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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 166 |
| AccBSI | CCGCTC | 1 cut(s) | 87 |
| AccI | GTMKAC | 1 cut(s) | 346 |
| AccII | CGCG | 2 cut(s) | 6, 81 |
| AciI | CCGC | 7 cut(s) | 6, 81, 85, 146, 161, 266, 279 |
| AcoI | YGGCCR | 1 cut(s) | 82 |
| AcyI | GRCGYC | 1 cut(s) | 106 |
| AfaI | GTAC | 3 cut(s) | 139, 156, 240 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 240, 241 |
| AflIII | ACRYGT | 1 cut(s) | 436 |
| AgsI | TTSAA | 2 cut(s) | 126, 353 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 99 |
| AhlI | ACTAGT | 1 cut(s) | 65 |
| AluBI | AGCT | 4 cut(s) | 201, 299, 323, 402 |
| AluI | AGCT | 4 cut(s) | 201, 299, 323, 402 |
| Alw21I | GWGCWC | 1 cut(s) | 404 |
| Ama87I | CYCGRG | 1 cut(s) | 96 |
| AoxI | GGCC | 3 cut(s) | 82, 162, 243 |
| ApeKI | GCWGC | 5 cut(s) | 71, 182, 201, 204, 296 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 103, 135 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| AspS9I | GGNCC | 4 cut(s) | 11, 163, 243, 361 |
| AsuC2I | CCSGG | 1 cut(s) | 426 |
| AvaI | CYCGRG | 1 cut(s) | 96 |
| AvaII | GGWCC | 2 cut(s) | 11, 361 |
| BanII | GRGCYC | 1 cut(s) | 404 |
| Bbv12I | GWGCWC | 1 cut(s) | 404 |
| BbvI | GCAGC | 5 cut(s) | 58, 188, 194, 216, 308 |
| BccI | CCATC | 1 cut(s) | 26 |
| BclI | TGATCA | 2 cut(s) | 214, 271 |
| BcnI | CCSGG | 1 cut(s) | 426 |
| BcuI | ACTAGT | 1 cut(s) | 65 |
| BfaI | CTAG | 2 cut(s) | 66, 135 |
| BfmI | CTRYAG | 2 cut(s) | 180, 202 |
| BfuAI | ACCTGC | 1 cut(s) | 166 |
| BisI | GCNGC | 8 cut(s) | 72, 82, 85, 162, 183, 202, 205, 297 |
| BlpI | GCTNAGC | 1 cut(s) | 224 |
| BlsI | GCNGC | 8 cut(s) | 73, 83, 86, 163, 184, 203, 206, 298 |
| Bme1390I | CCNGG | 1 cut(s) | 426 |
| Bme18I | GGWCC | 2 cut(s) | 11, 361 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 99 |
| BmeT110I | CYCGRG | 1 cut(s) | 96 |
| BmgT120I | GGNCC | 4 cut(s) | 11, 163, 243, 361 |
| BmiI | GGNNCC | 2 cut(s) | 13, 363 |
| BmrFI | CCNGG | 1 cut(s) | 426 |
| BmrI | ACTGGG | 1 cut(s) | 230 |
| BmsI | GCATC | 1 cut(s) | 444 |
| BmuI | ACTGGG | 1 cut(s) | 230 |
| Bpu10I | CCTNAGC | 1 cut(s) | 336 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 224 |
| BpuEI | CTTGAG | 1 cut(s) | 246 |
| BpuMI | CCSGG | 1 cut(s) | 426 |
| BsaHI | GRCGYC | 1 cut(s) | 106 |
| BsaJI | CCNNGG | 1 cut(s) | 246 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 240, 241 |
| Bse1I | ACTGG | 1 cut(s) | 236 |
| BseDI | CCNNGG | 1 cut(s) | 246 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 240, 241 |
| BseMII | CTCAG | 1 cut(s) | 350 |
| BseNI | ACTGG | 1 cut(s) | 236 |
| BseRI | GAGGAG | 2 cut(s) | 394, 411 |
| BseX3I | CGGCCG | 1 cut(s) | 82 |
| BseXI | GCAGC | 5 cut(s) | 58, 188, 194, 216, 308 |
| BsgI | GTGCAG | 1 cut(s) | 335 |
| Bsh1236I | CGCG | 2 cut(s) | 6, 81 |
| Bsh1285I | CGRYCG | 1 cut(s) | 85 |
| BshFI | GGCC | 3 cut(s) | 84, 164, 245 |
| BsiEI | CGRYCG | 1 cut(s) | 85 |
| BsiHKAI | GWGCWC | 1 cut(s) | 404 |
| BsiHKCI | CYCGRG | 1 cut(s) | 96 |
| BsiSI | CCGG | 1 cut(s) | 425 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 240, 241 |
| BsnI | GGCC | 3 cut(s) | 84, 164, 245 |
| BsoBI | CYCGRG | 1 cut(s) | 96 |
| Bsp1286I | GDGCHC | 1 cut(s) | 404 |
| Bsp143I | GATC | 2 cut(s) | 214, 271 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 224 |
| BspACI | CCGC | 7 cut(s) | 6, 81, 85, 146, 161, 266, 279 |
| BspANI | GGCC | 3 cut(s) | 84, 164, 245 |
| BspCNI | CTCAG | 1 cut(s) | 349 |
| BspFNI | CGCG | 2 cut(s) | 6, 81 |
| BspLI | GGNNCC | 2 cut(s) | 13, 363 |
| BspMAI | CTGCAG | 2 cut(s) | 184, 206 |
| BspMI | ACCTGC | 1 cut(s) | 166 |
| BsrBI | CCGCTC | 1 cut(s) | 87 |
| BsrI | ACTGG | 1 cut(s) | 236 |
| BssECI | CCNNGG | 1 cut(s) | 246 |
| BssMI | GATC | 2 cut(s) | 214, 271 |
| BssNI | GRCGYC | 1 cut(s) | 106 |
| Bst4CI | ACNGT | 1 cut(s) | 119 |
| BstACI | GRCGYC | 1 cut(s) | 106 |
| BstC8I | GCNNGC | 1 cut(s) | 379 |
| BstDEI | CTNAG | 2 cut(s) | 224, 336 |
| BstDSI | CCRYGG | 1 cut(s) | 246 |
| BstFNI | CGCG | 2 cut(s) | 6, 81 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 2 cut(s) | 217, 274 |
| BstMBI | GATC | 2 cut(s) | 214, 271 |
| BstMCI | CGRYCG | 1 cut(s) | 85 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 68, 374 |
| BstNSI | RCATGY | 1 cut(s) | 440 |
| BstSCI | CCNGG | 1 cut(s) | 424 |
| BstSFI | CTRYAG | 2 cut(s) | 180, 202 |
| BstUI | CGCG | 2 cut(s) | 6, 81 |
| BstV1I | GCAGC | 5 cut(s) | 58, 188, 194, 216, 308 |
| BstZI | CGGCCG | 1 cut(s) | 82 |
| BsuRI | GGCC | 3 cut(s) | 84, 164, 245 |
| BtgI | CCRYGG | 1 cut(s) | 246 |
| BtsI | GCAGTG | 3 cut(s) | 318, 388, 408 |
| BtsIMutI | CAGTG | 3 cut(s) | 318, 388, 408 |
| BveI | ACCTGC | 1 cut(s) | 166 |
| Cac8I | GCNNGC | 1 cut(s) | 379 |
| CciNI | GCGGCCGC | 1 cut(s) | 82 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 4 cut(s) | 11, 163, 243, 361 |
| CseI | GACGC | 1 cut(s) | 95 |
| Csp6I | GTAC | 3 cut(s) | 138, 155, 239 |
| CviAII | CATG | 3 cut(s) | 287, 308, 437 |
| CviQI | GTAC | 3 cut(s) | 138, 155, 239 |
| DdeI | CTNAG | 2 cut(s) | 224, 336 |
| DpnI | GATC | 2 cut(s) | 216, 273 |
| DpnII | GATC | 2 cut(s) | 214, 271 |
| DriI | GACNNNNNGTC | 1 cut(s) | 99 |
| EaeI | YGGCCR | 1 cut(s) | 82 |
| EagI | CGGCCG | 1 cut(s) | 82 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 99 |
| Ecl136II | GAGCTC | 1 cut(s) | 402 |
| EclXI | CGGCCG | 1 cut(s) | 82 |
| Eco24I | GRGCYC | 1 cut(s) | 404 |
| Eco47I | GGWCC | 2 cut(s) | 11, 361 |
| Eco52I | CGGCCG | 1 cut(s) | 82 |
| Eco53kI | GAGCTC | 1 cut(s) | 402 |
| Eco88I | CYCGRG | 1 cut(s) | 96 |
| EcoICRI | GAGCTC | 1 cut(s) | 402 |
| EcoO109I | RGGNCCY | 1 cut(s) | 11 |
| EcoT38I | GRGCYC | 1 cut(s) | 404 |
| FaeI | CATG | 3 cut(s) | 290, 311, 440 |
| FaiI | YATR | 8 cut(s) | 192, 194, 230, 276, 288, 309, 438, 444 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 441, 473 |
| FatI | CATG | 3 cut(s) | 286, 307, 436 |
| FauI | CCCGC | 1 cut(s) | 153 |
| FauNDI | CATATG | 1 cut(s) | 192 |
| FbaI | TGATCA | 2 cut(s) | 214, 271 |
| FblI | GTMKAC | 1 cut(s) | 346 |
| Fnu4HI | GCNGC | 8 cut(s) | 72, 82, 85, 162, 183, 202, 205, 297 |
| FriOI | GRGCYC | 1 cut(s) | 404 |
| Fsp4HI | GCNGC | 8 cut(s) | 72, 82, 85, 162, 183, 202, 205, 297 |
| FspBI | CTAG | 2 cut(s) | 66, 135 |
| GlaI | GCGC | 1 cut(s) | 5 |
| GluI | GCNGC | 8 cut(s) | 72, 82, 85, 162, 183, 202, 205, 297 |
| HaeIII | GGCC | 3 cut(s) | 84, 164, 245 |
| HapII | CCGG | 1 cut(s) | 425 |
| HgaI | GACGC | 1 cut(s) | 95 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin1I | GRCGYC | 1 cut(s) | 106 |
| Hin1II | CATG | 3 cut(s) | 290, 311, 440 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HinfI | GANTC | 1 cut(s) | 94 |
| HpaII | CCGG | 1 cut(s) | 425 |
| Hpy166II | GTNNAC | 4 cut(s) | 140, 155, 347, 361 |
| Hpy188I | TCNGA | 1 cut(s) | 19 |
| Hpy188III | TCNNGA | 1 cut(s) | 338 |
| Hpy8I | GTNNAC | 4 cut(s) | 140, 155, 347, 361 |
| Hpy99I | CGWCG | 2 cut(s) | 111, 123 |
| HpyAV | CCTTC | 1 cut(s) | 124 |
| HpyCH4III | ACNGT | 1 cut(s) | 119 |
| HpyCH4V | TGCA | 6 cut(s) | 182, 204, 296, 311, 316, 381 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 68, 374 |
| HpyF3I | CTNAG | 2 cut(s) | 224, 336 |
| Hsp92I | GRCGYC | 1 cut(s) | 106 |
| Hsp92II | CATG | 3 cut(s) | 290, 311, 440 |
| HspAI | GCGC | 1 cut(s) | 4 |
| Ksp22I | TGATCA | 2 cut(s) | 214, 271 |
| Kzo9I | GATC | 2 cut(s) | 214, 271 |
| LmnI | GCTCC | 3 cut(s) | 92, 407, 424 |
| LpnPI | CCDG | 7 cut(s) | 171, 179, 249, 302, 323, 378, 438 |
| Lsp1109I | GCAGC | 5 cut(s) | 58, 188, 194, 216, 308 |
| LweI | GCATC | 1 cut(s) | 444 |
| MaeI | CTAG | 2 cut(s) | 66, 135 |
| MaeIII | GTNAC | 2 cut(s) | 394, 465 |
| MalI | GATC | 2 cut(s) | 216, 273 |
| MbiI | CCGCTC | 1 cut(s) | 87 |
| MboI | GATC | 2 cut(s) | 214, 271 |
| MhlI | GDGCHC | 1 cut(s) | 404 |
| MluCI | AATT | 1 cut(s) | 354 |
| MlyI | GAGTC | 1 cut(s) | 88 |
| MmeI | TCCRAC | 1 cut(s) | 370 |
| MnlI | CCTC | 5 cut(s) | 25, 92, 415, 432, 456 |
| MseI | TTAA | 1 cut(s) | 176 |
| MslI | CAYNNNNRTG | 1 cut(s) | 291 |
| MspI | CCGG | 1 cut(s) | 425 |
| MspR9I | CCNGG | 1 cut(s) | 426 |
| MvnI | CGCG | 2 cut(s) | 6, 81 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 68, 374 |
| NciI | CCSGG | 1 cut(s) | 426 |
| NdeI | CATATG | 1 cut(s) | 192 |
| NdeII | GATC | 2 cut(s) | 214, 271 |
| NlaIII | CATG | 3 cut(s) | 290, 311, 440 |
| NlaIV | GGNNCC | 2 cut(s) | 13, 363 |
| NmuCI | GTSAC | 2 cut(s) | 394, 465 |
| NotI | GCGGCCGC | 1 cut(s) | 82 |
| NspI | RCATGY | 1 cut(s) | 440 |
| PaeR7I | CTCGAG | 1 cut(s) | 96 |
| PciI | ACATGT | 1 cut(s) | 436 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 103, 115 |
| PkrI | GCNGC | 8 cut(s) | 73, 83, 86, 163, 184, 203, 206, 298 |
| PleI | GAGTC | 1 cut(s) | 88 |
| PpsI | GAGTC | 1 cut(s) | 88 |
| PpuMI | RGGWCCY | 1 cut(s) | 11 |
| PscI | ACATGT | 1 cut(s) | 436 |
| Psp124BI | GAGCTC | 1 cut(s) | 404 |
| Psp5II | RGGWCCY | 1 cut(s) | 11 |
| PspN4I | GGNNCC | 2 cut(s) | 13, 363 |
| PspPI | GGNCC | 4 cut(s) | 11, 163, 243, 361 |
| PspPPI | RGGWCCY | 1 cut(s) | 11 |
| PspXI | VCTCGAGB | 1 cut(s) | 96 |
| PstI | CTGCAG | 2 cut(s) | 184, 206 |
| RsaI | GTAC | 3 cut(s) | 139, 156, 240 |
| RsaNI | GTAC | 3 cut(s) | 138, 155, 239 |
| RseI | CAYNNNNRTG | 1 cut(s) | 291 |
| SacI | GAGCTC | 1 cut(s) | 404 |
| SaqAI | TTAA | 1 cut(s) | 176 |
| SatI | GCNGC | 8 cut(s) | 72, 82, 85, 162, 183, 202, 205, 297 |
| Sau3AI | GATC | 2 cut(s) | 214, 271 |
| Sau96I | GGNCC | 4 cut(s) | 11, 163, 243, 361 |
| SchI | GAGTC | 1 cut(s) | 88 |
| ScrFI | CCNGG | 1 cut(s) | 426 |
| SduI | GDGCHC | 1 cut(s) | 404 |
| SetI | ASST | 7 cut(s) | 103, 160, 203, 301, 325, 404, 467 |
| SfaNI | GCATC | 1 cut(s) | 444 |
| SfcI | CTRYAG | 2 cut(s) | 180, 202 |
| Sfr274I | CTCGAG | 1 cut(s) | 96 |
| SinI | GGWCC | 2 cut(s) | 11, 361 |
| SlaI | CTCGAG | 1 cut(s) | 96 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 291 |
| SmlI | CTYRAG | 2 cut(s) | 96, 261 |
| SmoI | CTYRAG | 2 cut(s) | 96, 261 |
| SpeI | ACTAGT | 1 cut(s) | 65 |
| Sse9I | AATT | 1 cut(s) | 354 |
| SsiI | CCGC | 7 cut(s) | 6, 81, 85, 146, 161, 266, 279 |
| SspMI | CTAG | 2 cut(s) | 66, 135 |
| SstI | GAGCTC | 1 cut(s) | 404 |
| StyD4I | CCNGG | 1 cut(s) | 424 |
| TaaI | ACNGT | 1 cut(s) | 119 |
| TaqI | TCGA | 2 cut(s) | 97, 109 |
| TasI | AATT | 1 cut(s) | 354 |
| TatI | WGTACW | 1 cut(s) | 137 |
| TauI | GCSGC | 3 cut(s) | 84, 87, 164 |
| Tru1I | TTAA | 1 cut(s) | 176 |
| Tru9I | TTAA | 1 cut(s) | 176 |
| TscAI | CASTG | 3 cut(s) | 318, 388, 415 |
| TseFI | GTSAC | 2 cut(s) | 394, 465 |
| TseI | GCWGC | 5 cut(s) | 71, 182, 201, 204, 296 |
| Tsp45I | GTSAC | 2 cut(s) | 394, 465 |
| TspDTI | ATGAA | 1 cut(s) | 431 |
| TspRI | CASTG | 3 cut(s) | 318, 388, 415 |
| VpaK11BI | GGWCC | 2 cut(s) | 11, 361 |
| XceI | RCATGY | 1 cut(s) | 440 |
| XhoI | CTCGAG | 1 cut(s) | 96 |
| XmiI | GTMKAC | 1 cut(s) | 346 |
| XspI | CTAG | 2 cut(s) | 66, 135 |