1015, 1033, 1139, 1394, 1495, 1634
MflI RGATCY 2 cut(s) 429, 1696
MhlI GDGCHC 3 cut(s) 459, 1032, 1227
MluCI AATT 6 cut(s) 569, 599, 1090, 1277, 1389, 1433
MlyI GAGTC 4 cut(s) 380, 765, 851, 1696
MmeI TCCRAC 1 cut(s) 1881
Mph1103I ATGCAT 1 cut(s) 1212
MroNI GCCGGC 2 cut(s) 856, 994
MseI TTAA 5 cut(s) 615, 678, 699, 963, 1146
MslI CAYNNNNRTG 3 cut(s) 72, 1230, 1467
MspA1I CMGCKG 2 cut(s) 393, 1186
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 6 cut(s) 215, 329, 487, 857, 995, 1178
Mva1269I GAATGC 4 cut(s) 735, 1162, 1201, 1931
MvaI CCWGG 8 cut(s) 368, 517, 530, 811, 1502, 1512, 1775, 1908
MvnI CGCG 3 cut(s) 299, 435, 471
MwoI GCNNNNNNNGC 9 cut(s) 296, 413, 422, 446, 544, 877, 886, 1168, 1183
NaeI GCCGGC 2 cut(s) 858, 996
NciI CCSGG 3 cut(s) 215, 329, 1179
NgoMIV GCCGGC 2 cut(s) 856, 994
NlaIII CATG 6 cut(s) 77, 388, 403, 1175, 1574, 1943
NlaIV GGNNCC 7 cut(s) 84, 276, 333, 870, 1029, 1182, 1905
NmeAIII GCCGAG 3 cut(s) 251, 451, 521
NmuCI GTSAC 3 cut(s) 508, 1742, 1819
NsiI ATGCAT 1 cut(s) 1212
NspV TTCGAA 2 cut(s) 254, 1481
OliI CACNNNNGTG 1 cut(s) 1230
PaeR7I CTCGAG 1 cut(s) 1428
PagI TCATGA 1 cut(s) 384
PalAI GGCGCGCC 1 cut(s) 469
PaqCI CACCTGC 1 cut(s) 1336
PauI GCGCGC 1 cut(s) 469
PcsI WCGNNNNNNNCGW 4 cut(s) 111, 147, 243, 738
PctI GAATGC 4 cut(s) 735, 1162, 1201, 1931
PdiI GCCGGC 2 cut(s) 858, 996
PfeI GAWTC 2 cut(s) 4, 1886
PflFI GACNNNGTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 79
PfoI TCCNGGA 2 cut(s) 528, 1500
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 4 cut(s) 379, 764, 850, 1695
PpsI GAGTC 4 cut(s) 379, 764, 850, 1695
PshBI ATTAAT 1 cut(s) 615
PsiI TTATAA 1 cut(s) 1095
Psp6I CCWGG 8 cut(s) 366, 515, 528, 809, 1500, 1510, 1773, 1906
PspGI CCWGG 8 cut(s) 366, 515, 528, 809, 1500, 1510, 1773, 1906
PspN4I GGNNCC 7 cut(s) 84, 276, 333, 870, 1029, 1182, 1905
PspPI GGNCC 8 cut(s) 82, 217, 274, 406, 484, 1071, 1181, 1903
PsuI RGATCY 2 cut(s) 429, 1696
PsyI GACNNNGTC 1 cut(s) 146
PteI GCGCGC 1 cut(s) 469
PvuI CGATCG 1 cut(s) 105
RseI CAYNNNNRTG 3 cut(s) 72, 1230, 1467
SaqAI TTAA 5 cut(s) 615, 678, 699, 963, 1146
Sau96I GGNCC 8 cut(s) 82, 217, 274, 406, 484, 1071, 1181, 1903
SchI GAGTC 4 cut(s) 380, 765, 851, 1696
SduI GDGCHC 3 cut(s) 459, 1032, 1227
SfaNI GCATC 4 cut(s) 100, 534, 1219, 1770
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 1428
SfuI TTCGAA 2 cut(s) 254, 1481
SgsI GGCGCGCC 1 cut(s) 469
SinI GGWCC 4 cut(s) 82, 484, 1181, 1903
SlaI CTCGAG 1 cut(s) 1428
SmiMI CAYNNNNRTG 3 cut(s) 72, 1230, 1467
SmlI CTYRAG 3 cut(s) 458, 677, 1428
SmoI CTYRAG 3 cut(s) 458, 677, 1428
Sse9I AATT 6 cut(s) 569, 599, 1090, 1277, 1389, 1433
SspMI CTAG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 1658, 1864
TaaI ACNGT 3 cut(s) 563, 715, 1258
TaiI ACGT 5 cut(s) 147, 252, 456, 510, 1115
TaqI TCGA 9 cut(s) 150, 180, 254, 308, 552, 1040, 1429, 1481, 1749
TasI AATT 6 cut(s) 569, 599, 1090, 1277, 1389, 1433
TauI GCSGC 9 cut(s) 293, 296, 299, 316, 337, 770, 826, 874, 1001
TfiI GAWTC 2 cut(s) 4, 1886
Tru1I TTAA 5 cut(s) 615, 678, 699, 963, 1146
Tru9I TTAA 5 cut(s) 615, 678, 699, 963, 1146
TscAI CASTG 4 cut(s) 626, 1428, 1711, 1747
TseFI GTSAC 3 cut(s) 508, 1742, 1819
TseI GCWGC 4 cut(s) 393, 886, 893, 1342
Tsp45I GTSAC 3 cut(s) 508, 1742, 1819
TspDTI ATGAA 7 cut(s) 17, 317, 635, 1290, 1329, 1635, 1767
TspRI CASTG 4 cut(s) 626, 1428, 1711, 1747
Tth111I GACNNNGTC 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 4 cut(s) 82, 484, 1181, 1903
VspI ATTAAT 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 2 cut(s) 569, 1277
XhoI CTCGAG 1 cut(s) 1428
XmiI GTMKAC 1 cut(s) 836
XspI CTAG 1 cut(s) 267
ZraI GACGTC 1 cut(s) 145
Zsp2I ATGCAT 1 cut(s) 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.