Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 819 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 286 |
| AccII | CGCG | 1 cut(s) | 806 |
| AciI | CCGC | 1 cut(s) | 364 |
| AclWI | GGATC | 2 cut(s) | 569, 830 |
| AcsI | RAATTY | 2 cut(s) | 162, 243 |
| AfaI | GTAC | 1 cut(s) | 142 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 286, 370, 523 |
| AflIII | ACRYGT | 2 cut(s) | 143, 589 |
| AgsI | TTSAA | 6 cut(s) | 103, 196, 383, 596, 641, 759 |
| AjnI | CCWGG | 2 cut(s) | 199, 258 |
| AleI | CACNNNNGTG | 1 cut(s) | 507 |
| AluBI | AGCT | 5 cut(s) | 35, 332, 613, 673, 813 |
| AluI | AGCT | 5 cut(s) | 35, 332, 613, 673, 813 |
| Alw26I | GTCTC | 1 cut(s) | 589 |
| AlwI | GGATC | 2 cut(s) | 569, 830 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 608 |
| AoxI | GGCC | 1 cut(s) | 746 |
| ApeKI | GCWGC | 1 cut(s) | 32 |
| ApoI | RAATTY | 2 cut(s) | 162, 243 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 10 |
| AspLEI | GCGC | 2 cut(s) | 806, 808 |
| AspS9I | GGNCC | 2 cut(s) | 746, 779 |
| AsuII | TTCGAA | 2 cut(s) | 177, 241 |
| AvaII | GGWCC | 1 cut(s) | 779 |
| BauI | CACGAG | 1 cut(s) | 507 |
| BbvCI | CCTCAGC | 1 cut(s) | 522 |
| BbvI | GCAGC | 1 cut(s) | 44 |
| BccI | CCATC | 1 cut(s) | 288 |
| BceAI | ACGGC | 1 cut(s) | 485 |
| BciT130I | CCWGG | 2 cut(s) | 201, 260 |
| BciVI | GTATCC | 1 cut(s) | 673 |
| BcoDI | GTCTC | 1 cut(s) | 589 |
| BfmI | CTRYAG | 2 cut(s) | 441, 826 |
| BfuAI | ACCTGC | 1 cut(s) | 819 |
| BfuI | GTATCC | 1 cut(s) | 673 |
| BglII | AGATCT | 1 cut(s) | 394 |
| BisI | GCNGC | 1 cut(s) | 33 |
| BlsI | GCNGC | 1 cut(s) | 34 |
| Bme1390I | CCNGG | 2 cut(s) | 201, 260 |
| Bme18I | GGWCC | 1 cut(s) | 779 |
| BmgT120I | GGNCC | 2 cut(s) | 746, 779 |
| BmiI | GGNNCC | 2 cut(s) | 44, 69 |
| BmrFI | CCNGG | 2 cut(s) | 201, 260 |
| BmsI | GCATC | 5 cut(s) | 32, 280, 375, 715, 737 |
| BpmI | CTGGAG | 1 cut(s) | 606 |
| Bpu10I | CCTNAGC | 1 cut(s) | 522 |
| Bpu14I | TTCGAA | 2 cut(s) | 177, 241 |
| BpuEI | CTTGAG | 1 cut(s) | 213 |
| BsaJI | CCNNGG | 4 cut(s) | 56, 298, 467, 666 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 124, 154 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 286, 370, 523 |
| Bse3DI | GCAATG | 1 cut(s) | 314 |
| BseBI | CCWGG | 2 cut(s) | 201, 260 |
| BseDI | CCNNGG | 4 cut(s) | 56, 298, 467, 666 |
| BseGI | GGATG | 1 cut(s) | 271 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 286, 370, 523 |
| BseMI | GCAATG | 1 cut(s) | 314 |
| BseMII | CTCAG | 2 cut(s) | 513, 600 |
| BsePI | GCGCGC | 1 cut(s) | 804 |
| BseXI | GCAGC | 1 cut(s) | 44 |
| Bsh1236I | CGCG | 1 cut(s) | 806 |
| BshFI | GGCC | 1 cut(s) | 748 |
| BslFI | GGGAC | 1 cut(s) | 322 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 286, 370, 523 |
| BsmAI | GTCTC | 1 cut(s) | 589 |
| BsmFI | GGGAC | 1 cut(s) | 322 |
| BsnI | GGCC | 1 cut(s) | 748 |
| Bsp119I | TTCGAA | 2 cut(s) | 177, 241 |
| Bsp143I | GATC | 3 cut(s) | 394, 561, 822 |
| Bsp19I | CCATGG | 2 cut(s) | 56, 298 |
| BspACI | CCGC | 1 cut(s) | 364 |
| BspANI | GGCC | 1 cut(s) | 748 |
| BspCNI | CTCAG | 2 cut(s) | 514, 601 |
| BspFNI | CGCG | 1 cut(s) | 806 |
| BspLI | GGNNCC | 2 cut(s) | 44, 69 |
| BspMAI | CTGCAG | 1 cut(s) | 830 |
| BspMI | ACCTGC | 1 cut(s) | 819 |
| BspPI | GGATC | 2 cut(s) | 569, 830 |
| BspT104I | TTCGAA | 2 cut(s) | 177, 241 |
| BsrDI | GCAATG | 1 cut(s) | 314 |
| BssECI | CCNNGG | 4 cut(s) | 56, 298, 467, 666 |
| BssHII | GCGCGC | 1 cut(s) | 804 |
| BssMI | GATC | 3 cut(s) | 394, 561, 822 |
| BssSI | CACGAG | 1 cut(s) | 507 |
| BssT1I | CCWWGG | 2 cut(s) | 56, 298 |
| Bst2BI | CACGAG | 1 cut(s) | 507 |
| Bst2UI | CCWGG | 2 cut(s) | 201, 260 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 29 |
| BstBI | TTCGAA | 2 cut(s) | 177, 241 |
| BstC8I | GCNNGC | 4 cut(s) | 364, 615, 714, 806 |
| BstDEI | CTNAG | 3 cut(s) | 386, 522, 609 |
| BstDSI | CCRYGG | 4 cut(s) | 56, 298, 467, 666 |
| BstF5I | GGATG | 1 cut(s) | 271 |
| BstFNI | CGCG | 1 cut(s) | 806 |
| BstHHI | GCGC | 2 cut(s) | 806, 808 |
| BstKTI | GATC | 3 cut(s) | 397, 564, 825 |
| BstMAI | GTCTC | 1 cut(s) | 589 |
| BstMBI | GATC | 3 cut(s) | 394, 561, 822 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 29, 810 |
| BstNI | CCWGG | 2 cut(s) | 201, 260 |
| BstNSI | RCATGY | 2 cut(s) | 147, 593 |
| BstSCI | CCNGG | 2 cut(s) | 199, 258 |
| BstSFI | CTRYAG | 2 cut(s) | 441, 826 |
| BstUI | CGCG | 1 cut(s) | 806 |
| BstV1I | GCAGC | 1 cut(s) | 44 |
| BstX2I | RGATCY | 2 cut(s) | 394, 822 |
| BstYI | RGATCY | 2 cut(s) | 394, 822 |
| BsuI | GTATCC | 1 cut(s) | 673 |
| BsuRI | GGCC | 1 cut(s) | 748 |
| BtgI | CCRYGG | 4 cut(s) | 56, 298, 467, 666 |
| BtsCI | GGATG | 1 cut(s) | 271 |
| BtsIMutI | CAGTG | 1 cut(s) | 699 |
| BveI | ACCTGC | 1 cut(s) | 819 |
| Cac8I | GCNNGC | 4 cut(s) | 364, 615, 714, 806 |
| CaiI | CAGNNNCTG | 1 cut(s) | 608 |
| CfoI | GCGC | 2 cut(s) | 806, 808 |
| Cfr13I | GGNCC | 2 cut(s) | 746, 779 |
| CseI | GACGC | 1 cut(s) | 332 |
| CsiI | ACCWGGT | 1 cut(s) | 199 |
| Csp6I | GTAC | 1 cut(s) | 141 |
| CviAII | CATG | 8 cut(s) | 57, 118, 144, 286, 299, 431, 518, 590 |
| CviQI | GTAC | 1 cut(s) | 141 |
| DdeI | CTNAG | 3 cut(s) | 386, 522, 609 |
| DpnI | GATC | 3 cut(s) | 396, 563, 824 |
| DpnII | GATC | 3 cut(s) | 394, 561, 822 |
| Eco130I | CCWWGG | 2 cut(s) | 56, 298 |
| Eco47I | GGWCC | 1 cut(s) | 779 |
| EcoRII | CCWGG | 2 cut(s) | 199, 258 |
| EcoT14I | CCWWGG | 2 cut(s) | 56, 298 |
| EcoT22I | ATGCAT | 1 cut(s) | 730 |
| ErhI | CCWWGG | 2 cut(s) | 56, 298 |
| FaeI | CATG | 8 cut(s) | 60, 121, 147, 289, 302, 434, 521, 593 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 12, 44 |
| FaqI | GGGAC | 1 cut(s) | 322 |
| FatI | CATG | 8 cut(s) | 56, 117, 143, 285, 298, 430, 517, 589 |
| FauI | CCCGC | 1 cut(s) | 371 |
| FauNDI | CATATG | 1 cut(s) | 793 |
| Fnu4HI | GCNGC | 1 cut(s) | 33 |
| FokI | GGATG | 1 cut(s) | 258 |
| Fsp4HI | GCNGC | 1 cut(s) | 33 |
| GlaI | GCGC | 2 cut(s) | 805, 807 |
| GluI | GCNGC | 1 cut(s) | 33 |
| GsuI | CTGGAG | 1 cut(s) | 606 |
| HaeIII | GGCC | 1 cut(s) | 748 |
| HgaI | GACGC | 1 cut(s) | 332 |
| HhaI | GCGC | 2 cut(s) | 806, 808 |
| Hin1II | CATG | 8 cut(s) | 60, 121, 147, 289, 302, 434, 521, 593 |
| Hin6I | GCGC | 2 cut(s) | 804, 806 |
| HinP1I | GCGC | 2 cut(s) | 804, 806 |
| HinfI | GANTC | 4 cut(s) | 11, 238, 490, 755 |
| Hpy166II | GTNNAC | 2 cut(s) | 634, 788 |
| Hpy188I | TCNGA | 1 cut(s) | 18 |
| Hpy188III | TCNNGA | 6 cut(s) | 249, 392, 479, 509, 551, 623 |
| Hpy8I | GTNNAC | 2 cut(s) | 634, 788 |
| HpyAV | CCTTC | 1 cut(s) | 221 |
| HpyCH4V | TGCA | 3 cut(s) | 32, 728, 828 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 29, 810 |
| HpyF3I | CTNAG | 3 cut(s) | 386, 522, 609 |
| Hsp92II | CATG | 8 cut(s) | 60, 121, 147, 289, 302, 434, 521, 593 |
| HspAI | GCGC | 2 cut(s) | 804, 806 |
| Kzo9I | GATC | 3 cut(s) | 394, 561, 822 |
| LmnI | GCTCC | 2 cut(s) | 670, 818 |
| Lsp1109I | GCAGC | 1 cut(s) | 44 |
| LweI | GCATC | 5 cut(s) | 32, 280, 375, 715, 737 |
| MabI | ACCWGGT | 1 cut(s) | 199 |
| MaeIII | GTNAC | 2 cut(s) | 500, 763 |
| MalI | GATC | 3 cut(s) | 396, 563, 824 |
| MboI | GATC | 3 cut(s) | 394, 561, 822 |
| MboII | GAAGA | 3 cut(s) | 122, 135, 653 |
| MflI | RGATCY | 2 cut(s) | 394, 822 |
| MluCI | AATT | 3 cut(s) | 162, 243, 347 |
| MnlI | CCTC | 6 cut(s) | 225, 285, 491, 517, 527, 652 |
| Mph1103I | ATGCAT | 1 cut(s) | 730 |
| MroXI | GAANNNNTTC | 1 cut(s) | 10 |
| MseI | TTAA | 2 cut(s) | 254, 678 |
| MslI | CAYNNNNRTG | 1 cut(s) | 507 |
| MspR9I | CCNGG | 2 cut(s) | 201, 260 |
| MvaI | CCWGG | 2 cut(s) | 201, 260 |
| MvnI | CGCG | 1 cut(s) | 806 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 29, 810 |
| NcoI | CCATGG | 2 cut(s) | 56, 298 |
| NdeI | CATATG | 1 cut(s) | 793 |
| NdeII | GATC | 3 cut(s) | 394, 561, 822 |
| NlaIII | CATG | 8 cut(s) | 60, 121, 147, 289, 302, 434, 521, 593 |
| NlaIV | GGNNCC | 2 cut(s) | 44, 69 |
| NmuCI | GTSAC | 2 cut(s) | 500, 763 |
| NsiI | ATGCAT | 1 cut(s) | 730 |
| NspI | RCATGY | 2 cut(s) | 147, 593 |
| NspV | TTCGAA | 2 cut(s) | 177, 241 |
| OliI | CACNNNNGTG | 1 cut(s) | 507 |
| PauI | GCGCGC | 1 cut(s) | 804 |
| PciI | ACATGT | 2 cut(s) | 143, 589 |
| PdmI | GAANNNNTTC | 1 cut(s) | 10 |
| PfeI | GAWTC | 4 cut(s) | 11, 238, 490, 755 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 286 |
| PkrI | GCNGC | 1 cut(s) | 34 |
| PscI | ACATGT | 2 cut(s) | 143, 589 |
| Psp6I | CCWGG | 2 cut(s) | 199, 258 |
| PspGI | CCWGG | 2 cut(s) | 199, 258 |
| PspN4I | GGNNCC | 2 cut(s) | 44, 69 |
| PspPI | GGNCC | 2 cut(s) | 746, 779 |
| PstI | CTGCAG | 1 cut(s) | 830 |
| PstNI | CAGNNNCTG | 1 cut(s) | 608 |
| PsuI | RGATCY | 2 cut(s) | 394, 822 |
| PteI | GCGCGC | 1 cut(s) | 804 |
| RsaI | GTAC | 1 cut(s) | 142 |
| RsaNI | GTAC | 1 cut(s) | 141 |
| RseI | CAYNNNNRTG | 1 cut(s) | 507 |
| SaqAI | TTAA | 2 cut(s) | 254, 678 |
| SatI | GCNGC | 1 cut(s) | 33 |
| Sau3AI | GATC | 3 cut(s) | 394, 561, 822 |
| Sau96I | GGNCC | 2 cut(s) | 746, 779 |
| ScrFI | CCNGG | 2 cut(s) | 201, 260 |
| SexAI | ACCWGGT | 1 cut(s) | 199 |
| SfaNI | GCATC | 5 cut(s) | 32, 280, 375, 715, 737 |
| SfcI | CTRYAG | 2 cut(s) | 441, 826 |
| SfuI | TTCGAA | 2 cut(s) | 177, 241 |
| SinI | GGWCC | 1 cut(s) | 779 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 507 |
| SmlI | CTYRAG | 1 cut(s) | 228 |
| SmoI | CTYRAG | 1 cut(s) | 228 |
| Sse9I | AATT | 3 cut(s) | 162, 243, 347 |
| SsiI | CCGC | 1 cut(s) | 364 |
| SspI | AATATT | 1 cut(s) | 580 |
| StyD4I | CCNGG | 2 cut(s) | 199, 258 |
| StyI | CCWWGG | 2 cut(s) | 56, 298 |
| TaqI | TCGA | 2 cut(s) | 177, 241 |
| TasI | AATT | 3 cut(s) | 162, 243, 347 |
| TfiI | GAWTC | 4 cut(s) | 11, 238, 490, 755 |
| Tru1I | TTAA | 2 cut(s) | 254, 678 |
| Tru9I | TTAA | 2 cut(s) | 254, 678 |
| TscAI | CASTG | 1 cut(s) | 706 |
| TseFI | GTSAC | 2 cut(s) | 500, 763 |
| TseI | GCWGC | 1 cut(s) | 32 |
| Tsp45I | GTSAC | 2 cut(s) | 500, 763 |
| TspDTI | ATGAA | 2 cut(s) | 86, 536 |
| TspGWI | ACGGA | 1 cut(s) | 683 |
| TspRI | CASTG | 1 cut(s) | 706 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 286 |
| VpaK11BI | GGWCC | 1 cut(s) | 779 |
| XapI | RAATTY | 2 cut(s) | 162, 243 |
| XceI | RCATGY | 2 cut(s) | 147, 593 |
| XmnI | GAANNNNTTC | 1 cut(s) | 10 |
| Zsp2I | ATGCAT | 1 cut(s) | 730 |