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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 6 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 244, 413 |
| AciI | CCGC | 1 cut(s) | 116 |
| AcsI | RAATTY | 3 cut(s) | 139, 203, 398 |
| AcuI | CTGAAG | 2 cut(s) | 390, 542 |
| AcyI | GRCGYC | 1 cut(s) | 91 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 244, 413 |
| AflIII | ACRYGT | 1 cut(s) | 315 |
| AgsI | TTSAA | 2 cut(s) | 25, 145 |
| AhlI | ACTAGT | 1 cut(s) | 422 |
| AjnI | CCWGG | 1 cut(s) | 237 |
| AluBI | AGCT | 4 cut(s) | 30, 262, 548, 585 |
| AluI | AGCT | 4 cut(s) | 30, 262, 548, 585 |
| Alw21I | GWGCWC | 1 cut(s) | 32 |
| Alw26I | GTCTC | 2 cut(s) | 324, 499 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 244 |
| AoxI | GGCC | 1 cut(s) | 228 |
| ApoI | RAATTY | 3 cut(s) | 139, 203, 398 |
| AseI | ATTAAT | 1 cut(s) | 270 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 216 |
| AxyI | CCTNAGG | 2 cut(s) | 156, 174 |
| BanII | GRGCYC | 1 cut(s) | 32 |
| BbsI | GAAGAC | 1 cut(s) | 438 |
| Bbv12I | GWGCWC | 1 cut(s) | 32 |
| BccI | CCATC | 2 cut(s) | 356, 449 |
| BceAI | ACGGC | 2 cut(s) | 129, 215 |
| BciT130I | CCWGG | 1 cut(s) | 239 |
| BclI | TGATCA | 3 cut(s) | 7, 190, 327 |
| BcoDI | GTCTC | 2 cut(s) | 324, 499 |
| BcuI | ACTAGT | 1 cut(s) | 422 |
| BfaI | CTAG | 2 cut(s) | 423, 509 |
| BfuAI | ACCTGC | 1 cut(s) | 6 |
| BlpI | GCTNAGC | 1 cut(s) | 258 |
| Bme1390I | CCNGG | 1 cut(s) | 239 |
| BmrFI | CCNGG | 1 cut(s) | 239 |
| BpiI | GAAGAC | 1 cut(s) | 438 |
| BpmI | CTGGAG | 1 cut(s) | 390 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 258 |
| BpuEI | CTTGAG | 1 cut(s) | 36 |
| BsaHI | GRCGYC | 1 cut(s) | 91 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 244, 413 |
| Bse1I | ACTGG | 2 cut(s) | 249, 407 |
| Bse21I | CCTNAGG | 2 cut(s) | 156, 174 |
| BseBI | CCWGG | 1 cut(s) | 239 |
| BseGI | GGATG | 1 cut(s) | 348 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 244, 413 |
| BseMII | CTCAG | 5 cut(s) | 165, 170, 216, 249, 558 |
| BseNI | ACTGG | 2 cut(s) | 249, 407 |
| BseRI | GAGGAG | 2 cut(s) | 312, 515 |
| BshFI | GGCC | 1 cut(s) | 230 |
| BsiHKAI | GWGCWC | 1 cut(s) | 32 |
| BslFI | GGGAC | 2 cut(s) | 90, 503 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 244, 413 |
| BsmAI | GTCTC | 2 cut(s) | 324, 499 |
| BsmFI | GGGAC | 2 cut(s) | 90, 503 |
| BsnI | GGCC | 1 cut(s) | 230 |
| Bsp1286I | GDGCHC | 1 cut(s) | 32 |
| Bsp143I | GATC | 3 cut(s) | 7, 190, 327 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 258 |
| BspACI | CCGC | 1 cut(s) | 116 |
| BspANI | GGCC | 1 cut(s) | 230 |
| BspCNI | CTCAG | 5 cut(s) | 166, 169, 217, 250, 557 |
| BspMI | ACCTGC | 1 cut(s) | 6 |
| BspQI | GCTCTTC | 1 cut(s) | 20 |
| BsrI | ACTGG | 2 cut(s) | 249, 407 |
| BssMI | GATC | 3 cut(s) | 7, 190, 327 |
| BssNI | GRCGYC | 1 cut(s) | 91 |
| Bst2UI | CCWGG | 1 cut(s) | 239 |
| Bst4CI | ACNGT | 2 cut(s) | 442, 476 |
| Bst6I | CTCTTC | 2 cut(s) | 20, 492 |
| BstACI | GRCGYC | 1 cut(s) | 91 |
| BstC8I | GCNNGC | 2 cut(s) | 358, 583 |
| BstDEI | CTNAG | 5 cut(s) | 156, 174, 225, 258, 544 |
| BstF5I | GGATG | 1 cut(s) | 348 |
| BstKTI | GATC | 3 cut(s) | 10, 193, 330 |
| BstMAI | GTCTC | 2 cut(s) | 324, 499 |
| BstMBI | GATC | 3 cut(s) | 7, 190, 327 |
| BstNI | CCWGG | 1 cut(s) | 239 |
| BstNSI | RCATGY | 1 cut(s) | 319 |
| BstSCI | CCNGG | 1 cut(s) | 237 |
| BstV2I | GAAGAC | 1 cut(s) | 438 |
| Bsu36I | CCTNAGG | 2 cut(s) | 156, 174 |
| BsuRI | GGCC | 1 cut(s) | 230 |
| BtgZI | GCGATG | 1 cut(s) | 507 |
| BtsCI | GGATG | 1 cut(s) | 348 |
| BtsI | GCAGTG | 1 cut(s) | 381 |
| BtsIMutI | CAGTG | 3 cut(s) | 222, 381, 481 |
| BveI | ACCTGC | 1 cut(s) | 6 |
| Cac8I | GCNNGC | 2 cut(s) | 358, 583 |
| CaiI | CAGNNNCTG | 1 cut(s) | 244 |
| CseI | GACGC | 1 cut(s) | 80 |
| CviAII | CATG | 1 cut(s) | 316 |
| CviJI | RGCY | 7 cut(s) | 30, 230, 262, 295, 548, 562, 585 |
| CviKI_1 | RGCY | 7 cut(s) | 30, 230, 262, 295, 548, 562, 585 |
| DdeI | CTNAG | 5 cut(s) | 156, 174, 225, 258, 544 |
| DpnI | GATC | 3 cut(s) | 9, 192, 329 |
| DpnII | GATC | 3 cut(s) | 7, 190, 327 |
| Eam1104I | CTCTTC | 2 cut(s) | 20, 492 |
| EarI | CTCTTC | 2 cut(s) | 20, 492 |
| Ecl136II | GAGCTC | 1 cut(s) | 30 |
| Eco24I | GRGCYC | 1 cut(s) | 32 |
| Eco53kI | GAGCTC | 1 cut(s) | 30 |
| Eco57I | CTGAAG | 2 cut(s) | 390, 542 |
| Eco81I | CCTNAGG | 2 cut(s) | 156, 174 |
| EcoICRI | GAGCTC | 1 cut(s) | 30 |
| EcoRII | CCWGG | 1 cut(s) | 237 |
| EcoT38I | GRGCYC | 1 cut(s) | 32 |
| FaeI | CATG | 1 cut(s) | 319 |
| FaiI | YATR | 4 cut(s) | 60, 281, 317, 552 |
| FaqI | GGGAC | 2 cut(s) | 90, 503 |
| FatI | CATG | 1 cut(s) | 315 |
| FbaI | TGATCA | 3 cut(s) | 7, 190, 327 |
| FokI | GGATG | 1 cut(s) | 335 |
| FriOI | GRGCYC | 1 cut(s) | 32 |
| FspBI | CTAG | 2 cut(s) | 423, 509 |
| GsuI | CTGGAG | 1 cut(s) | 390 |
| HaeIII | GGCC | 1 cut(s) | 230 |
| HgaI | GACGC | 1 cut(s) | 80 |
| Hin1I | GRCGYC | 1 cut(s) | 91 |
| Hin1II | CATG | 1 cut(s) | 319 |
| HincII | GTYRAC | 4 cut(s) | 235, 286, 382, 472 |
| HindII | GTYRAC | 4 cut(s) | 235, 286, 382, 472 |
| HinfI | GANTC | 1 cut(s) | 542 |
| Hpy166II | GTNNAC | 7 cut(s) | 121, 235, 286, 345, 382, 445, 472 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 195, 327 |
| Hpy188III | TCNNGA | 3 cut(s) | 22, 53, 95 |
| Hpy8I | GTNNAC | 7 cut(s) | 121, 235, 286, 345, 382, 445, 472 |
| HpyAV | CCTTC | 1 cut(s) | 532 |
| HpyCH4III | ACNGT | 2 cut(s) | 442, 476 |
| HpyCH4V | TGCA | 4 cut(s) | 15, 163, 182, 356 |
| HpyF3I | CTNAG | 5 cut(s) | 156, 174, 225, 258, 544 |
| Hsp92I | GRCGYC | 1 cut(s) | 91 |
| Hsp92II | CATG | 1 cut(s) | 319 |
| Ksp22I | TGATCA | 3 cut(s) | 7, 190, 327 |
| Kzo9I | GATC | 3 cut(s) | 7, 190, 327 |
| LguI | GCTCTTC | 1 cut(s) | 20 |
| LmnI | GCTCC | 1 cut(s) | 409 |
| MaeI | CTAG | 2 cut(s) | 423, 509 |
| MalI | GATC | 3 cut(s) | 9, 192, 329 |
| MboI | GATC | 3 cut(s) | 7, 190, 327 |
| MboII | GAAGA | 4 cut(s) | 37, 224, 443, 509 |
| MhlI | GDGCHC | 1 cut(s) | 32 |
| MluCI | AATT | 3 cut(s) | 139, 203, 398 |
| MlyI | GAGTC | 1 cut(s) | 536 |
| MmeI | TCCRAC | 1 cut(s) | 394 |
| MnlI | CCTC | 6 cut(s) | 165, 169, 220, 333, 493, 567 |
| MroXI | GAANNNNTTC | 1 cut(s) | 216 |
| MseI | TTAA | 1 cut(s) | 270 |
| MspR9I | CCNGG | 1 cut(s) | 239 |
| MvaI | CCWGG | 1 cut(s) | 239 |
| NdeII | GATC | 3 cut(s) | 7, 190, 327 |
| NlaIII | CATG | 1 cut(s) | 319 |
| NspI | RCATGY | 1 cut(s) | 319 |
| PciI | ACATGT | 1 cut(s) | 315 |
| PciSI | GCTCTTC | 1 cut(s) | 20 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 88 |
| PdmI | GAANNNNTTC | 1 cut(s) | 216 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 244, 413 |
| PleI | GAGTC | 1 cut(s) | 536 |
| PpsI | GAGTC | 1 cut(s) | 536 |
| PscI | ACATGT | 1 cut(s) | 315 |
| PshBI | ATTAAT | 1 cut(s) | 270 |
| Psp124BI | GAGCTC | 1 cut(s) | 32 |
| Psp6I | CCWGG | 1 cut(s) | 237 |
| PspGI | CCWGG | 1 cut(s) | 237 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 201, 233 |
| PstNI | CAGNNNCTG | 1 cut(s) | 244 |
| SacI | GAGCTC | 1 cut(s) | 32 |
| SapI | GCTCTTC | 1 cut(s) | 20 |
| SaqAI | TTAA | 1 cut(s) | 270 |
| Sau3AI | GATC | 3 cut(s) | 7, 190, 327 |
| SchI | GAGTC | 1 cut(s) | 536 |
| ScrFI | CCNGG | 1 cut(s) | 239 |
| SduI | GDGCHC | 1 cut(s) | 32 |
| SetI | ASST | 9 cut(s) | 20, 32, 66, 129, 162, 180, 264, 550, 587 |
| SmlI | CTYRAG | 1 cut(s) | 51 |
| SmoI | CTYRAG | 1 cut(s) | 51 |
| SpeI | ACTAGT | 1 cut(s) | 422 |
| Sse9I | AATT | 3 cut(s) | 139, 203, 398 |
| SsiI | CCGC | 1 cut(s) | 116 |
| SspI | AATATT | 1 cut(s) | 568 |
| SspMI | CTAG | 2 cut(s) | 423, 509 |
| SstI | GAGCTC | 1 cut(s) | 32 |
| StyD4I | CCNGG | 1 cut(s) | 237 |
| TaaI | ACNGT | 2 cut(s) | 442, 476 |
| TasI | AATT | 3 cut(s) | 139, 203, 398 |
| Tru1I | TTAA | 1 cut(s) | 270 |
| Tru9I | TTAA | 1 cut(s) | 270 |
| TscAI | CASTG | 3 cut(s) | 229, 381, 481 |
| TspDTI | ATGAA | 5 cut(s) | 288, 411, 480, 498, 510 |
| TspRI | CASTG | 3 cut(s) | 229, 381, 481 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 244, 413 |
| VspI | ATTAAT | 1 cut(s) | 270 |
| XapI | RAATTY | 3 cut(s) | 139, 203, 398 |
| XceI | RCATGY | 1 cut(s) | 319 |
| XmnI | GAANNNNTTC | 1 cut(s) | 216 |
| XspI | CTAG | 2 cut(s) | 423, 509 |