Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 19 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 320 |
| AciI | CCGC | 1 cut(s) | 253 |
| AclWI | GGATC | 1 cut(s) | 180 |
| AcoI | YGGCCR | 1 cut(s) | 562 |
| AcsI | RAATTY | 1 cut(s) | 95 |
| AcuI | CTGAAG | 1 cut(s) | 663 |
| AcyI | GRCGYC | 1 cut(s) | 343 |
| AfaI | GTAC | 2 cut(s) | 294, 486 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 320 |
| AgsI | TTSAA | 4 cut(s) | 238, 536, 572, 656 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 645 |
| AjiI | CACGTC | 1 cut(s) | 338 |
| AluBI | AGCT | 3 cut(s) | 532, 629, 667 |
| AluI | AGCT | 3 cut(s) | 532, 629, 667 |
| Alw26I | GTCTC | 1 cut(s) | 579 |
| AlwI | GGATC | 1 cut(s) | 180 |
| Ama87I | CYCGRG | 1 cut(s) | 541 |
| AoxI | GGCC | 3 cut(s) | 184, 458, 562 |
| ApeKI | GCWGC | 1 cut(s) | 394 |
| ApoI | RAATTY | 1 cut(s) | 95 |
| AspA2I | CCTAGG | 1 cut(s) | 437 |
| AspS9I | GGNCC | 1 cut(s) | 458 |
| AsuHPI | GGTGA | 4 cut(s) | 24, 82, 338, 464 |
| AvaI | CYCGRG | 1 cut(s) | 541 |
| AvrII | CCTAGG | 1 cut(s) | 437 |
| BanI | GGYRCC | 1 cut(s) | 19 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 594, 626 |
| BbvI | GCAGC | 1 cut(s) | 406 |
| BccI | CCATC | 2 cut(s) | 443, 554 |
| BciVI | GTATCC | 1 cut(s) | 603 |
| BclI | TGATCA | 2 cut(s) | 246, 496 |
| BcoDI | GTCTC | 1 cut(s) | 579 |
| BfaI | CTAG | 5 cut(s) | 129, 195, 297, 438, 668 |
| BfmI | CTRYAG | 1 cut(s) | 702 |
| BfuI | GTATCC | 1 cut(s) | 603 |
| BisI | GCNGC | 2 cut(s) | 254, 395 |
| BlnI | CCTAGG | 1 cut(s) | 437 |
| BlsI | GCNGC | 2 cut(s) | 255, 396 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 645 |
| BmeT110I | CYCGRG | 1 cut(s) | 541 |
| BmgBI | CACGTC | 1 cut(s) | 338 |
| BmgT120I | GGNCC | 1 cut(s) | 458 |
| BmiI | GGNNCC | 1 cut(s) | 21 |
| BmrI | ACTGGG | 1 cut(s) | 140 |
| BmsI | GCATC | 1 cut(s) | 265 |
| BmuI | ACTGGG | 1 cut(s) | 140 |
| BpmI | CTGGAG | 1 cut(s) | 465 |
| Bpu10I | CCTNAGC | 3 cut(s) | 60, 390, 663 |
| BpuEI | CTTGAG | 1 cut(s) | 360 |
| BsaAI | YACGTR | 1 cut(s) | 488 |
| BsaBI | GATNNNNATC | 1 cut(s) | 245 |
| BsaHI | GRCGYC | 1 cut(s) | 343 |
| BsaI | GGTCTC | 1 cut(s) | 579 |
| BsaJI | CCNNGG | 3 cut(s) | 168, 437, 540 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 132, 162 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 320 |
| Bse1I | ACTGG | 4 cut(s) | 146, 325, 482, 511 |
| Bse8I | GATNNNNATC | 1 cut(s) | 245 |
| BseDI | CCNNGG | 3 cut(s) | 168, 437, 540 |
| BseJI | GATNNNNATC | 1 cut(s) | 245 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 320 |
| BseMII | CTCAG | 3 cut(s) | 74, 633, 710 |
| BseNI | ACTGG | 4 cut(s) | 146, 325, 482, 511 |
| BseXI | GCAGC | 1 cut(s) | 406 |
| BshFI | GGCC | 3 cut(s) | 186, 460, 564 |
| BshNI | GGYRCC | 1 cut(s) | 19 |
| BsiHKCI | CYCGRG | 1 cut(s) | 541 |
| BsiSI | CCGG | 1 cut(s) | 565 |
| BslFI | GGGAC | 3 cut(s) | 128, 371, 652 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 320 |
| BsmAI | GTCTC | 1 cut(s) | 579 |
| BsmFI | GGGAC | 3 cut(s) | 128, 371, 652 |
| BsmI | GAATGC | 1 cut(s) | 556 |
| BsnI | GGCC | 3 cut(s) | 186, 460, 564 |
| Bso31I | GGTCTC | 1 cut(s) | 579 |
| BsoBI | CYCGRG | 1 cut(s) | 541 |
| Bsp143I | GATC | 3 cut(s) | 172, 246, 496 |
| BspACI | CCGC | 1 cut(s) | 253 |
| BspANI | GGCC | 3 cut(s) | 186, 460, 564 |
| BspCNI | CTCAG | 3 cut(s) | 73, 632, 709 |
| BspLI | GGNNCC | 1 cut(s) | 21 |
| BspPI | GGATC | 1 cut(s) | 180 |
| BspT107I | GGYRCC | 1 cut(s) | 19 |
| BspTNI | GGTCTC | 1 cut(s) | 579 |
| BsrI | ACTGG | 4 cut(s) | 146, 325, 482, 511 |
| BssECI | CCNNGG | 3 cut(s) | 168, 437, 540 |
| BssMI | GATC | 3 cut(s) | 172, 246, 496 |
| BssNI | GRCGYC | 1 cut(s) | 343 |
| BssT1I | CCWWGG | 2 cut(s) | 168, 437 |
| Bst4CI | ACNGT | 2 cut(s) | 36, 292 |
| Bst6I | CTCTTC | 1 cut(s) | 356 |
| BstACI | GRCGYC | 1 cut(s) | 343 |
| BstBAI | YACGTR | 1 cut(s) | 488 |
| BstC8I | GCNNGC | 1 cut(s) | 627 |
| BstDEI | CTNAG | 5 cut(s) | 60, 390, 619, 663, 696 |
| BstKTI | GATC | 3 cut(s) | 175, 249, 499 |
| BstMAI | GTCTC | 1 cut(s) | 579 |
| BstMBI | GATC | 3 cut(s) | 172, 246, 496 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 183 |
| BstSFI | CTRYAG | 1 cut(s) | 702 |
| BstV1I | GCAGC | 1 cut(s) | 406 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 169 |
| BsuI | GTATCC | 1 cut(s) | 603 |
| BsuRI | GGCC | 3 cut(s) | 186, 460, 564 |
| BtrI | CACGTC | 1 cut(s) | 338 |
| BtsIMutI | CAGTG | 2 cut(s) | 153, 585 |
| Cac8I | GCNNGC | 1 cut(s) | 627 |
| Cfr13I | GGNCC | 1 cut(s) | 458 |
| CseI | GACGC | 1 cut(s) | 332 |
| Csp6I | GTAC | 2 cut(s) | 293, 485 |
| CviQI | GTAC | 2 cut(s) | 293, 485 |
| DdeI | CTNAG | 5 cut(s) | 60, 390, 619, 663, 696 |
| DpnI | GATC | 3 cut(s) | 174, 248, 498 |
| DpnII | GATC | 3 cut(s) | 172, 246, 496 |
| DriI | GACNNNNNGTC | 1 cut(s) | 645 |
| EaeI | YGGCCR | 1 cut(s) | 562 |
| Eam1104I | CTCTTC | 1 cut(s) | 356 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 645 |
| EarI | CTCTTC | 1 cut(s) | 356 |
| Eco130I | CCWWGG | 2 cut(s) | 168, 437 |
| Eco31I | GGTCTC | 1 cut(s) | 579 |
| Eco57I | CTGAAG | 1 cut(s) | 663 |
| Eco88I | CYCGRG | 1 cut(s) | 541 |
| EcoO109I | RGGNCCY | 1 cut(s) | 458 |
| EcoT14I | CCWWGG | 2 cut(s) | 168, 437 |
| ErhI | CCWWGG | 2 cut(s) | 168, 437 |
| FaiI | YATR | 8 cut(s) | 11, 120, 183, 222, 279, 384, 704, 714 |
| FaqI | GGGAC | 3 cut(s) | 128, 371, 652 |
| FbaI | TGATCA | 2 cut(s) | 246, 496 |
| Fnu4HI | GCNGC | 2 cut(s) | 254, 395 |
| Fsp4HI | GCNGC | 2 cut(s) | 254, 395 |
| FspBI | CTAG | 5 cut(s) | 129, 195, 297, 438, 668 |
| GluI | GCNGC | 2 cut(s) | 254, 395 |
| GsuI | CTGGAG | 1 cut(s) | 465 |
| HaeIII | GGCC | 3 cut(s) | 186, 460, 564 |
| HapII | CCGG | 1 cut(s) | 565 |
| HgaI | GACGC | 1 cut(s) | 332 |
| Hin1I | GRCGYC | 1 cut(s) | 343 |
| HincII | GTYRAC | 1 cut(s) | 214 |
| HindII | GTYRAC | 1 cut(s) | 214 |
| HinfI | GANTC | 5 cut(s) | 141, 371, 422, 568, 692 |
| HpaII | CCGG | 1 cut(s) | 565 |
| HphI | GGTGA | 4 cut(s) | 24, 82, 338, 464 |
| Hpy166II | GTNNAC | 2 cut(s) | 208, 214 |
| Hpy188I | TCNGA | 5 cut(s) | 45, 268, 501, 622, 675 |
| Hpy188III | TCNNGA | 1 cut(s) | 543 |
| Hpy8I | GTNNAC | 2 cut(s) | 208, 214 |
| Hpy99I | CGWCG | 1 cut(s) | 342 |
| HpyAV | CCTTC | 4 cut(s) | 449, 471, 596, 638 |
| HpyCH4III | ACNGT | 2 cut(s) | 36, 292 |
| HpyCH4IV | ACGT | 3 cut(s) | 210, 337, 487 |
| HpyCH4V | TGCA | 3 cut(s) | 273, 472, 554 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 183 |
| HpyF3I | CTNAG | 5 cut(s) | 60, 390, 619, 663, 696 |
| HpySE526I | ACGT | 3 cut(s) | 210, 337, 487 |
| Hsp92I | GRCGYC | 1 cut(s) | 343 |
| Ksp22I | TGATCA | 2 cut(s) | 246, 496 |
| Kzo9I | GATC | 3 cut(s) | 172, 246, 496 |
| LpnPI | CCDG | 6 cut(s) | 47, 159, 306, 492, 495, 578 |
| Lsp1109I | GCAGC | 1 cut(s) | 406 |
| LweI | GCATC | 1 cut(s) | 265 |
| MaeI | CTAG | 5 cut(s) | 129, 195, 297, 438, 668 |
| MaeII | ACGT | 3 cut(s) | 210, 337, 487 |
| MaeIII | GTNAC | 1 cut(s) | 344 |
| MalI | GATC | 3 cut(s) | 174, 248, 498 |
| MboI | GATC | 3 cut(s) | 172, 246, 496 |
| MboII | GAAGA | 3 cut(s) | 250, 373, 417 |
| MfeI | CAATTG | 1 cut(s) | 555 |
| MluCI | AATT | 5 cut(s) | 95, 135, 153, 411, 555 |
| MlyI | GAGTC | 1 cut(s) | 150 |
| MnlI | CCTC | 8 cut(s) | 6, 97, 227, 351, 357, 550, 628, 700 |
| MseI | TTAA | 1 cut(s) | 156 |
| MspI | CCGG | 1 cut(s) | 565 |
| MunI | CAATTG | 1 cut(s) | 555 |
| Mva1269I | GAATGC | 1 cut(s) | 556 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 183 |
| NdeII | GATC | 3 cut(s) | 172, 246, 496 |
| NlaIV | GGNNCC | 1 cut(s) | 21 |
| NmuCI | GTSAC | 1 cut(s) | 344 |
| PctI | GAATGC | 1 cut(s) | 556 |
| PfeI | GAWTC | 4 cut(s) | 371, 422, 568, 692 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 320 |
| PkrI | GCNGC | 2 cut(s) | 255, 396 |
| PleI | GAGTC | 1 cut(s) | 149 |
| PpsI | GAGTC | 1 cut(s) | 149 |
| Ppu21I | YACGTR | 1 cut(s) | 488 |
| PspN4I | GGNNCC | 1 cut(s) | 21 |
| PspPI | GGNCC | 1 cut(s) | 458 |
| RsaI | GTAC | 2 cut(s) | 294, 486 |
| RsaNI | GTAC | 2 cut(s) | 293, 485 |
| SaqAI | TTAA | 1 cut(s) | 156 |
| SatI | GCNGC | 2 cut(s) | 254, 395 |
| Sau3AI | GATC | 3 cut(s) | 172, 246, 496 |
| Sau96I | GGNCC | 1 cut(s) | 458 |
| SchI | GAGTC | 1 cut(s) | 150 |
| SfaNI | GCATC | 1 cut(s) | 265 |
| SfcI | CTRYAG | 1 cut(s) | 702 |
| SmlI | CTYRAG | 1 cut(s) | 375 |
| SmoI | CTYRAG | 1 cut(s) | 375 |
| Sse9I | AATT | 5 cut(s) | 95, 135, 153, 411, 555 |
| SsiI | CCGC | 1 cut(s) | 253 |
| SspMI | CTAG | 5 cut(s) | 129, 195, 297, 438, 668 |
| StyI | CCWWGG | 2 cut(s) | 168, 437 |
| TaaI | ACNGT | 2 cut(s) | 36, 292 |
| TaiI | ACGT | 3 cut(s) | 213, 340, 490 |
| TaqI | TCGA | 2 cut(s) | 109, 259 |
| TasI | AATT | 5 cut(s) | 95, 135, 153, 411, 555 |
| TauI | GCSGC | 1 cut(s) | 256 |
| TfiI | GAWTC | 4 cut(s) | 371, 422, 568, 692 |
| Tru1I | TTAA | 1 cut(s) | 156 |
| Tru9I | TTAA | 1 cut(s) | 156 |
| TscAI | CASTG | 2 cut(s) | 153, 585 |
| TseFI | GTSAC | 1 cut(s) | 344 |
| TseI | GCWGC | 1 cut(s) | 394 |
| Tsp45I | GTSAC | 1 cut(s) | 344 |
| TspDTI | ATGAA | 2 cut(s) | 453, 701 |
| TspGWI | ACGGA | 1 cut(s) | 622 |
| TspRI | CASTG | 2 cut(s) | 153, 585 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 320 |
| XapI | RAATTY | 1 cut(s) | 95 |
| XmaJI | CCTAGG | 1 cut(s) | 437 |
| XspI | CTAG | 5 cut(s) | 129, 195, 297, 438, 668 |