Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 15 |
| Acc36I | ACCTGC | 1 cut(s) | 105 |
| AccBSI | CCGCTC | 1 cut(s) | 278 |
| AccI | GTMKAC | 1 cut(s) | 9 |
| AccII | CGCG | 3 cut(s) | 497, 521, 936 |
| AciI | CCGC | 9 cut(s) | 5, 26, 65, 74, 276, 485, 497, 521, 934 |
| AclI | AACGTT | 1 cut(s) | 334 |
| AcsI | RAATTY | 2 cut(s) | 184, 791 |
| AcuI | CTGAAG | 1 cut(s) | 165 |
| AcyI | GRCGYC | 2 cut(s) | 12, 23 |
| AfaI | GTAC | 3 cut(s) | 581, 948, 1036 |
| AfeI | AGCGCT | 1 cut(s) | 660 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 64, 112, 288, 289, 867, 898 |
| AgsI | TTSAA | 1 cut(s) | 842 |
| AjiI | CACGTC | 1 cut(s) | 884 |
| AjnI | CCWGG | 2 cut(s) | 225, 628 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 141, 173 |
| AluBI | AGCT | 4 cut(s) | 605, 668, 675, 905 |
| AluI | AGCT | 4 cut(s) | 605, 668, 675, 905 |
| Alw26I | GTCTC | 2 cut(s) | 19, 441 |
| Aor51HI | AGCGCT | 1 cut(s) | 660 |
| AoxI | GGCC | 1 cut(s) | 256 |
| ApeKI | GCWGC | 7 cut(s) | 50, 304, 569, 661, 668, 695, 887 |
| ApoI | RAATTY | 2 cut(s) | 184, 791 |
| AspLEI | GCGC | 2 cut(s) | 661, 938 |
| AspS9I | GGNCC | 2 cut(s) | 229, 523 |
| AsuC2I | CCSGG | 2 cut(s) | 868, 985 |
| AsuHPI | GGTGA | 1 cut(s) | 800 |
| AvaII | GGWCC | 2 cut(s) | 229, 523 |
| BauI | CACGAG | 1 cut(s) | 637 |
| BbvI | GCAGC | 7 cut(s) | 37, 291, 556, 648, 655, 707, 874 |
| BccI | CCATC | 3 cut(s) | 520, 743, 883 |
| BciT130I | CCWGG | 2 cut(s) | 227, 630 |
| BcnI | CCSGG | 2 cut(s) | 868, 985 |
| BcoDI | GTCTC | 2 cut(s) | 19, 441 |
| BfaI | CTAG | 3 cut(s) | 260, 863, 906 |
| BfmI | CTRYAG | 1 cut(s) | 827 |
| BfoI | RGCGCY | 1 cut(s) | 662 |
| BfuAI | ACCTGC | 1 cut(s) | 105 |
| Bme1390I | CCNGG | 4 cut(s) | 227, 630, 868, 985 |
| Bme18I | GGWCC | 2 cut(s) | 229, 523 |
| BmgBI | CACGTC | 1 cut(s) | 884 |
| BmgT120I | GGNCC | 2 cut(s) | 229, 523 |
| BmiI | GGNNCC | 1 cut(s) | 591 |
| BmrFI | CCNGG | 4 cut(s) | 227, 630, 868, 985 |
| BmsI | GCATC | 5 cut(s) | 62, 164, 630, 747, 970 |
| BpmI | CTGGAG | 3 cut(s) | 331, 595, 783 |
| BpuMI | CCSGG | 2 cut(s) | 868, 985 |
| Bsa29I | ATCGAT | 1 cut(s) | 978 |
| BsaBI | GATNNNNATC | 1 cut(s) | 137 |
| BsaHI | GRCGYC | 2 cut(s) | 12, 23 |
| BsaJI | CCNNGG | 6 cut(s) | 160, 283, 318, 409, 628, 897 |
| BsaWI | WCCGGW | 2 cut(s) | 562, 688 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 64, 112, 288, 289, 867, 898 |
| Bse1I | ACTGG | 3 cut(s) | 348, 558, 800 |
| Bse8I | GATNNNNATC | 1 cut(s) | 137 |
| BseBI | CCWGG | 2 cut(s) | 227, 630 |
| BseCI | ATCGAT | 1 cut(s) | 978 |
| BseDI | CCNNGG | 6 cut(s) | 160, 283, 318, 409, 628, 897 |
| BseGI | GGATG | 3 cut(s) | 53, 376, 621 |
| BseJI | GATNNNNATC | 1 cut(s) | 137 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 64, 112, 288, 289, 867, 898 |
| BseMII | CTCAG | 3 cut(s) | 50, 105, 558 |
| BseNI | ACTGG | 3 cut(s) | 348, 558, 800 |
| BseRI | GAGGAG | 2 cut(s) | 131, 498 |
| BseXI | GCAGC | 7 cut(s) | 37, 291, 556, 648, 655, 707, 874 |
| Bsh1236I | CGCG | 3 cut(s) | 497, 521, 936 |
| Bsh1285I | CGRYCG | 1 cut(s) | 9 |
| BshFI | GGCC | 1 cut(s) | 258 |
| BshVI | ATCGAT | 1 cut(s) | 978 |
| BsiEI | CGRYCG | 1 cut(s) | 9 |
| BsiSI | CCGG | 5 cut(s) | 563, 587, 689, 868, 984 |
| BslFI | GGGAC | 1 cut(s) | 955 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 64, 112, 288, 289, 867, 898 |
| BsmAI | GTCTC | 2 cut(s) | 19, 441 |
| BsmBI | CGTCTC | 1 cut(s) | 19 |
| BsmFI | GGGAC | 1 cut(s) | 955 |
| BsnI | GGCC | 1 cut(s) | 258 |
| Bsp143I | GATC | 3 cut(s) | 401, 529, 990 |
| Bsp19I | CCATGG | 1 cut(s) | 318 |
| BspACI | CCGC | 9 cut(s) | 5, 26, 65, 74, 276, 485, 497, 521, 934 |
| BspANI | GGCC | 1 cut(s) | 258 |
| BspCNI | CTCAG | 3 cut(s) | 51, 104, 557 |
| BspDI | ATCGAT | 1 cut(s) | 978 |
| BspFNI | CGCG | 3 cut(s) | 497, 521, 936 |
| BspLI | GGNNCC | 1 cut(s) | 591 |
| BspMAI | CTGCAG | 1 cut(s) | 831 |
| BspMI | ACCTGC | 1 cut(s) | 105 |
| BsrBI | CCGCTC | 1 cut(s) | 278 |
| BsrI | ACTGG | 3 cut(s) | 348, 558, 800 |
| BssECI | CCNNGG | 6 cut(s) | 160, 283, 318, 409, 628, 897 |
| BssMI | GATC | 3 cut(s) | 401, 529, 990 |
| BssNI | GRCGYC | 2 cut(s) | 12, 23 |
| BssSI | CACGAG | 1 cut(s) | 637 |
| BssT1I | CCWWGG | 3 cut(s) | 283, 318, 897 |
| Bst2BI | CACGAG | 1 cut(s) | 637 |
| Bst2UI | CCWGG | 2 cut(s) | 227, 630 |
| Bst6I | CTCTTC | 1 cut(s) | 777 |
| BstACI | GRCGYC | 2 cut(s) | 12, 23 |
| BstC8I | GCNNGC | 4 cut(s) | 102, 489, 666, 673 |
| BstDEI | CTNAG | 4 cut(s) | 59, 91, 544, 655 |
| BstDSI | CCRYGG | 2 cut(s) | 318, 409 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 896 |
| BstF5I | GGATG | 3 cut(s) | 53, 376, 621 |
| BstFNI | CGCG | 3 cut(s) | 497, 521, 936 |
| BstH2I | RGCGCY | 1 cut(s) | 662 |
| BstHHI | GCGC | 2 cut(s) | 661, 938 |
| BstKTI | GATC | 3 cut(s) | 404, 532, 993 |
| BstMAI | GTCTC | 2 cut(s) | 19, 441 |
| BstMBI | GATC | 3 cut(s) | 401, 529, 990 |
| BstMCI | CGRYCG | 1 cut(s) | 9 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 665 |
| BstNI | CCWGG | 2 cut(s) | 227, 630 |
| BstNSI | RCATGY | 2 cut(s) | 331, 491 |
| BstSCI | CCNGG | 4 cut(s) | 225, 628, 866, 983 |
| BstSFI | CTRYAG | 1 cut(s) | 827 |
| BstUI | CGCG | 3 cut(s) | 497, 521, 936 |
| BstV1I | GCAGC | 7 cut(s) | 37, 291, 556, 648, 655, 707, 874 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 565 |
| Bsu15I | ATCGAT | 1 cut(s) | 978 |
| BsuRI | GGCC | 1 cut(s) | 258 |
| BsuTUI | ATCGAT | 1 cut(s) | 978 |
| BtgI | CCRYGG | 2 cut(s) | 318, 409 |
| BtrI | CACGTC | 1 cut(s) | 884 |
| BtsCI | GGATG | 3 cut(s) | 53, 376, 621 |
| BtsI | GCAGTG | 1 cut(s) | 951 |
| BtsIMutI | CAGTG | 2 cut(s) | 355, 951 |
| BveI | ACCTGC | 1 cut(s) | 105 |
| Cac8I | GCNNGC | 4 cut(s) | 102, 489, 666, 673 |
| CfoI | GCGC | 2 cut(s) | 661, 938 |
| Cfr13I | GGNCC | 2 cut(s) | 229, 523 |
| ClaI | ATCGAT | 1 cut(s) | 978 |
| CseI | GACGC | 3 cut(s) | 31, 503, 527 |
| CsiI | ACCWGGT | 1 cut(s) | 225 |
| Csp6I | GTAC | 3 cut(s) | 580, 947, 1035 |
| CviAII | CATG | 7 cut(s) | 221, 301, 319, 328, 488, 550, 957 |
| CviQI | GTAC | 3 cut(s) | 580, 947, 1035 |
| DdeI | CTNAG | 4 cut(s) | 59, 91, 544, 655 |
| DpnI | GATC | 3 cut(s) | 403, 531, 992 |
| DpnII | GATC | 3 cut(s) | 401, 529, 990 |
| DraI | TTTAAA | 1 cut(s) | 313 |
| Eam1104I | CTCTTC | 1 cut(s) | 777 |
| EarI | CTCTTC | 1 cut(s) | 777 |
| Eco130I | CCWWGG | 3 cut(s) | 283, 318, 897 |
| Eco47I | GGWCC | 2 cut(s) | 229, 523 |
| Eco47III | AGCGCT | 1 cut(s) | 660 |
| Eco57I | CTGAAG | 1 cut(s) | 165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 896 |
| EcoRI | GAATTC | 1 cut(s) | 791 |
| EcoRII | CCWGG | 2 cut(s) | 225, 628 |
| EcoT14I | CCWWGG | 3 cut(s) | 283, 318, 897 |
| EcoT22I | ATGCAT | 1 cut(s) | 249 |
| ErhI | CCWWGG | 3 cut(s) | 283, 318, 897 |
| Esp3I | CGTCTC | 1 cut(s) | 19 |
| FaeI | CATG | 7 cut(s) | 224, 304, 322, 331, 491, 553, 960 |
| FaqI | GGGAC | 1 cut(s) | 955 |
| FatI | CATG | 7 cut(s) | 220, 300, 318, 327, 487, 549, 956 |
| FauNDI | CATATG | 1 cut(s) | 46 |
| FblI | GTMKAC | 1 cut(s) | 9 |
| FokI | GGATG | 3 cut(s) | 40, 383, 608 |
| FspBI | CTAG | 3 cut(s) | 260, 863, 906 |
| GlaI | GCGC | 2 cut(s) | 660, 937 |
| GsuI | CTGGAG | 3 cut(s) | 331, 595, 783 |
| HaeII | RGCGCY | 1 cut(s) | 662 |
| HaeIII | GGCC | 1 cut(s) | 258 |
| HapII | CCGG | 5 cut(s) | 563, 587, 689, 868, 984 |
| HgaI | GACGC | 3 cut(s) | 31, 503, 527 |
| HhaI | GCGC | 2 cut(s) | 661, 938 |
| Hin1I | GRCGYC | 2 cut(s) | 12, 23 |
| Hin1II | CATG | 7 cut(s) | 224, 304, 322, 331, 491, 553, 960 |
| Hin6I | GCGC | 2 cut(s) | 659, 936 |
| HinP1I | GCGC | 2 cut(s) | 659, 936 |
| HincII | GTYRAC | 2 cut(s) | 10, 502 |
| HindII | GTYRAC | 2 cut(s) | 10, 502 |
| HinfI | GANTC | 4 cut(s) | 138, 364, 481, 608 |
| HpaII | CCGG | 5 cut(s) | 563, 587, 689, 868, 984 |
| HphI | GGTGA | 1 cut(s) | 800 |
| Hpy166II | GTNNAC | 8 cut(s) | 10, 111, 325, 502, 526, 949, 1016, 1035 |
| Hpy188I | TCNGA | 6 cut(s) | 94, 145, 161, 517, 745, 769 |
| Hpy188III | TCNNGA | 4 cut(s) | 368, 637, 779, 879 |
| Hpy8I | GTNNAC | 8 cut(s) | 10, 111, 325, 502, 526, 949, 1016, 1035 |
| Hpy99I | CGWCG | 3 cut(s) | 14, 25, 580 |
| HpyAV | CCTTC | 4 cut(s) | 282, 381, 593, 920 |
| HpyCH4IV | ACGT | 4 cut(s) | 12, 334, 575, 883 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 665 |
| HpyF3I | CTNAG | 4 cut(s) | 59, 91, 544, 655 |
| HpySE526I | ACGT | 4 cut(s) | 12, 334, 575, 883 |
| Hsp92I | GRCGYC | 2 cut(s) | 12, 23 |
| Hsp92II | CATG | 7 cut(s) | 224, 304, 322, 331, 491, 553, 960 |
| HspAI | GCGC | 2 cut(s) | 659, 936 |
| Kzo9I | GATC | 3 cut(s) | 401, 529, 990 |
| LmnI | GCTCC | 4 cut(s) | 283, 350, 595, 902 |
| Lsp1109I | GCAGC | 7 cut(s) | 37, 291, 556, 648, 655, 707, 874 |
| LweI | GCATC | 5 cut(s) | 62, 164, 630, 747, 970 |
| MabI | ACCWGGT | 1 cut(s) | 225 |
| MaeI | CTAG | 3 cut(s) | 260, 863, 906 |
| MaeII | ACGT | 4 cut(s) | 12, 334, 575, 883 |
| MalI | GATC | 3 cut(s) | 403, 531, 992 |
| MbiI | CCGCTC | 1 cut(s) | 278 |
| MboI | GATC | 3 cut(s) | 401, 529, 990 |
| MboII | GAAGA | 6 cut(s) | 158, 195, 289, 758, 794, 865 |
| MluCI | AATT | 5 cut(s) | 184, 265, 308, 375, 791 |
| MlyI | GAGTC | 3 cut(s) | 373, 475, 602 |
| MmeI | TCCRAC | 2 cut(s) | 256, 738 |
| Mph1103I | ATGCAT | 1 cut(s) | 249 |
| MseI | TTAA | 2 cut(s) | 312, 1002 |
| MspA1I | CMGCKG | 1 cut(s) | 668 |
| MspI | CCGG | 5 cut(s) | 563, 587, 689, 868, 984 |
| MspR9I | CCNGG | 4 cut(s) | 227, 630, 868, 985 |
| MvaI | CCWGG | 2 cut(s) | 227, 630 |
| MvnI | CGCG | 3 cut(s) | 497, 521, 936 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 665 |
| NciI | CCSGG | 2 cut(s) | 868, 985 |
| NcoI | CCATGG | 1 cut(s) | 318 |
| NdeI | CATATG | 1 cut(s) | 46 |
| NdeII | GATC | 3 cut(s) | 401, 529, 990 |
| NlaIII | CATG | 7 cut(s) | 224, 304, 322, 331, 491, 553, 960 |
| NlaIV | GGNNCC | 1 cut(s) | 591 |
| NsiI | ATGCAT | 1 cut(s) | 249 |
| NspI | RCATGY | 2 cut(s) | 331, 491 |
| PaeI | GCATGC | 1 cut(s) | 491 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 525 |
| PfeI | GAWTC | 1 cut(s) | 138 |
| PflFI | GACNNNGTC | 3 cut(s) | 227, 497, 521 |
| PleI | GAGTC | 3 cut(s) | 372, 475, 602 |
| PpsI | GAGTC | 3 cut(s) | 372, 475, 602 |
| Psp1406I | AACGTT | 1 cut(s) | 334 |
| Psp6I | CCWGG | 2 cut(s) | 225, 628 |
| PspGI | CCWGG | 2 cut(s) | 225, 628 |
| PspN4I | GGNNCC | 1 cut(s) | 591 |
| PspPI | GGNCC | 2 cut(s) | 229, 523 |
| PstI | CTGCAG | 1 cut(s) | 831 |
| PsyI | GACNNNGTC | 3 cut(s) | 227, 497, 521 |
| PvuII | CAGCTG | 1 cut(s) | 668 |
| RsaI | GTAC | 3 cut(s) | 581, 948, 1036 |
| RsaNI | GTAC | 3 cut(s) | 580, 947, 1035 |
| SalI | GTCGAC | 1 cut(s) | 8 |
| SaqAI | TTAA | 2 cut(s) | 312, 1002 |
| Sau3AI | GATC | 3 cut(s) | 401, 529, 990 |
| Sau96I | GGNCC | 2 cut(s) | 229, 523 |
| SchI | GAGTC | 3 cut(s) | 373, 475, 602 |
| ScrFI | CCNGG | 4 cut(s) | 227, 630, 868, 985 |
| SexAI | ACCWGGT | 1 cut(s) | 225 |
| SfaNI | GCATC | 5 cut(s) | 62, 164, 630, 747, 970 |
| SfcI | CTRYAG | 1 cut(s) | 827 |
| SinI | GGWCC | 2 cut(s) | 229, 523 |
| SphI | GCATGC | 1 cut(s) | 491 |
| Sse9I | AATT | 5 cut(s) | 184, 265, 308, 375, 791 |
| SsiI | CCGC | 9 cut(s) | 5, 26, 65, 74, 276, 485, 497, 521, 934 |
| SspMI | CTAG | 3 cut(s) | 260, 863, 906 |
| StyD4I | CCNGG | 4 cut(s) | 225, 628, 866, 983 |
| StyI | CCWWGG | 3 cut(s) | 283, 318, 897 |
| TaiI | ACGT | 4 cut(s) | 15, 337, 578, 886 |
| TaqI | TCGA | 4 cut(s) | 9, 131, 978, 993 |
| TasI | AATT | 5 cut(s) | 184, 265, 308, 375, 791 |
| TatI | WGTACW | 1 cut(s) | 1034 |
| TauI | GCSGC | 3 cut(s) | 28, 68, 278 |
| TfiI | GAWTC | 1 cut(s) | 138 |
| Tru1I | TTAA | 2 cut(s) | 312, 1002 |
| Tru9I | TTAA | 2 cut(s) | 312, 1002 |
| TscAI | CASTG | 2 cut(s) | 355, 958 |
| TseI | GCWGC | 7 cut(s) | 50, 304, 569, 661, 668, 695, 887 |
| TspDTI | ATGAA | 3 cut(s) | 289, 443, 710 |
| TspGWI | ACGGA | 2 cut(s) | 128, 426 |
| TspRI | CASTG | 2 cut(s) | 355, 958 |
| Tth111I | GACNNNGTC | 3 cut(s) | 227, 497, 521 |
| VpaK11BI | GGWCC | 2 cut(s) | 229, 523 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 896 |
| XapI | RAATTY | 2 cut(s) | 184, 791 |
| XceI | RCATGY | 2 cut(s) | 331, 491 |
| XmiI | GTMKAC | 1 cut(s) | 9 |
| XspI | CTAG | 3 cut(s) | 260, 863, 906 |
| ZraI | GACGTC | 1 cut(s) | 13 |
| Zsp2I | ATGCAT | 1 cut(s) | 249 |