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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 229 |
| AccB1I | GGYRCC | 1 cut(s) | 229 |
| AciI | CCGC | 1 cut(s) | 151 |
| AcsI | RAATTY | 3 cut(s) | 238, 264, 311 |
| AfaI | GTAC | 2 cut(s) | 231, 401 |
| AflIII | ACRYGT | 1 cut(s) | 292 |
| AgsI | TTSAA | 1 cut(s) | 243 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 230, 262 |
| AluBI | AGCT | 1 cut(s) | 362 |
| AluI | AGCT | 1 cut(s) | 362 |
| AoxI | GGCC | 1 cut(s) | 152 |
| ApeKI | GCWGC | 1 cut(s) | 359 |
| ApoI | RAATTY | 3 cut(s) | 238, 264, 311 |
| Asp718I | GGTACC | 1 cut(s) | 229 |
| AsuC2I | CCSGG | 1 cut(s) | 377 |
| BanI | GGYRCC | 1 cut(s) | 229 |
| BanII | GRGCYC | 1 cut(s) | 202 |
| BbvI | GCAGC | 1 cut(s) | 371 |
| BcnI | CCSGG | 1 cut(s) | 377 |
| BfaI | CTAG | 3 cut(s) | 87, 126, 183 |
| BisI | GCNGC | 2 cut(s) | 152, 360 |
| BlsI | GCNGC | 2 cut(s) | 153, 361 |
| Bme1390I | CCNGG | 1 cut(s) | 377 |
| BmiI | GGNNCC | 2 cut(s) | 78, 231 |
| BmrFI | CCNGG | 1 cut(s) | 377 |
| BmsI | GCATC | 3 cut(s) | 34, 55, 76 |
| BpuMI | CCSGG | 1 cut(s) | 377 |
| Bse118I | RCCGGY | 1 cut(s) | 79 |
| BseGI | GGATG | 4 cut(s) | 25, 46, 67, 226 |
| BseMII | CTCAG | 3 cut(s) | 215, 225, 350 |
| BseRI | GAGGAG | 1 cut(s) | 247 |
| BseXI | GCAGC | 1 cut(s) | 371 |
| BshFI | GGCC | 1 cut(s) | 154 |
| BshNI | GGYRCC | 1 cut(s) | 229 |
| BsiSI | CCGG | 3 cut(s) | 80, 376, 391 |
| BsnI | GGCC | 1 cut(s) | 154 |
| Bsp1286I | GDGCHC | 1 cut(s) | 202 |
| BspACI | CCGC | 1 cut(s) | 151 |
| BspANI | GGCC | 1 cut(s) | 154 |
| BspCNI | CTCAG | 3 cut(s) | 214, 226, 349 |
| BspLI | GGNNCC | 2 cut(s) | 78, 231 |
| BspT107I | GGYRCC | 1 cut(s) | 229 |
| BsrFI | RCCGGY | 1 cut(s) | 79 |
| BssAI | RCCGGY | 1 cut(s) | 79 |
| Bst4CI | ACNGT | 1 cut(s) | 322 |
| Bst6I | CTCTTC | 1 cut(s) | 159 |
| BstDEI | CTNAG | 3 cut(s) | 201, 234, 336 |
| BstEII | GGTNACC | 1 cut(s) | 250 |
| BstF5I | GGATG | 4 cut(s) | 25, 46, 67, 226 |
| BstPI | GGTNACC | 1 cut(s) | 250 |
| BstSCI | CCNGG | 1 cut(s) | 375 |
| BstV1I | GCAGC | 1 cut(s) | 371 |
| BsuRI | GGCC | 1 cut(s) | 154 |
| BtsCI | GGATG | 4 cut(s) | 25, 46, 67, 226 |
| BtsIMutI | CAGTG | 1 cut(s) | 126 |
| Cfr10I | RCCGGY | 1 cut(s) | 79 |
| Csp6I | GTAC | 2 cut(s) | 230, 400 |
| CviJI | RGCY | 7 cut(s) | 79, 125, 154, 172, 200, 362, 394 |
| CviKI_1 | RGCY | 7 cut(s) | 79, 125, 154, 172, 200, 362, 394 |
| CviQI | GTAC | 2 cut(s) | 230, 400 |
| DdeI | CTNAG | 3 cut(s) | 201, 234, 336 |
| Eam1104I | CTCTTC | 1 cut(s) | 159 |
| EarI | CTCTTC | 1 cut(s) | 159 |
| Eco24I | GRGCYC | 1 cut(s) | 202 |
| Eco32I | GATATC | 1 cut(s) | 117 |
| Eco91I | GGTNACC | 1 cut(s) | 250 |
| EcoO65I | GGTNACC | 1 cut(s) | 250 |
| EcoRI | GAATTC | 1 cut(s) | 238 |
| EcoRV | GATATC | 1 cut(s) | 117 |
| EcoT38I | GRGCYC | 1 cut(s) | 202 |
| FaiI | YATR | 3 cut(s) | 131, 279, 350 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 156, 188 |
| Fnu4HI | GCNGC | 2 cut(s) | 152, 360 |
| FokI | GGATG | 4 cut(s) | 12, 33, 54, 233 |
| FriOI | GRGCYC | 1 cut(s) | 202 |
| Fsp4HI | GCNGC | 2 cut(s) | 152, 360 |
| FspBI | CTAG | 3 cut(s) | 87, 126, 183 |
| GluI | GCNGC | 2 cut(s) | 152, 360 |
| HaeIII | GGCC | 1 cut(s) | 154 |
| HapII | CCGG | 3 cut(s) | 80, 376, 391 |
| HinfI | GANTC | 2 cut(s) | 4, 179 |
| HpaII | CCGG | 3 cut(s) | 80, 376, 391 |
| Hpy188I | TCNGA | 2 cut(s) | 204, 339 |
| HpyAV | CCTTC | 2 cut(s) | 45, 139 |
| HpyCH4III | ACNGT | 1 cut(s) | 322 |
| HpyCH4IV | ACGT | 1 cut(s) | 292 |
| HpyF3I | CTNAG | 3 cut(s) | 201, 234, 336 |
| HpySE526I | ACGT | 1 cut(s) | 292 |
| KpnI | GGTACC | 1 cut(s) | 233 |
| LmnI | GCTCC | 2 cut(s) | 37, 76 |
| LpnPI | CCDG | 6 cut(s) | 42, 63, 84, 93, 246, 389 |
| Lsp1109I | GCAGC | 1 cut(s) | 371 |
| LweI | GCATC | 3 cut(s) | 34, 55, 76 |
| MaeI | CTAG | 3 cut(s) | 87, 126, 183 |
| MaeII | ACGT | 1 cut(s) | 292 |
| MaeIII | GTNAC | 2 cut(s) | 82, 250 |
| MboII | GAAGA | 6 cut(s) | 176, 200, 293, 314, 317, 320 |
| MhlI | GDGCHC | 1 cut(s) | 202 |
| MluCI | AATT | 5 cut(s) | 106, 138, 238, 264, 311 |
| MnlI | CCTC | 8 cut(s) | 160, 211, 265, 268, 292, 333, 373, 398 |
| MseI | TTAA | 2 cut(s) | 137, 210 |
| MslI | CAYNNNNRTG | 1 cut(s) | 276 |
| MspI | CCGG | 3 cut(s) | 80, 376, 391 |
| MspR9I | CCNGG | 1 cut(s) | 377 |
| NciI | CCSGG | 1 cut(s) | 377 |
| NlaIV | GGNNCC | 2 cut(s) | 78, 231 |
| NmuCI | GTSAC | 1 cut(s) | 82 |
| PfeI | GAWTC | 2 cut(s) | 4, 179 |
| PkrI | GCNGC | 2 cut(s) | 153, 361 |
| PspEI | GGTNACC | 1 cut(s) | 250 |
| PspN4I | GGNNCC | 2 cut(s) | 78, 231 |
| RsaI | GTAC | 2 cut(s) | 231, 401 |
| RsaNI | GTAC | 2 cut(s) | 230, 400 |
| RseI | CAYNNNNRTG | 1 cut(s) | 276 |
| SaqAI | TTAA | 2 cut(s) | 137, 210 |
| SatI | GCNGC | 2 cut(s) | 152, 360 |
| ScrFI | CCNGG | 1 cut(s) | 377 |
| SduI | GDGCHC | 1 cut(s) | 202 |
| SetI | ASST | 6 cut(s) | 150, 235, 257, 295, 364, 390 |
| SfaNI | GCATC | 3 cut(s) | 34, 55, 76 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 276 |
| Sse9I | AATT | 5 cut(s) | 106, 138, 238, 264, 311 |
| SsiI | CCGC | 1 cut(s) | 151 |
| SspMI | CTAG | 3 cut(s) | 87, 126, 183 |
| StyD4I | CCNGG | 1 cut(s) | 375 |
| TaaI | ACNGT | 1 cut(s) | 322 |
| TaiI | ACGT | 1 cut(s) | 295 |
| TasI | AATT | 5 cut(s) | 106, 138, 238, 264, 311 |
| TauI | GCSGC | 1 cut(s) | 154 |
| TfiI | GAWTC | 2 cut(s) | 4, 179 |
| Tru1I | TTAA | 2 cut(s) | 137, 210 |
| Tru9I | TTAA | 2 cut(s) | 137, 210 |
| TscAI | CASTG | 1 cut(s) | 126 |
| TseFI | GTSAC | 1 cut(s) | 82 |
| TseI | GCWGC | 1 cut(s) | 359 |
| Tsp45I | GTSAC | 1 cut(s) | 82 |
| TspDTI | ATGAA | 3 cut(s) | 17, 237, 294 |
| TspRI | CASTG | 1 cut(s) | 126 |
| XapI | RAATTY | 3 cut(s) | 238, 264, 311 |
| XspI | CTAG | 3 cut(s) | 87, 126, 183 |